LOCUS       NZ_CAAHMR010000001     10217 bp    DNA     linear   CON 11-MAY-2024
DEFINITION  Streptococcus pyogenes strain Bra016, whole genome shotgun sequence.
ACCESSION   NZ_CAAHMR010000001
VERSION     NZ_CAAHMR010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2019) WTSI, Pathogen Informatics, Wellcome Trust
            Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900984225.1-RS_2024_05_11
            Annotation Date                   :: 05/11/2024 02:59:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,760
            CDSs (total)                      :: 1,704
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 56
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 22 of 43
            Pseudo Genes (incomplete)         :: 20 of 43
            Pseudo Genes (internal stop)      :: 16 of 43
            Pseudo Genes (multiple problems)  :: 13 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:18:04
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 643010
            Orig. end                         :: 653227
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CAAHMR010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10217
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10217
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10217
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5217](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5217)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            182..424
                     /locus_tag="E9727_RS09340"
     CDS             182..424
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285423.1"
                     /locus_tag="E9727_RS09340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002985713.1"
                     /transl_table=11
                     /translation="MKQQGHTVMMCLNKTLTIKLSAPLTSNDLVPLSTNEVIIMEPLLH
                     LLPKVVPVLIHLVDKANVSWLTVILQLPILLKTIW"
     gene            946..1215
                     /gene="stcA"
                     /locus_tag="E9727_RS03245"
                     /old_locus_tag="SAMEA3919085_00656"
     CDS             946..1215
                     /codon_start=1
                     /gene="stcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /locus_tag="E9727_RS03245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_00656"
                     /product="quorum-sensing system protein StcA"
                     /protein_id="WP_011054255.1"
                     /transl_table=11
                     /translation="MTKKQFYLILAISILCFILAVSPKPPILKHTIERKRTAMTIGHSK
                     QNLHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(1564..1962)
                     /locus_tag="E9727_RS03250"
                     /old_locus_tag="SAMEA3919085_00657"
     CDS             complement(1564..1962)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /locus_tag="E9727_RS03250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_00657"
                     /product="hypothetical protein"
                     /protein_id="WP_011054254.1"
                     /transl_table=11
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLED
                     DAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTGIT
                     YGDDTLADLTHHLLHSDGVIPSLNDKSA"
     gene            complement(2055..3125)
                     /locus_tag="E9727_RS03255"
                     /old_locus_tag="SAMEA3919085_00658"
     CDS             complement(2055..3125)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /locus_tag="E9727_RS03255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_00658"
                     /product="hypothetical protein"
                     /protein_id="WP_011106865.1"
