LOCUS       NZ_CAAHKK010000019     11553 bp    DNA     linear   CON 11-MAY-2024
DEFINITION  Streptococcus pyogenes strain A920, whole genome shotgun sequence.
ACCESSION   NZ_CAAHKK010000019
VERSION     NZ_CAAHKK010000019.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2019) WTSI, Pathogen Informatics, Wellcome Trust
            Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900983635.1-RS_2024_05_11
            Annotation Date                   :: 05/11/2024 02:49:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,652
            Genes (coding)                    :: 1,595
            CDSs (with protein)               :: 1,595
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 36 of 57
            Pseudo Genes (incomplete)         :: 21 of 57
            Pseudo Genes (internal stop)      :: 23 of 57
            Pseudo Genes (multiple problems)  :: 19 of 57
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:58:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 11553
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CAAHKK010000019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11553
                     /db_xref="taxon:1314"
                     /isolation_source="NA"
                     /mol_type="genomic DNA"
                     /note="contig: ERS1106251SCcontig000019"
                     /organism="Streptococcus pyogenes"
                     /serovar="EMM112.2"
                     /strain="A920"
     region          1..11553
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /tool="antismash"
     cand_cluster    1..11553
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11553
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3773:6557](+)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3774..6557
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     gene            51..566
                     /locus_tag="E9649_RS08745"
                     /old_locus_tag="SAMEA3919117_01711"
     CDS             51..566
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022534.4"
                     /locus_tag="E9649_RS08745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_01711"
                     /product="helical hairpin domain-containing protein"
                     /protein_id="WP_136022160.1"
                     /transl_table=11
                     /translation="MSFKTDCNTQSFVYFYSIHPLPYRKRVTLETLKEKVEKINLLMEI
                     HTPNQTFLETKDQLVQEIATLDMKIEEIQEKIATLNKMAEVFINLESDNKEGKRLARYN
                     LTKTNIPLTIKVDQVEREIEQLQISLTERLDEYEGKVSELEEFVQMLNTLELPLTDRDH
                     LDRNRLAF"
     gene            complement(782..2143)
                     /locus_tag="E9649_RS08750"
                     /old_locus_tag="SAMEA3919117_01712"
     CDS             complement(782..2143)
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677788.1"
                     /locus_tag="E9649_RS08750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_01712"
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="WP_109821250.1"
                     /transl_table=11
                     /translation="MNIFKKNILKFILSFFFIIIIDVILLITATNYIRSQQSASTIIET
                     VSSEITLSSNGKYNVSSKAKQLIDDKKLWIMIIDKDSGKEKFNINKPQIIENQFDFADA
                     IRFSRFYLKDYPVFTQIKDNDIYIIAFPKDSIIRYSSNYFELNRVQIFPILILSIIFAN
                     CLFCLFLYIYSVTFLNRNIKPIINAITKLPNGINTQVKSVRELDKLTLAINSANQQLRE
                     NEEFKENWISGIAHDIKTPLSVIVSNTSLAIEKTNDDEFLKHLKPTLVESHYIQNLLND
                     LNIFARLTNGNLKLNHEIVDIIPFFKEIIIQIINQEIWEDFNFEFIPDSKLLGKKMYIE
                     KALISRVVHNLIYNSVLHNHSGCDIKIILEYLPEDCFSITIRDNGIGTSPERLKNINKI
                     EEFNFDMSGVRRSGMGLKISKQIIDLHDGNMIITSTQGQYFQTVITLPIESLNT"
     gene            complement(2161..2859)
                     /locus_tag="E9649_RS08755"
                     /old_locus_tag="SAMEA3919117_01713"
