LOCUS       NZ_LR134267            11962 bp    DNA     linear   CON 15-APR-2024
DEFINITION  Staphylococcus pseudintermedius strain NCTC5661 chromosome 1.
ACCESSION   NZ_LR134267
VERSION     NZ_LR134267.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus pseudintermedius
  ORGANISM  Staphylococcus pseudintermedius
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus; Staphylococcus intermedius group.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2019) WELLCOME SANGER INSTITUTE, Pathogen
            Informatics, Wellcome Sanger Institute, CB10 1SA, United Kingdom
REFERENCE   2
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2018) WTSI, Pathogen Informatics, Wellcome Trust
            Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900636355.2-RS_2024_04_15
            Annotation Date                   :: 04/15/2024 03:28:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,432
            CDSs (total)                      :: 2,350
            Genes (coding)                    :: 2,313
            CDSs (with protein)               :: 2,313
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 12 of 37
            Pseudo Genes (incomplete)         :: 27 of 37
            Pseudo Genes (internal stop)      :: 6 of 37
            Pseudo Genes (multiple problems)  :: 8 of 37
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:45:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2090876
            Orig. end                         :: >2102838
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR134267.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..>11962
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..>11962
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..>11962
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:>6962](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..>6962)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(249..2030)
                     /gene="recQ"
                     /locus_tag="EL085_RS09785"
                     /old_locus_tag="SAMEA3504576_02013"
     CDS             complement(249..2030)
                     /EC_number="3.6.4.12"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recQ"
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016424488.1"
                     /locus_tag="EL085_RS09785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02013"
                     /product="DNA helicase RecQ"
                     /protein_id="WP_015728744.1"
                     /transl_table=11
                     /translation="MEQTLSHYFGYKSFRPGQKEIIERVLNHHHTLGVLPTGGGKSLCY
                     QVPGLMFKGTTIVISPLISLMKDQVDQLTAMGISAAYLNSSLSTQAQKEIETQLVNGEL
                     KFLYIAPERFEQTYFEMLLRRVDISLVAFDEAHCISKWGHDFRPSYQAVIQRVLTMPQQ
                     FSIVALTATATTEVQQDIMERLMISRQHLVKTSIRRRNLKFQVNGTYQRQKFVLDYIQK
                     HSQKAGIVYCSTRKQVEALYEALEDADIPVSYYHAGMTAKQRDEAQNDFLYDRTRVVVA
                     TNAFGMGIDKSNVRYVIHYNMPGDLESYYQEAGRAGRDGLDSDCILLYSDRDIALHQYF
                     IGASKADDDYKERMGEKLTKMIQYTKTTKCLEATLVHYFEPNEKLEECHQCSNCIDKNK
                     TYNMTKEAKMIISGVARLRQQEGYQTVIQVLRGEVSDYIRHQGYENLSTYGLMKDYTTG
                     ELHHLIDELRFKGFLNEHDEILMCDASVEKLLSEDTQVFTRPFKRKSKETVHINTVEGV
                     DRTLFEKLVEVRREMSERLNMPPTNIFTDYTLEAFAKRKPVTKQEMIQIDGVGSYKLKH
                     YCPEFLEAIQEYKMHRS"
     gene            complement(2040..3917)
                     /locus_tag="EL085_RS09790"
                     /old_locus_tag="SAMEA3504576_02014"
     CDS             complement(2040..3917)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166146.1"
                     /locus_tag="EL085_RS09790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02014"
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="WP_100006802.1"
                     /transl_table=11
                     /translation="MEAYKIERLNKSYADKVIFDDLQLSISNQERIGLVGINGTGKSSL
                     LKVIAGMDEDFDAVQSHPKQYRIRYAAQNHNLEPTLTVYEAVYSADSKELQVIKRYEEA
                     TAQYAEQQTDKAFDAMMAAQADMDAYSAWDYSAEIKTILSKLGIHDTTRRMSELSGGQQ
                     KRVVLARTLIEKPDLLLLDEPTNHLDFESIHWLIQFVKSYPKTVVFVTHDRYFLNEVAT
                     RIVELRNGKLDTYPGNYEAYIAMRAEREEIEVRQQTKQRALFKQELAWMRAGVKARTTK
                     QQARQDRFHDLKEQVQSHQVQGKGSLNLAYSRLGKQVFELENVTKRIDGRTLFENVSTL
                     IQKGVQIGIVGPNGVGKTTMLNILAGEDQHFEGVLKIGQTVKVAYFKQTDERLNRDVRV
                     IDFLREESELARQKDGTTVSITQLLERFLFPSVTHGKKVYKLSGGEQKRLYLLKLLVHQ
                     PNVLLLDEPTNDLDTETLTILEDYIQTFGGTVITVSHDRYFLDKVVDCYWYIHDGQIDM
                     ILGQFEDYLAYQKQLEQAATQVASVKQQPTLQPKKKKGLSYNEKREYDMLLVRLEETES
                     RLAEIDAEMIEANADYAKIKALNEEKETLEAQYDTDMTRWSELEEILEQ"
     gene            4029..4844
                     /locus_tag="EL085_RS09795"
                     /old_locus_tag="SAMEA3504576_02015"
     CDS             4029..4844
                     /GO_function="GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019168536.1"
                     /locus_tag="EL085_RS09795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02015"
                     /product="ZIP family metal transporter"
                     /protein_id="WP_015728742.1"
                     /transl_table=11
                     /translation="MLDYISNMPPFLQALLAGVITWLLTALGAATVFVFKSVNEKVLNS
                     MQGFAAGIMIAASFWSLLQPAIESSSGSTVPWLPAAIGFLLGGVFIRSLDYVIPHMHRN
                     ATDESQHQEGVETSLNKNTLLLLAITLHNIPEGLAIGVAFGGIATGNEHATLLGALGLA
                     IGIGIQNIPEGAALSLPIHASGQSKWKAFNYGQASALVEPVFATVGAAAVLVVTPILPY
                     ALAFAAGAMIFVVVEELIPDSQAGKNTDLATLSLMVGFTIMMILDVALG"
     gene            complement(5001..>6962)
                     /locus_tag="EL085_RS09800"
                     /old_locus_tag="SAMEA3504576_02016"
                     /pseudo=""
     CDS             complement(5001..>6962)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /locus_tag="EL085_RS09800"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02016"
                     /product="peptide cleavage/export ABC transporter"
                     /pseudo=""
                     /sec_met_domain="TIGR01193 (E-value: 4.7e-230, bitscore:
                     762.8, seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_C39 (E-value: 7.4e-10, bitscore:
                     35.9, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="HFHYYVVIRCDKKYIYIADPVVNLTKMTYREFKEEWTGVTILLKP