                     /transl_table=11
                     /translation="MMMLRDVKVKTSSLLVGCAATLLVSLSGTVAADSVHSSDRRFGVG
                     YKEETTSQFSSDQPSQVVLYRGEANTVRFTYTNQMSLMKDIRIALDGSDKSLTAQIVPG
                     MGHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMHNTMYKGTVFLV
                     QPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGGSGALPDNIKVSIN
                     PKGGLATITYGAGSTDIIPPAVLWKKGSVKEPTEADQSVGTPTPGIPGKFKRDQSLNEH
                     EAMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNEDEKEKPASDVKVRPAEVGSWLEPA
                     TALPSVEMSAEDRLKS"
     gene            complement(3376..3774)
                     /locus_tag="E9727_RS03260"
                     /old_locus_tag="SAMEA3919085_00659"
     CDS             complement(3376..3774)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /locus_tag="E9727_RS03260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_00659"
                     /product="hypothetical protein"
                     /protein_id="WP_011106866.1"
                     /transl_table=11
                     /translation="MRNILRFLGIVIISAVILFSIGSFYDLPLMKNIQLICWSFILDLF
                     VFVYKQRQTTEVLTWYQVVKQFWLFIKCTILIPTLIAFIIIGCLTSISEVSIYFYLHLI
                     VVCYTIGMILSLGRLISPNHSIFNKLKK"
     gene            complement(3968..4249)
                     /locus_tag="E9727_RS03265"
                     /old_locus_tag="SAMEA3919085_00660"
     CDS             complement(3968..4249)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528350.1"
                     /locus_tag="E9727_RS03265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_00660"
                     /product="hypothetical protein"
                     /protein_id="WP_134984498.1"
                     /transl_table=11
                     /translation="MKILDIIFNLSDRKMIFLENVEKLGGYIVPFTLIILSVAYNFRHP
                     YPIWAAVLGGISLIVIFIYFILIVYLYLRYRYLVWKKIPEVKINSTEL"
     gene            complement(4299..4472)
                     /locus_tag="E9727_RS09300"
     CDS             complement(4299..4472)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073350.1"
                     /locus_tag="E9727_RS09300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bacteriocin"
                     /protein_id="WP_306460903.1"
                     /transl_table=11
                     /translation="MAIINGGGKDGANIFLTGMAGAAQGVTVCAQTGVFIPWQGYILCG
                     AAGAATNIIWPH"
     gene            complement(4788..4988)
                     /locus_tag="E9727_RS03280"
                     /old_locus_tag="SAMEA3919085_00661"
     CDS             complement(4788..4988)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /locus_tag="E9727_RS03280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_00661"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_003060803.1"
                     /transl_table=11
                     /translation="MTTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHPV
                     LTGGCVVGFTVGGAYLGYTAN"
     gene            complement(5001..5217)
                     /locus_tag="E9727_RS03285"
                     /pseudo=""
     CDS             complement(5001..5217)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016754.1"
                     /locus_tag="E9727_RS03285"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="Blp family class II bacteriocin"
                     /pseudo=""
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2e-12, bitscore:
                     42.9, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIKKLETFHQMTIEKLAKVEGGKNNWQANVSGVIAAGSAGAAIG
                     FPVCGVAWS"
     gene            5534..6145
