GCF_003467665.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_QSHK01000001.1 (Dorea formicigenerans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	terpene-precursor	106,343	127,245
Region 1.2	ranthipeptide	592,796	614,458

NZ_QSHK01000018.1 (Dorea formicigenerans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 10.1	cyclic-lactone-autoinducer	8,891	29,481

NZ_QSHK01000002.1 (Dorea formicigenerans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 12.1	cyclic-lactone-autoinducer,lanthipeptide-class-i	198,378	230,440	Medium	nisin O	ribosomal:RiPP:Lanthipeptide

NZ_QSHK01000008.1 (Dorea formicigenerans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 37.1	t3nrps-iterative	101,805	139,798

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	terpene-precursor	106,343	127,245
Region 1.2	ranthipeptide	592,796	614,458
Region 10.1	cyclic-lactone-autoinducer	8,891	29,481
Region 12.1	cyclic-lactone-autoinducer,lanthipeptide-class-i	198,378	230,440	Medium	nisin O	ribosomal:RiPP:Lanthipeptide
Region 37.1	t3nrps-iterative	101,805	139,798

No secondary metabolite regions were found in these records:: NZ_QSHK01000010.1 (Dorea formicigenerans); NZ_QSHK01000011.1 (Dorea formicigenerans); NZ_QSHK01000012.1 (Dorea formicigenerans); NZ_QSHK01000013.1 (Dorea formicigenerans); NZ_QSHK01000014.1 (Dorea formicigenerans); NZ_QSHK01000015.1 (Dorea formicigenerans); NZ_QSHK01000016.1 (Dorea formicigenerans); NZ_QSHK01000017.1 (Dorea formicigenerans); NZ_QSHK01000019.1 (Dorea formicigenerans); NZ_QSHK01000020.1 (Dorea formicigenerans); NZ_QSHK01000021.1 (Dorea formicigenerans); NZ_QSHK01000022.1 (Dorea formicigenerans); NZ_QSHK01000023.1 (Dorea formicigenerans); NZ_QSHK01000024.1 (Dorea formicigenerans); NZ_QSHK01000025.1 (Dorea formicigenerans); NZ_QSHK01000026.1 (Dorea formicigenerans); NZ_QSHK01000027.1 (Dorea formicigenerans); NZ_QSHK01000028.1 (Dorea formicigenerans); NZ_QSHK01000029.1 (Dorea formicigenerans); NZ_QSHK01000003.1 (Dorea formicigenerans); NZ_QSHK01000030.1 (Dorea formicigenerans); NZ_QSHK01000031.1 (Dorea formicigenerans); NZ_QSHK01000032.1 (Dorea formicigenerans); NZ_QSHK01000033.1 (Dorea formicigenerans); NZ_QSHK01000034.1 (Dorea formicigenerans); NZ_QSHK01000035.1 (Dorea formicigenerans); NZ_QSHK01000036.1 (Dorea formicigenerans); NZ_QSHK01000037.1 (Dorea formicigenerans); NZ_QSHK01000038.1 (Dorea formicigenerans); NZ_QSHK01000004.1 (Dorea formicigenerans); NZ_QSHK01000005.1 (Dorea formicigenerans); NZ_QSHK01000006.1 (Dorea formicigenerans); NZ_QSHK01000007.1 (Dorea formicigenerans); NZ_QSHK01000009.1 (Dorea formicigenerans)

Compact view

NZ_QSHK01000001.1 - Region 1 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 106,343 - 127,245 nt. (total: 20,903 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: glycosyltransferase

DW860_RS00625: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

Hydrolases: back translocase

DW860_RS00560: GTP-binding protein LepA

smcogs: SMCOG1190: GTP-binding protein LepA

Ligases: synthase synthetase

DW860_RS00585: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

DW860_RS00590: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0001605.3	0.38	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0000665.5	0.37	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0001604.3	0.37	terpene	gibberellin	Fusarium fujikuroi
BGC0001227.3	0.36	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000663.5	0.36	terpene	hopene	Streptomyces coelicolor A3(2)

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0001605.3	0.38	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0000665.5	0.37	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0001604.3	0.37	terpene	gibberellin	Fusarium fujikuroi
BGC0001227.3	0.36	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000663.5	0.36	terpene	hopene	Streptomyces coelicolor A3(2)

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

DW860_RS00585: PT_FPPS_like

Domain 1 (36 - 299): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: IPP, DMAPP → FPP

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.57 out of 18.57

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.66 out of 23.79

Confidence: medium

Additional weak hits (1):

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.72 out of 30.98

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
ToxT	Regulator of MSHA biosynthesis

NZ_QSHK01000001.1 - Region 2 - ranthipeptide

Download region GenBank file

Download region SVG

Location: 592,796 - 614,458 nt. (total: 21,663 nt) Show pHMM detection rules used

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: transferase

DW860_RS02940: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

DW860_RS02945: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

Hydrolases: hydrolase

DW860_RS02870: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002306.2	0.47	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0001387.5	0.37	other (other)	nucleocidin	Streptomyces calvus
BGC0001995.3	0.37	terpene	heptelidic acid	Aspergillus oryzae RIB40
BGC0000678.5	0.36	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0002436.2	0.34	other (other)	coformycin	Streptomyces citricolor
BGC0001002.5	0.34	NRPS, PKS	jawsamycin	Streptomyces roseoverticillatus
BGC0001591.4	0.33	other (other)	fatty acid enol ester, N-tetradecanoyl tyrosine	uncultured bacterium CSLC2
BGC0001607.5	0.33	other (other)	gilvusmycin	Streptomyces sp. NRRL B-1347
BGC0000613.5	0.32	ribosomal	thiomuracin A	Nonomuraea sp. Bp3714-39
BGC0000653.5	0.31	terpene	pentalenolactone	Streptomyces arenae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002306.2	0.47	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0001387.5	0.37	other (other)	nucleocidin	Streptomyces calvus
BGC0001995.3	0.37	terpene	heptelidic acid	Aspergillus oryzae RIB40
BGC0000678.5	0.36	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0002436.2	0.34	other (other)	coformycin	Streptomyces citricolor
BGC0001002.5	0.34	NRPS, PKS	jawsamycin	Streptomyces roseoverticillatus
BGC0001591.4	0.33	other (other)	fatty acid enol ester, N-tetradecanoyl tyrosine	uncultured bacterium CSLC2
BGC0001607.5	0.33	other (other)	gilvusmycin	Streptomyces sp. NRRL B-1347
BGC0000613.5	0.32	ribosomal	thiomuracin A	Nonomuraea sp. Bp3714-39
BGC0000653.5	0.31	terpene	pentalenolactone	Streptomyces arenae

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.07 out of 18.46

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.91 out of 26.06

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.88 out of 20.47

Confidence: medium

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.55 out of 21.55

Confidence: strong

Additional weak hits (1):

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.79 out of 22.40

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: Ranthipeptide_rSAM_RRE; Location: 0 - 111; Score: 102.2

TFBS definitions

Regulator	Description
ArcA	Repressor of carbon oxidation
MogR	Repressor of flagellar motility genes
RpoN	Regulator of nitrogen assimilation and virulence
VqsM	Regulator of quorum-sensing signalling systems

NZ_QSHK01000018.1 - Region 1 - cyclic-lactone-autoinducer

Download region GenBank file

Download region SVG

Location: 8,891 - 29,481 nt. (total: 20,591 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	cyclic-lactone-autoinducer	Similarity score	Type	Compound(s)	Organism
BGC0000158.5		0.19	PKS	tautomycetin	Streptomyces griseochromogenes

Reference	Aggregated	Similarity score	Type	Compound(s)	Organism
BGC0000158.5		0.19	PKS	tautomycetin	Streptomyces griseochromogenes

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.83 out of 26.33

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.23 out of 19.52

Confidence: medium

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 24.74 out of 28.14

Confidence: medium

Additional weak hits (2):

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.99 out of 27.55

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 20.68 out of 26.12

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
PerR	Peroxide regulator

NZ_QSHK01000002.1 - Region 1 - cyclic-lactone-autoinducer,lanthipeptide-class-i

Download region GenBank file

Download region SVG

Location: 198,378 - 230,440 nt. (total: 32,063 nt) Show pHMM detection rules used

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)
lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase dehydratase

DW860_RS04695: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

DW860_RS04705: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001701.4	0.61	ribosomal	nisin O	Blautia obeum
BGC0000538.3	0.47	ribosomal	nisin Z	Lactococcus lactis
BGC0001289.5	0.43	ribosomal	penisin	Paenibacillus ehimensis
BGC0002328.3	0.37	ribosomal	Rc-AIP1	Ruminiclostridium cellulolyticum H10
BGC0000515.4	0.34	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000542.3	0.34	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000541.5	0.34	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000506.4	0.34	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000559.4	0.31	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0002668.2	0.31	ribosomal	cesin A	Clostridium estertheticum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001701.4	0.80	ribosomal	nisin O	Blautia obeum
BGC0000538.3	0.65	ribosomal	nisin Z	Lactococcus lactis
BGC0001289.5	0.59	ribosomal	penisin	Paenibacillus ehimensis
BGC0000515.4	0.57	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000542.3	0.56	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000506.4	0.56	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000541.5	0.56	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0002668.2	0.55	ribosomal	cesin A	Clostridium estertheticum
BGC0000559.4	0.54	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0000535.4	0.53	ribosomal	nisin A	Lactococcus lactis subsp. lactis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for DW860_RS04705

DW860_RS04730

Leader:

MAKFDDFDLDVTKTAAQGGIEPK

DW860_RS04735

Leader:

MAKFDDFDLDVTKTAAQGGIEPK

Shared core:

YKSKSVCTPGCPTGILMTCPLKTATCGCHITGK

Core with dehydrations:

YKDhaKDhaVCysDhbPGCysPDhbGILMDhbCysPLKDhbADhbCysGCysHIDhbGK

antiSMASH-DB 4.0 matches for DW860_RS04730

100.0%

NZ_QSHK01000002.1and 1 other:

NZ_QSHK01000002.1 Lanthipeptides: DW860_RS04730_lanthipeptide

Lanthipeptides: DW860_RS04735_lanthipeptide

YKSKSVCTPGCPTGILMTCPLKTATCGCHITGK

97.0%

NZ_JADNJR010000016.1and 2 others:

NZ_JADNJR010000016.1 Lanthipeptides: I2E58_RS10875_lanthipeptideNZ_QSHK01000002.1 Lanthipeptides: DW860_RS04740_lanthipeptide

Lanthipeptides: I2E58_RS10880_lanthipeptide

YKSKSVCTPGCPTGILMTCPLKTATCGCHITGK

YKSKS CTPGCPTGILMTCPLKTATCGCHITGK

YKSKSACTPGCPTGILMTCPLKTATCGCHITGK

78.8%

NZ_CP015405.2and 7 others:

NZ_CP015405.2 Lanthipeptides: A4V09_RS08295_lanthipeptideNZ_CP015405.2 Lanthipeptides: A4V09_RS08290_lanthipeptideNZ_CP015405.2 Lanthipeptides: A4V09_RS08285_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06240_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06235_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06230_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06225_lanthipeptide

Lanthipeptides: A4V09_RS08300_lanthipeptide

SKSVCTPGCPTGILMTCPLKTATCGCHITGK

SKS+CTPGC TGILMTCP++TATCGC ITGK

SKSLCTPGCVTGILMTCPVQTATCGCQITGK

with 80 more sequence matches

Only the first 10 are displayed here.

69.7%

NC_015600.1and 14 others:

NZ_AP020310.1 Lanthipeptides: SAGCMC97051_RS07300_lanthipeptideNZ_ATVP01000001.1 Lanthipeptides: G574_RS11150_lanthipeptideNZ_BCFB01000042.1 Lanthipeptides: GOZ36_RS07980_lanthipeptideNZ_CP068708.1 Lanthipeptides: JM964_RS04040_lanthipeptideNZ_CP116958.1 Lanthipeptides: M0P28_RS07755_lanthipeptideNZ_CP116958.1 Lanthipeptides: M0P28_RS07755_lanthipeptideNZ_FIKS01000005.1 Lanthipeptides: A0525_RS10015_lanthipeptideNZ_GL636070.1 Lanthipeptides: HMPREF9171_RS106200_lanthipeptideNZ_JADPLU010000018.1 Lanthipeptides: I4V82_RS09545_lanthipeptideNZ_JAKVIE010000017.1 Lanthipeptides: MK857_RS08340_lanthipeptideNZ_JAQDQV010000001.1 Lanthipeptides: PGO74_RS00635_lanthipeptideNZ_JAQDQV010000001.1 Lanthipeptides: PGO74_RS00635_lanthipeptideNZ_LR594050.1 Lanthipeptides: FGL20_RS03485_lanthipeptideNZ_SSXN01000004.1 Lanthipeptides: FAJ36_RS04715_lanthipeptide

Lanthipeptides: SGPB_RS10680_lanthipeptide

SKSVCTPGCPTGILMTCPLKTATCGCHI

SKS+CTPGC TGILMTC +KTATCGCH

SKSLCTPGCKTGILMTCAIKTATCGCHF

69.7%

NZ_CP059471.1and 3 others:

NZ_FNZW01000001.1 Lanthipeptides: BMZ61_RS02110_lanthipeptideNZ_FOYC01000001.1 Lanthipeptides: BMZ88_RS03370_lanthipeptideNZ_KE384124.1 Lanthipeptides: G597_RS0106700_lanthipeptide

Lanthipeptides: H1R75_RS06635_lanthipeptide

SKSVCTPGCPTGILMTCPLKTATCGCHI

SKS+CTPGC TG LMTCP+KTATCGCH

SKSLCTPGCKTGALMTCPIKTATCGCHF

69.7%

NZ_JAMDIB010000004.1:

Lanthipeptides: M5P35_RS04030_lanthipeptide

SKSVCTP-GCPTGILMTCPLKTATCGCHI

SKS+CTP GC TGILMTC +KTATCGCH

SKSLCTPPGCKTGILMTCAIKTATCGCHF

66.7%

NZ_CP024974.1and 1 other:

NZ_JAIMDQ010000006.1 Lanthipeptides: K6V44_RS04045_lanthipeptide

Lanthipeptides: CVO91_RS06555_lanthipeptide

SKSVCTPGCPTGILMTCPLKTATCGCHI

S S+CTPGC TGILMTC +KTATCGCH

SISLCTPGCKTGILMTCAIKTATCGCHF

63.6%

NZ_BMMB01000002.1:

Lanthipeptides: IEX62_RS05935_lanthipeptide

YKSKSVCTPGCPTGILMTCPLKTATCGCHIT

+KSKS CTPGC TG+L TC ++TATC CHI+

WKSKSFCTPGCVTGVLQTCFIQTATCNCHIS

63.6%

NZ_CP025079.1and 3 others:

NZ_CP047644.1 Lanthipeptides: GNE05_RS17275_lanthipeptideNZ_CP099859.1 Lanthipeptides: NIZ95_RS16280_lanthipeptideNZ_CP101621.1 Lanthipeptides: NN913_RS16115_lanthipeptide

Lanthipeptides: BVQ_RS17705_lanthipeptide

YKSKSVCTPGCPTGILMTCPLKTATCGCHIT

+KS+SVCTPGC TG+L TC L+T TC CHI+

WKSESVCTPGCVTGVLQTCFLQTITCNCHIS

60.6%

NC_016047.1and 2 others:

NZ_KZ848487.1 Lanthipeptides: DSL71_RS15965_lanthipeptideNZ_MUXL01000003.1 Lanthipeptides: B0W20_RS12275_lanthipeptide

Lanthipeptides: GYO_RS39160_lanthipeptide

YKSKSVCTPGCPTGILMTCPLKTATCGCHIT

+KS+SVCTPGC TG+L TC L+T TC C I+

WKSESVCTPGCVTGLLQTCFLQTITCNCKIS

60.6%

NZ_CP117416.1and 1 other:

NZ_JAQQZQ010000001.1 Lanthipeptides: PO903_RS06885_lanthipeptide

Lanthipeptides: PQ456_RS01230_lanthipeptide

YKSKSVCTPGCPTGILMTCPLKTATCGCHIT

+KS+S CTPGC TG+L TC ++TATC CHI+

WKSQSFCTPGCVTGVLQTCFIQTATCNCHIS

60.0%

NZ_NHVL01000013.1:

Lanthipeptides: CEU70_RS16830_lanthipeptide

SKSVCTPGCPTGILMTCPLKTATCGC--HITGK

SKS+CTPGC TGIL TC +K+ATCGC HI GK

SKSLCTPGCKTGILQTCAIKSATCGCSIHIGGK

58.8%

NZ_JADNFO010000025.1:

Lanthipeptides: I2C89_RS09745_lanthipeptide

SKSVCTPGCPTGILMTCPLKTATCGCHI

S S+CTPGC TG+LM C LKTATC C I

SYSLCTPGCKTGVLMACHLKTATCNCSI

MIBiG 4.0 matches for DW860_RS04730

55.9%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

SKSVCTPGCPTGILMTCPLKTATCGCHI

S S+CTPGC TG LM C +KTATC C I

SISLCTPGCKTGALMGCNMKTATCHCSI

12.1%

BGC0000582.4 (ADR72962.1):

Linaridin: cypemycin

TPGCPT

TP PT

TPATPT

DW860_RS04740

Leader:

MAKFDDFDLDVTKTAARGGIEPK

Core:

YKSKSACTPGCPTGILMTCPLKTATCGCHITGK

Core with dehydrations:

YKDhaKDhaACysDhbPGCysPDhbGILMDhbCysPLKDhbADhbCysGCysHIDhbGK

antiSMASH-DB 4.0 matches for DW860_RS04740

100.0%

NZ_JADNJR010000016.1and 2 others:

NZ_JADNJR010000016.1 Lanthipeptides: I2E58_RS10875_lanthipeptideNZ_QSHK01000002.1 Lanthipeptides: DW860_RS04740_lanthipeptide

Lanthipeptides: I2E58_RS10880_lanthipeptide

YKSKSACTPGCPTGILMTCPLKTATCGCHITGK

97.0%

NZ_QSHK01000002.1and 1 other:

NZ_QSHK01000002.1 Lanthipeptides: DW860_RS04730_lanthipeptide

Lanthipeptides: DW860_RS04735_lanthipeptide

YKSKSACTPGCPTGILMTCPLKTATCGCHITGK

YKSKS CTPGCPTGILMTCPLKTATCGCHITGK

YKSKSVCTPGCPTGILMTCPLKTATCGCHITGK

78.8%

NZ_CP015405.2and 7 others:

Lanthipeptides: A4V09_RS08300_lanthipeptide

SKSACTPGCPTGILMTCPLKTATCGCHITGK

SKS CTPGC TGILMTCP++TATCGC ITGK

SKSLCTPGCVTGILMTCPVQTATCGCQITGK

with 71 more sequence matches

Only the first 10 are displayed here.

69.7%

NC_015600.1and 14 others:

Lanthipeptides: SGPB_RS10680_lanthipeptide

SKSACTPGCPTGILMTCPLKTATCGCHI

SKS CTPGC TGILMTC +KTATCGCH

SKSLCTPGCKTGILMTCAIKTATCGCHF

69.7%

NZ_CP059471.1and 3 others:

NZ_FNZW01000001.1 Lanthipeptides: BMZ61_RS02110_lanthipeptideNZ_FOYC01000001.1 Lanthipeptides: BMZ88_RS03370_lanthipeptideNZ_KE384124.1 Lanthipeptides: G597_RS0106700_lanthipeptide

Lanthipeptides: H1R75_RS06635_lanthipeptide

SKSACTPGCPTGILMTCPLKTATCGCHI

SKS CTPGC TG LMTCP+KTATCGCH

SKSLCTPGCKTGALMTCPIKTATCGCHF

69.7%

NZ_JAMDIB010000004.1:

Lanthipeptides: M5P35_RS04030_lanthipeptide

SKSACTP-GCPTGILMTCPLKTATCGCHI

SKS CTP GC TGILMTC +KTATCGCH

SKSLCTPPGCKTGILMTCAIKTATCGCHF

66.7%

NZ_CP024974.1and 1 other:

NZ_JAIMDQ010000006.1 Lanthipeptides: K6V44_RS04045_lanthipeptide

Lanthipeptides: CVO91_RS06555_lanthipeptide

SKSACTPGCPTGILMTCPLKTATCGCHI

S S CTPGC TGILMTC +KTATCGCH

SISLCTPGCKTGILMTCAIKTATCGCHF

63.6%

NZ_BMMB01000002.1:

Lanthipeptides: IEX62_RS05935_lanthipeptide

YKSKSACTPGCPTGILMTCPLKTATCGCHIT

+KSKS CTPGC TG+L TC ++TATC CHI+

WKSKSFCTPGCVTGVLQTCFIQTATCNCHIS

60.6%

NZ_CP025079.1and 3 others:

NZ_CP047644.1 Lanthipeptides: GNE05_RS17275_lanthipeptideNZ_CP099859.1 Lanthipeptides: NIZ95_RS16280_lanthipeptideNZ_CP101621.1 Lanthipeptides: NN913_RS16115_lanthipeptide

Lanthipeptides: BVQ_RS17705_lanthipeptide

YKSKSACTPGCPTGILMTCPLKTATCGCHIT

+KS+S CTPGC TG+L TC L+T TC CHI+

WKSESVCTPGCVTGVLQTCFLQTITCNCHIS

60.6%

NZ_CP117416.1and 1 other:

NZ_JAQQZQ010000001.1 Lanthipeptides: PO903_RS06885_lanthipeptide

Lanthipeptides: PQ456_RS01230_lanthipeptide

YKSKSACTPGCPTGILMTCPLKTATCGCHIT

+KS+S CTPGC TG+L TC ++TATC CHI+

WKSQSFCTPGCVTGVLQTCFIQTATCNCHIS

60.0%

NZ_NHVL01000013.1:

Lanthipeptides: CEU70_RS16830_lanthipeptide

SKSACTPGCPTGILMTCPLKTATCGC--HITGK

SKS CTPGC TGIL TC +K+ATCGC HI GK

SKSLCTPGCKTGILQTCAIKSATCGCSIHIGGK

58.8%

NZ_JADNFO010000025.1:

Lanthipeptides: I2C89_RS09745_lanthipeptide

SKSACTPGCPTGILMTCPLKTATCGCHI

S S CTPGC TG+LM C LKTATC C I

SYSLCTPGCKTGVLMACHLKTATCNCSI

58.3%

NZ_NHVL01000013.1:

Lanthipeptides: CEU70_RS16825_lanthipeptide

SKSACTPGCPTGILMTCPLKTATCGCHI

SKS CTPGC TGIL TC +K+ATCGC I

SKSLCTPGCKTGILQTCAIKSATCGCSI

MIBiG 4.0 matches for DW860_RS04740

55.9%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

SKSACTPGCPTGILMTCPLKTATCGCHI

S S CTPGC TG LM C +KTATC C I

SISLCTPGCKTGALMGCNMKTATCHCSI

12.1%

BGC0000582.4 (ADR72962.1):

Linaridin: cypemycin

TPGCPT

TP PT

TPATPT

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.28 out of 23.05

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.15 out of 27.78

Confidence: medium

Additional weak hits (1):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.27 out of 25.75

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

DW860_RS04730 - Class I

Cleavage pHMM score: 12.50

RODEO score: 26

Monoisotopic mass: 3254.6 Da

Molecular weight: 3256.9 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 3275.0 Da N = 1; 3293.0 Da N = 2; 3311.0 Da N = 3

DW860_RS04735 - Class I

Cleavage pHMM score: 12.50

RODEO score: 26

Monoisotopic mass: 3254.6 Da

Molecular weight: 3256.9 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 3275.0 Da N = 1; 3293.0 Da N = 2; 3311.0 Da N = 3

DW860_RS04740 - Class I

Cleavage pHMM score: 9.70

RODEO score: 26

Monoisotopic mass: 3226.6 Da

Molecular weight: 3228.9 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 3246.9 Da N = 1; 3264.9 Da N = 2; 3282.9 Da N = 3

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 142 - 228; Score: 45.4

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria

NZ_QSHK01000008.1 - Region 1 - t3nrps-iterative

Download region GenBank file

Download region SVG

Location: 101,805 - 139,798 nt. (total: 37,994 nt) Show pHMM detection rules used

Region on contig edge.

t3nrps-iterative: (AMP-binding and PP-binding and (DUF98 or Orn_Arg_deC_N or Orn_DAP_Arg_deC) and not ATP-grasp)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: decarboxylase

DW860_RS11665: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

Ligases: ligase synthase

DW860_RS11660: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

DW860_RS11705: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.60	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001641.4	0.59	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.59	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.59	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.59	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.59	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002164.2	0.58	NRPS	peramine	Epichloe festucae
BGC0001825.4	0.58	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002518.3	0.45	NRPS	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001844.4	0.44	NRPS	holrhizin A, holrhizin B, holrhizin C, holrhizin D	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.60	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001641.4	0.59	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.59	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.59	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.59	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.59	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002164.2	0.58	NRPS	peramine	Epichloe festucae
BGC0001825.4	0.58	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002518.3	0.45	NRPS	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001844.4	0.44	NRPS	holrhizin A, holrhizin B, holrhizin C, holrhizin D	Paraburkholderia rhizoxinica HKI 454

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.06 out of 27.78

Confidence: medium

Additional weak hits (2):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.43 out of 29.38

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.00 out of 24.78

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

DW860_RS11660: X

AMP-binding (9..413): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FEFAFDVSVKDSWAIFSGEVNVYGPTEITCNCTY
Nearest Stachelhaus code(s):: Ser DVNEISVIEK (35% 8Å match)
Stachelhaus code match:: 60% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria