LOCUS       NZ_LR134267            11044 bp    DNA     linear   CON 15-APR-2024
DEFINITION  Staphylococcus pseudintermedius strain NCTC5661 chromosome 1.
ACCESSION   NZ_LR134267
VERSION     NZ_LR134267.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus pseudintermedius
  ORGANISM  Staphylococcus pseudintermedius
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus; Staphylococcus intermedius group.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2019) WELLCOME SANGER INSTITUTE, Pathogen
            Informatics, Wellcome Sanger Institute, CB10 1SA, United Kingdom
REFERENCE   2
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2018) WTSI, Pathogen Informatics, Wellcome Trust
            Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900636355.2-RS_2024_04_15
            Annotation Date                   :: 04/15/2024 03:28:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,432
            CDSs (total)                      :: 2,350
            Genes (coding)                    :: 2,313
            CDSs (with protein)               :: 2,313
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 12 of 37
            Pseudo Genes (incomplete)         :: 27 of 37
            Pseudo Genes (internal stop)      :: 6 of 37
            Pseudo Genes (multiple problems)  :: 8 of 37
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:45:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 908881
            Orig. end                         :: 919925
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR134267.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11044
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11044
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11044
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6044](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6044
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(461..479)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 24.84"
                     /tool="antismash"
     gene            1028..2389
                     /gene="rlmD"
                     /locus_tag="EL085_RS04005"
                     /old_locus_tag="SAMEA3504576_00819"
     CDS             1028..2389
                     /EC_number="2.1.1.190"
                     /GO_function="GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="rlmD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147863.1"
                     /locus_tag="EL085_RS04005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_00819"
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="WP_126510896.1"
                     /transl_table=11
                     /translation="MVLVKKNEVYEGKVVDLTHEGHGVIKQDRYPIFIPQTLIDEEVQY
                     KVIKVKKNFAIGKLMNVKTASPDRVTPPCDYYQQCGGCQLQHLSYRAQLEMKRKQVINL
                     FHHKGKMTEVPIHDTIGMDDPWRYRNKSQIPVGTNRNGQIQMGFYRQRSHDIIDMDHCL
                     IQNDDQNDMMNVIKSLLEKYHIPAYNEHIHQGLVRHVVIRKGYYTNEVMVIFVLNGKKL
                     PHHNDIVTALTSQFPQIVSIKLNFQTNKTNVIMGHTSKTIFGKDIIEDTLDDLTFEIGD