                     /locus_tag="E9727_RS03290"
                     /old_locus_tag="SAMEA3919085_00662"
                     /pseudo=""
     CDS             5534..6145
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003138385.1"
                     /locus_tag="E9727_RS03290"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_00662"
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTQFTTELLNFLAQKQDIDEFFRSSLEIAMNDLLQVELSAFLGYE
                     TYEKEGYNTGNSRNGTYSRQFETKYGLVNLIIPRDRNGEFSPVLLPSYARREDHLEEIV
                     IKLYQTGVTTREISDIIKRMYGDHLFLSGSLVTKRL"
     gene            6269..6523
                     /locus_tag="E9727_RS09200"
                     /old_locus_tag="SAMEA3919085_00664"
     CDS             6269..6523
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990779.1"
                     /locus_tag="E9727_RS09200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_00664"
                     /product="hypothetical protein"
                     /protein_id="WP_111689649.1"
                     /transl_table=11
                     /translation="MSGLFYHKSYFFKSEQVYDLLNEIRMRYPHTHIILIGNHINYEEI
                     FKNHYRVFGVIDTTSHKSLRSIRDQIQLYLDELYNSNNG"
     gene            complement(6828..7304)
                     /locus_tag="E9727_RS03300"
                     /old_locus_tag="SAMEA3919085_00665"
     CDS             complement(6828..7304)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /locus_tag="E9727_RS03300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_00665"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_014635332.1"
                     /transl_table=11
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTDDDGKVLMQLRGDKKTW
                     AIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFIYE
                     LTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
     misc_feature    7261..7277
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.42"
                     /tool="antismash"
     gene            complement(7324..8220)
                     /gene="era"
                     /locus_tag="E9727_RS03305"
                     /old_locus_tag="SAMEA3919085_00666"
     CDS             complement(7324..8220)
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="era"
                     /gene_functions="other (smcogs) SMCOG1227: ribosome
                     biogenesis GTP-binding protein YsxC"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /locus_tag="E9727_RS03305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_00666"
                     /product="GTPase Era"
                     /protein_id="WP_002985743.1"
                     /transl_table=11
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIMG
                     IYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDDMI
                     IERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTLIKL
                     LTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKRDEET
                     DKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVKVKKNW
                     RDKKLDLADFGYNEKEY"
     misc_feature    8273..8288
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.57"
                     /tool="antismash"
     gene            complement(8340..8747)
                     /locus_tag="E9727_RS03310"
                     /old_locus_tag="SAMEA3919085_00667"
     CDS             complement(8340..8747)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /locus_tag="E9727_RS03310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_00667"
                     /product="diacylglycerol kinase"
                     /protein_id="WP_002985746.1"
                     /transl_table=11
