GCF_003468585.1 - 3 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_QSIO01000001.1 (Streptococcus parasanguinis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	T3PKS	892,653	933,825

NZ_QSIO01000002.1 (Streptococcus parasanguinis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 3.1	terpene-precursor	310,654	331,529

NZ_QSIO01000003.1 (Streptococcus parasanguinis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 4.1	lanthipeptide-class-ii	34,588	57,560	Medium	carnolysin A1/carnolysin A2	ribosomal:RiPP:Lanthipeptide

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	T3PKS	892,653	933,825
Region 3.1	terpene-precursor	310,654	331,529
Region 4.1	lanthipeptide-class-ii	34,588	57,560	Medium	carnolysin A1/carnolysin A2	ribosomal:RiPP:Lanthipeptide

No secondary metabolite regions were found in these records:: NZ_QSIO01000010.1 (Streptococcus parasanguinis); NZ_QSIO01000004.1 (Streptococcus parasanguinis); NZ_QSIO01000005.1 (Streptococcus parasanguinis); NZ_QSIO01000006.1 (Streptococcus parasanguinis); NZ_QSIO01000007.1 (Streptococcus parasanguinis); NZ_QSIO01000008.1 (Streptococcus parasanguinis); NZ_QSIO01000009.1 (Streptococcus parasanguinis)

Compact view

NZ_QSIO01000001.1 - Region 1 - T3PKS

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Location: 892,653 - 933,825 nt. (total: 41,173 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: reductase

DW820_RS04540: hydroxymethylglutaryl-CoA reductase

smcogs: SMCOG1187: hydroxymethylglutaryl-CoA reductase

Hydrolases: hydrolase

DW820_RS04585: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Ligases: synthase

DW820_RS04535: hydroxymethylglutaryl-CoA synthase

smcogs: SMCOG1043: hydroxymethylglutaryl-CoA synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002561.3	0.45	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0000558.5	0.42	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0000286.3	0.41	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0000205.3	0.40	PKS	bryostatin	Candidatus Endobugula sertula
BGC0002703.2	0.39	ribosomal	archalan α	Halorussus salinus
BGC0000934.4	0.39	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714
BGC0001080.5	0.39	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0001863.4	0.39	ribosomal, terpene	bacillicn CER074	Bacillus mycoides
BGC0000255.5	0.36	PKS	pederin	Uncultured bacterium
BGC0001606.3	0.35	PKS	gibepyrone-A	Fusarium fujikuroi IMI 58289

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002561.3	0.45	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0000558.5	0.42	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0000286.3	0.41	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0000205.3	0.40	PKS	bryostatin	Candidatus Endobugula sertula
BGC0002703.2	0.39	ribosomal	archalan α	Halorussus salinus
BGC0000934.4	0.39	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714
BGC0001080.5	0.39	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0001863.4	0.39	ribosomal, terpene	bacillicn CER074	Bacillus mycoides
BGC0000255.5	0.36	PKS	pederin	Uncultured bacterium
BGC0001606.3	0.35	PKS	gibepyrone-A	Fusarium fujikuroi IMI 58289

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.31 out of 26.06

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.77 out of 27.78

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.06 out of 19.26

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.04 out of 18.57

Confidence: strong

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.45 out of 26.06

Confidence: medium

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 23.04 out of 29.25

Confidence: medium

Additional weak hits (12):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.99 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.48 out of 26.06

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.40 out of 20.01

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 26.05 out of 30.97

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 21.67 out of 30.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 23.14 out of 31.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.84 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.45 out of 26.06

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.32 out of 30.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.99 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.48 out of 26.06

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
MogR	Repressor of flagellar motility genes
SypG	Regulator of biofilm formation and host colonization
ToxT	Regulator of MSHA biosynthesis

NZ_QSIO01000002.1 - Region 1 - terpene-precursor

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Location: 310,654 - 331,529 nt. (total: 20,876 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

DW820_RS06665: hydrolase

smcogs: SMCOG1179: hydrolase

Isomerases: mutase

DW820_RS06575: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: synthetase

DW820_RS06615: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Translocases: transporter

