LOCUS       NZ_LS992241            11956 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Paenibacillus alvei isolate Paenibacillus B-LR1 chromosome PBLR.
ACCESSION   NZ_LS992241
VERSION     NZ_LS992241.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus alvei
  ORGANISM  Paenibacillus alvei
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1
  AUTHORS   Chevrot,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2018) UMR CNRS 7266, LITTORAL ENVIRONNEMENT ET
            SOCIETES, Batiment Marie Curie, Avenue Michel Crepeau, La Rochelle,
            France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:34:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,912
            CDSs (total)                      :: 5,797
            Genes (coding)                    :: 5,720
            CDSs (with protein)               :: 5,720
            Genes (RNA)                       :: 115
            rRNAs                             :: 10, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 39 of 77
            Pseudo Genes (incomplete)         :: 49 of 77
            Pseudo Genes (internal stop)      :: 4 of 77
            Pseudo Genes (multiple problems)  :: 14 of 77
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:26:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1841539
            Orig. end                         :: 1853495
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LS992241.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11956
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11956
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11956
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6956](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6956
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            207..641
                     /locus_tag="PBLR_RS08240"
                     /old_locus_tag="PBLR_11707"
     CDS             207..641
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005549772.1"
                     /locus_tag="PBLR_RS08240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PBLR_11707"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_232055529.1"
                     /transl_table=11
                     /translation="MNNMNTPVNLKGDSDWEQVRELLASCMYPNEEKINRQVVLYRSDE
                     SLYLYGVVIQSELVGLIGMGVGENEEGVLRHIVVKPHLRGQGIGHTMIKHMMQILNLLR
                     LEAETDRDAVGFYQKAGFAVTSLGEKYPGVERFLCVLQSY"
     gene            763..1635
                     /locus_tag="PBLR_RS08245"
                     /old_locus_tag="PBLR_11708"
     CDS             763..1635
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /locus_tag="PBLR_RS08245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PBLR_11708"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_138185407.1"
                     /transl_table=11
                     /translation="MPNQTLAVEQLRITDTQGLIDLSASIGWDYDEQDIQTIMSSGTVW
                     GHRNEQGDIISSAAILTYKPGFSTIGMVIVDQRYRGMGLGKAVVQACMDAVDNSDTIML
                     IATEEGRPLYEGMGYHTVTYVNKLVCSSYDSTLSNWSGSVSEMRTMPLGLEHFPEIREL
                     DQLAIGADRETFLSARWKQAKQGVVVLDNEGTLIGFGFAVQGPKYLVLGPVVAVQQEAA
                     SAMIHYLAGDNRGKLLRIDVPEEQPEFIELLIQAGFERETHPPVMIKNAQQLPTRNHTL
                     YGIAAQIFG"
     gene            1650..1799
                     /locus_tag="PBLR_RS29375"
                     /old_locus_tag="PBLR_11709"
     CDS             1650..1799
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PBLR_RS29375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PBLR_11709"
                     /product="hypothetical protein"
                     /protein_id="WP_172619445.1"
                     /transl_table=11
                     /translation="MEWSALIISFFFLATLVRIEINTKKKLDNDKKIMKQLQDILDELK
                     KMKN"
     gene            1813..2109
                     /locus_tag="PBLR_RS30570"
                     /old_locus_tag="PBLR_11710"
     CDS             1813..2109
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /locus_tag="PBLR_RS30570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PBLR_11710"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_332018246.1"
                     /transl_table=11
                     /translation="MISSFPRLETDRMILRQLRSEDAANLFENFSTDEVTQYYNLDNFV
                     AIEQAEQLIERWNQRFEEGQSIRWGIALQSEDRIIGTCGFHNWRRADCKAEIG"
     gene            2142..2378
                     /locus_tag="PBLR_RS30575"
                     /old_locus_tag="PBLR_11711"
     CDS             2142..2378
                     /EC_number="2.-.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /locus_tag="PBLR_RS30575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PBLR_11711"
                     /product="GNAT family protein"
                     /protein_id="WP_332018247.1"
                     /transl_table=11
                     /translation="MTEALNRIVAFGFEEMQLNRIEAYVFGTNTASQKLLEKSGFNQEG
                     LLQECYRKNDSFIDAILFAKLQKDYAENVNNIR"
     gene            2485..3219
                     /locus_tag="PBLR_RS08255"
                     /old_locus_tag="PBLR_11712"
     CDS             2485..3219
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PBLR_RS08255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PBLR_11712"
                     /product="hypothetical protein"
                     /protein_id="WP_138185409.1"
                     /transl_table=11
                     /translation="MKKIISIVVVVLLLSFVFFVYTEVEGLPWKHAEVKRKAIDYMKQK
                     YNMDVAAAGSIYNFKFKIYTAKVYNVKDSNKAIIRVDDESDFDDEDNYVGKRLQDDYTL
                     VYWSESIRDGLQAKYPDLFKLQDIESIQIEYAYYTLSLTEGLSGDQDQNGVHIPVKPKD
                     VPELYIRLKSVDIGDHVLEQLREVINDMVGTSQPMGVFVRAAEDKRTQKNEMSKTEMLH
                     LEHDQLKPIQSIEDLKKNIKLL"
     misc_feature    complement(3312..3327)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.03"
                     /tool="antismash"
     gene            3424..5004
                     /locus_tag="PBLR_RS08260"
                     /old_locus_tag="PBLR_11713"
     CDS             3424..5004
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PBLR_RS08260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PBLR_11713"
                     /product="hypothetical protein"
                     /protein_id="WP_138185411.1"
                     /transl_table=11
                     /translation="MSKQGQGVLAIGSGSMLDSLVKTWHEAGDFTLTVCTTDTETPDHV
                     AFTVHQEPDEQGRLTSNVTMNTERRDLKQDWCRIIRPYRFILYAAQLENLAEWRDLQDA
                     CELEGKPLLPAITLRGVGLVGPLLDVDGSARPEAVWRNLHPTVFRSENEQLCFSLTALH
                     ILTNLIVHEYHRELSAGREGNCRDQCYILDPNSLTGSWHSIRSHLVDVERGSKAACRIS
                     AVEEVITASNVRMNESDWYTSFADMTSSSIGIFHKWEEAGIVQLPLAQCMVQPIDPASE
                     SFVRLLPAIMCSGLTHEEARRESGLAGLEAYAARLLPFLFPVSGNDHVADISVGAGCEA
                     AEAVGRGIRACLTKRLQDRIDRSRHGTSQMIVSRLRYEHVADSRCEYYLQSLRIMGNES
                     IIAVGEPLLGYPVVWVYSRDNWYGSVDVDFTIALRHSLQKALNQVEGHASDLFLVQCER
                     DNSMNTLAIPHIAYPFTSAYWIATVQRVLEKNKKDDSCFEIYDLHSSSFLGTEAFFVCG
                     VRLREEGST"
     gene            5001..6956
                     /locus_tag="PBLR_RS08265"
                     /old_locus_tag="PBLR_11714"
     CDS             5001..6956
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005549760.1"
                     /locus_tag="PBLR_RS08265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PBLR_11714"
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="WP_172619446.1"
                     /sec_met_domain="YcaO (E-value: 1.5e-76, bitscore: 256.6,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 4.9e-118, bitscore:
                     392.6, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTVLVIGEGLLADVVYAQLMDQCNYNVARLTNHNEVIPSTSISI
                     ALVLQDQENLGLLLDVNEKLQQGGIPWISAYLSEEEGFVGPMNWPGASGCFQCADIRMS
                     IAGSNTNATDDMLMMLVNPDYVPRVEPEIEVTGYRYMAQLLVEETSKFVNGHSLCTDSH
                     IYMINLHSLNCTRHYILPHGACPVCGQLEEDSPEAASIVLKPSPKLDNSYRCRSMNELQ
                     GVLFNDYWDHRSGLFNDIELHLSSVFADAVVNLPLGYYDEVTAGRSHSFMNSQLAAILE
                     GLERYCGITPRGKRPVVFDSYNQLMEVAMDPASIGLHAQEQYEQEGFPFASFDPDTPIE
                     WVWGYSFLQERPILVPHLVAYYSLGHEGSFVYETSNGCAIGGSLEEAVLYGIFEVVERD
                     AFLMTWYAKLQRPRLDPYSSGDEELLLMIQRIKAVLGYEVLLYNVTTENGIPSIWAIAK
                     GAAEHEVSVVCSAGAHLDPIRAAKSAVHELAGTISMVEKRWKERGSEALSMFMDSYEVQ
                     LMEDHTLLYGLPEAAERLQFLLEEQRPVRSFAEEFPTPVVPVSTDVTEELKEVLEVFRR
                     LQLDVIVVDQSSSETLRNGLHCVKVLIPGMLPMTFGHHLRRLEGIDRVLEVPMKLGYVD
                     RKLIREELNPYPHPFP"
     gene            7173..7796
                     /gene="yqeK"
                     /locus_tag="PBLR_RS08270"
                     /old_locus_tag="PBLR_11715"
     CDS             7173..7796
                     /EC_number="3.6.1.41"
                     /codon_start=1
                     /gene="yqeK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424199.1"
                     /locus_tag="PBLR_RS08270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PBLR_11715"
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="WP_138185414.1"
                     /transl_table=11
                     /translation="MHLLLDKLVQQVEFTGNIEQNIQRFLTANHCSHTADHSRDVAAEA
                     GRVARIYGADPERAELAGWLHDISAVFPNEERIEAARQLGLDILPEEEQFPMIIHQKLS
                     KVMARDIFGVQDAEVLDAVGCHTTLRADASQLDLVLFVADKIAWDQQGTPPYIEELHHN
                     LAISLEHAAFAYIQYLWERKDTLKVVHPWLADAYEQLHVQLNAE"
     gene            8063..9379
                     /locus_tag="PBLR_RS08275"
                     /old_locus_tag="PBLR_11716"
     CDS             8063..9379
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PBLR_RS08275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PBLR_11716"
                     /product="J domain-containing protein"
                     /protein_id="WP_138185416.1"
                     /transl_table=11
                     /translation="MNVWDVLGIEPTEDDSVISSAYEKKLTLEHPENDPQWKEMLHEAY
                     EVALKLARKRSAATEASSMRLNDHGERLENTYENMENAREEIAVSTAYPYDANPIQIFL
                     GQVSGLYSNFANRIQPSSWQALFQENAEWDIENPQLQDEMVMFLEEKHYFPMAVWAVLE
                     KTFRFQEHEEELRGRHAEEFIDYIVEQVNGTVELTYDCFMDVKIDFDIDRYLYLREMAQ
                     YMLMAGELEEVGAYLSEAHELFQDDPDLELMRGKYFEAIGEMEQAMDCFKQVIAMKPDE
                     FEGRWHRAQLLYKLEQYEAALADCNSLLEIEGNQQDALMLSIHCYTQLGQLKEARMKLQ
                     QSNDIDNVHDILRYVHGIKLFNKYRYTQEVEEKGQRQARKQISSFLLYFYLRLTWPYIL
