GCF_027671065.1 - 3 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_JAQEAZ010000001.1 (Blautia hansenii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	terpene-precursor	176,672	197,562

NZ_JAQEAZ010000003.1 (Blautia hansenii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 23.1	ranthipeptide	78,755	100,467

NZ_JAQEAZ010000004.1 (Blautia hansenii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 31.1	lanthipeptide-class-i,cyclic-lactone-autoinducer	1	24,853	Low	nisin O	ribosomal:RiPP:Lanthipeptide

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	terpene-precursor	176,672	197,562
Region 23.1	ranthipeptide	78,755	100,467
Region 31.1	lanthipeptide-class-i,cyclic-lactone-autoinducer	1	24,853	Low	nisin O	ribosomal:RiPP:Lanthipeptide

No secondary metabolite regions were found in these records:: NZ_JAQEAZ010000010.1 (Blautia hansenii); NZ_JAQEAZ010000011.1 (Blautia hansenii); NZ_JAQEAZ010000012.1 (Blautia hansenii); NZ_JAQEAZ010000013.1 (Blautia hansenii); NZ_JAQEAZ010000014.1 (Blautia hansenii); NZ_JAQEAZ010000015.1 (Blautia hansenii); NZ_JAQEAZ010000016.1 (Blautia hansenii); NZ_JAQEAZ010000017.1 (Blautia hansenii); NZ_JAQEAZ010000018.1 (Blautia hansenii); NZ_JAQEAZ010000019.1 (Blautia hansenii); NZ_JAQEAZ010000002.1 (Blautia hansenii); NZ_JAQEAZ010000020.1 (Blautia hansenii); NZ_JAQEAZ010000021.1 (Blautia hansenii); NZ_JAQEAZ010000022.1 (Blautia hansenii); NZ_JAQEAZ010000023.1 (Blautia hansenii); NZ_JAQEAZ010000024.1 (Blautia hansenii); NZ_JAQEAZ010000025.1 (Blautia hansenii); NZ_JAQEAZ010000026.1 (Blautia hansenii); NZ_JAQEAZ010000027.1 (Blautia hansenii); NZ_JAQEAZ010000028.1 (Blautia hansenii); NZ_JAQEAZ010000029.1 (Blautia hansenii); NZ_JAQEAZ010000030.1 (Blautia hansenii); NZ_JAQEAZ010000031.1 (Blautia hansenii); NZ_JAQEAZ010000032.1 (Blautia hansenii); NZ_JAQEAZ010000033.1 (Blautia hansenii); NZ_JAQEAZ010000034.1 (Blautia hansenii); NZ_JAQEAZ010000035.1 (Blautia hansenii); NZ_JAQEAZ010000036.1 (Blautia hansenii); NZ_JAQEAZ010000005.1 (Blautia hansenii); NZ_JAQEAZ010000006.1 (Blautia hansenii); NZ_JAQEAZ010000007.1 (Blautia hansenii); NZ_JAQEAZ010000008.1 (Blautia hansenii); NZ_JAQEAZ010000009.1 (Blautia hansenii)

Compact view

NZ_JAQEAZ010000001.1 - Region 1 - terpene-precursor

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Location: 176,672 - 197,562 nt. (total: 20,891 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: glycosyltransferase

PGT18_RS01000: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

Hydrolases: back translocase

PGT18_RS00935: GTP-binding protein LepA

smcogs: SMCOG1190: GTP-binding protein LepA

Ligases: synthase synthetase

PGT18_RS00960: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

PGT18_RS00965: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0000665.5	0.38	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0001456.3	0.38	terpene	isorenieratene	Streptomyces argillaceus
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0001605.3	0.37	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0000633.3	0.37	terpene	carotenoid	Streptomyces avermitilis
BGC0001604.3	0.36	terpene	gibberellin	Fusarium fujikuroi

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0000665.5	0.38	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0001456.3	0.38	terpene	isorenieratene	Streptomyces argillaceus
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0001605.3	0.37	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0000633.3	0.37	terpene	carotenoid	Streptomyces avermitilis
BGC0001604.3	0.36	terpene	gibberellin	Fusarium fujikuroi

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

PGT18_RS00960: PT_FPPS_like

Domain 1 (32 - 295): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: DMAPP, IPP → FPP

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 23.16 out of 29.25

Confidence: medium

Additional weak hits (1):

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 24.65 out of 33.14

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
SypG	Regulator of biofilm formation and host colonization
ToxT	Regulator of MSHA biosynthesis

