LOCUS       NZ_LR134263            20706 bp    DNA     circular CON 05-APR-2024
DEFINITION  Staphylococcus delphini strain NCTC12225 chromosome 1, complete
            sequence.
ACCESSION   NZ_LR134263
VERSION     NZ_LR134263.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus delphini
  ORGANISM  Staphylococcus delphini
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus; Staphylococcus intermedius group.
REFERENCE   1  (bases 1 to 2798161)
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2018) WTSI, Pathogen Informatics, Wellcome Trust
            Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900636325.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 03:36:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,651
            CDSs (total)                      :: 2,569
            Genes (coding)                    :: 2,507
            CDSs (with protein)               :: 2,507
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 22 of 62
            Pseudo Genes (incomplete)         :: 40 of 62
            Pseudo Genes (internal stop)      :: 16 of 62
            Pseudo Genes (multiple problems)  :: 13 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:43:04
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 913229
            Orig. end                         :: 933935
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR134263.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20706
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="3"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20706
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20706
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10706](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10706)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     gene            complement(689..913)
                     /locus_tag="EL101_RS04115"
                     /old_locus_tag="NCTC12225_00853"
     CDS             complement(689..913)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830019.1"
                     /locus_tag="EL101_RS04115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00853"
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="WP_096542339.1"
                     /transl_table=11
                     /translation="MKHELGTIGMVCPFPLIEAQKKMEDLNIGDELKIDFDCTQATEAI
                     PQWAAEQNYPITEFEQLGDASWTITVQKA"
     gene            complement(938..2023)
                     /locus_tag="EL101_RS04120"
                     /old_locus_tag="NCTC12225_00854"
     CDS             complement(938..2023)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167552.1"
                     /locus_tag="EL101_RS04120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00854"
                     /product="YeeE/YedE family protein"
                     /protein_id="WP_096598729.1"
                     /transl_table=11
                     /translation="MMWTIISGLTVGVLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LVAISIQAVGLFVLTSLGVLTIHNGTFPIIGTIIGSFIFGVGIVLAGGCATGTWYRAGE
                     GLIGSWIALVMYGLFAAISKFGILLPLKNVVNSQTVANADISTSTGIPSWVWTTALVSI
                     TVFLVIKTLRKPKPKVMIPKLKSRYTGVRYFLFEKRFHPFVAAIAVGLIALIAWPLSYS
                     TGREGGLGITTPSAHIIQFLTTGDFSLIDWGVFLVLGIFLGSYVAARGAKEFKWRLPDI
                     KTIRNSAIGGAFMGFGAAVAGGCSIGNGLVATAALSWQGWLGLASMILGTWFMSYFLFV
                     KPLNAARRQTQIVSTSANASH"
     gene            2257..3204
                     /locus_tag="EL101_RS04125"
                     /old_locus_tag="NCTC12225_00855"
     CDS             2257..3204
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1078: LacI family
                     transcription regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166400.1"
                     /locus_tag="EL101_RS04125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00855"
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_096598727.1"
                     /transl_table=11
                     /translation="MNIQDIANLAGVSKSTVSRYLNQGSISLKTKRKIQHVIDTYGYEP
                     NQFAQSLRAQKSMMIGIIFPRMYSHAVSQTVKGIKAKCDAFGYQMLMNLTERHVEQELD
                     ALKSFKRSKVDGILFMATTVTDAHMSVIQEIDKPVIIIGQAHASLSSVYHNDYQAGQLM
                     GREIVAQGKQHVIYASIQEDDVAVGEQRFQGLMEVLTEHHITYETLIASFDYETALQSI
                     KGQLTQQPSVAYVGATDTIALALYQALMHDHMTETPWIAGFGGDPLTQIVHPAIFTIPY
                     QYEAAGRIAFEQLQLQMEQPDLIQSRQLDVDSKC"
     gene            3260..4747
                     /locus_tag="EL101_RS04130"
                     /old_locus_tag="NCTC12225_00856"
     CDS             3260..4747
                     /EC_number="3.2.1.26"
                     /GO_function="GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166401.1"
                     /locus_tag="EL101_RS04130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00856"
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="WP_096598725.1"
                     /transl_table=11
                     /translation="MEQWSREKRYRKYEEATHEEMEILTAKVKRSPFRQTYHIQPPTGL
                     LNDPNGLIFFNGQYYIAHQWFPLGAVHGLKYWRLLTSNNLVQFEDRGVVLRPDQTFDSH
                     GVYSGSAFSFNQKLYYMYTGNHRDNEWNRHSSQIVAEVQADGSVRKLLPPAIPAPPQGY
                     TQHFRDPKVFEKDGKLYALLGAQRENETGCAVLYEAERPEGPWQFKGEIQTQLEDFGYM
                     WECPDYFQIHGKDILLFCPQGLESKGDAYQNIYQSGYIMGTLNFDTLAFDHGPFIELDH
                     GFDFYAPQTMTDEAGRRVLIGWMGLPDTEYPTDDDGWAHCLTLPRTLTIEDGKLKQKPH
                     MHLRKLRKNEEVALGYANKFIKQLHPYEGVQYELVVDILENEASAFELQLRASRNESTT
                     IRYDATTRQVSLERFDSGALPYPVEGTMRVTQLHTDLKQLRIFVDTSSIEIFCNEGERV
                     LTSRIFPKATSNKFKVVTDSGQVYLKMTKYDIVEGES"
     gene            4748..5692
                     /locus_tag="EL101_RS04135"
                     /old_locus_tag="NCTC12225_00857"
     CDS             4748..5692
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1276: PfkB domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166402.1"