     CDS             complement(2161..2859)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294154.1"
                     /locus_tag="E9649_RS08755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_01713"
                     /product="response regulator transcription factor"
                     /protein_id="WP_136047957.1"
                     /transl_table=11
                     /translation="MNFESQLLNKRILIVDDDVALSKSIKEVLISRDFKNISCAYSISE
                     GIDIFSVSKIDLIILDVMLPDGEGHLLAKYIRKYSDVPILFLTAKNNPDDEIRGLESGG
                     DDYVTKPFLPKSLTYRIIALLRRAYKDESELIELTNCTIDLNNASIEKNGQHLSLTPTE
                     IQILRKLFSNKNYIASTESICDTVWGLESLGYEKSLMVHIRNIREKIEITPSKPNHLIT
                     VKGLGYKLVI"
     gene            3057..3212
                     /locus_tag="E9649_RS08760"
                     /old_locus_tag="SAMEA3919117_01714"
     CDS             3057..3212
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) leader_d"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410306.1"
                     /locus_tag="E9649_RS08760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_01714"
                     /product="lacticin 481 family lantibiotic"
                     /protein_id="WP_023612114.1"
                     /sec_met_domain="L_biotic_A (E-value: 7.1e-30, bitscore:
                     92.5, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="leader_d (E-value: 3.7e-08, bitscore:
                     23.1, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKNNEVINSIQEVSLEELDQIIGAGKNGVFKTISHECHLNTWAF
                     LATCCS"
     CDS_motif       3057..3131
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MEKNNEVINSIQEVSLEELDQIIGA"
                     /locus_tag="E9649_RS08760_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       3132..3212
                     /prepeptide="core"
                     /locus_tag="E9649_RS08760_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="5.20"
                     /molecular_weight="2778.2"
                     /monoisotopic_mass="2776.3"
                     /core_sequence="GKNGVFKTISHECHLNTWAFLATCCS"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEKNNEVINSIQEVSLEELDQIIGA"
                     /leader_location="[3056:3131](+)"
                     /alternative_weights="2796.2"
                     /alternative_weights="2814.2"
     gene            3243..3398
                     /locus_tag="E9649_RS08765"
                     /old_locus_tag="SAMEA3919117_01715"
     CDS             3243..3398
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) leader_d"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410306.1"
                     /locus_tag="E9649_RS08765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_01715"
                     /product="lacticin 481 family lantibiotic"
                     /protein_id="WP_085613746.1"
                     /sec_met_domain="L_biotic_A (E-value: 2.5e-29, bitscore:
                     90.7, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="leader_d (E-value: 3.7e-08, bitscore:
                     23.1, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKEHEIINSIQEVSLEELDQIIGAGKNGVFKTISHECHLNTWAF
                     LATCCS"
     CDS_motif       3243..3317
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MTKEHEIINSIQEVSLEELDQIIGA"
                     /locus_tag="E9649_RS08765_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       3318..3398
                     /prepeptide="core"
                     /locus_tag="E9649_RS08765_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="5.20"
                     /molecular_weight="2778.2"
                     /monoisotopic_mass="2776.3"
                     /core_sequence="GKNGVFKTISHECHLNTWAFLATCCS"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MTKEHEIINSIQEVSLEELDQIIGA"
