LOCUS       NZ_LR134267            21543 bp    DNA     linear   CON 15-APR-2024
DEFINITION  Staphylococcus pseudintermedius strain NCTC5661 chromosome 1.
ACCESSION   NZ_LR134267
VERSION     NZ_LR134267.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus pseudintermedius
  ORGANISM  Staphylococcus pseudintermedius
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus; Staphylococcus intermedius group.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2019) WELLCOME SANGER INSTITUTE, Pathogen
            Informatics, Wellcome Sanger Institute, CB10 1SA, United Kingdom
REFERENCE   2
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2018) WTSI, Pathogen Informatics, Wellcome Trust
            Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900636355.2-RS_2024_04_15
            Annotation Date                   :: 04/15/2024 03:28:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,432
            CDSs (total)                      :: 2,350
            Genes (coding)                    :: 2,313
            CDSs (with protein)               :: 2,313
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 12 of 37
            Pseudo Genes (incomplete)         :: 27 of 37
            Pseudo Genes (internal stop)      :: 6 of 37
            Pseudo Genes (multiple problems)  :: 8 of 37
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:45:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2418381
            Orig. end                         :: 2439924
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR134267.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21543
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="sactipeptide"
                     /region_number="8"
                     /rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /tool="antismash"
     cand_cluster    1..21543
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /kind="single"
                     /product="sactipeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21543
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11543](+)"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11543
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
     misc_feature    complement(361..376)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.51"
                     /tool="antismash"
     misc_feature    940..959
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: medium, score: 23.74"
                     /tool="antismash"
     misc_feature    943..957
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 22.46"
                     /tool="antismash"
     misc_feature    1003..1018
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 23.22"
                     /tool="antismash"
     misc_feature    complement(1253..1267)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 19.72"
                     /tool="antismash"
     misc_feature    complement(1881..1895)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 20.25"
                     /tool="antismash"
     misc_feature    2161..2174
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.06"
                     /tool="antismash"
     misc_feature    complement(2299..2312)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: weak, score: 15.72"
                     /tool="antismash"
     gene            2711..3589
                     /locus_tag="EL085_RS11395"
                     /old_locus_tag="SAMEA3504576_02342"
     CDS             2711..3589
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614835.1"
                     /locus_tag="EL085_RS11395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02342"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_014614835.1"
                     /transl_table=11
                     /translation="MELKQLKYFVEVAKREHISEAALELNVAQSAISRHIHNLENELET
                     SLFYRRGRNVFLTAEGKQLLEYAQQILEQVDLTLSQFQTQVQQQQSVFTIGYVDGSIGQ
                     ILPQVLQTIENELSLSLIPTLLDEQETLQALRAQEIDFALTTVTTTDPTFEIVPLVEET
                     YVLYGDLHAPMMNVPNPPLSHILKQPLYLLEPIPTDFKTYLITHADKPVRVLNQEQFAR
                     FILRNQKGFVLAPTYVNLYSQNQQWKKISLSHTDFKKTLRLIYRRDLQKPLRDEVIQII
                     MTHIQQRSVYH"
     gene            3602..4711
                     /locus_tag="EL085_RS11400"
                     /old_locus_tag="SAMEA3504576_02343"
     CDS             3602..4711
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614836.1"
                     /locus_tag="EL085_RS11400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02343"
                     /product="YibE/F family protein"
                     /protein_id="WP_099987168.1"
                     /transl_table=11
                     /translation="MKQLSKKINTIVIVVSIIVAICAIVFTRLNSSFYHIPLGEVTKIE
                     SHHQEKSVDEQHNQDVKYKDVLQVKLLNTKQQGDTLHVEHRYNASKTEEQAYQPGDKVL
                     LHKGKNQKDTYIIEKKRDTVLVTVISAFFIALLIVGQRIGLQSILSLVINAFAILLAIS
                     LYHAYPQLNLFLLMSLAVMIATILTLWLVIGWSMRTIVTIVSTLLGTFICIFIAWSVIG
                     LTHGQGIKYETMSFLTIQPRTVFLTSVMVGTLGAVMDVAITISSGMYEVLKRSPHISMQ
                     RWVLAGRNIGRDIMGTMTNILLFSYLAGSLPMLLLYLKNGNTLTYSISMNWSLEISRAI
                     TGGIGIALTIPLTIFLMRIWLSLRGGATR"
     gene            4708..5481
                     /locus_tag="EL085_RS11405"
                     /old_locus_tag="SAMEA3504576_02344"
     CDS             4708..5481
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220087.1"
                     /locus_tag="EL085_RS11405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02344"
                     /product="YibE/F family protein"
                     /protein_id="WP_014614837.1"
                     /transl_table=11
                     /translation="MNAIVILGLILFILMLIFGGKTGLVSFLTLFLNFIILFITVLAIV
                     FGAPIYVVTFIFCIIVSMVNLFLLNRFNTKTLAAFIASTVTTLLMIVAVYLSVHWGHLQ
                     GFTQEEQDETYIFSLNIGIDMEQFMIFTVLLAVIAAVIDLAITISSPVFELHETNPSLS
                     ERELFHSGMRVGREILATSANTIYLALIGGSMTIVFWFFNLHYSFGHMLNAKLFTQEVI
                     TIALGGVAIAVCIPITAMITAWLVKHRHQLPFHLD"
     gene            5530..7791
                     /locus_tag="EL085_RS11410"
                     /old_locus_tag="SAMEA3504576_02345"
     CDS             5530..7791