                     /locus_tag="EL101_RS04135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00857"
                     /product="carbohydrate kinase"
                     /protein_id="WP_096598723.1"
                     /transl_table=11
                     /translation="MKRFYAIGEALIDFIPTQRETSLKDVTGFEPQVGGAPCNVAAAVQ
                     KLGGNSHLITQVGEDAFGDKIIETLQGVEVDTSHVLTTKKANTALAFVSLSHEGERDFS
                     FYRKPSADMLYEAKNIDTIELQQGDLIHFCSVALVDSPMKEAHLRLLDKAEQVGATIVF
                     DPNVRLPLWDDHDLYYDTLQAFLPRAHVVKVSDEELSFVTRQDDETEALKSLFVGNVQA
                     VIYTKGSRGASLIFKDGATIEVPTPPAQVVDTTGAGDAFIGAINAKLCQSEQQPIDTLR
                     QHGHDILKFAHQVSGCVVSQYGAISSLPTLEEL"
     gene            complement(5752..6468)
                     /locus_tag="EL101_RS04140"
                     /old_locus_tag="NCTC12225_00858"
     CDS             complement(5752..6468)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /locus_tag="EL101_RS04140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00858"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_019166403.1"
                     /transl_table=11
                     /translation="MKILICEDDPKQRERMVSIIENYIMIEEKPMEIELATNDPYAILE
                     TSKNMTDIGCYFLDIQLESDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKVAA
                     MDFIFKDDPEELRTRIIDCLETALKRLDLLTKDHTVETLELKRGTSSVYVNYDDVMFFE
                     SSPKSHRLIAHLDNRQIEFYGNLKELAQLDDRFFRCHNSYVLNRRNITRVDTKERIAYF
                     KNEEFCYVSVRNLKKI"
     gene            complement(6482..>7198)
                     /locus_tag="EL101_RS04145"
                     /old_locus_tag="NCTC12225_00859"
                     /pseudo=""
     CDS             complement(6482..>7198)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425575.1"
                     /locus_tag="EL101_RS04145"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00859"
                     /product="ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="ILIIATTLTIEREINYKRKKQELDDYYKYTVQIEKINNKMRKFRH
                     DYTNILLTMSEYLREDDLEGLKKYYHEHISPLKSEFESNTMRLNGIENLKVREIKGLIT
                     TKILQAQENNIEITVEVADEITQIDMDAIQLSRVLGIIMDNAIEASNTIEDPIIQIAFI
                     KTDESVMIIIMNKAPKDMPKLHTLFQDGFSTKGNNRGIGLTTLKEIIDQTDNVFLDTTI
                     ENHYFIQKLEIMNDYE"
     gene            complement(7263..8030)
                     /gene="istB"
                     /locus_tag="EL101_RS04150"
                     /old_locus_tag="NCTC12225_00860"
     CDS             complement(7263..8030)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="istB"
                     /gene_functions="other (smcogs) SMCOG1151: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095317.1"
                     /locus_tag="EL101_RS04150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00860"
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="WP_096596554.1"
                     /transl_table=11
                     /translation="MYTDHQKLLENFQQLNLKMIKDYYPKYLESLSKNQKSLTEILLEL
                     TEKEVEYQAEQKFKRAIKLARFPKVKYLNDFDFTFQPSINKQEILTLKSMHFLEKSINI
                     CFLGNSGVGKTHLAISLGVEACKQNIKTRFYTFKELIELLTTSEEKGIINKTLKQLNRI
                     ELLIIDEIGYTPISKEQADLFYQLMSLRYEMKSTIITTNIPFSSWGDSFSNKIASAAII
                     DRLIHHSKIFKITGDSYRLKDYKSEKSLNIRHS"
     gene            complement(8042..9238)
                     /gene="istA"
                     /locus_tag="EL101_RS04155"
                     /old_locus_tag="NCTC12225_00861"
     CDS             complement(8042..9238)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="istA"
                     /gene_functions="other (smcogs) SMCOG1213: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667669.1"
                     /locus_tag="EL101_RS04155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00861"
                     /product="IS21 family transposase"
                     /protein_id="WP_241971460.1"
                     /transl_table=11
                     /translation="MKGIKPNYAELARQYHCDPRTVKKYYEAGKGNELKKLKTRKTTKR
                     VSKLEPYKTLIDEKLELGCTAMAIYKYITKKGYEGKYTILREYCKNKKGKEIKKATIRV
                     ETRPGIAAQVDWKEDMVMHDKFGKRYQFNIFLYVLHYSKMKYITLTWDRKQDTLFQCLK
                     ESFEYTGGVPKEIWFDNMKTVVDRPRTQYRKVVFNTLFHQFSKDANFEPIACRPYRPQT
                     KGSVESLAKFVEQRLRPYDYEFYDAVELISLVNHFCHEMNHNEISQATDCYPIDLFNSE
                     EKHLLNPFNVQLLNTYVEDECIRIVSKESMVNFRKGKYSVPTKFIGEEVELIFNELTDK
                     LSIYFDAELIRTHHLSEKKFNYVTKDMCEILKSDAFKHKDDQEILTYIEDSLLKYDEV"
     misc_feature    complement(9861..9879)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 23.7"
                     /tool="antismash"
     gene            complement(10001..10138)
                     /locus_tag="EL101_RS04165"
                     /old_locus_tag="NCTC12225_00863"
     CDS             complement(10001..10138)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: TIGR04223"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014613413.1"
                     /locus_tag="EL101_RS04165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00863"
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="WP_096597589.1"
                     /sec_met_domain="TIGR04223 (E-value: 1.6e-05, bitscore:
                     22.2, seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRILEVLFNLITNLFQSIGSFARIPISTGFFDEPEIPSELLEEDK
                     "
     gene            complement(10140..10706)
                     /locus_tag="EL101_RS04170"
                     /old_locus_tag="NCTC12225_00864"
     CDS             complement(10140..10706)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014613414.1"
                     /locus_tag="EL101_RS04170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00864"
                     /product="accessory gene regulator AgrB"
                     /protein_id="WP_096542351.1"
