GCF_900637985.1 - 3 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_LR134479.1 (Rothia aeria)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	lassopeptide,lanthipeptide-class-ii	246,015	287,919
Region 2	terpene-precursor	867,370	888,503
Region 3	NRPS	1,575,724	1,619,980	High	enterobactin	NRPS:Type I

NZ_LR134479.1 - Region 1 - lanthipeptide-class-ii,lassopeptide

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Location: 246,015 - 287,919 nt. (total: 41,905 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

Lasso peptides

TFBS Finder

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase reductase

EL250_RS01215: alkyl hydroperoxide reductase/ Thiol specific

smcogs: SMCOG1196: alkyl hydroperoxide reductase/ Thiol specific

EL250_RS01295: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

Transferases: acetyltransferase

EL250_RS01220: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Hydrolases: hydrolase

EL250_RS01250: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Lyases: cyclase

EL250_RS01325: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

EL250_RS01335: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Ligases: ligase

EL250_RS01240: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001555.4	0.32	ribosomal	colicin V	Escherichia coli chi7122
BGC0002698.2	0.31	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000517.4	0.30	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0000575.5	0.30	ribosomal	lariatin	Rhodococcus jostii
BGC0000504.5	0.29	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0001889.2	0.29	ribosomal	fusilassin	Thermobifida fusca YX
BGC0001210.5	0.29	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000589.5	0.29	ribosomal	microcin M	Escherichia coli Nissle 1917
BGC0000588.5	0.29	ribosomal	microcin L	Escherichia coli
BGC0000500.5	0.28	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001176.4	0.61	ribosomal	streptomonomicin	Streptomonospora alba
BGC0001229.4	0.59	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1
BGC0002110.3	0.56	ribosomal	birimositide	Streptomyces rimosus subsp. rimosus WC-3908
BGC0002698.2	0.54	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0002150.2	0.54	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.54	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0001555.4	0.52	ribosomal	colicin V	Escherichia coli chi7122
BGC0001634.3	0.51	ribosomal	keywimysin	Streptomyces sp. NRRL F-5702
BGC0000575.5	0.51	ribosomal	lariatin	Rhodococcus jostii
BGC0001549.3	0.51	ribosomal	citrulassin B	Streptomyces aurantiacus JA 4570

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for EL250_RS01325

EL250_RS01320

Leader:

MAKDTMFMEELTDQELEQVNGG

Core:

CYSSCGYYGGSYGGSYGGSYGGSSSSYYGGSYSGGGYYYAAVGYGYSC

Core with dehydrations:

CysYDhaDhaCysGYYGGDhaYGGDhaYGGDhaYGGDhaDhaDhaDhaYYGGDhaYDhaGGGYYYAAVGYGYDhaCys

antiSMASH-DB 4.0 matches for EL250_RS01320

100.0%

NZ_LR134479.1and 1 other:

NZ_LR134479.1 Lanthipeptides: EL250_RS01320_lanthipeptide

Lanthipeptides: EL250_RS01320_lanthipeptide

CYSSCGYYGGSYGGSYGGSYGGSSSSYYGGSYSGGGYYYAAVGYGYSC

97.9%

NZ_CP079819.1and 1 other:

NZ_CP079819.1 Lanthipeptides: LPB401_RS00090_lanthipeptide

Lanthipeptides: LPB401_RS00090_lanthipeptide

CYSSCGYYGGSYGGSYGGSYGGSSSSYYGGSYSGGGYYYAAVGYGYSC

CYSSCGYYGGSYGGSYGGSY GSSSSYYGGSYSGGGYYYAAVGYGYSC

CYSSCGYYGGSYGGSYGGSYCGSSSSYYGGSYSGGGYYYAAVGYGYSC

97.9%

NZ_KI518028.1and 1 other:

NZ_KI518028.1 Lanthipeptides: HMPREF0742_RS01810_lanthipeptide

Lanthipeptides: HMPREF0742_RS01810_lanthipeptide

CYSSCGYYGGSYGGSYGGSYGGSSSSYYGGSYSGGGYYYAAVGYGYSC

CYSSCGYYGGSYGGSYGGSYGGS+SSYYGGSYSGGGYYYAAVGYGYSC

CYSSCGYYGGSYGGSYGGSYGGSNSSYYGGSYSGGGYYYAAVGYGYSC

MIBiG 4.0 matches for EL250_RS01320

8.3%

BGC0000581.6 (AAD28494.1):

Lasso peptide: microcin J25

GSSSSYYG

G+ S+YG

GTPISFYG

EL250_RS01330

Leader:

MPKDITLLEEISDQELGQVNGG

Core:

GSHGGGYYSSVGGGSYGGGYCGGGCGGYGYGFGFWGGSFS

Core with dehydrations:

GDhaHGGGYYDhaDhaVGGGDhaYGGGYCysGGGCysGGYGYGFGFWGGDhaFDha

antiSMASH-DB 4.0 matches for EL250_RS01330

100.0%

NZ_CP068102.1and 7 others:

NZ_CP068102.1 Lanthipeptides: I6I94_RS04840_lanthipeptideNZ_CP079819.1 Lanthipeptides: LPB401_RS00100_lanthipeptideNZ_CP079819.1 Lanthipeptides: LPB401_RS00100_lanthipeptideNZ_KI518028.1 Lanthipeptides: HMPREF0742_RS01820_lanthipeptideNZ_KI518028.1 Lanthipeptides: HMPREF0742_RS01820_lanthipeptideNZ_LR134479.1 Lanthipeptides: EL250_RS01330_lanthipeptideNZ_LR134479.1 Lanthipeptides: EL250_RS01330_lanthipeptide

Lanthipeptides: I6I94_RS04840_lanthipeptide

GSHGGGYYSSVGGGSYGGGYCGGGCGGYGYGFGFWGGSFS

18.2%

NC_014643.1and 16 others:

