LOCUS       NZ_LS483389            11894 bp    DNA     circular CON 13-FEB-2024
DEFINITION  Streptococcus pyogenes strain NCTC10879 chromosome 1, complete
            sequence.
ACCESSION   NZ_LS483389
VERSION     NZ_LS483389.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1
  AUTHORS   Doyle,S.
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2018) WTSI, Pathogen Informatics, Wellcome Trust
            Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 01:12:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,835
            Genes (coding)                    :: 1,780
            CDSs (with protein)               :: 1,780
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 36 of 55
            Pseudo Genes (incomplete)         :: 21 of 55
            Pseudo Genes (internal stop)      :: 26 of 55
            Pseudo Genes (multiple problems)  :: 22 of 55
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:51:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1492148
            Orig. end                         :: 1504042
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LS483389.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11894
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11894
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11894
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6894](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6894)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            195..437
                     /locus_tag="DQM81_RS10175"
                     /old_locus_tag="NCTC10879_01552"
     CDS             195..437
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285423.1"
                     /locus_tag="DQM81_RS10175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01552"
                     /product="hypothetical protein"
                     /protein_id="WP_032460069.1"
                     /transl_table=11
                     /translation="MKQQEHTVMMCLNKTLTIKLSAPLTSNDLVPLSTNEVIIMEPLLH
                     LLPKVVPVLIHLVDKANVSWLTVILQLPILLKTIW"
     gene            959..1228
                     /gene="stcA"
                     /locus_tag="DQM81_RS07730"
                     /old_locus_tag="NCTC10879_01553"
     CDS             959..1228
                     /codon_start=1
                     /gene="stcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /locus_tag="DQM81_RS07730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01553"
                     /product="quorum-sensing system protein StcA"
                     /protein_id="WP_023605037.1"
                     /transl_table=11
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTAMTIGHSK
                     QNPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(1576..2007)
                     /locus_tag="DQM81_RS07735"
                     /old_locus_tag="NCTC10879_01554"
     CDS             complement(1576..2007)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017466.1"
                     /locus_tag="DQM81_RS07735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01554"
                     /product="histidine kinase"
                     /protein_id="WP_080290957.1"
                     /transl_table=11
                     /translation="MEVSSEVAVSASQPEELHRLPEEDNNKLSRVAKEVVTEPRLNNPF
                     LLSKKVLSLEDDAAILNHLEEDRNVNKLFLEPVTAEAKREINFHPSDQQVTAKVKESSD
                     FHQKQHQTGITYGDDTLADLTHHLLHSDGVLPSLNDKSA"
     gene            complement(2052..3122)
                     /locus_tag="DQM81_RS07740"
                     /old_locus_tag="NCTC10879_01555"
     CDS             complement(2052..3122)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /locus_tag="DQM81_RS07740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01555"