                     LSFYQINVTQTQKLYRQALQYANLTGNEVVLDAYCGIGTISLFMAPHAQHVYGVEVVPE
                     AIEDAKRNARYNQLNNTTFVAGAAEDVILKWQQDGIQPDVVTVDPPRKGCDATFIETLN
                     ALAPKRIVYVSCNPSTQLRDIEMLSTQYELKEMTPVDMFPHTTHVETVALLVRK"
     gene            2515..3834
                     /locus_tag="EL085_RS04010"
                     /old_locus_tag="SAMEA3504576_00820"
     CDS             2515..3834
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1206: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015729285.1"
                     /locus_tag="EL085_RS04010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_00820"
                     /product="ISL3 family transposase"
                     /protein_id="WP_126510897.1"
                     /transl_table=11
                     /translation="MCNDISEILGIKVKNLKITQNLGLHVHKNVQALFYEGQLTYHPSA
                     CECCGIKNDNHLIIKHGFRKTNVYMGLIFERPAYLKLKKQRFYCKACQRTFTAETPYIQ
                     PRCTISNEVKRMMTWKLSKVTSEKDIAENLCVSPSTVHRHLKVVSNSVKTHAHYVLPEH
                     LAFDEFKSTKDVEGAMSFIYCDSVTHDIIDILPDRRKHQLEAYFLKFSRKQREKVKTVS
                     IDMFPPYIALIQDLFPHAEIIIDRFHIVQAINREINRCRVQVMNGFRTKERPQYNKLKR
                     YWKLLLKAPIDLDRMIYQPYRLFKSWQSQYSLVQYLLNLDETLKETYETGHRLLSALKA
                     NDIQQLRFILQDAKIKDTSKGLKRVIHTFIKYMPYISSTMRHRHLTNGPIEGINNKIKL
                     IKRVSYGYRNFWNFRNRILMISKVFVSEYKKRIKQQNNVA"
     misc_feature    4034..4052
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.21"
                     /tool="antismash"
     misc_feature    4114..4128
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 26.38"
                     /tool="antismash"
     misc_feature    complement(4139..4154)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 23.45"
                     /tool="antismash"
     gene            4199..4948
                     /locus_tag="EL085_RS04015"
                     /old_locus_tag="SAMEA3504576_00821"
     CDS             4199..4948
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EL085_RS04015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMEA3504576_00821"
                     /product="hypothetical protein"
                     /protein_id="WP_037542633.1"
                     /transl_table=11
                     /translation="MGGINCGLSNNRVVKNDMYRLEYALYTKYVFKCFENFCNANTWVE
                     EFTLLEIDDNDNALVAYKKALFAIQEAITNLQSAKNALINSYNEIAPLFKFSQLKNGSI
                     NQYKKYDHSFAKGVFEKAGVVTNETKVWESVIHSLQNGGEVDYLNDQQNKLIVFENTLQ
                     NLFEEYQKLEKYIRQGVSHVAIRDIEVDITPLTAMALTKISELMSSLTYLCLVEYSAHT
                     SITGKTIDVLDLLPKTNKNSIQHLKAN"
     gene            5001..6044
                     /locus_tag="EL085_RS04020"
                     /old_locus_tag="SAMEA3504576_00822"
     CDS             5001..6044
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="EL085_RS04020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_00822"
                     /product="YcaO-like family protein"
                     /protein_id="WP_014613464.1"