NC_014643.1 Lanthipeptides: HMPREF0733_RS05060_lanthipeptideNZ_CP054018.1 Lanthipeptides: FOC60_RS00340_lanthipeptideNZ_CP079201.1 Lanthipeptides: LPB409_RS01335_lanthipeptideNZ_JVLW01000024.1 Lanthipeptides: ADD47_RS04725_lanthipeptideNZ_JVXR01000038.1 Lanthipeptides: ADK06_RS05015_lanthipeptideNZ_KV793075.1 Lanthipeptides: HMPREF3164_RS06945_lanthipeptideNZ_KV795763.1 Lanthipeptides: HMPREF2554_RS09025_lanthipeptideNZ_KV795763.1 Lanthipeptides: HMPREF2554_RS09025_lanthipeptideNZ_KV797630.1 Lanthipeptides: HMPREF2609_RS06005_lanthipeptideNZ_KV797887.1 Lanthipeptides: HMPREF2625_RS00515_lanthipeptideNZ_KV802313.1 Lanthipeptides: HMPREF2756_RS06530_lanthipeptideNZ_KV804818.1 Lanthipeptides: HMPREF2804_RS09475_lanthipeptideNZ_KV804818.1 Lanthipeptides: HMPREF2804_RS09475_lanthipeptideNZ_KV815277.1 Lanthipeptides: HMPREF2958_RS01940_lanthipeptideNZ_KV817389.1 Lanthipeptides: HMPREF2981_RS04625_lanthipeptideNZ_KV820076.1 Lanthipeptides: HMPREF3016_RS03055_lanthipeptide

Lanthipeptides: HMPREF0733_RS05060_lanthipeptide

YGFGFWGGS

+GFGFWGGS

FGFGFWGGS

MIBiG 4.0 matches for EL250_RS01330

15.0%

BGC0002322.3 (CEJ66604.1):

Lasso peptide: citrocin

YCGGGCGGYG

+ GGG G YG

FIGGGVGRYG

allorf_0261182_0261298

Leader:

MNEIQLIGA

Core:

PQPPPTRGTRGALRRAPAQECIKIRYVRG

Core with dehydrations:

PQPPPDhbRGDhbRGALRRAPAQECysIKIRYVRG

antiSMASH-DB 4.0 matches for allorf_0261182_0261298

100.0%

NZ_LR134479.1:

Lanthipeptides: allorf_0261182_0261298_lanthipeptide

PQPPPTRGTRGALRRAPAQECIKIRYVRG

96.6%

NZ_KI518028.1:

Lanthipeptides: allorf_287397_287513_lanthipeptide

PQPPPTRGTRGALRRAPAQECIKIRYVRG

PQPPPTRGTRGALRRAPAQ+CIKIRYVRG

PQPPPTRGTRGALRRAPAQKCIKIRYVRG

93.1%

NZ_CP068102.1:

Lanthipeptides: allorf_1185663_1185779_lanthipeptide

PQPPPTRGTRGALRRAPAQECIKIRYVRG

PQPPPTRGTRGALRRAPA++CIKIRYVRG

PQPPPTRGTRGALRRAPARKCIKIRYVRG

with 4 more sequence matches

27.0%

NZ_CP013219.1:

Lanthipeptides: allorf_0179059_0179217_lanthipeptide

PQPPPTRGTRGALRRAPAQ

P PP G RGA RR P +

PVPPADGGPRGAGRRVPCR

24.1%

NZ_CP026498.1:

Lanthipeptides: allorf_00224338_00224481_lanthipeptide

PTRGTRGALRRAPAQ

P G+RG +RR P +

PRNGSRGGVRRRPGE

22.2%

NZ_FNPT01000012.1:

Lanthipeptides: allorf_041529_041675_lanthipeptide

PQPPPTRGTRGALRRAP

P P R R ALR P

PHPAFARADRAALRTGP

15.2%

NZ_LR881952.1and 4 others:

NZ_LR881952.1 Lanthipeptides: allorf_5341425_5341625_lanthipeptideNZ_LR881952.1 Lanthipeptides: allorf_5341425_5341625_lanthipeptideNZ_LR881952.1 Lanthipeptides: allorf_5341425_5341625_lanthipeptideNZ_LZQS01000002.1 Lanthipeptides: allorf_595585_595785_lanthipeptide

Lanthipeptides: allorf_5341425_5341625_lanthipeptide

APAQECIKIRYVRG

APA E +++RY G

APAWETLRLRYGAG

allorf_0265595_0265780

Leader:

MTKYCMSHAKYYGA

Core:

ESQKLMFVIYCSPPSARGYTQNARIMSPGVKLWLAGRQPSPVAGCPR

Core with dehydrations:

EDhaQKLMFVIYCysDhaPPDhaARGYDhbQNARIMDhaPGVKLWLAGRQPDhaPVAGCysPR

antiSMASH-DB 4.0 matches for allorf_0265595_0265780

100.0%

NZ_CP079819.1and 5 others:

NZ_CP079819.1 Lanthipeptides: allorf_0008942_0009127_lanthipeptideNZ_KI518028.1 Lanthipeptides: allorf_291815_292000_lanthipeptideNZ_KI518028.1 Lanthipeptides: allorf_291815_292000_lanthipeptideNZ_LR134479.1 Lanthipeptides: allorf_0265595_0265780_lanthipeptideNZ_LR134479.1 Lanthipeptides: allorf_0265595_0265780_lanthipeptide

Lanthipeptides: allorf_0008942_0009127_lanthipeptide

ESQKLMFVIYCSPPSARGYTQNARIMSPGVKLWLAGRQPSPVAGCPR

34.0%

NZ_CP068102.1and 1 other:

NZ_CP068102.1 Lanthipeptides: allorf_1181240_1181359_lanthipeptide

Lanthipeptides: allorf_1181240_1181359_lanthipeptide

ESQKLMFVIYCSPPSARGYT

ESQKLMFVIYCS P RG T

ESQKLMFVIYCS-PHLRGDT

14.9%

NZ_CP054018.1:

Lanthipeptides: allorf_0093622_0093795_lanthipeptide

SQKLMFVIYCSPPSARGYTQNAR

+ L+F + C S RG+ R

NNALIFFVCCRSSSIRGHVSRIR

allorf_0265627_0265695

Leader:

MYPLADGGEQYM

Core:

TNINFCDSAP

Core with dehydrations:

DhbNINFCysDDhaAP

antiSMASH-DB 4.0 matches for allorf_0265627_0265695

100.0%

NZ_CP079819.1and 5 others:

NZ_CP079819.1 Lanthipeptides: allorf_0008974_0009042_lanthipeptideNZ_KI518028.1 Lanthipeptides: allorf_291847_291915_lanthipeptideNZ_KI518028.1 Lanthipeptides: allorf_291847_291915_lanthipeptideNZ_LR134479.1 Lanthipeptides: allorf_0265627_0265695_lanthipeptideNZ_LR134479.1 Lanthipeptides: allorf_0265627_0265695_lanthipeptide

Lanthipeptides: allorf_0008974_0009042_lanthipeptide

TNINFCDSAP

allorf_0265696_0265836

Leader:

MCRWRIRAVLAS

Core:

CSLKPDHHLGHPATGEGCRPASQSFTPGLIILAF

Core with dehydrations:

CysDhaLKPDHHLGHPADhbGEGCysRPADhaQDhaFDhbPGLIILAF

antiSMASH-DB 4.0 matches for allorf_0265696_0265836

100.0%

NZ_CP068102.1and 7 others:

NZ_CP068102.1 Lanthipeptides: allorf_1181119_1181259_lanthipeptideNZ_CP079819.1 Lanthipeptides: allorf_0009043_0009246_lanthipeptideNZ_CP079819.1 Lanthipeptides: allorf_0009043_0009246_lanthipeptideNZ_KI518028.1 Lanthipeptides: allorf_291916_292056_lanthipeptideNZ_KI518028.1 Lanthipeptides: allorf_291916_292056_lanthipeptideNZ_LR134479.1 Lanthipeptides: allorf_0265696_0265836_lanthipeptideNZ_LR134479.1 Lanthipeptides: allorf_0265696_0265836_lanthipeptide

Lanthipeptides: allorf_1181119_1181259_lanthipeptide

CSLKPDHHLGHPATGEGCRPASQSFTPGLIILAF

23.5%

NZ_CP069338.1and 1 other:

NZ_CP069338.1 Lanthipeptides: allorf_09109175_09109414_lanthipeptide

Lanthipeptides: allorf_09109175_09109414_lanthipeptide

HLGHPATGEGCRPASQSFT

G P EG P +SFT

EFGAPPLKEGNHPCLESFT

23.5%

NZ_SNZH01000011.1:

Lanthipeptides: allorf_218796_219029_lanthipeptide

TGEGCRPASQSFT

TG GCR A S T

TGSGCRKAGPSHT

with 1 more sequence matches

14.6%

NZ_LIPB01000002.1:

Lanthipeptides: allorf_1135461_1135619_lanthipeptide

HPATGEGCR

HP G GCR

HPWGGSGCR

MIBiG 4.0 matches for allorf_0265696_0265836

11.8%

BGC0000580.3 (EGD17355.1):

Lasso peptide: xanthomonin II or xanthomonin I

GHPATGE

G P GE

GGPLAGE

8.8%

BGC0000566.5 (SPy_0738):

LAP: streptolysin S

FTPG

+TPG

YTPG

allorf_0265791_0265895

Leader:

MFLHPARRLLIGG

Core:

GLLGVPSCAGGAFAPYLLRVP

Core with dehydrations:

GLLGVPDhaCysAGGAFAPYLLRVP

antiSMASH-DB 4.0 matches for allorf_0265791_0265895

100.0%

NZ_CP068102.1and 5 others:

NZ_CP068102.1 Lanthipeptides: allorf_1181060_1181164_lanthipeptideNZ_CP079819.1 Lanthipeptides: allorf_0009138_0009242_lanthipeptideNZ_CP079819.1 Lanthipeptides: allorf_0009138_0009242_lanthipeptideNZ_LR134479.1 Lanthipeptides: allorf_0265791_0265895_lanthipeptideNZ_LR134479.1 Lanthipeptides: allorf_0265791_0265895_lanthipeptide

Lanthipeptides: allorf_1181060_1181164_lanthipeptide

GLLGVPSCAGGAFAPYLLRVP

26.9%

NC_014483.2and 23 others:

NC_014622.2 Lanthipeptides: PPSC2_RS36205_lanthipeptideNC_017542.1 Lanthipeptides: PPM_RS07465_lanthipeptideNZ_CP006872.1 Lanthipeptides: PPSQR21_RS07740_lanthipeptideNZ_CP023711.1 Lanthipeptides: CPY53_RS22235_lanthipeptideNZ_CP025957.1 Lanthipeptides: C1A50_RS07530_lanthipeptideNZ_CP029370.1 Lanthipeptides: DI243_RS07495_lanthipeptideNZ_CP040829.1 Lanthipeptides: FGY93_RS00500_lanthipeptideNZ_CP049598.1 Lanthipeptides: G7L40_RS00115_lanthipeptideNZ_CP061172.1 Lanthipeptides: IAQ67_RS07480_lanthipeptideNZ_CP073682.1 Lanthipeptides: KAI36_RS07745_lanthipeptideNZ_CP084033.1 Lanthipeptides: LAZ93_RS07550_lanthipeptideNZ_CP085682.1 Lanthipeptides: LJR53_RS07370_lanthipeptideNZ_CP086373.1 Lanthipeptides: LMH85_RS07780_lanthipeptideNZ_CP092779.1 Lanthipeptides: MJ749_RS07440_lanthipeptideNZ_CP097766.1 Lanthipeptides: MF620_RS07485_lanthipeptideNZ_CP102865.1 Lanthipeptides: NWS42_RS21195_lanthipeptideNZ_CP109848.1 Lanthipeptides: OF859_RS22650_lanthipeptideNZ_CP113973.1 Lanthipeptides: OYT09_RS07785_lanthipeptideNZ_JAAMNR010000001.1 Lanthipeptides: G9G54_RS03500_lanthipeptideNZ_JWJJ01000002.1 Lanthipeptides: U707_RS10390_lanthipeptideNZ_PNXQ01000013.1 Lanthipeptides: C1I60_RS14430_lanthipeptideNZ_POVS01000009.1 Lanthipeptides: C1T21_RS10630_lanthipeptideNZ_POVT01000009.1 Lanthipeptides: C1T20_RS05900_lanthipeptide

Lanthipeptides: PPE_RS07020_lanthipeptide

GLLGVPSCAGGAF

GL+ PSCA G

GLICTPSCATGTL

23.1%

NC_023037.2and 6 others:

NZ_CP017967.2 Lanthipeptides: PPYC1_RS07755_lanthipeptideNZ_CP017968.3 Lanthipeptides: PPYC2_RS08330_lanthipeptideNZ_CP073683.1 Lanthipeptides: KAI37_RS07495_lanthipeptideNZ_FOYG01000001.1 Lanthipeptides: BM358_RS03525_lanthipeptideNZ_JAKVDC010000001.1 Lanthipeptides: MKN04_RS02755_lanthipeptideNZ_PTJM01000020.1 Lanthipeptides: C5G87_RS02825_lanthipeptide

Lanthipeptides: X809_RS07820_lanthipeptide

GLLGVPSCAGGAF

G++ PSCA G

GIICTPSCATGTL

with 2 more sequence matches

15.7%

NZ_WWJS01000019.1:

Lanthipeptides: GT018_RS20925_lanthipeptide

GLLGVPSCAGGAFAPY

G VP +GG APY

GCQSVPDQSGGVVAPY

14.3%

NC_017765.1and 1 other:

NZ_KQ948355.1 Lanthipeptides: AQJ11_RS50945_lanthipeptide

Lanthipeptides: SHJG_RS52475_lanthipeptide

VPSCAGGAFAPY

VP+CAGG P+

VPACAGGIARPF

MIBiG 4.0 matches for allorf_0265791_0265895

19.0%

BGC0000503.5 (cinA):

Lanthipeptide: cinnamycin

SCAGGAFA

SC+ G F

SCSFGPFT

allorf_0269996_0270211

Leader:

MEIMMRTYCILPRWFDTVEGLARVAISLLQGRELIVGVLLRMQRMDSIESFRKLILGS

Core:

YCVIGTVLHSHIS

Core with dehydrations:

YCysVIGDhbVLHDhaHIDha

antiSMASH-DB 4.0 matches for allorf_0269996_0270211

100.0%

NZ_CP068102.1and 7 others:

NZ_CP068102.1 Lanthipeptides: allorf_1176779_1176994_lanthipeptideNZ_CP079819.1 Lanthipeptides: allorf_0013342_0013557_lanthipeptideNZ_CP079819.1 Lanthipeptides: allorf_0013342_0013557_lanthipeptideNZ_KI518028.1 Lanthipeptides: allorf_295232_295447_lanthipeptideNZ_KI518028.1 Lanthipeptides: allorf_295232_295447_lanthipeptideNZ_LR134479.1 Lanthipeptides: allorf_0269996_0270211_lanthipeptideNZ_LR134479.1 Lanthipeptides: allorf_0269996_0270211_lanthipeptide

Lanthipeptides: allorf_1176779_1176994_lanthipeptide

YCVIGTVLHSHIS

24.0%

NZ_JACHNX010000043.1:

Lanthipeptides: allorf_0139_0258_lanthipeptide

CVIGTVLHSH

CVIG HS

CVIGEADHSE

10.3%

NZ_CP045142.1:

Lanthipeptides: GBQ70_RS07075_lanthipeptide

YCVIGTVLHSHIS

YC T LH H S

YCDTPTRLHRHES

allorf_0270096_0270281

Leader:

MLCALIALYYG

Core:

HHITKWCDIKWKLRICESVKLFLSHNRSPISAFGSSQCYPFFASSIEHPQ

Core with dehydrations:

HHIDhbKWCysDIKWKLRICysEDhaVKLFLDhaHNRDhaPIDhaAFGDhaDhaQCysYPFFADhaDhaIEHPQ

antiSMASH-DB 4.0 matches for allorf_0270096_0270281

100.0%

NZ_KI518028.1and 3 others:

NZ_KI518028.1 Lanthipeptides: allorf_295332_295517_lanthipeptideNZ_LR134479.1 Lanthipeptides: allorf_0270096_0270281_lanthipeptideNZ_LR134479.1 Lanthipeptides: allorf_0270096_0270281_lanthipeptide

Lanthipeptides: allorf_295332_295517_lanthipeptide

HHITKWCDIKWKLRICESVKLFLSHNRSPISAFGSSQCYPFFASSIEHPQ

98.0%

NZ_CP068102.1and 1 other:

NZ_CP068102.1 Lanthipeptides: allorf_1176709_1176894_lanthipeptide

Lanthipeptides: allorf_1176709_1176894_lanthipeptide

HHITKWCDIKWKLRICESVKLFLSHNRSPISAFGSSQCYPFFASSIEHPQ

HHITKWCDIKWKLRICESVKLFLSHNRSPISAFGSS+CYPFFASSIEHPQ

HHITKWCDIKWKLRICESVKLFLSHNRSPISAFGSSRCYPFFASSIEHPQ

12.0%

NZ_JAMDLZ010000027.1:

Lanthipeptides: M5W82_RS15515_lanthipeptide

KWCDIKWKLRIC

KWC + W+ IC

KWCTLTWECSIC

with 3 more sequence matches

12.0%

NZ_LT745762.1and 1 other:

NZ_LT745762.1 Lanthipeptides: B2C77_RS00795_lanthipeptide

Lanthipeptides: B2C77_RS00795_lanthipeptide

KWCDIKWKLRICES

KWC + W+ IC +

KWCTVTWECSICPT

10.0%

NZ_BQME02000001.1and 1 other:

NZ_BQME02000001.1 Lanthipeptides: MFF31_RS04105_lanthipeptide

Lanthipeptides: MFF31_RS04105_lanthipeptide

KWCDIKWKLRICESVKLF

K+C + W+ +C ++ F

KYCTLTWECSVCPTLTCF

10.0%

NZ_CP013979.1:

Lanthipeptides: ATC03_RS01475_lanthipeptide

NRSPISAFGSSQCYPFFAS

+P+ FG C P+++

GTNPLGTFGCCWCVPWWSG

allorf_0270410_0270481

Leader:

MVENRDLASS

Core:

SPKHSTIKNCSRK

Core with dehydrations:

DhaPKHDhaDhbIKNCysDhaRK

antiSMASH-DB 4.0 matches for allorf_0270410_0270481

100.0%

NZ_LR134479.1and 1 other:

NZ_LR134479.1 Lanthipeptides: allorf_0270410_0270481_lanthipeptide

Lanthipeptides: allorf_0270410_0270481_lanthipeptide

SPKHSTIKNCSRK

92.3%

NZ_CP079819.1and 3 others:

NZ_CP079819.1 Lanthipeptides: allorf_0013756_0013827_lanthipeptideNZ_KI518028.1 Lanthipeptides: allorf_295646_295717_lanthipeptideNZ_KI518028.1 Lanthipeptides: allorf_295646_295717_lanthipeptide

Lanthipeptides: allorf_0013756_0013827_lanthipeptide

SPKHSTIKNCSRK

SPKHS IKNCSRK

SPKHSAIKNCSRK

84.6%

NZ_CP068102.1and 1 other:

NZ_CP068102.1 Lanthipeptides: allorf_1176509_1176580_lanthipeptide

Lanthipeptides: allorf_1176509_1176580_lanthipeptide

SPKHSTIKNCSRK

SPK+S IKNCSRK

SPKYSAIKNCSRK

Lanthipeptide(s) for EL250_RS01335

EL250_RS01320

Leader:

MAKDTMFMEELTDQELEQVNGG

Core:

CYSSCGYYGGSYGGSYGGSYGGSSSSYYGGSYSGGGYYYAAVGYGYSC

Core with dehydrations:

CysYDhaDhaCysGYYGGDhaYGGDhaYGGDhaYGGDhaDhaDhaDhaYYGGDhaYDhaGGGYYYAAVGYGYDhaCys

antiSMASH-DB 4.0 matches for EL250_RS01320

100.0%

NZ_LR134479.1and 1 other:

NZ_LR134479.1 Lanthipeptides: EL250_RS01320_lanthipeptide

Lanthipeptides: EL250_RS01320_lanthipeptide

CYSSCGYYGGSYGGSYGGSYGGSSSSYYGGSYSGGGYYYAAVGYGYSC

97.9%

NZ_CP079819.1and 1 other:

NZ_CP079819.1 Lanthipeptides: LPB401_RS00090_lanthipeptide

Lanthipeptides: LPB401_RS00090_lanthipeptide

CYSSCGYYGGSYGGSYGGSYGGSSSSYYGGSYSGGGYYYAAVGYGYSC

CYSSCGYYGGSYGGSYGGSY GSSSSYYGGSYSGGGYYYAAVGYGYSC

CYSSCGYYGGSYGGSYGGSYCGSSSSYYGGSYSGGGYYYAAVGYGYSC

97.9%

NZ_KI518028.1and 1 other:

NZ_KI518028.1 Lanthipeptides: HMPREF0742_RS01810_lanthipeptide

Lanthipeptides: HMPREF0742_RS01810_lanthipeptide

CYSSCGYYGGSYGGSYGGSYGGSSSSYYGGSYSGGGYYYAAVGYGYSC

CYSSCGYYGGSYGGSYGGSYGGS+SSYYGGSYSGGGYYYAAVGYGYSC

CYSSCGYYGGSYGGSYGGSYGGSNSSYYGGSYSGGGYYYAAVGYGYSC

MIBiG 4.0 matches for EL250_RS01320

8.3%

BGC0000581.6 (AAD28494.1):

Lasso peptide: microcin J25

GSSSSYYG

G+ S+YG

GTPISFYG

EL250_RS01330

Leader:

MPKDITLLEEISDQELGQVNGG

Core:

GSHGGGYYSSVGGGSYGGGYCGGGCGGYGYGFGFWGGSFS

Core with dehydrations:

GDhaHGGGYYDhaDhaVGGGDhaYGGGYCysGGGCysGGYGYGFGFWGGDhaFDha

antiSMASH-DB 4.0 matches for EL250_RS01330

100.0%

NZ_CP068102.1and 7 others:

Lanthipeptides: I6I94_RS04840_lanthipeptide

GSHGGGYYSSVGGGSYGGGYCGGGCGGYGYGFGFWGGSFS

18.2%

NC_014643.1and 16 others:

Lanthipeptides: HMPREF0733_RS05060_lanthipeptide

YGFGFWGGS

+GFGFWGGS

FGFGFWGGS

MIBiG 4.0 matches for EL250_RS01330

15.0%

BGC0002322.3 (CEJ66604.1):

Lasso peptide: citrocin

YCGGGCGGYG

+ GGG G YG

FIGGGVGRYG

allorf_0265595_0265780

Leader:

MTKYCMSHAKYYGA

Core:

ESQKLMFVIYCSPPSARGYTQNARIMSPGVKLWLAGRQPSPVAGCPR

Core with dehydrations:

EDhaQKLMFVIYCysDhaPPDhaARGYDhbQNARIMDhaPGVKLWLAGRQPDhaPVAGCysPR

antiSMASH-DB 4.0 matches for allorf_0265595_0265780

100.0%

NZ_CP079819.1and 5 others:

Lanthipeptides: allorf_0008942_0009127_lanthipeptide

ESQKLMFVIYCSPPSARGYTQNARIMSPGVKLWLAGRQPSPVAGCPR

34.0%

NZ_CP068102.1and 1 other:

NZ_CP068102.1 Lanthipeptides: allorf_1181240_1181359_lanthipeptide

Lanthipeptides: allorf_1181240_1181359_lanthipeptide

ESQKLMFVIYCSPPSARGYT

ESQKLMFVIYCS P RG T

ESQKLMFVIYCS-PHLRGDT

14.9%

NZ_CP054018.1:

Lanthipeptides: allorf_0093622_0093795_lanthipeptide

SQKLMFVIYCSPPSARGYTQNAR

+ L+F + C S RG+ R

NNALIFFVCCRSSSIRGHVSRIR

allorf_0265627_0265695

Leader:

MYPLADGGEQYM

Core:

TNINFCDSAP

Core with dehydrations:

DhbNINFCysDDhaAP

antiSMASH-DB 4.0 matches for allorf_0265627_0265695

100.0%

NZ_CP079819.1and 5 others:

Lanthipeptides: allorf_0008974_0009042_lanthipeptide

TNINFCDSAP

allorf_0265696_0265836

Leader:

MCRWRIRAVLAS

Core:

CSLKPDHHLGHPATGEGCRPASQSFTPGLIILAF

Core with dehydrations:

CysDhaLKPDHHLGHPADhbGEGCysRPADhaQDhaFDhbPGLIILAF

antiSMASH-DB 4.0 matches for allorf_0265696_0265836

100.0%

NZ_CP068102.1and 7 others:

Lanthipeptides: allorf_1181119_1181259_lanthipeptide

CSLKPDHHLGHPATGEGCRPASQSFTPGLIILAF

23.5%

NZ_CP069338.1and 1 other:

NZ_CP069338.1 Lanthipeptides: allorf_09109175_09109414_lanthipeptide

Lanthipeptides: allorf_09109175_09109414_lanthipeptide

HLGHPATGEGCRPASQSFT

G P EG P +SFT

EFGAPPLKEGNHPCLESFT

23.5%

NZ_SNZH01000011.1:

Lanthipeptides: allorf_218796_219029_lanthipeptide

TGEGCRPASQSFT

TG GCR A S T

TGSGCRKAGPSHT

with 1 more sequence matches

14.6%

NZ_LIPB01000002.1:

Lanthipeptides: allorf_1135461_1135619_lanthipeptide

HPATGEGCR

HP G GCR

HPWGGSGCR

MIBiG 4.0 matches for allorf_0265696_0265836

11.8%

BGC0000580.3 (EGD17355.1):

Lasso peptide: xanthomonin II or xanthomonin I

GHPATGE

G P GE

GGPLAGE

8.8%

BGC0000566.5 (SPy_0738):

LAP: streptolysin S

FTPG

+TPG

YTPG

allorf_0265791_0265895

Leader:

MFLHPARRLLIGG

Core:

GLLGVPSCAGGAFAPYLLRVP

Core with dehydrations:

GLLGVPDhaCysAGGAFAPYLLRVP

antiSMASH-DB 4.0 matches for allorf_0265791_0265895

100.0%

NZ_CP068102.1and 5 others:

Lanthipeptides: allorf_1181060_1181164_lanthipeptide

GLLGVPSCAGGAFAPYLLRVP

26.9%

NC_014483.2and 23 others:

Lanthipeptides: PPE_RS07020_lanthipeptide

GLLGVPSCAGGAF

GL+ PSCA G

GLICTPSCATGTL

23.1%

NC_023037.2and 6 others:

Lanthipeptides: X809_RS07820_lanthipeptide

GLLGVPSCAGGAF

G++ PSCA G

GIICTPSCATGTL

with 2 more sequence matches

15.7%

NZ_WWJS01000019.1:

Lanthipeptides: GT018_RS20925_lanthipeptide

GLLGVPSCAGGAFAPY

G VP +GG APY

GCQSVPDQSGGVVAPY

14.3%

NC_017765.1and 1 other:

NZ_KQ948355.1 Lanthipeptides: AQJ11_RS50945_lanthipeptide

Lanthipeptides: SHJG_RS52475_lanthipeptide

VPSCAGGAFAPY

VP+CAGG P+

VPACAGGIARPF

MIBiG 4.0 matches for allorf_0265791_0265895

19.0%

BGC0000503.5 (cinA):

Lanthipeptide: cinnamycin

SCAGGAFA

SC+ G F

SCSFGPFT

allorf_0269996_0270211

Leader:

MEIMMRTYCILPRWFDTVEGLARVAISLLQGRELIVGVLLRMQRMDSIESFRKLILGS

Core:

YCVIGTVLHSHIS

Core with dehydrations:

YCysVIGDhbVLHDhaHIDha

antiSMASH-DB 4.0 matches for allorf_0269996_0270211

100.0%

NZ_CP068102.1and 7 others:

Lanthipeptides: allorf_1176779_1176994_lanthipeptide

YCVIGTVLHSHIS

24.0%

NZ_JACHNX010000043.1:

Lanthipeptides: allorf_0139_0258_lanthipeptide

CVIGTVLHSH

CVIG HS

CVIGEADHSE

10.3%

NZ_CP045142.1:

Lanthipeptides: GBQ70_RS07075_lanthipeptide

YCVIGTVLHSHIS

YC T LH H S

YCDTPTRLHRHES

allorf_0270096_0270281

Leader:

MLCALIALYYG

Core:

HHITKWCDIKWKLRICESVKLFLSHNRSPISAFGSSQCYPFFASSIEHPQ

Core with dehydrations:

HHIDhbKWCysDIKWKLRICysEDhaVKLFLDhaHNRDhaPIDhaAFGDhaDhaQCysYPFFADhaDhaIEHPQ

antiSMASH-DB 4.0 matches for allorf_0270096_0270281

100.0%

NZ_KI518028.1and 3 others:

NZ_KI518028.1 Lanthipeptides: allorf_295332_295517_lanthipeptideNZ_LR134479.1 Lanthipeptides: allorf_0270096_0270281_lanthipeptideNZ_LR134479.1 Lanthipeptides: allorf_0270096_0270281_lanthipeptide

Lanthipeptides: allorf_295332_295517_lanthipeptide

HHITKWCDIKWKLRICESVKLFLSHNRSPISAFGSSQCYPFFASSIEHPQ

98.0%

NZ_CP068102.1and 1 other:

NZ_CP068102.1 Lanthipeptides: allorf_1176709_1176894_lanthipeptide

Lanthipeptides: allorf_1176709_1176894_lanthipeptide

HHITKWCDIKWKLRICESVKLFLSHNRSPISAFGSSQCYPFFASSIEHPQ

HHITKWCDIKWKLRICESVKLFLSHNRSPISAFGSS+CYPFFASSIEHPQ

HHITKWCDIKWKLRICESVKLFLSHNRSPISAFGSSRCYPFFASSIEHPQ

12.0%

NZ_JAMDLZ010000027.1:

Lanthipeptides: M5W82_RS15515_lanthipeptide

KWCDIKWKLRIC

KWC + W+ IC

KWCTLTWECSIC

with 3 more sequence matches

12.0%

NZ_LT745762.1and 1 other:

NZ_LT745762.1 Lanthipeptides: B2C77_RS00795_lanthipeptide

Lanthipeptides: B2C77_RS00795_lanthipeptide

KWCDIKWKLRICES

KWC + W+ IC +

KWCTVTWECSICPT

10.0%

NZ_BQME02000001.1and 1 other:

NZ_BQME02000001.1 Lanthipeptides: MFF31_RS04105_lanthipeptide

Lanthipeptides: MFF31_RS04105_lanthipeptide

KWCDIKWKLRICESVKLF

K+C + W+ +C ++ F

KYCTLTWECSVCPTLTCF

10.0%

NZ_CP013979.1:

Lanthipeptides: ATC03_RS01475_lanthipeptide

NRSPISAFGSSQCYPFFAS

+P+ FG C P+++

GTNPLGTFGCCWCVPWWSG

allorf_0270410_0270481

Leader:

MVENRDLASS

Core:

SPKHSTIKNCSRK

Core with dehydrations:

DhaPKHDhaDhbIKNCysDhaRK

antiSMASH-DB 4.0 matches for allorf_0270410_0270481

100.0%

NZ_LR134479.1and 1 other:

NZ_LR134479.1 Lanthipeptides: allorf_0270410_0270481_lanthipeptide

Lanthipeptides: allorf_0270410_0270481_lanthipeptide

SPKHSTIKNCSRK

92.3%

NZ_CP079819.1and 3 others:

NZ_CP079819.1 Lanthipeptides: allorf_0013756_0013827_lanthipeptideNZ_KI518028.1 Lanthipeptides: allorf_295646_295717_lanthipeptideNZ_KI518028.1 Lanthipeptides: allorf_295646_295717_lanthipeptide

Lanthipeptides: allorf_0013756_0013827_lanthipeptide

SPKHSTIKNCSRK

SPKHS IKNCSRK

SPKHSAIKNCSRK

84.6%

NZ_CP068102.1and 1 other:

NZ_CP068102.1 Lanthipeptides: allorf_1176509_1176580_lanthipeptide

Lanthipeptides: allorf_1176509_1176580_lanthipeptide

SPKHSTIKNCSRK

SPK+S IKNCSRK

SPKYSAIKNCSRK

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lasso peptide predictions

Lassopeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.03 out of 29.72

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 21.39 out of 27.78

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 16.08 out of 18.57

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 24.59 out of 29.72

Confidence: medium

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 20.10 out of 21.84

Confidence: medium

Additional weak hits (12):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.92 out of 27.78

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 22.24 out of 32.38

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 20.68 out of 26.12

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.27 out of 20.01

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.92 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 18.15 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.87 out of 29.38

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 20.75 out of 31.73

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 21.44 out of 36.54

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.97 out of 32.93

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 22.72 out of 32.93

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 21.84 out of 32.38

Confidence: weak

Lanthipeptides

Lasso peptides

RREFinder

TFBS Finder

Core peptide predictions

EL250_RS01320 - Class II

Cleavage pHMM score: 4.70

RODEO score: 0

Monoisotopic mass: 4591.8 Da

Molecular weight: 4594.7 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 4612.7 Da N = 1; 4630.7 Da N = 2; 4648.7 Da N = 3; 4666.7 Da N = 4; 4684.8 Da N = 5; 4702.8 Da N = 6; 4720.8 Da N = 7; 4738.8 Da N = 8; 4756.8 Da N = 9

EL250_RS01330 - Class II

Cleavage pHMM score: 3.20

RODEO score: 0

Monoisotopic mass: 3620.4 Da

Molecular weight: 3622.7 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3640.7 Da N = 1; 3658.8 Da N = 2; 3676.8 Da N = 3; 3694.8 Da N = 4

allorf_0261182_0261298 - Class II

Cleavage pHMM score: -5.20

RODEO score: 0

Monoisotopic mass: 3207.8 Da

Molecular weight: 3209.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3227.7 Da N = 1

allorf_0265595_0265780 - Class II

Cleavage pHMM score: -7.00

RODEO score: 0

Monoisotopic mass: 5038.6 Da

Molecular weight: 5041.9 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 5059.9 Da N = 1; 5077.9 Da N = 2; 5095.9 Da N = 3; 5114.0 Da N = 4

allorf_0265627_0265695 - Class II

Cleavage pHMM score: -7.30

RODEO score: 0

Monoisotopic mass: 1044.5 Da

Molecular weight: 1045.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1063.1 Da N = 1

allorf_0265696_0265836 - Class II

Cleavage pHMM score: -6.10

RODEO score: 0

Monoisotopic mass: 3466.8 Da

Molecular weight: 3468.9 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3487.0 Da N = 1; 3505.0 Da N = 2; 3523.0 Da N = 3

allorf_0265791_0265895 - Class II

Cleavage pHMM score: -6.00

RODEO score: 0

Monoisotopic mass: 2039.1 Da

Molecular weight: 2040.4 Da

Number of bridges: 1

allorf_0269996_0270211 - Class II

Cleavage pHMM score: -4.70

RODEO score: 0

Monoisotopic mass: 1373.7 Da

Molecular weight: 1374.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1392.6 Da N = 1; 1410.6 Da N = 2

allorf_0270096_0270281 - Class II

Cleavage pHMM score: -6.10

RODEO score: 0

Monoisotopic mass: 5695.9 Da

Molecular weight: 5699.5 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 5717.5 Da N = 1; 5735.6 Da N = 2; 5753.6 Da N = 3; 5771.6 Da N = 4; 5789.6 Da N = 5; 5807.6 Da N = 6

allorf_0270410_0270481 - Class II

Cleavage pHMM score: -6.40

RODEO score: 0

Monoisotopic mass: 1412.8 Da

Molecular weight: 1413.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1431.7 Da N = 1; 1449.7 Da N = 2; 1467.7 Da N = 3

EL250_RS01320 - Class II

Cleavage pHMM score: 4.70

RODEO score: 0

Monoisotopic mass: 4591.8 Da

Molecular weight: 4594.7 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 4612.7 Da N = 1; 4630.7 Da N = 2; 4648.7 Da N = 3; 4666.7 Da N = 4; 4684.8 Da N = 5; 4702.8 Da N = 6; 4720.8 Da N = 7; 4738.8 Da N = 8; 4756.8 Da N = 9