                     /product="hypothetical protein"
                     /protein_id="WP_038433950.1"
                     /transl_table=11
                     /translation="MMMLRDVKVKTSSLLVGCAATLLVSLSSTVAADSVHSSDRRFGVG
                     YKEETTSQFSSDQPSQVVLYRGEANTVRFTYTNQMYLMKDIRIALDGSGKSLTAQTVPG
                     MGHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMHNTMYKGTVFLV
                     QPQAWKYTMKSVDQLPVDDLNHIGLAGIERMTTLIKNVGALLTTGGSGAFPDNIKVSIN
                     PKGRQATITYGDGSTDIIPPAVLWKKGSVKEPTEADQSVGTPTPGIPGKFKRDQSLNEH
                     EAMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNQDEKEKPASDVKVRPAEVGSWLEPA
                     TALPSVEMSAEDRLKS"
     gene            complement(3373..3774)
                     /locus_tag="DQM81_RS07745"
                     /old_locus_tag="NCTC10879_01556"
     CDS             complement(3373..3774)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /locus_tag="DQM81_RS07745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01556"
                     /product="hypothetical protein"
                     /protein_id="WP_032466306.1"
                     /transl_table=11
                     /translation="MRNILRFLGIVIISAVILFSIGSFYDLPLMKNIQLICWSFILDLF
                     VFVYKQRQTTEVLTWYQVVKQFWLFIKCTILIPTLIAFIIIIGCLTSISEISIYFYLHL
                     TVVCYTIGMILSLGRLISPNHSIFNKLKK"
     gene            complement(3968..4249)
                     /locus_tag="DQM81_RS07750"
                     /old_locus_tag="NCTC10879_01557"
     CDS             complement(3968..4249)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528350.1"
                     /locus_tag="DQM81_RS07750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01557"
                     /product="hypothetical protein"
                     /protein_id="WP_032466308.1"
                     /transl_table=11
                     /translation="MKILDIIFNLSDRKMIFLENVEKLGGYIVPFTLIILSVAYNFRHP
                     YPIWAAVLGGISLIVIFIYFILIVYLYLRYRYLVWKKIPEIKINFTEL"
     gene            complement(4299..4493)
                     /locus_tag="DQM81_RS07755"
                     /old_locus_tag="NCTC10879_01558"
     CDS             complement(4299..4493)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992021.1"
                     /locus_tag="DQM81_RS07755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01558"
                     /product="hypothetical protein"
                     /protein_id="WP_002990768.1"
                     /transl_table=11
                     /translation="MEVLKNGLAIINGGGKDGANIFLTGMAGAAQGVTVCAQTGVFIPW
                     QGYILCGAAGAATNIIWPH"
     gene            complement(4788..4988)
                     /locus_tag="DQM81_RS07765"
                     /old_locus_tag="NCTC10879_01560"
     CDS             complement(4788..4988)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /locus_tag="DQM81_RS07765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01560"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_003060803.1"
                     /transl_table=11
                     /translation="MTTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHPV
                     LTGGCVVGFTVGGAYLGYTAN"
     gene            complement(5001..5228)
                     /locus_tag="DQM81_RS07770"
                     /old_locus_tag="NCTC10879_01561"
     CDS             complement(5001..5228)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016754.1"
                     /locus_tag="DQM81_RS07770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01561"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_038433948.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 4.3e-15,