NZ_JAQEAZ010000003.1 - Region 1 - ranthipeptide

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Location: 78,755 - 100,467 nt. (total: 21,713 nt) Show pHMM detection rules used

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

PGT18_RS05195: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002306.2	0.47	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0002138.2	0.37	PKS	fuelimycin A, fuelimicin B, fuelimicin C	Streptomyces albireticuli NRRL B-1670
BGC0001387.5	0.33	other (other)	nucleocidin	Streptomyces calvus
BGC0000613.5	0.32	ribosomal	thiomuracin A	Nonomuraea sp. Bp3714-39
BGC0001860.4	0.32	ribosomal	thiomuracin	Thermobispora bispora DSM 43833
BGC0001155.5	0.31	ribosomal	GE2270	Planobispora rosea
BGC0000604.4	0.31	ribosomal	GE2270A	Nonomuraea sp. WU8817
BGC0001002.5	0.30	NRPS, PKS	jawsamycin	Streptomyces roseoverticillatus
BGC0002713.2	0.30	NRPS	mevalagmapeptide A, mevalagmapeptide B, mevalagmapeptide C, mevalagmapeptide D	Photorhabdus laumondii subsp. laumondii
BGC0001591.4	0.29	other (other)	fatty acid enol ester, N-tetradecanoyl tyrosine	uncultured bacterium CSLC2

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002306.2	0.47	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0002138.2	0.37	PKS	fuelimycin A, fuelimicin B, fuelimicin C	Streptomyces albireticuli NRRL B-1670
BGC0001387.5	0.33	other (other)	nucleocidin	Streptomyces calvus
BGC0000613.5	0.32	ribosomal	thiomuracin A	Nonomuraea sp. Bp3714-39
BGC0001860.4	0.32	ribosomal	thiomuracin	Thermobispora bispora DSM 43833
BGC0001155.5	0.31	ribosomal	GE2270	Planobispora rosea
BGC0000604.4	0.31	ribosomal	GE2270A	Nonomuraea sp. WU8817
BGC0001002.5	0.30	NRPS, PKS	jawsamycin	Streptomyces roseoverticillatus
BGC0002713.2	0.30	NRPS	mevalagmapeptide A, mevalagmapeptide B, mevalagmapeptide C, mevalagmapeptide D	Photorhabdus laumondii subsp. laumondii
BGC0001591.4	0.29	other (other)	fatty acid enol ester, N-tetradecanoyl tyrosine	uncultured bacterium CSLC2

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.62 out of 19.52

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.07 out of 18.46

Confidence: medium

Additional weak hits (5):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 24.82 out of 39.62

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 20.88 out of 26.12

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.59 out of 27.18

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.37 out of 30.98

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 23.65 out of 30.98

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: Ranthipeptide_rSAM_RRE; Location: 0 - 99; Score: 111.4

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
RpoN	Regulator of nitrogen assimilation and virulence

NZ_JAQEAZ010000004.1 - Region 1 - cyclic-lactone-autoinducer,lanthipeptide-class-i

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Location: 1 - 24,853 nt. (total: 24,853 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))
cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase dehydratase

PGT18_RS05880: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

PGT18_RS05890: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000538.3	0.44	ribosomal	nisin Z	Lactococcus lactis
BGC0001289.5	0.43	ribosomal	penisin	Paenibacillus ehimensis
BGC0002576.2	0.43	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0001701.4	0.42	ribosomal	nisin O	Blautia obeum
BGC0000515.4	0.37	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0002328.3	0.37	ribosomal	Rc-AIP1	Ruminiclostridium cellulolyticum H10
BGC0000506.4	0.36	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000542.3	0.34	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000541.5	0.34	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000559.4	0.34	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.74	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0001701.4	0.64	ribosomal	nisin O	Blautia obeum
BGC0000538.3	0.62	ribosomal	nisin Z	Lactococcus lactis
BGC0001289.5	0.59	ribosomal	penisin	Paenibacillus ehimensis
BGC0000542.3	0.56	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000541.5	0.56	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000515.4	0.56	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000506.4	0.54	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000559.4	0.54	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0000556.5	0.53	ribosomal	streptin	Streptococcus pyogenes

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for PGT18_RS05880

PGT18_RS05860

Leader:

MAKFDDFDLDIIEKKVRRDIILASI

Core:

TSQHSFCTPNCPTGFLCPPKTQLTCTCKLKGR

Core with dehydrations:

DhbDhaQHDhaFCysDhbPNCysPDhbGFLCysPPKDhbQLDhbCysDhbCysKLKGR

antiSMASH-DB 4.0 matches for PGT18_RS05860

100.0%

NZ_JAQEAZ010000004.1:

Lanthipeptides: PGT18_RS05860_lanthipeptide

TSQHSFCTPNCPTGFLCPPKTQLTCTCKLKGR

88.2%

NC_021022.1and 2 others:

NC_021022.1 Lanthipeptides: CK5_RS17320_lanthipeptideNZ_JADNJR010000016.1 Lanthipeptides: I2E58_RS10870_lanthipeptide

Lanthipeptides: CK5_RS17320_lanthipeptide

TSQHSFCTPNCPTGFLCPPKTQLTCTCKLKGR

TSQHSFCTPNC TGFLCPPKTQLTCTCKLKG+

TSQHSFCTPNCLTGFLCPPKTQLTCTCKLKGQ

51.5%

NZ_JADNJR010000016.1and 2 others:

NZ_JADNJR010000016.1 Lanthipeptides: I2E58_RS10875_lanthipeptideNZ_QSHK01000002.1 Lanthipeptides: DW860_RS04740_lanthipeptide

Lanthipeptides: I2E58_RS10880_lanthipeptide

SFCTPNCPTGFL--CPPKTQLTCTCKLKGR

S CTP CPTG L CP KT TC C + G+

SACTPGCPTGILMTCPLKTA-TCGCHITGK

with 53 more sequence matches

Only the first 10 are displayed here.

51.5%

NZ_QSHK01000002.1and 1 other:

NZ_QSHK01000002.1 Lanthipeptides: DW860_RS04730_lanthipeptide

Lanthipeptides: DW860_RS04735_lanthipeptide

SFCTPNCPTGFL--CPPKTQLTCTCKLKGR

S CTP CPTG L CP KT TC C + G+

SVCTPGCPTGILMTCPLKTA-TCGCHITGK

46.9%

NC_014479.1and 9 others:

NZ_CP026362.1 Lanthipeptides: BV11031_RS00400_lanthipeptideNZ_CP033052.1 Lanthipeptides: D9779_RS17315_lanthipeptideNZ_CP051464.1 Lanthipeptides: HC660_RS17030_lanthipeptideNZ_JALAKK010000002.1 Lanthipeptides: MOB09_RS02800_lanthipeptideNZ_JALAKO010000014.1 Lanthipeptides: MOB26_RS08535_lanthipeptideNZ_JALAOG010000003.1 Lanthipeptides: MOC96_RS02500_lanthipeptideNZ_JALAPG010000043.1 Lanthipeptides: MOC70_RS16815_lanthipeptideNZ_JALAQI010000009.1 Lanthipeptides: MOD13_RS06065_lanthipeptideNZ_NXEM01000027.1 Lanthipeptides: CPT06_RS15285_lanthipeptide

Lanthipeptides: BSUW23_RS16845_lanthipeptide

HSFCTPNCPTGFL--CPPKTQLTCTCKL

S CTP C TG L C +T LTC CK+

ESLCTPGCVTGALQTCFLQT-LTCNCKI

46.9%

NZ_CP059471.1and 3 others:

NZ_FNZW01000001.1 Lanthipeptides: BMZ61_RS02110_lanthipeptideNZ_FOYC01000001.1 Lanthipeptides: BMZ88_RS03370_lanthipeptideNZ_KE384124.1 Lanthipeptides: G597_RS0106700_lanthipeptide

Lanthipeptides: H1R75_RS06635_lanthipeptide

SQHSFCTPNCPTGFL--CPPKTQLTCTCKL

+ S CTP C TG L CP KT TC C

TSKSLCTPGCKTGALMTCPIKTA-TCGCHF

45.5%

NZ_CP015405.2and 7 others:

NZ_CP015405.2 Lanthipeptides: A4V09_RS08295_lanthipeptideNZ_CP015405.2 Lanthipeptides: A4V09_RS08290_lanthipeptideNZ_CP015405.2 Lanthipeptides: A4V09_RS08285_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06240_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06235_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06230_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06225_lanthipeptide

Lanthipeptides: A4V09_RS08300_lanthipeptide

SQHSFCTPNCPTGFL--CPPKTQLTCTCKLKGR

+ S CTP C TG L CP +T TC C++ G+

TSKSLCTPGCVTGILMTCPVQTA-TCGCQITGK

43.8%

NC_015600.1and 14 others:

NZ_AP020310.1 Lanthipeptides: SAGCMC97051_RS07300_lanthipeptideNZ_ATVP01000001.1 Lanthipeptides: G574_RS11150_lanthipeptideNZ_BCFB01000042.1 Lanthipeptides: GOZ36_RS07980_lanthipeptideNZ_CP068708.1 Lanthipeptides: JM964_RS04040_lanthipeptideNZ_CP116958.1 Lanthipeptides: M0P28_RS07755_lanthipeptideNZ_CP116958.1 Lanthipeptides: M0P28_RS07755_lanthipeptideNZ_FIKS01000005.1 Lanthipeptides: A0525_RS10015_lanthipeptideNZ_GL636070.1 Lanthipeptides: HMPREF9171_RS106200_lanthipeptideNZ_JADPLU010000018.1 Lanthipeptides: I4V82_RS09545_lanthipeptideNZ_JAKVIE010000017.1 Lanthipeptides: MK857_RS08340_lanthipeptideNZ_JAQDQV010000001.1 Lanthipeptides: PGO74_RS00635_lanthipeptideNZ_JAQDQV010000001.1 Lanthipeptides: PGO74_RS00635_lanthipeptideNZ_LR594050.1 Lanthipeptides: FGL20_RS03485_lanthipeptideNZ_SSXN01000004.1 Lanthipeptides: FAJ36_RS04715_lanthipeptide

Lanthipeptides: SGPB_RS10680_lanthipeptide

TSQHSFCTPNCPTGFL--CPPKTQLTCTC

+ S CTP C TG L C KT TC C

VTSKSLCTPGCKTGILMTCAIKTA-TCGC

43.8%

NC_016047.1and 2 others:

NZ_KZ848487.1 Lanthipeptides: DSL71_RS15965_lanthipeptideNZ_MUXL01000003.1 Lanthipeptides: B0W20_RS12275_lanthipeptide

Lanthipeptides: GYO_RS39160_lanthipeptide

HSFCTPNCPTGFL--CPPKTQLTCTCKL

S CTP C TG L C +T +TC CK+

ESVCTPGCVTGLLQTCFLQT-ITCNCKI

43.8%

NZ_CP021903.1and 20 others:

NZ_CP023409.1 Lanthipeptides: Bateq7PJ16_RS18530_lanthipeptideNZ_CP026036.1 Lanthipeptides: C2H93_RS15000_lanthipeptideNZ_CP028218.1 Lanthipeptides: C7M30_RS14555_lanthipeptideNZ_CP035167.1 Lanthipeptides: EQJ08_RS17140_lanthipeptideNZ_CP035226.1 Lanthipeptides: EQW70_RS18175_lanthipeptideNZ_CP045006.1 Lanthipeptides: F9B13_RS03875_lanthipeptideNZ_CP076083.1 Lanthipeptides: KFF76_RS16710_lanthipeptideNZ_CP079759.1 Lanthipeptides: KXZ66_RS17780_lanthipeptideNZ_CP080508.1 Lanthipeptides: K0V03_RS06525_lanthipeptideNZ_CP089148.1 Lanthipeptides: LRO88_RS19055_lanthipeptideNZ_JALAUA010000003.1 Lanthipeptides: MOD32_RS04985_lanthipeptideNZ_JALAVX010000009.1 Lanthipeptides: MOE40_RS09385_lanthipeptideNZ_JAMYDW010000007.1 Lanthipeptides: NHM07_RS16180_lanthipeptideNZ_JAQOXM010000001.1 Lanthipeptides: PO770_RS03875_lanthipeptideNZ_NJRD01000003.1 Lanthipeptides: CE053_RS07200_lanthipeptideNZ_NJRE01000008.1 Lanthipeptides: CE047_RS18435_lanthipeptideNZ_SORJ01000001.1 Lanthipeptides: BJ795_RS01490_lanthipeptideNZ_VFNF01000001.1 Lanthipeptides: FB592_RS01525_lanthipeptideNZ_VIOH01000001.1 Lanthipeptides: FLN47_RS03850_lanthipeptideNZ_VLJL01000012.1 Lanthipeptides: L604_RS09425_lanthipeptide

Lanthipeptides: CD007_RS17065_lanthipeptide

HSFCTPNCPTGFL--CPPKTQLTCTCKL

S CTP C TG L C +T +TC CK+

ESLCTPGCVTGVLQTCFLQT-ITCNCKI

43.8%

NZ_CP024974.1and 1 other:

NZ_JAIMDQ010000006.1 Lanthipeptides: K6V44_RS04045_lanthipeptide

Lanthipeptides: CVO91_RS06555_lanthipeptide

SFCTPNCPTGFL--CPPKTQLTCTC

S CTP C TG L C KT TC C

SLCTPGCKTGILMTCAIKTA-TCGC

41.2%

NC_013656.1and 20 others:

NC_020450.1 Lanthipeptides: LILO_RS03015_lanthipeptideNZ_CP009472.1 Lanthipeptides: LG36_RS03420_lanthipeptideNZ_CP010050.1 Lanthipeptides: QI18_RS03300_lanthipeptideNZ_CP015903.1 Lanthipeptides: LLUC08_RS03255_lanthipeptideNZ_CP024954.1 Lanthipeptides: CV702_RS02990_lanthipeptideNZ_CP066300.1 Lanthipeptides: LacL0098_RS03205_lanthipeptideNZ_CP098790.1 Lanthipeptides: M5C73_RS03405_lanthipeptideNZ_JAALEQ010000006.1 Lanthipeptides: HFD15_RS10120_lanthipeptideNZ_JAJONJ010000005.1 Lanthipeptides: LSG16_RS07195_lanthipeptideNZ_JAQDWM010000007.1 Lanthipeptides: PGU01_RS08180_lanthipeptideNZ_JAQDXV010000018.1 Lanthipeptides: PGT80_RS11850_lanthipeptideNZ_JAQDYA010000007.1 Lanthipeptides: PGT72_RS09560_lanthipeptideNZ_JAQDYA010000007.1 Lanthipeptides: PGT72_RS09560_lanthipeptideNZ_JAQEIZ010000019.1 Lanthipeptides: PGX18_RS12025_lanthipeptideNZ_JAQEOU010000022.1 Lanthipeptides: PGV42_RS12330_lanthipeptideNZ_JAQEOV010000015.1 Lanthipeptides: PGW09_RS10765_lanthipeptideNZ_MTJP01000006.1 Lanthipeptides: BW151_RS03795_lanthipeptideNZ_MTJR01000005.1 Lanthipeptides: BW153_RS02980_lanthipeptideNZ_MTJS01000002.1 Lanthipeptides: BW154_RS09850_lanthipeptideNZ_RAGL01000022.1 Lanthipeptides: D6108_RS11395_lanthipeptide

Lanthipeptides: LLKF_RS13210_lanthipeptide

SFCTPNCPTGFL--CPPKTQLTCTCKL

S CTP C TG L C KT TC C +

SLCTPGCKTGALMGCNMKTA-TCNCSI

41.2%

NZ_CP065984.1and 2 others:

NZ_CP095737.1 Lanthipeptides: MXM90_RS03205_lanthipeptideNZ_JAQDXJ010000021.1 Lanthipeptides: PGU19_RS11740_lanthipeptide

Lanthipeptides: I6I21_RS02785_lanthipeptide

SFCTPNCPTGFL--CPPKTQLTCTCKL

S CTP C TG L C KT TC C +

SLCTPGCKTGALMGCNMKTA-TCHCSI

MIBiG 4.0 matches for PGT18_RS05860

41.2%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

SFCTPNCPTGFL--CPPKTQLTCTCKL

S CTP C TG L C KT TC C +

SLCTPGCKTGALMGCNMKTA-TCHCSI

15.6%

BGC0001155.5 (AGY49587.1):

Thiopeptide: GE2270

NCPTGFLC

NC GF C

NCVCGFCC

12.5%

BGC0000582.4 (ADR72962.1):

Linaridin: cypemycin

TPNCPT

TP PT

TPATPT

with 1 more sequence matches

10.3%

BGC0001229.4 (SBI_06990)and 1 other:

BGC0002110.3 (QKV49893.1) Lanthipeptide: birimositide

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

CTPNC

C PNC

CQPNC

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.57 out of 18.57

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.15 out of 27.78

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.28 out of 23.05

Confidence: medium

Additional weak hits (2):

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 22.23 out of 33.73

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.70 out of 27.18

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

PGT18_RS05860 - Class I

Cleavage pHMM score: -0.20

RODEO score: 14

Monoisotopic mass: 3352.7 Da

Molecular weight: 3354.9 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 3373.0 Da N = 1; 3391.0 Da N = 2; 3409.0 Da N = 3

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 142 - 228; Score: 45.4

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
ToxT	Regulator of MSHA biosynthesis