                     /sec_met_domain="AgrB (E-value: 4.1e-31, bitscore: 105.0,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLIDNGIEKMALKLQQRQNLSHIEFLKVRLGMQVVVINSFKAVV
                     TYGLALLLNIFLYTLIVHLTFLALRGYSHGAHAKTSMLCHVQNIASFVVLPWLIVQYDI
                     SFQFLLSLSILAAMIVIKYAPAATKKRPIAPRRVKGLKIKSIIVFILLMALAFIVPTPY
                     NRFVVYGVLLQSFTLLPIFSTKEEV"
     gene            complement(11459..12262)
                     /locus_tag="EL101_RS04180"
                     /old_locus_tag="NCTC12225_00866"
     CDS             complement(11459..12262)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166407.1"
                     /locus_tag="EL101_RS04180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00866"
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="WP_096597591.1"
                     /transl_table=11
                     /translation="MQIQLFQFNVAIANSRQNEQKIATLFEQHLDNQTEVIVLPEMWNN
                     GYALPQLEQLADEDLAQSYAFIQKLAQHYHVDIVAGSVSNHKEGAIYNTAFAVNRHGEK
                     IYEYDKIHLVPMLDEPDFLTGGETVPYPYTLSNGTSVSQIICYDLRFPELSRYPAAQGA
                     KVMFYVAQWPEVRLTHWRQLLQARAVENDMYVVAVNGSGDDGKTTYAGHSIVVDPNGEI
                     IAEADENETVMTVTLDLQKVDTQRRAIPVFENMRPSVYRYAQKEI"
     gene            12427..13791
                     /locus_tag="EL101_RS04185"
                     /old_locus_tag="NCTC12225_00867"
     CDS             12427..13791
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014613417.1"
                     /locus_tag="EL101_RS04185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00867"
                     /product="SdrH family protein"
                     /protein_id="WP_096597593.1"
                     /transl_table=11
                     /translation="MVEHKKEHSVKRLFKLGIGSMSILCVVSPLLLTRDVVQAANINNS
                     ATTLNTLRVTSSYDQTADMDDLRSDVSSNGNAAETPSFNEITTPSTDETDAASTENVKP
                     NDEVPATDESESTTPSTEDDTSIEEPDTEEVPSHEENQHHNDPSQEGQPSQPDQPDTKK
                     DEESGEQPNKENHQKPHQPNKDKPSKDQHTKPDKEDKPTPPPKVPDRPNQKDDTSNHIP
                     PPATDNGGNGNGGTTTGPNGGGGSEASPQPNGQPSNGNASDPQQNGSVASTNHSNQYGT
                     SAYDEYAGLLNNNYKYNPLFKEEVARLSQFGSQGQRDIASLSRKEQFSQNAFLDDLQQS
                     TDYFRYQYFNPLSTEQYYHRLDKQVLELVTGEFGSMPDFKKSGDKSLVNKHQQDKVKKI
                     EQHGENFNTHRMKDTKEDAGKSLSYKPMIYIGIVMVGFVGLISMILWKRLHHFWK"
     gene            complement(13903..14670)
                     /locus_tag="EL101_RS04190"
                     /old_locus_tag="NCTC12225_00868"
     CDS             complement(13903..14670)
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166409.1"
                     /locus_tag="EL101_RS04190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00868"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_096597595.1"
                     /sec_met_domain="Abi (E-value: 7.1e-16, bitscore: 55.7,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRFWVSLLTVVLYILAQISPFIARAVGWIQTDNQAELLRQSIAV
                     QFTAFIIVAILIILLQFFVKNKLNFERGPKEKKRYVLPYILVGLVIVFIAQMIVNLISV
                     QLFGANPASENTLRIMKIARQMPIFIVLIAIVGPLLEEFVFRKVLFGELYHAIKASRWV
                     KFTIATTISSTVFAVVHMDFSHFLAYFVMGIIFSAFYIYTKRLSVSIGIHMAQNGLVAL
                     IQLMIPEKMIEEAVKQTQFVHLLTTQWMSFFLS"
     gene            14840..15127
                     /gene="groES"
                     /locus_tag="EL101_RS04195"
                     /old_locus_tag="NCTC12225_00869"
     CDS             14840..15127
                     /codon_start=1
                     /gene="groES"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275309.1"
                     /locus_tag="EL101_RS04195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00869"
                     /product="co-chaperone GroES"
                     /protein_id="WP_019166410.1"
                     /transl_table=11
                     /translation="MLRPLGNRVVIEKKEHEQTTKSGIVLTDSAKEKSNEGVIVAVGPG
                     RTLDNGERLKPELNVGDRVVFQQYAGTEVKRDDTEYLVLTEDEVLAVIED"
     gene            15177..16796
                     /gene="groL"
                     /locus_tag="EL101_RS04200"
                     /old_locus_tag="NCTC12225_00870"
     CDS             15177..16796
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /gene="groL"
                     /gene_functions="other (smcogs) SMCOG1299: chaperonin
                     GroEL"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /locus_tag="EL101_RS04200"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES; misfolded
                     proteins enter the barrel where they are refolded when
                     GroES binds; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00870"
                     /product="chaperonin GroEL"
                     /protein_id="WP_019167564.1"
                     /transl_table=11
                     /translation="MAKELKFSEDARQAMLRGVDKLANAVKVTIGPKGRNVVLDKEFTA
                     PLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEGLK
                     NVTSGANPVGIRQGIDKAVAVAIESLHNISQKVENKEEIAQVGAISAADEEVGRYISEA
                     MEKVGNDGVISIEESNGFNTELEVVEGMQFDRGYQSPYMVTDSDKMIAELERPYILITD
                     KKISSFQDILPLLEQIVQSNRPILIVADEVEGDALTNLVLNRMRGTFTAVAVKAPGFGD
                     RRKAMLEDLAIVTGAQVITDDLGLELKEATLDMLGTANKVEVTKDNTTVVDGDGDPANI
                     DARVGQLKAQIEETDSDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALN
                     STRAAVEEGIVAGGGTALMNIFKDVQAIEAEGDEATGVNIVLKALQAPVRQIAENAGLE
                     GSVIVERMKNAEPGIGYNAATDEWVNMLEAGIVDPTKVTRSALQHAASVAAMFLTTEAV
                     VANIPEDKGNDMPQMGGMPGMM"
     misc_feature    complement(15193..15207)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.86"
                     /tool="antismash"
     gene            17069..17674
                     /locus_tag="EL101_RS04210"
                     /old_locus_tag="NCTC12225_00871"
     CDS             17069..17674
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368755.1"