                     bitscore: 52.6, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIKKLETFHQMTIEKLAKAEGGKNNWQANVSGVIAAGSAGAAIG
                     FPVCGVACGYIGAKTAITFWAGVTGATGGF"
     gene            5546..6232
                     /locus_tag="DQM81_RS07775"
                     /pseudo=""
     CDS             5546..6232
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /locus_tag="DQM81_RS07775"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTQFTTELLNFLAQKQDIDEFFRSSLEIAMNDLLQVELSAFLGYE
                     PYEKEGYNTGNSRNGTYSRQFETKYGLVNLIIPRDRNGEFSPVLLPSYARRDDHLEEMV
                     IKLYQTGVTTREISDIIERMYGHHLFLSGSLVTKRL"
     gene            complement(6452..6634)
                     /locus_tag="DQM81_RS07780"
                     /old_locus_tag="NCTC10879_01564"
     CDS             complement(6452..6634)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987564.1"
                     /locus_tag="DQM81_RS07780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01564"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_111688339.1"
                     /transl_table=11
                     /translation="MDNFLGLQFEELVNISDGKGNIGSAIGRCLGGMLIAAAGGPITGG
                     VAAFVCVASGIAAYQ"
     gene            complement(6649..6894)
                     /locus_tag="DQM81_RS07785"
                     /old_locus_tag="NCTC10879_01565"
     CDS             complement(6649..6894)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994504.1"
                     /locus_tag="DQM81_RS07785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01565"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_038433946.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.6e-14,
                     bitscore: 50.8, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKFAEEIQKEELFHIIGGYSATDCKNHLIGGITSGAIAGGVGAG
                     MAILGVGGVAGVFAGAHVGAIAGGLTCVGGMLFNGK"
     misc_feature    complement(6851..6865)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.12"
                     /tool="antismash"
     gene            7292..7546
                     /locus_tag="DQM81_RS10040"
                     /old_locus_tag="NCTC10879_01566"
     CDS             7292..7546
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990779.1"
                     /locus_tag="DQM81_RS10040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01566"
                     /product="hypothetical protein"
                     /protein_id="WP_010921971.1"
                     /transl_table=11
                     /translation="MSGLFYHKSYFFKSKQVYDLLNEIRMRYPHAHIILIGNHINYEEI
                     FKNHYRVFGVIDTTSHKSLRSIRDQIQLYLDELYNSNNG"
     gene            complement(7852..8328)
                     /locus_tag="DQM81_RS07795"
                     /old_locus_tag="NCTC10879_01567"
     CDS             complement(7852..8328)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /locus_tag="DQM81_RS07795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01567"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_002985741.1"
                     /transl_table=11
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKTW
                     AIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFIYE
                     LTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
     misc_feature    8285..8301
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.42"
                     /tool="antismash"
     gene            complement(8348..9244)
                     /gene="era"
                     /locus_tag="DQM81_RS07800"
                     /old_locus_tag="NCTC10879_01568"
