LOCUS       NZ_CAKMXI010000036      4348 bp    DNA     linear   CON 03-JUN-2024
DEFINITION  Streptomyces atroolivaceus strain TUE2108, whole genome shotgun
            sequence.
ACCESSION   NZ_CAKMXI010000036
VERSION     NZ_CAKMXI010000036.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces atroolivaceus
  ORGANISM  Streptomyces atroolivaceus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Wibberg,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2022) CEBITEC, Genome Research of Industrial
            Microorganisms (GRIM), Universitaetsstrasse 27, 33615, NRW, Germany
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_928722305.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 04:23:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,057
            CDSs (total)                      :: 6,983
            Genes (coding)                    :: 6,683
            CDSs (with protein)               :: 6,683
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 300
            CDSs (without protein)            :: 300
            Pseudo Genes (ambiguous residues) :: 0 of 300
            Pseudo Genes (frameshifted)       :: 123 of 300
            Pseudo Genes (incomplete)         :: 254 of 300
            Pseudo Genes (internal stop)      :: 20 of 300
            Pseudo Genes (multiple problems)  :: 87 of 300
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:59:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4348
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CAKMXI010000036.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..4346)
                     /NRPS_PKS="Domain: PKS_AT (25-329). E-value: 3.3e-102.
                     Score: 333.8. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35240_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (397-575). E-value: 4.8e-57.
                     Score: 184.9. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35240_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (875-1052). E-value: 1.7e-62.
                     Score: 202.6. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35240_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (1152-1223). E-value: 3.8e-30.
                     Score: 96.0. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35240_ACP.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /locus_tag="NBY68_RS35240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_250294489.1"
                     /sec_met_domain="PKS_AT (E-value: 5.5e-104, bitscore:
                     333.8, seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 5.3e-67, bitscore:
                     211.3, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.7e-16, bitscore:
                     46.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 6.8e-100, bitscore:
                     320.9, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 9.5e-75, bitscore:
                     237.5, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLADKGTAGRHLVRGTARPGARTAFVFAGQGAQWAGMGGQWYEQL
                     SAFATAFDAICVAADPQLEVPLADVVLAGSAGRPADHADLIHRTGYTQIALFAVEVALF
                     RTLEAWGVRPAVVLGHSIGEIAAAHVAGVLSLQDAVTLAVARGALMQQLPDDGAMLALD
                     ATPAQADAWLRALPEQHRRKVAIAAVNGPSAVVVSGASDAVDQVAEAAALAGRRLRRLQ
                     VSHAFHSPLMEPMLDEFRSVLARLTFSRPAVPMVSTVTGELAADDITSPDYWMRHAREA
                     VRFQDAVRVAESRVDAFLEIGPHTVLSRALEDSLSEGRSATVTSVAHRERPLVATLLRA
                     VGALWAGGAEVDWRALLPAVPADQDLPTYPFERQRFWPRVPPHRAGDVSGAGLAGLDHP
                     LLGAAVSVADGSSAGGAVLTGRLSLATHPWLADHAILGAVLLPGTAFVELALRAGQNVG
                     CGRIRDLTLETPLVLTPEDAMQIQVGVGPADEEGHRHVVVHSRLEPSDNETNGVETEWV
                     RHARGVVAPPDQGPDADGFGAVTGAVWPPRGAARVEVEDFYPALAELGYAYGPTFQGLR
                     KAWRRGTDLFADVELPEAAADDVAGFGVHPALLDAALHGVGLGGVTDDAENTSRLPFAW
                     GGVRLWASGARTVRVCLTTVGTAAVRVRIADATGTPVVGIDELVLRPVSETPVGPRRAL
                     VAESLFTEAWEPRKAGVQDAAVEAAPQVWGPDTGLDELTPADTVLLDCAGGGDTDALER
                     VRAHVGQALERIHAWLAYDGIVPARLTVLTRGAVEVHPGEGADPAQAAVHGLVRSACSE
                     HPERFALLDLAPGTGPGDVPWPAVRAALADQETEIAVRGGGVFVRRLVRTAPDADAPAA
                     YAGRGTVLITGGTGVIGSAVARHLVTAHGVRDLVLAGRRGADAPGATELAEELRAAGAE
                     VRLAACDVSQRSALAALVEPLTELRGVVHAAGVLDDGMVTAQTAERLDTVLRPKTDAAW
                     HLHELTRERDLDLFVLFSSAAGIFGSPGQANYAAANAFLDALARHRRAAGLPAHSLAWG
                     LWADLSTMTGKLSDTDMGRLGRGGLRAMSADEGLALFDASVRLEEPVAVPVRLDLTGRA
                     DSPALLRSLVRSAPRRVSAVPAAASSAWHERLLGLSATERSAALVDLVREHVATVLGHT
                     TAEDIAPGQSFDALGFDSLTAVELRNRLAEFTEVQLPTTLVFDYPSPEALAGFLGEALA
                     DPAERGARLDQVRATGDDPVAIVGMACRYPGGVGSPEELWDMVAGGREGIGGFPDDRGW
                     DLQRLYGREPGRTGSTYVRESGFLYEAADFDAGFFGVSPREATTMDPQHRLLLETSWEA
                     IERAGIDPTSLRRSRTGVYAGLMHHDYVARLQEIPEEIAGYLSNGTAGSVASGRIAYTF
                     GFEGPAVTVDTACSSSLVALDMAVSALRSGVCDLALAGGVAVMSTPT"
     source          1..4348
                     /db_xref="taxon:66869"
                     /mol_type="genomic DNA"
                     /note="contig: scaffold00036"
                     /organism="Streptomyces atroolivaceus"
                     /strain="TUE2108"
     gene            complement(<1..>4348)
                     /locus_tag="NBY68_RS35240"
     region          1..4348
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..4348
                     /SMILES="C=CC(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..4348
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<0:4346](-)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..4346)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS_motif       complement(83..130)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35240_0010"
                     /evalue="6.90E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="NBY68_RS35240"
                     /protein_end="1421"
                     /protein_start="1405"
                     /score="21.1"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       complement(158..187)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35240_0009"
                     /evalue="2.70E+01"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="NBY68_RS35240"
                     /protein_end="1396"
                     /protein_start="1386"
                     /score="-1.1"
                     /tool="antismash"
                     /translation="NGTAGSVASG"
     aSDomain        complement(677..889)
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35240_ACP.1"
                     /evalue="3.80E-30"
                     /label="NBY68_RS35240_ACP.1"
                     /locus_tag="NBY68_RS35240"
                     /protein_end="1223"
                     /protein_start="1152"
                     /score="96.0"
                     /tool="antismash"
                     /translation="DLVREHVATVLGHTTAEDIAPGQSFDALGFDSLTAVELRNRLAEF
                     TEVQLPTTLVFDYPSPEALAGFLGEA"
     aSModule        complement(677..4270)
                     /complete
                     /domains="nrpspksdomains_NBY68_RS35240_PKS_AT.1"
                     /domains="nrpspksdomains_NBY68_RS35240_PKS_DH.1"
                     /domains="nrpspksdomains_NBY68_RS35240_PKS_KR.1"
                     /domains="nrpspksdomains_NBY68_RS35240_ACP.1"
                     /locus_tags="NBY68_RS35240"
                     /monomer_pairings="mal -> ccmal"
                     /starter_module
                     /tool="antismash"
                     /type="pks"