                     /leader_location="[3242:3317](+)"
                     /alternative_weights="2796.2"
                     /alternative_weights="2814.2"
     gene            3598..3753
                     /locus_tag="E9649_RS08770"
                     /old_locus_tag="SAMEA3919117_01716"
     CDS             3598..3753
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) leader_d"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002268661.1"
                     /locus_tag="E9649_RS08770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_01716"
                     /product="lacticin 481 family lantibiotic"
                     /protein_id="WP_023612118.1"
                     /sec_met_domain="L_biotic_A (E-value: 8.5e-27, bitscore:
                     82.6, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="leader_d (E-value: 1.9e-07, bitscore:
                     20.9, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEEKMCLGALNALQEFQIEELDNLLGGRGHGVNTISAECRWNSLQ
                     AIFTCC"
     CDS_motif       3598..3678
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MEEKMCLGALNALQEFQIEELDNLLGG"
                     /locus_tag="E9649_RS08770_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       3679..3753
                     /prepeptide="core"
                     /locus_tag="E9649_RS08770_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="3.80"
                     /molecular_weight="2594.9"
                     /monoisotopic_mass="2593.2"
                     /core_sequence="RGHGVNTISAECRWNSLQAIFTCC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEEKMCLGALNALQEFQIEELDNLLGG"
                     /leader_location="[3597:3678](+)"
                     /alternative_weights="2613.0"
     gene            3774..6557
                     /locus_tag="E9649_RS08775"
                     /old_locus_tag="SAMEA3919117_01717"
     CDS             3774..6557
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303301.1"
                     /locus_tag="E9649_RS08775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_01717"
                     /product="type 2 lanthipeptide synthetase LanM family
                     protein"
                     /protein_id="WP_136021017.1"
                     /sec_met_domain="DUF4135 (E-value: 7.2e-81, bitscore:
                     261.8, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 1.6e-69, bitscore:
                     224.0, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNPKELLYSQFDRFPKVVIENNFPELLNESSELIKDVEDEISDYY
                     RSTLIYLINEKRIEKNLIGDSPESRYEYFNNVLCQNGLIFEEIDRRFPSINQRVMSTIK
                     KRLELINFVKERFTLDFKELRETGYIYSEAQTPKISEVKIKITGDIHNGCGVCILSYEE
                     QKVVFKKKSSNPNVLLHELNIEVGKFLQKDIDFIPDFLDKGEYFWEKFVSSSPLRTEED
                     AKEFYRRMGYLLSYSYILNISDLHFENLISTSFSPKLVDVETVFSVSPYQTVANNESTL
                     EIINNSRNSILSTGLLPVSEAGKVFGGDTSGVLGGTLIGEAKIVINHNRDDIHVEKQKF
                     KTENQDHLPYFIDSKGMKEFLNAEDYVEYIKEGFREVSYFFMNSQDFLKKLYIKHNDIK
                     TRILFRNTRDYSLVRQLLVSPVYCEQSEILFETMANKLSEQNSRSLCLSEKKQLLNMDI
                     PYFYSNIDSCDIKDENMIIWNLESSALSEAINKLEKLSEEIINEQIELIEFSIKTPKAL
                     YSTELQEAYQKFEKVSSSENIIKTGIDTLVDIILENESNSLKDDSTNWLTLKVTDYDAF
                     ELVPMDDSLYEGLSGIAISLSEAYDFLDSGRQRRVKECLKRIFSVLSNSYMKLPNHSFF
                     VGKLGIYSALKRISVVTGQEIQNSIMNYNNLKYTLDVDVLSADFLSSFPNEITALRNSD
                     IKIDNLTQALDKLKELAIVQKDFISWDKLESNNVSLAHGNLGVEIALLYLAGKLESPEA
                     LNLFHKAKMFDKHQKLENGWIDKRNSSTSANWCHGSTGVLVARLAQLKLDDEYSLLSHS
                     ERIELENDMKHAAKQILEIGFDMTNFSLCHGTSGNLLALTYYQSYLTGADSEKLKEILD
                     REYRKLHSFGLENGWMCSFNTKYNVYGLMTGVSGILFSTVKYMKGDDSLDVLIPNF"
     gene            6580..8652
                     /locus_tag="E9649_RS08780"