                     /sec_met_domain="YcaO (E-value: 2.4e-22, bitscore: 77.2,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLENVSEHGVSGFYTYHFTEGNSFRPCATRIIEKEAIKATKDEFM
                     ELMLISSKSFPFVFFGGKSLGNFKLPIHLHPFMWQKRTIQDVNWYNEKNVEYIPFYNYK
                     DGSLKYIPKELSVYYEKAKGIGLSFSPQEGYSYDRDIVLAKVKAVMELIEKDIVTLWWH
                     RETSSRIIVIDDDTDENIKIIRNQLLNQGIDITLLDIENDLGVYVVVCILKQDDFPSIT
                     YGSAAHFEIKGAVKHAIFEAISCIVGLRYEFIHFKKIRTSFTYPDFISDSPLTNLSDYK
                     EILNLKTAINKYDIYYSYISADEGYLVKAYCYELQPTLYSATVPLTKRFFRVNKKEVRI
                     KEDFPFI"
     gene            6055..7245
                     /locus_tag="EL085_RS04025"
                     /old_locus_tag="SAMEA3504576_00823"
     CDS             6055..7245
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="EL085_RS04025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_00823"
                     /product="MFS transporter"
                     /protein_id="WP_014613465.1"
                     /transl_table=11
                     /translation="MKTKRIIASDFLTTMMSSALTIYIYWFAYQYFSSQLIISIIGFGQ
                     LSGVFLSSLGGGISDSMNKLFFIKTLNFFKVGLLFMVLILENVVNMEILLPLFMFASTV
                     IGGLQSPTLESLVPFLASSDEALFRINAVVSSSTQVASIMAIVLSAVYISFLSFVTIVF
                     VTLFITAIATILLVGLTVETPLKSTSVVNNMKHGASYIFKTPYIRNLIPVALVMNFSFW
                     SIFLLLPKIANDSFSFLKTSYSGMELSFALGAGLGGILFAKFFYDVKDKYRLFKRSLFS
                     QSYVLLLLGIVLLIPNSVLAYTGMLLCWFLYALINTIFSILYFGKIQLNVPREMIGSVF
                     GAILTLFSLVNPIAAIMSGVLVSHFKIPLLIILLSSLMLLAAWSIRFLADVETVFD"
     gene            7259..8626
                     /locus_tag="EL085_RS04030"
                     /old_locus_tag="SAMEA3504576_00824"
     CDS             7259..8626
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="EL085_RS04030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_00824"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_037542634.1"
                     /transl_table=11
                     /translation="MSILTLKNIRCEMDGKLLFEADGLTIEEGDVIGLIGKNGAGKTCL
                     LKILAGELSDYAGYISGNRLTYFSELCITEDMTQSGGELSISSFLNALRQSVPLYLLDE
                     PTTYLDQHHLKKVVRTIRRSPSSFCIASHDRAFLDAVATKIWLLEQGKITEYNGSFSDY
                     VLARSMQLSQYEQELKQYQKEKKKIKQSLRMMKEKQDQKKGKPKKMSTSEYRIAGIKTK
                     YGVKQKKLQKRITRQQKKLEQLKQPYQVEDKYDISFLSQLHCLPKRKIIIPSHEAKIGQ
                     KSLWHTPTIHLNSGDKLGIIGRNGAGKTTYLNYLISVLPSTYKICYFRQNHFQYLDPKM
                     TVYEAIVEATNGYLSEHHIRTLLALLDFKRDKVFRLIGDLSRGEHVKISLLSLLVSESD
                     ILILDEMTNFLDIKAIEAVEKVLATYSGILIFVSHDQYFVQRLATSILDIDTYKIM"
     misc_feature    8818..8832
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 24.36"
                     /tool="antismash"
     misc_feature    complement(9006..9020)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.13"
                     /tool="antismash"
     gene            <9159..9389
                     /locus_tag="EL085_RS04035"
                     /pseudo=""
     CDS             <9159..9389
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014613462.1"