                     VYVGLQFFISVPIAVTVLWLAVMVWSGWKTFKTYRMMTT"
     gene            9395..10057
                     /locus_tag="PBLR_RS08280"
                     /old_locus_tag="PBLR_11717"
     CDS             9395..10057
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF018584.4"
                     /locus_tag="PBLR_RS08280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PBLR_11717"
                     /product="DUF1266 domain-containing protein"
                     /protein_id="WP_138185418.1"
                     /transl_table=11
                     /translation="MPSEYNNQQRKQLELYFRCLSSVCTDSVMGYHYIPFGFPFTDRIL
                     RRGSMKKAFAAWGVTDKESLLSRVQWLLEDGLREEYKKLHTSLAARNEVSRKRYVESSV
                     DHPDHAKVVIVNAFLHQLPSGEIAAFGGAWAIFMCNMGLTYGYLTKDEAWGLKIKAARH
                     LQQYYNSWEDYLTAFVAGNSYSDSDSEMKMQAHITSIASMLLGSSTLAMDKAQWGLA"
     gene            10102..10605
                     /locus_tag="PBLR_RS08285"
                     /old_locus_tag="PBLR_11718"
     CDS             10102..10605
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005549756.1"
                     /locus_tag="PBLR_RS08285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PBLR_11718"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_232055530.1"
                     /transl_table=11
                     /translation="MNIVNITLHLVEYEQKSTLRNLLELYKYDFSEYDPEDVNENGLYE
                     YMYLDHYWTEEGRYPFFIRVDGKLAGVALVRKLGADDDKRTVYSMAEFFVMKKYRRSGA
                     GRYAATALFDRFQGTWKVGQIDENVPSQHFWRKVITAYTNDNYEEIRENGWDGPIQCFS
                     TELR"
     gene            complement(10772..11227)
                     /locus_tag="PBLR_RS08290"
                     /old_locus_tag="PBLR_11719"
     CDS             complement(10772..11227)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1120: AsnC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005549754.1"
                     /locus_tag="PBLR_RS08290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PBLR_11719"
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="WP_138185420.1"
                     /transl_table=11
                     /translation="MQFRNLDEIDTRILQLLSENGRLSNAELGRMVNLTRAAVRERIHQ
                     LMDDGIIDRFTIVVNPLKAGKTLSMYFDIEVDWVKLQHVVEQLRLAEEITNVYQMSGKP
                     HLHVHALLDDPEHAEQCLRMLQAIDGITSVTSEVLIARHKERGGLLI"
ORIGIN
        1 ctaactttca tagtagggat atacggtatg aactttgata acatgcctga gcttcattgg
       61 caatggggtt attttatcgt tttgggagtt ctcgttggtg gcgggattgg aatgtatgcg
      121 tggtttaaaa gaaaaggctg gtttgattga aaactggagt aatgacttca agaaaataag
      181 agtcgctata gggaggccgc aacaacatga acaacatgaa tacaccagtt aatctcaagg
      241 gggattcgga ttgggagcag gtgcgcgaat tgcttgcatc atgtatgtac ccgaacgaag
      301 agaagatcaa tagacaggtt gttctctacc gaagcgatga gtccctgtac ctgtatggag
      361 ttgtcattca atcagagctg gtcggattaa ttggtatggg tgtaggtgag aatgaagaag
      421 gcgtactgcg tcatatcgtg gtaaagccgc accttcgtgg tcagggaatt ggacatacga
      481 tgatcaaaca tatgatgcag atcctcaatc ttcttagact tgaagcagaa acggatcgag
      541 atgcagttgg gttctatcag aaagctggct ttgctgttac aagtcttggt gaaaaatatc
      601 cgggtgtgga gcgattttta tgcgtgttac agagttatta ggtgtataat aatggaataa
      661 ttgaatttcg accttagact cagccctaca gaagcatgat cttgtaaagc tgggttcttt
      721 tatttctatt tctaaagtgt gctcagaaag gtgatagatt tgatgccgaa tcagacatta
      781 gctgtagagc aattgcgtat aacagatacg cagggattga ttgacctatc cgcttcgatt
      841 ggttgggatt atgatgaaca ggatattcag acgattatgt catctggcac ggtatgggga
      901 catcgcaatg agcaaggcga cattatttca agcgctgcta ttcttaccta caagcctgga
      961 ttttctacca ttggtatggt tatcgttgat caacgatata gagggatggg attagggaag
     1021 gcggttgttc aagcttgtat ggacgctgtt gataacagtg acaccattat gctgattgca
     1081 acggaagaag gccgaccttt gtatgaaggc atgggatatc ataccgtaac gtacgtcaat
     1141 aaacttgtat gttctagcta cgattccaca ttatctaatt ggagtggttc tgtctctgaa
     1201 atgcgtacca tgccgctagg gttggaacac tttcccgaga ttcgtgagct ggatcagttg
     1261 gccattggag cagatagaga gacattccta tctgctcgct ggaaacaggc gaagcagggg
     1321 gtagtcgtac tggataacga aggtactctg atcgggtttg gatttgccgt tcaaggcccg
     1381 aagtatctcg ttcttggacc agtggtagct gtacagcagg aagcggcatc agccatgatt
     1441 cattatttgg caggagataa ccggggcaag ctgcttcgta ttgatgttcc agaggaacaa
     1501 cctgaattta tcgagctgtt gatacaggct ggttttgaga gagagaccca tcctccggtg
     1561 atgattaaga atgcacaaca gctgccgaca agaaatcata cgctatatgg aattgcggcg
     1621 caaatatttg gataacgggt ggaatgaaca tggaatggtc tgctcttatt atttcgtttt
     1681 tcttcttagc tacactcgtc agaattgaaa ttaacaccaa gaagaagctt gataatgata
     1741 agaagataat gaaacagctt caggatatct tggatgaatt aaagaaaatg aagaattgaa
     1801 ggaggaagta agatgatatc atcatttcct aggctagaaa cggacagaat gattctgcgg
     1861 cagctcaggt cggaggatgc ggccaatcta ttcgagaact tctcaacgga cgaagtaacg
     1921 cagtattata acttggataa tttcgtggcg attgaacaag ccgaacaatt aatagagaga
     1981 tggaaccaac gattcgagga agggcagagc attcgctggg gaattgcact tcaatcggag
     2041 gatagaatca ttgggacttg cggctttcac aactggcgga gagcagattg caaagctgag
     2101 atcggataga attgaatcca aaccattgga gtaagggcct gatgacggaa gcattgaacc
     2161 gcatagtcgc atttggattt gaggagatgc aattaaaccg aatcgaagcc tatgtgtttg
     2221 ggacaaatac ggcttcacaa aaactgcttg agaagtccgg attcaaccaa gaaggattgc
     2281 tgcaggagtg ctatcgcaaa aatgacagtt ttatagatgc tattctattt gccaagcttc
     2341 aaaaggatta tgcagaaaac gtaaataata taagatgaag tgatactgtg cactccatac
     2401 tcccatgtag tacacgaatc agataagcgg agatgtgaga acaatgaagc tagtgtgtac
     2461 aattcggtca ggtaggtgtt gtgcatgaag aaaataatat cgattgtggt tgttgttttg
     2521 cttttgtcct ttgtgttttt cgtctatacg gaagttgaag gattgccttg gaaacatgct
     2581 gaagtaaagc ggaaggcaat cgattatatg aaacaaaagt acaatatgga tgttgcagcg
     2641 gcaggaagta tttataattt taaatttaaa atatatacag cgaaagtata taacgtaaag
     2701 gattcgaaca aagcgatcat tcgtgtggac gatgaaagtg acttcgacga cgaggacaac
     2761 tatgttggaa agcgattgca agacgattat acccttgtgt attggtctga atctattcgt
     2821 gatggactgc aggcaaaata tcctgatctg ttcaagcttc aagatattga atcgattcag
     2881 attgaatacg catattacac attatcactt accgaagggc tcagcggtga tcaggatcag
     2941 aacggggtgc atattccggt aaagcccaag gatgttcctg aattgtacat tcggttgaag
     3001 agtgtcgata taggcgacca tgtattggag caattgcgtg aggtaatcaa tgatatggtg
     3061 ggaacctctc aaccaatggg tgtcttcgtt agggcagctg aggataaaag gactcaaaaa
     3121 aatgaaatgt caaagaccga aatgcttcat ctagagcacg atcaactcaa gcctatccaa
     3181 tccattgaag atttgaagaa gaacataaag ctcctgtaag ggaacgcagg gctgtctcaa
     3241 ctgtcattac gctaatgaca tgaggcagct ctttttttgt tgcagtagga ctgggccgta
     3301 cgcgagtcat agtgaaacag acgttgacat acgttaacaa tgacatttct aggtcggatt
     3361 cacgataacg aagcgtatcg atttcaatga aatataggtg tttgtgggaa aggaggattg
     3421 tttatgtcta agcaaggaca gggggtactt gctattggct cgggatccat gctggattca
     3481 ttggtaaaaa cctggcatga agcgggagac ttcacactga cggtctgcac aactgacacg
     3541 gaaactcccg atcatgttgc gtttacggta catcaggagc ctgacgaaca gggaagattg
     3601 acctcaaatg tcacgatgaa tactgaacgt agagatctca aacaagattg gtgccgtatt