                     /old_locus_tag="SAMEA3919117_01718"
     CDS             6580..8652
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002268663.1"
                     /locus_tag="E9649_RS08780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_01718"
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="WP_023612120.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 7.8e-41, bitscore:
                     128.7, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIILQNNEEDCLLACYTMLLNDLGHKVPLYEIYDKDTLPADGLN
                     VSYLLSLSDRFGVKINAYHASFDELLKVYGEKKQRMILHWNNDHFVVLEKITSNKTVIV
                     DPAIGRIKYSRDEFLTHYSETMVSVNKRNNFHPQTYKKIFWKYFKQTLQLKPIALFLLS
                     LLFIQVSVLLFSVILRQMLAEDFKFGISLFLLGAVLIFQLLGYFIKNGALDRYNTDFDK
                     YYSKELFQRLLQKPLLYFRNHLSGGVSEKISFKSTLRDNVTLKIIPSCVSLISAIIIFA
                     YLMTISIKLSVILILMIASYSIISTLLYHRQNEYNQTYLQYLIDFNSELQTDLDDIDYI
                     KIMRREKTVFHSWMEQNNQVTKKYSQILKIENLSQLIGTIFNYISLSSIIVIAVYYKDY
                     INLSLPDLLVYQTSISLLISAIEQVKGAVFEVIRLGVYAEKQSDLLKDTSPIVVPSSQS
                     DDYLIKTKNLNFSYGNKPIYSDVNLTISKGEKIAIVGKSGSGKSTLLLMLAGMLRYDGS
                     LEYGIEKFKDNLRVVLQNMTPRKGTVLENLEWTLDDVTPLYQVLNDTTADEVIARLPNK
                     IHSRLLKQGKNLSGGQIQKLLIAKSLLKENSIIFWDEAFSNLDEQSKNKIYDNVLQNNR
                     YSDRTMLIVSHHLDIVNYVDSIIFIDNETGKVIKDTHEHLMETNVNYTNFITSKN"
     gene            8764..9672
                     /locus_tag="E9649_RS08785"
                     /old_locus_tag="SAMEA3919117_01719"
     CDS             8764..9672
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263495.1"
                     /locus_tag="E9649_RS08785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_01719"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_085613744.1"
                     /transl_table=11
                     /translation="MKNAIELRKLSKVYQDITTVDLEKITVREGEIYGFLGPNGAGKTT
                     TMKMILSLVSPTLGEILINGEDIKGNHAYLSVIGSMIEEPSYYPNLTGYENLLVFQKMV
                     GFDESNIWPTLALVGLAEENNRKKLVKAYSLGMKQRLALAFALVKKPKILLLDEPTNGL
                     DPAGIHEIRELIVKLAKEKGLTVFISSHILSEIEHIADRVGIINHGRLVYEGEIEAIKS
                     NTWIEIGGDFSQNNIVQSLINYGQVEVLGASASHVKFKNIDNDKLADVVTYLIENDYRI
                     FRVVRESENLEDIFLSLTKEV"
     gene            9674..10438
                     /locus_tag="E9649_RS08790"
                     /old_locus_tag="SAMEA3919117_01720"
     CDS             9674..10438
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598724.1"
                     /locus_tag="E9649_RS08790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_01720"
                     /product="ABC transporter permease"
                     /protein_id="WP_085634204.1"
                     /transl_table=11
                     /translation="MIKAIQIELLKSKRTKSFMISFIMMLVAVTWSLLAASRSIDIPKL
                     RTIGLFFNNLQANSIFLPIATCLFVSRIVGNEKEGSTFKLQEANNTNLIIIFTRKLIFT
                     NSVFLLLNIFQVLIVYLNVLRYDIQVPIWTLILQVVGLTMSSFTLITLFLYLSIILEKQ
                     GILLGIGFLSGFFGMITSQTLKWINLIFPFGGSSFLALYRIKILNSSDSLDYIFSWDKS
                     VPLNYTLYLIYCVMIYSVVKYLLKKKKGDNNE"
     gene            10431..11168
                     /locus_tag="E9649_RS08795"
                     /old_locus_tag="SAMEA3919117_01721"
     CDS             10431..11168
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568302.1"
                     /locus_tag="E9649_RS08795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_01721"
                     /product="ABC transporter permease"
                     /protein_id="WP_136047958.1"
                     /transl_table=11
                     /translation="MNDYFKAEWLKTRKGAILAVGIIFLVLSSFIGLANFFLNYEVLIE
                     EQMSRVLWGQLTFYNSQLLFPAMLAIFSGMIILPEFERKTFEMLRANQVSVKKMLLSKF
                     LLALLLITVIQVLLFVIYLVTLKLSQIPFVAKDLLLFLRATVLSVIGSSSAVMIHSYIM