                     TSLYKPIKQDKVRLTSFIPLLLKEKRLIIKIIIISILVNMINILGSYFMKSIIDFYIPQ
                     KLKTWVSMISMALIASYIMQQILSYLQKYFLILLVKTYSINILSPYIKHLFRLPITFFE
                     TGRAGDILSRLNDTNSITDALASTMLSLFLDVTIIVGISIFLYYQSTILFVLILIIIPI
                     YAVIVFGFMNSFERKRIKSMEANATLSSYLMEDITGMETIKALTCEKKCYKQIKYNFSN
                     YYEKTFLLNKLNIKQNVLKKVVQLILNVSILWVGANLVIDNQISIGTLLVFNSLMIYFM
                     NPIENIMNLQLKLQIAGVANNGLNEIFTVKSEFEKNKLDIKLNQNSSKLIFSNISYGYV
                     YEKEILTDINITIEHNSKVAFIGESGSGKTTLAKLMVSFFEPSKGKITLGGLDITNINK
                     SVLRQQIIYIPQQTYIFNGTILENLLLGVVKEVTQKDIDSAIKMAELKSFIDSLPLGLD
                     SVLSSDFSALSGGQRQKIALARALLTNSSILIMDEATSNIDILTERKIINNLMALNKTI
                     IFISHNLNITQKVDKIFVLAKGKIIEEGSHEFLLKAKGYYSNLINNGIFCINTTNVKNE
                     MKVLFFHTFYFLNFTIIT"
     gene            complement(6941..7138)
                     /locus_tag="EL085_RS12510"
     CDS             complement(6941..7138)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /locus_tag="EL085_RS12510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="WP_197737723.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.1e-11, bitscore:
                     39.7, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFLRRKLRTTVNGTTAYGLVQVADKLGFETVPIKADISLFEIDD
                     LQYPFIVHVKKITIIFTIML"
     gene            complement(7062..7253)
                     /locus_tag="EL085_RS12515"
                     /old_locus_tag="SAMEA3504576_02017"
     CDS             complement(7062..7253)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /locus_tag="EL085_RS12515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02017"
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="WP_197737724.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.6e-08, bitscore:
                     29.8, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFSKRFYRAQVDSQDCGAAALAMILEFYGSHYSFSLFFGFSEKK
                     VKNNSKWHYSIWFGSGSR"
     misc_feature    7432..7445
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: strong, score: 21.85"
                     /tool="antismash"
     misc_feature    7459..7474
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 24.05"
                     /tool="antismash"
     gene            complement(7628..7936)
                     /locus_tag="EL085_RS09805"
                     /old_locus_tag="SAMEA3504576_02018"
     CDS             complement(7628..7936)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024424.4"
                     /locus_tag="EL085_RS09805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02018"
                     /product="DUF3884 family protein"
                     /protein_id="WP_063284913.1"
                     /transl_table=11
                     /translation="MIKISLKKILCQLSQKKLYEKTFQSIYIVDFSLLDRAPLFKDEFK
                     VIGTWYSYSGKRWICHTELSTEQFKKMITKNIDHKDLEKVKFYLDYLPFSITNEIPF"
     gene            complement(8011..9141)
                     /locus_tag="EL085_RS09810"
                     /old_locus_tag="SAMEA3504576_02019"