DW820_RS06650: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0001456.3	0.38	terpene	isorenieratene	Streptomyces argillaceus
BGC0001605.3	0.38	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0000800.5	0.37	saccharide	xanthan	Xanthomonas campestris
BGC0002285.2	0.35	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001594.3	0.35	other (other)	fischerindole L	Fischerella muscicola UTEX 1829
BGC0000668.5	0.34	other (other), terpene	12-epi-hapalindole C isonitrile, 12-epi-hapalindole E, 12-epi-fischerindole U isonitrile, fischerindole L, 12-epi-fischerindole I isonitrile, welwitindolinone A isonitrile, welwitindolinone B isothiocyanate, welwitindolinone C isothiocyanate, N-methylwelwitindolinone C isothiocyanate, N-methylwelwitinsolinone C isonitrile, 3-epi-welwitindolinone B isothiocyanate, 3-(Z-2'-isocyanoethenyl)-indole	Hapalosiphon welwitschii UTEX B 1830
BGC0000648.3	0.34	terpene	carotenoid	Myxococcus xanthus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0001456.3	0.38	terpene	isorenieratene	Streptomyces argillaceus
BGC0001605.3	0.38	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0000800.5	0.37	saccharide	xanthan	Xanthomonas campestris
BGC0002285.2	0.35	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001594.3	0.35	other (other)	fischerindole L	Fischerella muscicola UTEX 1829
BGC0000668.5	0.34	other (other), terpene	12-epi-hapalindole C isonitrile, 12-epi-hapalindole E, 12-epi-fischerindole U isonitrile, fischerindole L, 12-epi-fischerindole I isonitrile, welwitindolinone A isonitrile, welwitindolinone B isothiocyanate, welwitindolinone C isothiocyanate, N-methylwelwitindolinone C isothiocyanate, N-methylwelwitinsolinone C isonitrile, 3-epi-welwitindolinone B isothiocyanate, 3-(Z-2'-isocyanoethenyl)-indole	Hapalosiphon welwitschii UTEX B 1830
BGC0000648.3	0.34	terpene	carotenoid	Myxococcus xanthus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

DW820_RS06615: PT_FPPS_like

Domain 1 (27 - 271): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: DMAPP, IPP → FPP

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.57 out of 18.57

Confidence: strong

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.59 out of 23.05

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 24.72 out of 30.25

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.60 out of 28.19

Confidence: medium

Additional weak hits (5):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.95 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.49 out of 26.06

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 22.66 out of 26.12

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 18.92 out of 30.12

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
ColR	Phenol-responsive regulator
ToxT	Regulator of MSHA biosynthesis
ZuR variant 1	Zinc-responsive repressor

NZ_QSIO01000003.1 - Region 1 - lanthipeptide-class-ii

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Location: 34,588 - 57,560 nt. (total: 22,973 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: protease

DW820_RS07270: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: cyclase

DW820_RS07300: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000500.5	0.62	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0000504.5	0.62	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0000517.4	0.49	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0000622.5	0.49	ribosomal	megacin	Bacillus megaterium
BGC0000623.3	0.48	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0000558.5	0.48	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0001555.4	0.47	ribosomal	colicin V	Escherichia coli chi7122
BGC0002674.2	0.46	ribosomal	enterocin F4-9	Enterococcus faecalis
BGC0000527.5	0.46	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000500.5	0.62	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0000504.5	0.62	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0000517.4	0.49	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0000622.5	0.49	ribosomal	megacin	Bacillus megaterium
BGC0000623.3	0.48	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0000558.5	0.48	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0001555.4	0.47	ribosomal	colicin V	Escherichia coli chi7122
BGC0002674.2	0.46	ribosomal	enterocin F4-9	Enterococcus faecalis
BGC0000527.5	0.46	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for DW820_RS07300

DW820_RS07290

Leader:

MINEIDVNKYAGDSFEEMSIAEMTLVQGS

Core:

GDMSAEFTPTTPACFASIIYTARASSASCANAISAISSAISGAIISAVKC

Core with dehydrations:

GDMDhaAEFDhbPDhbDhbPACysFADhaIIYDhbARADhaDhaADhaCysANAIDhaAIDhaDhaAIDhaGAIIDhaAVKCys

antiSMASH-DB 4.0 matches for DW820_RS07290

100.0%

NZ_QSIO01000003.1:

Lanthipeptides: DW820_RS07290_lanthipeptide

GDMSAEFTPTTPACFASIIYTARASSASCANAISAISSAISGAIISAVKC

86.0%

NZ_JAQCQD010000003.1:

Lanthipeptides: PGZ38_RS05485_lanthipeptide

GDMSAEFTPTTPACFASIIYTARASSASCANAISAISSAISGAIISAVKC

GDMSAE TPTTPACFASIIYTARASS +CA+A+S ISSAISGA+ISAVKC

GDMSAELTPTTPACFASIIYTARASSPNCAHALSVISSAISGAVISAVKC

82.0%

NZ_JAFHCX010000012.1and 1 other:

NZ_LR738722.1 Lanthipeptides: STREPSUIS_RS04080_lanthipeptide

Lanthipeptides: JXW96_RS06960_lanthipeptide

GDMSAEFTPTTPACFASIIYTARASSASCANAISAISSAISGAIISAVKC

GDMSAE TPTTPAC+ASIIYTA+ASS +CA A +AIS AISGAIISAVKC

GDMSAELTPTTPACYASIIYTAKASSPNCAYAATAISGAISGAIISAVKC

with 45 more sequence matches

Only the first 10 are displayed here.

52.0%

NZ_JADNGS010000002.1:

Lanthipeptides: I2D92_RS06835_lanthipeptide

GDMSAEFTPTTPACFASIIYTARASSASCANAISAISSAISGAIISAVKC

GD+S EFT T+PAC + +RASS C A AI+S +S A++SAVKC

GDVSGEFT-TSPACVYVSVAASRASSQKCGQASGAIASFVSTAVLSAVKC

52.0%

NZ_QICS01000003.1:

Lanthipeptides: C8E03_RS08695_lanthipeptide

GDMSAEFTPTTPACFASIIYTARASSASCANAISAISSAISGAIISAVKC

GD+ AEFT T+PAC A + +ASSA CA A + ++S +SGA+ SA+KC

GDVGAEFT-TSPACVALSVAATQASSARCAIASAGVASLVSGAVHSALKC

48.0%

NZ_JQBU01000033.1and 1 other:

NZ_JQBV01000009.1 Lanthipeptides: IV76_RS12140_lanthipeptide

Lanthipeptides: IV75_RS14640_lanthipeptide

GDMSAEFTPTTPACFASIIYTARASSASCANAISAISSAISGAIISAVKC

GD++ EFT T+PAC S++ ++ASSA CA SA+S AI+SA++C

GDINGEFT-TSPACVYSVMVVSKASSAKCAAGASAVSG----AILSAIRC

46.0%

NZ_CP102973.1and 1 other:

NZ_FQQG01000014.1 Lanthipeptides: BT790_RS14440_lanthipeptide

Lanthipeptides: NW963_RS14080_lanthipeptide

GDMSAEFTPTTPACFASIIYTARASSASCANAISAISSAISGAIISAVKC

GDM+ EFT T+PAC S ++ +RASS +C + +A GA++SA+KC

GDMNGEFT-TSPACVYSAVFASRASSKACIGGL----TAAGGAVLSALKC

46.0%

NZ_SDJV01000024.1:

Lanthipeptides: ES032_RS12705_lanthipeptide

GDMSAEFTPTTPACFASIIYTARASSASCANAISAISSAISGAIISAVKC

GD+ E TTPAC S+ TA SS CA A GA+IS VKC

GDVQVE---TTPACLYSVYVTANVSSVWCAG-------ATVGAVISIVKC

44.0%

NZ_JAQDYA010000001.1:

Lanthipeptides: PGT72_RS00025_lanthipeptide

GDMSAEFTPTTPACFASIIYTARASSASCANAISAISSAISGAIISAVKC

GD++ E TTP C A++ ASSA CA A +SA+SG ++S KC

GDITPE---TTPVCAATV-----ASSAYCAGA----ASAVSGIVLSVFKC

42.0%

NZ_CP086395.1and 1 other:

NZ_JAKTAW010000012.1 Lanthipeptides: MKK30_RS11180_lanthipeptide

Lanthipeptides: LMK00_RS10665_lanthipeptide

GDMSAEFTPTTPACFASIIYTARASSASCANAISAISSAISGAIISAVKC

GD+ A T+PAC SI T + SS CA A GA+IS V+C

GDVQAR---TSPACLYSIAVTIKTSSVWCAG-------ASVGAVISIVRC

40.0%

NZ_JAFLVY010000007.1:

Lanthipeptides: JZO73_RS03905_lanthipeptide

GDMSAEFTPTTPACFASIIYTARASSASCANAISAISSAISGAIISAVKC

GD++ EFT T+PAC S++ T + SS CA A GA++S +C

GDVNGEFT-TSPACLYSVMVTLKTSSVPCAG-------ASVGAVVSIARC

39.2%

NZ_CP091282.1:

Lanthipeptides: L2Z53_RS12185_lanthipeptide

GDMSAEFTPTTPACFASIIYTARA--SSASCANAISAISSAISGAIISAVK

GD++AE TP+ S Y A A SSA CA + + +GAI+S+ K

GDVNAETTPSL--IMTSPYYQAAATVSSAGCATGVGGLIGVGAGAILSSTK

38.0%

NZ_CP094446.1:

Lanthipeptides: L3476_RS23825_lanthipeptide

DMSAEFTPTTPACFASIIYTARASSASCANAISAISSAISGAIISAVKC

D+ TP A+I+ SS C ISAI SAISG I C

DVEPRGTP------ATIVSAVTRSSKPC---ISAIGSAISGIISHNKDC

MIBiG 4.0 matches for DW820_RS07290

12.0%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

TPACFASIIYTARASSASCANAI

TP C + +A+C +I

TPGCKTGALMGCNMKTATCHCSI

8.0%

BGC0000582.4 (ADR72962.1):

Linaridin: cypemycin

TPTTPA

TP TP

TPATPT

DW820_RS07295

Leader:

MLDVQDSIVTGAAFEDMSIAEMTRVQGS

Core:

GDISPETTPACVLIISNVISGAAVSFKFSVDVVKTLKGKC

Core with dehydrations:

GDIDhaPEDhbDhbPACysVLIIDhaNVIDhaGAAVDhaFKFDhaVDVVKDhbLKGKCys

antiSMASH-DB 4.0 matches for DW820_RS07295

100.0%

NZ_QSIO01000003.1:

Lanthipeptides: DW820_RS07295_lanthipeptide

GDISPETTPACVLIISNVISGAAVSFKFSVDVVKTLKGKC

87.5%

NZ_JAQCQD010000003.1:

Lanthipeptides: PGZ38_RS05480_lanthipeptide

GDISPETTPACVLIISNVISGAAVSFKFSVDVVKTLKGKC

GDISPETTPAC LIIS +ISGAAVSF FSV VVKTLKGKC

GDISPETTPACALIISKIISGAAVSFGFSVSVVKTLKGKC

82.5%

NZ_JAFHCX010000012.1and 1 other:

NZ_LR738722.1 Lanthipeptides: STREPSUIS_RS04075_lanthipeptide

Lanthipeptides: JXW96_RS06965_lanthipeptide

GDISPETTPACVLIISNVISGAAVSFKFSVDVVKTLKGKC

GDISPETTPAC LIIS ISGAAVSF FSV VVKT+KG C

GDISPETTPACALIISKAISGAAVSFAFSVGVVKTVKGNC

with 110 more sequence matches

Only the first 10 are displayed here.

45.2%

NZ_CP010976.1:

Lanthipeptides: UB51_RS20985_lanthipeptide

GDISPETTPA--CVLIISNVISGAAVSFKFSVDVVKTLKGKC

GD++PETTPA ++I+S + AA S S+ V+T+KG C

GDVNPETTPATPTIVIVSEGLLTAAGSAALSILSVRTIKGHC

45.0%

NZ_JADMPO010000001.1:

Lanthipeptides: I2I27_RS01390_lanthipeptide

GDISPE-TTPACVLIISNVISGAAVSFKFSVDVVKTLKGKC

GD+ E TTP C + AV+ S+ V KTLKGKC

GDVEGEVTTPICAV---------AVTASLSIQVAKTLKGKC

42.5%

NZ_CP102973.1and 1 other:

NZ_FQQG01000014.1 Lanthipeptides: BT790_RS14420_lanthipeptide

Lanthipeptides: NW963_RS14100_lanthipeptide

GDISPETTPACVLIISNVISGAAVSFKFSVDVVKTLKGKC

GD++PE+T C A SF S+ +VKTLK KC

GDVTPESTTVC---------AAFASFAGSIQLVKTLKSKC

42.5%

NZ_JADNGS010000002.1:

Lanthipeptides: I2D92_RS06840_lanthipeptide

GDISPETTPACVLIISNVISGAAVSFKFSVDVVKTLKGKC

GDI P + + + S V+ AA + FS+ V KT+KGKC

GDIEPNS----LTVASAVLMSAAATGSFSIAVTKTVKGKC

42.5%

NZ_QICS01000003.1:

Lanthipeptides: C8E03_RS08700_lanthipeptide

GDISPETTPACVLIISNVISGAAVSFKFSVDVVKTLKGKC

GD++ ETT + ++ + AA S+ V VVKT+KGKC

GDVNAETTIELFISVTLAATAAAGSYASGVQVVKTIKGKC

42.5%

NZ_SDJV01000024.1:

Lanthipeptides: ES032_RS12700_lanthipeptide

GDISPETTPACVLIISNVISGAAVSFKFSVDVVKTLKGKC

GD+ ETTPAC S ++ S VVKT+KG C

GDVQVETTPACAFTASILV---------SYGVVKTIKGHC

40.0%

NZ_CP091282.1:

Lanthipeptides: L2Z53_RS12190_lanthipeptide

GDISPETTPACVLIISNVISGAAVSFKFSVDVVKTLKGKC

GD++ ETTP IS + ++ S+ V KT+KGKC

GDVNVETTPTAT--ISAFLLSVGGGYELSIHVAKTVKGKC

40.0%

NZ_FPCC01000026.1:

Lanthipeptides: BM377_RS12935_lanthipeptide

GDISPETTPACVLIISNVISGAAVSFKFSVDVVKTLKGKC

GD+ ETTPA + + + V+ G ++ F+ +V V KT G C

GDVDAETTPA-ITVSAAVVIGGSLGFEATVKVTKTCNGNC

37.5%

NZ_AP026721.1and 35 others:

NZ_CP015883.1 Lanthipeptides: A6B47_RS00875_lanthipeptideNZ_CP028286.1 Lanthipeptides: CEQ02_RS16115_lanthipeptideNZ_CP028721.1 Lanthipeptides: C7I32_RS15475_lanthipeptideNZ_CP028835.1 Lanthipeptides: C9432_RS02740_lanthipeptideNZ_CP028837.1 Lanthipeptides: C9432_RS15935_lanthipeptideNZ_CP033787.1 Lanthipeptides: EGX75_RS02740_lanthipeptideNZ_CP039296.1 Lanthipeptides: E7T02_RS02755_lanthipeptideNZ_CP045045.1 Lanthipeptides: F9293_RS12755_lanthipeptideNZ_CP075604.2 Lanthipeptides: KKP64_RS02155_lanthipeptideNZ_CP085289.1 Lanthipeptides: LIT95_RS07630_lanthipeptideNZ_CP085291.1 Lanthipeptides: LIT96_RS04335_lanthipeptideNZ_CP085297.1 Lanthipeptides: LIT97_RS14520_lanthipeptideNZ_CP086726.1 Lanthipeptides: LM507_RS03415_lanthipeptideNZ_CP091901.1 Lanthipeptides: L5I17_RS09010_lanthipeptideNZ_CP091905.1 Lanthipeptides: L5I20_RS14940_lanthipeptideNZ_CP091907.1 Lanthipeptides: L5I26_RS13265_lanthipeptideNZ_CP093957.1 Lanthipeptides: MPM64_RS01385_lanthipeptideNZ_CP116962.1 Lanthipeptides: PO880_RS13030_lanthipeptideNZ_JAASJP010000011.1 Lanthipeptides: HCH27_RS13275_lanthipeptideNZ_JAHZNN010000041.1 Lanthipeptides: K0X81_RS16410_lanthipeptideNZ_JAKTAU010000028.1 Lanthipeptides: MKK46_RS14535_lanthipeptideNZ_JALSCL010000035.1 Lanthipeptides: NEI30_RS13375_lanthipeptideNZ_JAQDOF010000024.1 Lanthipeptides: PGP79_RS14595_lanthipeptideNZ_JAQKZU010000014.1 Lanthipeptides: PMV60_RS12615_lanthipeptideNZ_KB932317.1 Lanthipeptides: Q95_RS09840_lanthipeptideNZ_KB932336.1 Lanthipeptides: Q9C_RS13350_lanthipeptideNZ_KB944697.1 Lanthipeptides: WO3_RS18030_lanthipeptideNZ_KB944708.1 Lanthipeptides: WO5_RS05650_lanthipeptideNZ_KB944753.1 Lanthipeptides: WO9_RS06460_lanthipeptideNZ_KB944762.1 Lanthipeptides: WOA_RS17470_lanthipeptideNZ_KB944819.1 Lanthipeptides: WOK_RS14355_lanthipeptideNZ_KB946767.1 Lanthipeptides: UK1_RS12990_lanthipeptideNZ_KB947483.1 Lanthipeptides: WUI_RS10440_lanthipeptideNZ_KZ845881.1 Lanthipeptides: EB41_RS04380_lanthipeptideNZ_NGMY01000001.1 Lanthipeptides: A5862_RS02370_lanthipeptide