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLLS
                     ACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNAKD
                     MAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            complement(8728..9225)
                     /gene="ybeY"
                     /locus_tag="E9727_RS03315"
                     /old_locus_tag="SAMEA3919085_00668"
     CDS             complement(8728..9225)
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ybeY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /locus_tag="E9727_RS03315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_00668"
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="WP_134984801.1"
                     /transl_table=11
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTDKEEKEMSVTFVTN
                     ERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGELFI
                     SIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAYGLT
                     RQ"
     misc_feature    complement(9239..9253)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 18.45"
                     /tool="antismash"
     gene            complement(9384..9959)
                     /locus_tag="E9727_RS03320"
                     /old_locus_tag="SAMEA3919085_00669"
     CDS             complement(9384..9959)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /locus_tag="E9727_RS03320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919085_00669"
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="WP_009880369.1"
                     /transl_table=11
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIVA
                     QETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFAAK
                     WHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPSPRN
                     QLWLAKNPWFEQDLLPILQKRVEAILTK"
ORIGIN
        1 tcatggatat cctgattatc gttggtggat aacctttcgc ttaaaaacaa tccacttttg
       61 ggaaaccctt atatttagtt tgtaacagga aaactaatag atattaaaaa gagtgttagc
      121 gacctagcgg cagctttact cttaagtgcg tttgctgcca ttgacagcaa ggtcaaagct
      181 gatgaaacag caggggcata ccgttatgat gtgtctgaac aaaacactta ctatcaaact
      241 atcagctcca ttaaccagca acgacctggt gccactctcg acgaatgagg tgattattat
      301 ggaaccctta cttcatttac ttcccaaagt cgtaccagta cttatacact tggtagacaa
      361 agccaacgta tcatggttaa cagtgatatt acagttacct atactcttaa agacaatttg
      421 gtagcccttc ataaggaata taaaaccttt gtcaaaaaaa tattacagat aaaccgatca
      481 ccagtgacta cgacaaggct aaggttattt ataaccattt aatcaacgaa tacacttacg
      541 ccacaaaata acttagcatc aatcgcaaaa ccgtaagcag catcaacaat catactccta
      601 aagctcttta tcgtggcaag tgtggtgttt gtcaagctta tgccgtcgtg tttaaagata
      661 tggcagaagc tgcaggctta tcagcttggt atgtgataaa aaaggattga atgagcacca
      721 tgcttgaaac atcgttacca ttcatggtgt taattcctac gttgatgcta cctggaatga
      781 ttcagcatac accacaaggt atttccttgc tggaaaagac gtgaggaata ctgagtaccg
      841 tttagatagc cagtacaacc acttggctaa tgctatttta aataccagct accaaaaagg
      901 ctaaactaac aacaactaca tttttcaact agaaaggcct aaacaatgac caaaaaacag
      961 ttttatctta tcttagctat tagcatactg tgttttattc ttgccgtcag ccctaaacca
     1021 ccaattctta aacacactat tgaaaggaaa aggactgcga tgaccatagg ccactcaaaa
     1081 caaaaccttc acaaaaaaga agcccttaaa cacaaaaaaa acaaaaataa ggctcttttt
     1141 cttccaagtg gggatagcgc tatcaaatct gttattagca tgacagttag actcctgtta
     1201 gatgaccaat cttagctctc ctaaaatcag tggacactac catttcggta gtgttctttt
     1261 tttaagctaa tctcatcacc acgctttctt caagctaaaa aactgatcat gaaagagcaa
     1321 ccccatcacc ctaacagccc actttgcccg cactttctta aaaagttagg agaaaacgat
     1381 ataccaatta ttttctgatc ccagctccct atcattaaca agatgtgtag cacggaccta