                     /locus_tag="EL085_RS04035"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /pseudo=""
                     /transl_table=11
                     /translation="DVVTVDPPRKGCDATFIETLNALAPKRIVYVSCNPSTQLRDIEML
                     SAQYELKEMTPVDMFPHTTHVETVALLVRKY"
     gene            9863..10747
                     /locus_tag="EL085_RS04040"
                     /old_locus_tag="SAMEA3504576_00825"
     CDS             9863..10747
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR01716"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR01716.1"
                     /locus_tag="EL085_RS04040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA3504576_00825"
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="WP_065354136.1"
                     /sec_met_domain="TIGR01716 (E-value: 7.7e-41, bitscore:
                     137.2, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNYGKTFRKIRKSRGIKLKDIAKMGISVSQLSRFENGETNLTIS
                     KFILILNEIMMPLDEFMNEAHDFRNNRLNERFEHMIYFTIENDILRMKQLLFDEMKKNT
                     QRSHFYYLNLILIKIRLQVMTGELYYTKDDLEVLIDYLFTTEYWGYYELYLFTNTLDVI
                     NHETMMLLSREIVSQTKALNKLQLYRRMLSAMLLNGYMTCIQRQKMKDAIFFEKCIERC
                     LFTETEIYEKLVFQYAKHFLIYSKSSSEDAINKMKKIIEVMRLVESHHIASYYENHLNH
                     ILCEKNVHIWDSL"
ORIGIN
        1 aattaaaatg gaacaagctg gttatgagac gagtgcctat gcgactcaaa aggttggcga
       61 tgcgacagaa gaggcggcgc gtgcgattga ggcccaatac gatttgttaa ttgtcgctgg
      121 tggagacggt acattgaatg aagtcgtgaa tggtattgct gaaaaaccga atcgtcctaa
      181 gttaggttta attccaatgg ggacggtgaa tgatttcggt cgagcactac atctcccaac
      241 tgatattttt gaagccgtgg atgtcatttt agatggtaaa actgttcaag ttgatattgg
      301 taaaatgaat agccgttatt ttattaattt ggctggcggt ggtaaaatta cggaagtttc
      361 gtatgaagca ccgagtaaat taaaatcgat agtcggacct tttgcgtact atattaaagg
      421 atttgaaatg ttacctcaaa tgcatgcagt cgatgtgcgg attgagtttg attcgcaagt
      481 ctttgaaggt gaaatcatgt tgttcttact cggcttaacc aattcaatgg ctggttttga
      541 gaagttagta ccagatgcga agttggatga cggtatgttt acactgttga ttgttgaaaa
      601 ggcaaatatt gcagaattgg gacatattat gacacttgcc tcacgcggtg aacatattaa
      661 acatccgaaa gtacattacc ataaagcgca atcggtaaat atttcttcct ttagcgatat
      721 gccgttaaac gtagatggtg aatacggggg tcaattacca gcgaactttt taaatttagt
      781 tcgtcatatt gaagtttttt ctccggcgca agaagataat gcgttgttaa ttgatgagcc
      841 tacgcaatct gagtaacgat ccagaggtgt gctgaaaata aataggtagt gtacgcaaaa
      901 ccgcatgatg taatgacatt actctcgtgg gagcgggacg atgaaatttg actcagatgt
      961 taagtttttg gtcctgctcc tttccctttg aagtagattc tgtttataaa aaagtgagat
     1021 gaaattgatg gttttagtaa agaaaaatga agtgtatgag ggaaaagtgg ttgatttgac
     1081 acatgaaggg catggtgtga ttaaacaaga tcgctatccg atatttatcc ctcaaacgtt
     1141 aattgatgaa gaagtgcaat ataaagtgat taaagtgaaa aagaatttcg cgattggcaa
     1201 gctgatgaat gtgaaaacag caagtccaga tcgtgttaca ccgccatgtg attattacca
     1261 acagtgtggg ggatgccaac ttcaacattt gtcgtatcgt gcgcaattag agatgaagcg
     1321 taaacaagtg attaatttgt ttcatcataa aggaaaaatg acagaggtgc cgattcatga