     CDS             complement(8348..9244)
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="era"
                     /gene_functions="other (smcogs) SMCOG1227: ribosome
                     biogenesis GTP-binding protein YsxC"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /locus_tag="DQM81_RS07800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01568"
                     /product="GTPase Era"
                     /protein_id="WP_038433945.1"
                     /transl_table=11
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIMG
                     IYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDDMI
                     IERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTLIKL
                     LTDNLEEGFHYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKRDEET
                     DKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVKVKKNW
                     RDKKLDLADFGYNEKEY"
     misc_feature    9297..9312
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.57"
                     /tool="antismash"
     gene            complement(9364..9771)
                     /locus_tag="DQM81_RS07805"
                     /old_locus_tag="NCTC10879_01569"
     CDS             complement(9364..9771)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /locus_tag="DQM81_RS07805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01569"
                     /product="diacylglycerol kinase"
                     /protein_id="WP_063631267.1"
                     /transl_table=11
                     /translation="MALHDNNTTKRKWKNRTIASSLEFALTGVFTAFKEERNLRSHLLS
                     ACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNAKD
                     MAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            complement(9752..10249)
                     /gene="ybeY"
                     /locus_tag="DQM81_RS07810"
                     /old_locus_tag="NCTC10879_01570"
     CDS             complement(9752..10249)
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ybeY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /locus_tag="DQM81_RS07810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01570"
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="WP_002985748.1"
                     /transl_table=11
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVTN
                     ERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGELFI
                     SIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAYGLT
                     RQ"
     misc_feature    complement(10263..10277)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 18.45"
                     /tool="antismash"
     gene            complement(10408..10983)
                     /locus_tag="DQM81_RS07815"
                     /old_locus_tag="NCTC10879_01571"
     CDS             complement(10408..10983)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /locus_tag="DQM81_RS07815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NCTC10879_01571"
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="WP_009880369.1"
                     /transl_table=11
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIVA
                     QETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFAAK
                     WHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPSPRN
                     QLWLAKNPWFEQDLLPILQKRVEAILTK"
ORIGIN
        1 ctttttacat tgatcatgga tatcctgatt atcgttggtg gataaccttt cgcttaaaaa
       61 caatccactt ttgggaaacc cttatattta gtttgtaaca ggaaaactaa taaatattaa
      121 aaagagtgtt agcgacctag cggcagcttt actcttaagt gcgtttgctg ccattgacag
      181 caaggtcaaa gctgatgaaa cagcaggagc ataccgttat gatgtgtctg aacaaaacac
