LOCUS       NZ_CAKMXI010000003     10927 bp    DNA     linear   CON 03-JUN-2024
DEFINITION  Streptomyces atroolivaceus strain TUE2108, whole genome shotgun
            sequence.
ACCESSION   NZ_CAKMXI010000003
VERSION     NZ_CAKMXI010000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces atroolivaceus
  ORGANISM  Streptomyces atroolivaceus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 673445)
  AUTHORS   Wibberg,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2022) CEBITEC, Genome Research of Industrial
            Microorganisms (GRIM), Universitaetsstrasse 27, 33615, NRW, Germany
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_928722305.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 04:23:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,057
            CDSs (total)                      :: 6,983
            Genes (coding)                    :: 6,683
            CDSs (with protein)               :: 6,683
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 300
            CDSs (without protein)            :: 300
            Pseudo Genes (ambiguous residues) :: 0 of 300
            Pseudo Genes (frameshifted)       :: 123 of 300
            Pseudo Genes (incomplete)         :: 254 of 300
            Pseudo Genes (internal stop)      :: 20 of 300
            Pseudo Genes (multiple problems)  :: 87 of 300
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:59:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 403387
            Orig. end                         :: 414314
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CAKMXI010000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10927
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10927
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10927
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5927](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5927)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(434..449)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.54"
                     /tool="antismash"
     misc_feature    542..557
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.57"
                     /tool="antismash"
     misc_feature    complement(896..911)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.11"
                     /tool="antismash"
     gene            1361..1672
                     /locus_tag="NBY68_RS09510"
     CDS             1361..1672
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092371.1"
                     /locus_tag="NBY68_RS09510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SCO5918 family protein"
                     /protein_id="WP_250288422.1"
                     /transl_table=11
                     /translation="MRCVIARFPFDLFKNEVEESMKGIKPEPVTGDAVVISRRVYPVKQ
                     VGEIITRQDRRDFTAGEVTRALTRLGFTCRPAEVPAPAAPPLTPLENASALLGTPSDA"
     gene            complement(1736..2812)
                     /locus_tag="NBY68_RS09515"
     CDS             complement(1736..2812)
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155343.1"
                     /locus_tag="NBY68_RS09515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyltransferase family protein"
                     /protein_id="WP_250288423.1"
                     /transl_table=11
                     /translation="MPSLTSRPPGPAAAGAGAPATAPSRPGRDPFFDNAKYLAIVLVAM
                     GHSWEPLPGSGRVVETFYTFVYTFHMPVFIIIAGYFSRGLELRSDRVWKLVTSLAVPYA
                     VFEIAYTLFERLTGTPTFPLSPLTPYWLLWFLPALFLWRLTTPFWQTVRLPVAVSLVIA
                     AMGAVTSNIGGDLHLQRILQFLPYYVLGLQLRPEHFDFVRSRAMRIASVPVCLVALAAA
                     YWAVPRTTTSWFFRRYGAEQLGSPAWAGAPVTLLLFACALLLSACFLAWVPNRRLWMTA
                     LGAGTLYGYVLHGFVIKGAIKLDLFEVDIVSTPLGRLLVTAAAAVMVTALCSPPVQRVF
                     RYAVEPRLDWARKPRPAP"
     gene            complement(3048..3977)
                     /locus_tag="NBY68_RS09520"
     CDS             complement(3048..3977)
                     /NRPS_PKS="Domain: PKS_ER (20-300). E-value: 1.6e-37.
                     Score: 121.2. Matches aSDomain:
                     nrpspksdomains_NBY68_RS09520_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155344.1"
                     /locus_tag="NBY68_RS09520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="zinc-binding dehydrogenase"
                     /protein_id="WP_250288424.1"
                     /sec_met_domain="ADH_zinc_N (E-value: 5e-19, bitscore:
                     63.8, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLAIQFDRFGGPEVLRPVRMAPPEPGPGEVLLTVEAAGVNFADTH
                     QVDGSYLGADALPHVPGSEVVGRTPDGRRVLARVSHGYAEQVTAKESGVLEIPEELGAA
                     QALALMVQGLSAWHLLRSAARLRTGESVVVHSAAGGVGSLAVQLAREFGAGRVLAQVST
                     PAKERLVLDLGADAVITYPLPEKADVVLDAVGGHLFDQALDSLASFGRLVTYGNAARTG
                     FTPVDPGRLGRLNASVVGFWLRPALAAPGAVGGPLRELFALVEAGRLKPVTGGSYPLAD
                     ARRAHEDLLARRTTGKLVLTPPPDPLIP"
     aSDomain        complement(3078..3917)
                     /ASF="ER configuration inconclusive"
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS09520_PKS_ER.1"
                     /evalue="1.60E-37"
                     /label="NBY68_RS09520_PKS_ER.1"
                     /locus_tag="NBY68_RS09520"
                     /protein_end="300"
                     /protein_start="20"
                     /score="121.2"
                     /tool="antismash"
                     /translation="APPEPGPGEVLLTVEAAGVNFADTHQVDGSYLGADALPHVPGSEV
                     VGRTPDGRRVLARVSHGYAEQVTAKESGVLEIPEELGAAQALALMVQGLSAWHLLRSAA
                     RLRTGESVVVHSAAGGVGSLAVQLAREFGAGRVLAQVSTPAKERLVLDLGADAVITYPL
                     PEKADVVLDAVGGHLFDQALDSLASFGRLVTYGNAARTGFTPVDPGRLGRLNASVVGFW
                     LRPALAAPGAVGGPLRELFALVEAGRLKPVTGGSYPLADARRAHEDLLARRTTGKLVL"
     CDS_motif       complement(3531..3593)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS09520_0001"
                     /evalue="1.50E-07"
                     /label="PKSI-ER_m5"
                     /locus_tag="NBY68_RS09520"
                     /protein_end="149"
                     /protein_start="128"
                     /score="23.9"
                     /tool="antismash"
                     /translation="GESVVVHSAAGGVGSLAVQLA"
     gene            4232..4996
                     /locus_tag="NBY68_RS09525"
     CDS             4232..4996
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (8-171). E-value: 2.1e-15. Score:
                     49.1. Matches aSDomain:
                     nrpspksdomains_NBY68_RS09525_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155345.1"
                     /locus_tag="NBY68_RS09525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_250288425.1"
                     /sec_met_domain="adh_short (E-value: 5e-35, bitscore:
                     116.3, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 1.3e-32, bitscore:
                     108.9, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDLLHDRVVLVTGASSGIGAAAARVFAEEGATVVLAARREDRLA
                     SLVAELKDKGAQASYVVCDVTVASDAARAVGFTVDTYGRLDSAFNNAGLGGDRTPLHLM
                     GDDVYDAVMDVNVRGVWNCLRHEIAAMLQNPGNCSIVNNSSVGGLVAIPAAAPYIAAKH
                     AVVGLTRAAADEYAQQGIRVNAVAPGTTRSEITADWFGRNPGLEEMVNSLTPQGRTAEP
                     EEIANAAAWLLSDRCPFLTGTVMPVDGGFVNQ"
     CDS_motif       4256..4351
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NBY68_RS09525_0001"
                     /evalue="9.70E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="NBY68_RS09525"
                     /protein_end="40"
                     /protein_start="8"
                     /score="15.0"
                     /tool="antismash"
                     /translation="VVLVTGASSGIGAAAARVFAEEGATVVLAARR"
     aSDomain        4256..4744
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NBY68_RS09525_PKS_KR.1"
                     /evalue="2.10E-15"
                     /label="NBY68_RS09525_PKS_KR.1"
                     /locus_tag="NBY68_RS09525"
                     /protein_end="171"
                     /protein_start="8"
                     /score="49.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VVLVTGASSGIGAAAARVFAEEGATVVLAARREDRLASLVAELKD
                     KGAQASYVVCDVTVASDAARAVGFTVDTYGRLDSAFNNAGLGGDRTPLHLMGDDVYDAV
                     MDVNVRGVWNCLRHEIAAMLQNPGNCSIVNNSSVGGLVAIPAAAPYIAAKHAVVGLTRA
                     "
     gene            complement(5001..5927)
                     /locus_tag="NBY68_RS09530"
     CDS             complement(5001..5927)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="NBY68_RS09530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_284347571.1"
                     /sec_met_domain="AfsA (E-value: 1.2e-17, bitscore: 58.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGRAVPDIPAVPCPPLSAAEGRDGIRKEYVHLGRTDMVLLTSWR
                     RREDDEFSLSARWPAPPAGLPYDPRMLTQTVRQSGLTIAHAAYGAPLSDHTLLNYFDFT
                     VTPGFEVPSEGACDLTVEVTVRNAKERGRKVSSLDMEFRILRGGSVAVRADSEFGWLST
                     RVYRRLRGEHFTVDWNAWPLPAPVDPRTVGRSTPADVVLAAGDRPRHWQLRNDVDNAVL
                     YDHPVDHVPGLALMEAADQAAHAAVHPARFEATTVTSTFERYAEFDRPCWVEAEILPAA
                     PGETSVLVTATQDGETVFRSRLSGPCG"
     misc_feature    5974..5983
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.37"
                     /tool="antismash"
     gene            6019..6663
                     /locus_tag="NBY68_RS09535"
     CDS             6019..6663
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155347.1"
                     /locus_tag="NBY68_RS09535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_033296651.1"
                     /transl_table=11
                     /translation="MARQQRAIRTRRIFLQAAAEVFDEHGYDAATIAAILDRAGLTRGA
                     LYFHFTSKEELARGVLQEAVTSDGVTPQTFKLQEWVDMALLLAYRLPREPLLSASIRLS
                     VDPRARGLFGTRWPDWIALGTEMLTEAKERGELLPHVDPEATSRLVVGAWTGVQLVTES
                     MAEPPDLVAEISALFELILPNNAVPGVLARLDTSPHRAARIMEQQRAVAAS"
     gene            6757..7491
                     /locus_tag="NBY68_RS09540"
     CDS             6757..7491
                     /EC_number="3.5.1.28"
                     /GO_function="GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA]"
                     /GO_process="GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155348.1"
                     /locus_tag="NBY68_RS09540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="WP_033296650.1"
                     /transl_table=11
                     /translation="MWLRRTAVCATVVPLAFLVFRDAPVRLAAPEQATAKPATPAGAQR
                     PAIVTRAQWHADESLVKEDAVYTGVVKAVFVHHTGHTNAYDCADAPEMLRAMEEAHVRG
                     EGWDDIGYNFVVDRCGTIYEGRAGGVGRSVRGAHATGFNAHSVGIAALGTFDPGVPVPK
                     ALIEGIAKVGAWKLDPAVDPGGIVRMVSTNDESLYRRGEVVYLHAVAGHRDTFQTYCPG
                     EALYAQLPAVRAEMARLRALKG"
     gene            complement(7495..7860)
                     /locus_tag="NBY68_RS09545"
     CDS             complement(7495..7860)
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514034.1"
                     /locus_tag="NBY68_RS09545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PH domain-containing protein"
                     /protein_id="WP_250288426.1"
                     /transl_table=11
                     /translation="MALFGNAHSVDPAAAQRDYARLLGHGEQVHAAYLLIRDTILFTDR
                     RLVLIDKQGMTGKKVEYHSVPYRSITHFAVETAGTFDLDAELKIWVSGSPAPIQKTFTK
                     GVDIYEVQAVLTQYVAR"
     misc_feature    7915..7926
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.86"
                     /tool="antismash"
     gene            7986..8828
                     /locus_tag="NBY68_RS35335"
     CDS             7986..8828
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047018.1"
                     /locus_tag="NBY68_RS35335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transglycosylase family protein"
                     /protein_id="WP_284347572.1"
                     /transl_table=11
                     /translation="MPASPLAKRPVESVLALLLVLLSVLGLSGRSAADAPAQAPAQAVP
                     AQAVAAQAVPAQAVPARAVIAPTVTAGTDWDRIAECESNGRWNINTGNGYHGGLQFAPS
                     TWRAYGGGQYAPRADLATRSEQIAVGERVARSQGMGAWPTCGRLGANGTSNSSSSSSSS
                     SSSSSDGADRSDASQAAPQRQSAGSSNSGSSGASSSAGSQAGSTSGSSPRGDSGVTHHS
                     EDLADAETYVVESGDCLSVIAARADVSGGWPALYELNREVLDQGPHLIFPGQRLRLNA"
     gene            complement(9545..9769)
                     /locus_tag="NBY68_RS09560"
     CDS             complement(9545..9769)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015609659.1"
                     /locus_tag="NBY68_RS09560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_033296647.1"
                     /transl_table=11
                     /translation="MARTERQTDRQAETEYVCPACKQPVATVLARHKTLGIFVPHWGPG
                     PCHNHDCRRHQAESGNAPATARDADSVGR"
     misc_feature    9804..9818
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.99"
                     /tool="antismash"
     gene            9873..10634
                     /locus_tag="NBY68_RS09565"
     CDS             9873..10634
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155352.1"
                     /locus_tag="NBY68_RS09565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HAD-IA family hydrolase"
                     /protein_id="WP_250288427.1"
                     /sec_met_domain="HAD_2 (E-value: 1.3e-11, bitscore: 40.1,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARAPSPAAVLLSVHAVVFDTDGVITDSAPAHAAAWKEAFDACLA
                     LHEGQRPFDEGDDYRRHVDGKSRKDGAAAFLASRGLRIPEGSPDDPPGTATVWAVAAHK
                     DEVFTRGLRTHPIEVWPGTVRLLRVLHDARVPFAAASASRHATELLAGAGLLDLFTAVV
                     DGNEARRLELPGKPDPALFTEAARRLGVPAGETAVVEDALAGVEAGRRGGFGLVVGVDR
                     TDSTEGAAALRRHGADLVVADPGDLLINGED"
ORIGIN
        1 cccctgctcg cggcgctggc ttctgaaggc atgtccgtgc cgttccccat ccagggggcc
       61 acgctgccga actcgctcgc aggacgcgac gtcctgggcc gtggccgcac cggatcgggc
      121 aagacgctcg ccttcggcct ggccctgctc gcccgcaccg cgggcaaccg cgccgacgcg
      181 cgccgtccgc tggccctggt cctcgtcccc acccgggaac tcgcccagca ggtcaccgac
      241 gcgctcaccc cgtacgcccg ctccctcaag ctccggctgg ccacggtcgt cggcgggatg
      301 tcgatcggcc gccaggccag cgcgctgcgc ggcggcgccg aagtcgtggt cgccaccccg
      361 ggccgcctca aggacctcat cgagcgcggc gactgccggc tggaccgggt cgccatcacc
      421 gtcctcgacg aggccgacca aatggccgac atgggcttca tgccccaggt caccgagctg
      481 ctggaccagg tgaacgccga cggtcagcgg atgctgttct cggccactct ggaccgcaac
      541 gtggacctgc tggtccgccg ctacctgcac gacccggtcg tccactcggt ggaccccgcg
      601 gccggagccg tgacgacgat ggagcaccac gtgctctacg tccagggcgc cgacaagtac
      661 gcgacgacca ccgagatcgc cgcacgcgag ggccgggtga tcatgttcct ggacaccaag
      721 cacgcggtgg acaagctcac cgaccacctg ctgcacagcg gcgtgcgggc cgcggcgctg
      781 cacgggggca agtcgcagcc gcagcgcacc cgcaccctcg accggttcaa gaccggtcat
      841 gtcacggtgc tcgtcgccac gaacgtcgcc gcccgcggca tccacgtcga caacctggac
      901 ctggtcgtca acgtcgaccc gcccagcgac cacaaggact acctgcaccg cggcggccgt
      961 accgcgcgcg ccggggagtc cggcagtgtc gtcacgctgg tgctgcccaa ccagcgccgc
     1021 gagatgaccc ggctgatggc cgacgccggg atcatcccgc aggtcgccca ggtccgctcc
     1081 ggcgaggccg agctcaaccg catcaccgga gcgcaggcgc cctcgggcgt gcccgtcacg
     1141 atcacctcac cggtgacgga gcgcaagagc agcggttccg gcccccgtgg ccggcgcggc
     1201 gcgcgtcccg gccaggggcg ccgctccaac gccaacacgg cgacgccgca ggcacgtgcc
     1261 gcggcggcgc agcgccggtc caacaaggcc gcgtagtcac ccggccggga gcccggccgc
     1321 ccctgtccgg ctttcttcgc gaccatcttc ttgaggcacc atgcgctgtg tcatcgcccg
     1381 tttccccttc gacctcttca agaacgaggt ggaggaatcg atgaagggca tcaagcccga
     1441 acctgtcacc ggtgacgcgg tggtcatcag ccgccgcgtc tatcccgtga agcaggtcgg
     1501 cgagatcatc acccggcagg accgccggga cttcaccgca ggcgaggtga ccagggcgct
     1561 gacgcgtctc ggcttcacct gccggccggc ggaggttccc gcacccgcgg cgccgccgct
     1621 cacgccgctg gagaacgcgt ccgccctgct cggaacgccc agcgacgcct gatctgccgc
     1681 tccgtcccgc acccccgagg ccctcccggc ccgggggtgc ggtgcgtccg gggtgtcagg
     1741 gggcgggcct gggcttcctg gcccagtcga gccggggctc caccgcgtac cggaacaccc
     1801 gctggacggg cgggctgcag agggccgtga ccatgaccgc ggcggcggcg gtcacgagga
     1861 gccggcccag cggggtggag acgatgtcca cctcgaagag gtccagcttg atcgcgccct
     1921 tgatgacgaa gccgtgcagt acgtagccgt agagcgtgcc cgcgcccaga gcggtcatcc
     1981 acagcctgcg gttcggcacc caggcgagga aacaggcgct gagcagcaga gcgcaggcga
     2041 acaggagcag ggtcaccggg gcgcccgccc aggcgggcga gccgagctgt tcggcgccgt
     2101 accggcggaa gaaccacgaa gtcgtcgtac ggggcaccgc ccagtacgcg gcggccagag
     2161 ccaccaggca gaccgggacg gacgcgatgc gcatggcgcg gctgcgcacg aagtcgaagt
     2221 gctcgggacg cagctggagg cccaggacgt agtacgggag gaactggagg atccgctgga
     2281 ggtgcaggtc cccgccgatg ttgctggtca ccgctcccat ggccgcgatg accagggaga
     2341 ccgccacggg caggcggacc gtctgccaga agggggtggt gaggcgccag aggaacaggg
     2401 cgggcaggaa ccacagcagc cagtacgggg tgagcgggct cagggggaag gtgggggtgc
     2461 cggtcaggcg ctcgaagagg gtgtaggcga tctcgaacac cgcgtagggc acggccaggc
     2521 tggtgacgag cttccacacc cggtcggacc gcagttccag gccgcgcgag aagtatccgg
     2581 cgatgatgat gaagaccggc atgtggaagg tgtagacgaa ggtgtagaac gtctccacca
     2641 cgcgaccgct gccgggtagg ggttcccacg agtgccccat cgcgacgagc acgatggcca
     2701 ggtacttcgc gttgtcgaag aacgggtcac ggccggggcg ggacggtgcg gttgccggtg
     2761 ctccggcccc ggcggcggcc ggtcccggcg ggcgtgaggt cagggagggc atcgggccac
     2821 cctaagggct gcccccggtc cccggcgggc gcaggacgat ccccggcggg gacacgggaa
     2881 cagccacggc acctcgccgc gctcctcgga acgcccggta cgtccggcgc gcggtcgtcc
     2941 ccgcgcctcg gggcgcgccg cgtccgacgc cgcgcccgga tccacgaggg acaccgggac
     3001 ggccctcgat ccggccgggg cggctcgggg gatcggggat cagggggtca ggggatcagg
     3061 gggtcagggg gtggggtcag gaccagcttc ccggtggtcc ggcgcgcgag gaggtcctcg
     3121 tgggcgcgcc gggcgtccgc gagcgggtag ctgccaccgg tcacgggctt gagccggccc
     3181 gcttcgacca gggcgaacag ctccctcagg gggccgccga ccgcgcccgg agcggcaagg
     3241 gcggggcgta gccagaatcc gacgacggag gcgttgagac ggcccagccg gcccgggtcg
     3301 acaggggtga agccggtgcg ggcggcgttg ccgtaggtca cgagccggcc gaaggaggcc
     3361 aaggagtcca gggcctggtc gaagaggtgc ccgccgacgg cgtccaggac gacgtcggcc
     3421 ttctccggga gggggtacgt gatcaccgcg tcggcgccga ggtccaggac gagccgttcc
     3481 ttggccgggg tggagacctg ggcgaggacc cggcccgccc cgaactccct ggccagctgg
     3541 acggcgaggc tgccgacgcc acccgccgcg gagtgcacga cgacgctctc gccggtgcgc
     3601 agacgggccg cggaccgcag caggtgccag gcggacagtc cctgcaccat gagggcgagc
     3661 gcctgggcgg caccgagctc ctcggggatc tccaggacgc cggactcctt ggcggtcacc
     3721 tgctccgcgt acccgtgcga gacgcgggcc agcacccgcc tgccgtccgg ggtgcggccg
     3781 acgacctcgc tgcccgggac gtgggggagg gcatcggcgc cgagatagga accgtccacc
     3841 tggtgggtgt ccgcgaagtt caccccggcg gcctcgacgg tgaggagcac ctctccgggc
     3901 ccgggctcgg gaggggccat ccgcacggga cggagtactt cgggaccacc gaagcggtcg
     3961 aactggatgg cgagcatgcc gcacgatacc gcggggtgat cgaggcccga gggggtctcg
     4021 cgacccgggc agctccgcac aggccgggac aaaacagtac gtacggtttg ttttgatggt
     4081 cgcccccggt cgccgtccca cgggtgcgga acgggccgcg taccgccgga tcgtcccggc
     4141 ttcccgtacg caccggagcc gtgggcgtgc cggccgggac cgagcggtac gcgggcggcc
     4201 gtgtccggga ccggcctgcc acactgcggc catgagcgat cttctccacg acagagtcgt
     4261 cctggtcacc ggggcgagca gcggtatagg tgcggcggcg gcgcgggtgt tcgccgagga
     4321 aggtgcgacg gtcgtcctgg ccgcccgcag ggaggaccga ctggcctcac tggtggccga
     4381 gttgaaggac aagggagccc aggcctccta cgtcgtctgc gatgtcacgg tcgcgtcgga
     4441 cgccgcccgg gccgtcggct tcacggtcga cacctacggg aggctggact cggccttcaa
     4501 caacgcgggg ctcggaggtg accggactcc cctgcacctc atgggcgacg acgtctacga
     4561 cgccgtgatg gacgtcaacg tgcgcggcgt ctggaactgc ctccggcacg agatagcggc
     4621 catgctgcag aaccccggca actgctccat cgtgaacaac agtagtgtgg gcgggctggt
     4681 ggccataccg gcggccgcgc cctacatagc ggccaagcac gcggtcgtcg gcctcaccag
     4741 ggccgccgcc gacgaatacg cccaacaggg gatcagggtc aacgcggtgg cgccgggcac
     4801 cacgcgcagt gagatcaccg ccgactggtt cgggcgcaac ccgggactcg aggagatggt
     4861 caacagcctt acgccgcagg ggcgtacagc cgaaccggag gagatcgcca acgcggccgc
     4921 ctggctgctc agcgaccgct gccccttcct cacgggcacc gtcatgccgg tggacggcgg
     4981 cttcgtcaac cagtgagccc tcagccgcag ggaccgctca gccggctccg gaagaccgtt
     5041 tctccgtcct gtgtcgctgt gaccagtacc gaggtctctc cgggggcggc gggcaggatc
     5101 tccgcctcga cccagcaggg gcggtcgaac tcggcgtacc gttcgaaggt gctggtgacg
     5161 gtggtcgcct cgaagcgcgc ggggtgaacg gcggcgtgcg cggcctggtc ggcagcctcc
     5221 atcagggcga gcccggggac gtggtcgacc gggtggtcgt acagcacggc gttgtcgacg
     5281 tcgttgcgca actgccagtg ccgggggcgg tccccggccg ccaggacgac gtcggccggg
     5341 gtggaccggc ccacggtgcg ggggtccacc ggagcgggca gcggccacgc gttccagtcg
     5401 acggtgaagt gttcgccgcg aagccggcgg tagacgcgcg tggagagcca gccgaactcg
     5461 gaatccgcgc ggaccgccac gctgccacct cggaggatgc ggaattccat gtcgagtgaa
     5521 ctcaccttcc tcccgcgctc cttggcgttc ctgaccgtga cctcgacggt gaggtcgcag
     5581 gcaccctcgg aggggacttc gaacccaggg gtgacggtga agtcgaagta gttcagcaag
     5641 gtgtggtcgg agaggggcgc gccgtacgcc gcgtgcgcga tggtcaggcc gctctgccgg
     5701 acggtctggg tgagcatccg cgggtcgtaa gggaggccgg cagggggagc cggccacctc
     5761 gcggacaacg agaactcgtc atcttccctc cgtcgccacg aggtgaggag gaccatgtcg
     5821 gtacgtccga ggtggacgta ctccttgcgg atgccgtccc gtccctcagc tgcggagagc
     5881 ggcgggcacg gcacggcggg tatgtcgggc acggccctcc cggacatggc gcaaaatggg
     5941 tattacggcg ctgatatgat accggttcac cggtactttt tgaagtagcg tcatgcaacg
     6001 ttcaaagggg agcgcgagtt ggcacgacag caacgggcga tccgcacgcg ccgaatcttc
     6061 ctgcaagccg ctgccgaggt cttcgacgag cacggctacg acgccgccac catcgccgcc
     6121 atcctcgacc gggccggtct cacgcggggg gccctctact tccacttcac gtcgaaggag
     6181 gagctggccc gtggtgtcct ccaggaggcg gtgacgtcgg acggggtcac cccgcagacc
     6241 ttcaagctgc aggagtgggt ggacatggcg ctcctgctcg cctaccggct gccccgggaa
     6301 cccctgctca gcgcctcgat ccggctgtcc gtcgacccgc gtgcgcgcgg gctcttcggc
     6361 acccgctggc cggactggat cgctctcggc acggagatgc tgaccgaggc caaggagcgc
     6421 ggcgagctcc ttccgcacgt cgatccggag gccacctccc ggctggtcgt cggggcgtgg
     6481 accggggtgc agctggtgac cgagtccatg gccgagccgc cggacctggt cgccgagata
     6541 tccgcgctct tcgagctcat cctccccaac aacgccgtcc cgggcgtgct ggcccgcctc
     6601 gacacctccc cgcaccgcgc ggcacgcatc atggagcagc agcgggcggt cgccgcttcg
     6661 tgaccgcccg gccccggcca cgggacgcgc ggcgccgtgt cacccgactg ggtgcgggtg
     6721 cctgcgacgt ggctggtcag cgcgcatggt ggccctatgt ggcttcgccg aacagccgtg
     6781 tgcgccacgg tcgtgccgct ggcattcctg gtgttccggg acgcgccggt acggctcgcc
     6841 gcgcccgagc aggccaccgc gaagccggcg acgccggccg gcgcccagag gcccgcgatc
     6901 gtcacgcggg cgcagtggca cgcggacgag agcctggtga aggaggacgc ggtgtacacg
     6961 ggggtggtga aggccgtctt cgtccatcac accgggcaca ccaacgcgta cgactgcgcc
     7021 gacgcccccg agatgctccg ggcgatggag gaggcgcacg tccggggcga gggctgggac
     7081 gacatcggct acaacttcgt cgtcgaccgg tgcgggacca tatacgaggg cagggcggga
     7141 ggggtcggtc gctccgtgcg cggtgcccat gccaccgggt tcaacgcgca cagcgtgggg
     7201 atcgcggccc tcggcacctt cgaccccggc gtaccggtgc cgaaggcgct catcgagggg
     7261 atcgcgaagg tgggtgcgtg gaagctggac cctgccgtcg atcctggggg catcgtgcgc
     7321 atggtctcga cgaacgacga gagcctctac aggcgcggcg aggtggtcta cctccatgcc
     7381 gtggccggtc accgtgacac cttccagacg tactgccccg gtgaggcgct ctatgcgcaa
     7441 ctgcctgccg tccgtgcgga gatggccaga ctgcgggccc tgaaggggta gcgcctaccg
     7501 tgcgacgtac tgggtgagga ccgcctgcac ctcgtagatg tcgacgccct tggtgaaggt
     7561 cttctggatc ggggccgggg agccggagac ccagatcttg agctccgcgt ccaggtcgaa
     7621 ggtccccgcc gtctccacgg cgaagtgcgt gatgctccgg taggggaccg agtggtactc
     7681 caccttcttg ccggtcatcc cctgcttgtc gatgaggacg agcctgcggt cggtgaagag
     7741 gatcgtgtcg cggatcagga gatatgcggc gtgcacctgc tcgccgtgcc cgagaagccg
     7801 tgcgtagtca cgctgcgcag cagccggatc gacggagtgc gcgttgccga acagtgccat
     7861 gagaagccct ccacccgagt gaatgtgcgg cctttgaacg cgtcaaagcc caaggaaaag
     7921 attatcggcg gcttatctgt tcgtgccctt gcacaccttc tggagcacag cggaggaata
     7981 ccgtcatgcc ggcatcaccc ctcgccaagc ggcccgtcga atcggtgctc gcacttctgc
     8041 tcgtcctcct gagcgtgctg ggcctctccg gccgcagcgc cgcggacgcc ccggcccagg
     8101 ctcccgccca ggcggtaccg gcccaggccg tcgcggccca ggcggtaccg gcccaggccg
     8161 tcccggcccg agccgtcatc gcgccgaccg tcacggcggg tacggactgg gaccgcatcg
     8221 ccgagtgcga gagcaacggc cgctggaaca tcaacaccgg caacggctac cacggcggcc
     8281 tgcagttcgc gccctccacc tggcgggcct acggcggcgg gcagtacgca ccccgcgccg
     8341 acctcgccac caggtccgag cagatcgccg tcggtgaacg cgtcgcccgc tcccagggga
     8401 tgggtgcctg gcccacctgc ggccgcctcg gtgcgaacgg tacgtcgaac tcctcgtcgt
     8461 cctcctcgtc ctcgtcgtcc tcgtcgtccg acggcgccga ccggagcgac gcctcgcagg
     8521 cggctcctca gcggcagtcg gccggcagtt cgaacagtgg ctcgtccggc gcttcgtcgt
     8581 cggccggcag ccaggccggc agcacgagcg gcagctcccc ccgcggtgac agcggcgtga
     8641 cgcaccactc cgaggacctg gccgacgcgg agacgtacgt cgtcgagtcg ggagactgcc
     8701 tgtcggtgat cgccgcccgc gcggacgtgt ccggtggctg gcccgcgctg tacgagctga
     8761 accgcgaggt cctggaccag gggccgcacc tcatcttccc cggtcagcgc ctccgtctga
     8821 acgcctgaac catgacggcc acgcctaccc gccgaaccgg cgctaccgga ccgtccactg
     8881 cgggccacgg cgttcctgcc cgtgccggga agcccggaca ggaccggacg gcggctgatc
     8941 cccggggccg ccgaacagca cagcaggcgg cacgacggac gcaacgacaa gcggcacagc
     9001 aggcggcacg acagatgaca cgacaagcgg cacgacaagc ggcacgacag gcggcacgac
     9061 aggcggcatg acagccatca cagcaggtgg cgcagcaggt cgcgggcgcg gatggcgtac
     9121 gcggacggcg gaccggcggc ctcatcgcgg cggaacggtc caggagggac accaggtggc
     9181 agacctcgcg tgggaacgcg gccgaaccga gcacggcgtc gcgccgggca aggagcgcgg
     9241 tgcggtcctg acgacggtgg cgcgatcggc gtacggagcg acatcgcagg gcgggcatcg
     9301 acaggacgct gtcggcgggc ccggccacgg cgctcaagcg gacagccggg cggccggccg
     9361 actcctcggc accacgccgg acgtcccgtg ggtcgccgaa agaggtgtca cggttcccgg
     9421 tggtgcatgg accacacgga accctgccgg acggacacgg cgaacggcac cccggccgca
     9481 ctcctcccct gctccggcgg cccaggccgg acccggtccg aaacctggcc cgggggccca
     9541 ccgctcaacg gcccacggag tccgcgtcgc gggcggtggc gggcgcgttc ccggattccg
     9601 cctggtgtcg gcggcagtcg tggttgtgac acggcccagg accccagtgc gggacgaaga
     9661 tgccgagggt cttgtgccgc gccagtacgg tcgcgacggg ctgcttgcag gccggacaca
     9721 cgtactcggt ctccgcctgc cgatcggtct gacgctcggt tctggccatg cttttacggt
     9781 agacctgaaa gggacaatta gaaacttttc tggcttttta cccgcccctg ctcccaggaa
     9841 cgggagacgc ccagacgaag ggaccaccgg tcatggcccg cgcaccgtca ccggccgcag
     9901 tcctgctctc cgtgcacgcg gtggtcttcg acacggacgg cgtgatcacc gactccgccc
     9961 cggcgcacgc cgcggcctgg aaagaggcgt tcgacgcctg cctggccctc cacgaaggcc
    10021 agcggccgtt cgacgagggg gacgactaca gacgccatgt cgacgggaag tcgcgcaagg
    10081 acggtgccgc cgccttcctc gcgtccaggg gtctccgcat tcccgagggc agccccgacg
    10141 acccgccggg caccgccacg gtgtgggccg tcgccgcaca caaggacgag gtgttcaccc
    10201 ggggcctgcg cacccacccg atcgaggtct ggccggggac ggtccgcctc ctgcgcgtcc
    10261 tgcacgacgc acgggtgccg ttcgccgccg cgtccgcgtc ccggcacgcg acggagctgc
    10321 tcgccggggc cggtctcctg gacctgttca cggcggtggt cgacggcaac gaggcgcgcc
    10381 gcctggagct gcccggcaaa ccggaccccg ccctgttcac cgaggcggca cggcgcctgg
    10441 gggtcccggc cggggagact gccgtcgtgg aggacgcgct ggccggagtg gaggcggggc
    10501 ggcgtggagg cttcgggctg gtcgtcgggg tggaccggac ggacagcacg gagggggccg
    10561 ccgcgctacg ccgtcacggc gccgatctcg tggtggccga tcccggggac ctgctgatca
    10621 acggggagga ctgatggcgc gcggctggat ctgggcgtac gaggggtacg agcccaggaa
    10681 ggagcgcctg cgggaagcac tgtgcaccct cggcaacggc tacttcgcca ccaggggagc
    10741 cgcgcccgag acccctgcgg gcgcgacgca ctatccgggt acctacgtgg cgggctgcta
    10801 caaccggctg acctcctccg tccagggacg ccaggtcgag aacgaggaca tggtcaacct
    10861 ccccgactgg ctgccgctgc gctaccgcat ccatcccgcc ggcgccggcg ccggacccgg
    10921 acccgga
//