                     AKTKNFSKSVGIAAIGSFCGFIFIMLGGVINQFFPYSQPMIGIRSRALVDMSISEFVIF
                     IVVNALYSFIFFKLTLRTLEKNG"
     CDS             complement(11282..>11549)
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /locus_tag="E9649_RS08800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_01722"
                     /product="integrase core domain-containing protein"
                     /protein_id="WP_168389189.1"
                     /transl_table=11
                     /translation="GSQFKARELRKIIDVNTIMHSFSKPGYPYDNAVTEALFKYLKHRQ
                     INRKKYQNIKQVQLDCFEYIENFYNNYNPHTANLGLTPNQKEEN"
     gene            complement(11282..>11553)
                     /locus_tag="E9649_RS08800"
                     /old_locus_tag="SAMEA3919117_01722"
ORIGIN
        1 attcttcatc gtagcgttta actgttgaca taggttgtcc tttcgttttt gtgtctttta
       61 aaacagattg taacacacaa agttttgtct acttttatag tatacatcca cttccatatc
      121 gaaaacgagt aacacttgaa acattgaaag agaaggttga aaagataaat ctactaatgg
      181 aaattcatac tcctaaccaa acctttcttg aaaccaagga ccagctggtc caagaaatag
      241 ccacactaga catgaagata gaggaaatcc aagaaaaaat cgccacctta aacaagatgg
      301 cggaagtgtt tatcaatcta gaaagtgata ataaagaggg taaaaggcta gctaggtata
      361 acctgactaa gacgaatatt ccattaacta ttaaagttga ccaagtagag cgagaaatag
      421 aacagttaca aatttcttta acagaacgtc tagatgaata cgagggaaag gttagtgaac
      481 ttgaggaatt tgttcaaatg cttaatactc ttgaacttcc tcttactgat agagatcatc
      541 tagataggaa tagacttgct ttttaataga tcttagtgat ttatttgcag tagtgtctat
      601 cacaccaaat actaaataat gatttttaaa aattttttca tagttgatat tatctccgat
      661 aataatattt gcaatcgtga agaaagagcc taaaaaataa atattctgaa taacagaaag
      721 ctaaagtcat tattttttac ttatgtttcg gattttaatc tctgctctat caaaaatttt
      781 gttaagtgtt tagtgattca atagggaggg taattactgt ttggaagtac tgcccttgtg
      841 tacttgtaat tatcatgttt ccatcatgta aatcaataat ttgtttagaa attttcaatc
      901 ccataccact ccttcgaacg cctgacatat caaaattaaa ttcttcaatc ttattgatat
      961 tttttagtct ttcaggagaa gttcctatac cattatcacg aatagtaatc gaaaaacaat
     1021 cttcaggtag atattctaaa atgattttaa tgtcgcaacc agaatgatta tgaagtactg
     1081 aattataaat taggttatga acaactctag aaatgagagc tttttcaata tacattttct
     1141 tacctaatag tttagaatca ggaatgaatt caaaattgaa gtcttcccaa atttcttgat
     1201 tgataatctg aatgattatt tctttgaaga aagggattat atcaacaatc tcatggttta
     1261 gttttaagtt tccgtttgtt aatcgagcaa atatatttaa atcgtttagg agattttgaa
     1321 tgtagtgact ttcaactaag gtgggtttca aatgtttaag gaattcgtca tcatttgttt
     1381 tttcaatggc taaggaagtg ttagatacaa ttactgaaag tggtgtcttt atatcatgtg
     1441 ctattccaga aatccaattc tccttgaatt cttcgttttc acgaagttgc tgatttgctg
     1501 agttaatagc aagtgttaat ttatctaatt ctcgtacaga tttgacctga gtattaattc
     1561 catttggtaa tttagtaatt gcgttaatga taggtttaat gttacgattt aaaaaagtaa
     1621 cagaataaat gtaaagaaat aggcagaaca aacagtttgc aaatatgata gacaaaataa
     1681 ggattggaaa aatttgaact ctatttagtt cgaaataatt actactataa cgtattatac
     1741 tgtcttttgg aaaagcaatg atgtaaatat cattatcttt tatttgcgta aatacaggat
     1801 agtcttttag gtaaaatcta gaaaatcgta tagcatctgc aaaatcgaat tgattttcta
     1861 taatctgagg tttattaata ttaaattttt cttttccact atctttgtca ataatcatta
     1921 tccataactt tttatcatca atgagttgtt tagctttaga gctaacatta tatttaccat
     1981 tagaagatag agtgatttca gatgagacag tttcgattat agtagatgca gactgctgac
     2041 ttcggatata attggtagca gttattaaca gtatgacgtc aattattata ataaagaaaa
     2101 atgataggat aaattttagt atatttttct tgaaaatatt catgctatat tccttatagt
     2161 ttatataact agtttataac caagcccttt tacagtaata agatgatttg gcttagatgg
     2221 agttatctcg attttttctc tgatattacg aatatgtacc ataagtgatt tttcgtagcc
     2281 aaggctctca agtccccaaa cggtatcaca aatcgattca gttgatgcaa tataattttt
     2341 atttgaaaat aattttcgta ggatttgtat ttctgttgga gtgagtgaca aatgttgacc