      241 ttactatcaa actatcagct ccattaacca gcaacgacct ggtgccactc tcgacgaatg
      301 aggtgattat tatggaaccc ttacttcatt tacttcccaa agtcgtacca gtacttatac
      361 acttggtaga caaagccaac gtatcatggt taacagtgat attacagtta cctatactct
      421 taaagacaat ttggtagccc ttcataagga atacaaaacc tttgtcaaaa aaatattaca
      481 gataaaccga tcaccagtga ctacgacaag gctaaggtta tttataacca tttactcaac
      541 gaatacactt acgccacaaa ataacttagc accaatcaca aaaccgtaag cagcatcaac
      601 aatcatactc ctaaagctct ttatcgtggc aagtgtggtg tttgtcaagc ttatgccgtc
      661 atgtttaaag atatggcaga agctgcaggc ttatcagttt ggtatgtgat aaaaaaggat
      721 tgaatgagca ccatgcttga aacatcgtta ccattcatgg tgttaattcc tacgttgatg
      781 ctacctggaa tgattcagca tacaccacaa ggtatttcct tgctggaaaa gacgtgagga
      841 atactgagta ccgtttagat agccagtaca accacttggc taatgctatt ttaaatacca
      901 gctaccaaaa aggctaaact aacaacaact acatttttca actagaaagg cctaaacaat
      961 gaccaaaaaa cagttttatc ttatcttagc tattagcata ctgtgtttta ttcttgccgt
     1021 cagccctaaa ccaccaattc ctaaacacac tattgaaagg aaaaggactg cgatgaccat
     1081 aggccactca aaacaaaacc ctcacaaaaa agaagccctt aaacacaaaa aaaataaaaa
     1141 taaggctcta tttcttccaa gtggggacag cgctatcaaa tctgttatta gcatgacagt
     1201 tagactcctg ttagatgacc aatcttagct ctcctaaaat cagtggacac taccatttcg
     1261 gtagtgttct tttttaagct aatctcatca ccacgctttc ttcaagctaa aaaactgatc
     1321 atgaaagagc aaccccatca ccctaacagc ccactttgcc cgcactttct taaaaagtta
     1381 ggagaaaacg atataccaat tattttctga tcccagctcc ctatcattaa caagatgtgt
     1441 agcacggacc tacatacagg taagctagag tcattctcgc tatttcggac aaaaagaggc
     1501 cggtagttcc ctgacctctt ctttatttat cttgtaacac tcctcttctt agcgagactt
     1561 actagcacat aattcttaag cactcttatc atttagacta ggaagaacac catctgaatg
     1621 aagcaaatga tgcgtcagat ctgccagagt atcatctcca taagtaatcc ctgtttggtg
     1681 ctgtttctga tgaaaatcag atgactcctt aactttagca gtcacctgct ggtcacttgg
     1741 atgaaaatta atttctctct tggcttcagc cgtaactggc tcgaggaaca acttattgac
     1801 attacggtct tcctccaaat gattaaggat agcagcatca tcttctaacg aaagaacctt
     1861 cttggataac aaaaaggggt tatttagcct tggctcagta acgacttcct tggctactcg
     1921 tgatagttta ttattgtctt cctcaggtaa tcgatgcaat tcttccggct gtgaggcgct
     1981 cacagctact tcagaagaaa cctccaagcc ctttcctgtt tgcctgtcta aagcaaccgg
     2041 caaattatta actaactttt taacctgtcc tcagcgctca tttcaacact aggaagagct
     2101 gtcgctggtt ctagccagct accaacttct gctggtctta ccttaacatc gctggcaggc
     2161 ttctccttct catcttgatt aggtctaaaa ggctcaaacc gccctgttga tttttcatct
     2221 atgaccttta tattgtcttt tactacatga gacagcggtt cgacatttac catagcttca
     2281 tgctcgttaa ggctctggtc tcgtttgaat ttaccaggaa taccaggagt cggtgtcccg
     2341 acagattgat cggcttcagt aggctctttt acggagcctt ttttccataa aactgctgga
     2401 ggaataatat ccgtagagcc atccccataa gtaatcgtag cctgcctccc ctttggatta
     2461 atagatactt taatattgtc tgggaaagcc ccactacctc ctgtggttaa aagggcaccc
     2521 acatttttaa tgagagttgt cattcgttca ataccagcaa ggccaatatg gttcaagtca
     2581 tctactggta actgatcaac agatttcatg gtgtatttcc aagcttgcgg ttgaacaaga
     2641 aaaacagttc ctttatacat tgtgttatgc atatcatcaa tgactttgaa ataccttgta
     2701 tatttggttt gtacattagg gttagcaacg ggaacagtgc tttcaggaac tcctgacatc
     2761 gtaaaaatcc ctctagcaga agtttgaaag ccctcataaa catgacccat accaggaaca
     2821 gtctgagcgg tcaaagactt accagaacca tccaaagcaa tgcgaatatc tttcatcaga
     2881 tacatctgat tggtataggt aaacctaacc gtgttagcct cacctcgata caaaaccact
     2941 tgggagggct gatccgaaga aaactgagag gttgtttctt ccttatagcc aactccaaaa
     3001 cgtctatcag acgaatggac tgaatctgcc gcaactgtac tactcaaact gactagtaaa
     3061 gtagccgcac aaccaactaa taaactgctc gttttaactt taacatctct taacatcatc