     3661 attcgaccat accgttttat cctatacgct gctcagcttg agaatttggc tgaatggaga
     3721 gaccttcagg atgcatgtga attggagggg aaacctctac tgcctgcgat aaccttacgt
     3781 ggggttggat tagttggacc attgttagat gtggacggat cagcacgacc agaagcggtg
     3841 tggcggaacc tgcatccgac cgttttccga agtgagaatg agcagctgtg cttctcgtta
     3901 acggcacttc atattcttac aaatctgatt gtgcatgaat atcatcggga attgtcagca
     3961 ggaagagaag ggaattgtag agatcaatgc tatattcttg atccgaacag cttaacaggg
     4021 agctggcatt ccattcgttc acatctcgtc gatgttgaac gagggagtaa ggcggcttgc
     4081 cgtatatctg cagtggaaga agtcatcaca gcaagtaatg tgcgaatgaa tgaatcagat
     4141 tggtatacca gcttcgccga tatgacttcc tcttcaattg gaatttttca caaatgggaa
     4201 gaggcgggaa ttgtgcagct tcctctcgca caatgcatgg tgcagccgat tgatccggca
     4261 tctgaatcat ttgtccgact actgccagca atcatgtgca gtggattgac acatgaagag
     4321 gctcggcgcg agtcgggact tgctggattg gaagcgtatg cggcacgttt attaccgttt
     4381 ctgttccctg tatcggggaa tgaccatgtg gctgatatca gtgtaggtgc aggttgtgag
     4441 gcagctgaag ctgttggacg gggaattagg gcatgcttga cgaaacgatt gcaggatcgt
     4501 attgaccgca gccgacacgg aacgagtcaa atgatcgtat cgcgactgcg atatgaacat
     4561 gtagcagaca gccgttgtga gtattatttg caatcgctac gtatcatggg aaatgagtct
     4621 attattgcgg tgggggagcc gttgctaggt tatccggtag tatgggtcta ttcgagggat
     4681 aattggtatg gaagtgtgga tgttgatttc acaattgcgc ttcgccattc ccttcagaag
     4741 gcgctaaatc aggttgaggg gcatgcgtcc gatctatttc tagttcaatg cgaaagagat
     4801 aattcaatga acacgctcgc gatccctcat attgcctatc cgttcacatc tgcttattgg
     4861 atagcgacag tgcaaagagt attggagaag aacaagaagg acgattcatg cttcgaaata
     4921 tatgatttgc acagcagttc tttcctgggt acagaggcat tcttcgtatg tggagtgcgg
     4981 ctcagagagg agggctctac gtgaacactg tcctggtgat tggagaggga ttacttgcgg
     5041 atgtcgtata tgcacagtta atggatcagt gtaattacaa cgtagcaaga ttgacaaatc
     5101 acaatgaagt aattccaagt acaagtatta gcatcgcatt ggtgcttcag gatcaagaaa
     5161 atcttggatt gctgttggat gtgaatgaga agcttcagca aggtggaatt ccgtggatat
     5221 cggcttattt gagtgaggaa gaagggttcg tcggtccgat gaattggcca ggtgcatccg
     5281 gatgcttcca atgtgcagac attagaatgt cgatagcggg cagcaacacc aatgcaactg
     5341 atgacatgct tatgatgctg gttaaccctg actatgtccc acgagttgaa ccggagattg
     5401 aagtgacagg gtatcgatat atggctcagt tacttgttga ggagacatcg aaattcgtaa
     5461 acggacacag tctgtgtacc gacagtcaca tttatatgat caacttgcac agcttgaact
     5521 gtacgcggca ttatattctt cctcatggtg cttgtccagt atgcggccag ctagaagaag
     5581 attcaccgga agcagcgagt attgtgctta aaccatctcc caagctggat aatagctatc
     5641 ggtgtcgttc catgaatgaa ttacaggggg ttctgttcaa cgactattgg gatcatcgat
     5701 ccggcttatt taacgacata gagctgcatc tttcttctgt atttgcagat gcagtcgtca
     5761 atctcccact cggctattac gatgaggtga cagcgggacg ttcccattcg ttcatgaaca
     5821 gtcagcttgc tgccatatta gagggattgg aacgttattg cggcatcaca ccccgcggta
     5881 aacgcccagt tgtgttcgat agttacaacc aactgatgga agttgctatg gatcccgctt
     5941 caatagggct tcatgcacaa gaacaatacg aacaggaagg ttttccgttt gcctcctttg
     6001 atccagatac ccctattgaa tgggtgtggg gttattcatt tttacaggag cgcccgattc
     6061 tagttcctca tctagtagcc tattacagct tagggcacga aggaagtttc gtatatgaaa
     6121 cctcaaacgg ctgcgcaata ggaggcagtc tggaagaagc ggtgttgtat ggcatatttg
     6181 aagtagttga acgtgatgcc ttcttaatga cttggtatgc gaagctccaa cgcccaaggc
     6241 ttgatccata ttcctcaggg gatgaagagt tgctcctcat gattcaacgg atcaaggctg
     6301 tcttaggata tgaggtgttg ttgtacaacg taacaacgga gaatgggatt ccaagcatct
     6361 gggcgattgc taagggggca gcagaacatg aagtgagtgt agtctgttct gcaggagctc
