LOCUS       NZ_CAAHQE010000012     11338 bp    DNA     linear   CON 19-DEC-2024
DEFINITION  Streptococcus pyogenes strain Bel002, whole genome shotgun sequence.
ACCESSION   NZ_CAAHQE010000012
VERSION     NZ_CAAHQE010000012.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2019) WTSI, Pathogen Informatics, Wellcome Trust
            Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900985135.1-RS_2024_12_19
            Annotation Date                   :: 12/19/2024 10:09:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,745
            CDSs (total)                      :: 1,683
            Genes (coding)                    :: 1,623
            CDSs (with protein)               :: 1,623
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 28 of 60
            Pseudo Genes (incomplete)         :: 32 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 11 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:22:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 11338
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CAAHQE010000012.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11338
                     /db_xref="taxon:1314"
                     /isolation_source="skin infection"
                     /mol_type="genomic DNA"
                     /note="contig: ERS1106169SCcontig000012"
                     /organism="Streptococcus pyogenes"
                     /serovar="EMM186.2"
                     /strain="Bel002"
     region          1..11338
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /tool="antismash"
     cand_cluster    1..11338
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11338
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[4781:7565](-)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(4782..7565)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     misc_feature    152..170
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 25.27"
                     /tool="antismash"
     gene            complement(171..908)
                     /locus_tag="E9931_RS08790"
                     /old_locus_tag="SAMEA3919035_01722"
     CDS             complement(171..908)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568302.1"
                     /locus_tag="E9931_RS08790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01722"
                     /product="ABC transporter permease"
                     /protein_id="WP_023612112.1"
                     /transl_table=11
                     /translation="MNDYFKAEWLKTRKGAILAVGIIFLVLSSFIGLANFFLNYEVLIE
                     EQMSRVLWGQLTFYNSQLLFPAMLAIFSGMIILPEFERKTFEMLRANQVSVKKMLLSKF
                     LLALLLITVIQVLLFVIYLVTLKLSQIPFVAKDLLLFLRATVLSVIGSSSVVMIHSYIM
                     AKTKNFSKSVGIAAIGSFCGFIFIMLGGVINQFFPYSQPMIGIRSRALVDMSISEFVIF
                     IVVNALYSFIFFKLTLRTLEKNG"
     gene            complement(901..1665)
                     /locus_tag="E9931_RS08795"
                     /old_locus_tag="SAMEA3919035_01723"
     CDS             complement(901..1665)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598724.1"
                     /locus_tag="E9931_RS08795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01723"
                     /product="ABC transporter permease"
                     /protein_id="WP_085634204.1"
                     /transl_table=11
                     /translation="MIKAIQIELLKSKRTKSFMISFIMMLVAVTWSLLAASRSIDIPKL
                     RTIGLFFNNLQANSIFLPIATCLFVSRIVGNEKEGSTFKLQEANNTNLIIIFTRKLIFT
                     NSVFLLLNIFQVLIVYLNVLRYDIQVPIWTLILQVVGLTMSSFTLITLFLYLSIILEKQ
                     GILLGIGFLSGFFGMITSQTLKWINLIFPFGGSSFLALYRIKILNSSDSLDYIFSWDKS
                     VPLNYTLYLIYCVMIYSVVKYLLKKKKGDNNE"
     gene            complement(1667..2575)
                     /locus_tag="E9931_RS08800"
                     /old_locus_tag="SAMEA3919035_01724"
     CDS             complement(1667..2575)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263495.1"
                     /locus_tag="E9931_RS08800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01724"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_085613744.1"
                     /transl_table=11
                     /translation="MKNAIELRKLSKVYQDITTVDLEKITVREGEIYGFLGPNGAGKTT
                     TMKMILSLVSPTLGEILINGEDIKGNHAYLSVIGSMIEEPSYYPNLTGYENLLVFQKMV
                     GFDESNIWPTLALVGLAEENNRKKLVKAYSLGMKQRLALAFALVKKPKILLLDEPTNGL
                     DPAGIHEIRELIVKLAKEKGLTVFISSHILSEIEHIADRVGIINHGRLVYEGEIEAIKS
                     NTWIEIGGDFSQNNIVQSLINYGQVEVLGASASHVKFKNIDNDKLADVVTYLIENDYRI
                     FRVVRESENLEDIFLSLTKEV"
     gene            complement(2687..4759)
                     /locus_tag="E9931_RS08805"
                     /old_locus_tag="SAMEA3919035_01725"
     CDS             complement(2687..4759)
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002268663.1"
                     /locus_tag="E9931_RS08805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01725"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_023612120.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 7.1e-41, bitscore:
                     128.7, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIILQNNEEDCLLACYTMLLNDLGHKVPLYEIYDKDTLPADGLN
                     VSYLLSLSDRFGVKINAYHASFDELLKVYGEKKQRMILHWNNDHFVVLEKITSNKTVIV
                     DPAIGRIKYSRDEFLTHYSETMVSVNKRNNFHPQTYKKIFWKYFKQTLQLKPIALFLLS
                     LLFIQVSVLLFSVILRQMLAEDFKFGISLFLLGAVLIFQLLGYFIKNGALDRYNTDFDK
                     YYSKELFQRLLQKPLLYFRNHLSGGVSEKISFKSTLRDNVTLKIIPSCVSLISAIIIFA
                     YLMTISIKLSVILILMIASYSIISTLLYHRQNEYNQTYLQYLIDFNSELQTDLDDIDYI
                     KIMRREKTVFHSWMEQNNQVTKKYSQILKIENLSQLIGTIFNYISLSSIIVIAVYYKDY
                     INLSLPDLLVYQTSISLLISAIEQVKGAVFEVIRLGVYAEKQSDLLKDTSPIVVPSSQS
                     DDYLIKTKNLNFSYGNKPIYSDVNLTISKGEKIAIVGKSGSGKSTLLLMLAGMLRYDGS
                     LEYGIEKFKDNLRVVLQNMTPRKGTVLENLEWTLDDVTPLYQVLNDTTADEVIARLPNK
                     IHSRLLKQGKNLSGGQIQKLLIAKSLLKENSIIFWDEAFSNLDEQSKNKIYDNVLQNNR
                     YSDRTMLIVSHHLDIVNYVDSIIFIDNETGKVIKDTHEHLMETNVNYTNFITSKN"
     gene            complement(4782..7565)
                     /locus_tag="E9931_RS08810"
                     /old_locus_tag="SAMEA3919035_01726"
     CDS             complement(4782..7565)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303301.1"
                     /locus_tag="E9931_RS08810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01726"
                     /product="type 2 lanthipeptide synthetase LanM family
                     protein"
                     /protein_id="WP_136293947.1"
                     /sec_met_domain="DUF4135 (E-value: 8.4e-79, bitscore:
                     254.9, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 1.4e-69, bitscore:
                     224.0, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNPKELLYSQFDRFPKVVIENNFPELLNESSELIKDVEDEISDYY
                     RSTLIYLINEKRIEKNLIGDSPESRYEYFNNVLCQNGLIFEEIDRRFPSINQRVMSTIK
                     KRLELINFVKERFTLDFKELRETGYIYSEAQTPKISEVKIKITGDIHNGCGVCILSYEE
                     QKVVFKKKSSNPNVLLHELNIEVGKFLQKDIDFIPDFLDKGEYFWEKFESSSPLRTEED
                     AKEFYRRMGYLLSYSYILNISDLHFENLISTSFSPKLVDVETVFSVSPYQTVANNESTL
                     EIINNSRNSILSTGLLPVSEAGKVFGGDTSGVLGGTLIGEAKIVINHNRDDIHVEKQKF
                     KTENQDHLPYFIDSKGMKEFLNAEDYVEYIKEGFREVSYFFMNSQDFLKKLYIKHNDIK
                     TRILFRNTRDYSLVRQLLVSPVYCEQSEILFETMANKLSEQNSRSLCLSEKKQLLNMDI
                     PYFYSNIDSCDIKDENMIIWNLESSALSEAINKLEKLSEEIINEQIELIEFSIKTPKAL
                     YSTELQEAYQKFEKVSSSENIIKTGIDTLVDIILENESNSLKDDSTNWLTLKVTDYDAF
                     ELVPMDDSLYEGLSGIAISLSEAYDFLDSGRQRRVKECLKRIFSVLSNSYMKLPNHSFF
                     VGKLGIYSALKRISVVTGQEIQNSIMNYNNLKYTLDVDVLSADFLSSFPNEITALRNSD
                     IKIDNLTQALDKLKELAIVQKDFISWDKLESNNVSLAHGNLGVEIALLYLAGKLESPEA
                     LNLFHKAKMFDKHQKLENGWIDKRNSSTSANWCHGSTGVLVARLAQLKLDDEYSLLSHS
                     ERIELENDMKHAAKQILEIGFDMTNFSLCHGTSGNLLALTYYQSYLTGADSEKLKEILD
                     REYRKLHSFGLENGWMCSFNTKYNVYGLMTGVSGILFSTVKYMKGDDSLDVLIPNF"
     gene            complement(7586..7741)
                     /locus_tag="E9931_RS08815"
                     /old_locus_tag="SAMEA3919035_01727"
     CDS             complement(7586..7741)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) leader_d"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794682.1"
                     /locus_tag="E9931_RS08815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01727"
                     /product="lacticin 481 family lantibiotic"
                     /protein_id="WP_023612118.1"
                     /sec_met_domain="L_biotic_A (E-value: 7.8e-27, bitscore:
                     82.6, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="leader_d (E-value: 1.8e-07, bitscore:
                     20.9, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEEKMCLGALNALQEFQIEELDNLLGGRGHGVNTISAECRWNSLQ
                     AIFTCC"
     CDS_motif       complement(7661..7741)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MEEKMCLGALNALQEFQIEELDNLLGG"
                     /locus_tag="E9931_RS08815_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(7586..7660)
                     /prepeptide="core"
                     /locus_tag="E9931_RS08815_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="3.80"
                     /molecular_weight="2594.9"
                     /monoisotopic_mass="2593.2"
                     /core_sequence="RGHGVNTISAECRWNSLQAIFTCC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEEKMCLGALNALQEFQIEELDNLLGG"
                     /leader_location="[7660:7741](-)"
                     /alternative_weights="2613.0"
     gene            complement(7941..8096)
                     /locus_tag="E9931_RS08820"
                     /old_locus_tag="SAMEA3919035_01728"
     CDS             complement(7941..8096)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) leader_d"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410306.1"
                     /locus_tag="E9931_RS08820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01728"
                     /product="lacticin 481 family lantibiotic"
                     /protein_id="WP_085613746.1"
                     /sec_met_domain="L_biotic_A (E-value: 2.3e-29, bitscore:
                     90.7, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="leader_d (E-value: 3.4e-08, bitscore:
                     23.1, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKEHEIINSIQEVSLEELDQIIGAGKNGVFKTISHECHLNTWAF
                     LATCCS"
     CDS_motif       complement(8022..8096)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MTKEHEIINSIQEVSLEELDQIIGA"
                     /locus_tag="E9931_RS08820_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(7941..8021)
                     /prepeptide="core"
                     /locus_tag="E9931_RS08820_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="5.20"
                     /molecular_weight="2778.2"
                     /monoisotopic_mass="2776.3"
                     /core_sequence="GKNGVFKTISHECHLNTWAFLATCCS"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MTKEHEIINSIQEVSLEELDQIIGA"
                     /leader_location="[8021:8096](-)"
                     /alternative_weights="2796.2"
                     /alternative_weights="2814.2"
     gene            complement(8127..8282)
                     /locus_tag="E9931_RS08825"
                     /old_locus_tag="SAMEA3919035_01729"
     CDS             complement(8127..8282)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) leader_d"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296231.1"
                     /locus_tag="E9931_RS08825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01729"
                     /product="lacticin 481 family lantibiotic"
                     /protein_id="WP_023612114.1"
                     /sec_met_domain="L_biotic_A (E-value: 6.5e-30, bitscore:
                     92.5, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="leader_d (E-value: 3.4e-08, bitscore:
                     23.1, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKNNEVINSIQEVSLEELDQIIGAGKNGVFKTISHECHLNTWAF
                     LATCCS"
     CDS_motif       complement(8208..8282)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MEKNNEVINSIQEVSLEELDQIIGA"
                     /locus_tag="E9931_RS08825_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(8127..8207)
                     /prepeptide="core"
                     /locus_tag="E9931_RS08825_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="5.20"
                     /molecular_weight="2778.2"
                     /monoisotopic_mass="2776.3"
                     /core_sequence="GKNGVFKTISHECHLNTWAFLATCCS"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEKNNEVINSIQEVSLEELDQIIGA"
                     /leader_location="[8207:8282](-)"
                     /alternative_weights="2796.2"
                     /alternative_weights="2814.2"
     misc_feature    complement(8435..8452)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 21.71"
                     /tool="antismash"
     gene            8480..9178
                     /locus_tag="E9931_RS08830"
                     /old_locus_tag="SAMEA3919035_01730"
     CDS             8480..9178
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294154.1"
                     /locus_tag="E9931_RS08830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01730"
                     /product="response regulator transcription factor"
                     /protein_id="WP_085613747.1"
                     /transl_table=11
                     /translation="MNFESQLLNKRILIVDDDVALSKSIKEVLISRDFKNISCAYSISE
                     GIDIFSVSKIDLIILDVMLPDGEGHLLAKYIRKYSDVPILFLTAKNNPDDEIRGLESGG
                     DDYVTKPFLPKSLTYRIIALLRRAYKDESELIELTNCTIDLNNASVEKNGQHLSLTPTE
                     IQILRKLFSNKNYIASTESICDTVWGLESLGYEKSLMVHIRNIREKIEITPSKPNHLIT
                     VKGLGYKLVI"
     gene            9196..10557
                     /locus_tag="E9931_RS08835"
                     /old_locus_tag="SAMEA3919035_01731"
     CDS             9196..10557
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098077.1"
                     /locus_tag="E9931_RS08835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01731"
                     /product="sensor histidine kinase KdpD"
                     /protein_id="WP_085613748.1"
                     /transl_table=11
                     /translation="MNIFKKNILKFILSFFFIIIIDVILLITATNYIRSQQSASTIIET
                     VSSEITLSSNGKYNVSSKAKQLIDDKKLWIMIIDKDNGKEKFNINKPQIIENQFDFADA
                     IRFSRFYLKDYPVFTQIKDNDIYIIAFPKDSIIRYSSNYFELNRVQIFPILILSIIFAN
                     CLFCLFLYIYSVTFLNRNIKPIINAITKLPNGINTQVKSVRELDKLTLAINSANQQLRE
                     NEEFKENWISGIAHDIKTPLSVIVSNTSLAIEKTNDDEFLKHLKPTLVESHYIQNLLND
                     LNIFARLTNGNLKLNHEIVDIIPFFKEIIIQIINQEIWEDFNFEFIPDSKLLGKKMYIE
                     KALISRVVHNLIYNSVLHNHSGCDIKIILEYLPEDCFSITIRDNGIGTSPERLKNINKI
                     EEFNFDMSGVRRSGMGLKISKQIIDLHDGNMIITSTQGQYFQTVITLPIESLNT"
     gene            complement(10773..11288)
                     /locus_tag="E9931_RS08840"
                     /old_locus_tag="SAMEA3919035_01732"
     CDS             complement(10773..11288)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022534.5"
                     /locus_tag="E9931_RS08840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01732"
                     /product="helical hairpin domain-containing protein"
                     /protein_id="WP_345769885.1"
                     /transl_table=11
                     /translation="MSFKTDCNTQSFVYFYSIHPLPYRKRVTLETLKEKVEKINLLMEI
                     HTPNQTFLETKDQLVQEIATLDMKIEEIQEKIATLNKMAEVFINLESDNKEGKRLARYN
                     LTKTNIPLTIKVDQVEREIEQLQISLTEHLDEYEGKVSELEEFVQMLNTLELPLTDRDH
                     LDRNRLAF"
ORIGIN
        1 tacaacccac atacggctaa tctaggacta acccctaatc agaaagaaga aaattagttt
       61 aacgtaataa aataacacgg ttttctgtct acttatttga cattagtcca catgctctca
      121 ccttttcctt ctataaattt agttacttct gtaatgtata aaaatccact ttatccattc
      181 ttctctaaag ttcgtaatgt caatttgaaa aaaataaaac tatatagagc attaacaact
      241 ataaatatta cgaactccga aatgctcata tctactaacg ctctactacg tatacctatc
      301 attggctggg aataagggaa aaattgatta atgacacctc ccaacataat aaatatgaat
      361 ccacaaaatg aacctatagc tgcaatcccg actgatttag aaaaattttt tgttttagcc
      421 atgatataac tatgaatcat tacaacagag ctagaaccaa taacagatag cacagttgct
      481 cttaaaaata aaagtaaatc ttttgctaca aacggaatct ggctcaattt cagtgttaca
      541 agatatataa caaataataa tacttgtata actgttataa gtagcaaagc taacaagaac
      601 ttactaagaa gcatcttttt aacagatact tgatttgcac gtaacatttc aaatgttttt
      661 cgctcaaatt caggtagaat aatcatgcct gagaatattg ctaacatagc tgggaaaagc
      721 aactgactat tatagaaagt taactgtccc cagagtactc tactcatttg ttcctcaatg
      781 agtacctcat aattcaaaaa gaaatttgcc aaaccaatga aactagatag aacaagaaat
      841 ataatcccaa ctgctaaaat agctcctttg cgagttttta gccactccgc tttgaaataa
      901 tcattcatta ttgtcccctt tcttcttttt cagtaaatac ttaactacgc tatagatcat
      961 tacgcagtaa ataaggtata aagtataatt taaaggcacg cttttgtccc aagaaaaaat
     1021 ataatctaaa ctatcactgg aatttaaaat ctttattcta tatagtgcta aaaaactcga
     1081 tcctccaaac ggaaaaataa ggttaatcca ctttaaagtc tgggaggtaa tcataccgaa
     1141 aaaacctgat aaaaatccaa ttcctaaaag aatcccttgt ttttctaaaa ttattgataa
     1201 ataaagaaag agagtgatta aagtaaaact ggacatcgtt aaacctacta cttgtaaaat
     1261 cagagtccaa attggaactt ggatatcata tcttagtaca tttagataaa caatcaagac
     1321 ttggaaaata ttcaaaagta gaaagacaga atttgtgaaa atcaatttgc gagtaaatat
     1381 tataattaga tttgtattat ttgcttcttg aagtttaaat gtactacctt ctttttcatt
     1441 accaacaata cgtgaaacaa ataggcaagt tgcaataggt aaaaaaatag aattagcttg
     1501 taaattatta aagaacaatc cgatagtccg taatttagga atatcaatac ttcttgaggc
     1561 tgctaacaat gaccacgtca ctgctactaa catcataatg aaagaaatca taaaagattt
     1621 cgtacgctta ctttttaata attcaatctg tattgctttt atcatttcaa acctccttag
     1681 ttaaagagag gaaaatgtct tctaaattct cgctttccct aacaactcgg aaaatccgat
     1741 agtcattttc aattaaataa gtcactacat ctgctaattt gtcattatca atatttttaa
     1801 acttaacatg acttgctgaa gctcctagca cttcaacctg tccgtaatta attaaacttt
     1861 gaacaatatt attttgcgag aaatccccgc caatctcaat ccaagtattt gattttattg
     1921 cttcaatttc cccttcataa accagtctcc catgatttat aatgcctact cggtcagcta
     1981 tatgctcaat ttcggataaa atatgacttg agataaatac tgttagccct ttctccttag
     2041 ctaattttac aattaactcg cgaatttcat gaattccagc cggatcaagt ccattagtag
     2101 gttcatctaa taacaagatt tttggttttt taaccagagc aaaagccaag gctaaccgtt
     2161 gcttcatgcc taaagagtag gctttcacaa gtttttttcg gttattttcc tctgctaaac
     2221 caaccaatgc caaagtcggc cagatattac tttcatcaaa acctaccatt ttttgaaaga
     2281 ctagtagatt ctcgtaacct gttaaatttg gataatagga gggttcctca atcattgaac
     2341 cgattacact caaataagcg tggttcccct ttatatcttc accatttata agaatttctc
     2401 ctagtgttgg agaaactagt gacaaaatca ttttcattgt tgttgttttt cctgcaccat
     2461 tcggtccaag aaaaccgtaa atctcgcctt cacgaaccgt aatcttttct aaatctaccg
     2521 tagtaatatc ctgatagacc ttagaaagtt ttcttaattc aatagcattt ttcatgttaa
     2581 taaccccata ataagaccct tcaatgagtc tcttcctttc cttctattac aacattattg
     2641 tatctaagca acttaaagat aacattaaga aaagagaaac attctcttaa tttttggaag
     2701 taatgaaatt tgtatagtta acatttgttt ccataagatg ttcatgagta tctttaataa
     2761 ccttaccagt ttcattatca ataaaaataa tagaatcaac ataattgaca atatctaaat
     2821 gatgactaac gattaacata gttctgtctg aatatctatt gttttgtaaa acattatcgt
     2881 aaattttgtt cttactttgt tcatctaaat tactgaaagc ctcgtcccaa aaaataattg
     2941 aattttcttt taaaagtgat tttgctatga gtaacttctg gatttgtcca cctgataaat
     3001 tttttccttg ttttaagagt ctagagtgaa tcttatttgg taatcgtgct ataacttcat
     3061 cagctgttgt atcattcaat acttggtaaa gtggagttac atcatctaaa gtccactcta
     3121 agttttctaa gacagtcccc tttctaggtg tcatattttg aagaacaact cttagattgt
     3181 ctttgaattt ttcgatgcca tattcaagag atccatcata gcgtagcata cctgctaaca
     3241 ttaatagtaa agttgacttc ccgctaccag atttacctac aatggcaatc ttttctcctt
     3301 tactgattgt taaattcaca tcactataga ttggtttatt accataagaa aagttcaaat
     3361 ttttagtttt gattaaataa tcgtctgact gtgaactagg tacaacgata ggtgaagtat
     3421 cttttaataa atcactttgt ttttctgcat atacacccaa tcgaattact tcaaatactg
     3481 ctccttttac ttgttcaatt gctgatatca ataaagaaat actagtttga taaactaata
     3541 gatcaggaag ggataaatta atataatcct tataatatac tgcgataaca attattgagg
     3601 atagactaat gtagttaaaa atagttccaa taagttgtga aagattttca attttaagaa
     3661 tctgcgaata cttcttagtg acttgattat tttgctccat ccaggaatgg aaaacagttt
     3721 tctctcttct cattattttt atatagtcaa tgtcatcaag atctgtttgt aactctgagt
     3781 taaaatcaat taagtactgt aaatatgtct gattatactc gttttgccta tgatacaata
     3841 gagtagagat tatactatat gaagcaatca tcaagattaa tatcaccgac aacttaatag
     3901 atatagtcat cagatatgca aagataataa tagctgaaat caaacttaca catgatggaa
     3961 taatttttaa agtaacattg tccctaagtg ttgatttaaa actaattttt tcagatactc
     4021 ccccacttag atgatttcta aaataaagta aaggtttttg taataatctt tgaaacaact
     4081 ctttactgta atatttatca aagtctgtat tatatctatc taatgcacca ttctttataa
     4141 aataacctaa taattgaaaa attaatactg cacctaaaag gaataagctg ataccaaact
     4201 tgaaatcttc agctaacatt tgtcttaaaa tgactgagaa cagtaaaaca ctaacttgaa
     4261 taaacaggag tgacaacaag aataatgcaa tcggttttaa ttgtaaggtc tgtttgaaat
     4321 acttccaaaa gattttctta tatgtttggg ggtgaaaatt atttcttttg ttaactgaca
     4381 ccatagtttc cgagtagtga gttaaaaatt catcacggct atacttaata cgaccaatgg
     4441 ctgggtcaac tataactgtt ttatttgacg taattttttc aagaaccaca aaatgatcat
     4501 tattccaatg aagaatcatt ctttgcttct tttctccgta tacttttagt aattcatcaa
     4561 aagaggcatg ataagcattt attttcaccc caaatctgtc acttagtgac aaaagatatg
     4621 agacatttag accatctgca ggcaatgtat ctttatcata aatttcatat aatggaactt
     4681 tatgtcctaa atcattaagt aacattgtat aacaagctaa gagacaatcc tcctcattgt
     4741 tttgcaaaat aatattcata tctcatattc ctttctatct cttagaaatt gggaatcaac
     4801 acatctaggg aatcatcacc cttcatgtac tttactgtag aaaataatat tcctgacaca
     4861 cctgtcatta acccataaac attatatttt gtattaaaac tacacatcca gccgttttcc
     4921 aggccaaaag aatgcagttt tctatactct ctatccaaaa tttcttttaa cttctctgaa
     4981 tcagctcctg ttaaatatga ttggtagtaa gtaagtgcca gcaaatttcc actggttcca
     5041 tggcaaagtg aaaagtttgt catatcaaaa ccaatttcaa gaatctgttt agcagcatgc
     5101 ttcatatcat tctctaattc tatccgttca gaatgagaaa gaaggctata ttcatcatct
     5161 agttttaact gtgctaacct tgcaactaat actcctgttg aaccatgaca ccagttggca
     5221 ctagtcgagg aatttctttt gtctatccag ccgttttcca atttttgatg tttatcaaac
     5281 atttttgctt tatgaaataa atttagtgcc tcaggacttt ctaactttcc agctaaataa
     5341 agtaacgcta tttcaactcc taaattacca tgtgcaagac taacattatt cgactctaac
     5401 ttatcccaac taataaaatc cttttgaaca attgccaact ctttaagttt gtctagtgcc
     5461 tgagtaagat tatctatctt aatatccgaa tttctcaaag cagtgatttc attaggaaaa
     5521 cttgatagaa aatcagcact taaaacatct acatccaatg tatatttgag attattataa
     5581 ttcatgatag aattttgaat ctcttgtcct gtaacaacac taattcgttt taaagctgag
     5641 taaattccca gcttccccac aaaaaaagaa tgatttggta atttcatata ggaattagat
     5701 aatacagaaa aaattcgttt tagacattct ttaacccttc tttgtcttcc tgagtctaga
     5761 aaatcataag cctctgataa agaaattgct attccagaca aaccttcata aagagagtca
     5821 tccatcggaa ctaactcaaa agcatcataa tctgtaactt tcaaggtgag ccagtttgta
     5881 ctatcgtctt ttagagagtt gctttcattt tccaaaataa tatcaactag agtatcaata
     5941 ccagttttta ttatattttc actgctacta accttctcaa atttttgata tgcctcttga
     6001 agttccgtac tatataaagc tttaggagtt ttgatagaaa actcaattaa ttcaatttgc
     6061 tcatttatga tttcttcact caatttttct aatttattga ttgcttcgct taaagcagaa
     6121 ctttcaaggt tccaaataat catgttttca tcctttatat cacaactatc aatatttgag
     6181 taaaaataag ggatatccat atttaaaagc tgtttctttt ctgatagaca taaactacga
     6241 ctattttgtt cagacaattt attggccatt gtttcaaata aaatctcgga ttgctcacaa
     6301 taaacaggag aaactagcaa ttggcgcact aagctataat ctcttgtatt tctgaacaaa
     6361 attctagtct ttatatcatt atgcttaata taaagcttct ttaaaaaatc ttgtgaattc
     6421 atgaaaaagt agctgacttc acggaaccct tctttgatat attcaacata atcttcagca
     6481 ttaagaaatt ctttcattcc cttcgaatca ataaagtaag gcaaatgatc ctggttttct
     6541 gttttaaact tttgtttttc aacatgaata tcatctcggt tatgattgat gacgattttt
     6601 gcctctccaa ttaaggtgcc tcctagaaca ccactagtat ctcctccaaa aactttacca
     6661 gcttcagaaa caggaagcaa acctgttgat aatatcgaat ttctactatt attaattatc
     6721 tccaatgttg actcgttatt tgcaacagtt tgataggggc taactgaaaa aactgtctca
     6781 acatcaacta acttaggaga aaagcttgta gaaatcagat tttcaaaatg caaatcagag
     6841 atattcaata tgtaagaata tgataataag taacccatcc tacgataaaa ttcctttgca
     6901 tcctcctcgg ttcttaacgg agaacttgat tcaaattttt cccaaaaata ctctccttta
     6961 tctaaaaaat cagggataaa gtctatatct ttctgtaaga atttaccaac ttctatattc
     7021 agttcatgca agagaacatt gggatttgat gattttttct taaaaactac tttttgttct
     7081 tcataactca aaatgcagac tccacaacca ttgtgaatat ctccagttat tttgattttg
     7141 acttcactaa tttttggagt ctgagcttca ctatagatat agcctgtctc tctcaactct
     7201 ttgaaatcta aagtaaatct ttcctttaca aaattaatta attccaaacg tttcttgata
     7261 gtactcatta cacgctgatt aatagaaggg aaacgtctat caatttcttc aaaaattaag
     7321 ccattctgac atagtacatt attaaagtat tcatagcgtg actctggaga atctccaatt
     7381 aggtttttct caattctttt ttcatttatt aggtaaataa gagtggaacg ataataatca
     7441 gaaatttcat cttcaacatc cttaatcagc tcactacttt catttagcaa ttctgggaaa
     7501 ttattttcaa taactacttt tgggaatcta tcaaattggc tatataacag ttcttttgga
     7561 ttcatattct ttactcctaa acatattaac aacaagtaaa aattgcctgt aaactattcc
     7621 aacgacattc agctgaaatt gtattgacac catgccctct tcctcccaaa aggttatcca
     7681 attcctcaat ctgaaattct tgtaacgcat tcaaagcacc taggcacatt ttttcttcca
     7741 taattcttta cctcatttat atgacatcga gtagaactcg aaatttgttt tctttataca
     7801 ttgattttaa atttgttaca taaagacaat attaaggaaa actaaagtta aattaaattt
     7861 tctacctatg tcaatgcaaa taaaaaaaga ggtccccctc tttttttatg taacatcaaa
     7921 gacacctgaa cagtagagtt ttatgaacaa caagtagcaa ggaatgccca tgtattcaaa
     7981 tgacactcat gagaaattgt cttaaataca ccgtttttcc cagcaccgat aatttggtcc
     8041 aactcctcaa gactaacttc ttgaatagag ttaataattt catgttcttt ggtcataacg
     8101 attttctcct tatattttat ctaatattat gagcaacaag tagcaaggaa tgcccatgta
     8161 ttcaaatgac actcatgaga aattgtttta aacacaccat tttttccagc accgataatt
     8221 tgatcgagtt cttcaagact aacttcttga atagagttga ttacttcatt atttttttcc
     8281 attttctata cctcatttaa ctttcttcag aaggataggt gccttcctct ttgttacaac
     8341 cttattttaa gatgataact ttaagacaga ataaagaaaa cataaattta attttaagat
     8401 tatacaacac aagtcctact tattctctta tatctataat ctttatggta taattttatt
     8461 acactttagg aggtattgta tgaattttga gtctcaatta ttgaataaac gcatattgat
     8521 tgtcgacgat gatgtcgcac ttagcaaatc aattaaagag gtacttatca gtcgggattt
     8581 caagaatatc agctgtgctt atagtataag cgaaggaatt gatatcttct cagtttctaa
     8641 aattgatctc attattttag atgttatgct accagatgga gaagggcact tacttgcaaa
     8701 atacattagg aaatattcag atgtgccaat tctcttttta acagccaaaa acaatcctga
     8761 tgatgaaatt cgaggacttg aatccggagg ggatgattat gttacgaagc catttcttcc
     8821 caaatcttta acatatcgta ttattgcact gttgagaagg gcttataaag atgaatcgga
     8881 actaattgag ctaacaaatt gtactataga cttaaacaat gcaagtgttg aaaaaaatgg
     8941 tcaacatttg tcactcactc caacagaaat acaaatccta cgaaaattat tttcaaataa
     9001 aaattatatt gcatcaactg aatcgatttg tgataccgtt tggggacttg agagccttgg
     9061 ctacgaaaaa tcacttatgg tacatattcg taatatcaga gaaaaaatcg agataactcc
     9121 atctaagcca aatcatctta ttactgtaaa agggcttggt tataaactag ttatataaac
     9181 tataaggaat atagcatgaa tattttcaag aaaaatatac taaaatttat cctatcattt
     9241 ttctttatta taataattga cgtcatactg ttaataactg ctaccaatta tatccgaagt
     9301 cagcagtctg catctactat aatcgaaact gtctcatctg aaatcactct atcttctaat
     9361 ggtaaatata atgttagttc taaagctaaa caactcattg atgataaaaa gttatggata
     9421 atgattattg acaaagataa tggaaaagaa aaatttaata ttaataaacc tcagattata
     9481 gaaaatcaat tcgattttgc agatgctata cgattttcta gattttacct aaaagactat
     9541 cctgtattta cgcaaataaa agataatgat atttacatca ttgcttttcc aaaagacagt
     9601 ataatacgtt atagtagtaa ttatttcgaa ctaaatagag ttcaaatttt tccaatcctt
     9661 attttgtcta tcatatttgc aaactgtttg ttctgcctat ttctttacat ttattctgtt
     9721 acttttttaa atcgtaacat taaacctatc attaacgcaa ttactaaatt accaaatgga
     9781 attaatactc aggtcaaatc tgtacgagaa ttagataaat taacacttgc tattaactca
     9841 gcaaatcagc aacttcgtga aaacgaagaa ttcaaggaga attggatttc tggaatagca
     9901 catgatataa agacaccact ttcagtaatt gtatctaaca cttccttagc cattgaaaaa
     9961 acaaatgatg acgaattcct taaacatttg aaacccacct tagttgaaag tcactacatt
    10021 caaaatctcc taaacgattt aaatatattt gctcgattaa caaacggaaa cttaaaacta
    10081 aaccatgaga ttgttgatat aatccctttc ttcaaagaaa taatcattca gattatcaat
    10141 caagaaattt gggaagactt caattttgaa ttcattcctg attctaaact attaggtaag
    10201 aaaatgtata ttgaaaaagc tctcatttct agagttgttc ataacctaat ttataattca
    10261 gtacttcata atcattctgg ttgcgacatt aaaatcattt tagaatatct acctgaagat
    10321 tgtttttcga ttactattcg tgataatggt ataggaactt ctcctgaaag actaaaaaat
    10381 atcaataaga ttgaagaatt taattttgat atgtcaggcg ttcgaaggag tggtatggga
    10441 ttgaaaattt ctaaacaaat tattgattta catgatggaa acatgataat tacaagtaca
    10501 caagggcagt acttccaaac agtaattacc ctccctattg aatcactaaa cacttaacaa
    10561 aatttttgat agagcagaga ttaaaatccg aaacataagt aaaaaataat gactttagct
    10621 ttctgttatt cagaatattt attttttagg ctctttcttc acgattgcaa atattattat
    10681 cggagataat atcaactatg aaaaaatttt taaaaatcat tatttagtat ttggtgtgat
    10741 agacactact gcaaataaat cactaagatc tattaaaaag caagtctatt cctatctaga
    10801 tgatctctat cagtaagagg aagttcaaga gtattaagca tttgaacaaa ttcctcaagt
    10861 tcactaacct tcccctcgta ttcatctaga tgttctgtta aagaaatttg taactgttct
    10921 atttctcgct ctacttgatc aactttaata gttaatggaa tattcgtctt agtcaggtta
    10981 tacctagcta gccttttacc ctctttatta tcactctcta gattgataaa cacttccgcc
    11041 atcttgttta aggtggcgat tttttcttgg atttcctcta tcttcatgtc tagtgtggct
    11101 atttcttgga ccagctggtc cttggtttca agaaaggttt ggttaggagt atgaatttcc
    11161 attagtagat ttatcttttc aaccttctct ttcaatgttt caagtgttac tcgttttcga
    11221 tatggaagtg gatgtatact ataaaagtag acaaaacttt gtgtgttaca atctgtttta
    11281 aaagacacaa aaacgaaagg acaacctatg tcaacagtta aacgctacga tgaagaat
//