     1381 cacgattgga atggatgatc cttggcgtta tcgaaacaag tctcaaattc cggttgggac
     1441 gaatcgcaat ggtcagatcc aaatgggttt ttatcgtcaa cgcagccatg atattatcga
     1501 tatggaccat tgtctcatac aaaatgatga tcaaaacgac atgatgaatg tgattaaaag
     1561 tttactggaa aaatatcaca ttccagctta taacgaacac atacatcaag gtctcgtacg
     1621 tcatgtcgtg attcgtaaag gctattatac gaatgaagtg atggttattt ttgtgctcaa
     1681 cggaaaaaag ttaccacatc ataacgatat tgtgaccgct ttaacatcac agtttccgca
     1741 aattgtgagt atcaagttga atttccaaac gaacaaaacg aatgtcatta tggggcatac
     1801 gtccaaaacg attttcggta aagacattat tgaggataca ttagatgatt tgactttcga
     1861 aattggcgat ttgtcctttt atcaaatcaa tgtgacacaa acgcaaaagt tgtatcgtca
     1921 agcgttacag tatgcgaatt taacgggtaa tgaagtcgtg ttagatgcgt attgtggtat
     1981 tgggacgatc agtttattta tggcacccca cgcccaacat gtatatggtg tagaagttgt
     2041 ccctgaagcg atagaagatg cgaaaagaaa tgcgcgctac aatcaattga acaatacaac
     2101 ttttgtcgct ggtgctgctg aagacgtgat tttaaaatgg caacaagacg ggattcagcc
     2161 tgatgtggtg acagttgacc caccgcgcaa agggtgtgac gcgacattta ttgaaacttt
     2221 aaacgcgtta gccccaaaac gtatcgtcta tgtgtcatgc aatccaagta cacaactacg
     2281 agatattgaa atgctaagca cgcaatatga actaaaagaa atgacacctg tcgatatgtt
     2341 tccgcatacg acacatgtcg agacagttgc gttattagta agaaaatagg ttatatattt
     2401 ttaacggttg gtgagagatt cactgaccgt tattttaatt tttgatatta aaaaagcaca
     2461 aatatctcta taattgtaag tgaccaaacc aacgattaag gagatattgt gcctatgtgt
     2521 aatgatatat cagaaatact aggaattaaa gttaaaaatt taaaaatcac tcaaaattta
     2581 ggattacatg ttcataaaaa cgtacaggca ttattctatg aaggtcaatt gacttatcat
     2641 cccagtgctt gtgagtgttg tggaattaaa aatgataacc atttaattat taaacatggc
     2701 tttcgtaaaa cgaatgttta tatggggtta atttttgaaa gacctgccta tttaaaattg
     2761 aagaaacagc gcttttactg taaagcttgc caacgaacct ttacagctga aacgccctat
     2821 attcaacccc gatgtaccat ctcaaatgag gtcaaacgca tgatgacctg gaaactatca
     2881 aaagttacat ctgaaaaaga tatcgctgaa aatctatgtg tatcgccttc aactgtccat
     2941 cgccatttaa aagtagtaag taattcggtg aagacacacg cacattacgt tttacctgag
     3001 cacttagctt ttgacgaatt caagtcgacg aaagatgtcg aaggtgcaat gagctttatt
     3061 tactgtgata gtgtgactca tgatatcatt gatattttac ctgatcgtcg caaacatcaa
     3121 ttagaagctt attttttaaa gttctctaga aaacaacgtg aaaaagtaaa aacggtctct
     3181 atagatatgt ttccacctta tatcgctctg attcaggatt tatttcctca tgcggaaatc
     3241 attatcgatc gctttcatat cgtacaagcc atcaatcgtg aaatcaatcg ctgccgtgtt
     3301 caagtcatga atggttttag gacaaaagag cgacctcaat ataataaatt aaagcgttat
     3361 tggaagttac tattaaaagc gcccattgat ttagatcgaa tgatatatca gccatacaga
     3421 ctttttaagt catggcagag ccagtacagt ttagttcaat acctgttaaa tcttgatgag
     3481 acgctcaaag agacatatga aacaggacac cgtcttttaa gtgctttgaa agcaaacgat
     3541 atccagcaac tacggttcat cttacaggat gcaaagataa aagatacttc gaaagggctc
     3601 aagcgtgtca tccacacatt catcaaatat atgccttata tctcaagtac tatgcgtcat
     3661 cgacatttaa cgaatggacc cattgaaggg attaataata aaataaaact gattaaacgt
     3721 gtttcttatg gttatagaaa tttctggaat tttaggaatc gtatcttaat gatttctaag
