LOCUS       NZ_CAAHQE010000004     10228 bp    DNA     linear   CON 19-DEC-2024
DEFINITION  Streptococcus pyogenes strain Bel002, whole genome shotgun sequence.
ACCESSION   NZ_CAAHQE010000004
VERSION     NZ_CAAHQE010000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2019) WTSI, Pathogen Informatics, Wellcome Trust
            Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900985135.1-RS_2024_12_19
            Annotation Date                   :: 12/19/2024 10:09:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,745
            CDSs (total)                      :: 1,683
            Genes (coding)                    :: 1,623
            CDSs (with protein)               :: 1,623
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 28 of 60
            Pseudo Genes (incomplete)         :: 32 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 11 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:22:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 114344
            Orig. end                         :: 124572
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CAAHQE010000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10228
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10228
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10228
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5228](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5228
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            259..834
                     /locus_tag="E9931_RS05605"
                     /old_locus_tag="SAMEA3919035_01096"
     CDS             259..834
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /locus_tag="E9931_RS05605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01096"
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="WP_009880369.1"
                     /transl_table=11
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIVA
                     QETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFAAK
                     WHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPSPRN
                     QLWLAKNPWFEQDLLPILQKRVEAILTK"
     misc_feature    965..979
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 18.45"
                     /tool="antismash"
     gene            993..1490
                     /gene="ybeY"
                     /locus_tag="E9931_RS05610"
                     /old_locus_tag="SAMEA3919035_01097"
     CDS             993..1490
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ybeY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /locus_tag="E9931_RS05610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01097"
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="WP_002985748.1"
                     /transl_table=11
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVTN
                     ERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGELFI
                     SIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAYGLT
                     RQ"
     gene            1471..1878
                     /locus_tag="E9931_RS05615"
                     /old_locus_tag="SAMEA3919035_01098"
     CDS             1471..1878
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /locus_tag="E9931_RS05615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01098"
                     /product="diacylglycerol kinase family protein"
                     /protein_id="WP_002985746.1"
                     /transl_table=11
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLLS
                     ACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNAKD
                     MAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     misc_feature    complement(1930..1945)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.57"
                     /tool="antismash"
     gene            1998..2894
                     /gene="era"
                     /locus_tag="E9931_RS05620"
                     /old_locus_tag="SAMEA3919035_01099"
     CDS             1998..2894
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="era"
                     /gene_functions="other (smcogs) SMCOG1227: ribosome
                     biogenesis GTP-binding protein YsxC"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /locus_tag="E9931_RS05620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01099"
                     /product="GTPase Era"
                     /protein_id="WP_002985743.1"
                     /transl_table=11
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIMG
                     IYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDDMI
                     IERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTLIKL
                     LTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKRDEET
                     DKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVKVKKNW
                     RDKKLDLADFGYNEKEY"
     gene            2915..3391
                     /locus_tag="E9931_RS05625"
                     /old_locus_tag="SAMEA3919035_01100"
     CDS             2915..3391
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /locus_tag="E9931_RS05625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01100"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_002985741.1"
                     /transl_table=11
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKTW
                     AIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFIYE
                     LTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
     misc_feature    complement(2942..2958)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.42"
                     /tool="antismash"
     gene            complement(3698..3952)
                     /locus_tag="E9931_RS09145"
                     /old_locus_tag="SAMEA3919035_01101"
     CDS             complement(3698..3952)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990779.1"
                     /locus_tag="E9931_RS09145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01101"
                     /product="hypothetical protein"
                     /protein_id="WP_011106868.1"
                     /transl_table=11
                     /translation="MSGLFYHKSYFFKSEQVYDLLNEIRMRYPHAHIILIGNHINYEEI
                     FKNHYRVFGVIDTTSHKSLRSIRDQIQLYLDELYNSNNG"
     gene            complement(4076..4687)
                     /locus_tag="E9931_RS05635"
                     /old_locus_tag="SAMEA3919035_01103"
                     /pseudo=""
     CDS             complement(4076..4687)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /locus_tag="E9931_RS05635"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01103"
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTQFTTELLNFLAQKQDIDEFFRSSLEIAMNDLLQVELSAFLGYE
                     PYEKEGYNTGNSRNGTYSRQFETKYGLVNLIIPRDRNGEFSPVLLPSYARRDDHLEEMV
                     IKLYQTGVTTREISDIIERMYGHHLFLSGSLVTKRL"
     gene            5001..5228
                     /locus_tag="E9931_RS05640"
                     /old_locus_tag="SAMEA3919035_01104"
     CDS             5001..5228
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016754.1"
                     /locus_tag="E9931_RS05640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01104"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_002992018.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 8.5e-18,