     2401 atttttttca atacttgcat tgtttaagtc tatggtacaa tttgttagct caattagttc
     2461 cgattcatct ttataagccc ttctcaacag tgcaataata cgatatgtta aagatttggg
     2521 aagaaatggc ttcgtaacat aatcatcccc tccggattca agtcctcgaa tttcatcatc
     2581 aggattgttt ttggctgtta aaaagagaat tggcacatct gaatatttcc taatgtattt
     2641 tgcaagtaag tgcccttctc catctggtag cataacatct aaaataatga gatcaatttt
     2701 agaaactgag aagatatcaa ttccttcgct tatactataa gcacagctga tattcttgaa
     2761 atcccgactg ataagtacct ctttaattga tttgctaagt gcgacatcat cgtcgacaat
     2821 caatatgcgt ttattcaata attgagactc aaaattcata caatacctcc taaagtgtaa
     2881 taaaattata ccataaagat tatagatata agagaataag taggacttgt gttgtataat
     2941 cttaaaatta aatttatgtt ttctttattc tgtcttaaag ttatcatctt aaaataaggt
     3001 tgtaacaaag aggaaggcac ctatccttct gaagaaagtt aaatgaggta tagaaaatgg
     3061 aaaaaaataa tgaagtaatc aactctattc aagaagttag tcttgaagaa ctcgatcaaa
     3121 ttatcggtgc tggaaaaaat ggtgtgttta aaacaatttc tcatgagtgt catttgaata
     3181 catgggcatt ccttgctact tgttgctcat aatattagat aaaatataag gagaaaatcg
     3241 ttatgaccaa agaacatgaa attattaact ctattcaaga agttagtctt gaggagttgg
     3301 accaaattat cggtgctggg aaaaacggtg tatttaagac aatttctcat gagtgtcatt
     3361 tgaatacatg ggcattcctt gctacttgtt gttcataaaa ctctactgtt caggtgtctt
     3421 tgatgttaca taaaaaaaga gggggacctc tttttttatt tgcattgaca taggtagaaa
     3481 atttaattta actttagttt tccttaatat tgtctttatg taacaaattt aaaatcaatg
     3541 tataaagaaa acaaatttcg agttctactc gatgtcatat aaatgaggta aagaattatg
     3601 gaagaaaaaa tgtgcctagg tgctttgaat gcgttacaag aatttcagat tgaggaattg
     3661 gataaccttt tgggaggaag agggcatggt gtcaatacaa tttcagctga atgtcgttgg
     3721 aatagtttac aggcaatttt tacttgttgt taatatgttt aggagtaaag aatatgaatc
     3781 caaaagaact gttatatagc caatttgata gattcccaaa agtagttatt gaaaataatt
     3841 tcccagaatt gctaaatgaa agtagtgagc tgattaagga tgttgaagat gaaatttctg
     3901 attattatcg ttccactctt atttacctaa taaatgaaaa aagaattgag aaaaacctaa
     3961 ttggagattc tccagagtca cgctatgaat actttaataa tgtactatgt cagaatggct
     4021 taatttttga agaaattgat agacgtttcc cttctattaa tcagcgtgta atgagtacta
     4081 tcaagaaacg tttggaatta attaattttg taaaggaaag atttacttta gatttcaaag
     4141 agttgagaga gacaggctat atctatagtg aagctcagac tccaaaaatt agtgaagtca
     4201 aaatcaaaat aactggagat attcacaatg gttgtggagt ctgcattttg agttatgaag
     4261 aacaaaaagt agtttttaag aaaaaatcat caaatcccaa tgttctcttg catgaactga
     4321 atatagaagt tggtaaattc ttacagaaag atatagactt tatccctgat tttttagata
     4381 aaggagagta tttttgggaa aaatttgtat caagttctcc gttaagaacc gaggaggatg
     4441 caaaggaatt ttatcgtagg atgggttact tattatcata ttcttacata ttgaatatct
     4501 ctgatttgca ttttgaaaat ctgatttcta caagcttttc tcctaagtta gttgatgttg
     4561 agacagtttt ttcagttagc ccctatcaaa ctgttgcaaa taacgagtca acattggaga
     4621 taattaataa tagtagaaat tcgatattat caacaggttt gcttcctgtt tctgaagctg
     4681 gtaaagtttt tggaggagat actagtggtg ttctaggagg caccttaatt ggagaggcaa
     4741 aaatcgtcat caatcataat cgagatgata ttcatgttga aaaacaaaag tttaaaacag
     4801 aaaaccagga tcatttgcct tactttattg attcgaaggg aatgaaagaa tttcttaatg
     4861 ctgaagatta tgttgaatat atcaaagaag ggttccgtga agtcagctac tttttcatga
     4921 attcacaaga ttttttaaag aagctttata ttaagcataa tgatataaag actagaattt
     4981 tgttcagaaa tacaagagat tatagcttag tgcgccaatt gctagtttct cctgtttatt
     5041 gtgagcaatc cgagatttta tttgaaacaa tggccaataa attgtctgaa caaaatagtc
     5101 gtagtttatg tctatcagaa aagaaacagc ttttaaatat ggatatccct tatttttact
     5161 caaatattga tagttgtgat ataaaggatg aaaacatgat tatttggaac cttgaaagtt
     5221 ctgctttaag cgaagcaatc aataaattag aaaaattgag tgaagaaatc ataaatgagc
     5281 aaattgaatt aattgagttt tctatcaaaa ctcctaaagc tttatatagt acggaacttc
     5341 aagaggcata tcaaaaattt gagaaggtta gtagcagtga aaatataata aaaactggta
     5401 ttgatactct agttgatatt attttggaaa atgaaagcaa ctctctaaaa gacgatagta