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166373.1"
                     /locus_tag="EL085_RS11410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02345"
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="WP_063279044.1"
                     /transl_table=11
                     /translation="MTAIHIHGARQNNLKNIDVSIPKHQLTVVTGRSGSGKSSLVFNTI
                     AAESERLLNETYSSYIQHQLTQYEKPDVDHIENLPVAMVINQKRLGGNSRSTVGTISDI
                     YASVRLLWSRIGTPFVGYSDVFSFNNPNGMCEHCQGLGYVEDIDLNELLDFDKSLNEGA
                     IRFPSFKPDSWRGKRYRYSGLFDNDKKLKDYTKEELDTFLYTEPTRLKNPPSEWPKTAK
                     FEGLIHRFRRSFLINDNFEKKRFLKDVERVVTKQTCPVCHGQRLNQKVLSCKIHGLNIA
                     DFTALTIEETLPFLEQIDSDKATYIIEPLKAQLQALNDIGLNYLTLARETTTLSGGESQ
                     RIKLIRHLNSPLTDLVYIIDEPSVGLHPADIEKINEIMLNIRDKGNTVIIVEHDPDVIK
                     IADHVIDIGPGAGKHGGQITFEGSYAQLKQSDTDTARALTRQHHLKQSAIRDNQAWLHL
                     EHLQHNNLQDVSVAIPKNAQTVVTGVAGSGKSSLIKGGFAKHEDAILIDQKGVHTSNRS
                     NLLTYTGIFDDIREFFSKATGLKKSMFSYNSDGACPNCNGKGVLKTELAFMPDFSQVCE
                     VCGGTRYRPEVLETKVDGYSIADVLALTVEEGLSLFQTHDAIAQTLSALQSTGLGYMTL
                     GQSLDTLSGGEIQRLKLTRYMIEPVTEKIFIFDEPTTGLHEDDIPILQARFDQLIEEGH
                     TVILIEHNITMMTRADWLIDVGPGAGNQGGRILYSGPPAGLKNVAESRTAPHLFGSLSE
                     "
     gene            complement(7858..8178)
                     /locus_tag="EL085_RS11415"
     CDS             complement(7858..8178)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /locus_tag="EL085_RS11415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_070407722.1"
                     /transl_table=11
                     /translation="MKQSTKSNTNKSLFKNLTWDYFKAFINKQLSDPKTKHIYQKRKID
                     VESTFVNLKANLGFQRLSVRTQSKVECELGIALMAVNIRKLAKISARFRSLIRKKPSNS
                     KN"
     gene            complement(8182..8781)
                     /locus_tag="EL085_RS11420"
                     /old_locus_tag="SAMEA3504576_02346"
     CDS             complement(8182..8781)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /locus_tag="EL085_RS11420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02346"
                     /product="transposase"
                     /protein_id="WP_126511029.1"
                     /transl_table=11
                     /translation="MRQSKKAIQDFKDRKLKYDKQMEIYGDRKSYSKTDYDATFMRMKD
                     DHMRNGELKPGYNLQIATNNQFVLAFGVYSYPGDTRTLKPFLKSIHKLYGDIPEYIVAD
                     AGYGSEYNYTVILDEFEKTPLITYSMYLKEKQRKYKNNPFITANWEYNVIEDYYICPNK
                     KTLHFQSYRKRRDGYGIQRDFKLYACEACVGCPLRS"
     gene            complement(9239..9877)
                     /locus_tag="EL085_RS11425"
                     /old_locus_tag="SAMEA3504576_02347"
     CDS             complement(9239..9877)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0030552 - cAMP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1133:
                     Transcription regulator, crp"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /locus_tag="EL085_RS11425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02347"
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_126511030.1"
                     /transl_table=11
                     /translation="MVELLGIKEIIKEKGINNKLCKGEYFQENFEKYIYLVDKGILYVH
                     QDNIDGTTLIYKIIGPDSLFICDNSKYFCGKTEVVFYKMLISSIINDEKICREYIKLLI
                     NENVKKDMFIRDMLTKDKVCRLLSILVRLANTFGEEEGRMIVIKIKITQEELAQYCGTT
                     RENIARIMKQLKDRKIVDTSSRFIKILSLEEIKQMIPCEGCNSFVCKTF"
     gene            10001..10132
                     /locus_tag="EL085_RS11430"
                     /old_locus_tag="SAMEA3504576_02348"
     CDS             10001..10132
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: Subtilosin_A"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020361779.1"
                     /locus_tag="EL085_RS11430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02348"
                     /product="subtilosin A family bacteriocin"
                     /protein_id="WP_037544023.1"
                     /sec_met_domain="Subtilosin_A (E-value: 6.5e-22, bitscore:
                     74.7, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKGIMVSNKGCSTCSIGAACLIDGPLPDFEVMGITGIFGLTS"
     CDS_motif       10001..10030
                     /prepeptide="leader"
                     /note="peptide class: sactipeptide"
                     /note="predicted sequence: MEKGIMVSNK"
                     /locus_tag="EL085_RS11430_sactipeptide"
                     /aSTool="sactipeptides"
                     /tool="antismash"
     CDS_motif       10031..10132
                     /prepeptide="core"
                     /locus_tag="EL085_RS11430_sactipeptide"
                     /peptide="sactipeptide"
                     /predicted_class
                     /score="31.00"
                     /molecular_weight="0.0"
                     /monoisotopic_mass="0.0"
                     /core_sequence="GCSTCSIGAACLIDGPLPDFEVMGITGIFGLTS"
                     /aSTool="sactipeptides"
                     /tool="antismash"
                     /leader_sequence="MEKGIMVSNK"
                     /leader_location="[10000:10030](+)"
     gene            10208..10840
                     /locus_tag="EL085_RS12420"
                     /old_locus_tag="SAMEA3504576_02349"
     CDS             10208..10840
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: subtilosin"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Subtilosin_rSAM_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /locus_tag="EL085_RS12420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02349"
                     /product="radical SAM protein"
                     /protein_id="WP_243134262.1"
                     /sec_met_domain="subtilosin (E-value: 2.9e-72, bitscore:
                     240.5, seeds: 6, tool: rule-based-clusters)"
                     /sec_met_domain="Subtilosin_rSAM_RRE (E-value: 2.3e-21,
                     bitscore: 73.1, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 5.8e-06, bitscore: 23.0,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 3.8e-11, bitscore: 40.8,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQYIFFNDNARLHQLPHGGVIEVKNGMFKDEVFDFEYLDLNSSA