     3121 atgttccttt cattattttt tatttaggaa ttcgtcatac tatattttct catattagtc
     3181 taacaaaaaa ctcgtaattc tatataaatc atataaaatg acgagctttg gcaaagatga
     3241 gtatagcagg atggaaaaca gattgtttct gaaacttcaa aggtatcttc ttgtcacgaa
     3301 ctattcttta attgaatcgg ttacactaaa ctagtcccat tatttaaaaa caaactttaa
     3361 atatgattca tttcactttt taagtttatt aaatatagag tgattaggag aaataagacg
     3421 acctaatgat aaaatcattc ctattgtata gcaaacaaca gtaaggtgta aataaaagta
     3481 aattgatatc tctgatatac tcgtcaaaca tcctattatt ataataaaag caattaaggt
     3541 aggaataaga atagtacact taataaataa ccaaaattgc ttaacaactt gataccatgt
     3601 gagcacttct gtcgtttgtc tttgtttgta gacaaaaacg aataaatcaa ggataaaact
     3661 ccaacagata agttgtatat ttttcattaa tggtaaatcg tagaaactac ctatactaaa
     3721 tagaatcact gcagaaataa taacaatacc taaaaatctt aatatattac gcatatcttc
     3781 acctcctcta ctctatcggt agcaaatata accgaacaaa ttgttattcc tgttatcaga
     3841 gacagttata acttttttaa ccaattttca atacgcattt tgcttttaac tctttagtct
     3901 ctaaagttaa atatcataag tactgatatt gtataagtca tccattttcc ccttaaatgt
     3961 gattgcatta tagctcagta aaattaatct taatttctgg tatttttttc cataccaagt
     4021 atctataacg caaatataga taaacaataa gtataaaata aataaagatc acaataaggg
     4081 atatacctcc tagtacagca gcccaaatag gataagggtg cctaaagttg taggccacac
     4141 tcaagatgat aagggtaaaa gggacaatgt aacctcccag cttttcaaca ttttctagaa
     4201 atatcatctt tctatcagat aggttaaaaa taatatctaa aattttcata ccactcctct
     4261 actacttata catcagtgat agatagtcat ttatatattt agtgaggcca gataatattt
     4321 gtagcagcac cagctgctcc acacaaaata taaccttgcc aagggataaa tacgcctgtt
     4381 tgagcacaaa ctgtcactcc ctgcgcagca cctgccatcc ctgtcaaaaa gatatttgct
     4441 ccgtcttttc cgcctccatt tatgatagcc agaccatttt ttagcacttc catatttttg
     4501 aagtcttttt cattttaatt tcactctttt gtattttaaa aagctaagag tgatatctaa
     4561 attcattggc ataatcacta ttttctttta tcgataaaaa caaataatga cgctaccaaa
     4621 gctacgatag ataacatgtt taccgatttt tccgaaaggg ataattgatt gaaaattaaa
     4681 aacactaaat atggtataga tttatcaaca ttttttttat gaatggcatc aacaatcatt
     4741 aaaaacatta tagacgcatg cataataata aaaatcgtat agatcattta atttgctgta
     4801 tatcccaaat atgctccacc aactgtaaat cctacaacac atccacctgt caatactgga
     4861 tgtggcagtt tacaaaattt caatccagaa gctaagcctc caagaccacc aattacagca
     4921 tctcctggag cgtttcctcc acttatacta gccatgtcat taattgttaa ttccttcatt
     4981 gttgtcatcc tttctttcct ctagaagccg cctgtagctc ctgttacacc agcccaaaaa
     5041 gtaatagctg ttttagctcc aatataacca caggctacac cgcagactgg aaaacctata
     5101 gcagctccag cactacctgc agcgataacc ccactgacat ttgcctgcca attattctta
     5161 ccaccctcag ccttggcaag cttttctata gtcatttgat gaaatgtttc cagtttcttt
     5221 atctccatat cgaataactc cttttcataa ttcttaaatt tgtctcgttg caacctcgac
     5281 tagtacagaa tatcataaag aacagtatca tggcttcaaa atcttaaaag gccgtttttt
     5341 catcatgaaa tgtcttttta tcctctctac acttacgctg tgacaatagt taattaatta
     5401 agaagtttaa atgataccat tcatgctttt ataaatatct tttaagattt ttgatggtag
     5461 tgtaaaatat cttgtgtaaa cacacaaaaa ggaataaatc ctgtatagta gagttgcgaa
     5521 acttactaga aagagattta ttcccatgac tcagtttacc acagaattac ttaacttcct
     5581 agcacaaaaa caagatattg atgaattttt tcgctcctct ttagaaatag ctatgaatga
     5641 cctcttgcaa gttgaactat cagcttttct tggttatgaa ccttatgaaa aagaaggtta
     5701 caacacaggt aatagccgta atgggactta ttctcgtcag tttgaaacaa agtatggctt
     5761 agtcaattta atcatcccaa gagatcgaaa cggcgagttt tcaccagttt tattaccatc
     5821 ttatgctaga cgagatgacc acttggaaga gatggtgatc aaactctatc agactggcgt
     5881 tacgacacgc gaaatcagtg acattattga gcgcatgtat ggtcaccact tgtttctgag
     5941 tggaagcctc gttacaaaaa ggttatgaca tcacttgaaa caacggaaaa tctcctaact
     6001 ttctatcaat ttccacatta catttggtct agtatttact ctacaaactt gattgaatca