     5461 caaactggct caccttgaaa gttacagatt atgatgcttt tgagttagtt ccgatggatg
     5521 actctcttta tgaaggtttg tctggaatag caatttcttt atcagaggct tatgattttc
     5581 tagactcagg aagacaaaga agggttaaag aatgtctaaa acgaattttt tctgtattat
     5641 ctaattccta tatgaaatta ccaaatcatt ctttttttgt ggggaagctg ggaatttact
     5701 cagctttaaa acgaattagt gttgttacag gacaagagat tcaaaattct atcatgaatt
     5761 ataataatct caaatataca ttggatgtag atgttttaag tgctgatttt ctatcaagtt
     5821 ttcctaatga aatcactgct ttgagaaatt cggatattaa gatagataat cttactcagg
     5881 cactagacaa acttaaagag ttggcaattg ttcaaaagga ttttattagt tgggataagt
     5941 tagagtcgaa taatgttagt cttgcacatg gtaatttagg agttgaaata gcgttacttt
     6001 atttagctgg aaagttagaa agtcctgagg cactaaattt atttcataaa gcaaaaatgt
     6061 ttgataaaca tcaaaaattg gaaaacggct ggatagacaa aagaaattcc tcgactagtg
     6121 ccaactggtg tcatggttca acaggagtat tagttgcaag gttagcacag ttaaaactag
     6181 atgatgaata tagccttctt tctcattctg aacggataga attagagaat gatatgaagc
     6241 atgctgctaa acagattctt gaaattggtt ttgatatgac aaacttttca ctttgccatg
     6301 gaaccagtgg aaatttgctg gcacttactt actaccaatc atatttaaca ggagctgatt
     6361 cagagaagtt aaaagaaatt ttggatagag agtatagaaa actgcattct tttggcctgg
     6421 aaaacggctg gatgtgtagt tttaatacaa aatataatgt ttatgggtta atgacaggtg
     6481 tgtcaggaat attattttct acagtaaagt acatgaaggg tgatgattcc ctagatgtgt
     6541 tgattcccaa tttctaagag atagaaagga atatgagata tgaatattat tttgcaaaac
     6601 aatgaggagg attgtctctt agcttgttat acaatgttac ttaatgattt aggacataaa
     6661 gttccattat atgaaattta tgataaagat acattgcctg cagatggtct aaatgtctca
     6721 tatcttttgt cactaagtga cagatttggg gtgaaaataa atgcttatca tgcctctttt
     6781 gatgaattac taaaagtata cggagaaaag aagcaaagaa tgattcttca ttggaataat
     6841 gatcattttg tggttcttga aaaaattacg tcaaataaaa cagttatagt tgacccagcc
     6901 attggtcgta ttaagtatag ccgtgatgaa tttttaactc actactcgga aactatggtg
     6961 tcagttaaca aaagaaataa ttttcacccc caaacatata agaaaatctt ttggaagtat
     7021 ttcaaacaga ccttacaatt aaaaccgatt gcattattct tgttgtcact cctgtttatt
     7081 caagttagtg ttttactgtt ctcagtcatt ttaagacaaa tgttagctga agatttcaag
     7141 tttggtatca gcttattcct tttaggtgca gtattaattt ttcaattatt aggttatttt
     7201 ataaagaatg gtgcattaga tagatataat acagactttg ataaatatta cagtaaagag
     7261 ttgtttcaaa gattattaca aaaaccttta ctttatttta gaaatcatct aagtggggga
     7321 gtatctgaaa aaattagttt taaatcaaca cttagggaca atgttacttt aaaaattatt
     7381 ccatcatgtg taagtttgat ttcagctatt attatctttg catatctgat gactatatct
     7441 attaagttgt cggtgatatt aatcttgatg attgcttcat atagtataat ctctactcta
     7501 ttgtatcata ggcaaaacga gtataatcag acatatttac agtacttaat tgattttaac
     7561 tcagagttac aaacagatct tgatgacatt gactatataa aaataatgag aagagagaaa
     7621 actgttttcc attcctggat ggagcaaaat aatcaagtca ctaagaagta ttcgcagatt
     7681 cttaaaattg aaaatctttc acaacttatt ggaactattt ttaactacat tagtctatcc
     7741 tcaataattg ttatcgcagt atattataag gattatatta atttatccct tcctgatcta
     7801 ttagtttatc aaactagtat ttctttattg atatcagcaa ttgaacaagt aaaaggagca
     7861 gtatttgaag taattcgatt gggtgtatat gcagaaaaac aaagtgattt attaaaagat
     7921 acttcaccta tcgttgtacc tagttcacag tcagacgatt atttaatcaa aactaaaaat
     7981 ttgaactttt cttatggtaa taaaccaatc tatagtgatg tgaatttaac aatcagtaaa
     8041 ggagaaaaga ttgccattgt aggtaaatct ggtagcggga agtcaacttt actattaatg
     8101 ttagcaggta tgctacgcta tgatggatct cttgaatatg gcatcgaaaa attcaaagac
     8161 aatctaagag ttgttcttca aaatatgaca cctagaaagg ggactgtctt agaaaactta
     8221 gagtggactt tagatgatgt aactccactt taccaagtat tgaatgatac aacagctgat
     8281 gaagttatag cacgattacc aaataagatt cactctagac tcttaaaaca aggaaaaaat
     8341 ttatcaggtg gacaaatcca gaagttactc atagcaaaat cacttttaaa agaaaattca
     8401 attatttttt gggacgaggc tttcagtaat ctagatgaac aaagtaagaa caaaatttac
     8461 gataatgttt tacaaaacaa tagatattca gacagaacta tgttaatcgt tagtcatcat