                     FEACTYFDGSMTIIDILKDMCQKYKCDINDHIEWYTNLVNDLYKKNLINLVSNPVKKNL
                     NISGSTEYITPLHAVFEITHKCNLKCAHCYLESSPQVQDTLSLDEFIMIANNMYENGIL
                     TCEITGGEVFVHPNAKEILEFALKKFKKVGILTNGTLLKPDILDLFN"
     aSDomain        10280..10489
                     /aSDomain="Subtilosin_rSAM_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing rSAM protein in a subtilosin
                     sactipeptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_EL085_RS12420_Subtilosin_rSAM_RRE.1"
                     /evalue="2.80E-23"
                     /identifier="RREFam018.1"
                     /label="EL085_RS12420"
                     /locus_tag="EL085_RS12420"
                     /protein_end="94"
                     /protein_start="24"
                     /score="73.1"
                     /tool="antismash"
                     /translation="KNGMFKDEVFDFEYLDLNSSAFEACTYFDGSMTIIDILKDMCQKY
                     KCDINDHIEWYTNLVNDLYKKNLIN"
     gene            11010..11543
                     /locus_tag="EL085_RS12425"
                     /old_locus_tag="SAMEA3504576_02350"
     CDS             11010..11543
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: subtilosin"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SPASM"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /locus_tag="EL085_RS12425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02350"
                     /product="SPASM domain-containing protein"
                     /protein_id="WP_015728577.1"
                     /sec_met_domain="subtilosin (E-value: 1.2e-67, bitscore:
                     225.3, seeds: 6, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 9.8e-12, bitscore:
                     42.3, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 2e-09, bitscore: 35.0,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWEINDMANLVRKLGASAFSYNWIDDFGRGKKMNQLKLNKLNNLS
                     FAEFEVDVVKNNRDIIPLVPLTKNKANNCGAGWRSIVMDPNGNIRPCALFPKEFVIGNL
                     KEQSYKDVFSSEIVNRLYSLQSPQKSKFCSDECSFKDYCAGCYLKGLNSNKNHRTNYCG
                     WVKNQKLENLVEQI"
     gene            11708..12406
                     /locus_tag="EL085_RS11445"
                     /old_locus_tag="SAMEA3504576_02352"
     CDS             11708..12406
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="EL085_RS11445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02352"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_126511031.1"
                     /transl_table=11
                     /translation="MMSIHLNNLCIWYEPENLIIKNANLYLQKGKIYGLLGKNGSGKTT
                     LINTICGVIPSFSGDIIINNLNISKFNYQARLERFYVPDSPPVISQMTSKQYISFVLNM
                     YQQTLSQEHLLRISKKYNFERYLDIRMNELSFGNKKKAALICSILLDVPIKIFDEPLNG
                     LDIESIDLFVQDLFKMTKEDKCVILSSHLLDIVKKLTSNIIYINNKHCFQFTLNDESDI
                     RKVLSDYDKE"
     misc_feature    complement(12876..12888)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.7"
                     /tool="antismash"
     gene            12876..13667
                     /locus_tag="EL085_RS11450"
     CDS             12876..13667
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EL085_RS11450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_126511032.1"
                     /transl_table=11
                     /translation="MTISLLLALFLSPFFNKFISLVLLILVLLGVSIFTNFIKFNVKKR
                     KKPTKATVNEISILKWEFKKFFIEKTNVIGIISMFLFLNILNYNIESIDGMTVEFGLKY
                     FLILLIISSISPLNLMFSSDKELRKIATYMPVKKRSIFMAKILVSTLISQFFLSIVVFI
                     NEMLFQEKLGLLYFKVSILLFFLTFLRLKLDMIKPILDYEDKMELWRNPKKYLSLMIVI
                     PPLFVAVALNFYISLVIILSGCLVLELYSQLYGKEDFFGYK"
     gene            13654..14727
                     /locus_tag="EL085_RS11455"
                     /old_locus_tag="SAMEA3504576_02354"
     CDS             13654..14727
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EL085_RS11455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMEA3504576_02354"
                     /product="insulinase family protein"
                     /protein_id="WP_126511033.1"
                     /transl_table=11
                     /translation="MDINNAINIYNYHVYINKISGVENFTINQLISEILNEKFKALNDY
                     SIIPYTTTSVLGKDFYNIFYLKTLRNRYVDLDYNYWLNSFYNNFQMSDNECYLALEKLI
                     NKIKKYQLNPMNINKLLVKDKLYPNSLLGSKRLGTIETLNMNQISQELVLQRIPESIYA
                     YNLCISKIKGELNIHKLQNIKYASPFFIPHIVSQKVPPLINGQSINTICYYFESNDEYS
                     KTIYGPMLQGYLTQYGHSILFKKLRIEKFQLYHFEAHFDQETNILFIQYIVDENWKIKV
                     WNLIISEVMDLNINKEEFQTMKIFLSNEYRFKFDKSFGELNHILSLGNKFLQSNNIFKA
                     IQDIKIQDFIKFLDKKK"
     gene            14753..15709
                     /locus_tag="EL085_RS11460"
                     /old_locus_tag="SAMEA3504576_02355"
     CDS             14753..15709
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /locus_tag="EL085_RS11460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02355"
                     /product="insulinase family protein"
                     /protein_id="WP_157929458.1"
                     /sec_met_domain="Peptidase_M16 (E-value: 6.9e-08, bitscore:
                     29.7, seeds: 24, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVTKLFYADLDQNLYNLFQNEIKVTYFNKSDSYINQIKAVVNIG
                     GYQYQTGAAHFIEHLKFWKENNNLYDLMNYFGTIMNANTTEYETNFLLYCSKENTVSTL
                     TNFTSYLLNHSYTIEEFNKEKKIILNEVKGYHSILVDENEKLEYVKKIIGDENQINDIS
                     KRYLENLSSICYQKRNIHYYLIGDFSEYIHKSREISMEKIELVVNSDLIIHDYDNTELF
                     LKTLYFHINKFVNYYFQNKSLVVNFNKLYYTKKIHDIQLDDFRSYIISYIVNVYEDSDL
                     LIEFLENWKFTRGDFTKIGNLLEDQEKLSILLNEAFM"
     gene            15706..16314
                     /locus_tag="EL085_RS11465"
                     /old_locus_tag="SAMEA3504576_02356"
     CDS             15706..16314
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EL085_RS11465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMEA3504576_02356"
                     /product="hypothetical protein"
                     /protein_id="WP_037544039.1"
                     /transl_table=11
                     /translation="MKKTPIVLLFTFAIVLELSPLKSHLVKLLIKSQLLNPYSWSSFLF
                     ITLLFSIILTPIQGVFYSFNNKMQIVNSVYIKNKFKMTEEVIEISKKIKLPTKKQVFLI
                     LLINVITYDVFISILSGFKLNTPLVAFIPIGIIFMISTYFLVYRFCKQRINHKILIFLM
                     SITFSCVILSYVKEEIVIFIVIEIIVFYLYNMFLKIIKR"
     gene            complement(16776..17291)
                     /locus_tag="EL085_RS11470"
                     /old_locus_tag="SAMEA3504576_02357"
     CDS             complement(16776..17291)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166374.1"
                     /locus_tag="EL085_RS11470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02357"
                     /product="hypothetical protein"
                     /protein_id="WP_014614839.1"
                     /transl_table=11
                     /translation="MNQVTKIAVATTLSLGTLLGATAGASAIHADAHAATEQQTPYYTY
                     NGLFNFKGNKALEDKNFYRALQHDNFKYEGLKVGQSTFADVKKAVGNDVKKYYEEKGVT
                     YYEKNDVIFGIDSEGKLVNMTLLIEKINHSDKSVRDHVKQGEIYDTKTTHVAFYSGNSI
                     VIKAKESR"
     misc_feature    complement(17426..17437)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.71"
                     /tool="antismash"
     misc_feature    17463..17479
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 24.15"
                     /tool="antismash"
     gene            17596..17871
                     /locus_tag="EL085_RS11475"
                     /old_locus_tag="SAMEA3504576_02358"
     CDS             17596..17871
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728571.1"
                     /locus_tag="EL085_RS11475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02358"
                     /product="hypothetical protein"
                     /protein_id="WP_015728571.1"
                     /transl_table=11
                     /translation="MAKKSKKSLSVLSTALTGATLVVDFYNSYIYRSKNRKLSYISVGL
                     GLVDVVLSFYLSLGAKSKFTKAWSFFSASRQVWSGIQKIQAIRTRG"
     gene            complement(18258..19265)
                     /locus_tag="EL085_RS11480"
                     /old_locus_tag="SAMEA3504576_02359"
     CDS             complement(18258..19265)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498204.1"
                     /locus_tag="EL085_RS11480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02359"
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="WP_014614841.1"
                     /transl_table=11
                     /translation="MRKKFIAAVIGTTAAATALSAHADASTTYRVQPGDSLWAIATKYN
                     ITIAQLKQYNGLNSNLIFPNQVLKVSGGSSTSRTTTHHSSHSTTSSTVYTVRYGDTLSG
                     IAARYGTTYQNIMKWNGLNNFLILPGQKLNVSGPSTTPSRSTAKKPAPKTSSRNASYYT
                     VQPGDSLSLIASKYGTTYQHIMELNGLTSFLIFPGQQLKVSGSAPSRQTTPTPSPSYQS
                     PTFNHQNLYDWGQCTWYVFNKRKEMGRPISTYWWNANAWDDNAARDGYTINNQPAVGSI
                     LQSDLGYYGHVSYVERINADGSILVSEMNFTAAPGILTYRTVPAHRVSAFKYIH"
     gene            complement(19603..19884)
                     /locus_tag="EL085_RS11485"
                     /old_locus_tag="SAMEA3504576_02360"
     CDS             complement(19603..19884)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166378.1"
                     /locus_tag="EL085_RS11485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02360"
                     /product="hypothetical protein"
                     /protein_id="WP_201800462.1"
                     /transl_table=11
                     /translation="MNRIRVVYIVNVLNYLFLIVGGLICVQLMLAFGDLLLYFEATSLA
                     AGIFSLWHLNADDAKLQKVGLIWFIINLLNIFVLTPLIILVLFFGISF"
     misc_feature    complement(20931..20943)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
     misc_feature    complement(21226..21236)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
ORIGIN
        1 tgtgaaattg acccataact catcgctcct gttttaaagc gtttcacaat ttcctcaacc
       61 ggttcgactt gtgcaatatc gatggcctgt tgtgatttga aagcaaataa gtctcgaata
      121 tggctgctcg tttgatcgtt cgccacttta gaaaatgctt taaactgcgc ataatcattg
      181 tcgcgacacg catgttgaag taatcgtatc gttgttggat tgaatgtatg atgttgacct
      241 tgttgacgcc attggaatgt gcttcctggg tctaacgtgt cacctttaat cgtttggcgc
      301 gccttgtttt cagcgtctaa tgtttcaatg gagatgcccg ataatttaga aggtgtgccc
      361 gtaaaataat cgttaatcac tgcttctgat aaccctaccg cttcaaagat ttgtgcccct
      421 tgataacttt gaaccgtaga gatacccatt ttcgccatta ctttaatcag tccctctgat
      481 aaaatggcgt tgtaacgcgc gacgttatcg gctactacac cgtctaatct accgtgctct
      541 gtcaattgcg caatcgtttg ttgtgcaaga tacggaacga ctgcattcgc accgtaaccg
      601 actaaacaag caagatgatg cacttcacgt gtttcaccag aacttgcgac aatgctcgtt
      661 tgcatccgta aattttcacg aattaataat tgatgaatat gactcaccgc taataaaatc
      721 ggcatcgcat actggtctac ttcggtgacg tcactatcat tcaacacgat gatttctgcc
      781 ccttgacgta ccgctttgat gaccacttcc cctaagttgt ctagcgcttc ttttaatcca
      841 cctatgtaac ctgtcgaaaa ctctgctacc gtaaagcctt cttgtccgat gacgtctaat
      901 tgtgctggtg tcaacacggg atgtttcagt tgtacccgct tgaggacatc cggccctggt
      961 tccagcaagt tgccttcttt acctaaataa ctcaattcac tcgttacaat tttttcacga
     1021 tatgcatcaa tcggtgggtt cgttacttgt gcaaagagtt gtttgaaata attaaacagt
     1081 gattcaggtc gttcgctgag tacggcaagt ggtgcgtcgt aacccattgc cccaatagga
     1141 tctttgccac ctgtcacgag ttcagttaaa tacttgtcca actcttcttt cgtatagcca
     1201 aagcgttgtt gcattttaag aacatggtct acagcgacag gtccacccgc atatgagacc
     1261 gcttctaaat cgagcttgtg ttgatacgtt tccaaccatt ccaaataggg ttgctgctct
     1321 gcaatcgcgg tttttaattc atggttttcg ataaccttat gttgattaaa atcgacaagc
     1381 aatagcttac ctggattcag ttgcccttta aatacaacat ctgcttcgtc tacatccacg
     1441 acgccgactt ctgatgaaaa gacgatttca ttttgtttcg taattgtata acggcctggt
     1501 ctcaagccat tccgatcggt taaagcccct aacttgtcac catcacagaa tgaaatcatg
     1561 gtcggtccat cccacggctc cattaaatag ctataaaatt cgtaaaatgc gcgtactttc
     1621 ggatcgttac tttcgttata caaccacggt tctggaatga gtaacatcgc tgcttgttca
     1681 ggcgacatcg ctaaacttaa aaactctagt gcgttatcca caatagccga gtcactgccc
     1741 gattcatcca atatttgatg gactttatgt tgcgcctcgc cgaaaatcgt ttcaattaag
     1801 cgacgttgac gggcacgcat ccagttcaca ttaccttgaa tcgtattaat ctcaccgtta
     1861 tgcatgagca agcgatttgg atgtgcacgc tcccaactcg gaaatgtatt cgtactaaaa
     1921 cgcgagtgta ccgaaccaaa tttcgatacg tatgacggct gttgtaaatc gacatataat
     1981 gatttaattt ggtctgaacg caaccatcct ttataaacaa tggtacgtgt cgataaactc
     2041 gtaaaatata aatctaactg tgcactttca ccatattttt cgatttgctt acgcgcaagg
     2101 tacaatgcac gcttaaagtc ttgagcactc tcatttacga cgaagacttg ttgaatgaca
     2161 ggcatcgttt ctgctacatg cggcgcgaga cacgtcacgt cgaccggtac ttgacgatca
     2221 ccgatgatac gcagcccttc tccttcaaaa attgatgcca ttaccgctgc atgttttgtg
     2281 tcagaaatca tgacgttggc aaacaacata cctaccgcat agtcaccttc agtcggcaaa
     2341 tcaaaatcag tgacttctga gaaatattga taaggcactt ctgtcataat gcctgcccca
     2401 tcacctgtaa taccatctgc accgatacct ccacggtgat cgagacggcg taacatttct
     2461 aaagactttt cgacaatctc atgagaacgt tcattattca tattcgcata aaagccaatc
     2521 ccacaagcgt catgttcttc acgactgtca taaagtccga ttttggaatt taaataattg
     2581 ttagacattt ctgacacctc cctttagtaa tatttcaact atatagcaaa gcgatttatc
     2641 acatcaatat atacttgaga tgaaatctat ctaattttga gataatataa attgtaagag
     2701 gtggaatggt atggaattga aacaactcaa atactttgtc gaagtcgcaa aacgtgaaca
     2761 tatttctgaa gcagcactcg aattgaacgt cgcacaatct gcaattagcc gtcatattca
     2821 taatttagaa aatgaactcg aaacatcatt gttttatcgt cgcgggcgta acgtattttt
     2881 aaccgcagaa ggcaaacagt tgcttgaata tgcgcaacaa attcttgagc aagtcgatct
     2941 cactttgtcg cagtttcaaa cgcaagtaca acaacagcaa agcgtcttca caatcggtta
     3001 tgtggacggc tcaatcggcc aaattttacc gcaagtgtta cagacaattg aaaacgagct
     3061 gtccttgtca ctcattccta cattgttaga tgaacaagag acattacaag cgttgcgagc
     3121 acaagaaatt gattttgcac tgacaactgt cacaacgacg gatcctacat ttgaaatagt
     3181 gccattagta gaagaaacgt acgtcttata tggggattta cacgcaccaa tgatgaacgt
     3241 accaaatcca ccgttatcac acatattgaa acaaccgtta tacttgcttg aaccgattcc
     3301 tacagacttc aaaacgtatt tgataacaca tgccgacaaa cccgtccgtg tcttgaatca
     3361 agaacaattc gcacgcttta ttttacgcaa tcaaaaaggc ttcgtacttg caccaacgta
     3421 cgtcaactta tatagtcaaa atcaacaatg gaaaaagatt tcattgtcac atacagattt
     3481 caaaaaaacg ttacgcctca tttatcgacg cgatttacaa aaacctttac gtgatgaagt
     3541 catccaaatc atcatgacac atatacaaca acgttcggtt tatcattaag gagaatgatt
     3601 tatgaagcaa ctttcgaaaa aaataaacac cattgtcatc gtggtgagta tcatcgtagc
     3661 gatttgtgcg atagtattca cacgtttgaa tagttcgttt tatcatatcc cgctcggtga
     3721 agtgacgaaa attgaaagtc atcaccaaga aaagtcagta gatgaacaac ataatcaaga
     3781 tgtgaaatat aaagacgtgt tacaagtcaa attgttaaat acaaaacaac aaggcgacac
     3841 ccttcacgtc gaacaccggt ataacgcctc gaaaactgaa gaacaagcgt atcaaccggg
     3901 tgataaagtg ctactgcaca aaggaaaaaa tcaaaaagat acatatatta ttgagaaaaa
     3961 acgcgatacc gtactcgtga cagtcatcag cgcgttcttt attgcattac tcattgtcgg
     4021 gcaacggatc ggacttcaat ccatcctatc actcgttatt aacgcgtttg ccatcttgtt
     4081 agccattagt ttgtatcacg cgtatccgca actgaatctc tttttattaa tgagtctcgc
     4141 agtcatgatt gcgacgattt tgacactttg gctcgtcatt ggttggtcaa tgcgtacgat
     4201 tgtgaccatt gtcagcactt tactcggcac gttcatttgt atttttatcg catggagtgt
     4261 catcggttta acacacgggc aagggattaa atatgaaacg atgagctttc tcacgattca
     4321 acctagaacc gtcttcctta cttccgtcat ggttggaacg cttggagcag tgatggatgt
     4381 cgccattacg atttcaagcg ggatgtatga agtgctaaag cggtcgccac acatttcaat
     4441 gcaacggtgg gtgctcgcag gccgtaacat cggtcgtgac attatgggaa cgatgacgaa
     4501 cattttatta ttctcatatc ttgcaggaag tttaccgatg ctcttacttt atttaaaaaa
     4561 tggcaacacg ctcacttaca gcattagcat gaattggtcc ttagaaattt cccgggcaat
     4621 tacaggcggg attggcatcg ccttaactat cccattgacc atttttctca tgcgtatatg
     4681 gttaagccta cgaggaggtg caacacgatg aatgccattg tcatcttagg tttgattctt
     4741 ttcatactca tgctcatatt tggcggtaaa acaggactcg tttcattttt aacgctattt
     4801 ttaaacttta tcattttatt cattactgta ctcgccatcg tttttggcgc gcctatttat
     4861 gtcgttactt tcattttttg tattattgtc tcgatggtta acttgttttt actcaaccgc
     4921 tttaatacaa aaactttagc tgcatttatc gcgagtacgg tcacgacatt attaatgatt
     4981 gtcgccgttt acttgtccgt acattggggt catctacaag gtttcaccca agaagaacaa
     5041 gacgaaacgt atatcttctc cttaaatatt gggattgata tggaacagtt tatgattttt
     5101 actgtgctac ttgctgtcat tgcagcagtg attgacttgg cgattacgat tagctcacct
     5161 gtatttgagt tacacgagac gaacccctca ttatccgaac gcgaactgtt tcattcaggc
     5221 atgcgcgtcg gccgagaaat tttagcgacg tctgcaaata cgatttatct cgcattgatt
     5281 gggggttcaa tgacaattgt tttttggttt ttcaacttgc attacagttt cggccatatg
     5341 ctcaatgcca aattatttac acaagaagtc attacaattg ctttaggagg ggtcgccatt
     5401 gcagtctgta ttccgattac ggctatgata actgcttggc ttgtgaaaca tcgccatcaa
     5461 cttccttttc atctagactg agcttcaaga cgagtgaaaa cgggtacaat gtgaacgagg
     5521 tgaatttata tgacagcgat tcatattcat ggcgcacgtc aaaacaactt aaaaaatatt
     5581 gacgtctcca ttccgaaaca tcagttaacg gtcgtgactg ggcgttcagg atctggcaag
     5641 tcttccctcg tttttaatac gattgccgct gaatccgaac gactattgaa cgaaacctat
     5701 tcgagttata ttcagcacca attaacgcaa tatgaaaaac cggatgttga ccatattgaa
     5761 aacttgcccg tcgcgatggt catcaatcaa aaaagactcg gtggcaattc gcgctcaacg
     5821 gtcggtacca tttcagacat ttatgcgtcc gtccggctat tgtggtcgcg tattggcaca
     5881 cctttcgtcg gttattcaga cgtcttttcg ttcaataatc cgaatggaat gtgtgaacat
     5941 tgtcaagggc ttggttatgt tgaagatatc gacttgaatg aactgctcga tttcgacaaa
     6001 tctttaaatg aaggtgcgat tcgctttccg tcatttaagc cggatagttg gcgcgggaaa
     6061 cgttatcgct attcgggttt gtttgataat gataaaaagc tcaaagacta tacaaaagaa
     6121 gagctcgata cgtttcttta taccgagcca acacgcttga aaaatccacc ctcagaatgg
     6181 cctaaaactg caaaatttga agggttaatc caccgtttcc gacgctcatt tttaattaat
     6241 gataactttg aaaagaaacg ctttttaaag gacgttgaac gcgtagtcac caaacaaaca
     6301 tgtcctgtct gtcacggaca acgcttaaat caaaaagtgc tcagctgcaa aattcatgga
     6361 ctgaacattg ccgattttac agcactgacg attgaagaaa cgctcccatt tttagagcaa
     6421 atcgacagcg acaaagcgac ttatattatc gaaccattga aagcccaact ccaagctttg
     6481 aatgatattg ggctgaacta tttaacgctc gcacgtgaaa caacgaccct ttctggcgga
     6541 gagtcacaac ggattaagct tattcgtcat ttgaacagtc cgttaaccga tctcgtgtac
     6601 attatcgacg aaccgagtgt cggtttacat ccagcagata ttgaaaaaat caacgaaatc
     6661 atgctcaata tccgtgacaa aggtaatacc gtcatcatcg ttgaacacga tcccgacgtc
     6721 atcaaaattg cggatcacgt gattgacatc ggacctggcg cagggaaaca tggcggccag
     6781 attacatttg aaggcagtta tgcacaactt aaacagtccg ataccgatac agcgcgcgca
     6841 ttgacacgtc aacatcactt gaaacagtca gcgattcgtg acaaccaagc atggttacac
     6901 ttagaacatt tgcaacacaa caacttacaa gatgtgtctg ttgcgatccc gaaaaatgcg
     6961 caaacggttg taacaggtgt cgctggttca ggaaagagct ccttaattaa aggcggtttt
     7021 gcgaaacatg aagatgcgat actcattgat caaaaaggcg tacacacttc caaccgatcg
     7081 aatttattaa cgtacacggg tattttcgat gatattcgtg agtttttcag taaagcaaca
     7141 ggattgaaaa aaagtatgtt cagttataac tcagatggcg cgtgtccaaa ttgtaacggg
     7201 aaaggcgtgc tcaaaaccga actcgccttc atgccagact tctcgcaagt atgcgaggtg
     7261 tgcggaggga cccgctatcg tccagaagtg ttggaaacga aagtcgacgg ttattctatc
     7321 gcggacgtgt tagcactcac cgttgaagaa ggcttgtcac ttttccaaac acatgacgcc
     7381 attgctcaaa cgttaagcgc cttgcaatcg acaggactcg gctatatgac actcggccaa
     7441 tcactcgaca ccttatctgg cggggaaatt caacgactca aactgacgcg ctacatgatt
     7501 gagcctgtta cagaaaagat atttattttc gatgaaccga caacaggttt acatgaagat
     7561 gatatcccaa ttttacaggc acgcttcgat caacttattg aagaaggcca taccgtcatc
     7621 ttaattgaac acaatatcac gatgatgaca cgagcagact ggctcattga cgttggacct
     7681 ggtgcgggaa atcaaggggg acgcattctt tatagtggac cacctgcagg cctaaaaaac
     7741 gtcgccgaat ctcgcaccgc tccccattta tttggtagtt tatcggaata agtggtacat
     7801 tgagtatggg gctaggacat agaccttcag ctcctttaag aaaaaaaacg tcaaaattca
     7861 atttttagaa tttgatggct tttttcttat gagcgaacga aaacgagcac tgattttggc
     7921 cagttttcgt atatttactg ccatgagtgc gatgccaagt tcacactcaa cttttgattg
     7981 ggtgcgaacc gataatcttt ggaaacccaa attagctttc agatttacaa aagttgattc
     8041 cacatctatc tttctctttt gataaatgtg cttcgttttt ggatctgaaa gctgcttatt
     8101 tatgaaggct ttaaaataat cccaagttag atttttaaat aaacttttat ttgtgttaga
     8161 ctttgtactt tgcttcatac attaacttcg taatggacag cccacacacg cttcacatgc
     8221 atataattta aaatctcttt gaataccgta tccatctctt ctctttctgt aactctggaa
     8281 atgtaatgtc ttcttattcg gacatatgta ataatcttct atcacgttgt attcccagtt
     8341 tgcagtaata aatggattgt ttttatattt acgctgcttc tctttgaggt acatactata
     8401 agtgattaaa ggtgttttct caaattcatc gagtatcacg gtatagttgt attcacttcc
     8461 gtaacctgca tctgccacga tatactctgg aatgtcacca tataatttgt gaattgattt
     8521 taaaaatggt ttgagcgttc ttgtatcacc tggataacta taaacaccaa aagctaaaac
     8581 gaattgatta ttggtagcta tctgtagatt gtaacccggt ttcaactcac cgtttctcat
     8641 atggtcatct ttcattctca tgaaagtcgc atcataatca gtcttagaat aactttttct
     8701 gtcgccataa atttccattt gcttgtcata ttttaattta cggtccttaa aatcttggat
     8761 agccttctta ctttgtctca cgttacttct ttcttttcta aggttttttc ttgtctctac
     8821 atcttgagtt gtttcaattt cagacgtgag cgcatcattt ttatgaccta aatgcgtctc
     8881 tatttgattc agttcttcac ttgttaattc atgtccagat tcacgcttga tttcaggaat
     8941 aatctcttca gaaacgagtt gttcatataa tgccgttgat ttttcaatga cagactgact
     9001 aaaacgcttc gtattagcca gccactgaaa tgtatactta ttcgcattgg cttctatttt
     9061 tgtgccatca atataaatga catcctctgt aatgagttgt tcttctaata actgtgctct
     9121 taaaccgaat attattacac ttcaattatt aacctcttcc ttaaataatt tattattcac
     9181 atctcacaac tttagctttt aaattcttcc tttcaggcta tagtttatca aaaaagaatc
     9241 aaaaagtttt gcaaacgaag ctattacatc cttcacatgg gatcatttgt tttatctctt
     9301 ctaaagataa tatttttata aagcgagaag acgtatctac aatttttcta tcttttaatt
     9361 gcttcataat tctagcgata ttctctctcg tagttccaca atattgagct aattcttctt
     9421 gagtgatttt aatttttatc acaatcattc tcccttcttc ttctccaaat gtattagcta
     9481 atctaactag aatagatagt aatctacaca ctttgtcttt agttaacata tctctaataa
     9541 acatatcttt tttcacattt tcatttatga gtaattttat gtattctcta caaattttct
     9601 catcatttat tatgctgctt ataagcattt tataaaatac aacttctgtt ttcccacaaa
     9661 agtatttact attatcacaa atgaacaaac tatctggccc tatgatttta taaatcaaag
     9721 tagtaccatc tatgttatct tgatgtacat ataatatacc cttatctact agatatatat
     9781 atttttcaaa attctcttga aaatactccc ccttacacaa cttattattt atcccttttt
     9841 ctttgataat ctctttaatc cctagcaact ctaccataac aaccccccct tataaaaaat
     9901 tataccattt aaattacttt tagtaacatg cgttacaaaa cttttgaata atttatatta
     9961 aaatgtgttt gtagttaaat aaaataaagg ggataccaaa atggaaaaag gcattatggt
    10021 aagtaacaag ggttgttcga cttgttcaat tggagctgct tgtcttattg atgggccatt
    10081 acctgacttc gaagtcatgg gaattactgg aatttttgga ctaacttctt agcactgttt
    10141 gtatgccggc aaaatgatag gatttatcat agtttgtcgg cgtacttttt attaaggggg
    10201 gatagaaatg aatcaatata tattttttaa tgacaatgct aggttgcatc aattacctca
    10261 tggtggagta attgaggtga aaaatggcat gtttaaagat gaagtgttcg actttgaata
    10321 tttagattta aattcttctg ctttcgaggc atgtacgtat tttgatggtt ccatgacaat
    10381 aatagatatt ttaaaagata tgtgccaaaa atataaatgt gatattaacg atcacataga
    10441 atggtataca aatctagtta acgatttgta taagaaaaat cttattaatc tcgttagtaa
    10501 tcctgttaaa aaaaatctta atattagcgg atcaactgaa tatattacac cactacatgc
    10561 ggtgtttgaa attacacata aatgtaattt aaaatgcgca cattgttatc tagagtcttc
    10621 acctcaagtt caagatacgc tttcattaga tgaatttata atgattgcaa ataatatgta
    10681 tgaaaatgga attctaacat gtgagatcac tggtggagaa gtgtttgtac atccaaacgc
    10741 aaaagaaata ttagaatttg cactcaaaaa gttcaaaaaa gtcggtatct taacaaacgg
    10801 aacactgtta aaaccagata ttctcgattt atttaattaa atataagaaa aaaattgtta
    10861 tagggatttc attagatagt gttaaccctg aaaagcacga taattttcgt ggcaaaaaaa
    10921 attcatttaa tcagacttgt aaaaccatta aaaaactttc tgaaaatggg ctttttgtaa
    10981 gggtcggaat gtctatttat gaggataata tgtgggaaat caatgatatg gcgaatttag
    11041 taagaaaact aggagcttca gccttttctt ataactggat agatgacttt ggtagaggta
    11101 aaaaaatgaa tcagttgaaa ttgaataaac taaacaactt gtcttttgct gaatttgaag
    11161 tggacgttgt taaaaataat agggacatta taccattagt tcctctaact aaaaacaaag
    11221 ccaataattg tggggctggc tggcgttcaa tagtcatgga tcctaatgga aatattcggc
    11281 cgtgtgcact atttccaaaa gaatttgtaa tcggtaactt aaaagaacag tcatataaag
    11341 acgttttctc ctcagaaata gtaaatcgat tatactctct gcagtctcca caaaaaagta
    11401 aattttgcag tgatgaatgc agcttcaaag actactgcgc tggctgttac ttaaaaggtc
    11461 ttaatagtaa caaaaaccat cggacgaatt actgcggttg ggttaaaaac caaaaattag
    11521 aaaacttagt agaacaaata taaaaggaga taaaacatga aaagagactt aattacaata
    11581 accattgcta taattttttt aatatctagt gtaatattcg ccattaagca agaatgggtt
    11641 ttcgcagtaa tcttcatatt gatagggatt atctatatat taaaaacatt taaaagaggt