     6061 ctcaataaag aaatcaagcg acaaagcaag aaggagggtg gtttttccaa atgaagaagc
     6121 cttagaacga tgtcttgtaa gtattttcga agactataat attaagttcg gagctcgtat
     6181 tcataaagga ttcggagtat attttgacac acttgatagc ttatttgact aatattcaaa
     6241 aactctactc ttgtgtttac acaacattat tgacagtctc aattaatcta tatcccttta
     6301 atattattaa tttgtaatag tacttagtaa gacataaaca aatatcacaa ttaaaattac
     6361 tttacttgca ttttctctta cctctttatc cttcaaaaaa atttgggaaa gtattagaca
     6421 aattaaaccg agtattataa ctataatcat atcattgata agctgcaatt ccagatgcta
     6481 cacatacaaa tgctgccaca cctccagtta ttggacctcc tgctgcagct ataagcatcc
     6541 cacctaagca ccttccgata gcagaaccaa tatttccttt tccatcagaa atatttacta
     6601 attcttcaaa ctgtaatcct aaaaaattat ccatttttca cctcaaaatt attttccatt
     6661 aaataacata ccaccaacgc aagttaatcc acccgcaata gcacctacat gtgcacctgc
     6721 aaacacacct gcaactcctc caacacctaa aatagccata cctgctccaa caccacctgc
     6781 aatagcacct gaagtaatac caccaattaa atgattttta caatctgtag cactataacc
     6841 tccaataata tggaacaact cttctttttg tatttcttct gcaaatttta tcataacttt
     6901 aaaaaccttt ctaaaatgtt atattcatct tgttgcaact ttgacaaact aaatatagca
     6961 tattattata aaaatttttt tctatgtcgt gaatggttgt ataaacatct caaataaact
     7021 ttttaacata ttagagactg tcaataatgt tgtgtaaaca caagagtaga gtttttgaat
     7081 attagtcaaa taagctatca agtgtgtcaa aacatactcc gaatccttta tgaatacgag
     7141 ctccgaactt aatattatag tcttcgaaaa tacttacaag acatcgttct cttttaggat
     7201 atatgatatc tatctatatt aactattacc tcaagagata ctttctaaaa actcaaatta
     7261 ttagtgggaa aaacttagat tataaataca aatgtcaggt ttattttatc ataaatcata
     7321 cttttttaaa tctaagcaag tatatgacct tctaaacgaa atacgtatgc gttatcctca
     7381 tgctcatatt attctaatcg gaaatcatat taattacgag gaaatattta aaaatcatta
     7441 tcgagttttt ggtgttattg acacaacaag tcataaatca ttaaggtcca ttcgagacca
     7501 aattcaactt tacttagacg aactttacaa ttcgaataat ggataggcac ttcatcaatt
     7561 cttttaggaa atggttcttg tcaccccttc caagtctatt gccacagtta ggttcaactt
     7621 ccctaagtca tgcatggcta gatggcaatt cgtcataact ctaaaacaaa tgtttgatgt
     7681 ctgctagtat caattggaaa gaaaatccta ttaacattta acaaaattat tcagacttaa
     7741 tcagaaaaat tggaagcaaa aaaagagagc ataagctctc gaaacatcgt ctttcaaaaa
     7801 tttttttaac aagccaatct ccaaaaatac tccaactgtc ctagagagtt cttaatgccc
     7861 catagcaaag ctatcactaa aatattcctc taacatcagg cgatgtttgg ctgagacact
     7921 ctctaactct gctatctcct catgagagaa aaactgtaac ttgagcgttt cctcattatg
     7981 aaagttatca atagccatgt cagaaacagc cgttagttca tagataaaga caatggtctg
     8041 aacagcatca ccgttgggat aaacttcctc aaaatgagtg taaacattca gtaagcgaac
     8101 agcttcaacc tcaattccgg tttcctctaa aaactcacgt ttgcaagtct ctaaagagct
     8161 ctcacccagt tccatagtgc caccaggaat agcccaggtt ttcttatcac cacgcaactg
     8221 catcagcacc ttgccgtcat cattggtcaa aatcccacca gcaaagttaa gaataatctt
     8281 atcatgccca actttggagc ggatataaga aatgtagtct tgtggcatat gatctcgctt
     8341 tactatgtta gtattctttc tcattatagc caaaatcagc taaatcaagc tttttatccc
     8401 gccagttttt cttgactttg acccacgttt ctaagtagac cttgtcacct aacatgagtt
     8461 caatatcacg gcgcgccatt tttccgattt ttttgagcat ggctccttgt tttccaatga
     8521 tgatgccttt ttggctatcg cgctccacca taatggttgc acggatatgc actttatcag
     8581 tctcttcatc tcgcttcatg gattcgacaa caacagctac ggaatgtgga acctcttgtt
     8641 gcgtcaaatg cagtaccttc tcacgaacca tttctgacac aaggaaacgt tctggatgat
     8701 cagtaatctg atcttctgga aaatagtgga aaccttcttc aagattatca gttaataact
     8761 tgattagggt tggcacatta ttaccttcta gggcagaaat tgggaccact tctttaaaat
     8821 ccatttggct acggaagtca tcaatctgtt ccaaaagttg gtctggatga accttatcaa
     8881 ttttgttgat gactaaaata acaggaatct tagctgcttt tagccgctcg ataatcatgt
     8941 cgtcaccctt accacgcttt tcatcggcag gaaccatgaa aagaaccgtc tcaacttcac