Lanthipeptides: PO855_RS02215_lanthipeptide

GDISPETTPACVLIISNVISGAAVSFKF

GD+ ETTPAC I V GA S KF

GDVQAETTPACFTIGLGV--GALFSAKF

37.5%

NZ_CP086395.1and 1 other:

NZ_JAKTAW010000012.1 Lanthipeptides: MKK30_RS11185_lanthipeptide

Lanthipeptides: LMK00_RS10670_lanthipeptide

GDISPETTPACVLIISNVISGAAVSFKFSVDVVKTLKGKC

GD+ +TP C +G VS + +VKTLKG+C

GDVEVRSTPICTFT-----AGVTVS----IVIVKTLKGRC

allorf_035930_036094

Leader:

MLAIGIEKICFEGARRRMMLVTYS

Core:

FCLVSFFDRIGTIFDRLVKSPCRRWGSLLS

Core with dehydrations:

FCysLVDhaFFDRIGDhbIFDRLVKDhaPCysRRWGDhaLLDha

antiSMASH-DB 4.0 matches for allorf_035930_036094

100.0%

NZ_QSIO01000003.1:

Lanthipeptides: allorf_035930_036094_lanthipeptide

FCLVSFFDRIGTIFDRLVKSPCRRWGSLLS

16.7%

NZ_LMSL01000013.1:

Lanthipeptides: allorf_00156_00260_lanthipeptide

DRIGTIFDRLVKSPCR

+RIG + + +PC+

NRIGGLDETTAGAPCK

14.0%

NC_017941.2:

Lanthipeptides: allorf_0828594_0828770_lanthipeptide

VKSPCRRWG

VK+ CRR+G

VKTGCRRYG

with 2 more sequence matches

12.0%

NZ_JALPRT010000019.1and 2 others:

NZ_KB892552.1 Lanthipeptides: allorf_24609_24818_lanthipeptideNZ_KB896162.1 Lanthipeptides: allorf_034118_034327_lanthipeptide

Lanthipeptides: allorf_071360_071569_lanthipeptide

IGTIFDRLVKSP

+ T+ DRL +SP

VHTLVDRLTQSP

11.5%

NZ_QTVL01000002.1and 2 others:

NZ_QTZB01000015.1 Lanthipeptides: DXC15_RS14275_lanthipeptideNZ_QUKU01000003.1 Lanthipeptides: DXC27_RS03000_lanthipeptide

Lanthipeptides: DWZ44_RS04090_lanthipeptide

DRIGTIFDRLVKSPCRR

++I + F +KS CRR

NQILSYFQPFIKSECRR

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.08 out of 26.85

Confidence: medium

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.80 out of 26.33

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.52 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.95 out of 26.06

Confidence: medium

Additional weak hits (7):

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 15.62 out of 18.46

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.35 out of 26.33

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 25.35 out of 31.73

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 20.96 out of 30.22

Confidence: weak

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.04 out of 20.47

Confidence: weak

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 16.10 out of 20.47

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.73 out of 32.74

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

DW820_RS07290 - Class II

Cleavage pHMM score: -3.70

RODEO score: 0

Monoisotopic mass: 4631.3 Da

Molecular weight: 4634.2 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 4652.3 Da N = 1; 4670.3 Da N = 2; 4688.3 Da N = 3; 4706.3 Da N = 4; 4724.3 Da N = 5; 4742.4 Da N = 6; 4760.4 Da N = 7; 4778.4 Da N = 8; 4796.4 Da N = 9; 4814.4 Da N = 10; 4832.5 Da N = 11

DW820_RS07295 - Class II

Cleavage pHMM score: -2.00

RODEO score: 0

Monoisotopic mass: 3949.2 Da

Molecular weight: 3951.6 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3969.6 Da N = 1; 3987.7 Da N = 2; 4005.7 Da N = 3; 4023.7 Da N = 4; 4041.7 Da N = 5; 4059.7 Da N = 6

allorf_035930_036094 - Class II

Cleavage pHMM score: -7.30

RODEO score: 0

Monoisotopic mass: 3427.8 Da

Molecular weight: 3430.0 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3448.1 Da N = 1; 3466.1 Da N = 2; 3484.1 Da N = 3

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
MogR	Repressor of flagellar motility genes
RutR	Regulator of the pyrimidine and purine metabolism