GCF_900607225.1 - 12 region(s)

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Location: 208,033 - 276,516 nt. (total: 68,484 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

E0E39_RS01075: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: glycosyltransferase

E0E39_RS01055: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

Hydrolases: esterase

E0E39_RS01025: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

E0E39_RS01015: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

E0E39_RS01020: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

E0E39_RS01030: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

E0E39_RS01035: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

E0E39_RS01040: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

E0E39_RS01045: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.90	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.90	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.89	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001160.5	0.62	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001132.4	0.62	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002192.3	0.62	PKS	FR901512	fungal sp. No.14919

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001164.3	0.67	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.66	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001641.4	0.66	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.66	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.66	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 25.19 out of 30.22

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.37 out of 20.47

Confidence: medium

Additional weak hits (5):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.42 out of 26.85

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.18 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.13 out of 32.74

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 23.67 out of 30.12

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.21 out of 23.43

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 208032 - 276516:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ohmal) + (Me-redmal) + (Me-ccmal) + (mal) + (NMe-Pro)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 208032 - 253108:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ohmal) + (Me-redmal) + (Me-ccmal) + (mal) + (NMe-Pro)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 213156 - 276516:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ohmal) + (Me-redmal) + (Me-ccmal) + (mal) + (NMe-Pro)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

E0E39_RS01015: Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (469..855): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: VSECFDVFILEAIYSTIGEFNLYGPAEASIVTLG
Nearest Stachelhaus code(s):: Leu DVLIFGLIYK (53% 8Å match)
Stachelhaus code match:: 70% (weak)

E0E39_RS01020: mal

PKS_AT (534..816): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 88.9
inactive: 58.3
Methoxymalonyl-CoA: 45.2
Methylmalonyl-CoA: 25.2
Propionyl-CoA: 24.3
2-Methylbutyryl-CoA: 9.9
Acetyl-CoA: 9.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

E0E39_RS01030: mmal

PKS_AT (457..737): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 75.0%; Malonyl-CoA: 66.7%; Ethylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 107.7
Methoxymalonyl-CoA: 82.8
Malonyl-CoA: 77.3
Ethylmalonyl-CoA: 67.5
Isobutyryl-CoA: 46.7
Propionyl-CoA: 46.6
fatty_acid: 44.3
2-Methylbutyryl-CoA: 40.0
Acetyl-CoA: 39.1
trans-1,2-CPDA: 36.0
Benzoyl-CoA: 35.2
CHC-CoA: 19.2
inactive: 17.7
3-Methylbutyryl-CoA: 15.5

E0E39_RS01035: mmal

PKS_AT (1479..1766): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Methoxymalonyl-CoA: 79.2%; Ethylmalonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 134.2
Ethylmalonyl-CoA: 108.8
Methoxymalonyl-CoA: 104.6
Malonyl-CoA: 95.9
trans-1,2-CPDA: 78.7
2-Methylbutyryl-CoA: 71.6
Propionyl-CoA: 69.1
Isobutyryl-CoA: 69.1
Acetyl-CoA: 50.9
Benzoyl-CoA: 42.9
CHC-CoA: 38.3
fatty_acid: 31.6
3-Methylbutyryl-CoA: 28.5
inactive: 22.6

E0E39_RS01040: mmal

PKS_AT (525..802): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 79.2%; Malonyl-CoA: 70.8%; Methoxymalonyl-CoA: 70.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 110.7
Malonyl-CoA: 91.8
Methoxymalonyl-CoA: 89.3
Ethylmalonyl-CoA: 65.4
Propionyl-CoA: 59.1
2-Methylbutyryl-CoA: 52.9
Benzoyl-CoA: 46.9
inactive: 43.0
Isobutyryl-CoA: 41.0
fatty_acid: 38.8
Acetyl-CoA: 37.0
trans-1,2-CPDA: 34.9
CHC-CoA: 26.2
3-Methylbutyryl-CoA: 22.6

E0E39_RS01045: X

AMP-binding (31..454): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: NQSLHDMGL--ATGLNGSERPSFGMAEATVFVSC
Nearest Stachelhaus code(s):: OMe-Trp DAFTLGCVFK (29% 8Å match)
Stachelhaus code match:: 70% (weak)

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
ClgR	Caseinolytic protease gene regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_LR027842.1 - Region 2 - lanthipeptide-class-ii

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Location: 343,542 - 366,532 nt. (total: 22,991 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase

E0E39_RS01500: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000554.4	0.69	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0002005.2	0.51	ribosomal	RaxX	Xanthomonas oryzae pv. oryzae
BGC0001579.4	0.51	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0002698.2	0.50	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001210.5	0.49	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0001226.4	0.49	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0000563.4	0.48	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0000528.5	0.48	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000503.5	0.46	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002699.2	0.46	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000554.4	0.69	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0002005.2	0.51	ribosomal	RaxX	Xanthomonas oryzae pv. oryzae
BGC0001579.4	0.51	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0002698.2	0.50	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001210.5	0.49	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0001226.4	0.49	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0000563.4	0.48	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0000528.5	0.48	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000503.5	0.46	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002699.2	0.46	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for E0E39_RS01500

E0E39_RS01490

Leader:

MNSSDSAILLHNPAGIVELDTDDMDLDMTHGG

Core:

TTWWCAIATATIALTATKCSPDGTLCGSCQMGTRGCC

Core with dehydrations:

DhbDhbWWCysAIADhbADhbIALDhbADhbKCysDhaPDGDhbLCysGDhaCysQMGDhbRGCysCys

antiSMASH-DB 4.0 matches for E0E39_RS01490

100.0%

Lanthipeptides: E0E39_RS01490_lanthipeptide

1

TTWWCAIATATIALTATKCSPDGTLCGSCQMGTRGCC

37

TTWWCAIATATIALTATKCSPDGTLCGSCQMGTRGCC

1

TTWWCAIATATIALTATKCSPDGTLCGSCQMGTRGCC

37

78.4%

NZ_FNAD01000024.1:

Lanthipeptides: BLQ63_RS26045_lanthipeptide

1

TTWWCAIATATIALTATKCSPDGTLCGSCQMGTRGCC

37

+TW C TATIALT T CSP GTLCGSCQMGTRGCC

1

STWGCV--TATIALTVTVCSPTGTLCGSCQMGTRGCC

35

73.0%

NZ_BMTW01000001.1and 1 other:

NZ_KZ268499.1 Lanthipeptides: CE422_RS19980_lanthipeptide

Lanthipeptides: IE134_RS03420_lanthipeptide

1

TTWWCAIATATIALTATKCSPDGTLCGSCQMGTRGCC

37

TTW CA TI LT T CSP GTLCGSC MGTRGCC

1

TTWACA----TITLTVTVCSPTGTLCGSCSMGTRGCC

33

with 12 more sequence matches

Only the first 10 are displayed here.