                     /locus_tag="EL101_RS04210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00871"
                     /product="DUF3885 domain-containing protein"
                     /protein_id="WP_096597597.1"
                     /transl_table=11
                     /translation="MSNINSCMEVSGLKFSEGENAVHLDLSEGEYPFLDDGKSFNKDYF
                     ERIHNNAIQILQDLFQQSNTIDIVLLYYLYGDSFKKTRFKEKFSYFNNNEIPDFKEYIN
                     DEGIQCYIFSYKNKTLKDLNYKKLVRAISNQDFKGLFPTINQKDNYLDIYLMDSKRGIL
                     FHLYDDRGLWLYFLNKQSYKIYSQKYSNLLFDVSHEMD"
     gene            17661..18362
                     /locus_tag="EL101_RS04215"
                     /old_locus_tag="NCTC12225_00872"
     CDS             17661..18362
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015729408.1"
                     /locus_tag="EL101_RS04215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00872"
                     /product="S8 family serine peptidase"
                     /protein_id="WP_096597599.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 3.5e-14, bitscore:
                     50.0, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKWIKVYLKLIILILLSVFIYLNYNCKYEKSKIAVLDTGVEKKAI
                     KSKVISRNFTLDNNWNSVHANKVSKIIEYEADIVIYDAKVLNRHGNGRLEDTISALDWA
                     IKNNVDIINMSYGFTKNYPELHRKIKEAHEKGIIIVAANGNDIFGGKEFPASYKETISV
                     GVLNKEGSKSVFNSNDDADVFISVDNKLPNSGENTSMATAYVSNKLTKLCNRNLRNMDK
                     DEILKLINELD"
     gene            complement(18592..19824)
                     /locus_tag="EL101_RS04220"
                     /old_locus_tag="NCTC12225_00873"
     CDS             complement(18592..19824)
                     /EC_number="3.5.1.16"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1247: acetylornithine deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166413.1"
                     /locus_tag="EL101_RS04220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00873"
                     /product="acetylornithine deacetylase"
                     /protein_id="WP_096597601.1"
                     /transl_table=11
                     /translation="MEQRQFDILQTLIAHPTVSPPARNTQTLQQQISTWLENTGFDVQT
                     IPFYDNDTIIVGTLKGRNPDAPRLILNGHVDVAEVEDEQFWRFNPFQLTEVDGFLFGRG
                     VADMKGGMSALFYNLERLHQEGLQPEGDIIVHSVVGEEVGEAGTKVACEHSPKADLALV
                     LDTSDNIAMGQGGVITGWVTIQSQETIHDGARSHIIHAGGGRHGASAIEKMVKIIQALQ
                     ELERHWAVTKSCPGMPPGANTINPAVIEGGRHPAFIADQCRLWITVHYLPDENYETIIE
                     EIETYLNKVAESDLWLNENPLQFEWGGTSMIEDKGEIFPSFTLPTTHAGFHMLAKAHEH
                     VHQTPLQTTMSTTVTDGGWTADFGIPTILYGPGELNEAHGTNEKIRIQDLDHFTEVLYA
                     FLKEWYEHPVR"
     gene            complement(19805..20326)
                     /locus_tag="EL101_RS04225"
                     /old_locus_tag="NCTC12225_00874"
     CDS             complement(19805..20326)
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014613423.1"
                     /locus_tag="EL101_RS04225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC12225_00874"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_096597603.1"
                     /transl_table=11
                     /translation="MSFYFQHINTPLGLMTAVVNDNALLGLSFTDSKDYHTALKHFQKQ
                     APLVQISHHPIVNQIALELEAYFHGHYHTFKTPVAHVIGTPFQQQVWQALQQLPFGAVV
                     NYSDIAQMIGRPKSVRAVASAIGQNPLSIIVPCHRVLRKDGQLGGFNSGLHRKKYLLAM
                     EGHPYGTTTI"
ORIGIN
        1 agcgaaaggc gaagatcgtg ttaattcaaa agccattagt gaaggcttaa atatagattc
       61 agccacaata cgtcgagatt tttcttattt tggtgaatta ggcaaaaaag gttacggtta
      121 taatattgat agtttattag aattttttaa atcagaatta agtgattcag atattatcaa
      181 aattggtatc gtcggtgtcg gtcacttagg tacagcatta atttcgtata acttttcaat
      241 ccatgatgat atgaccatta cagaagcctt tgacattaga gaagacatca tcggtacgac
      301 agttggggat attacgatta agtcgatgga tgacattaaa gaaaccattg cttcaggaga
      361 attagaagtt gtcataattg cgacacctga atcagcagca caggcagtga ccgatcaatt
      421 agtcgaagca ggtatcaaag gtatcttgaa ttttacgcct aaacgtgttc acgcgccaca
      481 atcagtacaa gtacatcaaa ttgacttagg gattgaactc caatcgctac tcttctttat
      541 gaaaaattat ggtgtaacgg aagaataaaa attgacgaac tgagcttata aacaatagct
      601 cagttgagcg tttgctaata atgactagaa cacaaaaaag caacgtcctt gtgaataagg
      661 taggcgttgc ttttttgtgt gtcaatcatt aagctttttg aacggtaatg gtccaagaag
      721 catcacctaa ttgctcgaat tcagtaatgg gataattttg ttctgctgcc cattgaggta
      781 tagcttctgt agcttgtgtg caatcgaaat ctattttcaa ctcatcacca atatttaaat
      841 cttccatttt cttttgtgct tctattaatg ggaaagggca taccattcca atagtgccta
      901 attcatgttt cattttttat cacctttctt taataattta atgagatgca tttgctgaag
      961 tactaacaat ttgcgtttga cgtcgtgcgg catttaatgg ttttacaaat aagaaataac
     1021 tcatgaacca tgtccctagt atcattgaag ctaaacctaa ccatccttgc catgaaagtg
     1081 ctgctgtagc aactaagcca ttaccgatag agcaacctcc agcgactgct gcaccaaacc
     1141 ccataaaagc gccgccaata gcactgttac gaatggtttt aatatctggt agacgccatt
     1201 taaactcttt tgcaccacgc gcagcaacat aagaacctaa gaaaattcct aaaacaagaa
     1261 agacacccca atcgattaga gagaaatcgc ctgtagttaa aaattgaatg atatgtgcag
     1321 aaggtgtcgt aatacctaac ccaccttcac gaccagttga ataactcagc ggccaagcga
     1381 ttaacgcaat tagtcctact gcaatcgctg caacaaatgg atggaaacgt ttttcaaata
     1441 aaaagtaacg tacacctgta taacgtgatt ttaatttcgg aatcatcact ttaggttttg
     1501 gcttacgtaa agttttgatg actaaaaata ctgtaatact tactaaagcg gtggtccaaa
     1561 cccagctcgg tataccagtt gaagtagaaa tatctgcatt agcgacagtt tggctgttaa
     1621 ccacattttt cagtggtaat aaaatgccaa attttgaaat cgcagcaaac aatccataca
     1681 tcactagtgc aatccaactt cctataaggc cttcaccagc acgataccac gttcccgttg
     1741 cacatccacc agccaataca atacctacac caaaaataaa tgatccaatg atggtgccga
     1801 taatagggaa ggtaccatta tgtatcgtca acacacctaa ggacgttaaa acaaataacc
     1861 cgaccgcttg tattgaaata gcaaccagta aggcatagaa cattttattg tttttttgca
