LOCUS       NZ_CAKMXI010000033      6326 bp    DNA     linear   CON 03-JUN-2024
DEFINITION  Streptomyces atroolivaceus strain TUE2108, whole genome shotgun
            sequence.
ACCESSION   NZ_CAKMXI010000033
VERSION     NZ_CAKMXI010000033.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces atroolivaceus
  ORGANISM  Streptomyces atroolivaceus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Wibberg,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2022) CEBITEC, Genome Research of Industrial
            Microorganisms (GRIM), Universitaetsstrasse 27, 33615, NRW, Germany
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_928722305.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 04:23:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,057
            CDSs (total)                      :: 6,983
            Genes (coding)                    :: 6,683
            CDSs (with protein)               :: 6,683
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 300
            CDSs (without protein)            :: 300
            Pseudo Genes (ambiguous residues) :: 0 of 300
            Pseudo Genes (frameshifted)       :: 123 of 300
            Pseudo Genes (incomplete)         :: 254 of 300
            Pseudo Genes (internal stop)      :: 20 of 300
            Pseudo Genes (multiple problems)  :: 87 of 300
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:59:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6326
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CAKMXI010000033.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..2240
                     /locus_tag="NBY68_RS35210"
     source          1..6326
                     /db_xref="taxon:66869"
                     /mol_type="genomic DNA"
                     /note="contig: scaffold00033"
                     /organism="Streptomyces atroolivaceus"
                     /strain="TUE2108"
     region          1..6326
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..6326
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6326
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[2326:>4703](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2327..>4703
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS             <5..2240
                     /NRPS_PKS="Domain: PKS_KR (303-480). E-value: 9.2e-54.
                     Score: 174.1. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35210_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (586-657). E-value: 3.6e-30. Score:
                     96.1. Matches aSDomain: nrpspksdomains_NBY68_RS35210_ACP.1"
                     /NRPS_PKS="Domain: PKS_Docking_Cterm (674-744). E-value:
                     2.3e-12. Score: 39.6. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35210_PKS_Docking_Cterm.1"
                     /codon_start=3
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /locus_tag="NBY68_RS35210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_250294484.1"
                     /sec_met_domain="adh_short (E-value: 8.2e-59, bitscore:
                     186.2, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 2.6e-08,
                     bitscore: 21.0, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.3e-17, bitscore:
                     50.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="RRVGLPTYAFQTKHYWFDTAPARPEASADADPHAAFWEVVEREDL
                     EGLAKTLRLDEAQTGLTAVLPALSAWRRRQAEAISTESWRYQAHWKSLPAGPKAALSGT
                     WLVAVAPDQLDGALYETVTGALRAQGAVVEPLVVSGAEDAGQGERPWAEVLAAHSAAAG
                     VLSLLAQTESPLADASATPAGLAQNLALLQQLEAADSDAALWCVTQGAVSIGDRDPLTS
                     PAQALVWGLGRVAAQELPTRWGGLVDIPPEPDGRALTRLCEVLAAEADEDQVAVRSTAT
                     YGRRLVRAPLGDALGDGWTPGKGTVLITGGTGALGSEVARLLAARAEHLLIVSRQGRMA
                     PGATDLEAELVATGARVTMVACDVADSEALRHLLDSVPDDCPLTAVVHTAAVLDDGALT
                     TLTPEQLDRVLRVKAGAAHQLHEMTKDLGLTAFVLFSSLAGTVGMAGQGNYAPGNAYLD
                     ALAQYRRAQGLVATSVAWGSWARGGMAGRDVVGATSTRHGVPLLQPERALAGLETALAH
                     GDTSLVIADIDWKRFAYSYTAARPSHLLDELPEARAVLTRDEPTDAVATLTLSERLAGL
                     APAERERQLRTTVRTQVAAVLGHDSGETVELRRRFLELGLDSVTALELRNRLNTATGLR
                     LPTTIVFDCPTVHELVRYLGDALFGDEERTEGSKDAAALVTAELDQLEALLGQLPDGDP
                     SRVELSQRLRQLLRIASRPGVDGEEAVDAEDLESASNDDLFKYIGEEFGIS"
     aSDomain        915..1445
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35210_PKS_KR.1"
                     /evalue="9.20E-54"
                     /label="NBY68_RS35210_PKS_KR.1"
                     /locus_tag="NBY68_RS35210"
                     /protein_end="480"
                     /protein_start="303"
                     /score="174.1"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B2"
                     /tool="antismash"
                     /translation="GTVLITGGTGALGSEVARLLAARAEHLLIVSRQGRMAPGATDLEA
                     ELVATGARVTMVACDVADSEALRHLLDSVPDDCPLTAVVHTAAVLDDGALTTLTPEQLD
                     RVLRVKAGAAHQLHEMTKDLGLTAFVLFSSLAGTVGMAGQGNYAPGNAYLDALAQYRRA
                     QGLVATSVAWGSWA"
     aSModule        915..1976
                     /domains="nrpspksdomains_NBY68_RS35210_PKS_KR.1"
                     /domains="nrpspksdomains_NBY68_RS35210_ACP.1"
                     /incomplete
                     /locus_tags="NBY68_RS35210"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       918..1010
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35210_0001"
                     /evalue="5.40E-10"
                     /label="PKSI-KR_m1"
                     /locus_tag="NBY68_RS35210"
                     /protein_end="335"
                     /protein_start="304"
                     /score="31.9"
                     /tool="antismash"
                     /translation="TVLITGGTGALGSEVARLLAARAEHLLIVSR"
     CDS_motif       1296..1400
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35210_0002"
                     /evalue="1.70E-19"
                     /label="PKSI-KR_m4"
                     /locus_tag="NBY68_RS35210"
                     /protein_end="465"
                     /protein_start="430"
                     /score="62.3"
                     /tool="antismash"
                     /translation="AFVLFSSLAGTVGMAGQGNYAPGNAYLDALAQYRR"
     aSDomain        1764..1976
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35210_ACP.1"
                     /evalue="3.60E-30"
                     /label="NBY68_RS35210_ACP.1"
                     /locus_tag="NBY68_RS35210"
                     /protein_end="657"
                     /protein_start="586"
                     /score="96.1"
                     /tool="antismash"
                     /translation="TTVRTQVAAVLGHDSGETVELRRRFLELGLDSVTALELRNRLNTA
                     TGLRLPTTIVFDCPTVHELVRYLGDA"
     aSDomain        2028..2237
                     /aSDomain="PKS_Docking_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35210_PKS_Docking_Cterm.
                     1"
                     /evalue="2.30E-12"
                     /label="NBY68_RS35210_PKS_Docking_Cterm.1"
                     /locus_tag="NBY68_RS35210"
                     /protein_end="744"
                     /protein_start="674"
                     /score="39.6"
                     /tool="antismash"
                     /translation="VTAELDQLEALLGQLPDGDPSRVELSQRLRQLLRIASRPGVDGEE
                     AVDAEDLESASNDDLFKYIGEEFGI"
     gene            2327..>4703
                     /locus_tag="NBY68_RS35215"
     CDS             2327..>4703
                     /NRPS_PKS="Domain: PKS_Docking_Nterm (4-30). E-value:
                     2.5e-10. Score: 31.7. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35215_PKS_Docking_Nterm.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (36-461). E-value:
                     8.1e-183. Score: 600.1. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35215_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (573-789). E-value: 2.6e-75.