     9001 gtaaagtact ataagcagat tcaaccataa aatcaccaag ggcggtcttt ggtttatgaa
     9061 tacctggcgt atcaataaag acaatttgtt ctgtttctgt ggtgtaaatg cccataattt
     9121 tattacgggt ggtctgagcc ttgtcactca tgatggcaat tttttgcccc atgacgtgat
     9181 ttaaaaaggt ggatttgcca acatttggac gaccaagaat ggctacaaag cctgatttaa
     9241 acatagataa ttacgggcct taacggtagg gcttcagatc aagcccgtca ctaagcttaa
     9301 ccgtcttgtc ctttccttat tctcctatgc catcatcaca taggatagcc cattcctttc
     9361 tttttaatga acgaaaatat tccaaatttt aggaataaaa ataataagtc ctgtcaagac
     9421 agcatagcca gaaatcatca aaacagcacc tgctgccata tccttggcat ttttagctag
     9481 catagaaaag tgatagtcac tagccaaatc aactacattt tcaatagcag aattgacaat
     9541 ttctaaggta atgaccaaaa aaatggctag aagtaaaaat aaccattcga tggctgaaat
     9601 gctaaaaaat aaaccagcaa cacaagctaa acaggcagac aaaaggtggc ttctgagatt
     9661 gcgttcttcc ttaaaagctg tgaaaacccc tgtcaaggca aactccaagc ttgaggcaat
     9721 ggttctgttt ttccatttcc gtttggtggt attattgtcg tgtaaggcca taagcagtca
     9781 aaatctcttc ctgtaaggta aacatctctt tttcttcttc aagggtgtag tgatcatacc
     9841 cattaatatg taagaatcca tgaacagcta aaaatcccat ttcacgctca aaagagtgcc
     9901 catattcttg ggactgctca cgcgccttgt caatagaaat aaacaattcg ccaatataag
     9961 catcaaactc tgccatcatc tctgccaaac taggatcagc agccaaatcc tcttgactaa
    10021 ataaaatagg cgtttctggc ttgtattcta gagaaatcac atctgttgga cgatcagtat
    10081 ctcggtattc taagttgagt tcatgacttc tttcattggt cacaaaggtc acagacattt
    10141 ctttttcttc tttgcccgtc ttttgagcag caaaattgag caaatcaagt gtttgctcca
    10201 taatctcttg cgaaacttgt cccgtttcgt caatcatctc gatatacata aggttaattg
    10261 ttagggaagg gctgcccacc tcatttggta tgttgggcac ccattattat ctgacagata
    10321 atttccctta tcctttcaat cagcatttgt tatttaccca ttataccaca agccccttaa
    10381 aaatgggaat gctaggtaaa tcagggtcta tttggttaga attgcctcca cacgtttttg
    10441 caagataggt aataaatcct gctcaaacca ggggttctta gctaaccaca attggtttct
    10501 tggagaagga tgaacaagtg gaaaatagtc tggcaagtaa tcctcaaagt gacggactgt
    10561 ttcagttaag gttttgtaag ccttgttacc aaggtaaaag tcctgagcat agcgaccaac
    10621 caaaatcgtc aattcaactt ctggcatcaa tgctcttaat ggcgggtgcc acttagccgc
    10681 aaagccttct ctaggtggta agtcaccgga ttttccctta ccaggataat agaaatccat
    10741 agggataatg ccaaacaatc ctgagtggta aaaggtgtca ttgtccacac ctagccagtc
    10801 tcgtaagcgg atgccactac gatcattcca gtaaagtttg gtttcttgag ctactattcc
    10861 aggagcttgg ccaacaataa taatcctagc tgtttgagga gcatcatata aaggaaaaat
    10921 acctctttcg gtataggaga gattagcctc atctgccata atagctttgg ttaaatcatc
    10981 catagaatcc tccttaaaaa ggccatatta aacatcatcg taaaggtctt agtgctcttg
    11041 accgatcaca gggtattttg tcaagcctgg ctggtaatca gcttcaacgt cactagcctt
    11101 gggttttagt aagtccttcg tctcctctga ggacgtttca taagccttga taatatcagc
    11161 cacaacagga tgtcggacaa catccttagc agagaaatag acaaaatcaa tctgtttaat
    11221 gccttgtaat ttctgagtag catcaatcaa accagacttg acatttcgag ggaggtcaat
    11281 ctgactcgta tctccattga caatcatttt agaattaaaa ccaagacggg tcaaaaacat
    11341 cttcatttgc atgatggtcg tgttttgagc ctcatctaag ataacaaaag cgtcatccag
    11401 agtacgacct cgcatataag ctaacggagc aatctcaatc acatctcgtt ccatcaggcg
    11461 tgtggtttgt tccttgccta aaatgtgata aagggcatcg taaacaggtc tgaggtaagg
    11521 atccaccttt tctttcaaat cgcctggtaa aaaacctagg ctttcgccag cttctaccgc
    11581 aggacgcgtc aaaataatac gctttacctg tcctcttttc agagcagtga ctgcaagcgt
    11641 aacagctaag aaggttttcc ctgtccctgc aggtcctaca ccaaaaacca catcatgacg
    11701 tttgacacta tcaacatagg ttttttgacc taatgttttg actcgtattg ccttaccata
    11761 gttatcttta atgatttctt cttcataaag cgccataaat tggtcaatct gatgagattc
    11821 tgccatagat aaagctgtca ccacatctga tgtgttgacc accatgccac gacccactag
    11881 aaccagtagg gctt
//