     3781 gtatttgtaa gtgaatataa aaaacgcatt aaacaacaaa ataacgttgc ttaatgcgtt
     3841 atcgtctcac caaccgacgt tgacaaagaa ccagaaaata ttgatataga ttaatcactc
     3901 aagggttttt tgagaacatt tttgagaatg tgacttagaa cccttttttg ttttggtgct
     3961 attttaggag cgttgatact ggaactgatt tgacaaatag gtttgtcgtt aatagacatt
     4021 agctttttta aagtattgtt tagaaacaca gttgtgaaat tgtaaaaata ttgagattag
     4081 acattttgtg tttattaata tctttctaga attaatattc agaaaatttg gaaaaatagt
     4141 ttacatttgt gtatatttat gctacattca tgatggaata ttaagaggag gtgccattat
     4201 gggtggtatt aattgtggac tttccaataa tagagtcgta aagaatgata tgtatcgatt
     4261 agaatatgct ttatacacaa aatatgtgtt taaatgtttt gaaaattttt gtaatgcaaa
     4321 tacatgggtt gaagagttta cacttttgga aattgatgat aatgataacg cactagttgc
     4381 ttataaaaaa gcattatttg ccattcaaga ggcgattact aatttacaga gtgctaaaaa
     4441 tgcactaatc aatagttaca atgagattgc gcctttattt aaatttagtc aattaaaaaa
     4501 tggttcaata aaccaatata aaaagtatga ccattccttt gctaaagggg tatttgaaaa
     4561 ggcaggagtt gtgacgaacg agacaaaggt ttgggagtca gttattcatt ctctgcaaaa
     4621 tggaggagaa gtagattatt taaatgatca acaaaataag ttgattgttt ttgaaaatac
     4681 tttgcaaaat ctatttgagg aatatcaaaa attagaaaag tatatacgac aaggtgtatc
     4741 acatgttgca ataagagata ttgaagttga tattacgccg ttaactgcaa tggccttgac
     4801 taaaatcagt gaattgatga gttcattaac atacttatgt ttagttgaat attcagccca
     4861 tacaagtatc actggcaaaa cgattgatgt attggattta ctaccaaaaa caaacaaaaa
     4921 tagtatacag catctaaaag caaattgatt tttatatact gtgtctatac ggtacagttt
     4981 ttttatgtag ggggattaat atgttagaga atgtttctga acatggggtt tcaggatttt
     5041 acacatatca ctttacagag ggaaactcat ttagaccatg tgctacaaga attattgaaa
     5101 aggaagctat aaaagctact aaagatgagt ttatggagtt aatgcttatt tcaagtaaat
     5161 ctttcccgtt tgtatttttc ggagggaaat ccttaggtaa ttttaaacta cccattcatc
     5221 ttcatccgtt tatgtggcaa aaacgaacta tccaagatgt gaattggtat aatgaaaaga
     5281 atgttgagta tatccctttt tataactaca aagacggatc tttgaagtat ataccgaaag
     5341 aattaagcgt ctattatgaa aaagcgaaag ggattgggct atcttttagt cctcaagagg
     5401 gttattccta tgatcgagat attgtacttg ctaaagtcaa ggcggtcatg gaattaatag
     5461 aaaaagatat tgttactttg tggtggcata gagaaacatc aagtagaatt attgtgattg
     5521 atgatgatac tgatgagaat ataaaaatta ttagaaatca attattaaat caaggtatag
     5581 atatcacgtt attagatatt gaaaatgatt taggtgttta tgtggttgtg tgtatcctca
     5641 agcaagatga ctttccatca atcacttatg gtagtgcagc gcattttgaa attaaaggtg
     5701 cagttaaaca tgcaattttt gaagcgattt catgtattgt gggtttgagg tatgagttca
     5761 ttcattttaa aaaaatccgt actagtttta cgtatcctga ttttattagt gatagtccat
     5821 taactaattt atccgattat aaagagatat tgaacttaaa aacagccatt aataaatatg
     5881 atatatatta ttcttatatt tctgctgatg aaggatactt agtgaaagcg tactgttatg
     5941 aattacaacc caccttgtat agtgcaacgg ttcctttaac taaacgtttc tttagagtaa
     6001 acaagaagga agtcaggatt aaagaagatt ttccgttcat atagaaggtg agttatgaaa
     6061 actaaaagaa ttattgcgtc agatttttta acaacgatga tgtcttcagc cttaacgatt
     6121 tatatatatt ggtttgctta tcaatacttc tctagtcaac tcatcatatc aatcatcggt