70.3%

NZ_DS999644.1and 2 others:

NZ_LACR01000025.1 Lanthipeptides: YW5_RS27795_lanthipeptideNZ_ML123034.1 Lanthipeptides: EES43_RS16615_lanthipeptide

Lanthipeptides: SSGG_RS13165_lanthipeptide

1

TTWWCAIATATIALTATKCSPDGTLCGSCQMGTRGCC

37

TTW CA T+ LT T CSP GTLCGSC MGTRGCC

1

TTWACA----TVTLTVTVCSPTGTLCGSCSMGTRGCC

33

59.5%

NZ_CP070496.1:

Lanthipeptides: JQS30_RS16095_lanthipeptide

1

TTWWCAIATATIALTATKCSPDGTLCGSCQMGTRGCC

37

TTW CA T+ LT T CSP T+CGSC GT GCC

1

TTWGCA----TVTLTVTVCSPTNTMCGSCSWGTSGCC

33

47.4%

NZ_CP013979.1:

Lanthipeptides: allorf_0343557_0343787_lanthipeptide

1

TTWWCAIATATIALTATKCSPDGTLCGSCQMGTRGCC

37

T W CA + + ++ CSP GT CGSC GT GCC

1

TGWACATVSIGVGISLAWCSPGGTFCGSCTFGTSGCC

37

40.5%

NZ_CP014228.1:

Lanthipeptides: AXF14_RS11840_lanthipeptide

10

ATIALTATKCSPDGTLCGSCQMGTRGCC

37

A + T T C D L GSC++GTRGCC

7

AFVVTTVTACWNDTALWGSCRLGTRGCC

34

35.1%

NZ_CP063989.1:

Lanthipeptides: ID810_RS00800_lanthipeptide

19

CSPDGTLCGSCQMGTRGCC

37

C D L GSC+MGTRGCC

16

CFSDTVLWGSCRMGTRGCC

34

35.1%

NZ_GL405260.1:

Lanthipeptides: allorf_2499753_2499935_lanthipeptide

12

IALTATKCSPDGTLCGSCQMGTRGCC

37

I T T+C D + GSC++GTR CC

9

IIATLTQCFNDTLVWGSCRLGTRACC

34

24.3%

NZ_JABBGE010000004.1and 1 other:

NZ_JABBGE010000004.1 Lanthipeptides: HHL19_RS13570_lanthipeptide

Lanthipeptides: HHL19_RS13570_lanthipeptide

1

TTWWCAIATATIALTATKCSP

21

TTW CA+ TA L + P

1

TTWSCAVVTAVATLVSASACP

21

21.7%

NZ_AKXV01000031.1and 3 others:

NZ_AKXV01000031.1 Lanthipeptides: Y19_RS44045_lanthipeptideNZ_LMEI01000006.1 Lanthipeptides: ASC82_RS30550_lanthipeptideNZ_LMEI01000006.1 Lanthipeptides: ASC82_RS30550_lanthipeptide

Lanthipeptides: Y19_RS44045_lanthipeptide

1

TTWWCAIATATIALTATKCSP

21

TTW CAI TA L + P

18

TTWSCAIVTAVATLVSASACP

38

18.9%

NZ_CP011114.1:

Lanthipeptides: VK70_RS27255_lanthipeptide

19

CSPDGTLCGSCQMG

32

C+P TLC SC

11

CNPTATLCTSCAFN

24

18.9%

NZ_CP013023.1:

Lanthipeptides: allorf_0505345_0505512_lanthipeptide

19

CSPDGTLCGSC

29

C+P TLC SC

11

CNPTATLCTSC

21

E0E39_RS01495

Leader:

MRNDDVNMNSSEGLLAGVDHPSGLIDLTELSDIELDETSGA

Core:

AGTGLVGTFGCCWCVPWYSGWTVCGLVCSHPGCKR

Core with dehydrations:

AGDhbGLVGDhbFGCysCysWCysVPWYDhaGWDhbVCysGLVCysDhaHPGCysKR

antiSMASH-DB 4.0 matches for E0E39_RS01495

100.0%

BGC0001155.5 (AGY49587.1):

Lanthipeptides: E0E39_RS01495_lanthipeptide

1

AGTGLVGTFGCCWCVPWYSGWTVCGLVCSHPGCKR

35

AGTGLVGTFGCCWCVPWYSGWTVCGLVCSHPGCKR

1

AGTGLVGTFGCCWCVPWYSGWTVCGLVCSHPGCKR

35

65.7%

NZ_FNAD01000024.1:

Lanthipeptides: BLQ63_RS26050_lanthipeptide

1

AGTGLVGTFGCCWCVPWYSGWTVCGLVCS

29

AGT +GTFGCCWC+PWYSGWTVCG +CS

1

AGTQHMGTFGCCWCLPWYSGWTVCGSLCS

29

62.9%

NZ_CP014228.1:

Lanthipeptides: AXF14_RS11835_lanthipeptide

1

AGTGLVGTFGCCWCVPWYSGWTVCGLVCSHPGCK

34

AG+G + TFGCCWCVPWYS +T CGL CS C+

1

AGSGWMYTFGCCWCVPWYSSFTKCGLACSQGTCR

34

with 6 more sequence matches

60.0%

NZ_CP013979.1:

Lanthipeptides: ATC03_RS01475_lanthipeptide

2

GTGLVGTFGCCWCVPWYSGWTVCGLVC

28

GT +GTFGCCWCVPW+SG+TVCG VC

1

GTNPLGTFGCCWCVPWWSGFTVCGAVC

27

60.0%

NZ_CP063989.1:

Lanthipeptides: ID810_RS00795_lanthipeptide

1

AGTGLVGTFGCCWCVPWYSGWTVCGLVCSHPGCK

34

AG+G V TFGCCWC+PWYS T CG +CS C+

1

AGSGWVYTFGCCWCLPWYSSLTKCGAICSQGTCQ

34

57.1%

NZ_DS999644.1and 1 other:

NZ_LACR01000025.1 Lanthipeptides: YW5_RS27800_lanthipeptide

Lanthipeptides: SSGG_RS13160_lanthipeptide

2

GTGLVGTFGCCWCVPWYSGWTVCGLVCS

29

G+G++GT GCC C+PWYSGWTVCGL C+

1

GSGVLGTLGCCSCLPWYSGWTVCGLACN

28

54.3%

NZ_GL405260.1:

Lanthipeptides: HMPREF0580_RS12195_lanthipeptide

2

GTGLVGTFGCCWCVPWYSGWTVCGLVCSHPGC

33

G+ T GCCWC+PWYS WT CG+VCS C

1

GSAAFKTLGCCWCLPWYSSWTKCGVVCSEQYC

32

28.6%

NC_015711.1:

Lanthipeptides: allorf_5704565_5704741_lanthipeptide

14

CVPWYSGWTVCGLVCSHPGC

33

CV W S LV S+PGC

8

CVRWLSRNCCTSLVSSNPGC

27

20.0%

NZ_CP020567.1:

Lanthipeptides: B6264_RS23020_lanthipeptide

19

SGWTVCGLVCSH

30

GW+ CG VCS+

10

DGWSSCGRVCSY

21

MIBiG 4.0 matches for E0E39_RS01495

14.3%

Thiopeptide: GE2270

23

VCGLVCS

29

VCG CS

5

VCGFCCS

11

11.4%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

31

PGCK

34

PGCK

9

PGCK

12

11.4%

BGC0001155.5 (AGY49587.1):

Thiopeptide: GE2270

11

CCWCVP

16

CC C P

9

CCSCSP

14

allorf_0352113_0352232

Leader:

MAKHGGDMDPAGS

Core:

SRQHTARKQASITNFQAEPLRVHLCI

Core with dehydrations:

DhaRQHDhbARKQADhaIDhbNFQAEPLRVHLCysI

antiSMASH-DB 4.0 matches for allorf_0352113_0352232

100.0%

Lanthipeptides: allorf_0352113_0352232_lanthipeptide

1

SRQHTARKQASITNFQAEPLRVHLCI

26

SRQHTARKQASITNFQAEPLRVHLCI

1

SRQHTARKQASITNFQAEPLRVHLCI

26

28.1%

NZ_JAMTCO010000001.1:

Lanthipeptides: allorf_474390_474536_lanthipeptide

1

SRQHTARKQASITNFQAE

18

SR HT R A QAE

14

SRTHTGRDHACSPICQAE

31

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (5):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.34 out of 28.14

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 24.03 out of 30.12

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.57 out of 36.53

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.35 out of 27.55

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.47 out of 28.19

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

E0E39_RS01490 - Class II

Cleavage pHMM score: -2.40

RODEO score: 0

Monoisotopic mass: 3601.7 Da

Molecular weight: 3604.2 Da

Number of bridges: 6

Alternative weights: (assuming N unmodified Ser/Thr residues); 3622.2 Da N = 1; 3640.2 Da N = 2; 3658.2 Da N = 3; 3676.3 Da N = 4

E0E39_RS01495 - Class II

Cleavage pHMM score: -0.20

RODEO score: 0

Monoisotopic mass: 3599.6 Da

Molecular weight: 3602.2 Da

Number of bridges: 5

allorf_0352113_0352232 - Class II

Cleavage pHMM score: -5.30

RODEO score: 0

Monoisotopic mass: 2931.6 Da

Molecular weight: 2933.3 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2951.4 Da N = 1; 2969.4 Da N = 2; 2987.4 Da N = 3

TFBS definitions

Regulator	Description

NZ_LR027842.1 - Region 3 - NRPS

Download region SVG

Location: 493,912 - 560,009 nt. (total: 66,098 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

E0E39_RS02120: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

E0E39_RS02250: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E0E39_RS02285: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Hydrolases: esterase hydrolase

E0E39_RS02170: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

E0E39_RS02215: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

E0E39_RS02135: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase

E0E39_RS02200: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

E0E39_RS02205: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

E0E39_RS02210: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Translocases: transport

E0E39_RS02225: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001758.4	0.75	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002071.2	0.72	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001132.4	0.72	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001758.4	0.75	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002071.2	0.72	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001132.4	0.72	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.46 out of 20.47

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.86 out of 18.57

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 16.35 out of 18.57

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.78 out of 25.88

Confidence: medium

Additional weak hits (18):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.58 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.98 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.67 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.85 out of 18.57

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.22 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.47 out of 27.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.58 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.54 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.56 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 19.31 out of 28.14

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 19.26 out of 31.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.12 out of 27.78

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.76 out of 20.97

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.87 out of 24.78

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 21.38 out of 27.18

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.05 out of 35.63

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 24.05 out of 35.63

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.57 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 5, location 493911 - 560009:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - Leu) + (X - X - Leu - X) + (Asn)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

E0E39_RS02200: X - X - Leu

Search NORINE for peptide: strict or relaxed

AMP-binding (9..391): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LSQAFDASVKDGLVFVGAEVNTYGPTEATVTCLH
Nearest Stachelhaus code(s):: dh-Trp DAALMGAVTK (50% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1049..1439): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: MSEAFDASVFEMFMVLAGEVNLYGPTEATIFASG
Nearest Stachelhaus code(s):: Val DAFFVGGTFK (50% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (2088..2466): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAQAFDASILELLVLVGAEFNAYGPTETTVAATV
Nearest Stachelhaus code(s):: 3OH-Leu DALLVGAVAK (74% 8Å match); Leu DALLIGAVAK (71% 8Å match)
Stachelhaus code match:: 90% (moderate)

E0E39_RS02205: X - X - Leu - X

Search NORINE for peptide: strict or relaxed

AMP-binding (462..845): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TSQAFDVSVIDTELNLSGELNTYGPTEVTVTSVV
Nearest Stachelhaus code(s):: Orn DVMELSSITK (56% 8Å match)
Stachelhaus code match:: 60% (weak)

AMP-binding (1491..1877): X

nrpys: Ile, Glu

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LGYAFDGAVSEMFLLVGGEFNVYGPTETTVDISY
Nearest Stachelhaus code(s):: Glu DGWKLGVVDK (62% 8Å match); Ile DGFFLGVVYK (53% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (2535..2913): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FSLAFDAAIMEMLLVAGAEFNAYGPTEATIAVAV
Nearest Stachelhaus code(s):: Leu DAMLVGAICK (59% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (3587..3982): X

nrpys: Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LSQAFDASVSEMIFMVAGELNLYGPTETTIDVTR
Nearest Stachelhaus code(s):: bOH-Tyr DASTIAAVDK (71% 8Å match); Cl-Tyr or Tyr DASTVAAIGK (65% 8Å match); Gln DAWQFGLIDK (62% 8Å match); Ser DVWHMSLIDK (53% 8Å match)
Stachelhaus code match:: 60% (weak)

E0E39_RS02210: Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (522..908): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YDVQFDFSLPMLKMVLSGEINNYGPTEAIGACTT
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

TFBS definitions

Regulator	Description
PsrA	Oleic acid responsive regulator
ToxT	Regulator of MSHA biosynthesis
VqsM	Regulator of quorum-sensing signalling systems

NZ_LR027842.1 - Region 4 - NRPS,T1PKS

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Location: 669,786 - 725,712 nt. (total: 55,927 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: PPTase acylase aminotransferase

E0E39_RS02820: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

E0E39_RS02865: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

E0E39_RS02880: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

E0E39_RS02855: thioesterase

smcogs: SMCOG1004: thioesterase

E0E39_RS02900: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase

smcogs: SMCOG1283: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase

Lyases: hydratase

E0E39_RS02895: Nitrilase/cyanide hydratase and apolipoprotein

smcogs: SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein

Ligases: synthase

E0E39_RS02825: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

E0E39_RS02875: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001132.4	0.65	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001244.3	0.60	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.60	PKS	asperlactone	Aspergillus ochraceus
BGC0001641.4	0.60	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001164.3	0.60	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.58	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000037.4	0.53	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0002191.2	0.53	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002158.2	0.39	NRPS, PKS	tenuazonic acid	Pyricularia oryzae 70-15
BGC0002058.3	0.35	NRPS, PKS	mutanocyclin	Streptococcus mutans B04Sm5

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001132.4	0.66	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001244.3	0.64	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.64	PKS	asperlactone	Aspergillus ochraceus
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000037.4	0.60	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0002191.2	0.60	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002158.2	0.52	NRPS, PKS	tenuazonic acid	Pyricularia oryzae 70-15
BGC0002058.3	0.49	NRPS, PKS	mutanocyclin	Streptococcus mutans B04Sm5

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.80 out of 26.33

Confidence: medium

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.93 out of 26.33

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 22.51 out of 25.88

Confidence: medium

Additional weak hits (7):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.77 out of 27.78

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.62 out of 26.12

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.85 out of 18.57

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.67 out of 32.74

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.76 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.56 out of 29.38

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.23 out of 30.98

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 6, location 669785 - 725712:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - mal - X - Leu) + (mal)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

E0E39_RS02880: X - mal - X - Leu

Search NORINE for peptide: strict or relaxed

AMP-binding (463..862): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TDLTFDIFGTEGNSFSGGEENVYGPTEATMWATH
Nearest Stachelhaus code(s):: Gly DILQFGVIWK (59% 8Å match)
Stachelhaus code match:: 70% (weak)

PKS_AT (1569..1863): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 120.4
inactive: 69.6
Methoxymalonyl-CoA: 37.4
Methylmalonyl-CoA: 28.8
Propionyl-CoA: 27.1
Isobutyryl-CoA: 15.4
2-Methylbutyryl-CoA: 8.1
CHC-CoA: 5.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

AMP-binding (2469..2859): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LDHAFDIFAFEIQVVCGGEFNMYGPTETTVTTTV
Nearest Stachelhaus code(s):: Gly DILQLGMVWK (62% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (4321..4704): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: ADTTFDPSMFDSLLFVGGEFNLYGPTESSIVSTF
Nearest Stachelhaus code(s):: Htia DPHHFGLIGK (50% 8Å match)
Stachelhaus code match:: 70% (weak)

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
PsrA	Oleic acid responsive regulator

NZ_LR027842.1 - Region 5 - NRPS,lanthipeptide-class-iii

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Location: 746,601 - 810,205 nt. (total: 63,605 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acetyltransferase

E0E39_RS03315: Chloramphenicol acetyltransferase

smcogs: SMCOG1156: Chloramphenicol acetyltransferase

Hydrolases: back translocase deaminase

E0E39_RS03180: GTP-binding protein LepA

smcogs: SMCOG1190: GTP-binding protein LepA

E0E39_RS03200: GTP-binding protein LepA

smcogs: SMCOG1190: GTP-binding protein LepA

E0E39_RS03245: ornithine cyclodeaminase

smcogs: SMCOG1158: ornithine cyclodeaminase

Ligases: ligase synthase

E0E39_RS03215: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

E0E39_RS03225: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

E0E39_RS03240: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001825.4	0.39	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0000513.3	0.37	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0001261.3	0.37	NRPS	AM-toxin	Alternaria alternata
BGC0001833.4	0.37	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.36	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001240.4	0.35	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0000357.5	0.35	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0002286.3	0.35	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.35	NRPS	HC-toxin	Alternaria jesenskae
BGC0000342.4	0.34	NRPS	enniatin	Fusarium equiseti

