LOCUS       NZ_CABGTR010000006     11044 bp    DNA     linear   CON 13-OCT-2024
DEFINITION  Enterococcus faecalis strain 4928STDY7071622, whole genome shotgun
            sequence.
ACCESSION   NZ_CABGTR010000006
VERSION     NZ_CABGTR010000006.1
KEYWORDS    WGS; RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2019) WTSI, Pathogen Informatics, Wellcome Trust
            Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_902161995.1-RS_2024_10_12
            Annotation Date                   :: 10/12/2024 01:27:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,759
            CDSs (total)                      :: 2,695
            Genes (coding)                    :: 2,666
            CDSs (with protein)               :: 2,666
            Genes (RNA)                       :: 64
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 12 of 29
            Pseudo Genes (incomplete)         :: 16 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 3 of 29
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:08:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 74108
            Orig. end                         :: 85152
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CABGTR010000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11044
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11044
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11044
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6044](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6044)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(495..1082)
                     /locus_tag="FN307_RS13440"
     CDS             complement(495..1082)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1156: Chloramphenicol acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401939.1"
                     /locus_tag="FN307_RS13440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="WP_002401939.1"
                     /transl_table=11
                     /translation="MKVSIEDVINGYRSDAPVDLERDAYAEELYQEAIKIGIEMNTKYH
                     SEEELREIMSYLIGKKVDETFRIFPPFYTDFGKNITLGKNVFINSGTHFQDQGGIVIGD
                     GVFIGHNVVLATINHDLFPKNKRKNHYAPIVLKNNVWIGSNATITSGVTIGEWSVVAAG
                     AVVTKDVPPYTVVGGVPARVLKLIDKEEDGND"
     gene            complement(1085..1834)
                     /locus_tag="FN307_RS13445"
     CDS             complement(1085..1834)
                     /GO_function="GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365247.1"
                     /locus_tag="FN307_RS13445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_002401938.1"
                     /transl_table=11
                     /translation="MIEKQTAGRDVLGEFAPKFAELNDDVLFGQVWAREDKLSLKMRSI
                     VTITALISKGVIDSSLNYHLETARKNGVTKTEMAELLTHLSFYAGWPNAWAAFRLAKEV
                     YVEDHNKEEHGGLFGQGELNTAYSEYFIGQSYLKPLTDPKETVFIANVTFEPACRNNWH
                     IHHAKSGGGQILICVDGEGWYQEEGKEAQSLKPGDTITIPANVKHWHGAKKDRWFSHLA
                     IEVPGVETSNEWLEPVKNEFYDNLKEC"
     gene            complement(1848..2852)
                     /locus_tag="FN307_RS13450"
     CDS             complement(1848..2852)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365249.1"
                     /locus_tag="FN307_RS13450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_002401937.1"
                     /sec_met_domain="PF00561 (E-value: 1.3e-06, bitscore: 21.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTIGGKARMLEDTTMIQRWDKVFQKSDKVNHRKVTFTNRFGIAL
                     VADLYEPKRQAEEFPAIAVCGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGI
                     PRNVASGDINTEDFQAAVDFLSVQKNVAQDKIGIIGICGWGGMALNAAAIDTRVKATVV
                     STMYDMSRVTANGYFDSIDVDGRYEMRQQLNAQRIEDFKNGDYARSGGVPDSLSESAPY
                     YIKDYFDYYKTDRGYHKRSLNSNEGWNMTSALSLLNTKFLSYSDEIFSAVLIIHGEKAH
                     SRYFAEDAFKKLQGDNKELLIIPKATHTDLYDKIDIIPFDKMTEFFYQNLEVR"
     gene            2935..3375
                     /locus_tag="FN307_RS13455"
     CDS             2935..3375
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1171:
                     transcriptional regulator, MerR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002394295.1"
                     /locus_tag="FN307_RS13455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_002401936.1"
                     /transl_table=11
                     /translation="MTAYSIGEISKMFNIPVSTLRYYDKEGLFPEITRVSGIRKFSNNE
                     IEALRVIECLKKSGLEIKDIKQFMKWTKLGSETFKIRKELFERQKVSVEKELRQMEKVL
                     DMIKFKCWYYDEVLKQGNEVAVKSKIPNNLPKEVKLYYENSH"
     gene            complement(3731..4321)
                     /locus_tag="FN307_RS13460"
     CDS             complement(3731..4321)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002394296.1"
                     /locus_tag="FN307_RS13460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_002394296.1"
                     /transl_table=11
                     /translation="MKEGKAMIEIKDLNIPYVDKKISMNILKEYNLFTGDNGNGKTLLL
                     DYISGVKKAKRTVIKGNESIIYINQNIYFSDRLSGMDFLKFSYGLDGKKSIQSFYQLSD
                     KILKREDIDKLLKKQWGMLSGGEKKFLYALILMSLEREWYILDEPFAFVDKKRKKMLYK
                     IIDIKISEDKGIILTTHEEDEVVLKSVSKVFEI"
     gene            complement(4308..4988)
                     /locus_tag="FN307_RS13465"
     CDS             complement(4308..4988)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405757.1"
                     /locus_tag="FN307_RS13465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002365261.1"
                     /transl_table=11
                     /translation="MFISVKSYALFNIKMLLKDKIPLLWSIALPVITFFMNYRSVTNEW
                     ELAYWWVYIVVCSYVYGVGVYALQLKEYGSLKTIFSINNSPWAFFLGNLLTQIMFSFIS
                     VNLLNLIVVILLPLSFLRLFLYSFITIIACLPVAFFSYNLTLIKKVHANTINSIFTMLI
                     FGLFILLNTNIELNKYNPLSILSNVLIEHKNMDVFKYVTFSIFLVFISMYSIVKFEPIS
                     NERR"
     gene            complement(5001..6044)
                     /locus_tag="FN307_RS13470"
     CDS             complement(5001..6044)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401935.1"
                     /locus_tag="FN307_RS13470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_232047325.1"
                     /sec_met_domain="YcaO (E-value: 4e-08, bitscore: 26.2,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGFLRNKVNFVLNEDLFMSHHYNVYIEKYSKRPIKAYEVDLKNK
                     RQHILSTLGEYLERETLINENSPKEHTVIATNLIDGSIKQVNLDMILFKNSFVDSSGMA
                     SHKKSKPLIWTAYKEFFERQSYITNFLFHTKAQQLNIDKIEEIKKIDRYLTNYLDNVKY
                     YNISLSKNLYVVLAVGYGEKYKAVGLGTSQSARKAVIKSQKEILQYFATSHSKYKEGKV
                     SQGSGLVTDNKDIYHDYFDRLSSKEVKDLYAYLEGSEFIILNNEEKIEKVSHTFTIKNI
                     SNSLNMYPYITMFENNHAKNVKVVKVFDPKWFPNMAPRNFSKEIILNVENILKLKRKNF
                     SELLPFP"
     gene            complement(6062..7039)
                     /locus_tag="FN307_RS13475"
     CDS             complement(6062..7039)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365263.1"
                     /locus_tag="FN307_RS13475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002401934.1"
                     /transl_table=11
                     /translation="MIFTNKSSYGVKSDMFLSNINIGYVNFSRVKIKQGTTAGSAFSVD
                     ENKQLSLIKSYNEYLERLFLGIPLSTNKTISSVNLEKNIVSSRKYSEFGYGNHEFGFND
                     TTGTSSGKLSEVIIQKALTELIEKNEVFCFWYGLKGEKLKQDKEIHDLINDYNFISNMF
                     KIYVVRELSNYSTVIVMGFLEGKLLTSGVCCSITMEQSLILALKEAKGIEWQTFNNPLA
                     KANKFSDKLHKKILKKVKFMDEIYKVITKDSIQATKYIETADWINHVEISVIGDDINRG
                     VKTIKCISKQLLNSVPIKINIERQKDKEIIKRYLIDYSIDCPIQ"
     gene            complement(7023..7679)
                     /locus_tag="FN307_RS13480"
     CDS             complement(7023..7679)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401933.1"
                     /locus_tag="FN307_RS13480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002401933.1"
                     /transl_table=11
                     /translation="MSDYYWNEYKNVLLDEAYLIDTLKYQQRVVNKQVEENNLDELSDV
                     LGLYNVKYSDIRLAVYPFDELPVFIKTLVSDLQKNYASFIYKVSMPILAITKKIGNVNT
                     GCYSFDFKNNFLILYETDVTKIREIVNDKLVLAFFLNIEDAICLYKKKAYTNGIKEIGY
                     VSKSSQLAISKEKNIIINEMLVPEQQLTNGLGINLRKCLLMEALILEVLTNDIHE"
     gene            complement(7672..8502)
                     /locus_tag="FN307_RS13485"
     CDS             complement(7672..8502)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04424"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379875.1"
                     /locus_tag="FN307_RS13485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="McbB family protein"
                     /protein_id="WP_002365265.1"
                     /sec_met_domain="TIGR04424 (E-value: 1.1e-89, bitscore:
                     293.7, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYKVNPFIWHDLSEKETFFQTKLSSIIITNKNLVRMLKDLEKNEV
                     INFEESLLSNYFRSNMINNVLVFLEENKLIKKLDIENNKVNEVLVYTDDSEFSELFDYV
                     FSEKFTISHQSSISHLESKNMDGKLLIVFLKAFNFKEYENIVNIAKEKNVVIKIIFSYN
                     HHIYFSNFYKREWYNPCPICFFSELESQLRGEITSDTANFQTIIDLLYTKSQNFAIEYP
                     LQKDDYFTIVYTIMRNLDSDLMKCDLDEVLSIDLNNHLVTKDIAYHWGYCDCYE"
     misc_feature    complement(8543..8554)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            complement(8567..8767)
                     /locus_tag="FN307_RS13490"
     CDS             complement(8567..8767)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405754.1"
                     /locus_tag="FN307_RS13490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002365266.1"
                     /transl_table=11
                     /translation="MNVEEVLVSQKTPNAINEETELYGFGYGSVVENITVSKGGGGGGG
                     GGGGCCCCCCCCCGGATGLEE"
     gene            complement(9462..10775)
                     /locus_tag="FN307_RS13495"
     CDS             complement(9462..10775)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /locus_tag="FN307_RS13495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NCS2 family permease"
                     /protein_id="WP_002355107.1"
                     /transl_table=11
                     /translation="MKEKISSYFELEQLNTNMKREMLAGFTTFISMAYILFVNPTVLGA
                     SGMDEGAVFTATALASALGCILMGVLAKYPIATAPALGINAFFAYSVSVGMGIPWQTTL
                     AGVFVASLIFILITIFKLRELIIDAIPADLKFAISGGIGLFIAFLGLSQGGLIVANDAT
                     LVGLGPLNVGSTWVTIVGLVVTAILLVRRVPGGIFIGMAISTIVGLATGVIPMPDKIIS
                     SAPSLEPTFLVALGHVKDINTIQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMKDNKMP
                     RVGKALAADSTAMLAGSLLGTSPVGAYVESSAGIAVGGRSGITAITTGILFIFSLLFSP
                     LLAVVTSQVTAPALIIVGVLMAQSLSKIKWNEMEIAIPSFLILLGMPLTYSISDGIALG
                     FIFYPITMIAAKRGKEVSPIMYGLFFVFVGFMWILNAQ"
ORIGIN
        1 atagatacat agctccccca tggctaactg ccaaaacgtt ctgattgttt tcttgttcca
       61 taattttagt aagtccttcg tttattcttt cctgcaccac ttttgaagac tctccgccat
      121 aaggcacaaa agaatcacca tatgaagttt ccccaatagg tcttttaggg tttaaatatt
      181 ctctttctcc ttcaaatata ccaaaattcc attcttttag ttccttcatt cgcttatatt
      241 ttttatttcc tataattatc tctagtgtat ctgatgcacg ctcttgtgta gaggaataat
      301 aagcatctaa tttaatatta cgattttgga agtactcttt tgcaattctt gcttgtttta
      361 ttcctacttc agtcaaaggt gagtcacttg ctccttgaat tttccctaat ttattaaata
      421 gcgtttcacc atgtcgcata agataaagtt tcttcatatt atcagtctca tctgcttttg
      481 actttgttgt tttatcaatc attaccatcc tcttccttat ctattaattt tagcacacga
      541 gcgggaacgc cacctactac agtgtagggt ggaacatctt ttgtgacgac cgcacctgct
      601 gcaactactg accactcacc aatcgttaca cctgaagtaa tcgtagcatt tgaaccaatc
      661 catacgttat tttttaacac aattggcgca taatgatttt tacgtttatt ctttggaaat
      721 aggtcgtgat taattgtagc aagtactaca ttatgtccta taaatacacc atcaccaatc
      781 acaatcccac cttggtcttg gaaatgcgtc cctgagttta taaagacatt tttgccaagt
      841 gtaatgtttt taccaaagtc cgtataaaaa ggtggaaata tacgaaaagt ttcatctact
      901 ttttttccaa tcaagtaact cataatttct cgtaattcct cttctgaatg atacttggta
      961 ttcatttcta ttccaatttt tatcgcttct tgataaagct cctcagcata cgcatctcgc
     1021 tctaaatcaa caggagcatc acttctgtac ccattaataa catcttcaat acttactttc
     1081 attattaaca ctcctttaga ttatcataaa attcattttt aacaggctct agccattcgt
     1141 ttgatgtttc taccccaggc acttcaatcg ctaagtggct aaaccaccga tctttctttg
     1201 caccgtgcca atgctttaca ttggcaggta ttgtaatggt atcccctggc ttcaggcttt
     1261 gtgcttcttt tccttcttct tgataccaac cttcaccatc tacgcatatc aagatttggc
     1321 ctcctcctga tttcgcatga tgaatatgcc aattattacg gcaagctggt tcaaacgtga
     1381 cattggcaat aaacactgtt tcttttggat cagtcaatgg tttcaaataa ctttgaccaa
     1441 taaagtattc tgaataagct gtgtttaatt ccccttgacc aaacaatccc ccatgttctt
     1501 ctttgttatg gtcctccaca tacacttctt tcgccaatcg aaacgctgcc caagcattcg
     1561 gccatcctgc ataaaatgaa agatgtgtta ataattcagc catttctgtc ttagttacac
     1621 catttttccg agcagtttcc agatgatagt tcaaagatga atcgataact ccctttgaaa
     1681 tgagcgccgt tattgtcaca atagaacgca ttttcaaaga aagtttatct tctcttgccc
     1741 atacttgacc aaacaacaca tcatcattca gctcagcaaa tttgggtgca aattcaccta
     1801 aaacatctct tccagcagtt tgtttctcaa tcatttacaa attcctccta tcttacttcc
     1861 aaattttgat aaaaaaattc ggtcatttta tcaaatggaa tgatgtctat tttgtcgtag
     1921 agatcagtgt gtgttgcttt aggaataatt agcaactcct tgttatctcc ttgcaacttc
     1981 ttaaaagcgt cttctgcgaa atatcgagaa tgagcttttt caccatgaat aattaacacc
     2041 gcactaaaaa tctcatcact ataagacaga aatttagtat ttaacaatga aagagcagac
     2101 gtcatattcc aaccttcatt tgagttcaac gatcgcttat gatagccgcg gtctgttttg
     2161 taataatcga aataatcttt aatataataa ggcgcacttt ctgacaaact atctggaaca
     2221 ccacccgaac gagcataatc cccatttttg aaatcttcaa tacgttgtgc attgagttgt
     2281 tgacgcattt catatcgacc atctacatca atagaatcaa aataaccatt agctgttaca
     2341 cgagacatat catacatagt cgaaacaacg gttgctttta ctcttgtatc aattgcagct
     2401 gcatttagtg ccatgccgcc ccaaccgcaa ataccaataa ttccaatttt atcctgagca
     2461 acattttttt ggaccgacaa gaaatcaaca gccgcttgga aatcctcagt gttaatatcg
     2521 ccagaagcaa cattacgtgg gatccctcca ctttctccag taaatgacgg atcaaaagct
     2581 atcgtcaaaa agccccgttc tgccattgtt tgagcataaa gtcctgatga ttgttcttta
     2641 actgccccga acggaccgca cacagcaatt gcaggaaact cttccgcttg tctttttggt
     2701 tcatataaat cagcaactag agctattcca aatcgattag taaaagtcac tttacgatga
     2761 ttaaccttat cgcttttttg gaatacctta tcccatcttt gaatcattgt agtatcttct
     2821 agcatccttg ctttccctcc tatagttgac atttaatttt tttaataata caatcttact
     2881 acctaaactt aactttaggt caagtctgat tataaaaatt tttcagaagg agctatgaca
     2941 gcatattcaa tcggcgaaat atcaaaaatg tttaacattc cagtttcaac tctccgctat
     3001 tatgataaag aaggattatt tcctgaaata acgcgtgttt ccggtattcg aaaattttct
     3061 aataacgaaa ttgaagcttt aagagtgatt gagtgtttga agaaatctgg attagaaatt
     3121 aaagatatta aacaatttat gaaatggaca aaattagggt cagaaacttt caaaattcga
     3181 aaagaactat ttgaacgaca aaaagtaagc gtagaaaaag aattgcgaca aatggaaaaa
     3241 gttcttgata tgattaaatt taaatgctgg tactacgatg aagtccttaa gcaagggaac
     3301 gaggtggctg ttaaatcaaa aattccaaat aacttaccaa aagaggtaaa actttattac
     3361 gaaaactctc attaatttga aacgattgta catttaatga gttaatctaa acaaaaaaca
     3421 aacgttattt aaattagtag agtagatttg tcactagcac tttaattttc atagactaca
     3481 ctatttaaat aagttcaaaa agcaacaact gctagcagca gagctaacaa tcgttgcttt
     3541 ttgaacttat ttactataca aacaaaaaca ttaaaaacca gtattatcta tactttccaa
     3601 aaaaactact atacaaaaac ataattattt tatatttact ataatttaaa ctacagtctg
     3661 agcttattat aattcattct tttatcgtac aacattaaat attctaaaat gatgacacta
     3721 cttcttacag ttatatttca aaaaccttgc taactgattt taatactact tcatcttctt
     3781 catgagtagt taaaataata cccttatctt ctgatatctt aatatctata atcttatata
     3841 acattttttt tcttttctta tctacaaaag caaacggttc atctaaaata taccattctc
     3901 tttctaaaga catcaaaata agagcataaa ggaacttttt ttcccctcct gaaagcattc
     3961 cccattgttt tttaagcaac ttatcaatat cttcacgctt aagaatttta tcgcttaatt
     4021 gataaaaaga ttgaatactt ttttttccgt ccaaaccata actaaatttc agaaagtcca
     4081 ttcctgataa acgatcagaa aaatatatgt tttgattaat atatatgata ctttcattcc
     4141 ccttaattac agttcttttt gcctttttta ctcctgaaat gtaatctaat agtaaggttt
     4201 ttccgttacc attatcacca gtaaataaat tatactcttt taaaatattc atacttattt
     4261 ttttatctac ataaggaata tttaaatcct ttatttcgat catagcttta ccttctttca
     4321 tttgaaatcg gttcaaattt aactatagaa tacatactaa taaaaactaa aaatattgaa
     4381 aaagtaacat atttgaagac atccatattt ttatgttcaa ttaaaacatt agataaaatt
     4441 gacagagggt tatatttatt taactctata ttcgtattta acaatataaa taaaccaaaa
     4501 attagcattg taaatattga atttatagta ttagcatgca ctttttttat tagagttaaa
     4561 ttgtaactaa aaaatgcaac tggtaaacac gctataatag taataaagct atataaaaaa
     4621 agacggagaa acgataatgg tagtaaaata accacaatta aattaagtaa gtttacactg
     4681 ataaagctaa acataatttg cgtcaataga ttgcctaaaa aaaatgccca aggtgaatta
     4741 tttattgaaa aaattgtttt taagcttcca tattccttta attgtaatgc gtaaactcct
     4801 actccataaa cataggaaca aacaacaata tatacccacc aatatgccaa ttcccattca
     4861 tttgttacac ttctataatt cataaaaaaa gtaattacag gtaaggctat tgaccacaat
     4921 aaaggaattt tatctttcag taacatttta atattaaaca atgcatatga tttaactgag
     4981 ataaacattt tcactcctcc ttacggaaat ggtaacagtt ctgaaaaatt ttttcttttt
     5041 aattttaata tattctctac atttaatatt atttctttcg aaaaatttct tggtgccatg
     5101 ttggggaacc actttgggtc aaataccttc actactttca cattcttagc atgattattt
     5161 tcaaacattg taatatacgg atacatatta agagaattac ttatattttt gatagtaaat
     5221 gtatgactaa ctttttctat tttctcttca ttattcagaa taataaattc actaccttca
     5281 agatatgcat ataaatcttt aacttctttc gagctcaatc tatcaaaata gtcatgataa
     5341 atgtccttat tgtcagttac taatccagag ccttgactaa cttttccttc tttatatttt
     5401 gaatgcgatg tagcaaaata ttgtaatatt tctttttgtg attttattac tgcttttctt
     5461 gctgattgac tagttcccaa acccactgct ttgtattttt caccataccc aacggcaagt
     5521 accacgtaaa ggttcttact taaagatata ttataatatt tgacattgtc taaataatta
     5581 gtcaaatatc tgtctatttt ttttatttct tctatcttat ctatatttaa ttgctgagcc
     5641 ttagtatgaa acaagaagtt agtaatatag ctctgtcttt caaagaattc tttatacgct
     5701 gtccatatta atggctttga ttttttatgc gaggccatac cagaagaatc aacaaaacta
     5761 ttcttaaaca aaatcatatc aagattaact tgtttaatgc ttccatcaat taaattcgta
     5821 gcaataaccg tatgctcttt tggtgaattt tcgttgatga gtgtttctcg ctccaaatac
     5881 tctcctaaag ttgaaagaat atgctgcctt ttattcttta aatcaacctc ataggctttg
     5941 ataggtcttt tagaatattt ttctatatat acgttatagt gatgtgacat aaataaatcc
     6001 tcatttaaaa caaaattcac cttatttctt aaaaaacctt tcaaaagtat tctcctttct
     6061 atcattggat aggacaatca atagaataat caattaaata tctcttaata atctctttat
     6121 ctttttgtct ttcaatgttt atcttaattg gtacagaatt tagtaactgt ttggatatac
     6181 attttattgt tttaactcct ctatttatgt catctccaat tacggatatc tcaacatggt
     6241 taatccaatc agcagtttca atgtatttcg tagcttgtat agaatctttt gtaataactt
     6301 tgtatatttc atccataaac tttacttttt ttaaaatttt tttatgcaat ttatcagaaa
     6361 atttatttgc ttttgctaag ggattattaa aagtttgcca ctctattccc ttagcttctt
     6421 tcaaggccaa aattagagat tgttccatag taatactaca acatacacct gaggttagta
     6481 atttcccttc taaaaacccc atcacgatca ctgttgaata attactaagt tcacgaacta
     6541 cataaatttt aaacatattt gaaataaaat tataatcatt aattaagtca tgaatttctt
     6601 tatcttgttt taacttttcc cctttcaaac cataccaaaa acaaaatact tcatttttct
     6661 caatcaattc tgttagtgcc ttttggatta ttacttctga aagttttcca gatgaagttc
     6721 ctgtcgtatc attaaatcca aactcatgat ttccatatcc aaattctgaa tattttctgc
     6781 tacttacaat atttttttct aaatttacag aagaaatagt tttatttgta cttaatggta
     6841 tccctaagaa aagtctttct aaatattcat tataactttt aataagtgat aactgtttat
     6901 tttcgtcaac tgagaaagca ctacctgctg tcgttccttg cttaatcttg acacgtgaaa
     6961 aattcacata tccaatattt atatttgata aaaacatatc acttttcaca ccgtaggaac
     7021 ttttattcgt gaatatcatt agttaacacc tctaaaatta aagcttccat taacaaacat
     7081 tttctcaagt ttatacctaa tccatttgtc agttgttgct ctggtactag catttcgttt
     7141 attattatgt ttttttcttt actaatcgct aattgactgc ttttagaaac ataaccaatt
     7201 tcttttatac catttgtgta cgcctttttt ttatataaac aaattgcatc ctctatgttt
     7261 aaaaaaaaag ctaataccag tttatcattt actatttctc taatcttagt tacatcagtt
     7321 tcatatagaa tcaaaaaatt atttttaaaa tcaaaagaat aacaaccagt atttacattc
     7381 cctatttttt ttgttatggc tagtattggc atactaactt tatatataaa ggaagcataa
     7441 tttttttgta aatcactaac taacgttttt ataaagacag gtaactcatc aaaaggatat
     7501 accgctaacc taatatccga gtatttcaca ttataaagac ctaaaacatc gctcaattca
     7561 tctaaattat tctcttctac ttgtttattc actacccttt gttgatattt taacgtatca
     7621 attaaatatg cttcatctaa tagtacattc ttatattcat tccaataata atcactcata
     7681 gcaatcacaa tacccccaat gataagcaat atcttttgta actaaatgat tatttaaatc
     7741 aatagataaa acttcatcta agtcacattt cattaaatcg ctatccaaat tcctcattat
     7801 ggtataaact atcgtaaaat aatcatcctt ttgcaacggg tattctatag caaaattttg
     7861 tgattttgta taaagtaaat ctataattgt ctgaaaatta gctgtatcag aagttatttc
     7921 tcctcttaat tggctttcta gttcagaaaa aaaacatatt ggacatggat tgtaccattc
     7981 ccttttatag aaattcgaaa aataaatatg atgattatag ctaaaaatta ttttaatgac
     8041 tacattcttt tccttggcta tatttacaat attttcatat tctttaaaat tgaaagcctt
     8101 taaaaatact ataagcaatt taccatccat atttttagat tctaagtggg aaatactgct
     8161 ctgatgacta attgtaaatt tttcggaaaa cacgtaatcg aataattcag aaaattcaga
     8221 atcatcggta tataccaaaa cctcattaac cttattattt tctatatcta gctttttaat
     8281 taatttattt tcttctaaaa agaccagtac attattaatc atgttgctcc taaaataatt
     8341 ggataataga gattcctcaa aatttattac ttcatttttt tctaaatctt ttaacattct
     8401 aactaaattt ttattagtaa taattatcga agatagtttt gtttgaaaga atgtttcttt
     8461 ttcactaaga tcatgccata taaaagggtt tactttatac attttttcac tcctttttac
     8521 ttgagtatgt aaagaggtaa gtgcgtccac ttaccttatg aagtttctat tcttctaatc
     8581 ctgtggcacc cccacaacaa cagcaacaac aacaacaaca gccaccgccg ccgcctccac
     8641 caccgcctcc acctttagag acagtaatgt tctcaacgac agagccatac ccaaacccat
     8701 ataattcagt ctcttcatta attgcatttg gggttttttg cgacactaaa acttcttcaa
     8761 cattcatttt aatacacctc cgttataatt ttatcaatat tgatgaaatt attttaccac
     8821 tatccaatca tttttcaagc aaaaactcat cttattctaa ttaaaaaacg tatttttata
     8881 ctgaaaaatc taaaagtctt tgattagaca acaaaaacaa aaaaattaat aacaaaacaa
     8941 ctatcattaa tttttctatt tttatatcaa tgaaaattct aacaaaaata atttttaaaa
     9001 tatttatttt atcacattaa aattataata tacaatgcat attcgcatat tttcggattt
     9061 tacctcttta ataacaattg tgaataccta gttttatgaa ttgatactta ctataaggaa
     9121 agcatattgt ttaataacaa aacatattta agtatttaaa aagcaacaac cgctaacaca
     9181 agagctaaca atcattgctt tttgaactta tttactgtac gaaaaaagac attaaaaacc
     9241 agtattctca atacatttca acagaaacta ctacctgaaa tcataatttc tgtatacttc
     9301 actcaattaa ttcctctctg ttcctgtatc ttcacaggtc aatgtctttg caaattatac
     9361 atcaaataag gggcgttttt ctagtgctta gtcgaaaaaa aatagttgat tcataagttc
     9421 attgtcttat gaatcaacta tttgataact ttctctcttg cttattgcgc attcaggatc
     9481 cacatgaatc cgacaaaaac aaagaacaag ccatacataa ttggcgaaac ttctttcccg
     9541 cgttttgccg caatcattgt aattggatag aagataaagc ctaaggcaat cccatctgaa
     9601 atgctgtatg tcaatggcat tcctaacaag attaagaagg aaggaatcgc aatttccatc
     9661 tcgttccatt taattttgct taaggattgt gccatcaaaa caccaacaat aatcaacgct
     9721 ggtgctgtaa cttgcgaagt gacaactgct aacaaaggag aaaatagcaa actgaaaata
     9781 aataaaatcc cggttgtaat agctgtgatt cctgaacgac cacccacagc aatccccgca
     9841 gatgattcga catatgcgcc aactggcgat gtccctaata gagaaccagc taacatcgct
     9901 gttgaatcag cggctaaagc tttccccaca cgcggcattt tattgtcctt cataaaacct
     9961 gcttgattgg ctaagcccac caacgtacca gcggtatcaa agaatgttac taataggaac
    10021 gttaaaacca cgacccacat ttgaatcgta ttgatatctt tcacatgtcc taacgccact
    10081 aaaaatgttg gttctaaact tggcgcgctt gaaataatct tatcaggcat tggaataacg
    10141 cctgtcgcta aaccaacaat cgttgaaatt gccatcccaa taaaaatacc acccggtact
    10201 cgacgaacta acaaaattgc agttacgact aagccgacaa tggtaaccca cgttgaacca
    10261 acattcaaag gtcctaagcc aacgagtgtc gcatcattag ccacaattaa gccaccttgg
    10321 cttaaaccaa gaaaagcaat aaacaacccg atcccacctg aaatggcaaa tttcaagtct
    10381 gcaggaattg catcaataat tagctcgcgt aatttaaaaa ttgtaattaa aataaatatc
    10441 aatgaagcca caaaaacgcc tgctaatgtc gtttgccaag gaatccccat accaacagag
    10501 actgaataag caaagaatgc attaattcct aaagctggtg ctgtcgcaat cggatatttc
    10561 gccaacacac ccattaaaat acaacctaag gcactcgcta aagcggtggc agtaaaaaca
    10621 gcgccttcgt ccatgccaga agcaccaagt acggttggat taacgaacaa aatatacgcc
    10681 attgagataa acgtggtgaa accagctaac atttctcgtt tcatattcgt attcaattgt
    10741 tcaagttcaa aatatgaact tattttttct ttcatgttcc gtcctcctaa aatctaaata
    10801 ttttacaatg ttcgtaaaat attcaattta ttaatgaaac aacgtgttta atcatataat
    10861 aaaccaacaa ataagtaaat atcaaattct aaattctgta aaaaacgaac aatcacgatt
    10921 taaaaaaagt attcctttct aggaatactt tttaaattaa atcgtttgtg tggtcgggcg
    10981 aacaaccatt tcactgattg acatacgatc tggcgtgtct atggcaaaga ccactgcttg
    11041 cgca
//
