LOCUS       NZ_LR130759            10807 bp    DNA     circular CON 17-DEC-2024
DEFINITION  Mycobacterium basiliense isolate 901379 chromosome I, complete
            sequence.
ACCESSION   NZ_LR130759
VERSION     NZ_LR130759.1
KEYWORDS    RefSeq.
SOURCE      Mycobacterium basiliense
  ORGANISM  Mycobacterium basiliense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5607630)
  AUTHORS   Seth-Smith MB,H. and Seth-Smith,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2018) Functional Genomics Genter Zurich, Division
            of Clinical Microbiology, 4031 Basel, Switzerland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900292015.1-RS_2024_12_16
            Annotation Date                   :: 12/16/2024 14:26:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,815
            CDSs (total)                      :: 4,761
            Genes (coding)                    :: 4,613
            CDSs (with protein)               :: 4,613
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 83 of 148
            Pseudo Genes (incomplete)         :: 100 of 148
            Pseudo Genes (internal stop)      :: 13 of 148
            Pseudo Genes (multiple problems)  :: 47 of 148
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:36:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4886663
            Orig. end                         :: 4897470
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR130759.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10807
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="20"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10807
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10807
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5807](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5807
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(1125..1139)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 22.1"
                     /tool="antismash"
     misc_feature    1126..1140
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: medium, score: 24.07"
                     /tool="antismash"
     gene            1619..2269
                     /locus_tag="MB901379_RS20725"
                     /old_locus_tag="MB901379_04282"
     CDS             1619..2269
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738749.1"
                     /locus_tag="MB901379_RS20725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MB901379_04282"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_158018315.1"
                     /transl_table=11
                     /translation="MSRHWPLFDLRISTPRLQLRLPTEELCDQLIDTILDGVHEPERMP
                     FSVPWTRAPREELPFNTLSHVWQQLARFKTLDWELPLAVVIDGAAVGVQALIAKDFPIT
                     RAVASGSWLGRSYQGRGYGTEMRAAALHFVFTELGAEIATSESMTDNPTSIAVSRRAGY
                     QINGVDRVARDGLMVELLRFRLTRDDWQRHRTVEVQVDGFDQCRGLFGLSRRA"
     misc_feature    1667..1669
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(2281..2628)
                     /locus_tag="MB901379_RS20730"
                     /old_locus_tag="MB901379_04283"