     6181 tttgggcaat taagtggtgt gtttctctca agtcttggtg gtggcatctc agacagtatg
     6241 aacaagctgt ttttcatcaa aactctgaac ttctttaaag tcggattact ttttatggtg
     6301 ttgatattag agaacgttgt gaacatggaa attttgttgc cactttttat gtttgcttca
     6361 acagtgatag gaggtttaca gtcacctacg ttagaatcat tggttccatt tttagccagt
     6421 agcgatgagg cgctttttcg tattaacgct gtggtttcga gttcaacaca ggttgcatct
     6481 attatggcga ttgttttatc tgcagtctat atttcatttc tgtcttttgt aacgattgtt
     6541 tttgtgaccc tttttatcac tgccatcgca acgattttac ttgtaggact gacggtggaa
     6601 acgccattga agtctacttc agttgttaat aatatgaagc atggggcgag ttatatcttt
     6661 aaaacgcctt atattcgtaa ccttattcct gtcgcattgg tcatgaattt ctcgttttgg
     6721 agtattttct tgctcttacc taaaatcgct aatgatagtt tttcattttt aaaaacctct
     6781 tatagtggca tggagctctc ctttgcttta ggagcgggct taggcgggat cttatttgcg
     6841 aaattcttct atgatgttaa agataaatat cgtttattta agaggagttt gttttcacag
     6901 agttatgttt tgttgttatt aggaatcgtt ttacttattc ctaacagtgt tcttgcctat
     6961 accgggatgc tgttgtgctg gtttttgtat gctttgatca acacgatttt ctctattctc
     7021 tattttggaa agattcaatt gaatgtccct agagaaatga ttggcagtgt ttttggagca
     7081 attttaacgc tcttttcatt agtcaatccc atcgcagcca tcatgtcggg agttctcgtt
     7141 tcgcatttta aaatacctct gttaattata ttgttgtctt cactcatgtt gttagctgcc
     7201 tggagtattc gtttcctagc agatgttgaa acggtatttg attaaagggg ggagggatat
     7261 gtctatatta acacttaaaa acatcaggtg tgagatggat gggaaattat tatttgaagc
     7321 ggatggttta acgattgagg aaggcgatgt cattggcctt atcggaaaga acggtgctgg
     7381 aaagacgtgt ttgttaaaaa tattagcagg agagttaagc gattatgctg gctatatatc
     7441 gggcaatcgt ctgacttatt ttagtgaact ctgtataacg gaggatatga ctcaaagtgg
     7501 tggagaacta agcatttcaa gttttctcaa cgcattaaga cagtcggtgc ccctctattt
     7561 gttggacgaa cctacaacgt atttagatca acatcatttg aagaaagtag taagaacgat
     7621 tagacgctcc ccatcttctt tttgtatagc gagccatgat cgggcttttt tagatgcagt
     7681 tgcgactaaa atatggttgt tagagcaagg aaaaataaca gaatataatg gttctttttc
     7741 agattatgtg ttagcacgtt caatgcagtt atcacaatat gagcaagagt tgaaacaata
     7801 tcagaaggaa aaaaagaaaa taaaacaatc acttcgaatg atgaaagaaa agcaagatca
     7861 gaaaaaggga aaaccaaaga agatgagtac ttcagaatat cgtattgcgg gtatcaaaac
     7921 taagtacggt gtgaaacaaa aaaagttaca gaagcgtatt acacgacaac aaaaaaagtt
     7981 agaacagtta aaacaaccat atcaagttga ggacaaatat gatatttcat ttttatcaca
     8041 acttcattgt ttacccaaaa gaaaaattat cattcctagt cacgaagcga aaatcgggca
     8101 aaaaagcttg tggcataccc caactatcca tttaaatagt ggtgataaat taggaattat
     8161 tggaagaaat ggtgcaggca aaacaactta tttgaattac ttaatttcag ttttaccttc
     8221 tacatataaa atttgttatt ttcgccaaaa tcattttcag tacttagacc caaagatgac
     8281 agtatatgaa gcgattgtcg aagctacgaa tgggtattta agcgaacatc acattagaac
     8341 tttgttggcg ttattagatt ttaaaagaga caaggtattt cgattaatag gagatttaag
     8401 ccgaggggag cacgtgaaga tttcattatt atctctctta gtttctgagt ctgatatttt
     8461 gattttagat gaaatgacca actttttaga tataaaggct atcgaagcag tggaaaaggt
     8521 gcttgctaca tattcaggca tactgatatt tgtgagtcat gatcagtatt ttgtacaacg
     8581 tttagctact tcgattctag atattgatac ctataagata atgtaggatg gacaattaca