     1921 catacatatc tctaaagcct cctgttaggc aaaatcgtgt acgttgcatc acgaaaccga
     1981 gtaaaacccc aacagttaaa ccactaatta ttgtccacat caaaataact cctttctata
     2041 tccgactatt taaataggta ttatatggta tatgtaaaaa gaatgcaata taatccataa
     2101 aataattttg gaaaatacgc atgcgtcact acatactgaa attgctcact aagacttgaa
     2161 tgctacactt tatttttaca ctgcatcgta ttttatacag taatcatgag gggttttatg
     2221 ataaaatagc tattgagact gtgaggtgat gaacaattga atatacagga tattgccaat
     2281 ctagcgggtg tttctaaaag tacagtttca aggtatttga atcaagggtc gattagttta
     2341 aagacaaagc gaaaaattca acacgtcatt gatacatacg gttatgaacc gaaccagttt
     2401 gctcaaagtt taagagcaca aaaaagcatg atgattggca ttattttccc gcgaatgtat
     2461 tctcatgccg tgtcccaaac ggttaaaggg attaaagcga aatgtgacgc atttggctat
     2521 caaatgttaa tgaatttgac tgagcgtcat gtggaacagg aactggatgc gctgaaatct
     2581 tttaaaagaa gtaaagtaga tggcatttta tttatggcga ctactgtgac agatgcacat
     2641 atgtctgtca ttcaagaaat tgataaacca gttatcatca ttggtcaagc acatgcgtcg
     2701 ttaagctcgg tttaccacaa tgattatcaa gctggtcaat taatgggacg ggaaattgtg
     2761 gcacaaggga agcaacatgt catttatgcg agtattcaag aagatgacgt cgcggttggt
     2821 gagcaacgtt ttcagggttt aatggaagtg ttaaccgaac atcacatcac atatgagaca
     2881 ttgatagcat cgtttgatta tgaaactgca ctacaatcga ttaaagggca gctcacgcaa
     2941 caaccatctg tggcgtatgt cggtgcgaca gatacgattg cgttggcgct ctatcaagcg
     3001 ttgatgcacg accacatgac agaaacgccg tggattgctg gttttggcgg agatcctctg
     3061 acacaaattg ttcatccagc gattttcacc ataccttacc aatatgaagc ggcaggacgt
     3121 atcgcatttg aacaattaca gcttcaaatg gaacagccag atttaattca aagtcgtcaa
     3181 cttgacgtgg attccaaatg ttgaggattt atcttaaaag aggtggaacc gttaccaaat
     3241 cgttaaaagg aggtacatca tggaacaatg gagtagagaa aagcgttatc gaaagtatga
     3301 agaagcaaca catgaagaaa tggagatatt aacggccaaa gtgaagcgct cgccatttcg
     3361 acaaacctac catattcagc ccccaacagg attattaaat gatccaaacg gcctgatctt
     3421 ttttaatggt caatattata tcgcacatca atggtttccg ttaggtgcag tacatggttt
     3481 aaaatattgg cgtttgctta caagtaacaa tctcgtacag tttgaagatc gtggtgtcgt
     3541 attacgtcct gatcaaacgt ttgatagtca tggcgtgtac agtgggagtg cattttcgtt
     3601 taatcaaaaa ttgtactata tgtacacagg taatcaccgt gacaatgagt ggaaccgtca
     3661 cagtagtcaa atcgtagcag aagtacaagc agatgggtcg gttcgtaaat tgttacctcc
     3721 agccattccg gcacctcctc aagggtatac acagcacttt cgcgatccta aagtgtttga
     3781 gaaagatgga aaactgtatg cattgctagg ggcacaacgt gaaaatgaaa cagggtgtgc
     3841 cgtattatac gaagctgaac gccctgaagg accgtggcag tttaaaggtg aaattcaaac
     3901 gcaattagaa gattttggtt atatgtggga atgtccagat tattttcaaa ttcatggaaa
     3961 agatattttg ctattctgtc cacaaggctt agaatctaaa ggcgatgcgt atcaaaatat
     4021 ttaccaaagt ggttatataa tgggaacgtt aaattttgat acgttagcat ttgatcatgg
     4081 tccgtttatt gaacttgacc acgggtttga tttttatgcc ccgcaaacga tgactgatga
     4141 agcgggacga cgtgtgttaa tcggttggat gggtctacct gacaccgaat atccgacaga
     4201 tgatgacggt tgggcgcact gtttaacttt gccaagaaca ttgacgattg aagatggtaa
     4261 attaaaacaa aaaccacata tgcatttaag aaagttacgt aaaaatgaag aagttgcgct
     4321 cggttacgcg aacaaattta ttaaacaact ccatccttat gaaggggtgc agtatgaact
     4381 tgtcgttgat attttagaaa atgaggcttc tgcttttgaa ttacaactcc gtgcatcacg
     4441 caatgaatcc acaacgattc ggtatgatgc aacgacacgt caggtttcat tggagcgctt
     4501 tgatagtggg gcattaccgt atccagttga aggaacgatg agggtgacgc aattacatac
     4561 ggatttgaag caattacgta ttttcgtgga tacatcaagc attgaaatct tttgtaatga
     4621 aggggaacgt gtgttgacgt cacgtatatt ccctaaagca acatcaaaca agtttaaagt
     4681 cgttaccgat tcaggtcaag tgtatttaaa aatgaccaaa tacgatattg tggaaggaga
     4741 atcataaatg aaacgttttt atgcaatagg tgaagcactc atcgatttta ttcctacaca
     4801 aagagagacg tcattaaaag atgtgacagg gtttgaacca caagtcggtg gtgcgccttg
     4861 taacgtagct gcagctgttc aaaaattagg tggcaacagt catttgatca cacaagtggg
     4921 ggaagacgct tttggtgaca aaatcatcga gacgttacaa ggggttgaag tcgacacctc
     4981 acatgtttta acgacaaaaa aagcgaatac agcacttgca tttgtcagtt tatctcatga
     5041 aggggaacga gacttttcat tttatcgaaa accgagtgca gacatgctgt atgaagctaa
     5101 aaatatcgac accattgagt tgcaacaagg tgatttaata cacttttgtt cggttgcatt
     5161 agtcgatagt cctatgaaag aagcgcattt aagattactg gataaagcgg aacaagtcgg
     5221 cgcaacgatt gtgtttgatc cgaatgtacg cttaccttta tgggatgacc atgatcttta
     5281 ttacgacaca ttgcaagcat ttttaccacg cgcacatgtg gttaaagttt cagatgagga
     5341 actgtcattt gttacacgac aagatgatga aacggaagca ttaaaatcac tgtttgtcgg
     5401 caatgttcaa gcagtcattt acacgaaagg tagtcgcggg gcatctttaa ttttcaaaga
     5461 tggcgcaaca attgaagtgc cgacaccgcc agcacaagtg gtggatacta caggcgcagg
     5521 agatgcattt atcggtgcta tcaatgcgaa attatgtcag tctgagcaac agcctattga
     5581 tacgctacgc caacacgggc atgatatttt gaaatttgca catcaagttt caggttgtgt
     5641 cgtgtcgcaa tacggcgcca tttcaagctt accaacatta gaagaacttt aattaaaaag
     5701 ccaagttccc ggataggtgg gagattggct ttttaatgcg agatagcgct tttaaatttt
     5761 cttcaaatta cgcaccgaga cgtaacaaaa ttcttcattt ttgaaataag caatccgttc
     5821 tttcgtatcg acacgcgtta tattccttct attcagcaca taactgttgt gacatctgaa
     5881 gaaccggtca tcgagttgag ctaactcttt caaattgccg taaaactcga tttgacgatt
     5941 gtctaaatgc gcgattaaac gatgtgactt cggtgaggac tcgaaaaaca tcacgtcatc
     6001 atagttcacg taaaccgagc tcgtcccacg tttgagttcc aaagtttcaa cagtatgatc
     6061 ttttgttaat aaatcaagcc gttttaatgc tgtttctaaa caatcgataa tgcgtgttct
     6121 taattcttca ggatcatctt taaaaataaa atccatggca gccactttat agacaaacgt
     6181 caagtacgtc agttcactat gactcgtcac aaaaataata ttcccgacag ggtcgtgttt
     6241 acgaatttcg ctccctaatt taatcccatt aatatcagac tctaattgaa tatctaaaaa
     6301 gtagcagcca atatcagtca tgtttttaga agtttctaaa atagcgtaag ggtcattagt
     6361 agcaagctca atttccatcg gcttctcttc aatcataata tagttttcaa taatggacac
     6421 catgcgttcg cgttgtttag gatcatcctc acaaatcaaa attttcatga tacacacatc
     6481 cttattcgta atcgttcata atctctagtt tctgaataaa ataatggttt tcgatcgtcg
     6541 tatcaaggaa tacattgtcc gtttgatcaa taatctcttt taatgttgtt aatcctatac
     6601 ctcgattgtt tcctttagtt gaaaagccat cctggaacaa ggtatgtagc ttaggcatat