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001825.4	0.71	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001833.4	0.69	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.69	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001240.4	0.68	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0000357.5	0.68	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0002286.3	0.68	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.68	NRPS	HC-toxin	Alternaria jesenskae
BGC0000342.4	0.67	NRPS	enniatin	Fusarium equiseti
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 17.75 out of 18.57

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 17.75 out of 18.57

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.34 out of 27.78

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 20.77 out of 28.19

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.28 out of 19.52

Confidence: medium

Additional weak hits (15):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.00 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.34 out of 26.06

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 19.33 out of 30.97

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.58 out of 32.74

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.84 out of 32.74

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 23.07 out of 30.22

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.76 out of 30.25

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.64 out of 27.55

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.00 out of 30.98

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.73 out of 30.98

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.00 out of 28.19

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.79 out of 22.40

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.34 out of 27.18

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.04 out of 20.01

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.46 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Core peptide predictions

No core peptides found.

Predicted core structure(s)

For candidate cluster 7, location 746600 - 810205:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - D-X) + (X - D-X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 9, location 754047 - 810205:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - D-X) + (X - D-X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

E0E39_RS03215: X - X

AMP-binding (439..832): X

nrpys: Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ALLSFDLALCEISAMTVGETNGYGPTETTICASI
Nearest Stachelhaus code(s):: Cys DHESDVGITK (50% 8Å match)
Stachelhaus code match:: 60% (weak)

AMP-binding (1485..1894): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HAKAFDVSIWQTEVLSTGEVNAYGPTECSDDITH
Nearest Stachelhaus code(s):: Dpr DIWELTADDK (88% 8Å match); Ser DVWEMTADDK (82% 8Å match); Dab DIWELTADDK (68% 8Å match)
Stachelhaus code match:: 90% (moderate)

E0E39_RS03225: X - X

AMP-binding (448..853): X

nrpys: Dpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HAKAFDISIWQLELMSTGEVNAYGPTECSDDVTH
Nearest Stachelhaus code(s):: Dpr DIWEMTADDK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1484..1867): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FNIGFDAIIWEVLVFVGGERNVYGPTETTVVCLV
Nearest Stachelhaus code(s):: Gln DAWQFGVVDK (53% 8Å match)
Stachelhaus code match:: 80% (moderate)

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
ToxT	Regulator of MSHA biosynthesis
ZuR variant 1	Zinc-responsive repressor

NZ_LR027842.1 - Region 6 - terpene-precursor

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Location: 968,149 - 989,231 nt. (total: 21,083 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

E0E39_RS04265: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Hydrolases: esterase

E0E39_RS04215: enterobactin/ferric enterobactin esterase

smcogs: SMCOG1098: enterobactin/ferric enterobactin esterase

Ligases: synthetase

E0E39_RS04225: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001870.3	0.45	other (other)	putrebactin, avaroferrin	Xenorhabdus budapestensis
BGC0002073.3	0.45	NRPS	dehydroxynocardamine	Corynebacterium propinquum
BGC0002494.3	0.44	NRPS	vibriobactin	Vibrio cholerae
BGC0000947.5	0.43	other (other)	vibrioferrin	Vibrio alginolyticus
BGC0002150.2	0.43	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.43	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0002476.2	0.42	NRPS	enterobactin	Escherichia coli str. K-12 substr. MG1655
BGC0002682.2	0.42	other (other)	aerobactin	Vibrio mimicus
BGC0000410.5	0.42	NRPS	pseudomonine	Pseudomonas fluorescens

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001870.3	0.45	other (other)	putrebactin, avaroferrin	Xenorhabdus budapestensis
BGC0002073.3	0.45	NRPS	dehydroxynocardamine	Corynebacterium propinquum
BGC0002494.3	0.44	NRPS	vibriobactin	Vibrio cholerae
BGC0000947.5	0.43	other (other)	vibrioferrin	Vibrio alginolyticus
BGC0002150.2	0.43	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.43	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0002476.2	0.42	NRPS	enterobactin	Escherichia coli str. K-12 substr. MG1655
BGC0002682.2	0.42	other (other)	aerobactin	Vibrio mimicus
BGC0000410.5	0.42	NRPS	pseudomonine	Pseudomonas fluorescens

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

E0E39_RS04225: PT_FPPS_like

Domain 1 (47 - 348): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (5):

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.08 out of 20.97

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.20 out of 21.55

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 24.84 out of 36.53

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.08 out of 33.14

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.15 out of 27.18

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description

NZ_LR027842.1 - Region 7 - azole-containing-RiPP

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Location: 2,419,558 - 2,447,706 nt. (total: 28,149 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