EL250_RS01330 - Class II

Cleavage pHMM score: 3.20

RODEO score: 0

Monoisotopic mass: 3620.4 Da

Molecular weight: 3622.7 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3640.7 Da N = 1; 3658.8 Da N = 2; 3676.8 Da N = 3; 3694.8 Da N = 4

allorf_0265595_0265780 - Class II

Cleavage pHMM score: -7.00

RODEO score: 0

Monoisotopic mass: 5038.6 Da

Molecular weight: 5041.9 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 5059.9 Da N = 1; 5077.9 Da N = 2; 5095.9 Da N = 3; 5114.0 Da N = 4

allorf_0265627_0265695 - Class II

Cleavage pHMM score: -7.30

RODEO score: 0

Monoisotopic mass: 1044.5 Da

Molecular weight: 1045.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1063.1 Da N = 1

allorf_0265696_0265836 - Class II

Cleavage pHMM score: -6.10

RODEO score: 0

Monoisotopic mass: 3466.8 Da

Molecular weight: 3468.9 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3487.0 Da N = 1; 3505.0 Da N = 2; 3523.0 Da N = 3

allorf_0265791_0265895 - Class II

Cleavage pHMM score: -6.00

RODEO score: 0

Monoisotopic mass: 2039.1 Da

Molecular weight: 2040.4 Da

Number of bridges: 1

allorf_0269996_0270211 - Class II

Cleavage pHMM score: -4.70

RODEO score: 0

Monoisotopic mass: 1373.7 Da

Molecular weight: 1374.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1392.6 Da N = 1; 1410.6 Da N = 2

allorf_0270096_0270281 - Class II

Cleavage pHMM score: -6.10

RODEO score: 0

Monoisotopic mass: 5695.9 Da

Molecular weight: 5699.5 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 5717.5 Da N = 1; 5735.6 Da N = 2; 5753.6 Da N = 3; 5771.6 Da N = 4; 5789.6 Da N = 5; 5807.6 Da N = 6

allorf_0270410_0270481 - Class II

Cleavage pHMM score: -6.40

RODEO score: 0

Monoisotopic mass: 1412.8 Da

Molecular weight: 1413.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1431.7 Da N = 1; 1449.7 Da N = 2; 1467.7 Da N = 3

Core peptide predictions

No core peptides found.

RRE predictions

: RRE type: Stand_Alone_Lasso_RRE; Location: 14 - 94; Score: 38.9

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
MexT	Global virulence regulator
NtrC	Nitrogen regulatory protein C
ToxT	Regulator of MSHA biosynthesis

NZ_LR134479.1 - Region 2 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 867,370 - 888,503 nt. (total: 21,134 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

EL250_RS03780: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

Transferases: acyltransferase

EL250_RS03795: acyltransferase

smcogs: SMCOG1141: acyltransferase

Ligases: ligase synthetase

EL250_RS03785: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

EL250_RS03815: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002193.2	0.56	other (other)	phenguignardic acid	Aspergillus terreus NIH2624
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000649.3	0.40	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002561.3	0.40	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0000597.4	0.39	ribosomal	SWA-2138	Streptomyces sp. e14
BGC0001910.4	0.39	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000595.3	0.39	ribosomal	SCO-2138	Streptomyces coelicolor A3(2)
BGC0000664.5	0.39	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.38	terpene	isorenieratene	Streptomyces argillaceus
BGC0001911.3	0.38	terpene	raimonol	Streptomyces anulatus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002193.2	0.56	other (other)	phenguignardic acid	Aspergillus terreus NIH2624
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000649.3	0.40	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002561.3	0.40	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0000597.4	0.39	ribosomal	SWA-2138	Streptomyces sp. e14
BGC0001910.4	0.39	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000595.3	0.39	ribosomal	SCO-2138	Streptomyces coelicolor A3(2)
BGC0000664.5	0.39	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.38	terpene	isorenieratene	Streptomyces argillaceus
BGC0001911.3	0.38	terpene	raimonol	Streptomyces anulatus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

EL250_RS03815: PT_FPPS_like

Domain 1 (54 - 372): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 16.04 out of 18.57

Confidence: medium

Additional weak hits (8):

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.03 out of 23.43

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.41 out of 25.75

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 21.87 out of 32.93

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 24.65 out of 39.62

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 18.44 out of 26.06

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 25.59 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 21.44 out of 36.54

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.83 out of 27.78

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
ToxT	Regulator of MSHA biosynthesis

NZ_LR134479.1 - Region 3 - NRPS

Download region GenBank file

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Location: 1,575,724 - 1,619,980 nt. (total: 44,257 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EL250_RS06845: 3-hydroxyisobutyrate dehydrogenase

smcogs: SMCOG1100: 3-hydroxyisobutyrate dehydrogenase

Transferases: formyltransferase transketolase

EL250_RS06855: transketolase

smcogs: SMCOG1204: transketolase

EL250_RS06930: methionyl-tRNA formyltransferase

smcogs: SMCOG1228: methionyl-tRNA formyltransferase

Hydrolases: deformylase esterase

EL250_RS06885: enterobactin/ferric enterobactin esterase

smcogs: SMCOG1098: enterobactin/ferric enterobactin esterase

EL250_RS06935: peptide deformylase

smcogs: SMCOG1275: peptide deformylase

Ligases: ligase synthase

EL250_RS06830: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

EL250_RS06880: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002685.2	0.79	NRPS	enterobactin	Rothia mucilaginosa ATCC 25296
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001132.4	0.66	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.63	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.63	NRPS	AM-toxin	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002685.2	0.79	NRPS	enterobactin	Rothia mucilaginosa ATCC 25296
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001132.4	0.66	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.63	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.63	NRPS	AM-toxin	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.19 out of 20.47

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.91 out of 23.79

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 23.16 out of 30.25

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 21.40 out of 23.05

Confidence: strong

Additional weak hits (6):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.47 out of 29.38

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.95 out of 39.62

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.00 out of 26.06

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.22 out of 25.75

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.90 out of 30.98

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 19.56 out of 28.14

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 5, location 1575723 - 1619980:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

EL250_RS06870: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (515..966): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWQTFDVHVWEWHFVCSGELNLYGPTEAAVDVTF
Nearest Stachelhaus code(s):: Ser DVWHVSLVDK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

EL250_RS06880: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (50..465): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: SGSLFTMSSPGGALQVGGAQQVFGMAEGLVNYTR
Nearest Stachelhaus code(s):: diOH-Bz TMPSQGVVNK (94% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
ColR	Phenol-responsive regulator
IolR	Regulator of the inositol catabolism
VqsM	Regulator of quorum-sensing signalling systems