     CDS             complement(2281..2628)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215316.1"
                     /locus_tag="MB901379_RS20730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MB901379_04283"
                     /product="VOC family protein"
                     /protein_id="WP_158018316.1"
                     /transl_table=11
                     /translation="MTLTVEMITFDCHDPATLASWWADQFGGTTRELLPGEFITVARAA
                     GPRLGFQKVPDPTPGKNRVHLDFVAPDVDAEVSRLTAAGATELEQHRFGENFRWVVLAD
                     PEGNVFCVTGQ"
     gene            complement(2641..3936)
                     /locus_tag="MB901379_RS20735"
                     /old_locus_tag="MB901379_04284"
     CDS             complement(2641..3936)
                     /GO_function="GO:0004177 - aminopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396421.1"
                     /locus_tag="MB901379_RS20735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MB901379_04284"
                     /product="M18 family aminopeptidase"
                     /protein_id="WP_158018317.1"
                     /transl_table=11
                     /translation="MSGGPTLSASARGLCEFIDASPSPFHLCATVARRLRNAGYTQLSE
                     TERWPAQPGRYFLVRAGSLVAWNGGGGGDTGGPPGPFRIVGAHSDSPNLRVKQHPDRLV
                     AGWQVVALQPYGGAWLNSWLDRDLGVSGRLSVRDGGQIDHRLVRIDEPVLRVPQLAIHL
                     AEDRKSVTLDPQRHVNAVSGVGDRVGSFLGYVARHAGVIEADVLAADLMTHDLTPSAVI
                     GANADLVSAPRLDNQASCYAGLEALLGATPGEVLPILVIFDHEEVGSASDRGAQSNLLS
                     TVLERIVLAAGGGREDLLRLLPASLLASADMAHATHPNYPDRHEPGHLIEVNKGPVLKV
                     HPNLRYATDGPTAAAFAVACQQAGVTLQRYEHRADLPCGSTIGPLASARTGIPTVDVGA
                     PQLAMHSARELMGAHDVVAYAAALHAFFSPTA"
     gene            3946..5004
                     /locus_tag="MB901379_RS20740"
                     /old_locus_tag="MB901379_04285"
     CDS             3946..5004
                     /EC_number="1.11.1.-"
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877197.1"
                     /locus_tag="MB901379_RS20740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MB901379_04285"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_162334422.1"
                     /transl_table=11
                     /translation="MAVEDYVVHARVGRVPSVQPQPILAPLTPAAIFLVATIDDGGEAT
                     VHDALADISGLVRAVGFRDPSKRLSVVASIGSDAWDRLFSGPRPAELHPFIALEGPRHT
                     APATPGDLLFHIRAETMDVCFELAGRILKAMAGAVTVVDEVHGFRFFDNRDLLGFVDGT
                     ENPGGPVAVSATTVGDEDPDFSGSCYVHVQKYLHEMQSWDALSVTEQERVIGRTKLEDI
                     ELDDAAKPANSHIALNVITDDDGAELKIVRHNMPFGEVGKGEYGTYFIGYSRTPQVTER
                     MLRNMFLGDPPGNTDRVLDFSTAVTGGLFFSPTITFLDDPPPLPVAPPTPAVTPASNDG
                     SLSIGSLKGTSQ"
     misc_feature    3957..3971
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.43"
                     /tool="antismash"
     gene            5001..5807
                     /locus_tag="MB901379_RS20745"
                     /old_locus_tag="MB901379_04286"
     CDS             5001..5807
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738747.1"