     CDS             complement(8011..9141)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /locus_tag="EL085_RS09810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_02019"
                     /product="glycosyltransferase"
                     /protein_id="WP_159306489.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 5.4e-19,
                     bitscore: 65.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEKINKLEIISEYTTISVVYIVKNERDYIIKSLLSILDLADEII
                     IVDTGSEDNTLDLIKKMCDKKIKIFTFNWCDDFSKARNFANAKATCDWIMILDADEIVR
                     KNDNLKFYLTYLRMFDDFENTAFNFKVIRDEEVYKTSKLIRNTNTLTYKGRVHETFFSL
                     NNSVSYANLNVEVLGIRRGSQKKTNYYNKLLLKTIIDFPKEGRWYYLYLRDNIENMNYN
                     QMLKFCKKIIFKRDAISGIEINNNYYSVRIIFLLMFKLLEESNHERFTYYMELLEKDFN
                     HTQDFIFLKYSYYIKKLNIDATNLLKSFLENYDLLNEGDVIFSKECTHSLLGASLFWGG
                     YLKESKKVFEELEINSKDTVCFYNKKFIYDCLSTLS"
ORIGIN
        1 attttatcat acaatccctc tatctttttg ttttcaatta attttactga tatacaattc
       61 atttccccaa atggctagac acaatattca ttaaaacata attcattgta aaaataacac
      121 aatttgcatc taaaaaattt aaatcatcta ctatcgacac gtctcacttt tccattcttt
      181 accactcaat aagaaatcgt ttatttgtaa aaaaggagcc aggaattccc ccagctccat
      241 ctgatgtttt acgaacgatg cattttatat tcttgaatcg cttctaaaaa ttcaggacaa
      301 tagtgcttca gcttataact accaacacca tcaatctgaa tcatttcttg ctttgtgaca
      361 ggctttcttt tcgcaaaagc ttctaacgta tagtctgtaa aaatattcgt cggtggcatg
      421 tttaatcgtt cgctcatttc acgtctgact tcgacaagct tttcaaataa cgtgcggtcg
      481 accccttcta cagtgttaat atgcactgtt tctttcgact tacgcttgaa cggccttgta
      541 aacacttgcg tatcctcact caataacttt tctactgagg catcacacat gagtatttcg
      601 tcatgttcgt tgagaaagcc tttaaagcgg agttcatcga ttaaatgatg taactcgcca
      661 gtcgtataat ctttcatcaa accatacgtc gaaagatttt catagccttg atgacgaatg
      721 tagtccgaaa cctcgccacg caaaacttga atgaccgttt gatatccttc ttgttgacgt
      781 aaacgtgcga caccgctaat gatcattttc gcttctttcg tcatattgta cgttttattt
      841 ttatcaatac aattactaca ttggtgacat tcttcaagtt tttcattcgg ctcaaaataa
      901 tggactaatg ttgcttctag acatttcgtc gttttcgtat attgaatcat tttcgtcaat
      961 ttttcgccca tacgttcctt gtaatcatcg tccgctttcg aggcaccgat aaaatactga
     1021 tgtaaagcaa tatctcgatc actgtacaat aaaatacaat cactatctaa tccgtctcga
     1081 cctgcacgtc ctgcttcttg ataatacgat tccaaatcac ccggcatatt gtaatgaatg
     1141 acataacgga cattcgattt atcaattccc ataccgaatg cgttggtcgc aacgactaca
     1201 cgagtacggt catataaaaa gtcattctgg gcttcatctc tttgtttcgc tgtcattcct
     1261 gcatgatagt aggatacagg gatatctgcg tcttctaaag cttcatacaa ggcttctact
     1321 tgtttacgcg ttgaacaata aacaatcccc gctttctgac tatgtttttg aatataatcc
     1381 aatacaaact tttgacgttg atacgtccca ttcacctgga acttcaaatt acgtcgacga
     1441 atactagttt tgactaagtg ttgtcgcgaa atcattaacc gttccataat atcttgttgc
     1501 acttcagtcg tcgctgttgc agtcaatgcg acaatcgaaa actgttgcgg catagtcaat
     1561 acgcgttgta tgactgcttg ataactcggc cgaaaatcat gtccccattt tgaaatacag
     1621 tgtgcttcgt caaatgcgac gagtgaaata tcgacacgtc gcaacaacat ttcaaaataa
     1681 gtttgctcaa aacgttccgg tgcgatgtat aaaaatttca actcaccatt gaccaattgc
     1741 gtttctatct ctttttgtgc ttgcgtactt aaactactat tcaaataagc cgcagatatt
     1801 cccattgcgg tcaattggtc cacttggtct ttcattaacg aaattaaagg actaatgaca
     1861 atggtcgttc ctttaaacat caatcccggt acttgataac ataacgattt acccccacct
     1921 gtgggtaaca cccctaacgt atgatgatga ttcaatacgc gttcaataat ttctttttga
     1981 ccaggacgaa aagatttgta tccaaaataa tgcgacaatg tttgctccat gctatccctc
     2041 tattgttcta atatttcttc tagttcactc caccgcgtca tgtcggtatc atattgagct
     2101 tcgagcgttt ctttttcttc atttaatgct ttgattttgg cataatctgc gttcgcttcg
     2161 atcatttcgg cgtcaatttc agccagacgc gactctgttt cctcgaggcg cactaataac
     2221 atgtcatatt ctcgtttttc gttgtatgat aatccttttt tcttttttgg ttgtaatgtc
     2281 ggttgttgtt tcacggacgc tacttgagtt gctgcttgtt ccaattgttt ttggtatgct
     2341 aaataatctt caaactgccc taaaatcatg tcaatttgac cgtcgtgaat ataccaataa
     2401 caatccacga ctttatctaa aaagtaacgg tcatggctca cagtaatgac ggtaccacca
     2461 aatgtttgaa tgtagtcttc taaaatcgtt aacgtttctg tatccaaatc atttgttggt
     2521 tcgtctaaca acaacacgtt cggctgatgc actaacaact tcaataaata caaacgcttt
     2581 tgttcgccac ctgagagttt atacactttc ttaccatgcg tcacactggg gaataaaaag
     2641 cgttccaaca attgcgtaat cgaaacggtc gtcccatctt tttgacgcgc caactcactt
     2701 tcttcacgca agaaatcaat gacacggaca tcacgattca atcgttcatc tgtttgttta
     2761 aaatatgcca cttttaccgt ttgacctatc tttaatacac cttcaaaatg ctgatcttca
     2821 cctgctaaaa tgttcagcat cgtcgttttg ccgacaccgt ttgggcccac aataccgatt
     2881 tgaacacctt tttgaatcaa tgtcgacacg ttttcaaata atgtacggcc atcaatacgt
     2941 ttcgtcacat tttcaagttc aaagacttgt ttgcctaaac gcgaataagc cagattcaat
     3001 gaccctttcc cttgcacttg atgcgattgt acttgttctt tcaagtcatg aaaacgatct
     3061 tgccttgctt gttgcttcgt agttcgcgct ttgacaccgg cacgcatcca cgctaactct
     3121 tgtttaaaaa gtgcacgttg tttcgtttgt tgacgcactt cgatttcttc tcgctctgct
     3181 cgcattgcaa tatacgcttc atagttacct ggatatgtat ctagcttgcc atttcgcaat
     3241 tctacaatac gcgtcgccac ttcatttaaa aagtaacggt catgcgtgac aaaaacgact
     3301 gttttcggat aagactttac aaattgaatt aaccaatgaa tcgattcgaa atctaaatgg
     3361 ttcgtcggtt cgtctaataa taataaatct ggtttttcaa tcaacgtacg cgctaacact
     3421 acgcgctttt gttggccacc tgagagttca ctcattcggc gtgtcgtatc atggatgcca
     3481 agtttcgata aaatcgtttt aatctcagca ctataatccc aagcactgta agcatccata
     3541 tcagcttgtg ctgccatcat tgcatcaaac gctttgtcag tctgttgttc tgcgtattgt
     3601 gcagtagctt cttcataacg tttaatgact tgcaactctt tggaatctgc actataaacc
     3661 gcttcataaa ccgttaacgt tggctccaaa ttatgatttt gtgccgcata acgaatacga
     3721 tattgtttcg gatgcgattg aacggcatca aaatcttcat ccatgcctgc gatgactttg
     3781 agcaatgaac ttttccccgt tccattaata ccaactaatc cgattcgctc ttgattggaa
     3841 atggataact gtaaatcatc aaaaatgact ttatccgcat aagatttgtt gaggcgttca
     3901 attttatacg cttccacaac acatacaccc tttttcttat agatttactc gcatttatct
     3961 gttaacctta aatcagttct atatattata cacttttttt ggaacaaatg atacaaggag
     4021 ctatttctat gttggattat atttcaaaca tgccgccctt tttacaagca ttacttgcag
     4081 gtgtcatcac gtggttatta accgcattag gggctgcaac cgtatttgta tttaaaagtg
     4141 tcaacgaaaa agtgttgaat tcgatgcaag gctttgctgc tggtattatg atcgccgcaa
     4201 gtttttggtc attactccaa ccggccattg aaagcagttc gggcagtact gtaccgtggc
     4261 tccctgctgc aattggcttt ttactcggcg gggtattcat ccgttcttta gactatgtta
     4321 ttccacacat gcatcgaaac gcaactgatg aatcacaaca tcaagaaggt gttgagacga
     4381 gtctaaataa aaacacactt ttattacttg cgattacatt gcataacatt cctgaagggc
     4441 tcgcaatcgg ggttgccttt ggtggtattg cgacaggaaa tgaacacgca actttgttag
     4501 gggcactcgg acttgcaatt ggtattggga ttcaaaacat tcctgaaggg gctgcattat
     4561 cattaccgat acatgcttca ggacagtcca aatggaaagc cttcaactat ggacaagctt
     4621 cagcacttgt agaacctgtt tttgctacag tgggcgcagc tgctgtattg gtcgttactc
     4681 cgatactacc atacgcactc gcttttgctg caggggcgat gatttttgtc gtcgttgaag
     4741 aattaattcc tgattcacaa gccggtaaaa acacagatct tgcgacacta agcttgatgg
     4801 ttggctttac catcatgatg atactagatg ttgctttagg gtaaattcaa aaaaccgttc
     4861 aatgtgatga aacactgagc ggttttttga tgtgtacata tatcgataca ttaaaaatac
     4921 tctttaacaa ctcacaacaa agatttcaca cggttgacta tataaaattt caaccaatat
     4981 accaaaatat aataattaac ttaagttata attgtaaaat tcagaaaata aaaggtgtga
     5041 aaaaacagta ccttcatttc gttcttcaca ttggttgtgt taatacaaaa aataccatta
     5101 ttaattaagt tagaatagta accttttgct tttaataaaa attcatgtga gccctcttct
     5161 ataatttttc cttttgctaa aacaaatatt ttatctactt tttgagttat atttagatta
     5221 tgggagataa atataatagt tttatttaac gccatcaaat tattaataat ttttctctcc
     5281 gtgagtatat caatattact cgttgcttca tccattatca atatagaaga atttgttaat
     5341 agtgctcttg ctaaagctat cttctgccgc tgacctcctg acaaagcaga aaaatcagaa
     5401 cttaatacac tatcaagtcc aagtggtaag ctatcaataa aggattttag ttcagccatt
     5461 ttaatagcac tatctatgtc cttttgcgta acctctttaa ctacccctaa taaaagattt
     5521 tcaagtatag ttccattaaa aatataagtt tgttgtggaa tgtatataat ctgctgtcgt
     5581 aaaacagatt tattgatatt tgttatgtcc aatccaccta gtgttatttt accctttgaa
     5641 ggttcaaaaa aactaaccat caatttcgcc aaagttgttt tacctgatcc agactctcca
     5701 ataaaggcta ctttactatt atgttctatt gttatattaa tatctgtaag gatttctttt
     5761 tcgtacacat atccatatga aatatttgaa aatataagtt tacttgagtt ttggttcaat
     5821 ttgatatcca atttattctt ttcaaattct gatttaactg tgaatatttc attcaatcca
     5881 ttattagcca cacctgctat ttgtaattta agctgtaaat tcataatatt ttctattgga
     5941 ttcataaagt aaatcattaa tgaattaaaa acaagcaaag ttccaattga aatttgattg
     6001 tctataacta ggttagcgcc tacccataaa atcgatacat ttaatattaa ttgaacaacc
     6061 tttttcaaaa cgttttgctt tatatttagt ttgtttaaca agaatgtttt ttcgtaataa
     6121 ttcgaaaaat tatattttat ctgcttatag catttctttt cacatgtcag tgcttttatc
     6181 gtctccatac ctgttatatc ttccatcaaa taagaagaca aagtagcatt tgcctccata
     6241 gattttattc ttttccgttc aaaagagttc ataaaaccaa aaacaattac ggcataaata
     6301 ggaatgataa ttaatattaa tacaaataaa atagtacttt gataatacag gaagatagat
     6361 atgccaacta ttattgtaac atctaaaaac aatgatagca tggtactagc taaagcatca
     6421 gtaattgaat ttgtatcatt aagtcttgat agtatgtctc ctgctcttcc cgtttcaaaa
     6481 aaagtaatag gtaatctaaa caaatgttta atgtatggtg aaagtatatt tatagaatat
     6541 gtctttacta ataaaatcaa aaaatatttc tgcaaataac taagaatttg ttgcataatg
     6601 taagaagcaa ttaaagccat ggaaatcata ctaacccatg tttttaactt ttggggaata
     6661 taaaaatcaa taattgactt cataaaatat gaacctaaaa tatttatcat attgactaaa
     6721 atggaaatga ttattatttt tataatcaat cttttttctt ttaataataa aggtataaaa
     6781 gaagttaaac gaactttatc ttgctttata ggcttatata aagatgtagg ttttaataag
     6841 attgtaactc cagtccactc ttctttaaat tccctataag tcatctttgt taaattaaca