     aSDomain        complement(1190..1720)
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N found: True"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35240_PKS_KR.1"
                     /evalue="1.70E-62"
                     /label="NBY68_RS35240_PKS_KR.1"
                     /locus_tag="NBY68_RS35240"
                     /protein_end="1052"
                     /protein_start="875"
                     /score="202.6"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="GTVLITGGTGVIGSAVARHLVTAHGVRDLVLAGRRGADAPGATEL
                     AEELRAAGAEVRLAACDVSQRSALAALVEPLTELRGVVHAAGVLDDGMVTAQTAERLDT
                     VLRPKTDAAWHLHELTRERDLDLFVLFSSAAGIFGSPGQANYAAANAFLDALARHRRAA
                     GLPAHSLAWGLWAD"
     CDS_motif       complement(1238..1348)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35240_0008"
                     /evalue="3.00E-24"
                     /label="PKSI-KR_m4"
                     /locus_tag="NBY68_RS35240"
                     /protein_end="1036"
                     /protein_start="999"
                     /score="77.7"
                     /tool="antismash"
                     /translation="LDLFVLFSSAAGIFGSPGQANYAAANAFLDALARHRR"
     CDS_motif       complement(1616..1717)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35240_0007"
                     /evalue="3.10E-08"
                     /label="PKSI-KR_m1"
                     /locus_tag="NBY68_RS35240"
                     /protein_end="910"
                     /protein_start="876"
                     /score="26.2"
                     /tool="antismash"
                     /translation="TVLITGGTGVIGSAVARHLVTAHGVRDLVLAGRR"
     aSDomain        complement(2621..3154)
                     /ASF="catalytic triad H,G,P found: False"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35240_PKS_DH.1"
                     /evalue="4.80E-57"
                     /label="NBY68_RS35240_PKS_DH.1"
                     /locus_tag="NBY68_RS35240"
                     /protein_end="575"
                     /protein_start="397"
                     /score="184.9"
                     /tool="antismash"
                     /translation="HPLLGAAVSVADGSSAGGAVLTGRLSLATHPWLADHAILGAVLLP
                     GTAFVELALRAGQNVGCGRIRDLTLETPLVLTPEDAMQIQVGVGPADEEGHRHVVVHSR
                     LEPSDNETNGVETEWVRHARGVVAPPDQGPDADGFGAVTGAVWPPRGAARVEVEDFYPA
                     LAELGYAYGPTFQGL"
     CDS_motif       complement(2780..2800)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35240_0006"
                     /evalue="1.00E+01"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="NBY68_RS35240"
                     /protein_end="522"
                     /protein_start="515"
                     /score="0.4"
                     /tool="antismash"
                     /translation="WVRHARG"
     CDS_motif       complement(2987..3061)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35240_0005"
                     /evalue="7.10E-10"
                     /label="PKSI-DH_m2"
                     /locus_tag="NBY68_RS35240"
                     /protein_end="453"
                     /protein_start="428"
                     /score="31.3"
                     /tool="antismash"
                     /translation="WLADHAILGAVLLPGTAFVELALRA"
     aSDomain        complement(3359..4270)
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35240_PKS_AT.1"
                     /evalue="3.30E-102"
                     /label="NBY68_RS35240_PKS_AT.1"
                     /locus_tag="NBY68_RS35240"
                     /protein_end="329"
                     /protein_start="25"
                     /score="333.8"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="VFAGQGAQWAGMGGQWYEQLSAFATAFDAICVAADPQLEVPLADV