                     /locus_tag="MB901379_RS20745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MB901379_04286"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_158018319.1"
                     /sec_met_domain="Linocin_M18 (E-value: 2e-94, bitscore:
                     313.6, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLYRELAPVTEAAWAEIELEATRTFKRHIAGRRVVDVGGPGGP
                     VTAAVSTGRLIDVQAPTDGVIAHLRASKPLVRLRVPFTLSRAEIDDVERGSQDSDWDPV
                     KVAAKKLAFVEDRTIFEGYAAASIEGIRSASSNPPLTLPEDPREIPDVITQALSELRLA
                     GVDGPYSVLLAADVYTKVSETTEHGYPIREHLNRLVDGDIIWAPAIDGAFVLTTRGGDF
                     DLQLGTDVAIGYTSHDADTVQLYLQETLTFLCYTAEASVALSPPSQ"
     gene            complement(5943..7814)
                     /locus_tag="MB901379_RS20750"
                     /old_locus_tag="MB901379_04287"
     CDS             complement(5943..7814)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1142: PPE family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177817.1"
                     /locus_tag="MB901379_RS20750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MB901379_04287"
                     /product="PPE family protein"
                     /protein_id="WP_158018320.1"
                     /transl_table=11
                     /translation="MLPNFALLPPETNSALMFAGSGSAPWWTAAGAWDALAEELRSAAA
                     SFSSVTEGLTAGLWQGAASAAMAGAAAPYAAWLNASAARAQGAAGQARAVAGVFEAARA
                     AIVNPAVIAANRTQIVSLVLSNVFGQNAPAIAAVEGAYEEMWAQDVSVMAGYHGGASAA
                     AAALRSWQQSLREALGFSLPQNAGLGNNGTGNVGNGNHGDSNLGNGNLGSANVGSGNTG
                     NSNLGSGNIGSSNLGGGNRGESNVGGGNSGHGNLGGGNSGSGNVGFGNNGDGNVGGGNK
                     GHGNFGFGNVGPTETDALSNIGSGNTGSFNRGSGNTGDSNWGFGNTGDGNIGVGNSGNG
                     NIGFGLTGNNQMGIGALNTGSGNIGFGNSGNGNIGFFNSGTNNVGVFNSGFANTGFEIS
                     GTNNTGFQTTGGTNTGFWNTGLEATGFGNTSGETTGAFNSGRYTTGFFNSGDANSGWFN
                     SGKGNTGSANSGDGNTGWFNAGATNTGFGNSGNINTGIFNSGNLNTGIGSVGNGPGTSS
                     GFGNLGTGASGFFNQGNNASGFVNSGDDTSGARNSGPDSSGFNNSGFGGSGFQNSSDRG
                     SGFFNSVNDGVGFQNSGFFNTGIRNSGFGNVSEFPGTDSGHSGFFHR"
     gene            8180..8680
                     /locus_tag="MB901379_RS20755"
                     /old_locus_tag="MB901379_04288"
     CDS             8180..8680
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009479845.1"
                     /locus_tag="MB901379_RS20755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MB901379_04288"
                     /product="cupin domain-containing protein"
                     /protein_id="WP_158018321.1"
                     /transl_table=11
                     /translation="MTFSDDFHPDFSSDASTPGARPMRVHHIKATEISSDTAQSEGLRR
                     FAALSGRSVGSEKLWMGETHVSPETSSANHHHGESETAIYVRSGRPEFVFHDGVQEVRI
                     STQPGDYIFIPPYLPHREENPDPVAPAEVVIARSTQEAIVVNLPQLYPLEASTRPGADP
                     APA"
     gene            8810..9265
                     /locus_tag="MB901379_RS20760"
                     /old_locus_tag="MB901379_04289"
     CDS             8810..9265
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730751.1"
                     /locus_tag="MB901379_RS20760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MB901379_04289"
                     /product="YbjN domain-containing protein"
                     /protein_id="WP_158018322.1"
                     /transl_table=11
                     /translation="MTESLDTKLIERYLRARGRRYFRGQHDGEFFFVMNVVRLHVHLEI
                     PVTDREIFTIRVTPACFFPAADAPTLRQVVDTWNDQCREVTATLHGSSDPHRIGVAAIV
                     SQPVENGVAFEDFAMFVDHAIAAAIEFFDGLTPIAELSSTPLLRDAG"
     gene            complement(9343..10017)
                     /gene="purQ"
                     /locus_tag="MB901379_RS20765"
                     /old_locus_tag="MB901379_04290"
     CDS             complement(9343..10017)
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /GO_function="GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="purQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738743.1"
                     /locus_tag="MB901379_RS20765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MB901379_04290"
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="WP_158018323.1"
                     /transl_table=11
                     /translation="MTVRIGVVTFPGTLDDVDAARAVRQVGADAVSLWHADADLKRVDA
                     VVVPGGFSYGDYLRAGAIARFAPVMGEVVAAAGRGMPVLGICNGFQVLCEAGLLPGALT
                     RNVGLHFICRDVWLRVGSTLTAWTSRFEPDADLLVPLKSGEGRYVAPASVLDQLEGDGR
                     VVFRYHENVNGSLHDIAGVSSPNGRVVGLMPHPEHAIGALTGPSDDGLGLFYSALDAVL
                     TA"
     gene            complement(10014..10253)
                     /gene="purS"
                     /locus_tag="MB901379_RS20770"
                     /old_locus_tag="MB901379_04291"
     CDS             complement(10014..10253)
                     /EC_number="6.3.5.3"
                     /codon_start=1
                     /gene="purS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177755.1"
                     /locus_tag="MB901379_RS20770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MB901379_04291"
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="WP_158018324.1"
                     /transl_table=11
                     /translation="MARVVVHVMPKAEILDPQGQAIVGALGRLGHPGVSDVRQGKRFEL
                     EVDDTVDDSTLAEIAESLLANTVIEDWTISRDQQ"
     misc_feature    complement(10167..10169)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 caccgcgacc ggcgtggcgc cggtgacggc aacattgcga tacgcctcgg ccaatgccag
       61 ctgggctccc gcgtaggggt ccagcaacgt gtagcgcccg gacgcgtcgg tggacaacgc
      121 gatgccccgt ccggtcgact cgtcgatgcg caggacgccg ccgtcggcgt gctcggccag
      181 cacggtgttg ccgcgcacat agcggtcgta ctgctcggtg atgaatgcgc gactgcacag
      241 gtgcggacta ctgagtaacg caagcaaagt cgcgcgcagc tcgtccccgg aagagggccg
      301 aggcaatccg gtcgaggtgt cggcgttgag tgcatcttgt gcgtcggggc gggcaaccgg
      361 gcgctggtac accggcccct cgtgggccac tgtgcgcggc ggcacatcga cgaccgtctg
      421 accctgccag ctgatctgca gccggtcgcc gtcggtgact tcaccgatca ccgtggccag
      481 cacatcccac ttgcgacaca ccgccatgaa ggcattcaca ttctccgggg cgaccaccgc
      541 acacatccgc tcctgcgact cgctgcagag cacttcggct ggcgtcatat ccttggcccg