                     Score: 245.5. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35215_PKS_AT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_121825819.1"
                     /locus_tag="NBY68_RS35215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_250294485.1"
                     /sec_met_domain="mod_KS (E-value: 4e-228, bitscore: 745.0,
                     seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 2.6e-91, bitscore:
                     293.3, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.3e-76, bitscore: 245.5,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDDAKKLRYYLTRVTAELKETQSRLQAAESVDSEPMAIIGMSCRF
                     PGGVASPEDLWRLLSEGGDAITGFPTDRGWDAASLVDVTGDRPGSSYVAEGGFLHDAGL
                     FDADFFGISPREALAMDPQQRILLETSWELFERSGIAADALEGSRTGVFIGSSFRDYGS
                     RLPAIPEEVEGHAMTGTAGSVASGRISYSFGLTGPAVTVDTACSSSLVALHLAIQALRQ
                     GECSMALAGGVAIMSTPDLFTEFSRQQGLARDGRCKAFAASADGMSAAEGVGLLLVERL
                     SDARRNGHQVLAVIRGSAVNQDGASSGLTAPNGPAQQRVIRQALTSAGVKGSEVDVVEA
                     HGTGTALGDPIEAQALLATYGQDRLEDRPLLLGSLKSNIGHTQAAAGVAGVIKMVESMR
                     HGVVPRTLHVDEPTPHVDWSSGAVALVTEDCAWPETGRVRRAGVSSFGVSGTNAHVIVE
                     QAPVEEPAAVPDGEPGQVGVVAGGGVVPWALSGRSVPALREQAARLREFMTSSDVSLAD
                     VGLSLVSSRAGLEHRAVVLGSDREELLAGLEAVAAGESAEEVVSGVRTDRADGAVFVFP
                     GQGAQWAGMAVELLDSSPVFAERFGECVAGLEPHIDWVPEDVLREREGAPSLDRVDVVQ
                     PMLWAVMVSLAQVWRAHGVEPAAVVGHSQGELAAAVVAGVLSLGDAARIVALRSQLIGR
                     VLAGRGGMVSLPLAVEAASELIGPWSGRVSVATVNGPRSTVVAGEVVALDELMAVCERD
                     GVRARRVPVDYGSHSPQVELLRDALLELAASVVPRSGDV"
     aSDomain        2339..2416
                     /aSDomain="PKS_Docking_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35215_PKS_Docking_Nterm.
                     1"
                     /evalue="2.50E-10"
                     /label="NBY68_RS35215_PKS_Docking_Nterm.1"
                     /locus_tag="NBY68_RS35215"
                     /protein_end="30"
                     /protein_start="4"
                     /score="31.7"
                     /tool="antismash"
                     /translation="KKLRYYLTRVTAELKETQSRLQAAES"
     aSDomain        2435..3709
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35215_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="8.10E-183"
                     /label="NBY68_RS35215_PKS_KS.1"
                     /locus_tag="NBY68_RS35215"
                     /protein_end="461"
                     /protein_start="36"
                     /score="600.1"
                     /tool="antismash"
                     /translation="AIIGMSCRFPGGVASPEDLWRLLSEGGDAITGFPTDRGWDAASLV
                     DVTGDRPGSSYVAEGGFLHDAGLFDADFFGISPREALAMDPQQRILLETSWELFERSGI
                     AADALEGSRTGVFIGSSFRDYGSRLPAIPEEVEGHAMTGTAGSVASGRISYSFGLTGPA
                     VTVDTACSSSLVALHLAIQALRQGECSMALAGGVAIMSTPDLFTEFSRQQGLARDGRCK
                     AFAASADGMSAAEGVGLLLVERLSDARRNGHQVLAVIRGSAVNQDGASSGLTAPNGPAQ
                     QRVIRQALTSAGVKGSEVDVVEAHGTGTALGDPIEAQALLATYGQDRLEDRPLLLGSLK
                     SNIGHTQAAAGVAGVIKMVESMRHGVVPRTLHVDEPTPHVDWSSGAVALVTEDCAWPET
                     GRVRRAGVSSFGVSGTNAHVIVEQAP"
     aSModule        2435..4693
                     /domains="nrpspksdomains_NBY68_RS35215_PKS_KS.1"
                     /domains="nrpspksdomains_NBY68_RS35215_PKS_AT.1"
                     /incomplete
                     /locus_tags="NBY68_RS35215"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       2831..2893
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35215_0001"
                     /evalue="1.10E+01"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="NBY68_RS35215"
                     /protein_end="189"
                     /protein_start="168"
                     /score="-0.7"
                     /tool="antismash"
                     /translation="PEEVEGHAMTGTAGSVASGRI"
     CDS_motif       2915..2962
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35215_0002"
                     /evalue="3.40E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="NBY68_RS35215"
                     /protein_end="212"
                     /protein_start="196"
                     /score="22.0"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       3338..3382
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35215_0003"
                     /evalue="1.30E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="NBY68_RS35215"
                     /protein_end="352"
                     /protein_start="337"
                     /score="13.9"
                     /tool="antismash"
                     /translation="VEAHGTGTALGDPIE"
     CDS_motif       3443..3487
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35215_0004"
                     /evalue="7.60E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="NBY68_RS35215"
                     /protein_end="387"
                     /protein_start="372"
                     /score="11.9"
                     /tool="antismash"
                     /translation="GSLKSNIGHTQAAAG"
     CDS_motif       3773..3913
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35215_0005"
                     /evalue="1.10E+01"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="NBY68_RS35215"
                     /protein_end="529"
                     /protein_start="482"
                     /score="-2.1"
                     /tool="antismash"
                     /translation="GVVPWALSGRSVPALREQAARLREFMTSSDVSLADVGLSLVSSRA
                     GL"
     CDS_motif       4040..4096
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35215_0006"
                     /evalue="4.50E-10"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="NBY68_RS35215"
                     /protein_end="590"
                     /protein_start="571"
                     /score="31.0"
                     /tool="antismash"
                     /translation="VFVFPGQGAQWAGMAVELL"
     aSDomain        4046..4693
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35215_PKS_AT.1"
                     /evalue="2.60E-75"
                     /label="NBY68_RS35215_PKS_AT.1"
                     /locus_tag="NBY68_RS35215"
                     /protein_end="789"
                     /protein_start="573"
                     /score="245.5"
                     /specificity="substrate consensus: mmal"
                     /specificity="PKS signature: Methylmalonyl-CoA"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="VFPGQGAQWAGMAVELLDSSPVFAERFGECVAGLEPHIDWVPEDV
                     LREREGAPSLDRVDVVQPMLWAVMVSLAQVWRAHGVEPAAVVGHSQGELAAAVVAGVLS
                     LGDAARIVALRSQLIGRVLAGRGGMVSLPLAVEAASELIGPWSGRVSVATVNGPRSTVV
                     AGEVVALDELMAVCERDGVRARRVPVDYGSHSPQVELLRDALLELAASVVPRS"
     CDS_motif       4208..4279
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35215_0007"
                     /evalue="1.50E-09"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="NBY68_RS35215"
                     /protein_end="651"
                     /protein_start="627"
                     /score="30.0"
                     /tool="antismash"
                     /translation="LDRVDVVQPMLWAVMVSLAQVWRA"
     CDS_motif       4283..4393
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35215_0008"
                     /evalue="1.40E-18"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="NBY68_RS35215"
                     /protein_end="689"
                     /protein_start="652"
                     /score="58.6"
                     /tool="antismash"
                     /translation="GVEPAAVVGHSQGELAAAVVAGVLSLGDAARIVALRS"
     CDS_motif       4502..4540
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35215_0009"
                     /evalue="6.30E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="NBY68_RS35215"
                     /protein_end="738"
                     /protein_start="725"
                     /score="13.0"
                     /tool="antismash"
                     /translation="ATVNGPRSTVVAG"
     CDS_motif       4526..4582
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35215_0010"
                     /evalue="1.40E+01"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="NBY68_RS35215"
                     /protein_end="752"
                     /protein_start="733"
                     /score="-1.0"
                     /tool="antismash"
                     /translation="TVVAGEVVALDELMAVCER"
     CDS_motif       4610..4642
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35215_0011"
                     /evalue="1.70E-03"
                     /label="PKSI-AT-mM_m6"
                     /locus_tag="NBY68_RS35215"
                     /protein_end="772"
                     /protein_start="761"
                     /score="11.3"
                     /tool="antismash"
                     /translation="VDYGSHSPQVE"
     assembly_gap    4704..5065
                     /estimated_length=362
                     /gap_type="unknown"
                     /linkage_evidence="unspecified"
     gene            <5066..>6326
                     /locus_tag="NBY68_RS35220"
     CDS             5070..>6326
                     /NRPS_PKS="Domain: PKS_DH (35-203). E-value: 2.7e-59.
                     Score: 192.2. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35220_PKS_DH.1"
                     /codon_start=3
                     /inference="COORDINATES: protein motif:HMM:NF026115.4"
                     /locus_tag="NBY68_RS35220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polyketide synthase dehydratase domain-containing
                     protein"
                     /protein_id="WP_250294486.1"
                     /transl_table=11
                     /translation="MRVGLPTYAFQTKHYWLDGRTGTVGVSVAGLSTLGHPLLAVGVAL
                     ADGNGHVSTGRISLHTHPWLADHVVMGRALVPGAAVLELMLRAGESVGTELLDELVLHS
                     PLVVPAGDAALDLQVAVETPDEQGRRAVRLHSRAYRPGQDDDPEWTCHAEGTLIAAPAE
                     ERSVQDTVWPPVGAEAVDIDGFYDRLAATGYGYGPVFRGIRAVWRRGEELFADVVLPEG
                     GARDAERFGVHPALVDAALQTRLVSLLEGQGDRLMPFSFAGARLHATGATAVRARLTPV
                     GDDALSVRLTDHAGLPVLTIETLTSRPLTADAIAGQSVDSLYEVNWAPLGSGPEQVEAD
                     GSTAVIGERLPGLDLPRHADLAALAESVRGGGAVPHTVFLPCPPPAADGAAAATADNTS
                     AAHADGAPAAVRALLASVLG"
     aSDomain        5177..5680
                     /ASF="catalytic triad H,G,P inconclusive"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35220_PKS_DH.1"
                     /evalue="2.70E-59"
                     /label="NBY68_RS35220_PKS_DH.1"
                     /locus_tag="NBY68_RS35220"
                     /protein_end="203"
                     /protein_start="35"
                     /score="192.2"
                     /tool="antismash"
                     /translation="HPLLAVGVALADGNGHVSTGRISLHTHPWLADHVVMGRALVPGAA
                     VLELMLRAGESVGTELLDELVLHSPLVVPAGDAALDLQVAVETPDEQGRRAVRLHSRAY
                     RPGQDDDPEWTCHAEGTLIAAPAEERSVQDTVWPPVGAEAVDIDGFYDRLAATGYGYGP
                     VFRGI"
     CDS_motif       5261..5335
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35220_0001"
                     /evalue="1.30E-09"
                     /label="PKSI-DH_m2"
                     /locus_tag="NBY68_RS35220"
                     /protein_end="88"
                     /protein_start="63"
                     /score="30.5"
                     /tool="antismash"
                     /translation="WLADHVVMGRALVPGAAVLELMLRA"
     misc_feature    5481..5483
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 cccggcgggt cgggctgccg acctacgcct tccagaccaa gcactactgg ttcgacaccg
       61 ctccggcccg ccccgaggcg agcgcggacg cggatccgca cgccgccttc tgggaggtgg
      121 tggagcgcga ggatctggag ggcctcgcga agaccctgcg gctggacgag gcacagaccg
      181 ggctgacggc cgtactgccc gcgctctccg cttggcgcag gcggcaggcg gaagcgatca
      241 gcacggagtc gtggcggtac caggcgcact ggaagtcgct gcccgcgggc cccaaggccg
      301 ccctgtccgg cacctggctg gtcgccgtcg ccccggacca gctcgacggc gcgctgtacg
      361 agacagtgac cggggccctg cgcgcacagg gggccgtggt cgaaccgctg gtcgtgagcg
      421 gcgccgagga cgcagggcag ggcgagcgtc cgtgggccga ggtcctggcc gctcactccg
      481 cggcagccgg cgtgctgtcg ctgttggcgc aaaccgagag tccgctcgcg gacgcatccg
      541 ccaccccggc cgggcttgcc cagaacctcg cgttgctaca gcagttggag gccgccgact
      601 cggacgccgc actgtggtgc gtcacccagg gcgcggtgtc catcggtgac cgggacccgc
      661 tcaccagccc ggcgcaggcg ctggtctggg gcctcggccg ggtggcggcc caggaactgc
      721 cgacccgctg gggcggtctg gtcgacatcc cgccggagcc ggacgggcgc gccctcacgc
      781 ggctgtgcga agtgctcgcg gccgaggcgg acgaggacca ggtcgcggtc cgctctaccg
      841 cgacctacgg gcgccggctc gtccgcgcgc ctctcggtga cgcactggga gacggctgga
      901 cgccgggcaa gggcacagta ctgatcacgg gcggcaccgg cgcgctcggc agcgaggtcg
      961 ccaggctgct ggctgctcgc gccgagcacc tgttgatcgt cagcaggcaa ggccgtatgg
     1021 ctcctggcgc caccgacctc gaagccgagc tggtcgccac cggagcccgc gtcaccatgg
     1081 tcgcctgcga cgtggcagac tcggaggcgt tgcgccacct gttggactcg gtgcccgacg
     1141 actgcccgct gaccgctgtg gtgcacaccg cggctgtgct ggacgacggg gcgctcacca
     1201 cgctgacgcc cgagcagctc gaccgggtgc tgcgggtcaa ggcaggtgcg gcacaccaac
     1261 tgcacgaaat gacgaaggac ttggggctta ccgccttcgt gctgttctca tcgctggcgg
     1321 gcacggtcgg catggcgggc cagggcaact acgctccggg caacgcctac ctggacgccc
     1381 tcgcccagta ccgtcgtgca caggggctcg tggccacctc cgtggcctgg ggttcctggg
     1441 ccaggggtgg catggcgggc cgggacgtgg tcggcgccac cagcacccgg cacggcgtgc
     1501 cgttgctcca gccggagcgc gccctcgccg gactggagac ggcgctcgcc cacggcgaca
     1561 ccagcctcgt gatcgcggac atcgactgga agaggttcgc gtactcctac accgcggcca
     1621 ggcccagcca tctgctcgac gagctgccgg aggcccgcgc ggtactcacg agggacgaac
     1681 ccaccgacgc ggtcgccact ctcacgctgt ccgagcggct ggccggcctc gcaccggccg
     1741 agcgcgaacg gcagttacgc acgacggtcc gtacgcaggt ggcagccgta ctcggccacg
     1801 actccgggga gacggtggaa ctccggcgcc ggttcctgga gctcggcctg gactcggtca
     1861 ccgccctgga actgcgcaac cggctcaaca cggcgaccgg tctgcgactg cccaccacga
     1921 tcgtgttcga ctgtccgacc gtgcacgaac tcgtgcgcta cctcggcgac gcactgttcg
     1981 gcgacgagga gaggacggag ggctccaagg acgcggccgc tctcgtcacg gccgaactcg
     2041 accagctcga ggcgctcctg gggcaactgc ccgatggaga cccctctaga gtcgaactgt
     2101 cgcaacgcct gcggcagttg ctgcgtatcg catcccgacc aggtgtggac ggcgaggaag
     2161 ccgtcgatgc cgaagacctg gaatccgcga gcaacgacga cctcttcaag tacatcggcg
     2221 aggagttcgg gatctcctga gtccctgatc gacgccgcgt ttccgacacg cgcctttccg
     2281 ctgggcgcac gccacacttc cgtctcactc tcgggggcac gcaagcatgg acgacgccaa
     2341 gaagctccgt tactacctga cccgggtcac agccgagctg aaggagaccc aatcgcggct
     2401 gcaggccgcg gagtcggtgg acagcgagcc gatggcgatc atcgggatga gctgccggtt
     2461 ccccggcggg gtggcctctc ccgaggacct gtggcggctg ctctccgagg gcggcgacgc
     2521 catcaccgga ttccccaccg accgcggctg ggacgcggca tccctggtgg acgtgaccgg
     2581 agaccgcccc ggcagctcgt acgtggccga gggcggattc ctgcatgacg ccgggctgtt
     2641 cgacgccgac ttcttcggga tctcgccgcg cgaggcgctc gccatggacc cgcagcagcg
     2701 gatcctgctg gagacctcct gggagctgtt cgagcgttcc ggcatcgccg cggacgcact
     2761 ggagggcagc aggaccggcg tcttcatcgg ctccagcttc cgcgactacg gcagcaggct
     2821 gcccgcgatc cccgaagagg tcgagggcca cgccatgacg ggcaccgcgg gcagtgtggc
     2881 ctcggggcgc atctcctaca gcttcggcct gaccggtccc gctgtcacgg tggacacggc
     2941 gtgctcctct tcgctggtgg cgctgcacct tgcgattcag gcgctgcgcc agggcgagtg
     3001 ctcgatggcg ctcgcgggcg gggtcgccat catgtcgacg cctgacctgt tcacggagtt
     3061 cagccggcag cagggcctcg cacgcgacgg ccgctgcaag gcgttcgccg cgtccgctga
     3121 cggcatgagc gctgccgagg gagtgggcct gctgctggtc gagcggctct cggacgcacg
     3181 gcgcaacggc catcaggttc tcgccgtgat ccgcggctcg gcggtcaacc aggacggcgc
     3241 ctccagcgga ctgaccgcgc ccaacggccc cgcgcaacag cgcgtgatcc ggcaggcgct
     3301 gacgagcgca ggggtgaagg gctccgaggt cgacgtcgtc gaggcgcacg gcaccggcac
     3361 agcactgggc gaccccatcg aggcgcaggc gctgctggcg acgtacggcc aggaccggct
     3421 cgaggaccgg ccactgctgc tgggctccct caaatccaac atcggtcaca cgcaggccgc
     3481 cgcaggcgtg gccggcgtga tcaagatggt cgagtcgatg cggcacggtg tggtgccgag
     3541 gacgttgcac gtggatgagc cgacgccgca cgtggactgg tcgtcgggtg cggtggcgtt
     3601 ggtgaccgag gattgtgcgt ggccggagac gggtcgggta cggcgtgcgg gtgtgtcgtc
     3661 gttcggtgtg agcgggacga acgcacacgt gatcgtggaa caggctcccg tggaggagcc
     3721 ggccgcggtc ccggatggcg agccggggca ggtcggtgtg gtggccggtg gcggtgtggt
     3781 gccgtgggcg ctttcgggcc ggtcggtccc ggcgctgcgc gaacaggcgg cgcggttgcg
     3841 ggagttcatg acgtcgtcgg acgtgtcgct cgcggatgtc ggtctgtcgt tggtgtcgtc
     3901 gcgggcgggg cttgagcacc gggcggtggt gctgggctcg gatcgtgagg agctgctggc
     3961 gggactggag gcggtggcgg cgggtgagtc cgccgaggag gtggtgtccg gggtacggac
     4021 cgacagggct gatggtgcgg tgttcgtgtt cccggggcag ggggcgcagt gggcgggtat
     4081 ggctgtggag ttgctggatt cgtcgccggt gttcgcggag cggttcggtg agtgtgtggc
     4141 ggggttggag ccgcacatcg actgggttcc ggaggatgtg ctgcgggagc gggagggtgc
     4201 gccgtcgttg gatcgggtgg atgtggtcca gccgatgttg tgggcggtca tggtgtccct
     4261 cgcgcaggtg tggcgtgctc acggggtgga gcctgcggct gtggtggggc attcgcaggg
     4321 tgagttggcg gctgcggtgg tggcgggggt gttgtcgttg ggggatgcgg cgcggatcgt
     4381 ggcgttgcgg agtcagctga tcgggcgggt gttggcgggt cggggtggca tggtgtcgtt
     4441 gccgttggcg gtggaggctg cgtcggagct gattggtccg tggtcgggtc gggtcagtgt
     4501 ggcgacggtg aacgggccgc gttcgacggt ggtggcgggt gaggttgtgg cgctggatga
     4561 gctgatggct gtgtgtgagc gtgatggggt gcgtgcgcgt cgggttccgg tggattacgg
     4621 gtcgcacagt ccgcaggtcg agctgttgag ggatgccctg ttggagttgg ctgcttcggt
     4681 ggtgccgcgt tcgggtgacg tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnncgcgc cgggtcgggc tgccgaccta cgccttccag
     5101 accaagcact actggctcga cggcaggacc ggcaccgtgg gggtgtccgt ggccgggctc
     5161 tccacgctcg gccaccccct gctggcggtc ggtgtggctc tcgcggacgg caacggccac
     5221 gtctccaccg ggcgcatctc cctgcacacc cacccctggc tggccgacca cgtggtgatg
     5281 ggccgggcgc tcgtgccggg ggccgccgta ttggagctga tgctgcgggc cggtgagagc
     5341 gtgggcaccg agctgctgga cgaactcgta ctgcactccc cgctggtcgt gccggccggt
     5401 gacgcggcgc tcgacctcca ggtggccgtc gagacccccg acgagcaggg ccggcgcgcg
     5461 gtgcggttgc actcccgggc ttatcgcccc gggcaggatg acgaccccga gtggacctgt
     5521 cacgcggaag gcacgctgat cgccgccccc gccgaggagc ggtccgtgca agacaccgtc
     5581 tggccgcctg tcggcgcgga ggccgtggac atcgacggtt tctacgaccg cctggcagcc
     5641 acgggatacg gctacggccc ggtcttccga ggcatccgtg cggtgtggcg gcgcggcgag
     5701 gaactgttcg ccgatgtcgt actgccggag ggcggcgcgc gggacgccga gcggttcggg
     5761 gtgcatcccg ccctcgtcga cgcggcgctc cagacgcgtc tggtgagtct gctggagggg
     5821 cagggcgacc ggctgatgcc gttctcgttc gccggggccc gtctccacgc gaccggtgcc
     5881 acagcggtcc gggcccggct gacgccggtg ggcgacgacg ccctttcggt ccggttgacc
     5941 gatcacgcgg ggctgccggt cctcaccatc gagaccctca cctcgcggcc actcaccgca
     6001 gacgcgatcg cgggccagag cgtcgactcg ctgtacgagg tcaactgggc gccgctgggc
     6061 agtggtccgg agcaggtcga agccgacgga tcgaccgcgg tcatcggtga aaggctgccg
     6121 ggcctcgacc tgccacggca cgcggacctc gccgcgctcg ccgagagcgt ccgcggcggc
     6181 ggagccgtac cgcacaccgt gttcctgccc tgcccgccac cggccgccga cggcgccgct
     6241 gccgctacag ccgacaacac ctctgccgcc catgccgacg gcgcccctgc cgccgtccgc
     6301 gcgctgctgg cctccgtgct gggcat
//