     6421 atcttgaccc gatacgagca gctaagagtg ctgtccatga acttgcgggg acgatctcca
     6481 tggttgaaaa acgttggaag gaacgtggtt cggaagcgct gtctatgttt atggattcgt
     6541 atgaggtaca gctgatggaa gaccatactc tgctgtacgg tttgccagaa gcagcggaaa
     6601 ggcttcaatt tctattagag gagcagcgtc ctgtccgttc ctttgccgaa gaattcccaa
     6661 ccccagtagt tccggtaagt acagatgtaa cagaagagtt gaaggaagtt cttgaagtgt
     6721 tccgtcgatt gcaattagac gtgattgttg ttgatcaatc gtccagcgag acgctacgca
     6781 atggcctaca ttgcgtgaaa gtgctgattc caggcatgct tccaatgact tttggccacc
     6841 atctacgtcg attagagggg atcgacagag tgttggaagt accgatgaaa cttggatatg
     6901 tggatcgcaa gcttatacgt gaggaactga acccttatcc acacccattt ccgtaaggga
     6961 tgttgaatga tagaaagcag ttgaaatcaa tgtctgtccg tacatgcaga cggaagaagt
     7021 taaccttcat tcatgtggag gggctgctgg gctggtatct cattgaactt actagtaaaa
     7081 taagtaaatg ataagaagat tatgagaagc agcgattacg gttatgtcga gttcaagata
     7141 tcccatcccc atagtgaaag aggtattacg acatgcatct attattagat aagctcgttc
     7201 aacaagttga gttcacggga aatatcgaac agaatattca acggtttcta accgccaatc
     7261 attgctcaca cacggcagac catagtaggg atgtagctgc ggaagctgga agagttgcac
     7321 gtatatatgg cgctgatcca gagagagcgg aattggcggg atggctgcat gatataagcg
     7381 ccgtatttcc taacgaggaa cgaattgagg cagctaggca gctagggctt gatatactac
     7441 ccgaagaaga gcaattcccg atgatcatcc atcagaagct atccaaggtg atggcccggg
     7501 acatatttgg cgttcaggat gccgaagtgc tggatgcggt gggctgccat actacgctgc
     7561 gagccgatgc ctcacagctt gatcttgtcc tatttgttgc cgacaagatt gcatgggatc
     7621 agcaaggcac tcctccttat atcgaggaat tacatcataa tctcgcaata tcgctggaac
     7681 atgcggcgtt tgcttacatt caatatttat gggagcgcaa ggacacactg aaggtcgtcc
     7741 acccttggct tgcagatgct tatgaacaat tgcatgtaca attgaatgcg gaatgaagtt
     7801 gtaaggtgga gtggtctgac gcagtaggcg tcagaccact ccatttttcg ttgactgcaa
     7861 ccggaaattt tgagagggaa atggggctcc catttcgttc aaatctagta tgaaatggat
     7921 tatctcatgc ggcactggta gaataaagaa tggcagtagg aaatttttca ctgctgctac
     7981 tgatgctgat taaggaaata ttgaaatagc caccgaagca tattgggacg gctggatttg
     8041 agggaaaggt ggatggagta acttgaatgt atgggatgta ttagggattg agccaacgga
     8101 agatgattct gtcatctcaa gcgcttatga gaagaagctg acgctagagc atccagaaaa
     8161 tgacccacag tggaaagaaa tgctgcatga agcgtatgaa gtagcgttga agctggcgcg
     8221 caaaaggtca gcagcaacag aagcttctag catgaggctg aacgatcatg gtgaacggtt
     8281 ggaaaataca tatgaaaata tggagaatgc aagagaagag attgctgtca gcactgctta
     8341 tccgtatgat gcaaatccga ttcagatatt tctaggccaa gtatctgggc tgtatagcaa
     8401 cttcgcaaat cgcattcagc cttcatcttg gcaagcctta ttccaagaga atgcggaatg
     8461 ggatattgag aatccgcagt tacaagatga aatggtgatg tttcttgagg agaagcatta
     8521 ttttccgatg gcggtgtggg ccgtgctgga gaagactttc cgttttcaag agcatgagga
     8581 agaattaagg ggacgccatg ctgaggaatt cattgattat atagtcgaac aggtcaacgg
     8641 tacagtagag ctgacctatg attgctttat ggatgtcaag atcgattttg atattgaccg
     8701 ttatctctac ttacgagaga tggctcagta tatgcttatg gcaggtgaat tggaagaagt
     8761 aggtgcatat ttgagcgaag ctcatgaatt attccaagac gatcctgacc tagagctcat
     8821 gcgcggcaaa tacttcgaag caatcggcga gatggagcaa gcaatggatt gcttcaaaca
     8881 agtgattgcg atgaaacccg atgagtttga gggacgatgg caccgtgcac agctcctgta
     8941 caaacttgaa caatatgaag ctgccttggc ggactgtaat agcttgctgg agattgaggg
     9001 gaatcagcaa gatgcattga tgctgtccat tcattgttat acgcaattag ggcagctgaa
     9061 ggaagcgcgt atgaagctcc aacaatccaa tgacattgac aatgttcatg acattttacg
     9121 gtatgttcac ggtattaagt tatttaacaa atatagatat acccaagaag tggaagaaaa