    11701 aaatctaatg atgagcattc atctaaataa cttatgtatt tggtatgagc cagaaaattt
    11761 aataattaaa aatgcaaatc tatacttaca aaaaggaaaa atttatggat tgcttggaaa
    11821 gaatggatct ggtaaaacaa cgctaataaa tactatatgc ggggttatac caagtttttc
    11881 aggagatata atcattaaca atttgaatat atcaaaattt aattatcaag ctaggttaga
    11941 aagattttat gtaccagata gtccccctgt tatttctcaa atgacaagta aacaatatat
    12001 aagttttgtt ttaaatatgt atcaacaaac tttaagccaa gagcatttat tgagaatttc
    12061 taaaaaatat aattttgaaa ggtatttaga tataagaatg aatgagctct cttttggaaa
    12121 taaaaaaaag gctgctttaa tttgttcaat cttattagat gtacctatca aaatattcga
    12181 cgaaccatta aatggattag atatagagtc aatagatttg tttgttcaag atcttttcaa
    12241 aatgacaaaa gaagataaat gcgtcatcct atcttctcat ttattagata ttgtaaaaaa
    12301 gctgacatct aatattatat atattaataa taaacattgt tttcaattca cgcttaacga
    12361 tgaaagcgat ataagaaagg ttctatctga ttatgataaa gaataatgga tatttaattc
    12421 tactctattg ctattactac aataaactta atctaagagc cataattaga aattcactag
    12481 gaatagcaat tcttttaata tttgaataca aattgattga aagtcatttg gataatgtca
    12541 atttgttata ttccaattat ctaagattat ctatcatatt ctcctcaatt atattgacta
    12601 atatttactg gaactctttc cgcgaaagcg agttatatag actcttaggg tacatgccta
    12661 ttaataatgt aaaggtttta ataattctaa atgtatatat tttcgttgag ataaccggga
    12721 gaagacttgt ttttttactt gtactaccat tttatgtttt gctaatcggc aaattcagta
    12781 tttacgggag cattttgaca ttttcattat tcatactact gattgtatct tctcaactta
    12841 tttgcattct tttgcatgaa tgtgaaaaga gacatgtgac aatttcatta cttctagcac
    12901 tttttttaag cccatttttt aataaattta taagtttggt gcttttaatt cttgtattat
    12961 taggtgtatc catctttact aattttataa aatttaatgt aaaaaagaga aaaaaaccta
    13021 caaaagctac agtaaacgaa atatcgattt taaaatggga gtttaaaaag tttttcatag
    13081 aaaaaacaaa tgtcattgga ataataagta tgttcctttt cctcaatatt ttaaactaca
    13141 atattgaaag tatagatgga atgacggtag agtttggact aaagtatttt ttgatattac
    13201 taattatttc ttcaataagt ccattaaatt taatgttttc atctgataaa gaattaagaa
    13261 aaattgctac ttatatgcct gttaaaaagc gaagtatctt tatggctaaa attttagttt
    13321 caacattaat atcacagttt tttctttcta ttgtagtgtt cataaacgaa atgctattcc
    13381 aagaaaagtt aggcttacta tacttcaaag tatctatttt gttgtttttt ttaacttttt
    13441 tgagattaaa acttgatatg ataaagccca ttttagatta tgaagataaa atggaattgt
    13501 ggagaaatcc gaaaaaatac ttatcattga tgatagtgat ccctcccctt tttgtagctg
    13561 tagcactaaa tttttatatt tctctagtta ttatattatc aggttgttta gtattagaac
    13621 tatatagtca actttatgga aaggaagatt tttttggata taaataacgc tataaatatt
    13681 tataactatc acgtctatat caataaaata agtggcgttg aaaatttcac tattaatcaa
    13741 ttgatttctg aaatattaaa tgagaaattc aaagcattaa atgattattc aattattcct
    13801 tatactacaa caagtgtttt gggaaaagac ttttataata ttttttatct aaaaacttta
    13861 agaaaccggt atgtagacct agattataac tattggctca atagttttta taataatttt
    13921 caaatgtctg acaatgagtg ttacttagcc ttggaaaaac ttattaataa aattaaaaaa
    13981 tatcagttaa atccaatgaa tatcaataaa ttattagtaa aagacaaact ttacccaaat
    14041 tcattattag gaagtaaacg tttagggact attgaaacat taaatatgaa tcaaataagt
    14101 caagagttag tactacaaag aatacccgaa tctatatatg catataattt atgcatctca
    14161 aaaattaaag gagagctaaa tatacataaa ttacagaata ttaaatatgc atctcctttt
    14221 tttataccac atatagtaag ccaaaaagtt ccaccattaa taaatggaca aagtattaac
    14281 acaatttgtt attattttga aagtaatgat gaatattcaa aaacaattta tggtcctatg
    14341 ttacaaggct acttgacgca atatggccat tcaatattgt ttaaaaagct aaggatagaa
    14401 aaatttcaat tatatcattt tgaagctcat tttgatcaag aaactaatat cctattcatc
    14461 caatacatag tggatgaaaa ttggaaaata aaagtttgga atttaattat atctgaagtt
    14521 atggatttaa atattaataa agaagagttc cagactatga aaatcttcct atcaaatgaa
    14581 tacagattta aattcgataa atcatttgga gaattgaatc acattcttag tttaggaaat
    14641 aaatttttac aatcaaataa tatatttaaa gctatccaag atataaaaat tcaagatttc
    14701 atcaaatttc tagataaaaa aaaatgataa atattgtata tgagggatga aaatgaaagt
    14761 tacaaaacta ttttatgcag atttagatca aaatctttac aatctatttc aaaacgaaat
    14821 aaaagtaaca tactttaata agagtgactc atatataaat caaattaaag cagtcgtaaa
    14881 tatcggggga tatcaatatc aaaccggagc tgctcatttt atagagcacc taaaattttg
    14941 gaaagaaaac aataacttat atgatttaat gaattacttt ggcactataa tgaacgcaaa
    15001 tactacagaa tatgaaacta attttttact gtactgtagt aaggaaaata cggtttctac
    15061 tttgactaat tttacatcat acttattaaa tcacagctac actattgaag aatttaataa
    15121 agagaagaaa ataatattaa atgaggttaa aggataccat tcaattttag tggatgaaaa
    15181 tgaaaaatta gaatatgtaa aaaagataat aggcgatgaa aatcaaatta atgacatttc
    15241 taagagatat ctagaaaacc tatcttcaat atgctatcaa aaaagaaata ttcactatta
    15301 tttaataggt gatttttctg aatatatcca taaaagcaga gaaataagca tggaaaagat
    15361 agagctagta gtaaacagcg atcttattat acatgattat gataatactg aattgttttt
    15421 gaaaaccctt tatttccata taaataaatt cgtaaattat tatttccaga acaaaagtct
    15481 tgtggttaat ttcaataaat tatattatac caaaaaaatt catgatattc agttagatga
    15541 ttttagaagt tatataattt cttatatagt aaatgtttat gaagattctg atttgttaat
    15601 tgagtttttg gaaaactgga aattcacaag gggagatttt acaaaaatcg gtaatttact
    15661 tgaagatcaa gaaaagttat ctatattatt aaatgaggca ttcatgtgaa aaaaacacct
    15721 attgtattat tattcacttt cgcgatagta ttagaattat ctccccttaa aagtcattta
    15781 gtgaaattat tgataaagtc ccaattatta aatccgtatt cttggtctag ttttcttttt
    15841 attacactgc ttttttcaat tattttaacc ccaattcagg gtgtttttta tagttttaat
    15901 aataaaatgc aaatagttaa tagtgtatat ataaaaaata agtttaagat gaccgaggaa
    15961 gtaatagaaa ttagtaaaaa aataaaatta cctaccaaga aacaagtgtt tttaattctc
    16021 ctaataaatg taataacgta tgatgtcttt attagtatcc tctcaggatt taaattaaac
    16081 acacctctcg ttgcatttat tcctatcggt attattttta tgatttctac ttatttttta
    16141 gtgtatagat tttgtaaaca aagaataaat cataaaatcc tgattttttt aatgtcaatc
    16201 accttttctt gcgttattct atcatatgtg aaagaagaga ttgtgatatt tatagtaata
    16261 gagattattg ttttttatct ttataatatg tttttaaaaa ttattaaaag ataattacaa
    16321 agaactactc tgtatataac ttttcagtaa aactaattac attgactgta ggcaacgtta
    16381 caatgatact gttaataatt ttacaaatac atttaaactg aatcttacag acgttatagt
    16441 tgtccctaaa atccttactc atcatttata aatagcgatt ttcattaaac tttaataaga
    16501 taatccttgt aaaaactccc tttatagggg ggttgttgca caattgtttt gattattaat
    16561 atagaaacac tgtatttctt acaatcgcct gcaaatacac agcgtctcgc caagtctatt
    16621 gcagaagctg aacaaagcca cacgattgca ttaaattgaa atacttaaac aagctctcat
    16681 caactcaaaa actccgagac ttatcgtcta ctattcacga aaagtctcgg agttttttta
    16741 actatgacgt tgtatgggca aagtacgcct caatcttaac ggctctcttt tgctttaatc
    16801 acaattgagt tgcctgagta aaatgcgaca tgtgttgttt ttgtatcata aatttcgcct
    16861 tgtttcacat gatcacgcac agatttatca ctgtgattaa ttttttcaat taataatgtc
    16921 atgttgacca atttaccttc gctatcaata ccgaaaatga catcattttt ctcgtagtaa
    16981 gtgacacctt tttcttcata atattttttc acgtcgttcc ctaccgcttt tttaacatca
    17041 gcgaatgtag actggccgac ttttaaacct tcatatttaa agttatcgtg ttgtaatgcg
    17101 cggtaaaagt ttttgtcctc taaagcttta ttccctttga agttaaataa gccgttatat
    17161 gtgtaataag gtgtttgctg ttccgttgcg gcatgtgcat ctgcatggat tgcacttgcc
    17221 ccagctgttg cccctaataa tgtgcctaat gataaagttg ttgcgactgc aatttttgta
    17281 acttgattca tgtgtaccac tcctctttct ctcgatactg ttctaaaaat taggaattta
    17341 tgttatttgt aattgtcgaa ttaaatttcc aatttataat aacttatata tcaaattgtt
    17401 ccttactcga atgataacaa attaagataa cattttcatc caaaagtttt tcggtcattg
    17461 gactgaaatt tttctacaac gctgtatcta aaagacgtgt taacaatcat ttaaggaaag
    17521 tgcatatgtt gggttttgac ctatcagtaa tgttataatg aatgacatca tactatattc
    17581 attgggggaa gtataatggc taaaaaatct aaaaaatcat taagtgtgct ttcaactgct
    17641 ttaacaggcg caacattagt tgtagacttt tacaattctt acatttatcg ctcaaaaaat
    17701 cgcaagctaa gctatatttc agttgggctt ggcttagtag atgtcgtatt aagcttctat
    17761 ttatcacttg gtgcaaaaag caaattcaca aaagcatggt ctttcttctc agcaagtcgc
    17821 caagtatgga gtggtatcca aaaaatccaa gcgattcgca cacgtgggta gggtaacgac
    17881 ttgattcatt ttcaaatcat taaatatagg cgtgaacatg aaacattatg atttctgttc
    17941 acgccttttt atgtagtaac tagtaaccat tgcccagatg ctcaagtcgt tatatcttcg
    18001 cttcatccaa gtttttaaag ctaatcatta aagcatccgt ttctgtgaat atgacgatac
    18061 aaatggattg actcaacggc tttatcctta cctaagaatc atcgacaaga tgtttgttac
    18121 cattttgtta atagaatatg acatgcattt caaacttttt ggggattttt tcaaatgacg
    18181 aaaattttct cattcgaccc ttgtcttaaa aaataaaaac gtgagcacca tgtaaaaggt
    18241 atactcacgt tttttgatta atggatatat ttgaaggcac ttacacgatg cgctggtaca
    18301 gtacggtaag tgagaatgcc tggtgctgca gtgaaattca tttctgatac gagtatacta
    18361 ccatcagcat taattcgctc tacatacgaa acatgaccat agtaacctaa gtcagattgt
    18421 aagattgatc ccactgcggg ttggttatta attgtataac catcacgtgc agcgttatca
    18481 tcccaagcat ttgcattcca ccagtatgta ctgattgggc gccccatctc tttacgctta
    18541 ttaaatacgt accatgtgca ttgaccccaa tcgtataagt tttgatggtt gaatgtagga
    18601 gattgataac ttggacttgg tgtcggtgtt gtttgacgtg acggtgccga tccactcact
    18661 tttaattgtt gacctgggaa aattaagaag gatgttaatc catttaactc cataatatgt
    18721 tgatatgttg taccatattt agacgcaatc aacgacagtg aatcacctgg ttgtactgta
    18781 taataactcg catttcttga agacgtttta ggtgccggct tcttcgctgt ggaacgactt
    18841 ggtgttgtag acggaccact tacatttaac ttttgacctg gtaaaatcaa gaagttgttc
    18901 aaaccgttcc atttcataat gttttggtac gttgttccat atcgcgctgc aatgcctgat
    18961 aaagtgtctc cgtaacgtac cgtatagact gttgatgacg tagtgctatg gcttgaatga
    19021 tgagttgttg tgcgtgatgt actgctacca ccagaaacct tcaacacttg attcggaaag
    19081 attaagttag agtttaatcc attatattgt ttaagttgtg ctattgtgat gttatacttt
    19141 gttgcaatag cccaaagtga atcacctggt tgtacacgat acgttgttga agcgtctgca
    19201 tgtgctgata atgctgtggc agctgctgtt gttcctatta cggctgcgat aaacttttta
    19261 cgcattcgtt tccctcctta taaaatagaa gatattattc ttatacagtt ttatattata
    19321 caccgattat catgtagaaa tatgtctgtt atataacatt atgattacaa aaaccatcat
    19381 ggcggtcttc agaaagtaaa tatatttaca catgcttatt tgagaattaa aataatagct
    19441 atcatattat tcataaagac atttttctcg gatatttctt ttatttatct ttcgtaattg
    19501 taaaatattt gaccacttca tcattgttta tggaagtaaa cttatgcatc acgataaagc
    19561 ttgatacgac ctttttaaca ttacaaggtg cgatttcggt cgttaaaaac ttatgccaaa
    19621 aaataatacc aagatgatta aaggtgtcaa aacaaatata ttaagtaaat tgatgataaa
    19681 ccaaattaag cctacttttt gtaacttcgc atcgtctgcg tttaggtgcc acaatgaaaa
    19741 tatgcccgca gctaatgacg ttgcctcaaa atataataat aaatcaccaa acgctaacat
    19801 caattgcaca caaatcagtc cacctactat taaaaataag taattcaaaa cattcacgat
    19861 atacacgact ctgatcctat tcatttatca cgactcctgt tgaaagtcct taatatggtt
    19921 tttcatcata ctataaacct aggaaacaat tctgtaactt atcaaaatta taacattttc
    19981 atatgattgt gattatatat attagtttca tacttataaa aaagtgcgtg catgaagata
    20041 ggacataaaa ctttttggcg ctactgatac cgcattgttt taacttgtac gcttccagga
    20101 cttgtcttaa ctaaatccag tgtccacgag ggccattttc atttgttgcg tatatcaaaa
    20161 aagtcatgcc atgttcaaaa cgagacatgg catgacgctg tcgaatgtga ttgattttat
    20221 aaacgaaatt cataaggatt tgaggaagac tttaactttg gtactagcta ctgtttaacg
    20281 cagtagctgt ctgacttctc aatgcttgta cttgtgagaa gccgagtcag ccttgcgcag
    20341 gcatttacga cgaaattttt gttgcacaag aggtttctgt acgactccct caaccccatg
    20401 tcacttacaa ttgatcaagt gcttgtttta aatcttctac aagatcatct gtatcttcaa
    20461 tacccaatga taatctgatt aaaccatctg caatgccttc ttttgcacga atatcagccg
    20521 gaatcgatgc atgtgtcatt aagcttggta cagaaattaa actttcgact gcacctaaac
    20581 tttctgcgag tgtgaaatat tttgtttgtc tcactaggaa tttcgctttt tctacatctg
    20641 ctacttcaaa tgagatgatg cctgttgtgc catctgcttg cgcttgatgc acgtcgtagt
    20701 ttaaatgtga cggttggctt gggtgataca ctttcgtcac tttgtcatgt tgattcaaca
    20761 tttcgacgac cgctaatgca ttacgttcga tttgttccat acgtaaagct aatgttttca
    20821 tcccgcgaat aagcaagtag ctatcttgag gccctaatac gccccctgtt gagttttgga
    20881 cgaaagcaat gcgctctgcg agtgtttcgt ctttaactgc gacaagtccg gctacaacgt
    20941 cactatgccc acctaaatat ttcgtcgctg aatgtacaac aatatctgcg ccaagagaca
    21001 acggtgtttg ataatatggc gtcataaatg tgttatccac gacagtcatg agttcgtgtt
    21061 gcttgccgat ttcactcacc gcttggatat ctgtaatgcg cagcaatgga ttagatggcg
    21121 tttcgataaa taacattttc gtattatctt taatcttatc ttcaatattt tgaacatctg
    21181 tcgtgttaac aaagtcaaat tcaataccaa aacgtgtgaa gacacgtgtc attgcacgat
    21241 aagagccgcc atacacatca gagccaacaa taatgtggtc gccttggtct aataacatga
    21301 taactgcgct gatggccgcc atgcctgaac cgaatgcgaa accgtagttc ccatgctcta
    21361 aatcagcaat gacagactcg agcgatgaac gtgtggggtt cgctgtacgt gagtattcaa
    21421 acccttgtct taactcccca atcgcatctt gttcatacgt actcgtttga taaatcggcg
    21481 tcgtcactgc acctgtataa tcatctgtag tattaccgca atgaattaat aatgtttttt
    21541 tat
//