                     VLAGSAGRPADHADLIHRTGYTQIALFAVEVALFRTLEAWGVRPAVVLGHSIGEIAAAH
                     VAGVLSLQDAVTLAVARGALMQQLPDDGAMLALDATPAQADAWLRALPEQHRRKVAIAA
                     VNGPSAVVVSGASDAVDQVAEAAALAGRRLRRLQVSHAFHSPLMEPMLDEFRSVLARLT
                     FSRPAVPMVSTVTGELAADDITSPDYWMRHAREAVRFQDAVRVAESRVDAFLEIGPHTV
                     LSRALEDSLSEGRSATVTSVAHR"
     CDS_motif       complement(3494..3529)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35240_0004"
                     /evalue="2.60E-04"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="NBY68_RS35240"
                     /protein_end="284"
                     /protein_start="272"
                     /score="14.4"
                     /tool="antismash"
                     /translation="YWMRHAREAVRF"
     CDS_motif       complement(3749..3787)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35240_0003"
                     /evalue="1.10E-03"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="NBY68_RS35240"
                     /protein_end="199"
                     /protein_start="186"
                     /score="12.3"
                     /tool="antismash"
                     /translation="AAVNGPSAVVVSG"
     CDS_motif       complement(3893..4015)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35240_0002"
                     /evalue="7.30E-22"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="NBY68_RS35240"
                     /protein_end="151"
                     /protein_start="110"
                     /score="69.6"
                     /tool="antismash"
                     /translation="GVRPAVVLGHSIGEIAAAHVAGVLSLQDAVTLAVARGALMQ"
     CDS_motif       complement(4226..4273)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35240_0001"
                     /evalue="2.20E-05"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="NBY68_RS35240"
                     /protein_end="40"
                     /protein_start="24"
                     /score="16.9"
                     /tool="antismash"
                     /translation="FVFAGQGAQWAGMGGQ"
ORIGIN
        1 ggtgggggtc gacatcacgg cgacgccgcc cgcgagtgcc aggtcgcaca cgccgctgcg
       61 cagcgcggac acggccatgt ccagggcgac gagggatgag gaacaggcgg tgtcgaccgt
      121 cacggcaggg ccctcgaagc cgaaggtgta ggcgatgcgg ccggaggcga cactgcccgc
      181 ggtgccgttg ctgagatacc cggcgatctc ctcggggatc tcctggagcc gtgccacgta
      241 gtcgtggtgc atcaggcccg cgtacacgcc ggtcctgctg cgccgcaggc tcgtcgggtc
      301 gatgcccgct cgctcgatgg cctcccagga ggtttcgagg agcagccggt gctgcgggtc
      361 catcgtggtg gcctcgcgcg gcgagacacc gaagaacccc gcgtcgaagt cggctgcctc
      421 gtacaggaat ccgctctcgc ggacgtacgt cgatccggtg cggccgggct ctcggccgta
      481 gagccgctgg aggtcccagc cgcggtcgtc gggaaagccg ccgatgccct cgcggccgcc
      541 ggcgaccatg tcccacagct cctcgggcga gccgacgccg ccaggatagc ggcaggccat
      601 gccgacgatg gcgacggggt cgtcgccggt ggcccgcacc tggtcgaggc gtgcgccccg
      661 ctccgcggga tccgcaaggg cctcgcccag gaatccggcg agcgcctcgg ggctcgggta
      721 gtcgaagacg agggtggtgg gcagctggac ttcggtgaac tcagccaggc ggttgcgcag
      781 ttcgacggcg gtcagcgagt cgaagccgag ggcgtcgaag gactggccgg gcgcgatgtc
      841 ctcggccgtg gtgtggccga ggacggtggc gacgtgctcg cgcaccaggt ccacgagggc
      901 ggcggagcgc tccgtcgccg acagaccgag cagcctctcg tgccatgccg aggaggcggc
      961 cgcggggacg gcggagacac ggcgcggggc ggacctgacc agggagcgca gcagcgccgg
     1021 cgaatcggcc cggccggtca ggtccaggcg tacgggaaca gcgaccggct cttcgagccg
     1081 caccgacgcg tcgaacaggg cgaggccctc gtcggcgctc atggcgcgga ggccgccccg
     1141 gccgagcctg cccatgtcgg tgtcgctgag cttccccgtc atggtgctga gatcggccca
     1201 caggccccac gccagcgagt gcgcgggcag ccccgccgcg cgtcggtgcc gggcgagcgc
     1261 gtccaggaag gcgttggcgg ccgcgtagtt ggcctggcca ggggagccga agatgcctgc
     1321 ggccgaggag aacaggacga acaggtcgag gtcccgctcc cgggtgagct cgtgcaggtg
