GCF_023469985.1 - 2 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_JAMDIB010000003.1 (Streptococcus suis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 29.1	T3PKS,terpene-precursor	164,124	205,278

NZ_JAMDIB010000004.1 (Streptococcus suis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 30.1	lanthipeptide-class-i	47,067	73,101	High	suicin 90-1330	ribosomal:RiPP

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 29.1	T3PKS,terpene-precursor	164,124	205,278
Region 30.1	lanthipeptide-class-i	47,067	73,101	High	suicin 90-1330	ribosomal:RiPP

No secondary metabolite regions were found in these records:: NZ_JAMDIB010000010.1 (Streptococcus suis); NZ_JAMDIB010000011.1 (Streptococcus suis); NZ_JAMDIB010000012.1 (Streptococcus suis); NZ_JAMDIB010000013.1 (Streptococcus suis); NZ_JAMDIB010000014.1 (Streptococcus suis); NZ_JAMDIB010000015.1 (Streptococcus suis); NZ_JAMDIB010000016.1 (Streptococcus suis); NZ_JAMDIB010000017.1 (Streptococcus suis); NZ_JAMDIB010000018.1 (Streptococcus suis); NZ_JAMDIB010000019.1 (Streptococcus suis); NZ_JAMDIB010000001.1 (Streptococcus suis); NZ_JAMDIB010000020.1 (Streptococcus suis); NZ_JAMDIB010000021.1 (Streptococcus suis); NZ_JAMDIB010000022.1 (Streptococcus suis); NZ_JAMDIB010000023.1 (Streptococcus suis); NZ_JAMDIB010000024.1 (Streptococcus suis); NZ_JAMDIB010000025.1 (Streptococcus suis); NZ_JAMDIB010000026.1 (Streptococcus suis); NZ_JAMDIB010000027.1 (Streptococcus suis); NZ_JAMDIB010000028.1 (Streptococcus suis); NZ_JAMDIB010000029.1 (Streptococcus suis); NZ_JAMDIB010000002.1 (Streptococcus suis); NZ_JAMDIB010000030.1 (Streptococcus suis); NZ_JAMDIB010000031.1 (Streptococcus suis); NZ_JAMDIB010000032.1 (Streptococcus suis); NZ_JAMDIB010000033.1 (Streptococcus suis); NZ_JAMDIB010000034.1 (Streptococcus suis); NZ_JAMDIB010000035.1 (Streptococcus suis); NZ_JAMDIB010000005.1 (Streptococcus suis); NZ_JAMDIB010000006.1 (Streptococcus suis); NZ_JAMDIB010000007.1 (Streptococcus suis); NZ_JAMDIB010000008.1 (Streptococcus suis); NZ_JAMDIB010000009.1 (Streptococcus suis)

Compact view

NZ_JAMDIB010000003.1 - Region 1 - T3PKS,terpene-precursor

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Location: 164,124 - 205,278 nt. (total: 41,155 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase reductase

M5P35_RS03515: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

M5P35_RS03550: hydroxymethylglutaryl-CoA reductase

smcogs: SMCOG1187: hydroxymethylglutaryl-CoA reductase

Transferases: acetyltransferase methyltransferase

M5P35_RS03445: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

M5P35_RS03540: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

M5P35_RS03615: isoprenylcysteine carboxyl methyltransferase

smcogs: SMCOG1150: isoprenylcysteine carboxyl methyltransferase

Hydrolases: deacetylase peptidase

M5P35_RS03440: methionine aminopeptidase

smcogs: SMCOG1172: methionine aminopeptidase

M5P35_RS03525: polysaccharide deacetylase

smcogs: SMCOG1235: polysaccharide deacetylase

Isomerases: mutase

M5P35_RS03535: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: synthase synthetase

M5P35_RS03545: hydroxymethylglutaryl-CoA synthase

smcogs: SMCOG1043: hydroxymethylglutaryl-CoA synthase

M5P35_RS03595: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.43	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.37	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.29	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000205.3	0.29	PKS	bryostatin	Candidatus Endobugula sertula
BGC0002561.3	0.27	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0001605.3	0.26	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0000286.3	0.24	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0001456.3	0.24	terpene	isorenieratene	Streptomyces argillaceus
BGC0000550.3	0.24	ribosomal	salivaricin G32	Streptococcus salivarius
BGC0000665.5	0.23	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.54	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.51	terpene	boleracene	Brassica oleracea var. oleracea
BGC0000866.5	0.48	other (other)	polyhydroxyalkanoate	Burkholderia sp. DSM 9242
BGC0000867.3	0.46	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0002398.2	0.44	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000205.3	0.44	PKS	bryostatin	Candidatus Endobugula sertula
BGC0000278.3	0.42	PKS	urdamycin	Streptomyces fradiae
BGC0001605.3	0.42	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0002561.3	0.42	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0001595.3	0.41	other (other)	fischerindole	Fischerella sp. SAG 46.79

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

M5P35_RS03595: PT_FPPS_like

Domain 1 (25 - 281): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: IPP, DMAPP → FPP

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.45 out of 26.06

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.26 out of 27.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.64 out of 26.06

Confidence: medium

Additional weak hits (3):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.22 out of 30.25

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 24.36 out of 36.54

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.42 out of 29.38

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
MogR	Repressor of flagellar motility genes

NZ_JAMDIB010000004.1 - Region 1 - lanthipeptide-class-i

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Location: 47,067 - 73,101 nt. (total: 26,035 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: esterase protease

M5P35_RS03960: putative esterase

smcogs: SMCOG1104: putative esterase

M5P35_RS04060: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: cyclase dehydratase

M5P35_RS04015: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

M5P35_RS04025: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001789.3	0.97	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000537.3	0.85	ribosomal	nisin U	Streptococcus uberis
BGC0000536.3	0.76	ribosomal	nisin Q	Lactococcus lactis
BGC0000535.4	0.74	ribosomal	nisin A	Lactococcus lactis subsp. lactis
BGC0000538.3	0.72	ribosomal	nisin Z	Lactococcus lactis
BGC0000549.3	0.58	ribosomal	salivaricin D	Streptococcus salivarius
BGC0000506.4	0.57	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000559.4	0.55	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0000556.5	0.55	ribosomal	streptin	Streptococcus pyogenes
BGC0001618.3	0.54	ribosomal	hyicin 3682	Staphylococcus hyicus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001789.3	0.97	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000537.3	0.85	ribosomal	nisin U	Streptococcus uberis
BGC0000536.3	0.76	ribosomal	nisin Q	Lactococcus lactis
BGC0000535.4	0.74	ribosomal	nisin A	Lactococcus lactis subsp. lactis
BGC0000538.3	0.72	ribosomal	nisin Z	Lactococcus lactis
BGC0000549.3	0.58	ribosomal	salivaricin D	Streptococcus salivarius
BGC0000506.4	0.57	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000559.4	0.55	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0000556.5	0.55	ribosomal	streptin	Streptococcus pyogenes
BGC0001618.3	0.54	ribosomal	hyicin 3682	Staphylococcus hyicus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for M5P35_RS04025

M5P35_RS04030

Leader:

MNNEDFNLDLVTISKENNSGASPR

Core:

VTSKSLCTPPGCKTGILMTCAIKTATCGCHFG

Core with dehydrations:

VDhbDhaKDhaLCysDhbPPGCysKDhbGILMDhbCysAIKDhbADhbCysGCysHFG

antiSMASH-DB 4.0 matches for M5P35_RS04030

100.0%

NZ_JAMDIB010000004.1:

Lanthipeptides: M5P35_RS04030_lanthipeptide

VTSKSLCTPPGCKTGILMTCAIKTATCGCHFG

96.9%

NC_015600.1and 14 others:

NZ_AP020310.1 Lanthipeptides: SAGCMC97051_RS07300_lanthipeptideNZ_ATVP01000001.1 Lanthipeptides: G574_RS11150_lanthipeptideNZ_BCFB01000042.1 Lanthipeptides: GOZ36_RS07980_lanthipeptideNZ_CP068708.1 Lanthipeptides: JM964_RS04040_lanthipeptideNZ_CP116958.1 Lanthipeptides: M0P28_RS07755_lanthipeptideNZ_CP116958.1 Lanthipeptides: M0P28_RS07755_lanthipeptideNZ_FIKS01000005.1 Lanthipeptides: A0525_RS10015_lanthipeptideNZ_GL636070.1 Lanthipeptides: HMPREF9171_RS106200_lanthipeptideNZ_JADPLU010000018.1 Lanthipeptides: I4V82_RS09545_lanthipeptideNZ_JAKVIE010000017.1 Lanthipeptides: MK857_RS08340_lanthipeptideNZ_JAQDQV010000001.1 Lanthipeptides: PGO74_RS00635_lanthipeptideNZ_JAQDQV010000001.1 Lanthipeptides: PGO74_RS00635_lanthipeptideNZ_LR594050.1 Lanthipeptides: FGL20_RS03485_lanthipeptideNZ_SSXN01000004.1 Lanthipeptides: FAJ36_RS04715_lanthipeptide

Lanthipeptides: SGPB_RS10680_lanthipeptide

VTSKSLCTPPGCKTGILMTCAIKTATCGCHFG

VTSKSLCTP GCKTGILMTCAIKTATCGCHFG

VTSKSLCTP-GCKTGILMTCAIKTATCGCHFG

90.6%

NZ_CP024974.1and 1 other:

NZ_JAIMDQ010000006.1 Lanthipeptides: K6V44_RS04045_lanthipeptide

Lanthipeptides: CVO91_RS06555_lanthipeptide

VTSKSLCTPPGCKTGILMTCAIKTATCGCHF

VTS SLCTP GCKTGILMTCAIKTATCGCHF

VTSISLCTP-GCKTGILMTCAIKTATCGCHF

with 64 more sequence matches

Only the first 10 are displayed here.

87.5%

NZ_CP059471.1and 3 others:

NZ_FNZW01000001.1 Lanthipeptides: BMZ61_RS02110_lanthipeptideNZ_FOYC01000001.1 Lanthipeptides: BMZ88_RS03370_lanthipeptideNZ_KE384124.1 Lanthipeptides: G597_RS0106700_lanthipeptide

Lanthipeptides: H1R75_RS06635_lanthipeptide

VTSKSLCTPPGCKTGILMTCAIKTATCGCHFG

+TSKSLCTP GCKTG LMTC IKTATCGCHFG

ITSKSLCTP-GCKTGALMTCPIKTATCGCHFG

77.8%

NZ_NHVL01000013.1and 1 other:

NZ_NHVL01000013.1 Lanthipeptides: CEU70_RS16830_lanthipeptide

Lanthipeptides: CEU70_RS16825_lanthipeptide

VTSKSLCTPPGCKTGILMTCAIKTATCGC--HFG

VTSKSLCTP GCKTGIL TCAIK+ATCGC H G

VTSKSLCTP-GCKTGILQTCAIKSATCGCSIHIG

69.7%

NZ_CP015405.2and 7 others:

NZ_CP015405.2 Lanthipeptides: A4V09_RS08295_lanthipeptideNZ_CP015405.2 Lanthipeptides: A4V09_RS08290_lanthipeptideNZ_CP015405.2 Lanthipeptides: A4V09_RS08285_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06240_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06235_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06230_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06225_lanthipeptide

Lanthipeptides: A4V09_RS08300_lanthipeptide

VTSKSLCTPPGCKTGILMTCAIKTATCGCHF

+TSKSLCTP GC TGILMTC ++TATCGC

ITSKSLCTP-GCVTGILMTCPVQTATCGCQI

69.7%

NZ_JADNJR010000016.1and 2 others:

NZ_JADNJR010000016.1 Lanthipeptides: I2E58_RS10875_lanthipeptideNZ_QSHK01000002.1 Lanthipeptides: DW860_RS04740_lanthipeptide

Lanthipeptides: I2E58_RS10880_lanthipeptide

SKSLCTPPGCKTGILMTCAIKTATCGCHF

SKS CTP GC TGILMTC +KTATCGCH

SKSACTP-GCPTGILMTCPLKTATCGCHI

69.7%

NZ_QSHK01000002.1and 1 other:

NZ_QSHK01000002.1 Lanthipeptides: DW860_RS04730_lanthipeptide

Lanthipeptides: DW860_RS04735_lanthipeptide

SKSLCTPPGCKTGILMTCAIKTATCGCHF

SKS+CTP GC TGILMTC +KTATCGCH

SKSVCTP-GCPTGILMTCPLKTATCGCHI

65.7%

NZ_FNDP01000013.1:

Lanthipeptides: BLP54_RS11230_lanthipeptide

VTSKSLCTPPGCKTGILMTCAIKTATCGC--HFG

+TSKSLCTP GC TG+LM C KTATC C H G

ITSKSLCTP-GCVTGVLMGCNNKTATCNCSVHVG

64.7%

NZ_CP090412.1and 3 others:

NZ_QSTB01000004.1 Lanthipeptides: DXC22_RS03785_lanthipeptideNZ_VSTZ01000009.1 Lanthipeptides: FYK98_RS07445_lanthipeptideNZ_VSTZ01000009.1 Lanthipeptides: FYK98_RS07445_lanthipeptide

Lanthipeptides: LZF92_RS09890_lanthipeptide

VTSKSLCTPPGCKTGILMTCAIKTATCGCHF

+TS SLCTP GCKTG LM C +KTA+CGCH

ITSYSLCTP-GCKTGALMGCTMKTASCGCHV

64.7%

NZ_FBTD01000003.1:

Lanthipeptides: PB1E_RS03075_lanthipeptide

VTSKSLCTPPGCKTGILMTCAIKTATCGCHF

+TS SLCTP GCKTG LM C +KTA+CGCH

ITSVSLCTP-GCKTGALMGCNMKTASCGCHV

62.9%

NZ_CP013655.1and 2 others:

NZ_CP013655.1 Lanthipeptides: ATZ35_RS03635_lanthipeptideNZ_CP013655.1 Lanthipeptides: ATZ35_RS03630_lanthipeptide

Lanthipeptides: ATZ35_RS03640_lanthipeptide

VTSKSLCTPPGCKTGILMTCAIKTATCGCHFG

+TSKSLCTP GC TG+LM CA+KT TC C G

ITSKSLCTP-GCVTGVLMGCALKTITCNCSVG

62.9%

NZ_FNDP01000013.1and 1 other:

NZ_FNDP01000013.1 Lanthipeptides: BLP54_RS11225_lanthipeptide

Lanthipeptides: BLP54_RS11220_lanthipeptide

VTSKSLCTPPGCKTGILMTCAIKTATCGC--HFG

+TSKSLCTP GC TG+LM CA +ATC C H G

ITSKSLCTP-GCVTGLLMGCAGSSATCNCSVHVG

MIBiG 4.0 matches for M5P35_RS04030

61.8%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

VTSKSLCTPPGCKTGILMTCAIKTATCGC

+TS SLCTP GCKTG LM C +KTATC C

ITSISLCTP-GCKTGALMGCNMKTATCHC

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 26.38 out of 27.78

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.81 out of 20.97

Confidence: medium

Additional weak hits (1):

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 19.31 out of 27.18

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

M5P35_RS04030 - Class I

Cleavage pHMM score: 27.40

RODEO score: 27

Monoisotopic mass: 3084.5 Da

Molecular weight: 3086.7 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 3104.7 Da N = 1; 3122.8 Da N = 2; 3140.8 Da N = 3

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 144 - 226; Score: 25.6

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria