LOCUS       NZ_CAKMXI010000032     10243 bp    DNA     linear   CON 03-JUN-2024
DEFINITION  Streptomyces atroolivaceus strain TUE2108, whole genome shotgun
            sequence.
ACCESSION   NZ_CAKMXI010000032
VERSION     NZ_CAKMXI010000032.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces atroolivaceus
  ORGANISM  Streptomyces atroolivaceus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Wibberg,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2022) CEBITEC, Genome Research of Industrial
            Microorganisms (GRIM), Universitaetsstrasse 27, 33615, NRW, Germany
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_928722305.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 04:23:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,057
            CDSs (total)                      :: 6,983
            Genes (coding)                    :: 6,683
            CDSs (with protein)               :: 6,683
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 300
            CDSs (without protein)            :: 300
            Pseudo Genes (ambiguous residues) :: 0 of 300
            Pseudo Genes (frameshifted)       :: 123 of 300
            Pseudo Genes (incomplete)         :: 254 of 300
            Pseudo Genes (internal stop)      :: 20 of 300
            Pseudo Genes (multiple problems)  :: 87 of 300
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:59:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10243
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CAKMXI010000032.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10243
                     /db_xref="taxon:66869"
                     /mol_type="genomic DNA"
                     /note="contig: scaffold00032"
                     /organism="Streptomyces atroolivaceus"
                     /strain="TUE2108"
     region          1..10243
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..10243
                     /SMILES="C(C)C(O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10243
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[15:>10243](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      16..>10243
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     gene            16..6210
                     /locus_tag="NBY68_RS35200"
     CDS             16..6210
                     /NRPS_PKS="Domain: PKS_KR (71-248). E-value: 1.1e-57.
                     Score: 186.9. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35200_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (338-422). E-value: 2e-29. Score:
                     93.8. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35200_PKS_PP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (449-873). E-value:
                     1.9e-178. Score: 585.6. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35200_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (983-1281). E-value: 1.7e-105.
                     Score: 344.6. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35200_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_KR (1624-1802). E-value: 1.2e-60.
                     Score: 196.5. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35200_PKS_KR.2"
                     /NRPS_PKS="Domain: ACP (1910-1981). E-value: 5.2e-28.
                     Score: 89.2. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35200_ACP.1"
                     /NRPS_PKS="Domain: PKS_Docking_Cterm (1990-2063). E-value:
                     4.6e-14. Score: 45.0. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35200_PKS_Docking_Cterm.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF045894.1"
                     /locus_tag="NBY68_RS35200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_250294482.1"
                     /sec_met_domain="adh_short (E-value: 2.6e-63, bitscore:
                     200.3, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 7e-220, bitscore: 717.2,
                     seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 4.3e-89, bitscore:
                     285.5, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 5.7e-107, bitscore:
                     344.6, seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.9e-16, bitscore:
                     47.6, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRYRAFDLGEAGADRLGEMLAHLAELFARGELVPLPVTAWDTRQA
                     PQAFRHMSQARHIGKVVLTNPRAALDGTVLVTGGTGVIGSAVARHLVTGHGVRDLVLTG
                     RRGQDAPGARELAAELTGLGATVRVEACDAADRAALGTLLDGLDGLCGVVHAAGALDDG
                     VLSALTPERLETVLRPKLDAAWNLHELTRGRDLALFVLFSSAAGVFGSPGQGGYAAANS
                     FLDALAQERRTQGLPAHSLAWGLWADRSAMTGALSTTDLDRMRRVGVRPLATEEGLALF
                     DAAVRLPDPHTVPVRLDTATLREQRPLAPLFRALVRTTAKRAAATTPAGSGDGLRERLA
                     ALAPTERDRELTELVRAQAALVLGHGTAGAVEAGRAFRDMGFDSLTAVELRNRIGSATG
                     LRLTVTAVFDHPTPAALAAEVAIRLGIDGDIGRERTTPTAAPAIAADDDPIVIIGMSCR
                     FPGHIDSPETLWQLLTDERDAMGDYPTDRGWDSVDLHNPEAPDLHFAKVGGFLHDMADF
                     DPGFFGMSPREAQATDPQQRLLLEATWEALERAGIDPATLRSTPTGIFTGLIYNDYAAR
                     FPDLLSGYEGYLGNGSANSVASGRIAYTFGLEGPAITVDTACSSSLVALHQAAHALRNG
                     DCTLAVAGGVTVMSTPRPMVEFSRISGLSPDGRCKAFSAEANGMGFAEGVGMLVVERLS
                     DARRNGHPVLAVLRGSALNQDGASNGLTAPSGPAQQRVIRQALANAGLTTADVDVVEAH
                     GTGTSLGDPIEAQAVLATYGKDRPEDKPLLLGSVKSNIGHTQAAAGVAGVIKMVLALRH
                     GVVPRTLHVDEPTPHIEWDSAGVRLADASVPWPETGRPRRGAVSSFGISGTNAHVILEH
                     APEPEPEPEPEPEPEAVEDTRDHLPWVISAKNPESLRKQALRLHAHVSAAPEATLVDIA
                     RSLLTTRAGFEHRAVVIGRDREELLRRLAAFGHGDETPGVIQGTAASDTRTAFVFPGQG
                     SQWAGMATELLDSSLAFARRFGECAAALAPHIDWEPADVLREREDAPSLERVDVIQPML
                     WAVMVSLAAVWRAHGVKPAAVAGHSQGELAAAVVAGVLSLQDAARIVALRSQLIGRELA
                     GQGGMVSVPVGEDAAEELIEPWAGRISVATINGPQSTVVAGEGPALDELMAACERDGVR
                     ARRIPVDYGSHTPQVEQLREALLELAAPVTPRPAQVPMYSTVTAEPLEDGMADAEYWYR
                     NLREPVRFQQTARALADAGHGVFVEVSPHPVLTTGIEASLADLDAAPVVTGTLRRDDGG
                     RSRLLTALAELYVAGAPVDWSSTFDGGRAVDLPTYAFHRQRYWLDAPAPAQGTADSGVD
                     SAFWTAVDGGDTESLSAALGVADDSVRSSLATVLPALSAWHGKQREASAVDAWRYGIEW
                     TPVQDSQARLTGTWLLAVPEERADDPAVREIGAGIERRGGSVRQVLLPAERAEDVATTG
                     ELPYVAEISVRLRAVEGHERAAGVLALTGRDEDSHPAHPGVPLGFTRTLGLIQALATTA
                     VTAPMWCVTSGAVTTGSSAELTAPSQAMVWGLGRVAAQEYPDRWGGLIDLPAEPGERDV
                     DRLCALLTGETGEDQLAVRPSGVRARRLVRTAASAGRDGAWQPPKGTILITGGTGALGG
                     HIARWLAERGAEHLMLLSRSGPDAEGADELRAQLSAAGARVTVLSCDAGDRDALAAALA
                     GIPEKLPLTAVFHTAAALDDGPLETLTLPRVAASLRSKAGAAWNLHELTADADLSAFVL
                     FSSTAGTFGAAGQGNYAPGNAYLDALAWYRRAHGQVAASIGWGAWAQGGLAEKDAVADL
                     RRRHGVPLMSPQRATLAMERALAGDDTFVVLADIEWDKFQLAYTAARPSPLLYGLAEVQ
                     RPQKAVDGADTAGTDGPSFAERLFGMARKEQVRMLREAVRTHAAAVLGHDRAESVPLTR
                     PFAELGLDSVTAVELRNRLGGVVGHKLPAALVFDHPTVADLVAYLHDLLFGDETYSSPM
                     LAELDRLDVELATLRDDDPVRGEVAERLEKLLRRVSPDSVVSSGGRAAQSWDLQAASHD
                     EVFAMIDDELGKP"
     CDS_motif       157..204
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0001"
                     /evalue="5.70E-07"
                     /label="PKSI-ER_m9"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="63"
                     /protein_start="47"
                     /score="22.2"
                     /tool="antismash"
                     /translation="AFRHMSQARHIGKVVL"
     aSDomain        229..759
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N found: True"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35200_PKS_KR.1"
                     /evalue="1.10E-57"
                     /label="NBY68_RS35200_PKS_KR.1"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="248"
                     /protein_start="71"
                     /score="186.9"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="GTVLVTGGTGVIGSAVARHLVTGHGVRDLVLTGRRGQDAPGAREL
                     AAELTGLGATVRVEACDAADRAALGTLLDGLDGLCGVVHAAGALDDGVLSALTPERLET
                     VLRPKLDAAWNLHELTRGRDLALFVLFSSAAGVFGSPGQGGYAAANSFLDALAQERRTQ
                     GLPAHSLAWGLWAD"
     aSModule        229..1281
                     /domains="nrpspksdomains_NBY68_RS35200_PKS_KR.1"
                     /domains="nrpspksdomains_NBY68_RS35200_PKS_PP.1"
                     /incomplete
                     /locus_tags="NBY68_RS35200"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       232..333
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0002"
                     /evalue="8.20E-08"
                     /label="PKSI-KR_m1"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="106"
                     /protein_start="72"
                     /score="24.9"
                     /tool="antismash"
                     /translation="TVLVTGGTGVIGSAVARHLVTGHGVRDLVLTGRR"
     CDS_motif       550..579
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0003"
                     /evalue="3.60E-02"
                     /label="PKSI-KR_m3"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="188"
                     /protein_start="178"
                     /score="7.8"
                     /tool="antismash"
                     /translation="PKLDAAWNLH"
     CDS_motif       601..711
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0004"
                     /evalue="3.20E-20"
                     /label="PKSI-KR_m4"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="232"
                     /protein_start="195"
                     /score="64.7"
                     /tool="antismash"
                     /translation="LALFVLFSSAAGVFGSPGQGGYAAANSFLDALAQERR"
     aSDomain        1030..1281
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35200_PKS_PP.1"
                     /evalue="2.00E-29"
                     /label="NBY68_RS35200_PKS_PP.1"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="422"
                     /protein_start="338"
                     /score="93.8"
                     /tool="antismash"
                     /translation="LAALAPTERDRELTELVRAQAALVLGHGTAGAVEAGRAFRDMGFD
                     SLTAVELRNRIGSATGLRLTVTAVFDHPTPAALAAEVAI"
     aSDomain        1363..2634
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35200_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="1.90E-178"
                     /label="NBY68_RS35200_PKS_KS.1"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="873"
                     /protein_start="449"
                     /score="585.6"
                     /tool="antismash"
                     /translation="IVIIGMSCRFPGHIDSPETLWQLLTDERDAMGDYPTDRGWDSVDL
                     HNPEAPDLHFAKVGGFLHDMADFDPGFFGMSPREAQATDPQQRLLLEATWEALERAGID
                     PATLRSTPTGIFTGLIYNDYAARFPDLLSGYEGYLGNGSANSVASGRIAYTFGLEGPAI
                     TVDTACSSSLVALHQAAHALRNGDCTLAVAGGVTVMSTPRPMVEFSRISGLSPDGRCKA
                     FSAEANGMGFAEGVGMLVVERLSDARRNGHPVLAVLRGSALNQDGASNGLTAPSGPAQQ
                     RVIRQALANAGLTTADVDVVEAHGTGTSLGDPIEAQAVLATYGKDRPEDKPLLLGSVKS
                     NIGHTQAAAGVAGVIKMVLALRHGVVPRTLHVDEPTPHIEWDSAGVRLADASVPWPETG
                     RPRRGAVSSFGISGTNAHVILEHAP"
     aSModule        1363..5958
                     /complete
                     /domains="nrpspksdomains_NBY68_RS35200_PKS_KS.1"
                     /domains="nrpspksdomains_NBY68_RS35200_PKS_AT.1"
                     /domains="nrpspksdomains_NBY68_RS35200_PKS_KR.2"
                     /domains="nrpspksdomains_NBY68_RS35200_ACP.1"
                     /locus_tags="NBY68_RS35200"
                     /monomer_pairings="mmal -> Me-ohmal"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       1783..1812
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0005"
                     /evalue="1.70E+01"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="599"
                     /protein_start="589"
                     /score="-0.5"
                     /tool="antismash"
                     /translation="NGSANSVASG"
     CDS_motif       1840..1887
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0006"
                     /evalue="1.10E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="624"
                     /protein_start="608"
                     /score="20.5"
                     /tool="antismash"
                     /translation="GPAITVDTACSSSLVA"
     CDS_motif       2263..2307
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0007"
                     /evalue="3.90E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="764"
                     /protein_start="749"
                     /score="12.6"
                     /tool="antismash"
                     /translation="VEAHGTGTSLGDPIE"
     CDS_motif       2368..2412
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0008"
                     /evalue="8.20E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="799"
                     /protein_start="784"
                     /score="11.9"
                     /tool="antismash"
                     /translation="GSVKSNIGHTQAAAG"
     CDS_motif       2962..3015
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0009"
                     /evalue="2.50E-09"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="1000"
                     /protein_start="982"
                     /score="28.8"
                     /tool="antismash"
                     /translation="FVFPGQGSQWAGMATELL"
     aSDomain        2965..3858
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35200_PKS_AT.1"
                     /evalue="1.70E-105"
                     /label="NBY68_RS35200_PKS_AT.1"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="1281"
                     /protein_start="983"
                     /score="344.6"
                     /specificity="substrate consensus: mmal"
                     /specificity="PKS signature: Methylmalonyl-CoA"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="VFPGQGSQWAGMATELLDSSLAFARRFGECAAALAPHIDWEPADV
                     LREREDAPSLERVDVIQPMLWAVMVSLAAVWRAHGVKPAAVAGHSQGELAAAVVAGVLS
                     LQDAARIVALRSQLIGRELAGQGGMVSVPVGEDAAEELIEPWAGRISVATINGPQSTVV
                     AGEGPALDELMAACERDGVRARRIPVDYGSHTPQVEQLREALLELAAPVTPRPAQVPMY
                     STVTAEPLEDGMADAEYWYRNLREPVRFQQTARALADAGHGVFVEVSPHPVLTTGIEAS
                     LADLDAAPVVTGTLRRD"
     CDS_motif       3127..3198
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0010"
                     /evalue="3.40E-09"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="1061"
                     /protein_start="1037"
                     /score="29.0"
                     /tool="antismash"
                     /translation="LERVDVIQPMLWAVMVSLAAVWRA"
     CDS_motif       3202..3321
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0011"
                     /evalue="3.00E-18"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="1102"
                     /protein_start="1062"
                     /score="58.0"
                     /tool="antismash"
                     /translation="GVKPAAVAGHSQGELAAAVVAGVLSLQDAARIVALRSQLI"
     CDS_motif       3421..3459
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0012"
                     /evalue="7.40E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="1148"
                     /protein_start="1135"
                     /score="12.8"
                     /tool="antismash"
                     /translation="ATINGPQSTVVAG"
     CDS_motif       3529..3561
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0013"
                     /evalue="5.80E-03"
                     /label="PKSI-AT-mM_m6"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="1182"
                     /protein_start="1171"
                     /score="9.7"
                     /tool="antismash"
                     /translation="VDYGSHTPQVE"
     CDS_motif       3670..3714
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0014"
                     /evalue="1.30E-05"
                     /label="PKSI-AT-mM_m8"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="1233"
                     /protein_start="1218"
                     /score="17.2"
                     /tool="antismash"
                     /translation="DAEYWYRNLREPVRF"
     CDS_motif       3757..3786
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0015"
                     /evalue="3.60E-03"
                     /label="PKSI-AT-mM_m9"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="1257"
                     /protein_start="1247"
                     /score="10.0"
                     /tool="antismash"
                     /translation="FVEVSPHPVL"
     aSDomain        4888..5421
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35200_PKS_KR.2"
                     /evalue="1.20E-60"
                     /label="NBY68_RS35200_PKS_KR.2"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="1802"
                     /protein_start="1624"
                     /score="196.5"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B2"
                     /tool="antismash"
                     /translation="GTILITGGTGALGGHIARWLAERGAEHLMLLSRSGPDAEGADELR
                     AQLSAAGARVTVLSCDAGDRDALAAALAGIPEKLPLTAVFHTAAALDDGPLETLTLPRV
                     AASLRSKAGAAWNLHELTADADLSAFVLFSSTAGTFGAAGQGNYAPGNAYLDALAWYRR
                     AHGQVAASIGWGAWA"
     CDS_motif       4891..4989
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0016"
                     /evalue="5.40E-15"
                     /label="PKSI-KR_m1"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="1658"
                     /protein_start="1625"
                     /score="47.9"
                     /tool="antismash"
                     /translation="TILITGGTGALGGHIARWLAERGAEHLMLLSRS"
     CDS_motif       5218..5244
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0017"
                     /evalue="1.80E+00"
                     /label="PKSI-KR_m3"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="1743"
                     /protein_start="1734"
                     /score="2.9"
                     /tool="antismash"
                     /translation="KAGAAWNLH"
     CDS_motif       5272..5376
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35200_0018"
                     /evalue="1.80E-17"
                     /label="PKSI-KR_m4"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="1787"
                     /protein_start="1752"
                     /score="55.9"
                     /tool="antismash"
                     /translation="AFVLFSSTAGTFGAAGQGNYAPGNAYLDALAWYRR"
     aSDomain        5746..5958
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35200_ACP.1"
                     /evalue="5.20E-28"
                     /label="NBY68_RS35200_ACP.1"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="1981"
                     /protein_start="1910"
                     /score="89.2"
                     /tool="antismash"
                     /translation="EAVRTHAAAVLGHDRAESVPLTRPFAELGLDSVTAVELRNRLGGV
                     VGHKLPAALVFDHPTVADLVAYLHDL"
     aSDomain        5986..6204
                     /aSDomain="PKS_Docking_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35200_PKS_Docking_Cterm.
                     1"
                     /evalue="4.60E-14"
                     /label="NBY68_RS35200_PKS_Docking_Cterm.1"
                     /locus_tag="NBY68_RS35200"
                     /protein_end="2063"
                     /protein_start="1990"
                     /score="45.0"
                     /tool="antismash"
                     /translation="PMLAELDRLDVELATLRDDDPVRGEVAERLEKLLRRVSPDSVVSS
                     GGRAAQSWDLQAASHDEVFAMIDDELGK"
     gene            6309..>10243
                     /locus_tag="NBY68_RS35205"
     CDS             6309..>10243
                     /NRPS_PKS="Domain: PKS_Docking_Nterm (3-31). E-value:
                     1e-13. Score: 42.6. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35205_PKS_Docking_Nterm.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (35-460). E-value:
                     4.4e-181. Score: 594.3. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35205_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (573-870). E-value: 1.4e-104.
                     Score: 341.6. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35205_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (938-1110). E-value: 6.8e-59.
