LOCUS       NZ_CAAHMR010000004     20873 bp    DNA     linear   CON 11-MAY-2024
DEFINITION  Streptococcus pyogenes strain Bra016, whole genome shotgun sequence.
ACCESSION   NZ_CAAHMR010000004
VERSION     NZ_CAAHMR010000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2019) WTSI, Pathogen Informatics, Wellcome Trust
            Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900984225.1-RS_2024_05_11
            Annotation Date                   :: 05/11/2024 02:59:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,704
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 22 of 43
            Pseudo Genes (incomplete)         :: 20 of 43
            Pseudo Genes (internal stop)      :: 16 of 43
            Pseudo Genes (multiple problems)  :: 13 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:18:04
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 84533
            Orig. end                         :: 105406
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CAAHMR010000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20873
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="1"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20873
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20873
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10873](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10873
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    complement(764..784)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 25.95"
                     /tool="antismash"
     misc_feature    1658..1671
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.93"
                     /tool="antismash"
     gene            2045..2275
                     /locus_tag="E9727_RS07075"
                     /old_locus_tag="SAMEA3919085_01397"
     CDS             2045..2275
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105222.1"
                     /locus_tag="E9727_RS07075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01397"
                     /product="YkuJ family protein"
                     /protein_id="WP_002983885.1"
                     /transl_table=11
                     /translation="MESHLVRIINRLELMVTDGGNLKRNFERDGVVVAEVAFSNDPENG
                     PVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            2403..3089
                     /locus_tag="E9727_RS07080"
                     /old_locus_tag="SAMEA3919085_01398"
     CDS             2403..3089
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018000.1"
                     /locus_tag="E9727_RS07080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01398"
                     /product="amino acid ABC transporter permease"
                     /protein_id="WP_032464463.1"
                     /transl_table=11
                     /translation="MDLSFLPKYWAYFNYGVLVTIMISISVVFFGTLIGVLVTLIKRSH
                     VKPLTWVVNLYVWIFRGTPMVVQIMIAFAWMHFNNMPTIGFGVLDLDFSRLLPGIIIIS
                     LNSGAYISEIVRAGIEAVPKGQLEAAYSLGIRPQNAMRYVILPQAFKNILPALGNEFIT
                     IIKDSALLQTIGVMELWNGAQSVVTATYSPISPLLVAAFYYLMVTTVMAQLLAVLERHM
                     SQGGNH"
     gene            3089..3823
                     /locus_tag="E9727_RS07085"
                     /old_locus_tag="SAMEA3919085_01399"
     CDS             3089..3823
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407690.1"
                     /locus_tag="E9727_RS07085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01399"
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="WP_002989107.1"
                     /transl_table=11
                     /translation="MTEAIISIKDLHKYYGHNEVLKGIDLDIMPGEVVVIIGPSGSGKS
                     TLLRTMNLLEVPTKGQIRFEGIDITDKKNDIFSMREKMGMVFQQFNLFPNMTILENITL
                     SPIKTKGMAKAEADKTALSLLDKVGLSEKAKAYPASLSGGQQQRIAIARGLAMDPDVLL
                     FDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAKEVADRVIFMDGGVIVEEG
                     SPNQLFDLTKEERTKDFLSRVL"
     gene            3972..5381
                     /locus_tag="E9727_RS07090"
                     /old_locus_tag="SAMEA3919085_01400"
     CDS             3972..5381
                     /EC_number="4.1.99.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989109.1"
                     /locus_tag="E9727_RS07090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01400"
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="WP_134984640.1"
                     /transl_table=11
                     /translation="MVSVMWFRRDLRIDDQKALAHAIASQSPTLCVFQFNKEQLNLLHS
                     RNQSSFITSVLAFRKLLKCEGIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGYGR
                     LRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKETPIK
                     VELSQGRWLNLETPDDVLRTVESIKDEKYQDVATFDEASKQLNRFIQDQLAAYHANRDF
                     PAQLGTSRLSPFLRIGAIGIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYVAYPDQ
                     KTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRMIVASFL
                     TKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVTQGKRFDP
                     KGEFIKAYLPQLEHVPEKYLHEPWKMPKNLQESVSCIIGTDYAQPIVDHAKQREQAIAK
                     YEWAKEKAKIE"
     misc_feature    complement(5497..5508)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.16"
                     /tool="antismash"
     misc_feature    5526..5541
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 22.0"
                     /tool="antismash"
     gene            5559..7253
                     /locus_tag="E9727_RS07095"
                     /old_locus_tag="SAMEA3919085_01401"
     CDS             5559..7253
                     /EC_number="5.4.2.-"
                     /GO_function="GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /locus_tag="E9727_RS07095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01401"
                     /product="phospho-sugar mutase"
                     /protein_id="WP_002993334.1"
                     /transl_table=11
                     /translation="MTYNEVYQEWLHNNDLSDDIKADLAAIKDNEAEIQDRFYKTLEFG
                     TAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAVKKGIAVSYDVRYQSRTFAEL
                     TCSIMAANGIKAYLYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQEGS
                     QILDDIADQIAQHMAALTQYEEIKQMPFEKALDSGLVTYIDESIEEAYKKEVLGLTIND
                     TDIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPNPEVPK
                     TFAYSEKLGKAVDADILIATDPDCDRVALEVKNAVGDYVFLNGNKIGALLSYYIFSQRF
                     DLGNLPANPVLVKSIVTGDLSRAIASHYGIETVETLTGFKNICGKANEYDVTKQKNYLF
                     GYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKKKGQNLLDVLQTIYATFGYYNERQI
                     ALELEGIEGQKRIARIMEDFRQTPIASVAEMALDKTIDFIDGYQDFPKQNCLKFYLDDG
                     SWYALRPSGTEPKIKFYLYTIGQTQENSATKLDAIEAACRTKINQVN"
     misc_feature    7423..7439
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 23.75"
                     /tool="antismash"
     gene            7461..8315
                     /locus_tag="E9727_RS07100"
                     /old_locus_tag="SAMEA3919085_01402"
     CDS             7461..8315
                     /GO_function="GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /locus_tag="E9727_RS07100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01402"
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="WP_002989114.1"
                     /transl_table=11
                     /translation="MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDPA
                     SQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPNHI
                     NDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKHAVI
                     IGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQYIKE
                     GAIVIDVGMNRDDNGKLIGDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQSALRS
                     THK"
     misc_feature    complement(8382..8398)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.76"
                     /tool="antismash"
     misc_feature    8391..8405
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.26"
                     /tool="antismash"
     gene            8468..9808
                     /gene="xseA"
                     /locus_tag="E9727_RS07105"
                     /old_locus_tag="SAMEA3919085_01403"
     CDS             8468..9808
                     /EC_number="3.1.11.6"
                     /GO_component="GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA]"
                     /GO_function="GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /GO_process="GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="xseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /locus_tag="E9727_RS07105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01403"
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="WP_002992349.1"
                     /transl_table=11
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQYF
                     SLKDESAVIQATMWAGVYKKLGFDLEEGMKINVIGRVQLYEPSGSYSIVIEKAEPDGIG
                     ALALQFEQLKKKLTAEGYFEQKHKQPLPQFVSKIGVITSPSGAVIRDIITTVSRRFPGV
                     EILLFPTKVQGDGAAQEVVANIRRANQREDLDLLIVGRGGGSIEDLWAFNEEIVVQAIF
                     ESQLPVISSVGHETDTTLADFVADRRAATPTAAAELATPITKTDLMSWIVERQNRSYQA
                     CLRRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSMTLMNTMKDRLSSAKENKVQ
                     LDHALANSQLQTKIERYQDRVATAKRLLMANMASQYDSQLARFEKAQDALLSLDASRII
                     ARGYAMIEKNQALVASVSQITKGDQLTIKMRDGQLDVEVKDVKNENI"
     gene            9786..10001
                     /locus_tag="E9727_RS07110"
                     /old_locus_tag="SAMEA3919085_01404"
     CDS             9786..10001
                     /EC_number="3.1.11.6"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /locus_tag="E9727_RS07110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01404"
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="WP_002983901.1"
                     /transl_table=11
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKEL
                     QKTLQAAEKTLVKVMQADGTEVDMDD"
     gene            10001..10873
                     /locus_tag="E9727_RS07115"
                     /old_locus_tag="SAMEA3919085_01405"
     CDS             10001..10873
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /locus_tag="E9727_RS07115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01405"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_011054706.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 4.6e-52, bitscore:
                     170.2, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKLARIDEAIRRYYKTTSNGVSEELIDAILYSVDSGGKRIRPLI
                     LLEMIEGFGVSLQNAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFGEAT
                     AILAGDSLFLDPFGLIAQAELNSEVKVALIQELSLASGTFGMVGGQMLDMKGENQALSL
                     PQLSLIHLNKTGKLLTFPFKAAALVTDQAITVRQQLEQAGMLIGHAFQIRDDILDVTAS
                     FEDLGKTPKKDLFAEKATYPSLLGLEASYQLLTESLDQALTIFQTLENDVGFKPQIITK
                     LIEGLRLNA"
     gene            10866..11693
                     /locus_tag="E9727_RS07120"
                     /old_locus_tag="SAMEA3919085_01406"
     CDS             10866..11693
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /locus_tag="E9727_RS07120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01406"
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="WP_046176944.1"
                     /transl_table=11
                     /translation="MPKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPGD
                     KIDDGTELKLKGEKLKYVSRGGLKLEKGLHVFGVSVANQIGIDIGASTGGFTDVMLQDG
                     AKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFDEGQPVFASIDVSFISLSL
                     ILPALHNVLSDQGQVIALIKPQFEAGREQIGKKGIVKDKQIHEKVIQKVMDFASGYGFT
                     VKGLDFSPIQGGHGNIEFLAHLAKSQTPETLAPHLIQKVVAKAHKEFEKHEKE"
     gene            11680..12150
                     /locus_tag="E9727_RS07125"
                     /old_locus_tag="SAMEA3919085_01407"
     CDS             11680..12150
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /locus_tag="E9727_RS07125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01407"
                     /product="arginine repressor"
                     /protein_id="WP_003056949.1"
                     /transl_table=11
                     /translation="MKKSERLELIKKMVLTHPIETQHDLLRLLAEHGLELTQATISRDM
                     NEIGIVKIPSGSGRYIYGLSQDSRKKIIQGPRSIKSTILAISDPTAGLEQHLYLKVVPG
                     NSKLIKRYLLTDFPEAIFSLIADDDSLLLIAKSAADADKIRQEVLLWMQGQT"
     gene            12169..13830
                     /gene="recN"
                     /locus_tag="E9727_RS07130"
                     /old_locus_tag="SAMEA3919085_01408"
     CDS             12169..13830
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /locus_tag="E9727_RS07130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01408"
                     /product="DNA repair protein RecN"
                     /protein_id="WP_134984639.1"
                     /transl_table=11
                     /translation="MLLEISIKNFAIIEEISLNFENGMTVLTGETGAGKSIIIDAMNMM
                     LGARASTEVIRRGANKAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGRSV
                     SRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKENYQL
                     IFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDRLMNH
                     KQIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYYILEEV
                     SKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNVNDVLDYYDNIIKEYQLLTGD
                     DLSSGDLESELKALEKQLVAAATNLSERRHQLAEQLEAEIKAELKELYMEKADFKVHFT
                     TSKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKEDKTSIVFD
                     EVDTGVSGRVAQAIAQKIYKIGRHGQVLAISHLPQVIAIADYQYFISKESKEESTVSKV
                     RLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH"
     gene            14002..14931
                     /locus_tag="E9727_RS07135"
                     /old_locus_tag="SAMEA3919085_01409"
     CDS             14002..14931
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285633.1"
                     /locus_tag="E9727_RS07135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01409"
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_002989123.1"
                     /transl_table=11
                     /translation="MTSKKACLSSIIVLASLTCGNDTVSANHLSATGDKFDDCSTLVEK
                     DVAPKDELEMLAWSSSQTTDDADRDYEDFLDDDSFNDDSFISQNETDKMFENLTDDRLL
                     DELDEEDTIEPEQNVIMPSDDELFDLTDAVETRLTVSSAPHLEAELPKPHLRSLSDTAL
                     RSGEIRGHLDNKLDALSVTATKLALTMAQKFDLTTHVYSIGESFSEVLAAHYEDRKAES
                     AFSKKKRFHLPIATPDVVIEELRRLVSSIGSSKEDVSVPYNRKLGMAVAKRKIALPQTG
                     EEFSYYPALLGLMILGLTPIMIPKKINN"
     gene            15159..15998
                     /locus_tag="E9727_RS07140"
                     /old_locus_tag="SAMEA3919085_01410"
     CDS             15159..15998
                     /GO_function="GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264715.1"
                     /locus_tag="E9727_RS07140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01410"
                     /product="DegV family protein"
                     /protein_id="WP_002989125.1"
                     /transl_table=11
                     /translation="MGTIKIVTDSSITIEPELIKALDITVVPLSVMIDSKLYSDNDLKE
                     EGHFLSLMKASKSLPKTSQPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTIEASRQG
                     AEIAEAPVTVLDSGFTDQAMKFQVVEAAKMAKAGASLNEILAAVQAIKSKTELYIGVST
                     LENLVKGGRIGRVTGVLSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLDSYLAKNSH
                     RPIAEIAISYAGEASLALTLKERIAAYYNHSISVLETGSIIQTHTGEGAFAVMVRYE"
     gene            15991..16833
                     /locus_tag="E9727_RS07145"
                     /old_locus_tag="SAMEA3919085_01411"
     CDS             15991..16833
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /locus_tag="E9727_RS07145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01411"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_011888746.1"
                     /transl_table=11
                     /translation="MNNRHLFSGIFFFVISLCLAFLLLNIIIPKSNSRLKKSDFLKKEQ
                     VAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQILDR
                     MIKQKQIQLSLKKADIMTLTVGGNDVMAVIRKNLADLQVSSFRKPARQYQKRLRQIIEL
                     ARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNIKTKEVVGEYDRVYFVPINDLL
                     YKGINGQEGIVHSSGDQTTIVNDALFTGDHFHPNNTGYQIMSNAVMEKIKKHEKKIKP"
     gene            16811..17398
                     /locus_tag="E9727_RS07150"
                     /old_locus_tag="SAMEA3919085_01412"
     CDS             16811..17398
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /locus_tag="E9727_RS07150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01412"
                     /product="YpmS family protein"
                     /protein_id="WP_002989129.1"
                     /transl_table=11
                     /translation="MKKKSNLNWWKWSFLCLLAFNTAFLMVIASRLIQVREPESELIAK
                     KPVKNIKIGTFVTTREQLNETVASYLKDYQTKKMSYKFYATSSSILFEGTYQLLGYEVP
                     LYIYFQPHRLENGAVQLQVISFSVGTLPLPEKDVLQYLKSSYKLPSFVKVMPNQSAIVV
                     NLQDIQNDAKVYLKAKKIDLFNDEISFNIYKK"
     gene            17497..17772
                     /locus_tag="E9727_RS07155"
                     /old_locus_tag="SAMEA3919085_01413"
     CDS             17497..17772
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1132: DNA-binding
                     protein HU 1"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /locus_tag="E9727_RS07155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01413"
                     /product="HU family DNA-binding protein"
                     /protein_id="WP_002983920.1"
                     /transl_table=11
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSTIEAFLAEGEKVQL
                     IGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     misc_feature    18053..18066
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 26.23"
                     /tool="antismash"
     misc_feature    complement(18111..18125)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.54"
                     /tool="antismash"
     gene            18116..19978
                     /locus_tag="E9727_RS07160"
                     /old_locus_tag="SAMEA3919085_01414"
     CDS             18116..19978
                     /EC_number="7.2.2.-"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0015662 - P-type ion transporter activity
                     [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1037: heavy metal
                     translocating P-type ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /locus_tag="E9727_RS07160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_01414"
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="WP_109828819.1"
                     /transl_table=11
                     /translation="MTIRQWMADHLHLMETLACLVLIIIGLAFLHSFPQVASAIFITAF
                     LIGGYASAKTGILDLVKNKHLSVDILMILAAIGAGIIGYWLEGALLIFIFSLSNTLEEM
                     AMEKSKDAISGLMSLTPDTARQYQEDGHILEVETRSLSVGDRLQVRKGEAVPIDGQLLS
                     PFGQFDESMVTGEPITVDKAEGQDLIGGTINQGQTIDMLVTIENDDTLFAKIINLVESA
                     QEKKSKTATFIESLEDGYVKFVLVLIPAFILFSHFVLSWTWLAAFYRGMILLTVASPCA
                     LIASSTPASLAAISRAARKGLIIKGGDIVDNMGDIKAVVMDKTGTLTQGKPSVVNAHYL
                     EDELLVNRLVKGAEAASTHPISKAFLEYTEKLEPLTFDHLEEISGKGFQGFYQGQEWRI
                     GKKSFILEMVPDLSAFEETIQVEESQGKTLIFVSRDNQLVAYYALLDDIKLESKRAIEA
                     LHAMGIKTVMLTGDQERTANYVAQKLGIDEVVANCMPQDKVAKLAELKTKYGFVAMVGD
                     GINDAPALAQADVSYAIGSGTDIAMESADSVIMDDLTRIPFSIQLSRKMKTIIKQNIVF
                     ALSVITLLILANVFQVVNLPLGVVGHEGSTILVILNGLRLLSFK"
     misc_feature    complement(20010..20023)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.77"
                     /tool="antismash"
     misc_feature    20189..20201
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 22.13"
                     /tool="antismash"
     misc_feature    20254..20269
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.81"
                     /tool="antismash"
     misc_feature    complement(20286..20300)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.55"
                     /tool="antismash"
ORIGIN
        1 gaacttgtaa gaacatgtgc atgacgtcag gatgggcttt ttccacctca tctaagagaa
       61 taagtgaata tggattgcga cgaacttgtt cggttagctg tccagcttct tcatagccga
      121 cataacctgg aggagccccg actaatttgg cgacagcgtg tttttccatg tattcggaca
      181 tgtcaaagcg aatcatattg tttgtcgaac caaagagttc aatggctagt tgtttagaca
      241 attcagtttt accaacccca gtcggtccaa cgaataagaa agaaccaata gggcgatttg
      301 gagttcctaa tcccacacgg ttacgacgaa tagccttagc gattttatca acagcatcgt
      361 cttgcccgat cacgtgtgct ttcagatcat tggctaagtt tacgagttga gactgttcct
      421 tttcttttaa gtccccaact ggaatattag ttttttgctc tacaatagct tcaatggttt
      481 tttcagtaat gatgggaata tcttgctcat cgactttttg agcctgcatt tctttgtatt
      541 ttgtaatttg atcgcggaaa taagctgcgc gttcatagtc ttcgtctcga gtagcttgcg
      601 ccttgagatt ctcagcttca ataagacgtt tgtcaatttc tttaggatca acaaagttga
      661 gggttagatt cattttggaa ccagcttcgt ccagaagatc aatagcctta tcaggaagaa
      721 aacggtcttg aatatagcgg ttagataaat gagcggcagc ttcaatagcg gctgggctat
      781 atttgacatg atgatagtct tcgtatttcg gttggatacc ttttaaaatc gtgatggttt
      841 cctcaacaga aggttcatca actttaacgg gttgcatacg tcgttctaag gcagcgtctt
      901 tttcaataat acggtattca tttaatgtag tagcaccaac gagttgcaac tcaccacggg
      961 ccaaggctgg ttttaaaata ttaccagcat ccatattgcc gtctcctgca gaaccagcac
     1021 cgacaatctc atgaatttca tcaataaaga gaatcacatc cttgcgattg cgaatttctt
     1081 ccattaattt ttgcatgcgc tcttcaaact gaccacggat acctgttccc tgaacgaggc
     1141 taaccacatc aagacgaatc acttgcttgc cttggagttt ttgaggaaca gtaccatcaa
     1201 taattttttg agccaaacct tctacaacag cagttttacc aactccaggc tcaccaatta
     1261 gcacaggatt atttttagta cggcggttga ggatctcgat aacgcgtgta atctcttcgt
     1321 cacgaccaat aacaggatca atattaccat ttcttgcaat atctgtgaca ttaatcccaa
     1381 actcttctaa taagcccttt gcttgctgat ttggtgtttg tggctgagca ggtcgttgtc
     1441 cgttgtagtt accaccatag cgtcctccgc catttccatt ttgccctgcc tgagtaggtg
     1501 gggtatttgg aagattacca aaagctctaa aattattcaa atcaccaaaa aagtcatcaa
     1561 agaaaggact tgtggatcta tcttgtgctc tatatcctgg ggttaggcca tttaaaatag
     1621 aattggcagg atcggatttc atgatttgat aacaattttg acagagatca acctgtcttt
     1681 gttttccatt aacacttgta taaagatgaa tagtagattc gtttaaatta caattttgac
     1741 acagcataat atacctccga gggtttatct aaaagtcaaa gtcatttagt caaaaatggt
     1801 caaactttat attcatatta tagcaccacg gcttgggaaa tcaagtaaaa agtttatcgc
     1861 tttagcgtta gcttttagag agtaacatga cagagtaact ttattaagtt tccaatgagc
     1921 aaatgtttaa aatcattgaa aagagtaaaa aaagtaaaat atcaataaat atattacttt
     1981 ccttgttagt ttcccttgtt ttgtgttaaa ataagaggga taaaaaacga ataggagaaa
     2041 cgaaatggaa tcacatttgg tgagaatcat caatcgtttg gagttgatgg tgacagatgg
     2101 cggtaatctc aagcgaaatt ttgaacgcga tggtgttgtt gttgcagaag ttgccttttc
     2161 aaatgatcct gaaaatggac ctgttttcac attgcgtgat gttgaagcgc gtgagtcata
     2221 ttcctttgat agcattgact tgattgcaat ggaaatctac gatttattat attaagcttg
     2281 acatagtaaa gagacttgcc tgaggcaggt tttttttatt gtcaaaaagt attgtattaa
     2341 tattcatcag atggtataat tatctttatt gtaatgaacc taggacttat gaggagatat
     2401 aaatggattt gtcatttttg cccaaatact gggcctactt taactacggt gtacttgtca
     2461 ccattatgat ttcaatcagc gttgtctttt ttggaaccct tattggtgtc ctggtaaccc
     2521 tgattaagcg tagtcatgtg aagccgttga cctgggtcgt taatctttac gtttggatct
     2581 ttcggggaac acctatggtg gttcaaatca tgattgcctt tgcttggatg cattttaaca
     2641 atatgcctac tattggtttt ggggttttag acttggactt ttcaagacta cttcctggaa
     2701 ttattatcat ttcattgaat agcggtgctt atatttcaga aattgttaga gcaggtattg
     2761 aggctgtacc aaaagggcaa ttagaagcag cttattcact aggtattcgt cctcaaaatg
     2821 ccatgcgtta tgtgattttg cctcaggcct ttaaaaatat tttgccagcc ttaggaaatg
     2881 aatttattac cattattaag gatagtgctc ttttacaaac cattggagtg atggaacttt
     2941 ggaatggtgc ccaatcggtg gtaacggcta cttattctcc aatttcccct ttactggtgg
     3001 ctgcttttta ctacttaatg gtcacaacag tgatggcaca gttattggca gtcttagaac
     3061 gtcacatgtc gcaaggaggt aatcattgat gactgaggct attatttcaa ttaaagactt
     3121 gcacaagtat tatgggcaca atgaagtgct taagggaatt gatttagaca ttatgccagg
     3181 agaggttgtg gtgattattg gcccttctgg ttctggaaaa tcgaccttac ttcgaactat
     3241 gaatcttttg gaagtaccaa ctaagggaca gattaggttt gaggggattg atattaccga
     3301 taagaagaat gatattttca gcatgcgtga aaaaatggga atggttttcc agcagtttaa
     3361 cctctttccc aatatgacta ttttagaaaa tatcacttta tcgccaatca aaactaaggg
     3421 aatggctaaa gcagaggctg acaaaacagc cttgagcttg ttggacaaag ttggattatc
     3481 agaaaaagcc aaggcttatc ctgctagtct ttctggtggg caacagcagc ggattgcgat
     3541 tgcgcgtgga ctggctatgg atccagatgt tttactcttt gatgaaccaa cttcagctct
     3601 agacccagaa atggtgggtg aggtcttggc tgtcatgcaa gatttggcta aatctgggat
     3661 gactatggtt attgtgactc atgaaatggg ctttgctaaa gaagtcgcag atcgtgtgat
     3721 ttttatggat ggcggcgtta ttgtggaaga aggaagtcct aaccaacttt ttgatctgac
     3781 taaagaagaa cgtacaaaag attttttgag tagagtatta taaatctgtt ctatgaatta
     3841 taaaaggaag tcctaaatca tttaggactt ccttttataa tttgctaagg cattttatag
     3901 gagattactt ttctttttgt taaaataaaa agaaatagtt gcattatttg catagaagaa
     3961 agggatctgt tatggtttca gtgatgtggt ttcgtagaga tttaaggatt gatgatcaaa
     4021 aagctttagc tcatgctatt gctagtcaat caccaacctt atgtgtgttt caatttaaca
     4081 aggagcaatt aaatctgcta catagtcgga atcaatctag ttttattact agcgttttgg
     4141 cttttagaaa gttattaaaa tgtgaaggta ttgacttata tctaatgtat ggagacttaa
     4201 tgacctgttt tgagcaatta ttgacacaat taaaagactg gacagatgtg tattttaact
     4261 atgatgaatc tgggtatggt cgtttacgtg atcaaaaagc agcacaattt ttcaaaaaaa
     4321 atgggattgc cgttcatacc taccaagatc actatttaca tggtagtcaa gagatcatta
     4381 accaatcagg tcagccttat aaagttttta cgccctacta taggatttgg caaaattatc
     4441 cgaaagagac tcctattaaa gtagaactat cgcaaggcag gtggttaaat cttgagacac
     4501 cagatgatgt tttaaggact gttgagagca ttaaagatga aaaatatcag gatgttgcaa
     4561 cttttgatga agcgtcaaaa caactaaatc gatttattca agaccaatta gcagcttatc
     4621 acgctaatcg agattttcca gcgcagctag ggacgagtcg gttgtctcct tttttaagaa
     4681 taggagctat tggcattcgc acggtatatc acgctgtgcg tcaagcacca aatagtctag
     4741 ggcaagctac ttttttaaaa gaattagctt ggagagactt ttacaatatg gtctatgtgg
     4801 cttatcctga tcagaaaact cagcctatcc aaaaagcctt ttctcaaatc gaatgggtta
     4861 ataatcctga ctggtttcag ttgtggaaag aaggtaaaac gggttaccct attgttgatg
     4921 ctgccatgct tcagttacaa aagacaggat ggatgcataa tcgtttacga atgatagtag
     4981 cttctttttt aactaaagat cttttatgtg attggcgttt aggagagcag tattttcaac
     5041 aacaactaat tgattatgat gctgccagta atatcggagg atggcagtgg gctgcttcca
     5101 ctggaacaga tgctgttcct tattttagaa tatttaatcc tgttactcaa ggtaagcgct
     5161 ttgatcccaa aggcgagttt attaaagcgt atttgcctca actagaacac gttccagaga
     5221 agtatctcca cgaaccgtgg aagatgccaa aaaaccttca agagagtgtg tcctgtatta
     5281 ttggtacaga ctatgctcaa ccaattgtcg atcatgctaa acaacgtgaa caagctattg
     5341 caaagtatga gtgggcaaaa gaaaaagcaa aaatagaatg actgttctca ttataagagt
     5401 ttaaacaaaa agttatcatg aattaagatt atttaattta aaaaataagt tgactttatt
     5461 tagattattt gatactattc agttgtctcc cttagagaaa gcgatttctt gttttggagc
     5521 tgatttttac catttggtct tagaatagga tgagtaatat gacttacaac gaggtatatc
     5581 aagaatggtt gcacaataat gatcttagtg atgatattaa agcagattta gcagccataa
     5641 aagacaatga ggctgagatt caagatcgtt tttacaaaac acttgaattt ggaacagcag
     5701 ggctaagagg aaaacttgga gcaggcacca atcgcatgaa tacctatatg gttggtaagg
     5761 cagcacaagc tttagctaat actattattg atcatggacc tgaagcggtt aaaaagggca
     5821 ttgcggttag ttatgatgtt cgctatcaat ctagaacatt tgcagaatta acatgctcta
     5881 ttatggcagc taacggtatt aaggcgtacc tttataaagg gattcggcca acaccaatgt
     5941 gttcgtacgc tattcgtgct ttgggatgta tctctggtgt tatgattaca gctagtcaca
     6001 atccccaagc ttacaatggt tataaagctt attggcaaga aggttctcaa attttggatg
     6061 acattgcgga tcaaattgca caacacatgg ctgcactaac tcagtatgag gaaatcaaac
     6121 aaatgccttt tgaaaaggct ctggacagtg gacttgttac ttatattgat gagagtattg
     6181 aagaagcata taaaaaagaa gtgcttggtt taacgattaa tgatactgac attgataagt
     6241 cagtccgagt agtctatacc cctttaaatg gtgttgggaa tctaccagtg cgtgaggttt
     6301 taagacgccg cggttttgaa aatgtttatg tggtacctga gcaggaaatg cccgatcctg
     6361 attttacaac agttggctat cctaatcccg aagtccctaa aacttttgca tattcagaaa
     6421 aattaggtaa ggcagttgat gctgatattt tgattgcaac tgatccagat tgtgaccgtg
     6481 tggctttaga agttaaaaat gcagtcggtg actatgtttt tctcaatggc aataaaatcg
     6541 gagcgctttt atcttactat atcttttcgc aacgatttga cttaggcaat ttaccggcta
     6601 atcctgtttt agtgaaatcc attgtaacag gagacttgtc acgggctatt gctagccatt
     6661 atggtattga aaccgttgaa acattgactg gttttaagaa tatttgtggc aaggcaaacg
     6721 aatatgatgt gacgaaacag aaaaactacc tgtttggtta tgaagaaagt attggttttt
     6781 gctatggcac ttttgttcgt gacaaagacg ctgttagcgc ctctatgatg attgtagaaa
     6841 tggcagctta ctacaagaaa aaggggcaga atttactaga tgttttgcag actatttatg
     6901 cgacatttgg gtattataat gaacgtcaaa ttgccttgga gcttgagggg atagaaggac
     6961 aaaaacgcat tgcgaggata atggaagatt tcagacagac accaatagct agtgttgcag
     7021 agatggcgtt agacaagaca attgatttta ttgatggcta tcaagatttt ccaaagcaaa
     7081 actgtctcaa attttacttg gatgatggtt cttggtatgc acttagacca tcaggaacag
     7141 agcctaaaat aaaattttat ttatatacta ttggccaaac acaagaaaat agtgcaacaa
     7201 aattagatgc tattgaagca gcgtgtcgca caaaaataaa tcaggttaat taaacatcaa
     7261 aaatggataa cacttttaca ctatttttcc atataaacaa ctacaaaagg aggtgagact
     7321 tttaggcacc ttctttttct tgtaggtgcc actaatcaat ttaatcgact caaaccaata
     7381 ctaaaaaata agtaagagta taaagaacag gcatctattt ttttgttata atagaaaaat
     7441 gaaattaaag ggagatgtca atgacagaac taattgatgg taaagcctta gctcaaaaga
     7501 tgcaacaaga gttagcagct aaagtcaaca atctaaaaca aaaaaaagga attgtaccag
     7561 gcttagccgt tattcttgta ggtgatgatc ctgctagtca ggtgtatgtc cgtaataaag
     7621 agcgtgcagc tcttactgta ggttttaaaa gtgagaccgt tagattatca gaattcattt
     7681 gtcaagaaga gcttattgca gtaatcgaac gttacaatgc agataacact attcatggta
     7741 ttttagtgca gctacccctg ccaaatcata ttaatgataa aaaaattatt ctcgccattg
     7801 atcccaaaaa agatgtggat ggttttcacc cgatgaatac aggtcacctt tggtcaggac
     7861 gtcccttgat ggttccttgt actccatcag ggattatgga attgcttcga gaatataatg
     7921 ttaaccttga aggtaaacat gccgtcatta ttggcagatc gaatatcgtt ggaaaaccaa
     7981 tggcacagct cttactggac aaaaatgcaa cagtcacgtt gacacattca agaacacgtc
     8041 aattagaaga agtatgtcgc tgtgcagatg tgttgattgt ggcaatcgga caaggtcatt
     8101 tcataacaaa acaatatata aaagagggtg cgatagtgat tgatgtggga atgaaccgtg
     8161 atgataatgg caagctaatt ggagatgtgg cctttgatga ggtggcagaa gttgcagcga
     8221 aaatcacccc tgtaccagga ggtgtcggtc ctatgacgat tgctatgttg ctagagcaaa
     8281 cttatcaatc tgctctccgt agtactcata aatagttatc attagacaat tcaacataag
     8341 agaatataag aagtcagcaa tggcttcctt ttttgttttt ctgctcacta tttattcaga
     8401 aaaatggtat aattgcatta gtatgcaaag ggactatgtc aggtccaact aagtgaaagg
     8461 tggcttgatg gcagattatt taaccgtcac tcatttgacg aaatatttaa aattaaaatt
     8521 tgaccgtgac ccttatttgg aacgggttta tctgactggt caagtgtcca attttcgaaa
     8581 acgaccaact catcaatatt tttccttaaa ggatgaaagt gctgtgattc aagccaccat
     8641 gtgggcagga gtctataaaa aactaggatt cgacctagaa gaaggcatga aaattaatgt
     8701 aattgggcga gtccaacttt atgaacctag tggctcttac tctatcgtga ttgaaaaggc
     8761 agagccagat ggtataggtg ccttggcttt gcagtttgaa caattaaaga aaaaattaac
     8821 ggcagaaggt tattttgagc aaaaacacaa gcagcctttg ccacagtttg tatcaaaaat
     8881 tggggtcatt acaagtccta gtggtgctgt gattcgagat attatcacaa ccgtttcacg
     8941 gcgttttcca ggggttgaga ttttattatt cccgactaaa gtacaaggtg atggagccgc
     9001 ccaagaagta gtggctaata ttcgaagagc caatcaaaga gaggatttgg acttgctcat
     9061 tgtcggccgt ggaggtggct cgatagaaga cctttgggcc ttcaacgagg aaatagtggt
     9121 tcaggctatc tttgaatcgc aacttccagt gatttcgagt gtgggtcatg aaacagacac
     9181 gactttagcg gattttgtgg cagatcgcag agcagccaca ccaacagcag cagcagagtt
     9241 agcaacgcct attacaaaaa cagatcttat gtcttggata gtagagcggc aaaatcgttc
     9301 ctatcaggcc tgcttgaggc ggattaagca acggcaagag tgggtggata aactctcgca
     9361 atctgttatt tttaggcaac cagaacgatt atatgatgcc tacctgcaaa aaattgatcg
     9421 ccttagcatg accctgatga atacgatgaa agatcgtctg agttccgcca aagaaaacaa
     9481 ggttcagttg gaccatgcct tggccaatag tcaattacag acaaaaatcg agcgatacca
     9541 agaccgtgtt gctaccgcta agcgtttact gatggctaat atggctagtc aatatgatag
     9601 tcagctggct cgctttgaaa aagcacagga tgctttgcta tcactggatg ccagccgaat
     9661 tatcgcccgt ggttatgcaa tgattgaaaa aaatcaagca ctggtagcct ctgtaagtca
     9721 gataacaaag ggtgatcagt taaccattaa gatgcgtgat ggacaattag atgtagaggt
     9781 aaaagatgtc aaaaacgaaa acatttgaag aaaatttaca agatttagag actattgtga
     9841 acaaacttga aaatggggat gtccctttgg aagaagctat ttcagaattt caaaaaggaa
     9901 tgcttctctc aaaagaactt caaaagacct tacaagcggc agaaaaaaca ctcgtcaaag
     9961 tgatgcaagc tgatggcaca gaagtagata tggatgatta atggacaaat tagctagaat
    10021 tgacgaagcc attcgtcgct actataagac gactagtaac ggtgtatctg aagagttgat
    10081 tgatgctatc ttgtattctg ttgacagtgg tggcaagcgc attcgcccac ttatcttatt
    10141 agagatgatt gagggatttg gggtatcgtt acagaatgct cattttgatt tggctgcagc
    10201 ccttgaaatg attcatacag gaagtctgat tcacgatgat ttgccagcca tggataatga
    10261 tgattaccga cgtggtagac tgaccaatca caaacaattt ggagaggcta ctgctatttt
    10321 ggcgggagat agcttatttt tagacccctt tgggttaata gctcaagctg aattaaatag
    10381 tgaggttaaa gtagccttaa tccaagaact ttccctagct tcaggaactt ttggcatggt
    10441 tggtggtcag atgctagata tgaaagggga aaatcaagct ttgagtctcc ctcagttgtc
    10501 actgattcac ctcaataaaa ccggaaaatt attaactttt ccttttaaag cagcagctct
    10561 tgtaacagac caggctatta ctgttcgtca acaactagag caggcgggaa tgctcatcgg
    10621 gcacgccttt cagattaggg atgatatttt agacgtgaca gctagctttg aagatcttgg
    10681 aaaaacgcct aaaaaggatt tatttgcaga aaaagctact tacccaagtt tactggggtt
    10741 agaagcctcc taccaactgt tgacagagag tttagatcag gctttgacga tttttcagac
    10801 actagaaaac gatgtaggct ttaagcctca aataattaca aaactgatag aagggttacg
    10861 acttaatgcc taaagaaaga gtagatgtgt tagcctataa gcaaggatta tttgagacca
    10921 gagagcaagc taagcgtggt gttatggcag gcctagtggt ctctgtgatt aatggccaac
    10981 gctatgacaa gccaggtgac aaaattgacg atgggactga gttaaaactt aaaggtgaaa
    11041 aactcaaata cgtcagtcgt ggtggattaa aactggaaaa ggggctgcac gtttttggtg
    11101 tatcggttgc taatcaaatt gggattgaca ttggcgcttc aacaggtggt tttaccgatg
    11161 ttatgttaca agatggggcc aaactagtct atgccgtcga cgttgggact aaccagttgg
    11221 tatggaaact cagacaagat ccacgagtga gaagtatgga acagtataac tttcgttatg
    11281 ctcagccaga ggactttgat gagggacagc ctgtatttgc cagtattgat gtgtccttta
    11341 tttctctcag cttgattttg ccagccctgc ataacgtttt gtcagatcag ggacaggtta
    11401 ttgctctcat taaaccgcaa tttgaagctg ggcgcgagca gattggtaaa aaaggcatcg
    11461 tcaaagacaa acagattcat gaaaaagtga ttcaaaaggt catggatttt gcctcaggtt
    11521 acggatttac agttaaggga cttgattttt cccctattca gggtggtcat ggcaatatcg
    11581 aatttttagc tcatcttgct aagtcacaga cacctgaaac gttagccccg catttgattc
    11641 agaaagttgt tgccaaggca cataaggagt ttgagaaaca tgaaaaagag tgaacgactt
    11701 gaattaatca aaaaaatggt tttgacgcat ccaattgaga cgcaacatga cctcttaaga
    11761 ttattggcag agcatgggtt agaattaacc caggctacta tttcaagaga catgaatgaa
    11821 attggtatag taaagattcc ttctggcagt ggacgttaca tctatggtct ttctcaagat
    11881 agtcgtaaaa agattattca aggaccacgt tctattaaaa gtaccatttt ggctatttcg
    11941 gacccgacag caggacttga gcaacatctt tatttaaaag tagttcctgg aaatagcaaa
    12001 ctcataaaac gttacttgtt aactgatttt ccagaagcta tttttagtct tattgcagat
    12061 gatgacagtt tacttttgat tgctaagtca gcagcagatg ccgataagat tcgtcaggaa
    12121 gtcttgctct ggatgcaggg gcaaacttag aagaacaggg agaaagtgat gctattagaa
    12181 atttccatta aaaattttgc cattattgag gaaatctctc tgaattttga aaatggcatg
    12241 acagttttga caggtgaaac aggagctgga aagtccatta ttattgatgc catgaatatg
    12301 atgcttggtg cgcgtgctag tacagaagtg attcgtcgtg gagctaataa agcagaaatt
    12361 gaggggttct tttcggtaga tgctacccca gaactggttg cgtgtctgga atcatcaggt
    12421 attgctatgg aagaagaact gattattcgc cgggatatct ttgccaatgg cagaagcgtg
    12481 agccgcatta atggtcagat ggttaaccta gccaccttaa aacaggtcgg acagtttttg
    12541 gtagatattc atgggcaaca tgaccaagaa gaattaatgc gaccacagct ccaccagcaa
    12601 attttggacg cttttggtga taaggctttt gagcaattga aagagaacta tcagcttatt
    12661 tttgatcgtt ataaaagctt gcgtcgccag gttattgaca aacaaaaaaa tgaaaaagaa
    12721 cataaagatc gtatcgacat gttggctttt cagatagcgg agattgaagc tgctgccttg
    12781 agtcgaggtg aggacgaccg gttaaatcaa gaacgtgatc gcttaatgaa ccacaaacaa
    12841 attgctgata ccctgaccaa tgcctacgtc atgctagata atgacgattt ttcaagtcta
    12901 tccaatattc gctctagcat gaatgactta ctatcaattg agcagtttga ttcagagtac
    12961 aaagggatgt cgacttcgat ttctgaagcc tattatattc ttgaagaagt gagcaagcaa
    13021 ttatcagata ccattgacca actggatttt gatggtgggc gattacagga aattgaattt
    13081 cgcttggata cactcaatag cttaacccgt aaatacggtg gtaatgtgaa tgatgtgctt
    13141 gactactacg ataatattat caaagaatac cagctattaa caggagacga cttgtcttca
    13201 ggggatctag aatcagaatt gaaagcctta gaaaaacaat tggttgctgc agccactaac
    13261 ttgagtgaac gccgccatca attggcagag caattagaag ctgagattaa agctgaactt
    13321 aaggaacttt atatggaaaa agcagatttc aaagttcact ttaccacatc aaaattcaat
    13381 cgtgatggta atgaaagctt ggaattttac atctctacta atccaggcga aggatttaag
    13441 ccccttgtca aggtggcttc tggaggagag ttatcacgcc ttatgttagc catcaaggct
    13501 gctatttcta gaaaagaaga taagacgagt attgtttttg atgaagtaga tacaggtgtt
    13561 tctggtcgcg ttgcgcaagc cattgcccaa aaaatttata aaattgggcg acatgggcaa
    13621 gttctcgcta tttcacactt acctcaagtt attgctattg ctgattatca gtattttatt
    13681 tctaaagaaa gtaaagaaga gtccaccgtt tctaaggtaa ggttattaac tcctgaagaa
    13741 cgtgtggaag aaattgctag catgattgca ggaacagata tgacacaagc tgctcttacg
    13801 caggctcgtg aactacttgc caaacattaa gattatgttt ggtatttgat attttcataa
    13861 ttagatgaca ttgatttttt aggtgctaaa ataatcattg tgtcagacca tttttcctct
    13921 gttaatctgg cttggtttgc cttgacaaac ctctgagaaa tagtagaata gataagagaa
    13981 ttttaagctg aggggataga aatgactagt aaaaaagcgt gtttatcaag catcattgtg
    14041 ttagcaagtt taacgtgtgg aaatgatact gttagtgcca atcatctctc agcaactgga
    14101 gataagtttg atgattgctc aacacttgtt gaaaaagatg tggcccctaa agatgaactt
    14161 gagatgttag catggtcctc gtctcaaaca actgatgatg ctgacagaga ctatgaagat
    14221 tttctcgatg atgattcttt taatgatgat tcttttattt ctcaaaatga aactgataag
    14281 atgtttgaga atttaactga tgataggtta ttagatgaat tagatgaaga agatacaatt
    14341 gagccagagc aaaatgtaat aatgcctagt gacgatgagc tatttgattt aactgatgct
    14401 gttgagacac gccttactgt ttctagtgct ccccatttag aggctgaatt gccgaaacca
    14461 catttgagga gcctatcaga tacagcactg cggtctggtg aaattagagg acatttagat
    14521 aacaaactgg acgctttgtc tgtaacagct acaaagttag cattaacgat ggctcaaaaa
    14581 tttgatttga caacgcatgt ctattctata ggtgaaagct ttagtgaagt attagctgct
    14641 cattatgaag acagaaaagc agaatcagct ttttctaaga aaaagagatt tcaccttcct
    14701 attgctactc cagatgttgt tatagaggag ttaaggcgcc tggtctcttc tattggaagt
    14761 tcaaaagaag atgtttcagt tccttataat cggaagctag gtatggcagt tgcaaaaaga
    14821 aaaatagccc tgccacaaac gggagaggag ttctcttatt atccagcttt acttggttta
    14881 atgatattag gattaacgcc gattatgata ccaaagaaga taaataatta ggtaataagt
    14941 tattgtagtt gtaaaagacg gggcaaatgt tcaagcgtga aaaggttgac ttttgcctcg
    15001 ttttgtatct cgttattgct aaggcatttt cttcgagcag ctagtgctcg ccatacctat
    15061 ctaccgagtg actattcaat agcactacca gttttgcttt atttagcaag gagtgttaga
    15121 ataaagaggt gagaaactag tttagaatgg aaaaatacat gggaactata aaaattgtta
    15181 cagattcatc aataactatt gaaccagaat taattaaagc tttagatatt actgtagtgc
    15241 ctttatctgt catgattgat agcaaattat attctgataa tgatttaaaa gaagaaggtc
    15301 attttttgag tcttatgaag gctagcaaat cccttcccaa aaccagtcag ccaccggttg
    15361 gtctctttgc tgaaacttac gaaaatttgg tcaaaaaagg agtaactgac attgttgcta
    15421 ttcacctctc accagcttta tctggtacta ttgaagcttc ccgtcagggg gctgaaatcg
    15481 ctgaggcccc tgttacagtt ttagattcag gatttactga tcaagccatg aaatttcagg
    15541 tcgttgaggc agcaaagatg gcaaaagcgg gtgctagttt gaatgagatt ttggctgctg
    15601 ttcaagctat caaatcaaaa acagagctct acattggtgt atctacctta gaaaatttag
    15661 taaaaggagg tcgtattggt cgtgtgacgg gtgttttgag ctcgctactt aatgtcaaag
    15721 ttgttatggc acttaaaaat gatgagttaa aaacgcttgt caaaggtcga ggtaataaaa
    15781 catttacgaa atggttagat agttacctcg ctaaaaatag tcatcgcccg attgcagaga
    15841 tagctatttc ttatgctggt gaagctagcc tggcattaac cttaaaagaa agaatcgcag
    15901 cttattacaa ccactctatt tcggttttag aaacaggctc tatcatccaa acacatacag
    15961 gagaaggagc ttttgcggtt atggttcgtt atgaataatc gtcatttatt tagtgggata
    16021 tttttctttg ttattagttt atgtctggcc tttttattgc taaatattat tatccctaag
    16081 tcaaattcac gtttgaaaaa gagtgatttt ctgaaaaaag aacaagtagc tatccaatat
    16141 gttgctatag gagattcatt gacagaagga gtaggtgatc taactcatca aggtggtttt
    16201 gttcctttgt taacgaatga tctcagtgaa tattttaagg ctaatgttaa tcatcaaaat
    16261 tacggcgtat ctggtgatac cagtcaacag attcttgata ggatgataaa acaaaagcag
    16321 atacagttat ctttaaaaaa agcagatata atgacgttaa ccgttggtgg taatgatgtt
    16381 atggcagtta ttcggaagaa tttagcggat ttgcaagttt ctagttttag aaagccagct
    16441 cgtcagtatc aaaaacgatt aagacagatt atcgagttag ctagaaaaga taataaagat
    16501 cttcctattt ttattttagg catctataat ccgttttatt tgaattttcc agaactaact
    16561 gatatgcaaa aagtgattga tgactggaat atcaaaacta aggaggttgt tggagaatac
    16621 gatcgtgtgt actttgtgcc aataaatgac ctcttgtata aagggataaa tggacaagaa
    16681 ggaattgtcc attcttcagg agatcaaact acaattgtca atgatgcctt gtttactggg
    16741 gaccattttc acccaaataa tactggctat caaatcatgt caaatgcagt aatggagaaa
    16801 attaaaaagc atgaaaaaaa aatcaaacct taattggtgg aagtggagct tcttatgtct
    16861 gcttgctttt aataccgcct ttttgatggt tattgcaagt cgtctcattc aagtaagaga
    16921 acctgagtca gaactgattg ctaaaaaacc agttaaaaat atcaaaattg gtacatttgt
    16981 cacaaccaga gaacagctta acgaaactgt agcaagttat cttaaagatt atcagactaa
    17041 aaaaatgtct tataaatttt atgcgacatc ttcttctatt ttgtttgaag gaacttatca
    17101 attattaggg tatgaagtcc ctttatatat ctattttcaa cctcatcgtc tagaaaatgg
    17161 agctgtccaa ctacaggtga tctctttttc agtgggaact ctgccactcc cagaaaaaga
    17221 tgttttgcag tatttgaaat caagctacaa attgccaagc tttgttaaag tgatgccaaa
    17281 tcaatcagct atcgttgtta atttacaaga tatccaaaat gatgctaagg tttatttaaa
    17341 agccaagaaa attgatttat ttaatgatga aatcagtttt aacatctata agaaataagc
    17401 tcaaataacg ttaaatactt gacctaatcg ctttttttag atatgataat atctgttaaa
    17461 gcattaagct tactaattat ttggaggatt tgttaaatgg ctaacaaaca agatttaatc
    17521 gcaaaggttg cagaagcaac tgaattgact aaaaaagatt cagcagcagc agttgatgcc
    17581 gtgttttcta caatcgaagc tttccttgct gaaggtgaaa aagtacaatt gatcggtttt
    17641 ggtaacttcg aagtacgcga acgtgcagct cgtaaaggtc gtaacccaca aactggtgca
    17701 gaaattgaaa ttgcagcttc aaaagttcca gccttcaaag ctggtaaagc tcttaaagac
    17761 gctgttaaat aattcgtcta gaaaaatctt gttgctatcg atgtttattg atagcgacaa
    17821 ggttcttttt ttatatttgg ggcataaagt tagtagtttt ctcttcagtt aagccaagtt
    17881 ctaatgcatg cactctataa tggtcagtca tccttctatt agtatatcat ttccgcaagt
    17941 agggggagtt tttttcatat ttgcaaatag ttgaagttag gatttttgaa agcaaaaggt
    18001 aagtttaaaa aagtttgttt agaatgtttc taaattactt gacttattta tttagaatta
    18061 ttataattaa tatatcttaa agattgaata acttttaatt gcgagggaac taaaaatgac
    18121 aatcagacaa tggatggcag accatcttca tttaatggag actttagcgt gtttggtatt
    18181 aataataatt ggcctagcct ttttacactc atttccacaa gtggcttccg ctattttcat
    18241 tacagccttc ttgattggag gatatgcgtc tgcaaaaacg ggtatattgg atttggtgaa
    18301 gaacaaacac ttgtcagtgg atattttgat gattttggca gctatcggtg ctggaattat
    18361 cggctattgg ctggagggtg ctctgcttat ttttatcttt tcgttgtcca atacgcttga
    18421 agaaatggcc atggaaaaaa gtaaggatgc tatttcaggc ttgatgtcct tgacgccaga
    18481 tacagctcgt caataccaag aagatggcca tattttagaa gttgagacca gatccttgag
    18541 tgttggtgac cgtttacaag ttcgtaaagg cgaagctgtt ccaattgacg ggcagttgct
    18601 tagtcctttt ggtcaatttg atgaatctat ggtcactggt gagcctatca ctgttgataa
    18661 ggcagaaggc caggatctga ttgggggaac cattaaccaa ggacaaacca tagatatgtt
    18721 ggttactatt gaaaatgacg atactctctt tgccaagatt attaatctgg tggaatctgc
    18781 ccaagaaaag aaaagtaaaa ccgccacctt tatcgaaagc ttagaagatg gttatgtcaa
    18841 atttgtgctc gtccttattc ctgcctttat cctctttagc cactttgtgc tttcttggac
    18901 ttggttggct gctttttacc gagggatgat tctcttaaca gtagcttcac catgtgccct
    18961 aattgccagt tccacacctg ctagcttggc tgctatttct cgtgcagcca ggaagggatt
    19021 gattatcaaa ggaggggata ttgtcgataa catgggagat attaaggctg ttgtcatgga
    19081 taaaacggga acgctcaccc aaggaaaacc ttctgttgtg aatgctcatt atttggaaga
    19141 tgagttgctc gtgaatagac tggtaaaagg agcagaggct gctagtaccc atcctatttc
    19201 taaagccttt cttgaataca ctgaaaaatt ggagccactg acctttgacc acttagaaga
    19261 aatttctggg aaaggttttc agggcttcta tcaagggcaa gaatggcgca ttgggaagaa
    19321 atcctttatt ttagagatgg tgccagatct ttcagctttt gaagaaacga ttcaggtaga
    19381 agaaagtcaa gggaaaactc ttatctttgt ttctcgcgac aaccaactag tagcctacta
    19441 cgctttgtta gatgacatca aattagagtc aaaacgtgcc attgaagctc ttcatgctat
    19501 gggaatcaag acggtcatgt taacagggga ccaagaacga actgccaatt atgtggcaca
    19561 aaaacttggt attgatgaag tggtagccaa ctgtatgcct caagataagg tggctaagtt
    19621 agcagaatta aagaccaaat atggttttgt ggccatggta ggagatggta ttaatgatgc
    19681 tcctgccctt gctcaagcag atgtttctta tgctattgga tcaggaacag atattgcaat
    19741 ggaaagtgca gacagtgtga ttatggatga cttgactcgt attccatttt cgattcaact
    19801 ttcccgcaaa atgaagacca ttatcaaaca aaatattgtt tttgccttat ctgtgattac
    19861 cttattgatt ttagccaatg tttttcaggt agttaacttg ccgcttggtg ttgttggaca
    19921 cgaaggctca acgattttag tgattttaaa tggcttgcgt ttactttctt ttaaataatt
    19981 tagaaacttg cttgaaaact tgaaagtgtt tataataata ctaagactta tgaaagagaa
    20041 aaccgccgta tgtttactat tatgatcatt agtttagctg tattttcaat aattaaaatc
    20101 attctatttt tctattttta tcagcaattt gagcgcaagc aaaaaagaaa agttgaatca
    20161 aacaaccagc aaagacagtc caaagaaagt gacgctgtcc cttcaaaaaa attcccattt
    20221 taaaaagctt ggaaagtgta caaagtgcac atttccaagc ttttttgact attaaggtta
    20281 atctttgtaa cgtaagttcc cacgaaaatc gtctaagctt tggtagcctt tttctaccat
    20341 aatcgttttt agttccttag tgacccgttc aaagatagct ggcccttctt gatgaagggc
    20401 tgtcccaatt tggaccatgc tggcgccaca aagaatatgt tcaaaggcat cgcgtcctgt
    20461 tttaactccc cctgtaccaa taatgtggat agaaggtttt aagcgtttgt aaaacgcatg
    20521 gacattggct agagcagtag gtttgatgta atctccacct ataccaccaa agccattttt
    20581 aggcttgatg aggacttgct catcctcaat gaccagccca tttccgatag aattgacaca
    20641 gttaacaaag gaaagggggt atttattgaa aatcgcagcc gcttgatcaa aatgcacaat
    20701 gtcaaaataa ggaggtaatt tgataccaag aggtttagtg taataggtaa agatgttctc
    20761 tagaagttga tcagtggttt caaaatcata agcaatctgt ggttttcctg gaacgttcgg
    20821 gcaagaaagg tttagttcca ctagaccctc ataatctgaa gccatgatgg ctt
//