     6901 acagggtcag ctatatagat atacttttta tcacatctaa ttacaacata atagtgaaaa
     6961 tgattgttat ttttttaaca tgaacgataa aaggatactg taaatcatca atttcaaaca
     7021 aagatatatc tgctttaata ggaactgtct caaaacctaa cttatctgct acctgaacca
     7081 aaccatatgc tgtagtgcca tttactgttg ttcttaactt tcttctcaga aaatccaaag
     7141 aatagtgaga aagaatagtg agaaccgtaa aattctaata tcatagcgag cgctgccgcg
     7201 ccacaatctt gagaatctac ttgtgcacga taaaatcgtt ttgaaaattt cattcgacac
     7261 ttctactcct tcccaccact ttttagaaca acatttaaat attcaaaaag cgggcggtaa
     7321 ttcccaccca cttaatatca tgtcaaaatt caaacctatt attaaaagct ttaatggtta
     7381 ttcatcttta catatttttt tcatataatc acatgcactc tctggatcag atgtaccgaa
     7441 tctaaccaat atactgtcgt ttatacatgt aatctgagcc catttacaac gggacatccc
     7501 accacttaag tctttcagca cctgatttga tatcacttta aatttatcaa tactttttat
     7561 atctatccct cctaaataga acaatttaat aatactatta aaaatcaatt aaatgtatga
     7621 tttattttca aaacggaatc tcgttagtta tagaaaatgg gagataatcc aaataaaatt
     7681 tgactttttc taaatcttta tggtctatat tttttgttat cattttcttg aattgctcgg
     7741 ttgataattc cgtatggcat atccaacgct taccactata gctataccaa gttcctatta
     7801 ctttaaattc gtctttaaat agaggagccc tatctaaaag actaaaatca actatataaa
     7861 tactttgaaa tgttttttca taaagttttt tttgagataa ttgacataaa attttcttta
     7921 aagatatttt aatcatagat ttctcactcc tttacactac ctgttatctt aatgcggtag
     7981 attcctgagc tataataata tcaatattta ttagcttaaa gtcgatagac aatcataaat
     8041 aaatttttta ttataaaaac aaactgtatc tttactattt atttctaatt cttcaaatac
     8101 ttttttactt tcctttaaat atcctcccca aaataaagag gctcctaata acgaatgagt
     8161 gcattctttc gaaaaaatta catctccctc atttaacaaa tcataattct ctaagaaact
     8221 tttcaataaa tttgtagcat ctatattaag tttctttata tagtatgaat atttcaaaaa
     8281 aataaagtct tgagtatgat taaaatcttt ctctaacagc tccatatagt aagtaaaacg
     8341 ttcatgattg gattcttcta ataacttaaa catcaaaaga aaaataattc taacagagta
     8401 ataattgttg tttatttcga ttccagatat cgcatctctt ttaaatataa tttttttaca
     8461 aaattttaac atttgattat aattcatatt ttctatatta tctctcaaat ataaataata
     8521 ccaacgacct tcttttggga aatctataat tgttttaaga agcaatttat tataataatt
     8581 tgttttcttt tgacttcctc ttctaattcc taaaacttct acatttaaat tagcataaga
     8641 tacagaatta tttaaagaaa agaatgtttc gtgtactcta cctttgtacg tcaaagtatt
     8701 agtatttctt atcaacttac ttgttttata tacttcttca tctctaatta ctttaaaatt
     8761 aaaggccgta ttttcaaagt cgtcgaacat tcttaaatac gttaagtaaa actttaaatt
     8821 atcatttttt ctaacaattt cgtcagcatc taaaatcata atccagtcac aagttgcttt
     8881 agcatttgca aagtttcttg cttttgaaaa atcatcacac caattaaatg taaatatttt
     8941 tattttttta tcacacattt ttttaattaa atctaaagtg ttgtcttcag aaccagtgtc
     9001 aacaataatg atttcatcgg ctaaatctaa aatactcaaa aggcttttta taatataatc
     9061 tctttcgttt ttaacaatat acactacaga aattgtagta tattcactta taatctctaa
     9121 tttatttatt ttttcaatca attctgtatc gtttttaact cttttagact cccaatcttt
     9181 tgattctata aatttatttt gttgtatgtt tcacaagaaa tattaagaaa tgccttattt
     9241 ttttctaacc aatcttcatc aaatataggg tatatgtttt tcaaagttaa tcacaccgcc
     9301 tctaaaaaaa taataaaaca aaatattttt aaaagttttg caagactggg aggcgaatta
     9361 ttatgataaa tacgtaattc tactatcaca tttacaaatt actatgcttt aattataaat
     9421 aattgtatta ctttgtaaat atagtgtcaa tatttattta tattcggaag tataatttct
     9481 cacatatact ttaattacag aagtacatgc agtgtagatg acggttataa aatgtaggaa
     9541 aatggcgaaa tgaaaatgct gcatttcata tataaacgcc aagataaata tggacaagtt
     9601 tattcttttt atgaaaggaa atgttttaat ctaaaatact ttccaacgat agaggcgatt
     9661 gagacgggtt ctttgtcaac gtcggttggt gagacaataa cggcactgag acattatggt
     9721 ttcctaatct ctgcttctat ttatagactg atatcgatta ataatgattg atccttcgaa
     9781 aataaggggt gaatgtgtat aaaaaatgat gccattgttt tctggatttc ataatcttgc
     9841 actcagtttc gctgtattga tataggatta cccagatggc tgagacgcct gagggggaac
     9901 gtgcatactg ttgacagtcg cctctttact ttaatggtgg gaggcgttta tcgcagtagc
     9961 gggctgactt ctcagtgttt tcacttttga gaagtctaat cagccatgca aggcagtaca
    10021 acgaaatctt gttgcatatg aggtttctgt actgctccta aaatccaatt tggcttcaat
    10081 caagccgaat tgagttgaag ggccggctct aggaaagcga aaccatgatg acaattaaaa
    10141 ccacaaatcg taatgcggac aagtttaata aaaatattgc aacaatagca tcaaaaattt
    10201 ttaagaccca ttccgttttt tatatgcact tacaagtatc ttagaaatga ttaagattcc
    10261 agaaatttct ataactataa gacacacatt tgttttatta ttaatccttt caatcgggcc
    10321 atttgttaaa tgaggatgat gcatagtatt tgaggttcag ggcaaatatt tgatgaatgt
    10381 ttgcaacgat taggtaaaat gtcgatgata tcatgggtta cactatcaca gtaaataaag
    10441 ctcattggtc cttcgatatc tttcgttgat ttgaattcgt caaaagctaa gtgctcaggt
    10501 aaagcatcat gtgcgtatgt ctttacaaat tactcatctc ttttaaatgg caatgaacag
    10561 ttgaaggtga tacacataga cttttagcga catctttttc agatgtgaat ttagttagct
    10621 tccgcgtcat caagcgtttg accttatttg agacttcggg gttgaatata gggtgtttta
    10681 gttataaagg tttgttgaca agctttaaag taaaaacgat gcttcttcaa atttaaataa
    10741 acaggtcttt caaaatttaa aaacgggcag taaatctctc accaaccgtt aaaaatacac
    10801 aaccaaatat atacaaccta aaaaacaggg aaatgcacta atttgtacac ttccctgtca
    10861 taacatttta tttttgattt gcctttggac ctgtcggata tttataatct gctgggttca
    10921 atttgttgaa atcaggattt ttatagaatc ggaataagtc tccattcaag actttatcac
    10981 tcatttctaa gtcatgttgt acctgctttt tgtccttatc aagatctgct ggcggtgttt
    11041 ctaacggttg attgtctctg ttactgtagg ctttaccgtt gacatattta taatttctcg
    11101 tcacgaaatc accattacgg aatggtacaa tttctttatg gtccttggat aacatatctg
    11161 taccaaacat gagataattt ttcgtatcaa tacctaacaa gtgaagtaat gttggcatca
    11221 catcaacttg acctgcatac gtgtcgtcca ctttaggctc aacccctggt gcttttaacc
    11281 agaaacccgt tgcattcaag tcattaaact tcgctggtgt aatctcttca cctaataact
    11341 gtgacatcgc tttgttatga ttttctgaga taccataatg gtcaccgtaa atcataatga
    11401 ctgaatcatc gtacagacca cgctctttca agtcattaat gaactgttct aacgactcat
    11461 ctaagtaacg cgctgtttga atgtaaccat cgaccgttga gttacctgtg tcaccttttg
    11521 caatcgttgc gtcttcatca cttaatgtaa aaggataatg gttcgtcaac gtaattaaat
    11581 gtgaatagaa cggttgtttt tctttagata agtaatccgc cgcctctttg aaaaactctt
    11641 tatcttttaa tccaaggttt tctaagtttt ctggagacat gtcaaaatat gttgcatcat
    11701 aaaacttatc gataccaaaa tgtctgtata cttggtcacg gttccaaaat gttttatagt
    11761 caccgtgcat gacatttgtt gtatagcctt gttgttgatg cataatagct ggtaaagatt
    11821 gaaaagtatt gtcacctttt aatgaaaagg ccgaaccttg aggtaaacca tacatactgt
    11881 tgtccattgt gaactcagcg tctgacgttt taccttgtcc agtttggtga tagaaatgtg
    11941 ggtaatatct aaagtcctca tt
//