     1441 catacaggta agctagagtc attctcgcta tttcggacaa aaagaggccg gtagttccct
     1501 gacctcttct ttatttatct tgtaacactc ctcttcttag cgagacttac tagcacataa
     1561 ttcttaagca ctcttatcat ttagactagg aataacacca tctgaatgaa gcaaatgatg
     1621 cgtcagatct gccagagtat catctccata agtaatccct gtttggtgct gtttctgatg
     1681 aaaatcagat gactccttaa ctttagcagt cacctgctgg tcacttggat gaaaattaat
     1741 ttctctcttg gcttcagccg caactggctc gaggaacaac ttattgacat tacggtcttc
     1801 ctccaaatga ttaaggatag cagcatcatc ttctaacgaa agaaccttct tgggtaacaa
     1861 aaaggggtta tttagccttg gctcagtaac gacttccttg gctactcgtg atagtttatt
     1921 attgtcttcc tcaggtatca aacggttagg caatcgatgc aattcttccg gctatgaggc
     1981 gctcacagct acttcagaag aaacctccaa gccctttcct gtttgcctgt ctaaagcaac
     2041 cggcaaatta ttaactaact ttttaacctg tcctcagcgc tcatttcaac actaggaaga
     2101 gctgtcgctg gttctagcca gctaccaact tctgctggtc ttaccttaac atcgctggca
     2161 ggcttctcct tctcatcttc attaggtcta aaaggctcaa accgccctgt tgatttttca
     2221 tctatgacct ttatattgtc ttttactaca tgagacagcg gttcgacatt taccatagct
     2281 tcatgctcgt taaggctctg gtctcgtttg aatttaccag gaataccagg agtcggtgtt
     2341 ccgacagatt gatcggcttc agtaggctct tttacggagc cttttttcca taaaactgct
     2401 ggaggaataa tatccgtaga gccagcccca taagtaatcg tggccagccc cccctttgga
     2461 ttaatagata ctttaatatt gtccggtaag gctccactac ctcctgtggt taaaagggca
     2521 cccgcatttt taatgagagt tgtcattcgt tcaataccag caacgccaat atggttcaag
     2581 tcatctactg gtaactgatc aacagatttc atggtgtatt tccaagcttg cggttgaaca
     2641 agaaaaacag ttcctttata cattgtgtta tgcatatcat caatgacttt gaaatacctt
     2701 gtatatttgg tttgtacatt agggttagca acgggaacag tgctttcagg aactcctgac
     2761 atcgtaaaaa tccctctagc agaagtttga aagccctcat aaacatgacc cataccagga
     2821 acaatctgag cggtcaaaga cttatcagaa ccatccaaag caatgcgaat atctttcatc
     2881 agagacatct gattggtata ggtaaaccta accgtgttag cctcacctcg atacaaaacc
     2941 acttgggagg gctgatccga agaaaactga gaggttgttt cttccttata gccaactcca
     3001 aaacgtctat cagacgaatg gactgaatct gccgcaactg taccactcaa actgactagt
     3061 aaagtagccg cacaaccaac taataaactg ctcgttttaa ctttaacatc tcttaacatc
     3121 atcatgttcc tttcattatt ttttatttag gaattcgtca tactatattt tctcatatta
     3181 gtctaacaaa aaacccgtaa ttctatataa atcatataaa atgacgagct ttggcaaaga
     3241 tgagtatagc aggatagaag acagattatt tctgaaactt caaaggtatc ttcttgtcac
     3301 gaactattct ttaattgaat cggttacact aaactagtcc cattatttaa aaacaaactt
     3361 taaatatgat tcacttcact ttttaagttt attaaatata gagtgattag gagaaataag
     3421 acgacctaat gataaaatca ttcctattgt atagcaaaca acaataaggt gtaaataaaa
     3481 gtaaattgat acctctgata tactcgtcaa acatcctatt ataataaaag caattaaggt
     3541 aggaataaga atagtacact taataaataa ccaaaattgc ttaacaactt gataccatgt
     3601 gagcacttct gtcgtttgtc tttgtttgta gacaaaaacg aataaatcaa ggataaaact
     3661 ccaacagata agttgtatat ttttcattaa tggtaaatcg tagaaactac ctatactaaa
     3721 tagaatcact gcagaaataa taacaatacc taaaaatctt aatatattac gcatatcttc
     3781 acctcctcta ctctatcggt agcaaatata accgaacaaa ttgttattcc tgttatcaga
     3841 gacagttata acttttttaa ccaattttca atacgcattt tgcttttaac tctttagtct
     3901 ctaaagttaa atatcatacg tactgatatt gtataagtca tccattttcc ccttaaatgt
     3961 gattgtatta taactcagta gaattaatct taacttctgg tatttttttc cataccaagt
     4021 atctataacg caaatataga taaacaataa gtataaaata aataaagatc acaataaggg
     4081 atatacctcc tagtacagca gcccaaatag gataagggtg cctaaagttg taggctacac
     4141 tcaagatgat aagggtaaaa gggacaatgt aacctcccag cttttcaaca ttttctagaa
     4201 atatcatctt tctatcagat aggttaaaaa taatatctaa aattttcata ccactcctct
     4261 actacttata catcagtgat agatagtcat ttatatattt agtgaggcca gataatattt
     4321 gtagcagcac cagctgctcc acacaaaata taaccttgcc aagggataaa tacgcctgtc