     9181 gggacagaga caagcacgca aacaaatttc atcatttctc ctctacttct atctccgctt
     9241 gacttggcca tacatactcg tctatgttgg acttcaattt ttcataagcg tacctatcgc
     9301 cgttacagtc ctgtggcttg cagtaatggt gtggagcggt tggaagacgt tcaagactta
     9361 ccgaatgatg actacctaga aggagggctc caatttgcca agcgaataca ataaccaaca
     9421 aagaaagcag ctggagctgt acttccgttg cctttcttct gtatgtacgg acagtgtgat
     9481 ggggtatcat tatattccgt ttggatttcc atttacggat cgaattctgc gcagaggatc
     9541 gatgaaaaag gcgttcgccg cttggggagt tacggacaag gaatcattgc ttagtcgagt
     9601 tcagtggctg ctcgaagatg gactgcgcga ggagtataag aagctgcata cgagtctagc
     9661 tgcacgcaac gaagtctctc gcaagcggta tgtggaatcc tctgtggatc atcctgacca
     9721 tgccaaggtc gtaatcgtca atgcattttt gcaccagctt ccttccggag aaattgcggc
     9781 atttggtggg gcttgggcaa tctttatgtg caatatggga cttacctacg gctacttaac
     9841 caaggatgaa gcttggggct tgaagatcaa ggcggctcgc catttgcagc aatactataa
     9901 cagctgggaa gactatctga ctgcttttgt tgcaggaaat agctactctg actcggatag
     9961 tgaaatgaag atgcaggctc atattacgag tatagcctcg atgctgcttg gatcaagcac
    10021 attggccatg gataaggcac aatgggggtt agcataattc gtctgtaaag ttcattgaaa
    10081 tgaaaacaat gggagagtcg ggtgaatatt gtgaacatca cgcttcatct cgtagagtac
    10141 gagcagaaat ccacgctgcg caatttgcta gagctgtaca aatatgattt cagtgaatac
    10201 gatccagagg atgtcaacga gaacggtcta tatgaatata tgtatctgga tcattactgg
    10261 acagaggaag gaaggtatcc tttcttcatt cgagtggatg ggaagcttgc gggagtagcg
    10321 ctcgtaagga agttaggagc ggatgatgac aagcggacgg tttattcgat ggcggaattt
    10381 ttcgtaatga aaaaatatcg gcggtccggt gcaggaaggt atgcggcaac agctttgttc
    10441 gatcggtttc agggaacttg gaaggtaggt cagatagacg aaaacgtacc ttcgcagcat
    10501 ttttggagaa aagtaattac agcgtataca aacgataact atgaagagat tcgggaaaac
    10561 ggttgggacg gcccgatcca atgcttctcc actgagcttc ggtagaagag agtttccata
    10621 atcgttgtcc aaaaaaatat tttaaaataa catcatcaat tttacgcccc gcttgtggct
    10681 tctggatcgc tgcatacacc agtagcaagc ggggcgttct atatcgattc atgtgaaatt
    10741 gatttcggtg tattatttat agcagatttg tttagatcaa cagtccgcct ctttctttat
    10801 gccgcgcaat gagtacctca ctagtgacgg aagtgatacc gtctatcgct tgaagcatcc
    10861 tcaagcactg ctcagcatgc tcgggatcat ccagcagagc atggacatgc agatgcggct
    10921 tgccgctcat ttgatacacg ttcgtaatct cctcagctaa tcttagctgt tctacgacat
    10981 gctgcagctt cacccaatcc acttcaatat cgaaatacat cgacaaggtt ttgcccgcct
    11041 tgagcggatt cacgacaatg gtgaaccgat caataatccc atcatccatc aattgatgga
    11101 ttcgttcgcg aacagccgct cgggtcaagt tgaccattcg tccaagctcc gcattgctta
    11161 agcgtccatt ctcggataac agctgcaaaa tccgtgtatc gatctcgtct aaattacgga
    11221 actgcatttg aatcaaccct ttcgactagt acttgtccta ttaggtccat tatagcacat
    11281 acaatgtaaa gttaattgcg aaatctattg atcgattgta taaaaaggtg tatacatatg
    11341 tatgtggaag atcatggcgc ggaaggggag aggatgttgt cattactagc gattgaaata
    11401 ctgttgatct ccgtgttggc tggaatcgtc ggggcagtgc ttggtctggg tggagggatt
    11461 atagttactc ccgcgttgac cttattgttc ggcgttgaca ttaagacggc aattggcgca
    11521 agtattattt cggtcattgc aacctcaagc ggggcagcga tcgcttatat tcgagatcgg
    11581 attacgaatg tgagagtagg catgttcttg gaggttgcaa caacagtcgg agcaataacc
    11641 ggtgcatttc tgggtggaat catggcgcct acggtgctgt atattatctt cggtctgctg
    11701 ctgctctact cggcggggac tatgcttaag aagcggaatg atgaactgcc gaaggatgta
    11761 catatgcatc cggttgcgga gaagctgcgc ttacaaggcg agtattatga taaggcgttg
    11821 aatcagaagg ttccgtataa ggtggacaat gtatacggcg gatttggcgt catgtacggg
    11881 gcaggggtca tatcgggtct gcttggaatt ggcagcggca gcttcaaagt gatggcgatg
    11941 gatgtcttca tgaagc
//
