LOCUS       NZ_CAAHKK010000003     20873 bp    DNA     linear   CON 11-MAY-2024
DEFINITION  Streptococcus pyogenes strain A920, whole genome shotgun sequence.
ACCESSION   NZ_CAAHKK010000003
VERSION     NZ_CAAHKK010000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2019) WTSI, Pathogen Informatics, Wellcome Trust
            Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900983635.1-RS_2024_05_11
            Annotation Date                   :: 05/11/2024 02:49:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,652
            Genes (coding)                    :: 1,595
            CDSs (with protein)               :: 1,595
            Genes (RNA)                       :: 57
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 36 of 57
            Pseudo Genes (incomplete)         :: 21 of 57
            Pseudo Genes (internal stop)      :: 23 of 57
            Pseudo Genes (multiple problems)  :: 19 of 57
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:58:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 88957
            Orig. end                         :: 109830
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CAAHKK010000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20873
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="1"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20873
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20873
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10873](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10873
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    complement(765..785)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 25.95"
                     /tool="antismash"
     misc_feature    1659..1672
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.93"
                     /tool="antismash"
     gene            2046..2276
                     /locus_tag="E9649_RS02510"
                     /old_locus_tag="SAMEA3919117_00498"
     CDS             2046..2276
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105222.1"
                     /locus_tag="E9649_RS02510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00498"
                     /product="YkuJ family protein"
                     /protein_id="WP_002983885.1"
                     /transl_table=11
                     /translation="MESHLVRIINRLELMVTDGGNLKRNFERDGVVVAEVAFSNDPENG
                     PVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            2403..3089
                     /locus_tag="E9649_RS02515"
                     /old_locus_tag="SAMEA3919117_00499"
     CDS             2403..3089
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018000.1"
                     /locus_tag="E9649_RS02515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00499"
                     /product="amino acid ABC transporter permease"
                     /protein_id="WP_023610107.1"
                     /transl_table=11
                     /translation="MDLSFLPKYWAYFNYGVLVTIMISVSVVFFGTLIGVLVTLIKRSH
                     VKPLTWVVNLYVWIFRGTPMVVQIMIAFAWMHFNNMPTIGFGVLDLDFSRLLPGIIIIS
                     LNSGAYISEIVRAGIEAVPKGQLEAAYSLGIRPQNAMRYVILPQAFKNILPALGNEFIT
                     IIKDSALLQTIGVMELWNGAQSVVTATYSPISPLLVAAFYYLMVTTVMAQLLAVLECHM
                     AQGGNH"
     gene            3089..3823
                     /locus_tag="E9649_RS02520"
                     /old_locus_tag="SAMEA3919117_00500"
     CDS             3089..3823
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407690.1"
                     /locus_tag="E9649_RS02520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00500"
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="WP_010922488.1"
                     /transl_table=11
                     /translation="MTEAIISIKDLHKYYGHNEVLKGIDLDIMPGEVVVIIGPSGSGKS
                     TLLRTMNLLEVPTKGQIRFEGIDITDKKNDIFSMREKMGMVFQQFNLFPNMTILENITL
                     SPIKTKGMAKAEADKTALSLLDKVGLSEKAKAYPASLSGGQQQRIAIARGLAMDPDVLL
                     FDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAKEVADRVMFMDGGVIVEEG
                     SPNQLFDLTKEERTKDFLSRVL"
     gene            3972..5381
                     /locus_tag="E9649_RS02525"
                     /old_locus_tag="SAMEA3919117_00501"
     CDS             3972..5381
                     /EC_number="4.1.99.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989109.1"
                     /locus_tag="E9649_RS02525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00501"
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="WP_010922487.1"
                     /transl_table=11
                     /translation="MVSVMWFRRDLRIDDQKALAHAIASQSPTLCVFQFNKEQLNLLHS
                     RNQSSFITSVLAFRKLLKCEGIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGYGR
                     LRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKETPIK
                     VELSQGRWLNLETPDDVLRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHANRDF
                     PAQLGTSRLSPFLRIGAIGIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYVAYPDQ
                     KTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRMIVASFL
                     TKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVTQGKRFDP
                     KGEFIKAYLPQLEHVPEKYLHEPWKMPKNLQESVSCIIGTDYPQPIVDHAKQREQAIAK
                     YEWAKEKAKIE"
     misc_feature    complement(5497..5508)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.16"
                     /tool="antismash"
     misc_feature    5526..5541
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 22.0"
                     /tool="antismash"
     gene            5559..7253
                     /locus_tag="E9649_RS02530"
                     /old_locus_tag="SAMEA3919117_00502"
     CDS             5559..7253
                     /EC_number="5.4.2.-"
                     /GO_function="GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /locus_tag="E9649_RS02530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00502"
                     /product="phospho-sugar mutase"
                     /protein_id="WP_002989112.1"
                     /transl_table=11
                     /translation="MTYNEVYQEWLHNNDLSDDIKADLAAIKDNEAEIQDRFYKTLEFG
                     TAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAVKKGIAVSYDVRYQSRTFAEL
                     TCSIMAANGIKAYLYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQEGS
                     QILDDIADQIAQHMAALTQYQEIKQMPFEKALDSGLVTYIDESIEEAYKKEVLGLTIND
                     TDIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPNPEVPK
                     TFAYSEKLGKAVDADILIATDPDCDRVALEVKNAVGDYVFLNGNKIGALLSYYIFSQRF
                     DLGNLPANPVLVKSIVTGDLSRAIASHYGIETVETLTGFKNICGKANEYDVTKQKNYLF
                     GYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKKKGQNLLDVLQTIYATFGYYNERQI
                     ALELEGIEGQKRIARIMEDFRQTPIASVAEMALDKTIDFIDGYQDFPKQNCLKFYLDDG
                     SWYALRPSGTEPKIKFYLYTIGQTQENSATKLDAIEAACRTKINQVN"
     gene            7461..8315
                     /locus_tag="E9649_RS02535"
                     /old_locus_tag="SAMEA3919117_00503"
     CDS             7461..8315
                     /GO_function="GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /locus_tag="E9649_RS02535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00503"
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="WP_010922486.1"
                     /transl_table=11
                     /translation="MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDPA
                     SQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPNHI
                     NDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKHAVI
                     IGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQYIKD
                     GAIVIDVGMNRDDNGKLIGDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQSALRS
                     THK"
     gene            8468..9808
                     /gene="xseA"
                     /locus_tag="E9649_RS02540"
                     /old_locus_tag="SAMEA3919117_00504"
     CDS             8468..9808
                     /EC_number="3.1.11.6"
                     /GO_component="GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA]"
                     /GO_function="GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /GO_process="GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="xseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /locus_tag="E9649_RS02540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00504"
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="WP_136047702.1"
                     /transl_table=11
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQYF
                     SLKDESAVIQATMWVGVYKKLGFDLEEGMKINVIGRVQLYEPSGSYSIVIEKAEPDGIG
                     ALALQFEQLKKKLTAEGYFEQKHKQPLPQFVSKIGVITSPSGAVIRDIITTVSRRFPGV
                     EILLFPTKVQGDGAAQEVVANIRRANQREDLDLLIVGRGGGSIEDLWAFNEEIVVQAIF
                     ESQLPVISSVGHETDTTLADFVADRRAATPTAAAELATPITKTDLMSWIVERQNRSYQA
                     CLRRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSMTLMNTMKDRLSSAKENKVQ
                     LDHALANSQLQTKIERYQDRVATAKRLLMANMTNQYDSQLARFEKAQDALLSLDASRII
                     ARGYAMIEKNQALVASVSQITKGDQLTIKMRDGQLDVEVKDVKNENI"
     gene            9786..10001
                     /locus_tag="E9649_RS02545"
                     /old_locus_tag="SAMEA3919117_00505"
     CDS             9786..10001
                     /EC_number="3.1.11.6"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /locus_tag="E9649_RS02545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00505"
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="WP_002983901.1"
                     /transl_table=11
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKEL
                     QKTLQAAEKTLVKVMQADGTEVDMDD"
     gene            10001..10873
                     /locus_tag="E9649_RS02550"
                     /old_locus_tag="SAMEA3919117_00506"
     CDS             10001..10873
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /locus_tag="E9649_RS02550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00506"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_136047703.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.2e-52, bitscore:
                     171.8, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKLARIDEAIRRYYKTTSNGVSEELIDAILYSVDSGGKRIRPLI
                     LLEMIEGFGVSLQNAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFGEAT
                     AILAGDSLFLDPFGLIAQAELNSEVKVALIQELSLASGTFGMVGGQMLDMKGENQALSL
                     PQLLLIHLNKTGKLLTFPFKAAALVTEQAITVRQQLEQAGMLIGHAFQIRDDILDVTAS
                     FEDLGKTPKKDLFAEKATYPSLLGLEASYQLLTESLDQALTIFQTLESDVGFKPQIITK
                     LIEGLRLNA"
     gene            10866..11693
                     /locus_tag="E9649_RS02555"
                     /old_locus_tag="SAMEA3919117_00507"
     CDS             10866..11693
                     /EC_number="2.1.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /locus_tag="E9649_RS02555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00507"
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="WP_003056952.1"
                     /transl_table=11
                     /translation="MPKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPGD
                     KIDDGTELKLKGEKLKYVSRGGLKLEKGLHVFGVSVANQIGIDIGASTGGFTDVMLQDG
                     AKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFNEGQPVFASIDVSFISLSL
                     ILPALHNVLSDQGQVIALIKPQFEAGREQIGKKGIVKDKQIHEKVIQKVMDFASGYGFT
                     VKGLDFSPIQGGHGNIEFLAHLAKSQTPETLAPHLIQKVVAKAHKEFEKHEKE"
     gene            11680..12150
                     /locus_tag="E9649_RS02560"
                     /old_locus_tag="SAMEA3919117_00508"
     CDS             11680..12150
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /locus_tag="E9649_RS02560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00508"
                     /product="arginine repressor"
                     /protein_id="WP_002983907.1"
                     /transl_table=11
                     /translation="MKKSERLELIKKMVLTHPIETQHDLLRLLAEHGLELTQATISRDM
                     NEIGIVKIPSGSGRYIYGLSQDSGKKIVQGPRSIKSTILAVSDKTKGLEQHLYLKVVPG
                     NSKLIKRYLLADFSKAIFSLIADDDSLLLIAKSPSEADMIRQEILLWMQGIT"
     gene            12172..13833
                     /gene="recN"
                     /locus_tag="E9649_RS02565"
                     /old_locus_tag="SAMEA3919117_00509"
     CDS             12172..13833
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /locus_tag="E9649_RS02565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00509"
                     /product="DNA repair protein RecN"
                     /protein_id="WP_002983909.1"
                     /transl_table=11
                     /translation="MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNMM
                     LGARASTEVIRRGANKAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGRSV
                     SRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKENYQL
                     IFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDRLMNH
                     KQIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYYILEEV
                     SKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNVNDVLDYYDNIIKEYQLLTGD
                     DLSSGDLESELKALEKQLVAAATNLSERRHQLAEQLEAEIKAELKELYMEKADFKVHFT
                     TSKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKEDKTSIVFD
                     EVDTGVSGRVAQAIAQKIYKIGRHGQVLAISHLPQVIAIADYQYFISKESKEESTVSKV
                     RLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH"
     gene            14005..14961
                     /locus_tag="E9649_RS02570"
                     /old_locus_tag="SAMEA3919117_00510"
     CDS             14005..14961
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285633.1"
                     /locus_tag="E9649_RS02570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00510"
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_136047704.1"
                     /transl_table=11
                     /translation="MTSKKACLSSIIVLASLTCGNDTVSANHLSATGDKFDDCSTLVEK
                     DVAPKDELEMLAWSSSQTTDDADRDYEDFLDDDSFNDDSFISQNETDKMFENLTDDRLL
                     NELDESDESDESDEEVTIEPEQNVIMPSDDELFDLTDAVETRLTVSSAPHLEAELPKPH
                     LRSLSDTALRSGEIRGHLDNKLDALSVTATKLALTMAQKFDLTTHVYSIGESFSEVLAA
                     HYEDRKAESAFSKKKRFHLPIATPDVVIEELRRLVSSIGSSKEDVSVPYSRKLGMAVAK
                     RKIALPQTGEGFSYYPALLGLMILGLTPIMIPKKINN"
     gene            15189..16028
                     /locus_tag="E9649_RS02575"
                     /old_locus_tag="SAMEA3919117_00511"
     CDS             15189..16028
                     /GO_function="GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264715.1"
                     /locus_tag="E9649_RS02575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00511"
                     /product="DegV family protein"
                     /protein_id="WP_136047705.1"
                     /transl_table=11
                     /translation="MGTIKIVTDSSITIEPELIKALDITVVPLSVMIDSKLYSDNDLKE
                     EGHFLSLMKASKSLPKTSQPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTIEASRQG
                     AEIAEAPVTVLDSGFTDQAMKFQVVEAAKMAKAGASLNEILAVVQAIKSKTELYIGVST
                     LENLVKGGRIGRVTGVLSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLDSYLAKNSH
                     RPIAEIAISYAGEASLALTLKERIAAYYNHSISVLETGSIIQTHTGEGAFAVMVRYE"
     gene            16021..16863
                     /locus_tag="E9649_RS02580"
                     /old_locus_tag="SAMEA3919117_00512"
     CDS             16021..16863
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /locus_tag="E9649_RS02580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00512"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_002992620.1"
                     /transl_table=11
                     /translation="MNNRHLFSGIFFFVISLCLAFLLLNIIIPKSNSRLKKSDFLKKEQ
                     VAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQILDR
                     MIKQKQIQLSLKKADIMTLTVGGNDVMAVIRKNLADLQVSSFRKPARQYQKRLRQIIEL
                     ARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNTKTKEVVGEYDRVYFVPINDLL
                     YKGINGQEGIVHSSGDQTTIVNDALFTGDHFHPNNTGYQIMSNAVMEKIKKHEKKIKP"
     gene            16841..17428
                     /locus_tag="E9649_RS02585"
                     /old_locus_tag="SAMEA3919117_00513"
     CDS             16841..17428
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /locus_tag="E9649_RS02585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00513"
                     /product="YpmS family protein"
                     /protein_id="WP_002989129.1"
                     /transl_table=11
                     /translation="MKKKSNLNWWKWSFLCLLAFNTAFLMVIASRLIQVREPESELIAK
                     KPVKNIKIGTFVTTREQLNETVASYLKDYQTKKMSYKFYATSSSILFEGTYQLLGYEVP
                     LYIYFQPHRLENGAVQLQVISFSVGTLPLPEKDVLQYLKSSYKLPSFVKVMPNQSAIVV
                     NLQDIQNDAKVYLKAKKIDLFNDEISFNIYKK"
     gene            17527..17802
                     /locus_tag="E9649_RS02590"
                     /old_locus_tag="SAMEA3919117_00514"
     CDS             17527..17802
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1132: DNA-binding
                     protein HU 1"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /locus_tag="E9649_RS02590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00514"
                     /product="HU family DNA-binding protein"
                     /protein_id="WP_002983920.1"
                     /transl_table=11
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSTIEAFLAEGEKVQL
                     IGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     misc_feature    18046..18064
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.91"
                     /tool="antismash"
     misc_feature    18082..18095
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 26.23"
                     /tool="antismash"
     misc_feature    complement(18140..18154)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.54"
                     /tool="antismash"
     gene            18145..20007
                     /locus_tag="E9649_RS02595"
                     /old_locus_tag="SAMEA3919117_00515"
     CDS             18145..20007
                     /EC_number="7.2.2.-"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0015662 - P-type ion transporter activity
                     [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1037: heavy metal
                     translocating P-type ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /locus_tag="E9649_RS02595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3919117_00515"
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="WP_014407683.1"
                     /transl_table=11
                     /translation="MTIRQWMADHLHLMETLACLVLIIIGLAFLHSFPQVASAIFITAF
                     LIGGYASAKTGILDLVKNKHLSVDILMILAAIGAGIIGYWLEGALLIFIFSLSNTLEEM
                     AMEKSKDAISALMSLTPDTARQYQEDGHILEVETRSLSVGDRLQVRKGEAVPIDGQLLS
                     PFGQFDESMVTGEPITVDKAEGQDLIGGTINQGQTIDMLVTIENDDTLFAKIINLVESA
                     QEKKSKTATFIESLEDGYVKFVLVLIPAFILFSHFVLSWTWLAAFYRGMILLTVASPCA
                     LIASSTPASLAAISRAARKGLIIKGGDIVDNMGDIKAVVMDKTGTLTQGKPSVVNAHYL
                     EDELLVNRLVKGAEAASTHPISKALLEYTEKLEPLTFDHLEEISGKGFQGFYQGQEWRI
                     GKKTFILEKVQDLSAFEETIQVEENQGKTLIFVSRDHQLIAYYTLLDDIKIESKRAIKS
                     LHAMGIKTVMLTGDQERTANYVAQKLGIDEVVANCMPQDKVAKLAELKTKYGFVAMVGD
                     GINDAPALAQADVSYAIGSGTDIAMESADSVIMDDLTRIPFSIQLSRKMKTIIKQNIVF
                     ALSVITLLILANVFQVVNLPLGVVGHEGSTILVILNGLRLLSFK"
     misc_feature    20218..20230
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 22.13"
                     /tool="antismash"
     misc_feature    20283..20298
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.81"
                     /tool="antismash"
     misc_feature    complement(20315..20329)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.55"
                     /tool="antismash"
ORIGIN
        1 agaacttgta agaacatgtg catgacgtca ggatgggctt tttccacctc atctaagaga
       61 ataagtgaat atggattgcg acgaacttgt tcggttagct gtccagcttc ttcatagccg
      121 acataacctg gaggagcccc gactaatttg gcgacagcgt gtttttccat gtattcggac
      181 atgtcaaagc gaatcatatt gtttgtcgaa ccaaagagtt caatggctag ttgtttagac
      241 aattcagttt taccaacccc agtcggtcca acgaataaga aagaaccaat agggcgattt
      301 ggagttccta atcccacacg gttacgacga atagccttag cgattttatc aacagcatcg
      361 tcttgcccga tcacgtgtgc tttcagatca ttggctaagt ttacgagttg agactgttcc
      421 ttttctttta agtccccaac tggaatatta gttttttgct ctacaatagc ttcaatggtt
      481 ttttcagtaa tgatgggaat atcttgctca tcgacttttt gagcctgcat ttctttgtat
      541 tttgtaattt gatcgcggaa ataagctgcg cgttcatagt cttcgtctcg agtagcttgc
      601 gccttgagat tttcagcttc aataagacgt ttgtcaattt ctttaggatc aacaaagttg
      661 agggttagat tcattttgga accagcttcg tccagaaggt caatagcctt atcaggaaga
      721 aaacggtctt gaatatagcg gttagataaa tgagcggcag cttcaatagc ggctgggcta
      781 tatttgacat gatgatagtc ttcgtatttc ggttggatac cttttaaaat cgtgatggtt
      841 tcctcaacag aaggttcatc aactttaacg ggttgcatac gtcgttctaa ggcagcgtct
      901 ttttcaataa tacggtattc atttaaggtg gtagcgccga ccaactgcaa ttcaccgcgg
      961 gccaaggctg gttttaaaat attaccagca tccatattgc cgtctcctgc agaaccagca
     1021 ccgacaatct catgaatttc atcaataaag agaatcacat ccttgcgatt gcgaatttct
     1081 tccattaatt tttgcatgcg ctcttcaaac tgaccacgga tacctgttcc ctgaacgagg
     1141 ctaaccacat caagacgaat cacttgcttg ccttggagtt tttgaggaac agtaccatca
     1201 ataatttttt gagccaaacc ttctacaaca gcagttttac caactccagg ctcaccaatt
     1261 agcacaggat tatttttagt acggcggttg aggatctcga taacgcgtgt aatctcttcg
     1321 tcacgaccaa taacaggatc aatattacca tttcttgcaa tatctgtgac attaatccca
     1381 aactcttcta acaagccctt tgcttgctga tttggtgttt gtggctgagc aggtcgttgt
     1441 ccgttgtagt taccaccata gcgtcctccg ccatttccat tttgccctgc ctgagtaggt
     1501 ggggtatttg gaagattacc aaaagctcta aaattattca aatcaccaaa aaagtcatca
     1561 aagaaaggac ttgtggatct atcttgtgct ctatatcctg gggttaggcc atttaaaata
     1621 gaattggcag gatcggattt catgatttga taacaatttt gacagagatc aacctgtctt
     1681 tgttttccat taacacttgt ataaagatga atagtagatt cgtttaaatt acaattttga
     1741 cacagcataa tatacctccg agggtttatc taaaagtcaa agtcatttag tcaaaaatgg
     1801 tcaaacttta tattcatatt atagcaccac ggcttgggaa atcaagtaaa aagtttatcg
     1861 ctttagcgtt agcttttaga gagtaacatg acagagtaac tttattaagt ttccaatgag
     1921 caaatgttta aaatcattga aaagagtaaa aaaagtaaaa tatcaataaa tatattactt
     1981 tccttgttag tttcccttgt tttgtgttaa aataagaggg ataaaaaacg aataggagaa
     2041 acgaaatgga atcacatttg gtgagaatca tcaatcgttt ggagttgatg gtgacagatg
     2101 gcggtaatct caagcgaaat tttgaacgcg atggtgttgt tgttgcagaa gttgcctttt
     2161 caaatgatcc tgaaaatgga cctgttttca cattgcgtga tgttgaagcg cgtgagtcat
     2221 attcctttga tagtattgac ttgattgcaa tggaaatcta cgatttatta tattaagctt
     2281 gacatagtaa agagacttgc ctgaggcagg tttttttatt gtcaaaaagt attgtattaa
     2341 tattcatcag atggtataat tatctttatt gtaatgaacc taggacttat gaggagatat
     2401 aaatggattt gtcatttttg cccaaatact gggcctactt taactacggt gtacttgtca
     2461 ccattatgat ttcagtcagc gttgtctttt ttggaaccct tattggtgtc ttggtaaccc
     2521 tgattaagcg tagtcatgtg aagccgttga cctgggtcgt taatctttac gtttggatct
     2581 ttcggggaac acctatggtg gttcaaatca tgattgcctt tgcttggatg cattttaaca
     2641 atatgcctac tattggtttt ggggttttag acttggactt ttcaagacta cttcctggaa
     2701 ttattatcat ttcattgaat agcggtgctt atatttcaga aattgttaga gcaggtattg
     2761 aggctgtacc aaaagggcaa ttagaagcag cttattcact aggtattcgt cctcaaaatg
     2821 ccatgcgtta tgtgattttg cctcaggcct ttaaaaatat tttgccagcc ttaggaaatg
     2881 aatttattac cattattaag gatagtgctc ttttacaaac cattggagtg atggaacttt
     2941 ggaatggtgc ccaatcggtg gtaacggcta cttattctcc aatttcccct ttactggtgg
     3001 ctgcttttta ctacttaatg gtcacaacag tgatggcaca gttattggca gtcttagaat
     3061 gtcacatggc gcaaggaggt aatcattgat gactgaggct attatttcaa ttaaagactt
     3121 gcacaagtat tatgggcaca atgaagtgct taagggaatt gatttagaca ttatgccagg
     3181 agaggttgtg gtgattattg gcccttctgg ttctggaaaa tcgaccttac ttcgaactat
     3241 gaatcttttg gaagtaccaa ctaagggaca gattaggttt gaggggattg atattaccga
     3301 taagaagaat gatattttca gcatgcgtga aaaaatggga atggttttcc agcagtttaa
     3361 cctctttccc aatatgacta ttttagaaaa tatcacttta tcgccaatca aaactaaggg
     3421 aatggctaaa gcagaggctg acaaaacagc cttgagcttg ttggacaaag ttggattatc
     3481 agaaaaagcc aaggcttatc ctgctagtct ttctggtggg caacagcagc ggattgcgat
     3541 tgcgcgtgga ctggctatgg atccagatgt tttactcttt gatgaaccaa cttcagctct
     3601 agacccagaa atggtgggtg aggtcttggc tgtcatgcaa gatttggcta aatctgggat
     3661 gactatggtt attgtgactc atgaaatggg ctttgctaaa gaagtcgcag accgtgtgat
     3721 gtttatggat ggcggcgtta ttgtggaaga aggaagtcct aaccaacttt ttgatctgac
     3781 taaagaagaa cgtacaaaag attttttgag tagagtatta taaatctgtt ctatgaatta
     3841 taaaaggaag tcctaaatga tttaggactt ccttttataa tttgctaagg cattttatag
     3901 gagattactt ttctttttgt taaaataaaa agaaatagtt gcattatttg catagaagaa
     3961 agggatctgt tatggtttca gtgatgtggt ttcgtagaga tttaaggatt gatgatcaaa
     4021 aagctttagc tcatgctatt gctagtcaat caccaacctt atgtgtgttt caatttaaca
     4081 aggagcaatt aaatctgcta catagtcgga atcaatctag ttttattact agcgttttgg
     4141 cttttagaaa gttattaaaa tgtgaaggta ttgacttata tctaatgtat ggagacttaa
     4201 tgacctgttt tgagcaatta ttgacacaat taaaagactg gacagatgtg tattttaact
     4261 atgatgaatc tggatatggt cgtttacgtg atcaaaaagc agcacaattt ttcaaaaaaa
     4321 atgggattgc tgttcatacc taccaagatc actatttaca tggtagtcaa gagatcatta
     4381 accaatcagg tcagccttat aaagttttta cgccctacta taggatttgg caaaattatc
     4441 cgaaagagac tcctattaaa gtagaactat cgcaaggcag gtggttaaat cttgagacac
     4501 cagatgatgt tttaaggact gttgagagtt ttaaagatga aaaatatcag gatgttgcaa
     4561 cttttgatga agcgtcaaaa caactaaatc gatttattca agaccaatta gcagcttatc
     4621 acgctaatcg agattttcca gcgcagctag ggacgagtcg gttgtctcct tttttaagaa
     4681 taggagctat tggcattcgc acggtatatc acgctgtgcg tcaagcacca aatagtctag
     4741 ggcaagctac ttttttaaaa gaattagctt ggagagactt ttacaatatg gtctatgtgg
     4801 cttatcctga tcagaaaact cagcctatcc aaaaagcctt ttctcaaatc gaatgggtta
     4861 ataatcctga ctggtttcag ttgtggaaag aaggtaaaac gggttaccct attgttgatg
     4921 ctgcaatgct tcagttacaa aagacaggat ggatgcataa tcgtttacga atgatagtag
     4981 cttctttttt aactaaagat cttttatgtg attggcgttt aggagagcag tattttcaac
     5041 aacaactaat tgattatgat gctgccagta atatcggagg atggcagtgg gctgcttcca
     5101 ctggaacaga tgctgttcct tattttagaa tatttaatcc tgttactcaa ggtaagcgct
     5161 ttgatcccaa aggcgagttt attaaagcgt atttgcctca actagaacac gttccagaga
     5221 agtatctcca cgaaccgtgg aagatgccaa aaaaccttca agagagtgtg tcctgtatta
     5281 ttggcacaga ctatcctcaa ccaattgtcg atcatgctaa acaacgtgaa caagctattg
     5341 caaagtatga gtgggcaaaa gaaaaagcaa aaatagaatg actgttctca ttataagagt
     5401 ttaaacaaaa agttatcatg aattaagatt atttaattta aaaaataagt tgactttatt
     5461 tagattattt gatactattc agttgtctcc cttagagaaa gcgatttctt gttttggagc
     5521 tgatttttac catttggtct tagaatagga tgagtaatat gacttacaac gaggtatatc
     5581 aagaatggtt gcacaataat gatcttagtg atgatattaa agcagattta gcagccataa
     5641 aagacaatga ggctgagatt caagatcgtt tttacaaaac acttgaattt ggaacagcag
     5701 ggctaagagg aaaacttgga gcaggcacca atcgcatgaa tacctatatg gttggtaagg
     5761 cagcacaagc tttagctaat actattattg atcatggacc tgaagcagtt aaaaagggca
     5821 ttgcggttag ttatgatgtt cgctatcaat ctagaacatt tgcagaatta acatgctcta
     5881 ttatggcagc taacggtatt aaggcgtacc tttataaagg gattcggcca acaccaatgt
     5941 gttcgtacgc tattcgtgct ttgggatgta tctctggtgt tatgattacg gctagtcaca
     6001 atccccaagc ttacaatggt tacaaagctt attggcaaga aggttctcaa attttggatg
     6061 acattgcgga tcaaattgca caacacatgg ctgcactaac tcagtatcag gaaatcaaac
     6121 aaatgccttt tgaaaaggct ctggacagtg gacttgttac ttatattgat gagagtattg
     6181 aagaagcata taaaaaagaa gtgcttggtt taacgattaa tgatactgac attgataagt
     6241 cagtccgagt agtctatact cctttaaatg gtgttgggaa tctaccagtg cgtgaggttt
     6301 taagacgccg cggttttgaa aatgtttatg tggtacctga gcaggaaatg cccgatcctg
     6361 attttacgac agttggctat cctaatcccg aagtccctaa aacttttgca tattcagaaa
     6421 aattaggtaa ggcagttgat gctgatattt tgattgcaac tgatccagat tgtgaccgtg
     6481 tggctttaga agttaaaaac gcagtcggtg actatgtttt tctcaatggc aataaaatcg
     6541 gagcgctttt atcttactat atcttttcac aacgatttga cttaggcaat ttaccggcta
     6601 atcctgtttt agtgaaatcc attgtaacag gagacttgtc acgggctatt gctagccatt
     6661 atggtattga aaccgttgaa acattgactg gttttaagaa tatttgtggc aaggcaaacg
     6721 aatatgatgt gacgaaacag aaaaactatc tgtttggtta tgaagaaagt attggttttt
     6781 gctatggcac ttttgttcgt gacaaagacg ctgttagcgc ctctatgatg attgtagaaa
     6841 tggcagctta ctacaagaaa aaggggcaga atttactaga tgttttgcag actatttatg
     6901 cgacatttgg gtattataat gaacgtcaaa ttgccttgga gcttgagggg atagaaggac
     6961 aaaaacgcat tgcgaggata atggaagatt tcagacagac accaatagct agtgttgcag
     7021 agatggcgtt agacaagaca attgatttta ttgatggcta tcaagatttt ccaaagcaaa
     7081 actgtctcaa attttatttg gatgatggtt cttggtatgc acttagacca tcaggaacag
     7141 agcctaaaat aaaattttat ttatatacta ttggccaaac acaagaaaat agtgcaacaa
     7201 aattagatgc tattgaagca gcgtgtcgca caaaaataaa tcaggttaat taaacatcaa
     7261 aaatggataa cacttttaca ctatttttcc atataaacaa ctacaaaagg aggtgagact
     7321 tttaggcacc ttctttttct tgtaggtgcc actaatcaat ttaatcgact caaaccaata
     7381 ctaaaaaata agtaagagta taaagaacag gtatctattt ttttgttata atagaaaaat
     7441 gaaattaaag ggagatgtca atgacagaac taattgatgg taaagcctta gctcaaaaga
     7501 tgcaacaaga gttagcagct aaagtcaaca atctaaaaca aaaaaaagga attgtaccag
     7561 gcttagccgt tattcttgta ggtgatgatc ctgctagtca ggtgtatgtc cgtaataaag
     7621 agcgtgcagc tcttactgta ggttttaaaa gcgagaccgt tagattatca gaattcattt
     7681 gtcaagaaga gcttattgca gtaatcgaac gttacaatgc agataacact attcatggta
     7741 ttttagtgca gttacccctg ccaaatcata ttaatgataa aaaaattatt ctcgccattg
     7801 atcccaaaaa agatgtggat ggttttcacc cgatgaatac aggtcacctt tggtcaggac
     7861 gtcccttgat ggttccttgt actccatcag ggattatgga attgcttcga gaatataatg
     7921 ttaaccttga aggtaaacat gccgtcatta ttggcagatc gaatatcgtt ggaaaaccaa
     7981 tggcacagct cttactggac aaaaatgcaa cagtcacgtt gacacattca agaacacgtc
     8041 aattagaaga agtatgtcgc tgtgcagatg tgttgattgt ggcaatcgga caaggtcatt
     8101 tcataacaaa acaatatata aaagatggtg cgatagtgat tgatgtggga atgaaccgtg
     8161 atgataatgg caagctaatt ggagatgtgg cctttgatga ggtggcagaa gttgcagcga
     8221 aaatcacccc tgtaccagga ggtgtcggtc ctatgacgat tgctatgttg ctagagcaaa
     8281 cttatcaatc tgccctccgt agtactcata aatagttata attagacaat tcaacataag
     8341 agaatataag aagtcagcaa tggcttcctt ttttgttttt ctgctcacta tttatccaga
     8401 aaaatggtat aattgcatta gtatgcaaag ggactatgtc aggtccaact aagtgaaagg
     8461 tggcttgatg gcagattatt taaccgtcac tcatttgacg aaatatttaa aattaaaatt
     8521 tgaccgtgac ccttatttgg aacgggttta tctgactggt caagtgtcca attttcgaaa
     8581 acgaccaact catcaatatt tttccttaaa ggatgaaagt gctgtgattc aagccaccat
     8641 gtgggtagga gtctataaaa aactaggatt cgacctagaa gaaggcatga aaattaatgt
     8701 aattgggcga gtccaacttt atgaacctag tggctcttac tctatcgtga ttgaaaaggc
     8761 agagccagat ggtataggtg ctttggcttt gcagtttgaa caattaaaga aaaaattaac
     8821 ggcagaaggt tattttgagc aaaaacacaa gcagcccttg ccacagtttg tatcaaaaat
     8881 tggggtcatt acaagtccta gtggtgctgt gattcgagat attatcacaa ccgtttcacg
     8941 gcgttttcca ggggttgaga ttttattatt cccaactaaa gtacaaggtg atggagccgc
     9001 ccaagaagta gtggctaata ttcgaagagc caatcaaaga gaggatttgg acttgctcat
     9061 tgtcggccgt ggaggtggct cgatagaaga cctttgggcc ttcaacgagg aaatagtggt
     9121 tcaggctatc tttgaatcgc aacttccagt gatttcgagt gtgggtcatg aaactgatac
     9181 aaccctggca gattttgtgg cagatcgcag agcagccaca ccaacagcag cagcagagtt
     9241 agcaacgcct attacaaaaa cagatcttat gtcttggata gtagagcggc aaaatcgttc
     9301 ttatcaggcc tgcttgaggc ggattaagca acggcaagag tgggtggata aactatcaca
     9361 atctgttatt tttaggcaac cagaacgatt atatgatgcc tacttgcaaa aaattgatcg
     9421 ccttagcatg accctgatga atacgatgaa agatcgtctg agctccgcca aagaaaacaa
     9481 ggttcagttg gaccatgcct tggccaatag tcaattacag acaaaaatcg agcgatacca
     9541 agaccgtgtt gctaccgcta agcgtttact gatggctaat atgacaaatc agtacgatag
     9601 ccagctggcc cgatttgaaa aagcccaaga tgctttgcta tcactggatg ccagccgaat
     9661 tatcgcccgt ggttatgcaa tgattgaaaa aaatcaagca ctggtagcct ctgtaagtca
     9721 gataacaaag ggtgatcagt taaccattaa gatgcgtgat ggacaattag atgtagaggt
     9781 aaaagatgtc aaaaacgaaa acatttgaag aaaatttaca agatttagag actattgtga
     9841 acaaacttga aaatggggat gtgcccttag aagaagccat ttcagaattt caaaaaggaa
     9901 tgcttctctc aaaagaactt caaaagacct tacaagcggc agaaaaaaca ctcgtcaaag
     9961 tgatgcaagc tgatggcaca gaagtagata tggatgatta atggacaaat tagctagaat
    10021 tgacgaagcc attcgtcgct actataagac gactagtaac ggtgtatctg aagagttgat
    10081 tgatgctatc ttgtattctg ttgacagtgg tggcaagcgc attcgcccac ttatcttatt
    10141 agagatgatt gagggatttg gggtatcgtt acagaatgct cattttgatt tggctgcagc
    10201 ccttgaaatg attcatacag gaagtctgat tcacgatgat ttgccagcca tggataatga
    10261 tgattaccga cgtggtagac tgaccaatca caaacaattt ggagaggcta ctgctatttt
    10321 ggcgggagat agcttatttt tagacccctt tgggttaata gctcaagctg aattaaatag
    10381 cgaggttaaa gtagccttaa tccaagaact ttccctagct tcaggaactt ttggcatggt
    10441 tggtggtcag atgctagata tgaaagggga aaatcaagct ttgagtctcc ctcagttgtt
    10501 actgattcac ctcaataaaa ccggaaaatt attaactttt ccttttaaag cagcagctct
    10561 tgtaacagaa caggctatta ctgttcgtca acaactagag caggcgggaa tgctcatcgg
    10621 gcacgccttt cagattaggg atgatatttt agacgtgaca gctagctttg aagatcttgg
    10681 aaaaacgcct aaaaaggatt tatttgcaga aaaagctact tacccaagtt tattggggtt
    10741 agaagcctcc taccaactgt tgacagagag tttagatcag gctttgacga tttttcagac
    10801 actcgaaagc gatgtaggct ttaagcctca aataattaca aaactgatag aagggttacg
    10861 acttaatgcc taaagaaaga gtagatgtgt tagcctataa gcaaggatta tttgagacca
    10921 gagagcaagc taagcgtggt gtcatggcag gcttagtggt ctctgtgatt aatggccaac
    10981 gctatgacaa gccaggtgac aaaattgacg atgggactga gttaaaactt aaaggtgaaa
    11041 aactcaaata cgtcagtcgt ggtggactaa aactggaaaa ggggctgcac gtttttggtg
    11101 tatcagttgc taatcaaatt gggattgata ttggcgcttc aacaggtggt tttaccgatg
    11161 ttatgttaca agatggggcc aaactagtct atgccgtcga cgttgggact aaccagttgg
    11221 tatggaaact cagacaagat ccacgagtaa gaagtatgga acagtataac tttcgttatg
    11281 ctcagccaga agactttaat gagggacagc ctgtatttgc cagtattgat gtgtccttta
    11341 tttctctcag cttgattttg ccagccctgc ataacgtttt gtcagatcag ggacaggtta
    11401 ttgctctcat taaaccgcaa tttgaagctg ggcgcgagca gattggtaaa aaaggcatcg
    11461 tcaaggacaa acagattcat gaaaaagtga ttcaaaaggt catggatttt gcctcaggtt
    11521 acggatttac agttaaggga cttgattttt cccctattca gggtggtcat ggcaatatcg
    11581 aatttttagc tcatcttgct aagtcacaga cacctgaaac gttagccccg catttgattc
    11641 agaaagttgt tgccaaggca cataaggagt ttgagaaaca tgaaaaagag tgaacgactt
    11701 gaattaatca aaaaaatggt tttgacgcat ccaattgaga cgcaacatga cctcttaaga
    11761 ttattggcag agcatgggtt agaattaacc caggctacta tttcaagaga catgaatgaa
    11821 attggtatag taaagattcc ttctggcagt ggacgttaca tctatggtct ttctcaagat
    11881 agtggaaaaa aaatcgttca aggacctaga tcgataaaga gcactatttt agctgtatct
    11941 gacaaaacaa aaggtttaga acaacacctc tatttaaaag tcgtacctgg caatagtaaa
    12001 ttaatcaaac gttatttatt agcagatttt tcaaaagcta tttttagtct tattgctgat
    12061 gatgatagtt tattgttaat tgcaaagtct ccttcagaag cagatatgat acgccaagaa
    12121 attttgcttt ggatgcaggg tataacctag aaaaaaatag gggagaaagt gatgttatta
    12181 gaaatttcta ttaaaaattt tgctattatt gatgaaattt ccctaaattt tgaaaatggt
    12241 atgacggttt tgacgggtga aactggagca ggaaagtcta ttatcattga tgctatgaat
    12301 atgatgcttg gtgcgcgtgc tagtacagaa gtgattcgtc gtggagctaa taaagcagaa
    12361 attgaggggt tcttttcggt agatgctacc ccagaactgg ttgcgtgtct ggaatcatca
    12421 ggtattgcta tggaagaaga actgattatt cgccgagata tctttgctaa tggcagaagc
    12481 gtgagccgca ttaatggtca gatggttaac ctagccacct taaaacaggt cggacagttt
    12541 ttggtagata ttcatgggca acatgaccaa gaagaattaa tgcgaccaca gctccaccag
    12601 caaattttgg acgcttttgg tgataaggct tttgagcaat tgaaagagaa ctatcagctt
    12661 atttttgatc gttataaaag cttgcgtcgc caggttattg acaaacaaaa aaatgaaaag
    12721 gaacacaaag atcgtattga tatgttggcc tttcagatag cagaaattga agctgctgcc
    12781 ttgagtcgag gtgaggatga ccggttaaat caagaacgtg atcgcttaat gaaccacaaa
    12841 caaattgctg ataccctgac caatgcctac gtcatgctag ataatgacga tttttcaagt
    12901 ctatccaata ttcgctctag catgaatgac ttactatcaa ttgagcagtt tgattcagag
    12961 tacaaaggga tgtcgacttc gatttctgaa gcctattata ttcttgaaga agtgagcaag
    13021 caattatcag ataccattga ccaactggat tttgatggtg ggcgattaca ggaaattgaa
    13081 tttcgcttgg atacactcaa tagcttaacc cgtaaatacg gtggtaatgt gaatgatgtg
    13141 cttgactact acgataatat tatcaaagaa taccagctat taacaggaga cgacttgtct
    13201 tcaggggatc tagaatcaga attgaaagcc ttagaaaaac aattggttgc tgcagccact
    13261 aacttgagtg aacgccgcca tcaattggca gagcaattag aagctgagat taaagctgaa
    13321 cttaaggaac tttatatgga aaaagcagat ttcaaagttc actttaccac atcaaaattc
    13381 aatcgtgatg gtaatgaaag cttggaattt tacatctcta ctaatccagg cgaaggattt
    13441 aagccccttg tcaaggtggc ttctggagga gagttatcac gccttatgtt agccatcaag
    13501 gctgctattt ctagaaaaga agataagacg agtattgttt ttgatgaagt agatacaggt
    13561 gtttctggtc gcgttgcgca agccattgcc caaaaaattt ataaaattgg gcgacatggg
    13621 caagttctcg ctatttcaca cttacctcaa gttattgcta ttgctgatta tcagtatttt
    13681 atttctaaag aaagtaaaga agagtccacc gtttctaagg taaggttatt aactcctgaa
    13741 gaacgtgtgg aagaaattgc tagcatgatt gcaggaacag atatgacaca agctgctctt
    13801 acgcaggctc gtgaactact tgccaaacat taagattatg tttggtattt gatattttca
    13861 taattagatg aaattgattt tttaggtact aaaataatca ttgtgctaga ccatttttcc
    13921 tctgttaatc tggcttggtt tgccttgaca aacctctgag aaatagtaga atagataaga
    13981 gaattttaag ctgaggggat agaaatgact agtaaaaaag cgtgtttatc aagcatcatt
    14041 gtgttagcaa gtttaacgtg tggaaatgat actgttagtg ccaatcatct ctcagcaact
    14101 ggagataagt ttgatgattg ctcaacactt gttgaaaaag atgtggcccc taaagatgaa
    14161 cttgagatgt tagcatggtc ctcgtctcaa acaactgatg atgctgacag agactatgaa
    14221 gattttctcg atgatgattc ttttaatgat gattctttta tttctcaaaa tgaaactgat
    14281 aagatgtttg agaatttaac tgatgatagg ttattaaatg aattagatga atcagatgaa
    14341 tcagatgaat cagatgaaga agttacaatt gagccagagc aaaatgtaat aatgcctagt
    14401 gacgatgagc tatttgattt aactgatgct gttgagacac gccttactgt ttctagtgct
    14461 ccccatttag aggctgaatt gccgaaacca catttgagga gcctatcaga tacagcactg
    14521 cggtctggtg aaattagagg acatttagat aacaaactgg acgctttgtc tgtaacagct
    14581 acaaagttag cattaacgat ggctcaaaaa tttgatttga caacgcatgt ctattctata
    14641 ggtgaaagct ttagtgaagt attagctgct cattatgaag atagaaaagc agaatcagct
    14701 ttttctaaga aaaagagatt tcaccttcct attgctactc cagatgttgt tatagaggag
    14761 ttaaggcgcc tagtctcttc tattggaagt tcaaaagaag atgtttcagt tccttatagt
    14821 cggaagctag gtatggcagt tgcaaaaaga aaaatagccc tgccacaaac gggagagggg
    14881 ttctcttatt atccagcttt acttggttta atgatattag gattaacgcc gattatgata
    14941 ccaaagaaga taaataatta ggtaataagt tattgtagtt gtaaaagacg gggcaaatgt
    15001 tcaagcgtga aaaggttgac ttttgcctcg ttttgtatct cgttattgct aaggcatttt
    15061 cttcgagcag ctattgctcg ccatacctat ctaccgagtg attattcaat agcactacca
    15121 gttttgcttt atttagtaag gagtgttaga ataaagaggt gagaaactag tttagaatgg
    15181 aaaaatacat gggaactata aaaattgtta cagattcatc aataactatt gaaccagaat
    15241 taattaaagc tttagatatt actgtagtgc ctttatctgt catgattgat agcaaattat
    15301 attctgataa tgatttaaaa gaagaaggtc attttttgag tcttatgaag gctagcaaat
    15361 cccttcccaa aaccagtcag ccaccggttg gtctctttgc tgaaacttac gaaaatttgg
    15421 tcaaaaaagg agtaactgac attgttgcta ttcacctctc accagcttta tcaggtacta
    15481 ttgaagcttc ccgtcagggg gctgaaatcg ctgaggcacc tgttacagtt ttagattcgg
    15541 gatttactga tcaagccatg aaatttcagg tcgttgaggc agcaaagatg gcaaaagcgg
    15601 gtgctagttt gaatgagatt ttggctgttg ttcaagctat caaatcaaaa acagagctct
    15661 acattggtgt atctacctta gaaaatttag taaaaggagg tcgtattggt cgtgtgacgg
    15721 gtgttttgag ctcgctactt aatgtcaaag ttgttatggc acttaaaaat gatgagttaa
    15781 aaacgcttgt caaaggtcga ggtaataaaa catttacgaa atggttagat agttacctcg
    15841 ctaaaaatag tcatcgcccg attgcagaga tagctatttc ttatgctggt gaagctagcc
    15901 tggcattaac cttaaaagaa agaatcgcag cttattataa ccattctatt tcggttttag
    15961 aaacaggctc tatcatccaa acacatacag gagaaggagc ttttgcggtt atggttcgtt
    16021 atgaataatc gtcatttatt tagtgggata tttttctttg ttattagttt atgtctggcc
    16081 tttttattgc taaatattat tatccctaag tcaaattcac gtttgaaaaa gagtgatttt
    16141 ctgaaaaaag aacaagtagc tatccaatat gttgctatag gagattcatt gacagaagga
    16201 gtaggtgatc taactcatca aggtggtttt gttcctttgt taacgaatga tctcagtgaa
    16261 tattttaagg ctaatgttaa tcatcaaaat tacggtgtat ctggtgatac cagtcaacaa
    16321 attcttgata ggatgataaa acaaaagcag atacagttat ctttaaaaaa agcagatata
    16381 atgacgttaa ccgttggtgg taatgatgtt atggcagtta ttcggaagaa tttagcggat
    16441 ttgcaagttt ctagttttag aaagccagct cgtcagtatc aaaaacgatt aagacagatt
    16501 atcgagttag ccagaaaaga taataaagat cttcctattt ttattttagg catctataat
    16561 ccgttttatt tgaattttcc agaactaact gatatgcaaa aagtgattga tgactggaat
    16621 accaaaacta aggaggttgt tggagaatac gatcgtgtgt actttgtgcc aataaatgac
    16681 ctcttgtata aagggataaa tggacaagaa ggaattgttc attcttcagg agatcaaact
    16741 acaattgtca atgatgcctt gtttactggg gaccattttc acccaaataa tactggctat
    16801 caaatcatgt caaatgcagt aatggagaaa ataaaaaagc atgaaaaaaa aatcaaacct
    16861 taattggtgg aagtggagct tcttatgtct gcttgctttt aataccgcct ttttgatggt
    16921 tattgcaagt cgtctcattc aagtaagaga acctgagtca gaactgattg ctaaaaaacc
    16981 agttaaaaat atcaaaattg gtacatttgt cacaaccaga gaacagctta acgaaactgt
    17041 agcaagttat cttaaagatt atcagactaa aaaaatgtct tataaatttt atgcgacatc
    17101 ttcttctatt ttgtttgaag gaacttatca attattaggg tatgaagtcc ctttatatat
    17161 ctattttcaa cctcatcgtc tagaaaatgg agctgtccaa ctacaggtga tctctttttc
    17221 agtgggaact ctgccactcc cagaaaaaga tgttttgcag tatttgaaat caagctacaa
    17281 attgccaagc tttgttaaag tgatgccaaa tcaatcagct atcgttgtta atttacaaga
    17341 tatccaaaat gatgctaagg tttatttaaa ggccaagaaa attgatttat ttaatgatga
    17401 aatcagtttt aacatctata agaaataagc tcaaataacg ttaaatactt gacctaatcg
    17461 ctttttttag atatgataat atctgttaaa gcattaagct tactaattat ttggaggatt
    17521 tgttaaatgg ctaacaaaca agatttaatc gcaaaggttg cagaagcaac tgaattgact
    17581 aaaaaagatt cagcagcagc agttgatgcc gtgttttcta caatcgaagc tttccttgct
    17641 gaaggtgaaa aagtacaatt gatcggtttt ggtaacttcg aagtacgcga acgtgcagct
    17701 cgtaaaggtc gtaacccaca aactggtgca gaaattgaaa ttgcagcttc aaaagttcca
    17761 gccttcaaag ctggtaaagc tcttaaagac gctgttaaat aattcgtcta gaaaaatctt
    17821 gttgctatcg atgtttattg atagcgacaa ggttcttttt tatatttggg gcataaagtt
    17881 agtagttttc tcttcagtta agccaagttc taatgcatgc actctataat ggtcagtcat
    17941 ccttctatta gtatatcatt tccgcaggta gggggagttt ttttcatatt tgcaaatagt
    18001 cgaagttagg atttttgaaa gcaaaaggta agtttaaaaa agtttattta gaatgcttct
    18061 aaattacttg acttatttat ttagaattat tataattaat gtatcttaaa gattgaataa
    18121 cttttaattg cgagggaact aaaaatgaca atcagacaat ggatggcaga ccatcttcat
    18181 ttaatggaga ctttagcgtg tttggtatta ataataattg gcctagcctt tttacactca
    18241 tttccacaag tggcttccgc tattttcatt acagccttct tgattggagg atatgcgtct
    18301 gcaaaaacgg gtatattgga tttggtgaag aacaaacact tgtcagtgga tattttgatg
    18361 attttagcag ctatcggtgc tggaattatc ggctattggc tggagggtgc tctgcttatt
    18421 tttatctttt cgttgtccaa tacgcttgaa gaaatggcca tggaaaaaag taaggatgct
    18481 atttcagcct tgatgtcctt gacgccagat acagctcgtc aataccaaga agatggccat
    18541 attttagaag ttgagaccag atccttgagt gttggtgacc gtttacaagt tcgtaaaggc
    18601 gaagctgttc caattgacgg gcagttgctt agtccttttg gtcaatttga tgaatctatg
    18661 gtcactggtg agcctatcac tgttgataag gcagaaggcc aggatctgat tgggggaacc
    18721 attaaccaag gacaaaccat agatatgttg gttactattg aaaatgacga tactctcttt
    18781 gccaagatta ttaatctggt ggaatctgcc caagaaaaga aaagcaaaac tgccaccttt
    18841 atcgaaagct tagaagatgg ttatgtcaaa tttgtgctcg tccttattcc tgcctttatc
    18901 ctctttagcc actttgtgct ttcttggact tggttggctg ctttttaccg agggatgatt
    18961 ctcttaacag tagcttcacc atgtgcccta attgccagtt ccacacctgc tagcttggct
    19021 gctatttctc gtgcagccag aaagggattg attatcaaag gaggggatat tgtcgataac
    19081 atgggagata ttaaggctgt tgtcatggat aaaacgggaa cgctcaccca aggaaaacct
    19141 tctgttgtga atgctcatta tttggaagat gagttgctcg tgaatagact ggtaaaagga
    19201 gcagaggctg ctagtaccca tcctatttct aaagcccttc ttgaatacac tgaaaaattg
    19261 gagccactga cctttgacca cttagaagaa atttctggga aaggttttca gggcttctat
    19321 caagggcaag aatggcgaat tggcaagaaa accttcattt tggaaaaggt tcaagaccta
    19381 tcagcttttg aagaaactat tcaagtggaa gaaaatcaag ggaaaaccct aatctttgtt
    19441 tcacgtgacc atcaattgat agcttactat accctcttgg atgatatcaa aatagaatca
    19501 aaacgtgcta ttaagtctct tcatgccatg ggaatcaaaa cagtcatgtt aacaggtgac
    19561 caagaacgaa ccgccaatta tgtggcacaa aaacttggta ttgatgaagt ggtagccaac
    19621 tgtatgcctc aagataaggt ggctaagtta gcagaattaa agactaaata tggttttgtg
    19681 gctatggtag gagatggtat taatgatgct cctgcccttg ctcaagcaga tgtttcttat
    19741 gctattggat caggaacaga tattgcaatg gaaagtgcag acagtgtgat tatggatgac
    19801 ttgactcgta ttccattttc gattcaactt tcccgcaaaa tgaagaccat tatcaaacaa
    19861 aatattgttt ttgccttatc tgtgattacc ttattgattt tagccaatgt ttttcaggta
    19921 gttaacttgc cgcttggtgt tgttggacac gaaggctcaa cgattttagt gattttaaat
    19981 ggcttgcgtt tactttcttt taaataattt agaaacttgc ttgaaaactt gaaagtgttt
    20041 ataataatag taagacttat gaaagagaaa accgccgtat gtttactatt atgatcattg
    20101 gtttagctgt attttcaata attaaaatca ttctattttt ctatttttat cagcaatttg
    20161 agcgcaagca aaaaagaaaa gttgaatcaa acaaccagca aagacagtcc caagaaagtg
    20221 acgctgtccc ttcaaaaaaa ttcccatttt aaaaagcttg gaaagtgtac aaagtgcaca
    20281 tttccaagct tttttgacta ttaaggttaa tctttgtaac gtaagttccc acgaaaatcg
    20341 tctaagcttt ggtagccttt ttctaccata atcgttttta attccttggt cacccgctca
    20401 aagatagccg gtccttcttg atgaagagcc gtaccaattt gaaccatact ggcgccacaa
    20461 agaatatgtt caaaggcatc gcgtcctgtt ttaactcccc ctgtaccaat aatgtggata
    20521 gaaggtttta agcgtttgta aaacgcatgg acattggcta gagcagtagg tttaatataa
    20581 tctccaccaa tgccaccaaa gccattttta ggcttgatga ggacttgctc atcctcaagt
    20641 actagaccat ttccgataga attgacacag ttaacaaagg aaaggggata tttattgaaa
    20701 atagcagccg cttgatcaaa atgcacaatg tcaaaataag gaggtaattt gataccaaga
    20761 ggtttagtgt aataggtaaa gatgttctct agaagttgat cagtggtttc aaaatcataa
    20821 gcaatctgtg gttttcctgg aacgttaggg caagaaaggt ttagttccac tag
//