      601 cagcgggacg gtatcgagtt ggatcgccat tccgccgtcc ccggcagagg ctaactccga
      661 tgtggcacag gataatccgg ccccacccag atcctgaatg ccgatcacca gtccgctcgc
      721 gtagagctcg aggcaacact cgatcagcac tttctccatg aacgggtcgc ccacctgcac
      781 cgagggcaat ttcttgcgtg agcctgcact gtcgaaggtg tccgatgcca ggaccgacac
      841 cccgccgata ccatcgagtc ccgttcgcgc gccgaacaga atgatcttgt tcccggcacc
      901 cgaagcgaac gccagatgca gatcctcctg ccgcaatacg ccgacacaca acgcgttgac
      961 caacgggttt ccggcataac acgcatcgaa gacggtctcg ccaccgatat tgggcaaacc
     1021 caacgaattg ccgtagccac ccacaccgcg aaccacgccg tcgagcaccc ggcgggtatc
     1081 tggcgcctcg gcagggccga atcgcagctg gtccatcacc gcgaccgggc gcgctcccat
     1141 ggccatgatg tcgcgcacga tgccaccgac accggtggca gcaccctggt aaggctctac
     1201 ataggacggg tggttgtgcg attccacctt gaacgtgacg gcccagccgt cgccgatgtc
     1261 gaccacgcct gcgttctcgc cgattccggc cagcatgccc gcgcgcatct gctcgctggt
     1321 ggtctcaccg aaatagcgca ggtgaacctt ggatgacttg taggagcagt gctcactcca
     1381 catcaccgag tacatggcca gctcggtatc ggtggggcgg cgccccagga tgtcacgaat
     1441 ccgctggtac tcgtcgtctt tgaggcccag ctcatggaat ggttgcgggt ggtcgggggt
     1501 ggtggcggca tgttcgacgg tgtcaatcac ctgggtgcgc gcggtcgtca cggggacagt
     1561 ctaggttccc cggggaaatc cagctgacag cgcttgggac gatccagata atgatcggat
     1621 gtctcgtcat tggcccctgt tcgacctgcg gatcagcacg ccgcgcttac agctgcggct
     1681 gcccaccgag gagctgtgtg accagctgat cgacaccatt ctcgacgggg tgcacgaacc
     1741 cgagcggatg cctttctcgg tcccttggac cagagctcct cgcgaagagc tgccgttcaa
     1801 cacgctgtcg cacgtatggc aacagctcgc ccgcttcaaa acgctggact gggaattgcc
     1861 gctggccgtc gtgatcgacg gcgcggcggt cggcgtacag gccctgatcg ccaaggattt
     1921 ccccatcacc cgcgcggttg catccggatc gtggctcgga cgaagctacc aaggccgcgg
     1981 ctacggcacc gagatgcgag ccgcggcact acatttcgtg ttcactgagc tcggcgccga
     2041 aatcgcgact tcggaatcga tgaccgacaa cccgacctcc atcgccgtat cgcgccgcgc
     2101 cggttaccaa atcaacggcg tggaccgcgt ggcgcgtgac ggcctcatgg ttgagctact
     2161 gcgtttccgg ttgacccggg acgattggca gcgccatcgc acggttgagg tgcaagtgga
     2221 tgggttcgac caatgccgcg ggctgttcgg actttcgcgc cgagcgtgac gaggggctag
     2281 ctactgcccc gtcacgcaga acacgttgcc ctcggggtcg gccagcacca cccagcgaaa
     2341 gttctccccg aaccggtgct gttcaagttc ggtggcgccc gcggccgtca accgggatac
     2401 ttcggcatcc acatctggag caacgaaatc gaggtggacg cggtttttcc ccggcgtggg
     2461 atcaggaacc ttctgaaatc cgagccgcgg gcccgcggct cgggccaccg taatgaattc
     2521 accgggcaac aattcgcgcg tagtaccgcc gaactggtcg gcccaccagc tggccaacgt
     2581 cgcggggtcg tggcaatcga atgtgatcat ctccacggtg agcgtcatag cctgtagacc
     2641 ctacgcagtg ggggaaaaga acgcgtgcag cgccgccgcg taggcgacca catcatgagc
     2701 acccatcagc tcccgcgccg aatgcatcgc aagctggggg gcgcccacgt cgacggtggg
     2761 gatgccggtg cgggccgaag ccagcgggcc gatcgtcgac ccacacggca ggtcggctcg
     2821 atgctcgtag cgctgcagcg taaccccggc ctgctggcag gccactgcga acgccgcggc
     2881 ggtgggcccg tcagtggcgt accgcaggtt cgggtgcacc ttgagcaccg gccccttgtt
     2941 cacctcgatc aggtggcccg gctcatgacg atcggggtag ttggggtgtg tggcgtgcgc
     3001 catgtccgcc gacgccaaca acgaggcggg cagcaacctt agcaggtctt cgcggccacc