     6661 cttttggcgc tttgttcata ataataatca ttacggactc atccgtctta ataaatgcga
     6721 tttgaataat agggtcctca atcgtattgg aagcttctat cgcattatcc ataataatac
     6781 ctagtacacg gctaagttga atcgcatcca tatcaatttg cgtgatttca tccgccactt
     6841 caacagtgat ttcaatatta ttttcttgcg cttgtaaaat ttttgtcgta attaagcctt
     6901 taatttcacg gacttttaga ttctcaatac cattcagtcg catcgtattg gattcaaatt
     6961 cgctttttaa aggactaata tgttcatgat aatatttctt caaaccttct aaatcatctt
     7021 cacgtaaata ttcggacatc gttaataaaa tattcgtata gtcatgtcgg aatttacgca
     7081 ttttgttgtt aattttttca atttgtactg tatatttata ataatcatct aattcttgct
     7141 ttttccgttt ataattgatt tcgcgttcta tcgttaaagt ggttgctata attaatatag
     7201 ctgtcaatag cggtttgaaa atgttgtttt atggcggttt gaaaatgtcg ttttttggcg
     7261 gtttaagaat gacgtatgtt taaacttttt tcacttttat agtctttaag tcggtatgaa
     7321 tcgcctgtaa ttttaaatat ttttgaatgg tgaataagtc tatcaataat ggctgctgac
     7381 gctatcttgt tactaaatga gtcgccccaa ctagaaaaag ggatattcgt cgttatgatc
     7441 gtggatttca tttcatatct tagtgacatc aattgataga agaggtctgc ctgttctttt
     7501 gaaatgggtg tatagcctat ctcatcaata ataagtagtt caattctgtt taattgtttt
     7561 aaagttttat tgatgattcc tttctcctct gaagtggtta agagttcaat taattcttta
     7621 aaagtataga atcgagtttt gatattttgt ttacaagctt ctacgcctag cgatattgct
     7681 agatgtgtct taccgacacc actattacct aagaagcata tattgatact cttctctaga
     7741 aaatgcatgg attttaatgt gagtatttct tgtttattaa tgcttggctg aaacgtgaaa
     7801 tcaaagtcat ttaaatattt aaccttaggg aaacgtgcta acttaatagc acgtttaaat
     7861 ttttgttcag cttgatattc tacttccttt tctgtcaact caagtaaaat ttcagttaaa
     7921 gatttttgat tcttggatag tgattctaaa tacttcggat aatagtcttt aatcattttt
     7981 agattgagct gttgaaagtt ttctaatagc ttttgatggt ctgtatacat atctgtttct
     8041 cctatacctc gtcatatttt aataatgaat cttcgatata cgtaagaatt tcttgatcgt
     8101 ctttgtgctt gaatgcgtca gacttcaata tctcacacat atctttggta acataattga
     8161 atttcttttc cgataaatga tgggttctaa ttaactcggc atcaaagtag atggataatt
     8221 tgtcagtcaa ttcattaaag attaattcaa cctcctcacc aataaacttt gtaggtactg
     8281 aatatttacc ttttctaaaa ttaaccatcg actctttaga aacaattcga atgcattcat
     8341 cttcgacata ggtgtttagt aactgcacat taaacgggtt caatagatgt ttttcttcag
     8401 aattgaataa atcaattgga tagcagtcag ttgcttgcga tatttcgtta tggttcattt
     8461 catgacaaaa gtgattgaca agactgataa gttcaacagc gtcataaaat tcgtaatcgt
     8521 agggccttaa acgttgttcc acaaatttag ctaaagattc cacagagccc ttggtttgcg
     8581 gcctataagg cctacacgcg atgggttcaa agtttgcatc tttactaaat tgatgaaaaa
     8641 gggtgttaaa taccactttt ctatattgtg ttctaggacg atcaacgacc gttttcatat
     8701 tatcgaacca tatttcctta ggaacacctc cagtgtactc aaaagattct ttcaaacatt
     8761 gaaataatgt atcttgtttt ctatcccaag ttaatgtgat atacttcatt tttgaatagt
     8821 gtagaacgta aagaaagata ttaaattgat aacgtttccc aaatttatca tgcatgacca
     8881 tatcttcttt ccagtctact tgagcagcta taccggggcg tgtttctacc cgtatagttg
     8941 cttttttaat ttcctttcct tttttattct tacaatattc tctaagaatc gtatatttgc
     9001 cttcatatcc ttttttagta atatatttat aaatcgccat agccgtacaa cctagctcta
     9061 atttttcgtc tatgagcgtt ttatagggtt ctaattttga tactctcttt gtcgttttcc
     9121 ttgtttttaa ttttttcaac tcattgcctt tcccagcttc ataatatttt tttactgtcc
     9181 ttggatcaca atgatattgt ctagccagtt cagcgtaatt tggttttatg cctttcataa
     9241 tgtaaaatga cactccttcg tatatatcat gtctcaatca aacaacctcc attcaaagat
     9301 tgagttaaga catgattata tattttagaa aataaaaaat gtaggaaaaa cagcattttc
     9361 attccgccat tttcctacat tttataaccg ccatttacaa tagcgaaaac cgaaaaagaa
     9421 ataaaataaa tgataccaaa atttcgaaac tcatataagt ccccgacagt tttattcaat
     9481 aacgagaaaa agcttagaaa tcctgaagct aaaaacaaac ttagaacaat caaataaaat
     9541 ttgttaatat acaaccaaga tgttttaagt ttattaaaca atgtaataat aataaaagaa
     9601 aaaatatagc aacctagcgc aaaggctatc atatagatta atgatgctac ataaaaattg
     9661 atgtgatatg tattaagata tgcaaaaaac caagtcgcga cgaaatcatt aatcattaaa
     9721 acaagaattg taagtaacac tgtaataatt ccaatgattt ttgctcgtct aaaatagaag
     9781 atgaaaaagc aaagaattaa ataaagcaaa ctatttttat cgaaaattaa atataaaata
     9841 atagaaggga ttactatccc gaaggtgaga ataatgtaat ccctttgact atatttatat
     9901 ttttggataa tactcgttac tgatgcgaaa ataaaggatt gaaataatac tataaatatg
     9961 gctaaaagta tttccaattt tatacacact ttctaacttt ttatttgtcc tcttctaaca
    10021 attcagaagg gatttctggc tcatcaaaaa atccagtact aatagggatt ctagcaaagc
    10081 taccgattga ttggaataaa tttgtaataa ggttgaataa aacttctaaa attctcatgt
    10141 taaacttcct ccttagtaga aaagataggt aataatgtga atgattgtaa taatacccca
    10201 taaacgacaa atcgattata aggcgttggt acgatgaatg caagagccat gagtaaaata
    10261 aatacaatga ttgatttgat tttaaggcct ttcactcttc ttggagctat tggtctcttt
    10321 ttcgttgcag caggtgcata tttgattacg atcatcgcag ccaatatact caagcttaac
    10381 aaaaattgaa acgaaatgtc atattgtacg attaaccatg gtaaaacaac gaatgaagca
    10441 atattttgta catgacaaag cattgacgtt ttcgcgtgtg caccatgtga ataacccctc
    10501 aaggcaagaa aagttaggtg aacaataagt gtgtataaaa aaatgttcaa taggagtgca
    10561 agtccgtaag tcacaacagc tttaaaggag ttaatcacga caacttgcat gcctaaacgc
    10621 acttttaaaa actcaatatg acttaaattc tgtcgttgtt gaagcttcag cgccattttt
    10681 tcaattccat tgtcaatgag taacatacgg ctctcctcct tgtctaaatt ataatacaca
    10741 actattaaaa cgctttactt tttgcctaac tgttcattta tcattcctaa ctgttgcaaa
    10801 tgattggttt ttaatatttt accataaatt tttaaacagt taagcataaa aaataacgag
    10861 ttacgcactc gttaacacaa aggtaaaaaa tcatgttgta atatagacat catcacattg
    10921 gtgatggaga tgaagtgatt ttgtattaag tgttgtcgag tacacttaag caaatttact
    10981 gattaagtta agtcagatgt tcttagtcat tgtattcttc gctcagctta catgactaag
    11041 ggagcaacaa tctattggag tctttggttt tatttataat ttaaggaggt gctcactatg
    11101 gcagcagata tcattagcac aatcgttgaa tttgttaaac ttatcgcaga aacagtagca
    11161 aaatttatca aaaaataaga tttgataaaa gtatttgtta gaatcttttt ccttacttag
    11221 actaaagtaa aatcaggttt cgatattgaa gccattgtag caatatttaa taatcggtaa
    11281 attcactaaa ttttttcata attaataaca tccccaatta aatagaataa ctgtaaataa