     8521 ttagatattg tcaattatgt tgattctatt atttttattg ataatgaaac tggtaaggtt
     8581 attaaagata ctcatgaaca tcttatggaa acaaatgtta actatacaaa tttcattact
     8641 tccaaaaatt aagagaatgt ttctcttttc ttaatgttat ctttaagttg cttagataca
     8701 ataatgttgt aatagaagga aaggaagaga ctcattgaag ggtcttatta tggggttatt
     8761 aacatgaaaa atgctattga attaagaaaa ctttctaagg tctatcagga tattactacg
     8821 gtagatttag aaaagattac ggttcgtgaa ggcgagattt acggttttct tggaccgaat
     8881 ggtgcaggaa aaacaacaac aatgaaaatg attttgtcac tagtttctcc aacactagga
     8941 gaaattctta taaatggtga agatataaag gggaaccacg cttatttgag tgtaatcggt
     9001 tcaatgattg aggaaccctc ctattatcca aatttaacag gttacgagaa tctactagtc
     9061 tttcaaaaaa tggtaggttt tgatgaaagt aatatctggc cgactttggc attggttggt
     9121 ttagcagagg aaaataaccg aaaaaaactt gtgaaagcct actctttagg catgaagcaa
     9181 cggttagcct tggcttttgc tctggttaaa aaaccaaaaa tcttgttatt agatgaacct
     9241 actaatggac ttgatccggc tggaattcat gaaattcgcg agttaattgt aaaattagct
     9301 aaggagaaag ggctaacagt atttatctca agtcatattt tatccgaaat tgagcatata
     9361 gctgaccgag taggcattat aaatcatggg agactggttt atgaagggga aattgaagca
     9421 ataaaatcaa atacttggat tgagattggc ggggatttct cgcaaaataa tattgttcaa
     9481 agtttaatta attacggaca ggttgaagtg ctaggtgctt cagcaagtca tgttaagttt
     9541 aaaaatattg ataatgacaa attagcagat gtagtgactt atttaattga aaatgactat
     9601 cggattttcc gagttgttag ggaaagcgag aatttagaag acattttcct ctctttaact
     9661 aaggaggttt gaaatgataa aagcaataca gattgaatta ttaaaaagta agcgtacgaa
     9721 atcttttatg atttctttca ttatgatgtt agtagcagtg acgtggtcat tgttagcagc
     9781 ctcaagaagt attgatattc ctaaattacg gactatcgga ttgttcttta ataatttaca
     9841 agctaattct atttttttac ctattgcaac ttgcctattt gtttcacgta ttgttggtaa
     9901 tgaaaaagaa ggtagtacat ttaaacttca agaagcaaat aatacaaatc taattataat
     9961 atttactcgc aaattgattt tcacaaattc tgtctttcta cttttgaata ttttccaagt
    10021 cttgattgtt tatctaaatg tactaagata tgatatccaa gttccaattt ggactctgat
    10081 tttacaagta gtaggtttaa cgatgtccag ttttacttta atcactctct ttctttattt
    10141 atcaataatt ttagaaaaac aagggattct tttaggaatt ggatttttat caggtttttt
    10201 cggtatgatt acctcccaga ctttaaagtg gattaacctt atttttccgt ttggaggatc
    10261 gagtttttta gcactatata gaataaagat tttaaattcc agtgatagtt tagattatat
    10321 tttttcttgg gacaaaagcg tgcctttaaa ttatacttta taccttattt actgcgtaat
    10381 gatctatagc gtagttaagt atttactgaa aaagaagaaa ggggacaata atgaatgatt
    10441 atttcaaagc ggagtggcta aaaactcgca aaggagctat tttagcagtt gggattatat
    10501 ttcttgttct atctagtttc attggtttgg caaatttctt tttgaattat gaggtactca
    10561 ttgaggaaca aatgagtaga gtactctggg gacagttaac tttctataat agtcagttgc
    10621 ttttcccagc tatgttagca atattctcag gcatgattat tctacctgaa tttgagcgaa
    10681 aaacatttga aatgttacgt gcaaatcaag tatctgttaa aaagatgctt cttagtaagt
    10741 tcttgttagc tttgctactt ataacagtta tacaagtatt attatttgtt atatatcttg
    10801 taacactgaa attgagccag attccgtttg tagcaaaaga tttactttta tttttaagag
    10861 caactgtgct atctgttatt ggttctagct ctgctgtaat gattcatagt tatatcatgg
    10921 ctaaaacaaa aaatttttct aaatcagtcg ggattgcagc tataggttca ttttgtggat
    10981 tcatatttat tatgttggga ggtgtcatta atcaattttt cccttattcc cagccaatga
    11041 taggtatacg tagtagagcg ttagtagata tgagcatttc ggagttcgta atatttatag
    11101 ttgttaatgc tctatatagt tttatttttt tcaaattgac attacgaact ttagagaaga
    11161 atggataaag tggattttta tacattacag aagtaactaa atttatagaa ggaaaaggtg
    11221 agagcatgtg gactaatgtc aaataagtag acagaaaacc gtgttatttt attacgttaa
    11281 actaattttc ttctttctga ttaggggtta gtcctagatt agccgtatgt gggttgtaat
    11341 tgttataaaa attctcaatg tattcaaagc agtctaattg aacctgtttg atattttgat
    11401 acttttttcg gttgatttgt ctatgcttta aatacttgaa aagtgcttca gttacggcat
    11461 tatcataagg atatccaggt ttagaaaaag aatgcatgat agtgttgaca tcaattattt
    11521 ttctcaattc cctggcctta aattgtgacc ctt
//