E0E39_RS10845: amidohydrolase

smcogs: SMCOG1244: amidohydrolase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002115.3	0.50	ribosomal	streptamidine	Streptomyces albidoflavus J1074
BGC0000590.5	0.50	ribosomal	microcin N	Escherichia coli
BGC0002698.2	0.49	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0002144.3	0.47	ribosomal	phazolicin	Rhizobium sp. Pop5
BGC0000483.5	0.47	ribosomal	aeruginosamide B, aeruginosamide C	Microcystis aeruginosa PCC 9432
BGC0002551.2	0.47	other (other), ribosomal, terpene	muscoride A, muscoride B	Nostoc sp. UHCC-0398
BGC0002485.2	0.46	other (other), ribosomal	muscoride A, muscoride B	Desmonostoc sp. PCC 7906
BGC0002306.2	0.45	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0000475.5	0.43	ribosomal	patellamide A, patellamide C	Prochloron didemni
BGC0002699.2	0.43	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002115.3	0.50	ribosomal	streptamidine	Streptomyces albidoflavus J1074
BGC0000590.5	0.50	ribosomal	microcin N	Escherichia coli
BGC0002698.2	0.49	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0002144.3	0.47	ribosomal	phazolicin	Rhizobium sp. Pop5
BGC0000483.5	0.47	ribosomal	aeruginosamide B, aeruginosamide C	Microcystis aeruginosa PCC 9432
BGC0002551.2	0.47	other (other), ribosomal, terpene	muscoride A, muscoride B	Nostoc sp. UHCC-0398
BGC0002485.2	0.46	other (other), ribosomal	muscoride A, muscoride B	Desmonostoc sp. PCC 7906
BGC0002306.2	0.45	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0000475.5	0.43	ribosomal	patellamide A, patellamide C	Prochloron didemni
BGC0002699.2	0.43	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 22.05 out of 22.40

Confidence: strong

Additional weak hits (4):

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 19.47 out of 30.97

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.42 out of 33.73

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 21.94 out of 31.73

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.78 out of 30.98

Confidence: weak

(Thi)azol(in)e containing peptide predictions

No core peptides found.

TFBS Finder

Azole RiPPs

TFBS definitions

Regulator	Description
HypR	L-hydroxyproline utilization repressor

Core peptide predictions

No core peptides found.

NZ_LR027842.1 - Region 8 - lanthipeptide-class-iv

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Location: 2,669,166 - 2,691,754 nt. (total: 22,589 nt) Show pHMM detection rules used

lanthipeptide-class-iv: (cds(LANC_like and Pkinase) and not micKC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000563.4	0.53	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0001226.4	0.52	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0001579.4	0.46	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0002700.2	0.45	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0002111.3	0.42	ribosomal	andalusicin A, andalusicin B	Bacillus thuringiensis serovar andalousiensis NRRL B-23139
BGC0001660.3	0.42	ribosomal	mathermycin	Marinactinospora thermotolerans
BGC0001555.4	0.41	ribosomal	colicin V	Escherichia coli chi7122
BGC0000503.5	0.41	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002699.2	0.40	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102
BGC0002005.2	0.40	ribosomal	RaxX	Xanthomonas oryzae pv. oryzae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000563.4	0.53	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0001226.4	0.52	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0001579.4	0.46	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0002700.2	0.45	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0002111.3	0.42	ribosomal	andalusicin A, andalusicin B	Bacillus thuringiensis serovar andalousiensis NRRL B-23139
BGC0001660.3	0.42	ribosomal	mathermycin	Marinactinospora thermotolerans
BGC0001555.4	0.41	ribosomal	colicin V	Escherichia coli chi7122
BGC0000503.5	0.41	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002699.2	0.40	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102
BGC0002005.2	0.40	ribosomal	RaxX	Xanthomonas oryzae pv. oryzae

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.36 out of 23.79

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.96 out of 20.97

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.81 out of 24.78

Confidence: medium

Additional weak hits (4):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.75 out of 32.74

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.81 out of 27.55

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.08 out of 32.74

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 22.46 out of 30.12

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

No core peptides found.

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
IolR	Regulator of the inositol catabolism
LexA variant 2	Repressor of DNA damage response

NZ_LR027842.1 - Region 9 - NRPS-like,T1PKS,prodigiosin

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Location: 2,744,718 - 2,791,953 nt. (total: 47,236 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
prodigiosin: (PPDK_N and AMP-binding and PP-binding and PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

E0E39_RS12295: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: PPTase methyltransferase

E0E39_RS12310: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

E0E39_RS12340: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Hydrolases: esterase

E0E39_RS12315: thioesterase

smcogs: SMCOG1004: thioesterase

Isomerases: mutase

E0E39_RS12365: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: ligase synthase

E0E39_RS12300: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

E0E39_RS12320: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

E0E39_RS12325: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

E0E39_RS12330: 8-amino-7-oxononanoate synthase

smcogs: SMCOG1109: 8-amino-7-oxononanoate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001164.3	1.08	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	1.07	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001276.3	0.89	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.89	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.89	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.88	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000307.4	0.88	NRPS	AbT1	Aureobasidium pullulans
BGC0001166.5	0.87	NRPS	HC-toxin	Alternaria jesenskae
BGC0001284.4	0.84	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002164.2	0.82	NRPS	peramine	Epichloe festucae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001164.3	0.69	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.69	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001276.3	0.63	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.63	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.63	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000307.4	0.63	NRPS	AbT1	Aureobasidium pullulans
BGC0001166.5	0.63	NRPS	HC-toxin	Alternaria jesenskae
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002164.2	0.61	NRPS	peramine	Epichloe festucae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.23 out of 19.52

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.38 out of 25.88

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.77 out of 29.72

Confidence: medium

Additional weak hits (8):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.44 out of 32.74

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 19.62 out of 27.78

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.42 out of 33.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.37 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.72 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.31 out of 26.06

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.29 out of 25.75

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 23.31 out of 33.14

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 13, location 2744717 - 2791953:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (NH2)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

E0E39_RS12320: NH2 - mal

CAL_domain (52..490): NH2

minowa: NH2

Prediction, score:

NH2: 96.1
AHBA: 24.9
fatty_acid: 16.8
shikimic_acid: 14.3
Acetyl-CoA: -0.1

PKS_AT (1244..1524): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 66.7%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 56.2
Methylmalonyl-CoA: 44.5
Methoxymalonyl-CoA: 39.8
inactive: 24.5
Propionyl-CoA: 22.6
CHC-CoA: 17.8
2-Methylbutyryl-CoA: 10.7
trans-1,2-CPDA: 10.5
fatty_acid: 10.0
Ethylmalonyl-CoA: 7.1
Isobutyryl-CoA: 2.6
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

E0E39_RS12325: Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (31..408): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FWCAFDLSTHDVYSLFAGEGNLYGPTETNVCLAS
Nearest Stachelhaus code(s):: Pro DLFYLALVCK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
NtrC	Nitrogen regulatory protein C
PsrA	Oleic acid responsive regulator