    11341 aacattccct taatatgaag aacaggcgag tccctcccaa gctcgtctgt tcatttttat
    11401 tttatggtca tgaaaaaagg cgttacttct ttaaataaga gaggtagcgc ctcatatttt
    11461 atatttcttt ttgtgcatag cgatatacgg acggtcgcat attttcaaaa actggaatag
    11521 cacgtcgttg tgtgtctact ttttgaagat ctaatgtgac ggtcatcact gtttcatttt
    11581 catccgcttc agcaatgatt tccccatttg gatctactac tattgaatgt cctgcatacg
    11641 ttgttttacc atcatcacca ctgccattaa cagcaacgac atacatatca ttttcaactg
    11701 cacgtgcttg taataactga cgccaatgtg tgagtcgaac ttcgggccat tgtgcgacat
    11761 aaaacatcac tttcgcacct tgtgctgcag ggtaacggct caattcagga aagcgtaagt
    11821 cataacaaat gatttgagat acacttgtgc cgttagaaag cgtatatggg taggggacag
    11881 tctcgccacc ggttaaaaag tctggttcat ctaacatcgg aacgagatgg attttatcgt
    11941 attcataaat tttttcaccg tgtcgattta cggcaaacgc tgtattgtaa atggcacctt
    12001 ctttatggtt agaaacagag cccgcaacaa tgtctacatg ataatgttgt gctaattttt
    12061 gtataaaagc ataactttga gctaagtctt catctgctaa ctgttctaac tgaggtaatg
    12121 catagccgtt attccacatt tcaggcaaaa ctatgacctc tgtttgatta tctaaatgtt
    12181 gttcaaataa cgtagcgatt ttttgttcgt tttgacgtga attagcgatt gcgacattaa
    12241 attgaaataa ttgtatttgc atcatcatca tccttatttt cgaattaatc ctagtgtact
    12301 aaatgatgaa cggtttaaca aaaatcttaa ttgaatacaa tcaaatatac atgagggtgg
    12361 taatcggagt cacttttgtt tatactggct ttgttaaaaa actttaagca atcagaggtg
    12421 aatctgatgg tagaacataa aaaagaacat agcgtcaaac gactatttaa attgggaatc
    12481 ggttcaatga gtattttatg tgttgtatca cctcttttat taactcgtga cgtcgttcaa
    12541 gcagcgaata tcaataacag tgcgacgact ttgaatacat tgagggtcac ttcatcgtat
    12601 gatcaaacgg cagacatgga tgatttacga agcgacgttt cttcaaatgg gaacgctgct
    12661 gaaacacctt ctttcaatga aataacaacg ccttcaactg atgaaacaga tgccgcgtca
    12721 acggaaaatg tgaaaccgaa tgatgaggta ccggcaacgg atgaaagtga atcaacaaca
    12781 ccgagtacag aagacgacac atctatagaa gaaccggata ctgaagaagt tccatctcat
    12841 gaagaaaatc aacatcacaa cgacccaagt caagaaggcc aaccgtctca acctgatcaa
    12901 ccagatacaa agaaagatga agagagtggg gaacagccga ataaagaaaa tcatcagaag
    12961 ccgcatcaac cgaataaaga caaaccttca aaagatcaac atactaaacc tgataaagag
    13021 gacaaaccca caccaccgcc taaagtgcca gatcgtccga atcaaaaaga tgatacttca
    13081 aaccatatcc caccacctgc aacagataac ggtggaaacg gtaatggcgg tacaacaaca
    13141 ggtccaaatg ggggaggtgg cagtgaagca agtccacaac cgaatgggca accgtcaaat
    13201 ggcaatgcaa gcgatcccca gcaaaacggt tccgttgcaa gcaccaacca ttcgaatcag
    13261 tatggtacat cggcttatga cgaatacgca gggttattga ataataatta taaatataat
    13321 ccattgttta aagaagaggt tgcgcgttta agtcaatttg gaagtcaagg ccaacgtgat
    13381 attgcgagtt tgagtcgtaa agaacaattt tcacaaaatg catttttaga tgacttgcaa
    13441 caaagtacag attattttag atatcaatat tttaatccgc tttctacaga gcaatactat
    13501 caccgtttag ataaacaagt attagaactc gtcacagggg aatttggttc gatgccagat
    13561 ttcaagaaaa gtggcgataa gtcattggtt aataagcatc agcaagataa agtgaagaaa
    13621 attgaacagc acggagaaaa ttttaatacg catcgtatga aagatacgaa agaagatgca
    13681 ggaaaatcat taagttacaa gccaatgatt tatattggca ttgtcatggt cggttttgtc
    13741 ggcctgatta gtatgatttt atggaaacgg ctgcatcatt tttggaaata aaataacaaa
    13801 gggttaggac gctttaactg aagtcgtctt aaccctttgt gcattgtaaa aaatatatct
    13861 gaactattta aaagtaattt tgtgaagcca accgtgtatg gtttatgaaa ggaaaaatga
    13921 catccactgt gtggttaata aatggacgaa ttgtgtttgc ttaacggctt cttcaatcat
    13981 cttttcaggt atcataagtt gaatgagtgc aactaaacca ttttgagcca tatgaatacc
    14041 gatcgacaca ctcaaccgct tcgtataaat ataaaatgcg gaaaaaatga tccccataac
    14101 aaaataagct aagaaatgcg aaaaatccat atggacaaca gcaaacactg tagaactaat
    14161 cgtcgttgca atcgtgaatt tgacccatcg actcgcttta atcgcgtgat acagttcacc
    14221 aaacaatact tttctaaaga caaactcttc taatagtggt ccgacaatcg ctatcaacac
    14281 aataaatata ggcatttgac gagcgatttt cataattctt aacgtattct cactcgcagg
    14341 attagcccca aatagttgga cagaaatgag attcacaatc atttgtgcga taaacacaat
    14401 gactagacct actaaaatat agggaagtac ataacgtttt ttctctttcg gaccgcgttc
    14461 aaagtttaat ttatttttca caaaaaactg cagtaagatg attaaaatgg cgacaatgat
    14521 aaaagcagtg aactgaactg ctatcgattg ccgcaacaat tctgcttggt tgtccgtttg
    14581 aatccagcca accgcacgtg caataaaagg tgatatttgt gcaaggatgt ataatactac
    14641 ggtgagtagt gaaacccaaa atctcttcat aattagcctc cgttcaatcc cttttattct
    14701 aacggaaaat aaaggtgtgg acaatggctt tgaacgtaaa cgcttgaaat tttgaccaat
    14761 tttgattatt atagaactaa attagcactc aataaatgaa agtgctaaat tgatgttgaa
    14821 cataaggagg cactcacaca tgcttagacc attaggaaac cgtgtagtca tcgaaaagaa
    14881 agaacatgaa caaacaacga aaagcggtat tgttttaact gattcagcta aggaaaaatc
    14941 caatgaaggt gttatcgttg cggtaggtcc gggacgcact ttagacaatg gtgaacgcct
    15001 aaaaccagaa ctcaacgtag gcgatcgtgt tgtgtttcaa caatatgcag gaactgaagt
    15061 gaagcgtgac gacacagaat acttagtgtt aactgaagat gaagtgttag ctgtgattga
    15121 agactaaatt taaactgata tataaaagaa atgaatgaaa caacggaggt aatagaatgg
    15181 caaaggaatt aaaattttct gaagatgcac gtcaagcgat gttacgtggt gtcgataaat
    15241 tagcaaatgc tgttaaagtg acaattggtc caaaagggcg caatgtcgta ctagataaag
    15301 aatttacagc accactcatt acaaatgatg gtgtaacgat tgcaaaagaa attgaattag
    15361 aagatcctta tgaaaatatg ggtgcgaaac ttgttcaaga agttgcgaat aaaacgaatg
    15421 aaattgcagg agacggtacg acaacagcga ctgtacttgc gcaagcgatg attcaagaag
    15481 gacttaaaaa cgtaacaagt ggtgcgaacc cagttggaat ccgtcaaggt attgataaag
    15541 cagtggcggt cgcaattgaa tcattacaca acatttcgca aaaagttgaa aacaaagaag
    15601 aaattgcaca agtcggcgcg atttctgcag ctgacgaaga agtaggtcgt tacatttctg
    15661 aagcgatgga aaaagtcggt aatgacggtg tgatctcaat tgaagaatca aatggtttta
    15721 acacagaatt agaagtcgtt gaagggatgc aatttgatcg tggttaccaa tcgccataca
    15781 tggtgacaga ttctgacaaa atgattgcag aattagaaag accatacatt ttaattactg
    15841 acaagaaaat ctcttcattc caagatatct tgccattatt agagcaaatt gtacaatcca
    15901 accgtcctat tttaattgta gcggacgaag tagaaggcga tgcgttaacc aaccttgtct
    15961 taaaccgtat gcgtggtaca tttacagccg ttgcagttaa ggcacctggt ttcggtgatc