     8641 aatattttgg agtgaaagaa tctccctcca tcaagcatct atcaaatagt gatagtataa
     8701 aaaggacgtc cgtttgatta ggtaccctgt tttgatgaca gtttaacaaa aacaataaaa
     8761 gttttaaggt acagactact agctgtaatg ctatattatt gacgcagaga tgtgatgttc
     8821 tcatttttaa aagttgtgta gcctccgtga tcaagttaaa ggtgagtgtt caatatccat
     8881 tattaaagaa agttatttta tttttggacg gtaaaccaat gtcatcaaaa ataaaaatga
     8941 aaccatgcaa tgataattcg taatttctgt gtgaaacgta ggtaacatat gaaaaatcac
     9001 gctataaatt tcagaataat aagaaattac aaaggggtta cgtttaagga aatttctaaa
     9061 agagggatat catcattcca accctcacgt tttgataaag gggagactca cgactgcaag
     9121 aatgctacag tagttgattt ttcgaatcag cgtagaatga tgtggtcaca gttgacccac
     9181 cgcgcaaagg gtgtgacgcg acatttattg aaactttaaa cgcgttagcc ccaaaacgta
     9241 tcgtctatgt gtcatgcaat ccaagtacac aattacgaga tattgaaatg ctaagcgcgc
     9301 aatatgaact aaaagaaatg acacctgtcg atatgtttcc gcatacgaca catgtcgaga
     9361 cagttgcgtt attagtaaga aaatattgat atagatgaat cactcaaggg ttttttgaga
     9421 acatttttga gaatgtgact tagaaccctt ttttgttttg gtgctatttt aggagcgttg
     9481 atactggaac tgatttgaca gtgttaatct ctagagaatg gcgagtaatg aagttttgaa
     9541 gttattctaa atataaaata aaatcattgt tctgaatatt caaaatacac atttacatat
     9601 tctgtattca tacgctatat taaagatata agattaagaa gttggtaaaa tagcttattt
     9661 aaattattta gttttacgtg ctatctataa aatatgttca gctcagcaag aatatcctcc
     9721 attttgggtt gacatcataa actaaaatta atggcatatt aaaaaagtat ttttttacgg
     9781 aggtaaaagc aatgtaaaat ttaatcatct tgatgcaaca tagacattta ttcgtaattt
     9841 ctatatgaat tggaggtatc atatgaaaaa ttacggcaaa acttttagaa aaataagaaa
     9901 atcgagaggc ataaagctta aagatattgc aaagatgggg atatcagtct cgcagttatc
     9961 tcgctttgaa aatggagaga caaacttaac catttcgaaa tttatattaa tattaaatga
    10021 aattatgatg cccttagatg aatttatgaa tgaagcacat gatttccgaa ataatagatt
    10081 aaatgaacga tttgaacata tgatttattt tactattgag aatgacattt tacgtatgaa
    10141 acagctatta tttgatgaaa tgaagaaaaa tactcagaga agtcattttt attatttaaa
    10201 tttgatattg attaagattc gtctacaagt tatgacaggg gaattgtatt atacaaaaga
    10261 tgatttagag gttttaattg attacctttt tactaccgaa tactggggat attatgagct
    10321 gtatcttttt acaaatactt tagatgttat aaatcatgag acgatgatgc tcttatcacg
    10381 agaaatagta tcacaaacaa aggcgcttaa taaattacaa ttgtatcgtc gaatgctttc
    10441 agcaatgttg ttaaatggat atatgacttg cattcaacga caaaaaatga aagatgctat
    10501 tttttttgaa aaatgtatag agaggtgttt gtttactgaa acagaaattt acgaaaagct
    10561 tgtatttcaa tatgctaagc attttttaat ttatagtaaa tcctctagtg aggacgctat
    10621 caataaaatg aaaaaaatca ttgaagttat gcgcttagta gaaagtcatc atattgcatc
    10681 ttactatgag aatcatttaa accatatatt atgtgaaaaa aatgtgcata tatgggacag
    10741 tttgtaaaaa acattaaatt gtatattaca atgtaaatgt aggcttacaa tataattagt
    10801 aatatcagag gtggataata tgattattga gtttacaaga gaagaattgt tacagatgca
    10861 aattattgat gcaataatga aatcctctaa tagcgtttta aatgaaatga atacaagtaa
    10921 gtatattacg cggcgtcgat attagtaaaa aggtgatgga ttatggttta tgagttagaa
    10981 gaaggtgtac tacgaaaaat agaggggagt agtaatcctg tattgattcc acttacaacg
    11041 attt
//