NZ_LR027842.1 - Region 10 - lanthipeptide-class-ii

Download region SVG

Location: 2,851,585 - 2,874,506 nt. (total: 22,922 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: deacetylase

E0E39_RS12760: acetylornithine deacetylase

smcogs: SMCOG1247: acetylornithine deacetylase

Lyases: cyclase

E0E39_RS12740: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Ligases: synthase

E0E39_RS12700: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

Translocases: transporter

E0E39_RS12775: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001535.3	0.57	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0001555.4	0.51	ribosomal	colicin V	Escherichia coli chi7122
BGC0001579.4	0.50	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0002698.2	0.50	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001210.5	0.50	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000626.3	0.49	ribosomal	thuricin	Bacillus thuringiensis
BGC0000588.5	0.48	ribosomal	microcin L	Escherichia coli
BGC0002699.2	0.48	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102
BGC0000539.5	0.47	ribosomal	nukacin ISK-1	Staphylococcus warneri
BGC0000521.3	0.46	ribosomal	lacticin 481	Lactococcus lactis subsp. lactis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001535.3	0.57	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0001555.4	0.51	ribosomal	colicin V	Escherichia coli chi7122
BGC0001579.4	0.50	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0002698.2	0.50	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001210.5	0.50	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000626.3	0.49	ribosomal	thuricin	Bacillus thuringiensis
BGC0000588.5	0.48	ribosomal	microcin L	Escherichia coli
BGC0002699.2	0.48	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102
BGC0000539.5	0.47	ribosomal	nukacin ISK-1	Staphylococcus warneri
BGC0000521.3	0.46	ribosomal	lacticin 481	Lactococcus lactis subsp. lactis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for E0E39_RS12740

E0E39_RS15855

Leader:

MSDMNFLKEVADHELSQSEKTQAGG

Core:

LWTVPGTRTIGAVCTVSWECHGFICGLFK

Core with dehydrations:

LWDhbVPGDhbRDhbIGAVCysDhbVDhaWECysHGFICysGLFK

antiSMASH-DB 4.0 matches for E0E39_RS15855

100.0%

BGC0000517.4 (BAB04173.1):

Lanthipeptides: E0E39_RS15855_lanthipeptide

1

LWTVPGTRTIGAVCTVSWECHGFICGLFK

29

LWTVPGTRTIGAVCTVSWECHGFICGLFK

1

LWTVPGTRTIGAVCTVSWECHGFICGLFK

29

62.1%

NZ_CP035495.1and 1 other:

NZ_CP035495.1 Lanthipeptides: ET495_RS17535_lanthipeptide

Lanthipeptides: ET495_RS17535_lanthipeptide

1

LWTVPGTRTIGAVCTVSWECHGFICG

26

LWT+PGT TIG CT+SWEC G CG

1

LWTIPGTSTIGRFCTLSWECQGIACG

26

55.9%

NC_014643.1and 2 others:

NZ_KV795763.1 Lanthipeptides: HMPREF2554_RS11600_lanthipeptideNZ_KV804818.1 Lanthipeptides: HMPREF2804_RS11135_lanthipeptide

Lanthipeptides: HMPREF0733_RS11155_lanthipeptide

1

LWTVPGTRTIGAVCTVSWECHGFICG

26

L+TVPGT T+G CT+SWEC GFICG

8

LFTVPGTTTVGHFCTISWECSGFICG

33

with 52 more sequence matches

Only the first 10 are displayed here.

55.2%

NZ_CP025227.1:

Lanthipeptides: allorf_0999194_0999376_lanthipeptide

1

LWTVPGTRTIGAVCTVSWEC

20

LWTVP T TIG VCT+SWEC

1

LWTVPTTSTIGLVCTLSWEC

20

53.1%

NZ_CP051884.1and 2 others:

NZ_CP051884.1 Lanthipeptides: HGK68_RS15160_lanthipeptideNZ_CP051884.1 Lanthipeptides: HGK68_RS15160_lanthipeptide

Lanthipeptides: HGK68_RS15160_lanthipeptide

1

LWTVPGTRTIGAVCTVSWECHGFIC

25

+WT+PGT+TIGA CT+S+EC GF+C

5

IWTIPGTKTIGAFCTISYECWGFVC

29

51.6%

NZ_QGGR01000002.1:

Lanthipeptides: BC793_RS05490_lanthipeptide

1

LWTVPGTRTIGAVCTVSWECH

21

L+TVPGT TIG +CTVSWEC

1

LYTVPGTSTIGWICTVSWECS

21

50.0%

NZ_JAPVBQ010000001.1:

Lanthipeptides: O2V63_RS05740_lanthipeptide

1

LWTVPGTRTIGAVCTVSWECHGFICGLF

28

L+T T+T+GAVCT+SWEC G ICG+F

8

LFTFKTTKTVGAVCTISWECSGRICGIF

35

48.4%

NZ_PVMZ01000043.1and 1 other:

NZ_PVMZ01000043.1 Lanthipeptides: CLV67_RS49720_lanthipeptide

Lanthipeptides: CLV67_RS49720_lanthipeptide

1

LWTVPGTRTIGAVCTVSWEC

20

L+TVPGT TIG VCT S+EC

1

LFTVPGTSTIGYVCTYSYEC

20

37.9%

NZ_KZ454939.1:

Lanthipeptides: CEF20_RS01155_lanthipeptide

2

WTVPGTRTIGAVCTVSWEC

20

WT+PG G VCTV+ EC

9

WTLPGG---GGVCTVTSEC

24

34.5%

NZ_BORO01000007.1:

Lanthipeptides: KI343_RS07745_lanthipeptide

2

WTVPGTRTIGAVCTVSWEC

20

WT+PG G VCT++ EC

9

WTLPGG---GGVCTLTSEC

24

34.5%

NZ_CP022163.1:

Lanthipeptides: MEBOL_RS24465_lanthipeptide

4

VPGTRTIGAVCTVSWECHGF

23

VPG + G +CT S EC F

7

VPG-NSGGWICTYSTECKPF

25

34.5%

NZ_JALASB010000016.1and 3 others:

NZ_JALASR010000012.1 Lanthipeptides: MOD87_RS16305_lanthipeptideNZ_JALAUW010000005.1 Lanthipeptides: MOE83_RS03160_lanthipeptideNZ_JALAXQ010000010.1 Lanthipeptides: MOF24_RS12150_lanthipeptide

Lanthipeptides: MOD42_RS18070_lanthipeptide

2

WTVPGTRTIGAVCTVSWEC

20

WT+PG G VCT++ EC

9

WTLPGG---GGVCTITSEC

24

31.0%

NC_017912.1and 14 others:

NZ_CP007242.1 Lanthipeptides: KHU1_RS17410_lanthipeptideNZ_CP031424.1 Lanthipeptides: DXX91_RS18935_lanthipeptideNZ_CP032855.1 Lanthipeptides: D9C11_RS10440_lanthipeptideNZ_CP043809.1 Lanthipeptides: ETZ92_RS14260_lanthipeptideNZ_CP049904.1 Lanthipeptides: V753_RS18470_lanthipeptideNZ_CP053764.1 Lanthipeptides: HPQ66_RS18325_lanthipeptideNZ_CP061938.1 Lanthipeptides: IEN91_RS19800_lanthipeptideNZ_CP063768.1 Lanthipeptides: IM712_RS05205_lanthipeptideNZ_CP087137.1 Lanthipeptides: LOK79_RS18580_lanthipeptideNZ_CP092383.1 Lanthipeptides: MID02_RS19390_lanthipeptideNZ_JAEACM010000001.1 Lanthipeptides: I5F15_RS04355_lanthipeptideNZ_JAJNPN010000015.1 Lanthipeptides: LQ568_RS13285_lanthipeptideNZ_SDKF01000002.1 Lanthipeptides: EUA41_RS09950_lanthipeptideNZ_VDUT01000007.1 Lanthipeptides: FS412_RS14545_lanthipeptide

Lanthipeptides: MUS_RS20065_lanthipeptide

2

WTVPGTRTIGAVCTVSWEC

20

+T+PG G VCT++ EC

9

FTLPGG---GGVCTLTSEC

24

MIBiG 4.0 matches for E0E39_RS15855

20.7%

Lanthipeptide: haloduracin β or haloduracin α

11

GAVCTVSWEC

20

GA CT++ EC

14

GAYCTLTVEC

23

16.7%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

11

GAVCTVSWECH

21

G CT++ EC

16

GGWCTLTKECQ

26

12.8%

BGC0001229.4 (SBI_06990):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

14

CTVSWECH

21

CT+S EC

28

CTLSKECQ

35

allorf_2854625_2854753

Leader:

MRGRGTASGGGWVEPTNLVGG

Core:

IVGSRPSEETYAPVGYGCVGF

Core with dehydrations:

IVGDhaRPDhaEEDhbYAPVGYGCysVGF

antiSMASH-DB 4.0 matches for allorf_2854625_2854753

100.0%

Lanthipeptides: allorf_2854625_2854753_lanthipeptide

1

IVGSRPSEETYAPVGYGCVGF

21

IVGSRPSEETYAPVGYGCVGF

1

IVGSRPSEETYAPVGYGCVGF

21

19.0%

NZ_CP041611.1:

Lanthipeptides: allorf_00810301_00810489_lanthipeptide

2

VGSRPSEETYAPVGYG

17

VG RP + PVG G

13

VGGRPCDRPTRPVGGG

28

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 25.59 out of 27.78

Confidence: strong

Additional weak hits (6):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.38 out of 21.84

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.67 out of 27.78

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.78 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 18.09 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 19.06 out of 26.06

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

E0E39_RS15855 - Class II

Cleavage pHMM score: -6.30

RODEO score: 0

Monoisotopic mass: 3090.6 Da

Molecular weight: 3092.6 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3110.7 Da N = 1; 3128.7 Da N = 2

allorf_2854625_2854753 - Class II

Cleavage pHMM score: -3.40

RODEO score: 0

Monoisotopic mass: 2133.0 Da

Molecular weight: 2134.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2152.4 Da N = 1; 2170.4 Da N = 2

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria

NZ_LR027842.1 - Region 11 - lanthipeptide-class-iii

Download region SVG

Location: 3,105,770 - 3,128,559 nt. (total: 22,790 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

E0E39_RS13950: nucleotide sugar dehydrogenase

smcogs: SMCOG1152: nucleotide sugar dehydrogenase

Transferases: glycosyltransferase

E0E39_RS13970: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000513.3	0.54	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000563.4	0.52	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0000551.5	0.52	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000501.3	0.51	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000519.4	0.51	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000496.5	0.51	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002698.2	0.45	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0002701.2	0.44	ribosomal	paenithipeptin	Paenibacillus thiaminolyticus
BGC0000504.5	0.44	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000500.5	0.43	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000513.3	0.54	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000563.4	0.52	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0000551.5	0.52	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000501.3	0.51	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000519.4	0.51	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000496.5	0.51	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002698.2	0.45	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0002701.2	0.44	ribosomal	paenithipeptin	Paenibacillus thiaminolyticus
BGC0000504.5	0.44	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000500.5	0.43	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.65 out of 18.46

Confidence: strong

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 25.32 out of 29.65

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.33 out of 24.78

Confidence: medium

Additional weak hits (8):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.15 out of 21.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.54 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.83 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.16 out of 27.78

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.32 out of 24.78

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.33 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.42 out of 24.78

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.86 out of 29.65

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

No core peptides found.

TFBS definitions

Regulator	Description
LexA variant 1	Repressor of DNA damage response
LexA variant 2	Repressor of DNA damage response
RpoN	Regulator of nitrogen assimilation and virulence

NZ_LR027842.1 - Region 12 - azole-containing-RiPP