    16021 gtcgtaaagc aatgttagaa gacttggcga ttgtaacagg cgcacaagtg attacagacg
    16081 atttaggtct tgaattaaaa gaagcgactt tagatatgtt aggtacggca aacaaagttg
    16141 aagtgactaa agacaataca acagtggttg atggtgacgg tgatcctgcg aacatcgatg
    16201 cacgtgtcgg tcaattaaaa gcacaaattg aagaaactga ttctgacttt gatcgcgaaa
    16261 aacttcaaga gcgcttagca aaattagctg gcggtgttgc ggtgattaaa gtgggtgcag
    16321 cgtctgaaac ggaattgaaa gaacgtaaat tacgtattga agatgcatta aactcaacac
    16381 gtgcagcagt agaagaaggt attgttgcag gtggaggtac ggcattaatg aacattttca
    16441 aagacgtcca agctatcgaa gcggaaggcg acgaagcaac aggtgtgaac atcgtattga
    16501 aagcattgca agcacctgta cgtcaaattg ctgaaaatgc aggtcttgaa ggttcagtca
    16561 tcgttgaaag aatgaaaaat gctgaaccag gtattggtta caacgcagct acagacgaat
    16621 gggtgaacat gttagaagca ggtatcgtgg acccaacgaa agtgacacgt tctgctttac
    16681 aacacgcagc aagtgttgcg gcaatgttct taacaactga agcggtagtt gcaaacatcc
    16741 ctgaagataa agggaatgat atgccacaaa tgggcggcat gccagggatg atgtaaaaca
    16801 gcttataaat gatgatttaa tggcatttta taattgtgaa tgacatatta ataacataaa
    16861 agatttcaaa aagataaaaa tgccgtttaa ttcagatgaa ccaaacgaac atttaaattc
    16921 attcttttga ggcgttttcc ttaagcgagc aaaacttatg ggaagcgtcc tttttgtgtg
    16981 aattggtaat ctcagcataa atctttaaag acgtattttt aaaaaactgt attaggtaag
    17041 cggcgcataa atagggtgat tgataaaaat gagtaatatt aatagttgca tggaagtaag
    17101 tggtttaaaa ttctctgaag gtgaaaatgc tgttcattta gatttaagtg aaggcgaata
    17161 ccctttttta gatgatggaa aaagctttaa caaagattat tttgaaagga ttcataacaa
    17221 tgcaattcaa attttacaag atttgtttca acaaagtaat actattgata tagttctttt
    17281 atattattta tatggggact catttaaaaa aactaggttc aaagaaaagt ttagttattt
    17341 caataataac gaaataccag attttaaaga atatattaat gatgaaggca tccaatgtta
    17401 tattttttct tataaaaata agacattgaa ggatttaaat tataaaaaat tagtgcgagc
    17461 aatttccaat caagatttta agggtttatt cccaacaatt aatcaaaaag acaattactt
    17521 agatatatat ttaatggata gtaaaagagg catattattt cacttgtatg atgatagagg
    17581 gctgtggcta tattttctaa ataagcagtc atataaaatt tattctcaaa aatacagcaa
    17641 cctcttgttt gatgtaagtc atgaaatgga ttaaagttta tttaaaatta ataatactaa
    17701 ttttattatc ggtatttata tatttgaatt ataattgcaa gtatgaaaaa agtaaaatag
    17761 cagttctaga tacaggtgtt gaaaagaaag ctattaaaag caaagtaata tctcgtaatt
    17821 ttactcttga caataactgg aatagcgtac atgctaataa agtttcaaaa attatagaat
    17881 atgaagcaga tatagttatt tacgatgcta aagttttgaa ccgtcatggt aacggaagat
    17941 tggaggatac tatttcagca cttgactggg caattaaaaa taatgtcgat ataataaata
    18001 tgagttatgg ctttactaaa aattatcctg aattacatag aaaaataaaa gaagctcatg
    18061 aaaaaggcat tattattgta gctgcaaacg gtaacgacat ttttggtggt aaagaatttc
    18121 cagcatccta taaagaaact atatcggttg gagtcttgaa taaggaaggt tcaaagtctg
    18181 tctttaattc taacgatgat gctgacgtat tcatttcggt tgataataag ttgcctaata
    18241 gcggtgagaa tacttctatg gctacagctt atgtatctaa taaattgact aaactgtgca
    18301 ataggaactt gagaaacatg gacaaagatg aaatattaaa gttaattaat gagttagatt
    18361 aattaagaat gtataataat tatgtttgca caaaagttaa aaatacgaat caatttttaa
    18421 catttatgag gcaattaggt tattaagttt gagatatgtg atgctagaat gacatcatac
    18481 tcgttatgat atagcctcat cacacttcat aacaaaataa aaaccacttt ccaatatggt
    18541 gtcatgacat ttggaaagtg gtttttttac gtattatgaa aatgtgtaac attaccgcac
    18601 aggatgctca taccattctt ttaaaaaggc gtatagcact tctgtaaaat gatccaaatc
    18661 ttgaatgcga attttttcat tcgttccatg ggcttcattg agctctcctg gaccgtataa
    18721 aatagttgga atgccaaagt ctgcggtcca accaccatct gtcacagttg tgctcattgt
    18781 cgtttgaagc ggtgtctggt gtacgtgttc atgtgcttta gctaacatat gaaagcctgc
    18841 gtgtgttgtt ggtaaagtaa aactagggaa gatttcacct ttatcctcaa tcatagaagt
    18901 gccaccccat tcgaattgaa gtgggttttc attgagccat aaatcactct cagccacttt
    18961 atttaagtac gtttctattt cttcaataat cgtttcgtaa ttttcatcag gtaaataatg
    19021 cactgttatc cataaacgac attgatcggc aataaaagca gggtgacgtc cgccttcaat
    19081 gactgcagga ttaatcgtgt tagcaccggg aggcatacct ggacaagatt tagtgactgc
    19141 ccaatgacgt tctagttctt gaagtgcttg aatgattttt accatttttt caatcgcact
    19201 ggcaccgtga cgtccgccgc cagcatgaat tatatgactt cgtgcaccgt cgtgaatcgt
    19261 ttcttggctt tggatggtca cccaacctgt aatgacgcca ccttgaccca tcgcaatatt
    19321 gtcactcgta tctaacacaa gtgctaaatc agcttttggt gaatgttcac aagcgacttt
    19381 tgtacctgct tcaccgactt cttcgccgac aacagaatgt acgataatat cgccttcagg
    19441 ttgtaagcct tcttgatgca agcgttctaa attataaaag agggcggaca tgccaccttt
    19501 catatcagca acgcctctac caaatagaaa accatctact tcagtcagtt gaaaaggatt
    19561 gaagcgccaa aattgttcat cttctacttc tgcaacatcg acatggccat ttaaaatcag
    19621 gcgaggggca tcggggtttc gacctttcaa tgtaccaaca ataattgtgt cattatcata
    19681 gaaaggaatg gtttgtacgt caaaacccgt attttcgagc caagttgaaa tttgttgctg
    19741 taaagtctgc gtgttacggg ctgggggact cactgtcgga tgcgcaatta atgtttgaag
    19801 tatatcaaat tgtcgttgtt ccataaggat gaccctccat tgctaataaa tattttttac
    19861 gatgaagtcc gctattgaat ccacctaatt gcccatcttt tcgtaagaca cgatgacacg
    19921 gtacgataat agacaatgga ttttgaccga tagcagatgc cacagcacgt acactttttg
    19981 gacggccaat catttgtgca atatcactat aatttacaac cgccccaaaa gggagttgtt
    20041 gcaacgcttg ccacacttgt tgttgaaatg gcgtaccaat gacgtgcgcg acgggtgttt
    20101 tgaatgtgtg ataatggcca tgaaaataag cttctaactc taaagcaatt tggttcacaa
    20161 tcggatgatg tgaaatttga acgagtggtg cttgcttttg aaaatgtttc aatgcagtat
    20221 gataatcttt tgagtctgta aatgacaaac ctaacaaggc gttatcattc acaacagcgg
    20281 tcattaaccc aagtggtgta ttaatatgtt gaaagtaaaa tgacatccga ttcgacctcc
    20341 tttaatttga ttgaaatggc acgaaaccct cacgactgat cgcaatgttg tcatttggaa
    20401 aaacgtcttg cattaatttt atcacatgca gataggcgtc tggatgatac caagactgta
    20461 aatcgagatt ttcgtataaa gattgaatgc cgagtgagtg ggtgcgacga ccgtttgacc
    20521 cgtcacccat aaagtaatcg tcaatcgtaa catggtcgac atgttcagct aatcgttcgg
    20581 caaactgatc cgtacaaggt agcacaggtg caatagcaat ttgtgtgggg atttgagctg
    20641 cgcgaagttt gtccacagtt ttcaatcttg cttgaatgct cggtgctttt ggtgtgaaat
    20701 ggcgaa
//