     3061 gccggcggcc agcacgatcc gttccaggac ggtgctcagc aggttggact gggcgccgcg
     3121 atcggaggcc gaaccgacct cctcgtggtc gaagatcacc aggatgggca acacctcacc
     3181 cggggtggcg cccagcagcg cctccagtcc ggcatagcaa ctggcctggt tgtcgagccg
     3241 cggcgcgctt accaggtcgg cgttggcgcc gatcaccgcc gacggcgtca ggtcgtgggt
     3301 catcaggtcg gcggccagca cgtcggcctc gatcaccccg gcatgccgcg caacgtagcc
     3361 gaggaacgag ccgacgcgat cgcccacgcc cgacaccgcg ttgacgtgcc gctgtggatc
     3421 cagcgtcacc gacttgcggt cctcggccag gtggatggcc agctgcggca cccgcaggac
     3481 cggctcatcg atgcggacca gccggtgatc gatttggccg ccgtctcgca ccgaaagccg
     3541 cccgctgaca cccagatcac ggtccaacca ggaattgagc cacgcgcccc cgtatggctg
     3601 tagcgcgacc acctgccaac cggcgaccag ccggtcgggg tgctgtttga cccgtaggtt
     3661 cgggctgtcg ctatgtgcgc cgacaatccg gaatgggcct ggcgggccac ccgtatcacc
     3721 gccgccacca ccgttccagg cgaccagcga accggcacgc accaggaagt aacgcccggg
     3781 ttgcgctggc cagcgctcgg tttcgctgag ttgggtgtac ccggcgttac gcagccgtcg
     3841 ggcgacggtg gcgcacaggt gaaaaggcga cggtgaggca tcgatgaact cgcagaggcc
     3901 acgcgcgctg gctgagaggg taggcccgcc ggacatgtga ccatcatggc tgttgaggac
     3961 tatgtggttc acgctagggt cggcagggtg ccttcggttc agccacaacc catcctcgca
     4021 cccctgacgc ccgccgcaat ctttctcgtc gcaaccatcg acgacggcgg cgaagcgacc
     4081 gtgcacgacg cgcttgccga catatccggt ttggtgcgtg cggtgggctt tcgcgatccg
     4141 agtaaacggc tgtcggtcgt cgcctcgatc ggttccgatg cctgggaccg gttgttttcc
     4201 ggcccgcgcc cggccgaact gcatccgttc atcgccttgg agggaccgcg gcacacggcg
     4261 ccggcaacac ccggtgatct gttgttccat atccgagccg aaaccatgga cgtgtgcttc
     4321 gaactagccg gccgcattct caaagccatg gccggtgccg tcaccgtggt cgacgaggtg
     4381 cacggattcc gcttcttcga caatcgcgac ctgttgggct ttgtggacgg caccgaaaat
     4441 ccaggtggcc cagtggctgt cagcgccacc accgtcggcg acgaggaccc cgacttttcc
     4501 ggatcctgtt atgtgcatgt gcagaagtat ctgcacgaga tgcagtcctg ggatgcgctg
     4561 tcggtcaccg agcaggaacg ggtgatcggg cggaccaagc tggaagacat cgaactcgac
     4621 gacgcagcga aacccgccaa ctcgcatatc gcgctgaatg tcatcaccga cgacgacggc
     4681 gccgaactaa aaatcgtgcg tcacaatatg ccgttcggcg aggtcggaaa gggcgaatac
     4741 ggcacctact tcatcggcta ttcgcgcacg ccccaggtca ccgagcggat gctgcgcaac
     4801 atgtttctcg gtgatcctcc cggcaacacc gatcgcgtgc tggacttttc gacggcagta
     4861 accggtggat tgttcttctc ccccaccatc accttcctcg acgatccacc gcctctgccc
     4921 gtcgcaccgc ccaccccggc ggtcacccca gcctcgaatg acggttcact ctcgatcggc
     4981 agcctgaaag gaacctctca atgaacaacc tctaccgcga actggcaccc gtcaccgaag
     5041 ctgcctgggc cgaaatcgaa ttggaggcga cgcggacgtt caaacggcac atcgccgggc
     5101 gtcgggtggt tgacgtcggc gggcccggcg gtccggtcac cgcagcagtc agtacgggcc
     5161 ggctgattga cgtccaggcc cccaccgacg gcgtcatcgc ccacctacgc gcaagcaaac
     5221 cccttgtccg gctgcgcgtt ccgtttaccc tgtcgcgtgc cgagatcgac gacgtcgaac
     5281 gcggttcgca ggactcggac tgggatccgg tcaaagtggc cgccaagaag ttggcgttcg
     5341 tcgaggaccg caccatcttc gagggctatg ccgccgcgtc gatcgagggc atccgcagcg
     5401 ccagctccaa cccgccgctc acgttgcccg aagacccgcg cgaaattccc gatgtcatta
     5461 cccaggcgct atcggagctg cgcctggccg gtgtcgatgg cccgtactcg gtgctgctcg