     1381 ccaggcggcg tccgtcttgg gccgcagcac ggtgtccaga cgctcggcgg tctgcgcggt
     1441 gaccatgccg tcgtcgagga cgcctgcggc gtgcaccaca ccgcgcagct cggtcagggg
     1501 ttcgacgagt gcggccagcg cggaccgctg cgagacgtcg caggcggcga ggcgcacctc
     1561 ggcgcccgcc gcgcgcagct cctcggcgag ttcggtggct cccggtgcgt cggcacctcg
     1621 ccgcccggcg agcaccaggt cgcggacgcc gtgggcggtg accaggtggc gggccacggc
     1681 ggacccgatg acgccggtgc cgccggtgat caggacagtg ccccgcccgg cgtacgcggc
     1741 cggagcgtcg gcatcgggtg cggtgcgcac cagacgccgc acgaagaccc cgccgccacg
     1801 cacggcgatc tcggtctcct ggtcggcgag cgcggcgcgc acagcgggcc aagggacgtc
     1861 gccgggcccg gtgccggggg ccaggtcgag cagcgcgaac ctctcggggt gctcggagca
     1921 cgccgagcgc accagaccgt gcaccgcggc ctgggccggg tcggcgccct ctccggggtg
     1981 cacttcgacg gcgcccctgg tcagtacggt cagccgggcc gggacgatgc cgtcgtacgc
     2041 aagccaggca tggatgcgct ccagggcctg gcccacgtgg gctcgtaccc tttcgagggc
     2101 gtcggtgtcg ccacctcccg cacagtccag gagcacggtg tcggcgggcg tcagctcatc
     2161 gagaccggtg tccgggcccc agacctgcgg agctgcttca acggccgcgt cctgtacccc
     2221 cgccttccgg ggttcccacg cttcggtgaa cagggactct gcgaccaggg cgcgcctcgg
     2281 gcccacgggg gtctccgaga cgggccgcag cacgagttcg tcgatgccga cgacgggggt
     2341 gccggtcgcg tcggcgatcc gtacgcgtac tgcggcggta cccacggtcg tcagacagac
     2401 gcggacggtg cgggcgccgc tcgcccacag ccgcacacca ccccaggcga acggcagccg
     2461 cgaggtgttc tcggcatcgt cggtcacgcc gccgaggccg accccgtgca gggcggcatc
     2521 cagcagggcc gggtggacgc cgaatcccgc gacgtcgtcc gccgcggcct cgggcagctc
     2581 cacgtcggcg aagaggtcgg tgccgcggcg ccaggccttg cgcagcccct ggaaggtggg
     2641 gccgtaggcg tagcccagct cggcaagggc ggggtagaag tcctcgacct cgacccgggc
     2701 cgcaccgcgg ggcggccaca ccgcgccggt gaccgcgccg aagccgtcgg cgtccgggcc
     2761 ctggtccgga ggagcgacca cgccccgggc gtgccggacc cactcggtct cgacgccatt
     2821 cgtttcgttg tcggacggtt cgaggcgcga gtgcacgacg acgtggcggt ggccctcctc
     2881 gtcggcgggg cccacgccga cctggatctg catggcgtcc tcgggggtga ggacgagcgg
     2941 ggtctccagc gtgaggtcgc ggatccggcc gcagcccacg ttctggcccg cgcgcagggc
     3001 gagttcgacg aaggcggtgc ccggaaggag cacggcgccc aagatggcgt ggtcggcgag
     3061 ccaggggtgg gtggcgagcg agagccggcc ggtcagcacg gcgccacccg cggagctgcc
     3121 gtcggcgacg ctgacggctg caccgagcag cgggtggtcg agtccggcga gtccggcgcc
     3181 gctcacgtcg cccgcgcggt gcggcgggac gcggggccag aagcgctgcc gttcgaaggg
     3241 gtacgtgggc aggtcctggt cggcgggtac ggcgggcagc agcgcgcgcc agtccacttc
     3301 ggcgcccccg gcccacaggg cgccgaccgc cctcagcaga gtggcgacca ggggccgttc
     3361 gcggtgggcg acggaggtga cggtggcgga gcggccctcg gagaggctgt cctccagggc
     3421 ccgggacagc acggtgtgcg ggccgatctc caggaatgcg tccacgcgcg actccgcgac
     3481 gcgcacggcg tcctggaagc ggacggcttc gcgggcgtgc cgcatccagt agtccggcga
     3541 tgtgatgtcg tccgccgcga gttcaccggt gacggtggag accatcggga cggccgggcg
     3601 ggagaaggtg aggcgcgcaa gcacggagcg gaactcgtcg agcatcggct ccatgagcgg
     3661 cgagtggaag gcgtggctga cctggagcct gcgaagacgc cgccctgcga gcgcggcggc
     3721 ctcggccacc tggtcgacgg cgtccgaggc accggacacg accacggcgg aggggccgtt
     3781 gaccgcggcg atcgctacct tccgccggtg ctgctcgggc agggcgcgca gccatgcgtc
     3841 ggcctgtgcc ggggtggcgt cgagggccag catggcgccg tcgtccggta gttgctgcat
     3901 gagggcgccg cgggcgacgg ccagcgtgac ggcgtcctgg agggagagca ctccggccac
     3961 atgggcggcg gcgatctcac cgatggagtg accgaggacg acggccgggc gtacgcccca
     4021 tgcctccagg gtgcggaaca gcgcgacctc gacggcgaag agggcgatct gggtgtaacc
     4081 ggtccggtgg atcaggtccg cgtggtccgc ggggcggcct gcggagccgg caagcaccac
     4141 gtccgccagc ggcacctcga gctgagggtc ggcggctaca cagatggcgt cgaacgccgt
     4201 cgcgaacgcg gaaagttgct cgtaccactg ccctcccatg cccgcccact gcgcgccctg
     4261 ccccgcgaag acgaaggcag tcctggcgcc gggccgtgcc gtgccacgga cgaggtggcg
     4321 gcccgcggtg cccttgtcgg cgagggta
//