     4381 tgagcacaaa ctgtcactcc ctgcgcagca cctgccatcc ctgtcaaaaa gatatttgct
     4441 ccgtcttttc cgcctccatt tatgatagcc agaccatttt tttagcactt ccatattttt
     4501 gaagtctttt tcattttaat ttcactcttt tgtattttaa aaagctaaga gtgatatcta
     4561 aattcattgg cataatcact attttctttt atcgataaaa acaaataatg acgctaccaa
     4621 agctacgata gataacatgt ttaccgattt ttctgaaagg gataattgat tgaaaattaa
     4681 aaacactaaa tatggtatag atttatcaac atttttttat gaatggcatc aacaatgatt
     4741 aaaaacatta tagacgcatt cataataata aaaatcgtat agatcattta atttgctgta
     4801 tatcccaaat atgctccacc aactgtaaat cctacaacac atccacctgt caatactgga
     4861 tgtggcagtt tacaaaattt caatccagaa gctaagcctc caagaccacc aattacagca
     4921 tctcctggag cgtttcctcc acttatacta gccatgtcat taattgttaa ttccttcatt
     4981 gttgtcatcc tttccttcct ctagaatccg cctgtagctc ctgttacacc agcccaaaga
     5041 gtaatagctg ttttagctcc aggctacacc gcaaactgga aaacctatag cagctccagc
     5101 actacctgca gcgataaccc cactgacatt tgcctgccaa ttattcttac caccctcaac
     5161 cttggcaagc ttttctatag tcatttggtg aaatgtttcc agtttcttta tctccatatc
     5221 gaataatttc ttttcataat tcttaaattt atctcgttgc aacctcgact agtacagaat
     5281 atcataaaga acagtatcat ggtttcaaaa tcttaaaagg ctgtttttca tcatgaaatg
     5341 tctttttatc ctctctacac ttacgctgtg acaatagtta attaattaag aagtttaaat
     5401 gataccattc atgcttttat aaataccttc taagattttt gatggtagtg taaaatatct
     5461 tgtgtaaaca cacaaaaagg aataaatcct gtatagtaga gttgcgacac ttactagaaa
     5521 gagatttatt cccatgactc agtttaccac agaattactt aacttcctag cacaaaaaca
     5581 agatattgat gaattctttc gctcctcttt agaaatagct atgaatgacc tcttgcaagt
     5641 tgaactatca gcttttcttg gttatgaaac ttatgaaaaa gaaggttaca acacaggtaa
     5701 tagccgtaat gggacttatt ctcgtcagtt tgaaacaaag tatggcttag tcaatttaat
     5761 catcccaaga gatcgaaacg gcgagttttc accagtttta ttaccatctt atgctagacg
     5821 agaggaccac ttggaagaga tagtgatcaa actctatcag actggcgtta cgacacgcga
     5881 aatcagtgac attattaagc gcatgtatgg tgaccacttg tttctgagtg gaagcctcgt
     5941 tacaaaaagg ttatgacatc acttgaaaca acggaaaatc tcctaacttt ctatcaattt
     6001 ccacattaca tttggtctag tatttactct acaaacttga ttgaatcact caataaagaa
     6061 atcaagcgac aaagcaagaa ggagggtggt ttttccaaat gaagaagcct tagaacgatg
     6121 tcttgtaagt attttcgaag actaaaatac ttacaagaca tcgttctctt ttaggatata
     6181 tgatatctat ctatattaac tattacctca agagatactt tctaaaaact caaattatta
     6241 gtgggaaaaa cttagattat aaatacaaat gtcaggttta ttttatcata aatcatactt
     6301 ttttaaatct gagcaagtat atgaccttct aaacgaaata cgtatgcgtt atcctcatac
     6361 tcatattatt ctaatcggaa atcatattaa ttacgaggaa atatttaaaa atcattatcg
     6421 agtttttggt gttattgaca caacaagtca taaatcatta aggtccattc gagaccaaat
     6481 tcaactttac ttagacgaac tttacaattc gaataatgga taggcacttc atcaattctt
     6541 ttaggaaatg attcttgtca cccctcccaa gtctattgtc acagttaggt tcaacttgcc
     6601 taagtcatgc atggctaggt ggcaattcgt cataactcta aaacaaatgt ttgatgtctg
     6661 ctagtatcaa ttggaaagaa aatcctgtta acatttaaca aaattattca gacttaatca
     6721 gaaaaattgg aagcaaaaaa agagagcata agctctcgaa tatcgtcttt caaaaatttt
     6781 tttaacaagc caatctccaa aaatactcca actgtcctag agagttctta atgccccata
     6841 gcaaagctat cactaaaata ttcctctaac atcaggcgat gtttggctga gacactctct
     6901 aactctgcta tctcctcatg agagaaaaat tgtaacttga gcgtttcctc attatgaaag
     6961 ttatcaatag ccatatcaga aacagccgtt agttcataga taaagacaat agtctgaaca
     7021 gcatcaccgt tgggataaac ttcctcaaaa tgagtgtaaa cattcagtaa gcgaacagct
     7081 tcaacctcaa ttccggtttc ctctaagaac tcacgtttgc aagtctctaa agagctctca
     7141 cccagttcca tagtgccacc aggaatagcc caggttttct tatcaccacg caactgcatc
     7201 agcaccttgc cgtcatcatc ggtcaaaatc ccaccagcaa agttaagaat aatcttatca
     7261 tgcccaactt tggagcggat ataagaaatg tagtcttgtg gcatatgatc tcgctttact
     7321 atgttagtat tctttctcat tatagccaaa atcagctaaa tcaagctttt tatcccgcca
     7381 gtttttcttg actttgaccc acgtttctaa gtaaaccttg tcacctaaca tgagttcaat
     7441 atcacgacgt gccatttttc cgattttttt gagcatggct ccttgtttac cgatgataat
     7501 acctttttgg ctatcgcgct ccaccataat ggttgcacgg atatgcactt tgtcggtttc
     7561 ctcatctcgc ttcatggatt cgaccacaac cgctacggaa tgtggaacct cttgctgggt
     7621 gaggtgtaac actttctcac gaaccatttc tgacacaagg aaacgttctg gatgatcagt
     7681 aatctgatct tctggaaaat attggaaacc ttcttcaaga ttatcagtta ataacttgat
     7741 tagggttggc acattattac cttctagggc agaaattggg accacttctt taaaatccat
     7801 ttggctacgg aagtcatcaa tctgttccaa aagttggtct ggatgaacct tatcaatttt
     7861 gttgatgact aaaataacag gaatcttagc tgcctttagc cgctcgataa tcatgtcgtc
     7921 acccttacca cgcttttcat cggcaggaac catgaaaaga actgtctcaa cttcacgtaa
     7981 agtactataa gcagattcaa ccataaaatc accaagggcg gtctttggtt tatgaatacc
     8041 tggcgtatca ataaagacaa tttgttctgt ttctgtggtg taaatgccca taattttatt
     8101 acgggtggtc tgagccttgt cactcatgat ggcaattttt tgccccatga cgtgatttaa
     8161 aaaggtggat ttgccaacat ttggacgacc aagaatggct acaaagcctg atttaaacat
     8221 agataattac gggccttaac ggtagggctt cagatcaagc ttgtcactaa gcttaaccgt
     8281 cttgtccttt ccttattctc ctatgccatc atcacatagg atagcccatt cctttctttt
     8341 taatgaacga aaatattcca aattttagga ataaaaataa taagtcctgt caagacagca
     8401 tagccagaaa tcatcaaaac agcacctgct gccatatcct tggcattttt agctagcata
     8461 gaaaagtgat agtcactagc caaatcaact acattttcaa tagcagaatt gacaatttct
     8521 aaggtaatga ccaaaaaaat ggctagaagt aaaaataacc attcgatggc tgaaatgcta
     8581 aaaaataaac cagcaacaca agctaaacag gcagacaaaa ggtggcttct gagattgcgt
     8641 tcttccttaa aagctgtgaa aacccctgtc aaggcaaact ccaagcttga ggtaatggtt
     8701 ctgtttttcc atttccgttt ggtggtatta ttgtcgtgta aggccataag cagtcaaaat
     8761 ctcttcctgt aaggtaaaca tctctttttc ttcttcaagg gtgtagtgat catacccatt
     8821 aatatgtaag aatccatgaa cagctaaaaa tcccatttca cgctcaaaag agtgcccata
     8881 ttcttgggac tgctcacgcg ccttgtcaat agaaataaac aattcgccaa tataagcatc
     8941 aaactctgcc atcatctctg ccaaactagg atcagcagcc aaatcctctt gactaaataa
     9001 aataggcgtt tctggcttat attctagaga aatcacatct gttggacgat cagtatctcg
     9061 gtattctaag ttgagttcat gacttctttc attggtcaca aaggtcacag acatttcttt
     9121 ttcttctttg tccgtctttt gagcagcaaa attgagcaaa tcaagtgttt gctccataat
     9181 ctcttgcgaa acttgtcccg tttcgtcaat catctcgata tacataaggt taattgttag
     9241 ggaagggctg cccacctcat ttggtatgtt gggcacccat tattatctga cagataattt
     9301 cccttatcct ttcaatcagc atttgttatt tacccattat accacaagcc ccttaaaaat
     9361 gggaatgcta ggtaaatcag ggcctatttg gttagaattg cctccacacg tttttgcaag
     9421 ataggtaata aatcctgctc aaaccagggg ttcttagcta accacaattg gtttcttgga
     9481 gaaggatgaa caagtggaaa atagtctggc aagtaatcct caaagtgacg gactgtttca
     9541 gttaaggttt tgtaagcctt gttaccaagg taaaagtcct gagcatagcg accaaccaaa
     9601 atcgtcaatt caacttctgg catcaatgct cttaatggcg ggtgccactt agccgcaaaa
     9661 ccttctctag gtggtaagtc accggatttt cccttaccag gataatagaa atccataggg
     9721 ataatgccaa acaatcctga gtggtaaaag gtgtcattgt ccacacctag ccagtctcgt
     9781 aagcggatgc cactacgatc attccagtaa agtttggttt cttgagctac tattccagga
     9841 gcttggccaa caataataat cctagctgtt tgaggagcat catataaagg aaaaatacct
     9901 ctttcggtat aggagagatt agcctcatct gccataatag ctttggttaa atcatccata
     9961 gaatcctcct taaaaaggcc atattaaaca tcatcgtaaa aatcttagtg ctcttgaccg
    10021 atcacagggt attttgtcaa gcctggctgg taatcagctt caacgtcact agccttaggt
    10081 tttagtaagt ccttcatctc ctctgaggac gtttcataag ccttgataat atcagccaca
    10141 acaggatgtc ggacaacatc tttagcagag aaatagacaa aatcaatctg tttaatgcct
    10201 tgtaatttct gagtagc
//