                     Score: 190.9. Matches aSDomain:
                     nrpspksdomains_NBY68_RS35205_PKS_DH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014140392.1"
                     /locus_tag="NBY68_RS35205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_250294483.1"
                     /sec_met_domain="mod_KS (E-value: 9.3e-229, bitscore:
                     746.4, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 8.5e-92, bitscore:
                     294.3, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 4.7e-106, bitscore:
                     341.6, seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPNEEKLFDYLKRVTAELKETKSRLKAVESAGSEPVAIVGMSCRY
                     PGGVASPEDLWRLLSEGGDAITGLPADRGWDPSSLIDVTGERPGSSYVAEGGFLHDAGE
                     FDAGFFGISPREALAMDPQQRLLLETSWELFERSGINPTTLRGSDTGVFVGSSFHGYGD
                     PATAPDEAQGYLLTGRADSVISGRIAYTLGLEGPALTVDTACSSSLVALHLAINALQLE
                     ECSLALAGGVMVMSTPEVFVGLSRQQGLARDGRCKAFAASADGMSASEGVGLLLVERLS
                     DARRNGHQVLAVIRGSAVNQDGASNGLTAPHGPAQQRVIRQALESAGLVPAQVDVVEAH
                     GTGTKLGDPIEAQALLATYGQERDEEQPLLLGSLKSNIGHTQAAAGVASVIKMVEAMRH
                     GVVPRTLHVDEPTPHVDWTAGAVELVTEERPWPETGRARRAGVSSFGVSGTNAHVIVEQ
                     APAEEPPEAEAEAEAGPAGLVSGGGVVPWVLSGRSAAGLRGQAERLHRFVASSEASIAD
                     LGWSLASSRAGLGHRAVLLGADQEELVTALKALEEGEPAAGVVTGVASEGASDVVFVFP
                     GQGSQWAGMAVGLLDSSPVFAERFGECATALAPYVDWVPVDVLREREGAPSLDRVDVVQ
                     PMLWAVMVSLAAVWRASGVRPAAVVGHSQGELAAAVVAGVLSVEDGARIVALRSQLIGR
                     ELAGQGGMVSVPLAEGAAVELIESWAGRISVATVNGPRSTVVAGEAAALDELVAVCERD
                     GVRARRIPVDYGSHTPQVERLREALLELAAPVAPRPGDVPMYSTVTGALLEDGVADAEY
                     WFRNLRQPVRFLDTVEELIASGHTTFVEVSPHPVLTSSVEETGEVGGVEVCGVGTLRRD
                     QGGVRRFLTSLAELWVRGVEPEWQGVFAGSGAGRVGLPTYAFQRRHYWLDGAAGTAEVS
                     GAGLSALGHPLLAAGITIADGEGHLFTGRISLRTHSWLAGHAVLGRVLVPGAAVLELVL
                     RAGESVGCELLDELVLQAPLVVPAGDAAVDLQVAVDAPDEQGRRAVRLHSRAVRPGQED
                     DGAEWVCHAEGLLTAVPAASAGEQAAADATWPPAGAEAVDIDGFYDRLAATGYGYGPIF
                     QGIRAVWRRGEELFADVVLPEGGERDAGRFGVHPALVDAALQTGLVTLLEDGGERMMPF
                     SFAGVRLHATGATAARVRLTPTGADSLAVRLTDGTGLPVLTIDTLTSRPLTADTLSGQS
                     VDSLYEVNWALFTGGSGDITSLPGSAVVLGEGLAELGLPRHEDLAALAESVRGGGAVPA
                     TVLLPCPRTAAGDTPAAVRALLASVLGT"
     aSDomain        6318..6401
                     /aSDomain="PKS_Docking_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35205_PKS_Docking_Nterm.
                     1"
                     /evalue="1.00E-13"
                     /label="NBY68_RS35205_PKS_Docking_Nterm.1"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="31"
                     /protein_start="3"
                     /score="42.6"
                     /tool="antismash"
                     /translation="EEKLFDYLKRVTAELKETKSRLKAVESA"
     aSDomain        6414..7688
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35205_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="4.40E-181"
                     /label="NBY68_RS35205_PKS_KS.1"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="460"
                     /protein_start="35"
                     /score="594.3"
                     /tool="antismash"
                     /translation="VAIVGMSCRYPGGVASPEDLWRLLSEGGDAITGLPADRGWDPSSL
                     IDVTGERPGSSYVAEGGFLHDAGEFDAGFFGISPREALAMDPQQRLLLETSWELFERSG
                     INPTTLRGSDTGVFVGSSFHGYGDPATAPDEAQGYLLTGRADSVISGRIAYTLGLEGPA
                     LTVDTACSSSLVALHLAINALQLEECSLALAGGVMVMSTPEVFVGLSRQQGLARDGRCK
                     AFAASADGMSASEGVGLLLVERLSDARRNGHQVLAVIRGSAVNQDGASNGLTAPHGPAQ
                     QRVIRQALESAGLVPAQVDVVEAHGTGTKLGDPIEAQALLATYGQERDEEQPLLLGSLK
                     SNIGHTQAAAGVASVIKMVEAMRHGVVPRTLHVDEPTPHVDWTAGAVELVTEERPWPET
                     GRARRAGVSSFGVSGTNAHVIVEQAP"
     aSModule        6414..9638
                     /domains="nrpspksdomains_NBY68_RS35205_PKS_KS.1"
                     /domains="nrpspksdomains_NBY68_RS35205_PKS_AT.1"
                     /domains="nrpspksdomains_NBY68_RS35205_PKS_DH.1"
                     /incomplete
                     /locus_tags="NBY68_RS35205"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       6840..6866
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35205_0001"
                     /evalue="2.60E+01"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="186"
                     /protein_start="177"
                     /score="-1.1"
                     /tool="antismash"
                     /translation="GRADSVISG"
     CDS_motif       6894..6941
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35205_0002"
                     /evalue="6.60E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="211"
                     /protein_start="195"
                     /score="21.1"
                     /tool="antismash"
                     /translation="GPALTVDTACSSSLVA"
     CDS_motif       7317..7361
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35205_0003"
                     /evalue="1.50E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="351"
                     /protein_start="336"
                     /score="13.8"
                     /tool="antismash"
                     /translation="VEAHGTGTKLGDPIE"
     CDS_motif       7422..7466
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35205_0004"
                     /evalue="1.50E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="386"
                     /protein_start="371"
                     /score="11.2"
                     /tool="antismash"
                     /translation="GSLKSNIGHTQAAAG"
     CDS_motif       8022..8078
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35205_0005"
                     /evalue="1.80E-09"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="590"
                     /protein_start="571"
                     /score="29.2"
                     /tool="antismash"
                     /translation="VFVFPGQGSQWAGMAVGLL"
     aSDomain        8028..8918
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35205_PKS_AT.1"
                     /evalue="1.40E-104"
                     /label="NBY68_RS35205_PKS_AT.1"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="870"
                     /protein_start="573"
                     /score="341.6"
                     /specificity="substrate consensus: mmal"
                     /specificity="PKS signature: Methylmalonyl-CoA"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="VFPGQGSQWAGMAVGLLDSSPVFAERFGECATALAPYVDWVPVDV
                     LREREGAPSLDRVDVVQPMLWAVMVSLAAVWRASGVRPAAVVGHSQGELAAAVVAGVLS
                     VEDGARIVALRSQLIGRELAGQGGMVSVPLAEGAAVELIESWAGRISVATVNGPRSTVV
                     AGEAAALDELVAVCERDGVRARRIPVDYGSHTPQVERLREALLELAAPVAPRPGDVPMY
                     STVTGALLEDGVADAEYWFRNLRQPVRFLDTVEELIASGHTTFVEVSPHPVLTSSVEET
                     GEVGGVEVCGVGTLRR"
     CDS_motif       8190..8261
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35205_0006"
                     /evalue="8.90E-10"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="651"
                     /protein_start="627"
                     /score="30.7"
                     /tool="antismash"
                     /translation="LDRVDVVQPMLWAVMVSLAAVWRA"
     CDS_motif       8265..8384
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35205_0007"
                     /evalue="1.00E-17"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="692"
                     /protein_start="652"
                     /score="56.2"
                     /tool="antismash"
                     /translation="GVRPAAVVGHSQGELAAAVVAGVLSVEDGARIVALRSQLI"
     CDS_motif       8484..8522
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35205_0008"
                     /evalue="1.10E-03"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="738"
                     /protein_start="725"
                     /score="12.3"
                     /tool="antismash"
                     /translation="ATVNGPRSTVVAG"
     CDS_motif       8592..8624
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35205_0009"
                     /evalue="3.50E-03"
                     /label="PKSI-AT-mM_m6"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="772"
                     /protein_start="761"
                     /score="10.4"
                     /tool="antismash"
                     /translation="VDYGSHTPQVE"
     CDS_motif       8733..8777
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35205_0010"
                     /evalue="7.60E-06"
                     /label="PKSI-AT-mM_m8"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="823"
                     /protein_start="808"
                     /score="17.8"
                     /tool="antismash"
                     /translation="DAEYWFRNLRQPVRF"
     CDS_motif       8820..8849
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35205_0011"
                     /evalue="2.10E-03"
                     /label="PKSI-AT-mM_m9"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="847"
                     /protein_start="837"
                     /score="10.7"
                     /tool="antismash"
                     /translation="FVEVSPHPVL"
     CDS_motif       8997..9032
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35205_0012"
                     /evalue="5.70E+01"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="908"
                     /protein_start="896"
                     /score="-2.4"
                     /tool="antismash"
                     /translation="VFAGSGAGRVGL"
     aSDomain        9123..9638
                     /ASF="catalytic triad H,G,P inconclusive"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS35205_PKS_DH.1"
                     /evalue="6.80E-59"
                     /label="NBY68_RS35205_PKS_DH.1"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="1110"
                     /protein_start="938"
                     /score="190.9"
                     /tool="antismash"
                     /translation="HPLLAAGITIADGEGHLFTGRISLRTHSWLAGHAVLGRVLVPGAA
                     VLELVLRAGESVGCELLDELVLQAPLVVPAGDAAVDLQVAVDAPDEQGRRAVRLHSRAV
                     RPGQEDDGAEWVCHAEGLLTAVPAASAGEQAAADATWPPAGAEAVDIDGFYDRLAATGY
                     GYGPIFQGI"
     CDS_motif       9207..9281
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35205_0013"
                     /evalue="8.80E-08"
                     /label="PKSI-DH_m2"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="991"
                     /protein_start="966"
                     /score="24.7"
                     /tool="antismash"
                     /translation="WLAGHAVLGRVLVPGAAVLELVLRA"
     CDS_motif       9438..9491
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35205_0014"
                     /evalue="3.60E+01"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="1061"
                     /protein_start="1043"
                     /score="-1.8"
                     /tool="antismash"
                     /translation="PGQEDDGAEWVCHAEGLL"
     CDS_motif       9918..9959
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS35205_0015"
                     /evalue="3.70E+01"
                     /label="PKSI-KS_m3"
                     /locus_tag="NBY68_RS35205"
                     /protein_end="1217"
                     /protein_start="1203"
                     /score="-1.7"
                     /tool="antismash"
                     /translation="PVLTIDTLTSRPLT"
ORIGIN
        1 gccgcccacg tcggtgtgcg gtaccgggcc ttcgacctgg gtgaggcggg cgccgaccgg
       61 ctcggtgaga tgctcgcgca cctggcggaa ctcttcgccc ggggggagct ggtgccgctg
      121 cccgtgaccg cgtgggacac ccgccaggcg ccgcaggctt tccggcacat gagccaggcc
      181 cggcacatcg gcaaggtcgt gctgaccaac ccgcgtgccg ctctggacgg caccgtcctg
      241 gtgaccggtg ggaccggcgt catcgggtcc gccgtggccc gccacctggt cactgggcac
      301 ggcgtgcggg acctggtgct caccggccgc cgtggccagg acgccccggg ggcccgggag
      361 ctcgcggccg aactgaccgg gctcggcgcc accgtgcgcg tcgaggcctg tgacgccgcc
      421 gaccgtgcgg cccttggcac gctcctggac gggctcgacg ggctgtgcgg tgtggtgcat
      481 gctgccggag ccctggacga cggcgtcctc tccgcgctca cccccgagcg tctggagacc
      541 gtccttcgcc cgaagctgga cgcggcctgg aacctgcacg agctgacccg cggccgtgac
      601 ctcgcgttgt tcgtgctctt ctcctcggcc gcgggtgtct tcggctctcc ggggcagggt
      661 ggctacgcgg cggcgaattc cttcctcgac gcccttgccc aggagcggcg tacccagggg
      721 ttgcccgcgc actccctggc ctgggggctg tgggccgacc gcagtgccat gaccggcgcg
      781 ctcagtacca ctgacctcga ccgcatgcgg cgcgtgggtg tccggccgtt ggccaccgag
      841 gagggcctcg ccctcttcga cgccgcggtg cgcctccccg acccgcacac cgtcccggtc
      901 cgcctcgaca cggccacgct gcgcgaacag cggcccctgg ccccgctgtt ccgcgccctg
      961 gtgcgcacca cggccaagcg ggccgccgcc accacccccg cgggcagcgg cgacgggctg
     1021 cgcgagcgcc tcgccgcact ggcccccacc gagcgcgacc gcgagctgac ggagctggtg
     1081 cgcgcccagg ccgccctcgt cctcggccac ggcaccgccg gcgcggtgga ggccggccgg
     1141 gccttccgcg acatgggctt cgactcgctg accgccgttg aactgcgcaa ccggatcggc
     1201 tctgccaccg gtctgcgcct gaccgtgacc gctgtcttcg accaccccac ccccgccgcc
     1261 ctcgcggccg aggtcgccat ccggctcggc atcgacggcg acatcggccg ggagcgcacc
     1321 acccccaccg ccgcaccggc catcgccgcc gacgacgacc cgatcgtcat catcggtatg
     1381 agctgccgct tccccggcca catcgactcg cccgaaacac tgtggcaact gctcaccgac
     1441 gaacgcgacg ccatgggcga ctaccccacc gaccgcggct gggactccgt cgaccttcac
     1501 aaccccgagg cccccgacct gcacttcgcc aaggtcggcg gattcctgca cgacatggcg
     1561 gacttcgacc ccggcttctt cggaatgtcg ccgcgcgagg cgcaagccac cgacccgcag
     1621 cagcggctgc tcctcgaggc cacctgggag gcgctggagc gggccggcat cgacccggcc
     1681 accctgcgct ccaccccgac cggcatcttc accggcctga tctacaacga ctacgccgcg
     1741 cgcttccccg acctgctcag cggatacgag ggctacctcg gcaacggcag cgccaacagc
     1801 gtcgcctccg gccgcatcgc ctacacgttc ggcctggagg gcccagcgat cacggtcgac
     1861 acggcgtgct cctcctcgct ggtcgccctg caccaagccg cgcacgccct ccgcaacggc
     1921 gactgcacac tcgcggtcgc cggcggcgtc accgtcatgt cgaccccgcg cccgatggtg
     1981 gagttcagcc gcatcagcgg tctctcgccg gacggccgct gcaaggcctt ctccgccgag
     2041 gccaacggca tgggcttcgc cgagggcgtc ggcatgctcg tcgtggagcg gttgtcggac
     2101 gcgcgccgca acggccaccc cgtcctcgcg gtcctccgtg gctccgccct caaccaggac
     2161 ggcgcctcca acggcctgac cgcgccgagc ggccccgcgc aacagcgcgt catccgccag
     2221 gcgctcgcca acgccgggct caccaccgcc gacgtcgacg tcgtcgaggc acacggcacc
     2281 ggaacgtcgc tcggcgaccc catcgaggcc caggcggtcc ttgccacgta cggcaaggac
     2341 cgccccgagg acaagccgct gctgctcggc tcggtgaagt ccaacatcgg tcacacccag
     2401 gccgcggccg gtgtcgcggg cgtcatcaag atggtgctcg ccctgcgcca cggtgtggtg
     2461 ccgaggacgc tgcacgtgga cgagccgacg ccgcacatcg agtgggactc cgccggtgtg
     2521 cggctcgccg acgcgagtgt gccgtggccc gagacgggca ggccgcggcg cggcgccgtc
     2581 tcctcgttcg gcatcagtgg caccaacgca cacgtcatcc tcgaacacgc tcccgagccc
     2641 gagcccgagc ccgagcccga gcccgagccc gaggccgtcg aggacacccg tgaccacctg
     2701 ccgtgggtga tctccgccaa gaacccggag agcctgcgca agcaggcact gcgcctgcac
     2761 gcccacgtca gcgccgcccc cgaggccacc ctcgtcgaca tcgcacgctc gctcctgacc
     2821 acccgggcag gattcgagca ccgcgccgtc gtcatcggcc gcgaccgcga ggaactgctg
     2881 cggcgcctgg ccgcgttcgg ccacggcgac gagacccccg gcgtgatcca gggcaccgcg
     2941 gcaagcgaca ccaggaccgc cttcgtcttc cccggccagg gctcgcagtg ggcgggcatg
     3001 gcgaccgaac tcctggactc ctcactggcg ttcgcccgtc ggttcggcga gtgtgcggcg
     3061 gccctcgccc cccacatcga ctgggagccc gcagacgtgc tgcgcgagcg cgaggacgct
     3121 ccgtcgctgg agcgcgtgga cgtgatccag ccgatgctgt gggccgtcat ggtctcgctc
     3181 gccgcggtgt ggcgcgcgca cggcgtcaag cccgctgccg tagcggggca ctcccagggt
     3241 gagttggcgg ccgccgtggt cgccggcgtg ctctcactcc aggatgccgc gcgaatcgtc
     3301 gcgctgcgca gccagctgat cgggcgtgaa ctggcaggtc agggtggcat ggtctccgtg
     3361 ccggtcggcg aggacgcggc ggaggagctg atcgagccct gggcgggccg gataagcgtc
     3421 gccacgatca acggcccgca gtccacggtc gtcgccggtg aggggccggc gctggacgag
     3481 ctgatggccg cgtgcgagcg cgacggtgta cgggcccgtc gcatcccggt ggactacggc
     3541 tcccacaccc cgcaggtcga gcagctccgc gaggccctcc ttgagctcgc cgctcccgtg
     3601 accccgcgcc cggcccaggt gccgatgtac tcgaccgtca ccgccgagcc actcgaggac
     3661 ggtatggccg acgccgagta ctggtaccgc aacctccgcg aacccgtccg cttccagcag
     3721 accgcgcggg ccctcgccga cgcgggccac ggtgtcttcg tcgaggtcag cccgcatccg
     3781 gtcctgacca ccggcatcga ggccagcctc gcggacctcg atgccgcccc cgtggtcacg
     3841 ggcaccctgc ggcgcgacga cggcggccgc agcagactcc tgaccgcact cgccgaactg
     3901 tacgtcgccg gcgcccccgt ggactggtcg agcaccttcg acggtggcag ggccgtggac
     3961 ctgcccactt atgccttcca ccggcagcgc tactggctgg acgcccccgc gcccgcgcag
     4021 ggcaccgcgg actccggcgt cgacagcgcc ttctggacgg cggtcgacgg cggcgacacc
     4081 gaatcgctct ccgccgcgct cggcgtggcg gacgactccg tgcgctcctc gctcgccacc
     4141 gtcctgcccg cgctctccgc ctggcacggc aagcagcgcg aggcttccgc cgtggacgcc
     4201 tggcgctacg ggatcgaatg gacgccggtc caggacagcc aggcccgcct cacgggcacc
     4261 tggctgctcg ccgtgcccga ggagcgcgcc gacgacccgg cggtacggga aatcggcgcg
     4321 ggcatcgagc gccgcggcgg ctccgtccgc caggtcctgc tccccgcgga gcgcgccgag
     4381 gacgtcgcca ccacgggcga actcccgtac gtcgccgaga tctccgtacg actgcgcgcc
     4441 gtcgaaggac acgagcgggc ggccggagtc ctcgcgctga ccggacggga cgaggactcc
     4501 cacccggcgc accccggcgt gcccctcggc ttcacccgca cccttgggct gatccaggct
     4561 ctggccacca ccgcggtcac cgcccccatg tggtgcgtca ccagcggcgc ggtcaccacc
     4621 ggcagcagcg ccgaactcac cgcgcccagc caggcgatgg tgtggggact cggccgggtc
     4681 gccgcccagg agtacccgga ccgctggggc ggcctgatcg acctgcccgc cgagccgggg
     4741 gagcgggacg tcgaccggct ctgcgccctg ctgaccggcg agaccggcga ggaccagctc
     4801 gcggtgcgcc cctccggcgt ccgcgcgagg aggctggtgc gcaccgccgc gtccgcggga
     4861 cgggacggcg cctggcagcc ccccaagggc accatcctga tcaccggcgg taccggcgcg
     4921 ctcggcggcc acatcgcacg ctggctcgcc gaacggggcg ctgagcacct gatgctcctg
     4981 agcaggagcg gccccgatgc cgagggcgcc gacgagctgc gggcccaact gtccgctgcc
     5041 ggagcgcggg tgaccgtcct gtcctgcgac gcgggcgacc gcgacgcatt ggcagccgcc
     5101 ctggccggca tcccggagaa gctgccgctg accgcggtct tccacaccgc cgccgccctg
     5161 gacgacggcc cgctggagac gctgaccctc ccccgcgtag ccgcctcgct ccgctcgaag
     5221 gcgggcgctg cgtggaacct ccacgagctg accgcggacg cggacctgtc ggcgttcgtg
     5281 ctcttctcct ccacggcggg caccttcggc gccgcgggcc agggcaacta cgcgccgggc
     5341 aacgcctact tggacgcgct ggcctggtac cgcagggccc acggccaggt ggcggcctcc
     5401 atcggctggg gcgcctgggc ccagggcggc ttggcggaga aggacgccgt cgctgacctg
     5461 cgccgcaggc acggcgtacc gctcatgtcc ccgcagcgcg ccaccctcgc catggagcgg
     5521 gccctggcag gcgacgacac cttcgtggtc cttgccgaca tcgagtggga caaattccag
     5581 ctcgcttaca ccgccgcgcg gcccagtcct ctgctgtacg gcctcgcgga ggtccagcgc
     5641 ccccagaagg ctgtggacgg ggcggatacc gccggcacgg atggtcctag ttttgccgaa
     5701 cgtctgttcg gcatggctcg caaggagcag gtccggatgc tgcgcgaggc ggtccgcacc
     5761 cacgccgcgg ccgtcctcgg ccatgacagg gccgaatcgg tgccgctcac ccgcccgttc
     5821 gcggagctcg gcctggactc ggtcaccgcg gtggagctgc gcaaccgcct cggtggcgtg
     5881 gtcggccaca agctgccggc cgcgctcgtc ttcgaccatc cgacggtcgc cgacctcgtt
     5941 gcctatctgc acgacctgtt gttcggcgac gagacgtaca gcagcccgat gctggccgaa
     6001 ctggaccgcc tggatgttga gctggccacc ctgcgtgacg acgacccggt caggggcgag
     6061 gtggcggagc ggctggagaa actgctgcgc cgtgtcagcc ccgactcggt cgtctcctcg
     6121 ggcggcaggg ccgcgcagag ctgggaccta caggctgcct cccatgacga agtgttcgca
     6181 atgatcgatg acgagctggg gaagccgtga tccggaccgg ccggacgctc tgagcgccgc
     6241 ccttgtgaat ccagccggaa cacgactgcc gaccttatta gtgctgctaa ggaatggacc
     6301 cgccgcccat gccgaatgaa gagaagctct tcgactacct caagcgcgtg accgccgagc
     6361 tgaaggagac caagtcacgg ctgaaagcgg tggagtcggc cggtagcgag ccggtggcga
     6421 tcgtcgggat gagctgccgc taccccggcg gtgtcgcctc gcccgaagat ctgtggcggt
     6481 tgctttccga gggcggcgac gccatcacgg gcctgcccgc cgaccgcggc tgggatcctt
     6541 cgtcgctgat cgacgtgacc ggcgagcgcc ccggctcctc gtatgtggcc gaaggtggat
     6601 tcctccacga cgcgggggag ttcgacgccg gcttcttcgg gatatccccg cgcgaggccc
     6661 tggccatgga cccgcagcag cggctgctcc tggagacctc ctgggagctg ttcgagcgct
     6721 ccggcatcaa ccccaccacg ctgcgtggca gcgacaccgg tgtcttcgtg ggctccagct
     6781 tccacgggta cggcgacccg gccaccgccc ccgacgaggc gcagggttat ctgctcaccg
     6841 gccgcgccga cagcgtcatc tccggccgga ttgcatacac cctgggcctc gaaggcccgg
     6901 cactcactgt cgacacggcg tgctcgtcgt cgctggtggc gttgcacctg gcgatcaatg
     6961 cgctgcagct cgaggagtgt tcgttggcgc tcgcgggcgg cgtgatggtc atgtcgacgc
     7021 ccgaagtctt cgtggggctc agccggcagc agggtctggc gcgggatggt cgctgcaagg
     7081 cgttcgccgc ctctgccgat ggcatgagcg cgtcggaggg tgtgggtctg ctgttggtgg
     7141 agcggctctc ggacgcgcgc cgcaacggcc accaggtcct tgccgtgatc cgtggttcgg
     7201 cggtcaacca ggacggtgcg tccaacggtc tgaccgcgcc ccatggcccc gcgcagcagc
     7261 gggtgatccg gcaggctctg gagagcgccg ggcttgttcc cgcgcaggtg gacgtggtcg
     7321 aggcgcatgg tacgggtacc aagctgggcg acccgatcga ggcgcaggcg ctgctcgcca
     7381 cgtacggcca ggaacgggac gaggagcagc cgctgctgct cggctcgttg aagtccaata
     7441 tcggtcacac gcaggctgcc gcgggtgtgg cgagtgttat caagatggtc gaggcgatgc
     7501 ggcatggtgt ggtgcccagg acgctgcacg tcgatgagcc gacgccgcat gtggactgga
     7561 ccgcgggtgc cgtcgagctg gtgaccgagg agcgtccgtg gcccgagacg ggtcgcgcac
     7621 gccgtgcggg cgtgtcgtcg ttcggtgtga gcgggacgaa cgcgcacgtc atcgtcgagc
     7681 aggccccggc cgaggagccc cccgaagccg aagccgaagc cgaggccggt ccggcgggcc
     7741 ttgtgtcggg cggtggtgtc gtgccgtggg tgctctcggg ccggtcggca gccgggctgc
     7801 gcggacaggc ggagcggctg caccggttcg tggcctcctc cgaggcctcg atcgccgacc
     7861 tgggctggtc gctggcatcc tcgcgtgcgg gactcgggca ccgggccgtg ctgctcggcg
     7921 cggaccagga ggaactggtg accgccctga aggccctgga ggaaggtgaa ccggcggctg
     7981 gtgtggtcac gggtgtggcc tccgaaggcg cttcggacgt cgtgttcgtc ttcccgggtc
     8041 agggttcgca gtgggctggg atggcggtcg ggctcctgga ttcctctccg gtgttcgcgg
     8101 agcggttcgg tgagtgtgcg actgctctgg cgccgtacgt cgactgggtt ccggtcgatg
     8161 tcctgcggga gcgcgagggt gcgccgtcgt tggatcgtgt ggatgtggtc cagccgatgc
     8221 tgtgggcggt catggtgtcc ctcgccgcgg tgtggcgtgc gtccggcgtg cggcccgccg
     8281 ccgttgtcgg ccattcgcag ggtgagttgg cggctgccgt ggtggcgggt gtgctgtcgg
     8341 tcgaggacgg tgcgcggatc gtggcgctgc gcagtcagtt gatcgggcgg gagctggccg
     8401 gtcagggtgg catggtgtcg gtgccgctcg cggagggtgc ggcggtggag ctgatcgagt
     8461 cctgggcggg ccggatcagt gtggccacgg tcaacggccc gcggtccacg gtggtcgcgg
     8521 gtgaggcggc ggcactggat gagctggtgg cagtgtgcga gcgcgatggt gtgcgtgcgc
     8581 gtcgtattcc ggtggattac gggtcgcata ctccgcaggt ggagcgattg cgggaggctt
     8641 tgctggagct ggctgctccg gtggcgccgc gtccgggtga tgtgcccatg tactcgaccg
     8701 tgactggtgc gctcctcgag gacggtgtgg ctgatgctga gtactggttc cgcaatctgc
     8761 ggcagccggt gcggttcctt gacacggtgg aggagctgat tgcttcgggc cacaccactt
     8821 tcgtcgaggt cagtcctcat cccgtgctga cgtcttcggt ggaggagacc ggtgaggtgg
     8881 gaggtgtgga ggtctgcggg gtcggcacgt tgcgacgtga tcagggtggt gtccggcgtt
     8941 tcctcacttc gctggcggag ctttgggtgc ggggtgtgga gccggagtgg cagggtgtgt
     9001 tcgccggcag tggtgcaggc cgggtcgggc tgcccacgta tgccttccag cgccggcact
     9061 actggctgga cggtgcggcc ggaacggctg aggtgtccgg agccggactc tccgcgctcg
     9121 gtcacccgct gctggctgcc ggtatcacga tcgcggacgg cgagggccac ctcttcacgg
     9181 gccgaatatc cctgcgtacg cattcatggc tcgccggtca cgcggtgctg ggccgggtcc
     9241 tggtgcccgg ggccgccgtg ctcgaactgg tgctgcgggc gggcgagagc gttggctgtg
     9301 aactcctcga cgagctcgtt ctgcaggccc ctctggtcgt accggccggc gacgcggcgg
     9361 tcgacctcca ggtcgccgtc gacgcccctg acgaacaggg ccggcgcgcg gtgcggttgc
     9421 actcccgggc ggtgcggccg ggccaggagg acgatggtgc ggagtgggtc tgccacgccg
     9481 aggggctgct gaccgcggtg cccgccgcat ccgccggtga gcaggccgcg gccgacgcca
     9541 cctggccgcc cgcgggcgcg gaggccgtgg acatcgacgg tttctacgac cgcctggcgg
     9601 ccaccgggta cggctacggt ccgatcttcc agggcatacg ggcggtatgg cggcgcggcg
     9661 aggaactgtt cgccgatgtc gtactgccgg agggtggcga gcgggatgcc gggcggttcg
     9721 gggtgcaccc cgcgctggtc gacgccgcgc tgcagacggg tctggtgacg ttgctggagg
     9781 acggcggcga gaggatgatg ccgttctcct ttgccggggt tcgcctccac gcgaccggtg
     9841 ccaccgcggc acgggtccgg ctgacgccga cgggcgctga ctcgctcgcg gtgcggctga
     9901 ccgacggcac cggccttccg gtactcacca tcgacacgct cacctcgcgc ccgctcaccg
     9961 ctgacacgct ctccggccag agcgtggact cgttgtatga ggtcaactgg gcgctgttta
    10021 ccggcggttc gggtgacatc accagtcttc ccggctcggc agtggttctg ggtgaaggtc
    10081 tggcggagct cggtctgccg cgtcatgagg acctcgccgc gctcgccgag agcgtccgcg
    10141 gcggcggcgc cgtgcccgcg accgtgctcc tgccctgccc gcggacggcg gccggagaca
    10201 cccccgccgc cgtccgcgcg ctgctggcct ccgtgctggg cac
//