                     bitscore: 58.2, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIKKLETFHQMTIEKLAKVEGGKNNWQANVSGVIAAGSAGAAIG
                     FPVCGVACGYIGAKTAITLWAGVTGATGGF"
     gene            5241..5441
                     /locus_tag="E9931_RS05645"
                     /old_locus_tag="SAMEA3919035_01105"
     CDS             5241..5441
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /locus_tag="E9931_RS05645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01105"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_003060803.1"
                     /transl_table=11
                     /translation="MTTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHPV
                     LTGGCVVGFTVGGAYLGYTAN"
     gene            5735..5929
                     /locus_tag="E9931_RS05655"
                     /old_locus_tag="SAMEA3919035_01106"
     CDS             5735..5929
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992021.1"
                     /locus_tag="E9931_RS05655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01106"
                     /product="bacteriocin"
                     /protein_id="WP_128888800.1"
                     /transl_table=11
                     /translation="MEVLKNGLAIINGGGKDGANIFLTGMAGAAEGVIVCAQTGVFIPW
                     QGYILCGAAGAATNIIWPH"
     gene            5979..6261
                     /locus_tag="E9931_RS09260"
                     /old_locus_tag="SAMEA3919035_01107"
                     /pseudo=""
     CDS             5979..6261
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528350.1"
                     /locus_tag="E9931_RS09260"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01107"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKILDIIFNLSDRKMIFLENVEKLGGYIVPFTLIILSVAYNFRHP
                     YPILGCCTRRYIPYCDLYLFYTYCLSIFAL"
     gene            6455..6865
                     /locus_tag="E9931_RS05665"
                     /old_locus_tag="SAMEA3919035_01108"
     CDS             6455..6865
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985720.1"
                     /locus_tag="E9931_RS05665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01108"
                     /product="hypothetical protein"
                     /protein_id="WP_002985720.1"
                     /transl_table=11
                     /translation="MRNILRFLGIVIISAVILFSIGSFYDLPLMKNIQLICWSFILDLF
                     VFVYKQRQTTEVLTWYQVVKQFWLFIKCTILIPTLIAFIIIIGCLTSISEVSIYFYLHL
                     TVVCYTIGMILSLGRLISPNHSIFNKLKKRNE"
     gene            7107..8177
                     /locus_tag="E9931_RS05670"
                     /old_locus_tag="SAMEA3919035_01109"
     CDS             7107..8177
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /locus_tag="E9931_RS05670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01109"
                     /product="hypothetical protein"
                     /protein_id="WP_136293853.1"
                     /transl_table=11
                     /translation="MMMLRDVKVKMSSLLVGCAATLLVSLSSTVAADSVHSSDRRFGVG
                     YKEETTSQFSSDQPSQVVLYRGEANTVRFTYTNQMSLMKDIRIALDGSGKSLTAQTVPG
                     MGHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMHNTMYKGTVFLV
                     QPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGGSGAFPDNIKVSIN
                     PKGGLATITYGAGSTDIIPPAVLWKKGSVKEPTEADQSVGTPTPGIPGKFKRDQSLNEH
                     EAMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNEDEKEKPASDVKVRPAEVGSWLEPA
                     TALPSVEMSAEDRLKS"
     gene            8270..8668
                     /locus_tag="E9931_RS05675"
                     /old_locus_tag="SAMEA3919035_01110"
     CDS             8270..8668
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /locus_tag="E9931_RS05675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01110"
                     /product="histidine kinase"
                     /protein_id="WP_194104144.1"
                     /transl_table=11
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLED
                     DAAILNHLEEDRNVNKFFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTGIT
                     YGDDTLADLAHHLLHSDGVIPSLNDKSA"
     gene            complement(9016..9285)
                     /gene="stcA"
                     /locus_tag="E9931_RS05680"
                     /old_locus_tag="SAMEA3919035_01111"
     CDS             complement(9016..9285)
                     /codon_start=1
                     /gene="stcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /locus_tag="E9931_RS05680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919035_01111"
                     /product="quorum-sensing system protein StcA"
                     /protein_id="WP_023605037.1"
                     /transl_table=11
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTAMTIGHSK
                     QNPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(9807..10049)
                     /locus_tag="E9931_RS09265"
     CDS             complement(9807..10049)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285423.1"
                     /locus_tag="E9931_RS09265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_030126784.1"
                     /transl_table=11
                     /translation="MKQQGHTVMMCLNKTLTIKLSAPLTSNDLVPLSTNEVIIMEPLLH
                     LLPKVVPVLIHLVDKANVSWLTMILQLPILLKTIW"
ORIGIN
        1 gctactcaga aattacaagg cattaaacag attgattttg tctatttctc tgctaaggat
       61 gttgtccgac atcctgttgt ggctgatatt atcaaggctt atgaaacgtc ctcagaggag
      121 atgaaggact tactaaaacc taaggctagt gacgttgaag ctgactacca gccaggcttg
      181 acaaaatacc ctgtgatcgg tcaagagcac taagaccttt acgatgatgt ttaatatggc
      241 ctttttaagg aggattctat ggatgattta accaaagcta ttatggcaga tgaggctaat
      301 ctctcctata ccgaaagagg tatttttcct ttatatgatg ctcctcaaac agctaggatt
      361 attattgttg gccaagctcc tggaatagta gctcaagaaa ccaaacttta ctggaatgat
      421 cgtagtggca tccgcttacg agactggcta ggtgtggaca atgacacctt ttaccactca
      481 ggattgtttg gcattatccc tatggatttc tattatcctg gtaagggaaa atccggtgac
      541 ttaccaccta gagaaggttt tgcggctaag tggcacccgc cattaagagc attgatgcca
      601 gaagttgaat tgacgatttt ggttggtcgc tatgctcagg acttttacct tggtaacaag
      661 gcttacaaaa ccttaactga aacagtccgt cactttgagg attacttgcc agactatttt
      721 ccacttgttc atccttctcc aagaaaccaa ttgtggttag ctaagaaccc ctggtttgag
      781 caggatttat tacctatctt gcaaaaacgt gtggaggcaa ttctaaccaa ataggccctg
      841 atttacctag cattcccatt tttaaggggc ttgtggtata atgggtaaat aacaaatgct
      901 gattgaaagg ataagggaaa ttatctgtca gataataatg ggtgcccaac ataccaaatg
      961 aggtgggcag cccttcccta acaattaacc ttatgtatat cgagatgatt gacgaaacgg
     1021 gacaagtttc gcaagagatt atggagcaaa cacttgattt gctcaatttt gctgctcaaa
     1081 agacgggcaa agaagaaaaa gaaatgtctg tgacctttgt gaccaatgaa agaagtcatg
     1141 aactcaactt agaataccga gatactgatc gtccaacaga tgtgatttct ctagaataca
     1201 agccagaaac gcctatttta tttagtcaag aggatttggc tgctgatcct agtttggcag
     1261 agatgatggc agagtttgat gcttatattg gcgaattgtt tatttctatt gacaaggcgc
     1321 gtgagcagtc ccaagaatat gggcactctt ttgagcgtga aatgggattt ttagctgttc
     1381 atggattctt acatattaat gggtatgatc actacaccct tgaagaagaa aaagagatgt
     1441 ttaccttaca ggaagagatt ttgactgctt atggccttac acgacaataa taccaccaaa
     1501 cggaaatgga aaaacagaac cattacctca agcttggagt ttgccttgac aggggttttc
     1561 acagctttta aggaagaacg caatctcaga agccaccttt tgtctgcctg tttagcttgt
     1621 gttgctggtt tattttttag catttcagcc atcgaatggt tatttttact tctagccatt
     1681 tttttggtca ttaccttaga aattgtcaat tctgctattg aaaatgtagt tgatttggct
     1741 agtgactatc acttttctat gctagctaaa aatgccaagg atatggcagc aggtgctgtt
     1801 ttgatgattt ctggctatgc tgtcttgaca ggacttatta tttttattcc taaaatttgg
     1861 aatattttcg ttcattaaaa agaaaggaat gggctatcct atgtgatgat ggcataggag
     1921 aataaggaaa ggacaagacg gttaagctta gtgacaagct tgatctgaag ccctaccgtt
     1981 aaggcccgta attatctatg tttaaatcag gctttgtagc cattcttggt cgtccaaatg
     2041 ttggcaaatc caccttttta aatcacgtca tggggcaaaa aattgccatc atgagtgata
     2101 aggctcagac cacccgtaat aaaattatgg gcatttacac cacagaaaca gaacaaattg
     2161 tctttattga tacgccaggt attcataaac caaagaccgc ccttggtgat tttatggttg
     2221 aatctgctta tagtacttta cgtgaagttg agacggttct tttcatggtt cctgccgatg
     2281 aaaagcgtgg taagggtgac gacatgatta tcgagcggct aaaagcagct aagattcctg
     2341 ttattttagt tatcaacaaa attgataagg ttcatccaga ccaacttttg gaacagattg
     2401 atgacttccg tagccaaatg gattttaaag aagtggtccc aatttctgcc ctagaaggta
     2461 ataatgtgcc aaccctaatc aagttattaa ctgataatct tgaagaaggt ttccaatatt
     2521 ttccagaaga tcagattact gatcatccag aacgtttcct tgtgtcagaa atggttcgtg
     2581 agaaggtact gcatttgacg caacaagagg ttccacattc cgtagctgtt gttgtcgaat
     2641 ccatgaagcg agatgaagag actgataaag tgcatatccg tgcaaccatt atggtggagc
     2701 gcgatagcca aaaaggcatc atcattggaa aacaaggagc catgctcaaa aaaatcggaa
     2761 aaatggcgcg ccgtgatatt gaactcatgc taggtgacaa ggtctactta gaaacgtggg
     2821 tcaaagtcaa gaaaaactgg cgggataaaa agcttgacct tgctgacttt ggttataatg
     2881 agaaagaata ctaacatagt aaagcgagca tcatatgcca caagactaca tttcttatat
     2941 ccgctccaaa gttgggcatg ataagattat tcttaacttt gctggtggga ttttgaccaa
     3001 tgatgacggc aaggtgctga tgcagttgcg tggtgataag aaaacctggg ctattcctgg
     3061 tggcactatg gaactgggtg agagctcttt agagacttgc aaacgtgagt tcttagagga
     3121 aaccggaatt gaggttgaag ctgttcgctt actgaatgtt tacactcatt ttgaggaagt
     3181 ttatcccaac ggtgatgctg ttcagactat tgtctttatc tatgaactaa cggctgtttc
     3241 tgatatggct attgataact ttcataatga ggaaacgctc aagttacagt ttttctctca
     3301 tgaggagata gcagagttag agagtgtctc agccaaacat cgcctgatgt tagaggaata
     3361 ttttagtgat agctttgcta tggggcatta agaactctct aggacagttg gagtattttt
     3421 ggagattggc ttgttaaaaa aatttttgaa agacgatgtt tcgagagctt atgctctctt
     3481 tttttgcttc caatttttct gattaagtct gaataatttt gttaaatgtt aacaggattt
     3541 tctttccaat tgatactagc agacatcaaa catttgtttt agagttatga cgaattgcca
     3601 cctagccatg catgacttag gcaagttgaa cctaactgtg gcaatagact tggaaggggg
     3661 tgacaagaat catttcttaa aagaattgat gaagtgccta tccattattc gaattgtaaa
     3721 gttcgtctaa gtaaagttga atttggtctc gaatggacct taatgattta tgacttgttg
     3781 tgtcaataac accaaaaact cgataatgat ttttaaatat ttcctcgtaa ttaatatgat
     3841 ttccgattag aataatatga gcatgaggat aacgcatacg tatttcgttt agaaggtcat
     3901 atacttgctc agatttaaaa aagtatgatt tatgataaaa taaacctgac atttgtattt
     3961 ataatctaag tttttcccac taataatttg agtttttaga aagtatctct tgaggtaata
     4021 gttaatatag atagatatca tatatcctaa aagagaacga tgtcttgtaa gtattttagt
     4081 cttcgaaaat acttacaaga catcgttcta aggcttcttc atttggaaaa accaccctcc
     4141 ttcttgcttt gtcgcttgat ttctttattg agtgattcaa tcaagtttgt agagtaaata
     4201 ctagaccaaa tgtaatgtgg aaattgatag aaagttagga gattttccgt tgtttcaagt
     4261 gatgtcataa cctttttgta acgaggcttc cactcagaaa caagtggtga ccatacatgc
     4321 gctcaataat gtcactgatt tcgcgtgtcg taacgccagt ctgatagagt ttgatcacca
     4381 tctcttccaa gtggtcatct cgtctagcat aagatggtaa taaaactggt gaaaactcgc
     4441 cgtttcgatc tcttgggatg attaaattga ctaagccata ctttgtttca aactgacgag
     4501 aataagtccc attacggcta ttacctgtgt tgtaaccttc tttttcataa ggttcataac
     4561 caagaaaagc tgatagttca acttgcaaga ggtcattcat agctatttct aaagaggagc
     4621 gaaagaattc atcaatatct tgtttttgtg ctaggaagtt aagtaattct gtggtaaact
     4681 gagtcatggg aataaatctc tttctagtaa gtgtcgtaac tctactatac aggatttatt
     4741 cctttttgtg tgtttacaca agatatttta cactaccatc aaaaatctta aaaggtattt
     4801 ataaaagcat gaatggtatt atttaaactt cttaattaac tattgtcaca gtgtaagtgt
     4861 agagaggata aaaagacatt tcatgatgaa aaaacagcct tttaagattt tgaaaccatg
     4921 atactgttct ttatgatatt ctgtactagt cgaggttgca acaagacaaa tttaagaatt
     4981 atgaaaagaa gttattcgat atggagataa agaaactgga aacatttcac caaatgacta
     5041 tagaaaagct tgccaaggtt gagggtggta agaataattg gcaggcaaat gtcagtgggg
     5101 ttatcgctgc aggtagtgct ggagctgcta taggttttcc agtttgcggt gtagcctgtg
     5161 gttatattgg agctaaaaca gctattactc tttgggctgg tgtaacagga gctacaggcg
     5221 gattctagag gaaagaaagg atgacaacaa tgaaggaatt aacaattaat gacatggcta
     5281 gtataagtgg aggaaacgct ccaggagatg ctgtaattgg tggtcttgga ggcttagctt
     5341 ctggattgaa attttgtaaa ctgccacatc cagtattgac aggtggatgt gttgtaggat
     5401 ttacagttgg tggagcatat ttgggatata cagcaaatta aatgatctat acgattttta
     5461 ttattatgaa tgcgtctata atgtttttaa tcattgttga tgccattcat aaaaaaatgt
     5521 tgataaatct ataccatatt tagtgttttt aattttcaat caattatccc tttcagaaaa
     5581 atcggtaaac atgttatcta tcgtagcttt ggtagcgtca ttatttgttt ttatcgataa
     5641 aagaaaatag tgattatgcc aatgaattta gatatcactc ttagcttttt aaaatacaaa
     5701 agagtgaaat taaaatgaaa aagacttcaa aaatatggaa gtgctaaaaa atggtctggc
     5761 tatcataaat ggaggcggaa aagacggagc aaatatcttt ttgacaggga tggcaggtgc
     5821 tgcggaagga gtgatagttt gtgctcagac aggcgtattt atcccttggc aaggttatat
     5881 tttgtgtgga gcagctggtg ctgctacaaa tattatctgg cctcactaaa tatataaatg
     5941 actatctatc actgatgtat aagtagtaga ggagtggtat gaaaatttta gatattattt
     6001 ttaacctatc tgatagaaag atgatatttc tagaaaatgt tgaaaagctg ggaggttaca
     6061 ttgtcccttt tacccttatc atcttgagtg tagcctacaa ctttaggcac ccttatccta
     6121 ttttgggctg ctgtactagg aggtatatcc cttattgtga tctttattta ttttatactt
     6181 attgtttatc tatatttgcg ttatagatac ttggtgtgga aaaaaatacc agaaattaag
     6241 attaatttta ctgagctata atgcaatcac atttaagggg aaaatggatg acttatacaa
     6301 tatcagtact tatgatattt aactttagag actaaagagt taaaagcaaa atgcgtattg
     6361 aaaattggtt aaaaaagtta taactgtctc tgataacagg aataacaatt tgttcggtta
     6421 tatttgctac cgatagagta gaggaggtga agatatgcgt aatatattaa ggtttttagg
     6481 tattgttatt atttctgcag tgattctatt tagtataggt agtttctacg atttaccatt
     6541 aatgaaaaat atacaactta tctgttggag ttttatcctt gatttattcg tttttgtcta
     6601 caaacaaaga caaacgacag aagtgctcac atggtatcaa gttgttaagc aattttggtt
     6661 atttattaag tgtacaattc ttattcctac cttaattgct tttattataa taataggatg
     6721 tttgacgagt atatcagagg tatcaattta cttttattta caccttactg ttgtttgcta
     6781 tacaatagga atgattttat cattaggtcg tcttatttct cctaatcact ctatatttaa
     6841 taaacttaaa aagcgaaatg aataatattt aaagtttgtt tttaaataat gggactagtt
     6901 tagtgtaacc gattcaatta aagaatagtt cgtgacaaga agataccttt gaagtttcag
     6961 aaacaatctg ttttccatcc tgctatactc atctttgcca aagctcgtca ttttatatga
     7021 tttatataga attacggttt ttttgttaga ctaatatgag aaaatatagt atgacgaatt
     7081 cctaaataaa aaataatgaa aggaacatga tgatgttaag agatgttaaa gttaaaatga
     7141 gcagtttatt agttggttgt gcggctactt tactagtcag tttgagtagt acagttgcgg
     7201 cagattcagt ccattcgtct gatagacgtt ttggagttgg ctataaggaa gaaacaacct
     7261 ctcagttttc ttcggatcag ccctcccaag tggttttgta tcgaggtgag gctaacacgg
     7321 ttaggtttac ctataccaat cagatgtctc tgatgaaaga tattcgcatt gctttggatg
     7381 gttctggtaa gtctttgacc gctcagactg ttcctggtat gggtcatgtt tatgagggct
     7441 ttcaaacttc tgctagaggg atttttacga tgtcaggagt tcctgaaagc actgttcccg
     7501 ttgctaaccc taatgtacaa accaaatata caaggtattt caaagtcatt gatgatatgc
     7561 ataacacaat gtataaagga actgtttttc ttgttcaacc gcaagcttgg aaatacacca
     7621 tgaaatctgt tgatcagtta ccagtagatg acttgaacca tattggcgtt gctggtattg
     7681 aacgaatgac aactctcatt aaaaatgcgg gtgccctttt aaccacagga ggtagtgggg
     7741 ctttcccgga caatattaaa gtatctatta atccaaaggg ggggctggcc acgattactt
     7801 atggggctgg ctctacggat attattcctc cagcagtttt atggaaaaaa ggctccgtaa
     7861 aagagcctac tgaagccgat caatctgtcg gaacaccgac tcctggtatt cctggtaaat
     7921 tcaaacgaga ccagagcctt aacgagcatg aagctatggt aaatgtcgaa ccactgtctc
     7981 atgtagtaaa agacaatata aaggtcatag atgaaaaatc aacagggcgg tttgagcctt
     8041 ttagacctaa tgaagatgag aaggagaagc ctgccagcga tgttaaggta agaccagcag
     8101 aagttggtag ctggctagaa ccagcgacag ctcttcctag tgttgaaatg agcgctgagg
     8161 acaggttaaa aagttagtta ataatttgcc ggttgcttta gacaggcaaa caggaaaggg
     8221 cttggaggtt tcttctgaag tagctgtgag cgcctcacag ccggaagaat tgcatcgatt
     8281 gcctaaccgt ttgatacctg aggaagacaa taataaacta tcacgagtag ccaaggaagt
     8341 cgttactgag ccaaggctaa ataacccctt tttgttaccc aagaaggttc tttcgttaga
     8401 agatgatgct gctatcctta atcatttgga ggaagaccgt aatgtcaata agtttttcct
     8461 cgagccagtt gcggctgaag ccaagagaga aattaatttt catccaagtg accagcaggt
     8521 gactgctaaa gttaaggagt catctgattt tcatcagaaa cagcaccaaa cagggattac
     8581 ttatggagat gatactctgg cagatctggc gcatcatttg cttcattcag atggtgttat
     8641 tcctagtcta aatgataaga gtgcttaaga attatgtgct agtaagtctc gctaagaaga
     8701 ggagtgttac aagataaata aagaagaggt cagggaacta ccggcctctt tttgtccgaa
     8761 atagcgagaa tgactctagc ttacctgtat gtaggtccgt gctacacatc ttgttaatga
     8821 tagggagctg ggatcagaaa ataattggta tatcgttttc tcctaacttt ttaagaaagt
     8881 gcgggcaaag tgggctgtta gggtgatggg gttgctcttt catgatcagt tttttagctt
     8941 gaagaaagcg tggtgatgag attagcttaa aaaagaacac taccgaaatg gtagtgtcca
     9001 ctgattttag gagagctaag attggtcatc taacaggagt ctaactgtca tgctaataac
     9061 agatttgata gcgctgtccc cacttggaag aaatagagcc ttatttttat tttttttgtg
     9121 tttaagggct tcttttttgt gagggttttg ttttgagtgg cctatggtca tcgcagtcct
     9181 tttcctttca atagtgtgtt taggaattgg tggtttaggg ctgacggcaa gaataaaaca
     9241 cagtatgcta atagctaaga taagataaaa ctgttttttg gtcattgttt aggcctttct
     9301 agttgaaaaa tgtagttgtt gttagtttag cctttttggt agctggtatt taaaatagta
     9361 ttagccaagt ggttgtactg gctatctaaa cggtactcag tattcctcac gtcttttcca
     9421 gcaaggaaat accttgtggt gtatgctgaa tcattccagg tagcatcaac gtaggaatta
     9481 acaccatgaa tggtaacgat gtttcaagta tggtgctcat tcaatccttt tttatcacat
     9541 accaagttga taagcctgca gcttctgcca tatctttaaa catgacggca taagcttgac
     9601 aagcaccaca cttgccacga taaagagctt taggagtatg attgttgatg ctgcttacgg
     9661 ttttgcgatt ggtgctaagt tattttgtgg cgtaagtgta ttcgttgatt aaatggttat
     9721 aaataacctt agccttgtcg tagtcactgg tgatcggttt atctgtaata tttttttgac
     9781 aaaggtttta tattccttat gaagggctac caaattgtct ttaagagtat aggtaactgt
     9841 aatatcattg ttaaccatga tacgttggct ttgtctacca agtgtataag tactggtacg
     9901 actttgggaa gtaaatgaag taagggttcc ataataatca cctcattcgt cgagagtggc
     9961 accaggtcgt tgctggttaa tggagctgat agtttgatag taagtgtttt gtttagacac
    10021 atcataacgg tatgcccctg ctgtttcatc agctttgacc ttgctgtcaa tggcagcaaa
    10081 cgcacttaag agtaaagctg ccgctaggtc gctaacactc tttttaatat ctattagttt
    10141 tcctgttaca aactaaatat aagggtttcc caaaagtgga ttgtttttaa gcgaaaggtt
    10201 atccaccaac gataatcagg atatccat
//
