LOCUS       NZ_CAAHKK010000007     11883 bp    DNA     linear   CON 11-MAY-2024
DEFINITION  Streptococcus pyogenes strain A920, whole genome shotgun sequence.
ACCESSION   NZ_CAAHKK010000007
VERSION     NZ_CAAHKK010000007.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2019) WTSI, Pathogen Informatics, Wellcome Trust
            Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900983635.1-RS_2024_05_11
            Annotation Date                   :: 05/11/2024 02:49:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,652
            Genes (coding)                    :: 1,595
            CDSs (with protein)               :: 1,595
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 36 of 57
            Pseudo Genes (incomplete)         :: 21 of 57
            Pseudo Genes (internal stop)      :: 23 of 57
            Pseudo Genes (multiple problems)  :: 19 of 57
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:58:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 28423
            Orig. end                         :: 40306
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CAAHKK010000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11883
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11883
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11883
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6883](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6883
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(617..630)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.93"
                     /tool="antismash"
     gene            913..1488
                     /locus_tag="E9649_RS04455"
                     /old_locus_tag="SAMEA3919117_00874"
     CDS             913..1488
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /locus_tag="E9649_RS04455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00874"
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="WP_009880369.1"
                     /transl_table=11
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIVA
                     QETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFAAK
                     WHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPSPRN
                     QLWLAKNPWFEQDLLPILQKRVEAILTK"
     gene            1647..2144
                     /gene="ybeY"
                     /locus_tag="E9649_RS04460"
                     /old_locus_tag="SAMEA3919117_00875"
     CDS             1647..2144
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ybeY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /locus_tag="E9649_RS04460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00875"
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="WP_002985748.1"
                     /transl_table=11
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVTN
                     ERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGELFI
                     SIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAYGLT
                     RQ"
     gene            2125..2532
                     /locus_tag="E9649_RS04465"
                     /old_locus_tag="SAMEA3919117_00876"
     CDS             2125..2532
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /locus_tag="E9649_RS04465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00876"
                     /product="diacylglycerol kinase"
                     /protein_id="WP_002985746.1"
                     /transl_table=11
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLLS
                     ACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNAKD
                     MAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     misc_feature    complement(2584..2599)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.57"
                     /tool="antismash"
     gene            2652..3548
                     /gene="era"
                     /locus_tag="E9649_RS04470"
                     /old_locus_tag="SAMEA3919117_00877"
     CDS             2652..3548
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="era"
                     /gene_functions="other (smcogs) SMCOG1227: ribosome
                     biogenesis GTP-binding protein YsxC"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /locus_tag="E9649_RS04470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00877"
                     /product="GTPase Era"
                     /protein_id="WP_002985743.1"
                     /transl_table=11
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIMG
                     IYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDDMI
                     IERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTLIKL
                     LTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKRDEET
                     DKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVKVKKNW
                     RDKKLDLADFGYNEKEY"
     gene            3568..4044
                     /locus_tag="E9649_RS04475"
                     /old_locus_tag="SAMEA3919117_00878"
     CDS             3568..4044
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /locus_tag="E9649_RS04475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00878"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_002985741.1"
                     /transl_table=11
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKTW
                     AIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFIYE
                     LTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
     gene            complement(4350..4604)
                     /locus_tag="E9649_RS09030"
                     /old_locus_tag="SAMEA3919117_00879"
     CDS             complement(4350..4604)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990779.1"
                     /locus_tag="E9649_RS09030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00879"
                     /product="hypothetical protein"
                     /protein_id="WP_011106868.1"
                     /transl_table=11
                     /translation="MSGLFYHKSYFFKSEQVYDLLNEIRMRYPHAHIILIGNHINYEEI
                     FKNHYRVFGVIDTTSHKSLRSIRDQIQLYLDELYNSNNG"
     gene            5001..5235
                     /locus_tag="E9649_RS04485"
                     /pseudo=""
     CDS             5001..5235
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994504.1"
                     /locus_tag="E9649_RS04485"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="Blp family class II bacteriocin"
                     /pseudo=""
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.4e-10,
                     bitscore: 34.3, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKFAEEIQKEELFHIIGGYSATDCKNHLIGGITSGAIAGRYGYF
                     RCWRSCRCVCRCTCRCYCGWINLRWWYVI"
     misc_feature    5030..5044
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.12"
                     /tool="antismash"
     gene            5250..5432
                     /locus_tag="E9649_RS04490"
                     /old_locus_tag="SAMEA3919117_00881"
     CDS             5250..5432
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987564.1"
                     /locus_tag="E9649_RS04490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00881"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_002987564.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.8e-08,
                     bitscore: 27.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNFLELQFEELVNISGGKGNIGSAIGGCLGGMLIAAAGGPITGG
                     AAAFVCVASGIAAYQ"
     gene            complement(5652..6338)
                     /locus_tag="E9649_RS04495"
                     /pseudo=""
     CDS             complement(5652..6338)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /locus_tag="E9649_RS04495"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTQFTTELLNFLAQKQDIDEFFRSSLEIAMNDLLQVELSAFLGYE
                     PYEKEGCNTGNSRNGTYSRQFETKYGLVNLIIPRDRNGEFSPVLLPSYARREDHLEEIV
                     IKLYQTGVTTREISDIIKRMYGHHLFLSGSLVTKRL"
     gene            6656..6883
                     /locus_tag="E9649_RS04500"
                     /old_locus_tag="SAMEA3919117_00884"
     CDS             6656..6883
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016754.1"
                     /locus_tag="E9649_RS04500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00884"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_002992018.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 4.7e-18,
                     bitscore: 58.2, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIKKLETFHQMTIEKLAKVEGGKNNWQANVSGVIAAGSAGAAIG
                     FPVCGVACGYIGAKTAITLWAGVTGATGGF"
     gene            6896..7096
                     /locus_tag="E9649_RS04505"
                     /old_locus_tag="SAMEA3919117_00885"
     CDS             6896..7096
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /locus_tag="E9649_RS04505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00885"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_003060803.1"
                     /transl_table=11
                     /translation="MTTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHPV
                     LTGGCVVGFTVGGAYLGYTAN"
     gene            7412..7585
                     /locus_tag="E9649_RS09160"
     CDS             7412..7585
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073350.1"
                     /locus_tag="E9649_RS09160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bacteriocin"
                     /protein_id="WP_322348456.1"
                     /transl_table=11
                     /translation="MAIINGGGKDGANIFLTGMAGAAEGVIVCAQTGVFIPWQGYILCG
                     AAGAATNIIWPH"
     gene            7635..7916
                     /locus_tag="E9649_RS04520"
                     /old_locus_tag="SAMEA3919117_00886"
     CDS             7635..7916
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528350.1"
                     /locus_tag="E9649_RS04520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00886"
                     /product="hypothetical protein"
                     /protein_id="WP_111676660.1"
                     /transl_table=11
                     /translation="MKTLDIIFNLSDRKMIFLENVENLGGYIVPFTLIILSVAYNFRHP
                     YPIWAAVLGGISLIVIFIYFILIVYLYLRYRYLVWKKIPEIKINFTEL"
     gene            8110..8511
                     /locus_tag="E9649_RS04525"
                     /old_locus_tag="SAMEA3919117_00887"
     CDS             8110..8511
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /locus_tag="E9649_RS04525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00887"
                     /product="hypothetical protein"
                     /protein_id="WP_047235194.1"
                     /transl_table=11
                     /translation="MRNILRFLGIIIISAVILFSIGSFYDLPLMKNIQLICWSFILDLF
                     VFVYKQRQTTEVLTWYQVVKQFWLFIKCTILIPTLIAFIIIIGCLTSISEVSIYFYLHL
                     TVVCYTIGMILSLGRLISPNHSIFNKLKK"
     gene            8762..9833
                     /locus_tag="E9649_RS04530"
                     /old_locus_tag="SAMEA3919117_00888"
                     /pseudo=""
     CDS             8762..9833
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /locus_tag="E9649_RS04530"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00888"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MMMLRDVKVKTSSLLVGCAATLLVSLSSTVAADSVHSSDRRFGVG
                     YKEETTSQFFSDQPSQVVLYRGEANTVRFTYTNQMYLMKDIRIALDGSGKSLTAQTVPG
                     MSHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMHNTMYKGTVFLV
                     QPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGGSGALPDNIKVSIN
                     PKGGAGHDYLWGWLYGYYSSSSFMEKRLRKRAY"
     gene            9926..10324
                     /locus_tag="E9649_RS04535"
                     /old_locus_tag="SAMEA3919117_00889"
     CDS             9926..10324
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /locus_tag="E9649_RS04535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00889"
                     /product="histidine kinase"
                     /protein_id="WP_168389169.1"
                     /transl_table=11
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNTPFLLPKKVLSLED
                     DAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQYQTGIT
                     YGDDTLADLAHHLLHSDGVIPSLNDKSA"
     gene            complement(10672..10941)
                     /gene="stcA"
                     /locus_tag="E9649_RS04540"
     CDS             complement(10672..10941)
                     /codon_start=1
                     /gene="stcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /locus_tag="E9649_RS04540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="quorum-sensing system protein StcA"
                     /protein_id="WP_010921974.1"
                     /transl_table=11
                     /translation="MTKKQFYLILAISILCFILAVSPKPPILKHTIERKRTAMTIGHSK
                     QNPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(11463..11681)
                     /locus_tag="E9649_RS09165"
     CDS             complement(11463..11681)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285423.1"
                     /locus_tag="E9649_RS09165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_148852125.1"
                     /transl_table=11
                     /translation="MMCLNKTLTIKLSAPLTSNDLVPLSTNEVIIMEPLLHLLPKVVPV
                     LIHLVDKANVSWLTVILQLPILLKTIW"
ORIGIN
        1 aaagccctac tggttctagt gggtcgtggc atggtggtca acacatcaga tgtggtgaca
       61 gctttatcta tggcagaatc tcatcagatt gaccaattta tggcgcttta tgaagaagaa
      121 atcattaaag ataactatgg taaggcaata cgagtcaaaa cattaggtca aaaaacctat
      181 gttgatagtg tcaaacgtca tgatgtggtt tttggtgtag gacctgcagg gacagggaaa
      241 accttcttag ctgttacgct tgcagtcact gctctgaaaa gaggacaggt aaagcgtatt
      301 attttgacgc gtcctgcggt agaagctggt gaaagcctag gttttttacc aggcgatttg
      361 aaagaaaagg tggatcctta cctcagaccg gtttacgatg ccctttatca cattttaggc
      421 aaggaacaaa ccacacgcct gatggaacga gatgtgattg agattgctcc gttagcttat
      481 atgcggggtc gtactctgga tgacgctttt gttatcttag atgaggctca aaacacgacc
      541 atcatgcaaa tgaagatgtt tttgacccgt cttggtttta attctaaaat gattgtcaat
      601 ggagatacga gtcagattga tctccctcga aatgtcaagt ctggtttgat tgatgctact
      661 cagaaattac aaggcattaa acagattgat tttgtctatt tctctgctaa agatgttgtc
      721 cgacatcctg ttgtggctga tattatcaag gcttatgaaa cgtcctcaga ggagatgaag
      781 gacttactaa aacctaaggc tagtgacgtt gaagctgatt accagccagg cttgacaaaa
      841 taccctgtga tcggtcaaga gcactaagac ctttacgatg atgtttaata tggccttttt
      901 aaggaggatt ctatggatga tttaaccaaa gctattatgg cagatgaggc taatctctcc
      961 tataccgaaa gaggtatttt tcctttatat gatgctcctc aaacagctag gattattatt
     1021 gttggccaag ctcctggaat agtagctcaa gaaaccaaac tttactggaa tgatcgtagt
     1081 ggcatccgct tacgagactg gctaggtgtg gacaatgaca ccttttacca ctcaggattg
     1141 tttggcatta tccctatgga tttctattat cctggtaagg gaaaatccgg tgacttacca
     1201 cctagagaag gttttgcggc taagtggcac ccgccattaa gagcattgat gccagaagtt
     1261 gaattgacga ttttggttgg tcgctatgct caggactttt accttggtaa caaggcttac
     1321 aaaaccttaa ctgaaacagt ccgtcacttt gaggattact tgccagacta ttttccactt
     1381 gttcatcctt ctccaagaaa ccaattgtgg ttagctaaga acccctggtt tgagcaggat
     1441 ttattaccta tcttgcaaaa acgtgtggag gcaattctaa ccaaataggc cctgatttat
     1501 ctagcattcc catttttaag gggcttgtgg tataatgggt aaataacaaa tgctgattga
     1561 aaggataagg gaaattatct gtcagataat aatgggtgcc caacatacca aatgaggtgg
     1621 gcagcctttc cctaacaatt aaccttatgt atatcgagat gattgacgaa acgggacaag
     1681 tttcgcaaga gattatggag caaacacttg atttgctcaa ttttgctgct caaaagacgg
     1741 gcaaagaaga aaaagaaatg tctgtgacct ttgtgaccaa tgaaagaagt catgaactca
     1801 acttagaata ccgagatact gatcgtccaa cagatgtgat ttctctagaa tataagccag
     1861 aaacgcctat tttatttagt caagaggatt tggctgctga tcctagtttg gcagagatga
     1921 tggcagagtt tgatgcttat attggcgaat tgtttatttc tattgacaag gcgcgtgagc
     1981 agtcccaaga atatgggcac tcttttgagc gtgaaatggg atttttagct gttcatggat
     2041 tcttacatat taatgggtat gatcactaca cccttgaaga agaaaaagag atgtttacct
     2101 tacaggaaga gattttgact gcttatggcc ttacacgaca ataataccac caaacggaaa
     2161 tggaaaaaca gaaccattac ctcaagcttg gagtttgcct tgacaggggt tttcacagct
     2221 tttaaggaag aacgcaatct cagaagtcac cttttgtctg cctgtttagc ttgtgttgct
     2281 ggtttatttt ttagcatttc agccatcgaa tggttatttt tacttctagc catttttttg
     2341 gtcattacct tagaaattgt caattctgct attgaaaatg tagttgattt ggctagtgac
     2401 tatcactttt ctatgctagc taaaaatgcc aaggatatgg cagcaggtgc tgttttgatg
     2461 atttctggct atgctgtctt gacaggactt attattttta ttcctaaaat ttggaatatt
     2521 ttcgttcatt aaaaagaaag gaatgggcta tcctatgtga tgatggcata ggagaataag
     2581 gaaaggacaa gacggttaag cttagtgaca agcttgatct gaagccctac cgttaaggcc
     2641 cgtaattatc tatgtttaaa tcaggctttg tagccattct tggtcgtcca aatgttggca
     2701 aatccacctt tttaaatcac gtcatggggc aaaaaattgc catcatgagt gacaaggctc
     2761 agaccacccg taataaaatt atgggcattt acaccacaga aacagaacaa attgtcttta
     2821 ttgatacgcc aggtattcat aaaccaaaga ccgcccttgg tgattttatg gttgaatctg
     2881 cttatagtac tttacgtgaa gttgagacgg ttcttttcat ggttcctgcc gatgaaaagc
     2941 gtggtaaggg tgacgacatg attatcgagc ggctaaaagc agctaagatt cctgttattt
     3001 tagtcatcaa caaaattgat aaggttcatc cagaccaact tttggaacag attgatgact
     3061 tccgtagcca aatggatttt aaagaagtgg tcccaatttc tgccctagaa ggtaataatg
     3121 tgccaacttt gatcaagtta ttaactgata atcttgaaga aggtttccaa tattttccag
     3181 aagatcagat tactgatcat ccagaacgtt tccttgtgtc agaaatggtt cgtgagaagg
     3241 tactgcattt gacgcaacaa gaggttccac attccgtagc tgttgttgtc gaatccatga
     3301 agcgagatga agagactgat aaagtgcata tccgtgcaac cattatggtg gagcgcgata
     3361 gccaaaaagg catcatcatt ggaaaacaag gagccatgct caaaaaaatc ggaaaaatgg
     3421 cgcgccgtga tattgaactc atgttaggtg acaaggtcta cttagaaacg tgggtcaaag
     3481 tcaagaaaaa ctggcgggat aaaaagcttg accttgctga ctttggttat aatgagaaag
     3541 aatactaaca tagcaaagcg agatcatatg ccacaagact acatttctta tatccgctct
     3601 aaagttgggc atgataagat tattcttaac tttgctggtg ggattttgac caatgatgac
     3661 ggcaaggtgc tgatgcagtt gcgtggtgat aagaaaacct gggctattcc tggtggcact
     3721 atggaactgg gtgagagctc tttagagact tgcaaacgtg agttcttaga ggaaaccgga
     3781 attgaggttg aagctgttcg cttactgaat gtttacactc attttgagga agtttatccc
     3841 aacggtgatg ctgttcagac tattgtcttt atctatgaac taacggctgt ttctgatatg
     3901 gctattgata actttcataa tgaggaaacg ctcaagttac agtttttctc tcatgaggag
     3961 atagcagagt tagagagtgt ctcagccaaa catcgcctga tgttagagga atattttagt
     4021 gatagctttg ctatggggca ttaagaactc tctaggacag ttggagtatt tttggagatt
     4081 ggcttgttaa aaaaattttt gaaagacgat gtttcgagag cttatgctct ctttttttgc
     4141 ttccaatttt tctgattaag tctgaataat tttgttaaat gttaacagga ttttctttcc
     4201 aattgatact agcagacatc aaacatttgt tttagagtta tgacgaattg ccacctagcc
     4261 atgcatgact taggcaagtt gaacctaact gtggcaatag acttggaagg ggtgacaaga
     4321 atcatttcct aaaagaattg atgaagtgcc tatccattat tcgaattgta aagttcgtct
     4381 aagtaaagtt gaatttggtc tcgaatggat cttaatgatt tatgacttgt tgtgtcaata
     4441 acaccaaaaa ctcgataatg atttttaaat atttcctcgt aattaatatg atttccgatt
     4501 agaataatat gagcatgagg ataacgcata cgtatttcgt ttagaaggtc atatacttgc
     4561 tcagatttaa aaaagtatga tttatgataa aataaacctg acatttgtat ttataatcta
     4621 agtttttccc actaataatt tgagttttta gaaagtatct cttgaggtaa tagttaatat
     4681 agatagatat catatatcct aaaagagaac gatgtcttgt aagtattttc gaagactata
     4741 atattaagtt cggagctcgt attcataaag gattcggagt atgttttgac acacttgata
     4801 gcttatttga ctaatattca aaaactctac tcttgtgttt acacaacatt attgacagtc
     4861 tctaatatgt taaaaagttt atttgagatg tttatacaac cattcacgac atagaaaaaa
     4921 tttttataat aatatgctat atttagtttg tcaaagttgc aacaagatga atataacatt
     4981 ttagaaaggt ttttaaagtt atgataaaat ttgcagaaga aatacaaaaa gaagagttgt
     5041 tccatattat tggaggttat agtgctacag attgtaaaaa tcatttaatt ggtggtatta
     5101 cttcaggtgc tattgcaggt aggtatggct actttaggtg ttggaggagt tgcaggtgcg
     5161 tttgcaggtg cacatgtagg tgctattgcg ggtggattaa cttgcgttgg tggtatgtta
     5221 tttaatggaa aataattttg aggtgaaaaa tggataattt tttagaatta cagtttgaag
     5281 aattagtaaa tatttctggt ggaaaaggaa atattggatc tgctatcgga gggtgcttag
     5341 gtgggatgct tatagctgca gcaggaggtc caataactgg aggtgcggca gcatttgtat
     5401 gtgtagcatc tggaattgca gcttatcaat gatatgatta tagttataat actcggttta
     5461 atttgtctaa tactttccca aatttttttg aaggataaag aggtaagaga aaatgcaagt
     5521 aaagtaattt taattgtgat atttgtttat gtcttactaa gtactattac aaattaataa
     5581 tattaaaggg atatagatta attgagactg tcaataatgt tgtgtaaaca caagagtaga
     5641 gtttttgaat attagtcaaa taagctatca agtgtgtcaa aacatactcc gaatccttta
     5701 tgaatacgag ctccgaactt aatattatag tcttcgaaaa tacttacaag acatcgttct
     5761 aaggcttctt catttggaaa aaccaccctc cttcttgctt tgtcgcttga tttctttatt
     5821 gagtgattca atcaagtttg tagagtaaat actagaccaa atgtaatgtg gaaattgata
     5881 gaaagttagg agattttccg ttgtttcaag tgatgtcata acctttttgt aacgaggctt
     5941 ccactcagaa acaagtggtg accatacatg cgcttaataa tgtcactgat ttcgcgtgtc
     6001 gtaacgccag tctgatagag tttgatcact atctcttcca agtggtcctc tcgtctagca
     6061 taagatggta ataaaactgg tgaaaactcg ccgtttcgat ctcttgggat gattaaattg
     6121 actaagccat actttgtttc aaactgacga gaataagtcc cattacggct attacctgtg
     6181 ttgcaacctt ctttttcata aggttcataa ccaagaaaag ctgatagttc aacttgcaag
     6241 aggtcattca tagctatttc taaagaggag cgaaaaaatt catcaatatc ttgtttttgt
     6301 gctaggaagt taagtaattc tgtagtaaac tgagtaatgg gaataaatct ctttctagta
     6361 agtttcgcaa ctctactata caggatttat tcctttttgt gtgtttacac aagatatttt
     6421 acactaccat caaaaatctt aaaaggtatt tataaaagca tgaatggtat catttaaact
     6481 tcttaattaa ttaactattg tcacagcgta agtgtagaga ggataaaaag acatttcatg
     6541 atgaaaaaac ggccttttaa gattttgaag ccatgatact gttctttatg atatcctgta
     6601 ctagtcgagg ttgcaacgag acaaatttaa gaattatgaa aagaagttat ccgatatgga
     6661 gataaagaaa ctggaaacat ttcaccaaat gactatagaa aagcttgcca aggttgaggg
     6721 tggtaagaat aattggcagg caaatgtcag tggggttatc gctgcaggta gtgctggagc
     6781 tgctataggt tttccagttt gcggtgtagc ctgtggttat attggagcta aaacagctat
     6841 tactctttgg gctggtgtaa caggagctac aggcggcttc tagaggaaag aaaggatgac
     6901 aacaatgaag gaattaacaa ttaatgacat ggctagtata agtggaggaa acgctccagg
     6961 agatgctgta attggtggtc ttggaggctt agcttctgga ttgaaatttt gtaaactgcc
     7021 acatccagta ttgacaggtg gatgtgttgt aggatttaca gttggtggag catatttggg
     7081 atatacagca aattaaatga tctatacgat ttttattatt atgcatgcgt ctataatgtt
     7141 tttaatgatt gttgatgcca ttcataaaaa aatgttgata aatctatacc atatttagtg
     7201 tttttaattt tcaatcaatt atccctttcg gaaaaatcgg taaacatgtt atctatcgta
     7261 gctttggtag cgtcattatt tgtttttatc gataaaagaa aatagtgatt atgccaatga
     7321 atttagatat cactcttagc tttttaaaat acaaaagagt gaaattaaaa tgaaaaagac
     7381 ttcaaaaata tggaagtgct aaaaaaatgg tctggctatc ataaatggag gcggaaaaga
     7441 cggagcaaat atctttttga cagggatggc aggtgctgcg gaaggagtga tagtttgtgc
     7501 tcagacaggc gtatttatcc cttggcaagg ttatattttg tgtggagcag ctggtgctgc
     7561 tacaaatatt atctggcctc actaaatata taaatgacta tctatcactg atgtataagt
     7621 agtagaggag tggtatgaaa actttagata ttatttttaa cctatctgat agaaagatga
     7681 tatttctaga aaatgttgaa aatctgggag gttacattgt cccttttacc cttatcatct
     7741 tgagtgtagc ctacaacttt aggcaccctt atcctatttg ggctgctgta ctaggaggta
     7801 tatcccttat tgtgatcttt atttatttta tacttattgt ttatctatat ttgcgttata
     7861 gatacttggt atggaaaaaa ataccagaaa ttaagattaa ttttactgag ctataatgca
     7921 atcacattta aggggaaaat ggatgaccta tacaatatca gtacttatga tatttaactt
     7981 tagagactaa agagttaaaa gcaaaatgcg tattgaaaat tggttaaaaa agttataact
     8041 gtctctgata acaggaataa caatttgttc ggttatattt gctaccgata gagtagagga
     8101 ggtgaagata tgcgtaatat attaagattt ttaggtatta ttattatttc tgcagtgatc
     8161 ctatttagta taggtagttt ctacgattta ccattaatga aaaatataca acttatctgt
     8221 tggagtttta tccttgattt attcgttttt gtctacaaac aaagacaaac gacagaagtg
     8281 ctcacatggt atcaagttgt taagcaattt tggttattta ttaagtgtac tattcttatt
     8341 cctaccttaa ttgcttttat tataataata ggatgtttga cgagtatatc agaggtatca
     8401 atttactttt atttacacct tactgttgtt tgctatacaa taggaatgat tttatcatta
     8461 ggtcgtctta tttctcctaa tcactctata tttaataaac ttaaaaagtg aaatgaatca
     8521 tatttaaagt ttgtttttaa ataatgggac tagtttagtg taaccgattc aattaaagaa
     8581 tagttcgtga caagaagata cctttgaagt ttcagaaaca atctgttttc catcctgcta
     8641 tactcatctt tgccaaagct cgtcatttta tatgatttat atagaattac gagttttttg
     8701 ttagactaat atgagaaaat atagtatgac gaattcctaa ataaaaaata atgaaaggaa
     8761 catgatgatg ttaagagatg ttaaagttaa aacgagcagt ttattagttg gttgtgcggc
     8821 tactttacta gtcagtttga gtagtacagt tgcggcagat tcagtccatt cgtctgatag
     8881 acgttttgga gttggctata aggaagaaac aacctctcag tttttttcgg atcagccctc
     8941 ccaagtggtt ttgtatcgag gtgaggctaa cacggttagg tttacctata ccaatcagat
     9001 gtatctgatg aaagatattc gcattgcttt ggatggttct ggtaagtctt tgaccgctca
     9061 gactgttcct ggtatgagtc atgtttatga gggctttcaa acttctgcta gagggatttt
     9121 tacgatgtca ggagttcctg aaagcactgt tcccgttgct aaccctaatg tacaaaccaa
     9181 atatacaagg tatttcaaag tcattgatga tatgcataac acaatgtata aaggaactgt
     9241 ttttcttgtt caaccgcaag cttggaaata caccatgaaa tctgttgatc agttaccagt
     9301 agatgacttg aaccatattg gcgttgctgg tattgaacga atgacaactc tcattaaaaa
     9361 tgcgggtgcc cttttaacca caggaggtag tggagcctta ccggacaata ttaaagtatc
     9421 tattaatcca aagggggggg ctggccacga ttacttatgg ggctggctct acggatatta
     9481 ttcctccagc agttttatgg aaaaaaggct ccgtaaaaga gcctactgaa gccgatcaat
     9541 ctgtcggaac accgactcct ggtattcctg gtaaattcaa acgagaccag agccttaacg
     9601 agcatgaagc tatggtaaat gtcgaaccac tatctcatgt agtaaaagac aatataaagg
     9661 tcatagatga aaaatcaaca gggcggtttg agccttttag acctaatgaa gatgagaagg
     9721 agaagcctgc cagtgatgtt aaggtaagac cagcagaagt tggtagctgg ctagaaccag
     9781 cgacagctct tcctagtgtt gaaatgagcg ctgaggacag gttaaaaagt tagttaataa
     9841 tttgccggtt gctttagaca ggcaaacagg aaagggcttg gaggtttctt ctgaagtagc
     9901 tgtgagcgcc tcacagccgg aagaattgca tcgattgcct aaccgtttga tacctgagga
     9961 agacaataat aaactatcac gagtagccaa ggaagtcgtt actgagccaa ggctaaatac
    10021 cccctttttg ttacccaaga aggttctttc gttagaagat gatgctgcta tccttaatca
    10081 tttggaggaa gaccgtaatg tcaataagtt gttcctcgag ccagttgcgg ctgaagccaa
    10141 gagagaaatt aattttcatc caagtgacca gcaggtgact gctaaagtta aggagtcatc
    10201 tgattttcat cagaaacagt accaaacagg gattacttat ggagatgata ctctggcaga
    10261 tctggcgcat catttgcttc attcagatgg tgttattcct agtctaaatg ataagagtgc
    10321 ttaagaatta tgtgctagta agtctcgcta agaagaggag tgttacaaga taaataaaga
    10381 agaggtcagg gaactaccgg cctctttttg tccgaaatag cgagaatgac tctagcttac
    10441 ctgtatgtag gtccgtgcta cacatcttgt taatgatagg gagctgggat cagaaaataa
    10501 ttggtatatc gttttctcct aactttttaa gaaagtgcgg gcaaagtggg ctgttagggt
    10561 gatggggttg ctctttcatg atcagttttt tagcttgaag aaagcgtggt gatgagatta
    10621 gcttaaaaaa gaacactacc gaaatggtag tgtccactga ttttaggaga gctaagattg
    10681 gtcatctaac aggagtctaa ctgtcatgct aataacagat ttgatagcgc tgtccccact
    10741 tggaagaaat agagccttat ttttattttt tttgtgttta agggcttctt ttttgtgagg
    10801 gttttgtttt gagtggccta tggtcatcgc agtccttttc ctttcaatag tgtgtttaag
    10861 aattggtggt ttagggctga cggcaagaat aaaacacagt atgctaatag ctaagataag
    10921 ataaaactgt tttttggtca ttgtttaggc ctttctagtt gaaaaatgta gttgttgtta
    10981 gtttagcctt tttggtagct ggtatttaaa atagcattag ccaagtggtt gtactggcta
    11041 tctaaacggt actcagtatt cctcacgtct tttccagcaa ggaaatacct tgtggtgtat
    11101 gctgaatcat tccaggtagc atcaacgtag gaattaacac catgaatggt aacgatgttt
    11161 caagcatggt gctcattcaa tcctttttta tcacatacca agctgataag cctgcagctt
    11221 ctgccatatc tttaaacatg acggcataag cttgacaaac accacacttg ccacgataaa
    11281 gagctttagg agtatgattg ttgatgctgc ttacggtttt gcaattggtg ctaagttatt
    11341 ttgtggcgta agtgtattcg ttgattaaat ggttataaat aaccttagcc ttgtcgtagt
    11401 cactggtgat cggcttatct gtaatatttt tttgacaaag gttttatatt ccttatgaag
    11461 ggctaccaaa ttgtctttaa gagtataggt aactgtaata tcactgttaa ccatgatacg
    11521 ttggctttgt ctaccaagtg tataagtact ggtacgactt tgggaagtaa atgaagtaag
    11581 ggttccataa taatcacctc attcgtcgag agtggcacca ggtcgttgct ggttaatgga
    11641 gctgatagtt tgatagtaag tgttttgttc agacacatca taacggtatg cccctactgt
    11701 ttcatcagct ttgaccttgc tgtcaatggc agcaaggtca aagctgccgc taggtcgcta
    11761 acactctttt taatatctat tagttttcct gttacaaact aaatataagg atttcccaaa
    11821 agtggattgt ttttaagcga aaggttatcc accaacgata atcaggatat ccatgatcaa
    11881 tgt
//