     5521 cggccgacgt ttacaccaag gtcagcgaaa ccaccgaaca cggctatccc atccgcgagc
     5581 atctgaaccg cctggtcgac ggcgacatca tctgggcgcc ggcgatcgac ggcgcattcg
     5641 tgttgaccac ccgcggcggc gactttgacc tacagttggg caccgacgtc gcgatcgggt
     5701 acaccagcca cgacgccgac accgtgcagc tctacctaca ggaaacgctg accttcctgt
     5761 gctacacagc cgaggcatcg gtcgcgctga gcccgccaag ccagtagcac gctaaaggtc
     5821 gagaaccaga tcggtttgag gagctgccga gcagatgagt acgtctttct ggggtggtcc
     5881 tcccggccac tcttcccata acacacaggt ggcacacgcc agccaatgcg ctggcgccga
     5941 aatcagcggt gaaagaagcc cgagtgcccg ctgtcggttc ccgggaattc actcacgttc
     6001 ccaaaacccg agttgcgaat gccggtgttg aaaaagcctg agttctggaa gcccacgcca
     6061 tcgttgacgc tgttgaagaa gcccgaaccc cgatcactcg agttctggaa gccagagccg
     6121 ccaaatcccg agttgttgaa gcccgaacta tcgggcccgg agttgcgcgc gcccgaggta
     6181 tcgtcgccgg agttgacgaa gccggaggca ttgttgccct ggttgaagaa gcctgaggca
     6241 ccggtaccca ggttcccgaa tcccgagctg gtgccgggcc cgttgccgac gcttccgatg
     6301 ccggtgttca ggttgcccga attgaagatg ccggtgttga tgttgcccga gttgccgaac
     6361 ccggtgttgg tcgcaccggc gttgaaccag ccggtgttcc cgtcgccgga gttggcggat
     6421 ccggtgttgc ccttccccga attgaaccag ccgctattcg cgtcgcccga gttgaagaat
     6481 ccggtggtat agcggcccga gttgaacgca cctgtcgttt cgccggaggt gttcccgaaa
     6541 ccggtggctt ccagaccggt gttccagaag ccggtattgg tgccgccggt ggtctgaaac
     6601 ccggtgttgt tcgtcccgga aatctcaaac ccggtgttgg caaagcccga gttgaagacg
     6661 ccgacattgt tcgtgccgga gttgaagaat ccgatgttgc cgttgcccga attgccgaac
     6721 ccgatgttgc cgctgccggt gttcaaggcg ccgatgccca tctgattgtt acccgtgagg
     6781 ccgaacccga tgttcccgtt accgctattt ccgacgccga tattgccgtc gccggtgttg
     6841 ccgaagcccc agttggaatc gccggtattc cccgaacccc ggttgaagct tccggtgttt
     6901 ccgctgccga tgttggacag ggcatcggtt tcggtgggac cgacgtttcc gaatccgaag
     6961 tttccgtggc ccttgttccc accgccgacg ttgccatcgc cattgttgcc gaagcccacg
     7021 ttcccgctgc cgctgttgcc gccgccgagg ttgccgtgac cggagttacc gccgcccaca
     7081 ttcgattcgc cgcgattccc gccgcccagg ttggaactac cgatgtttcc gctccccaag
     7141 ttggagttgc cggtatttcc gctgcccaca ttggcgcttc ccaggttccc gttgcccagg
     7201 tttgagtcgc catggtttcc gttaccgacg ttgccggtgc cgttgttgcc gagaccggcg
     7261 ttttgcggca gggagaaacc gagcgcttcc cgcagtgatt gctgccagga cctcagcgcc
     7321 gcggccgccg cggaggcacc gccatggtag ccggccatca cgctgacgtc ctgggcccac
     7381 atctcttcgt aggcgccctc gacggccgcg atggccggag cgttttgacc gaacacgttc
     7441 gacagcacca aggacacgat ctgggttcgg ttggcggcga ttaccgcggg attcacgatc
     7501 gcagcgcgag cggcctcgaa caccccggcg accgctcggg cctgacccgc cgccccctga
     7561 gcccgcgccg ccgaggcgtt cagccatgcc gcgtatgggg cggccgcgcc ggccatcgcc
     7621 gcggatgccg caccctgcca caacccggcg gtcaaaccct cggtcaccga cgagaacgag
     7681 gcggccgccg agcgcaactc ctcggccaac gcgtcccacg cacccgccgc cgtccaccag
     7741 ggcgccgacc ctgagccggc aaacatcaac gcggaatttg tttccggcgg cagcaatgcg
     7801 aaattcggca gcatcgtgac tcctcatacc caccaccaca gcgcaatgtt tgccactggt
     7861 ttcagcaaaa cctagcccca tcggcgtcaa ttcgtgaccg aatctgtaat tcgtcttcgc
     7921 tgcacttcgg aggcgtagcc tcgcaccaga gccgaggaag gaggccgagc atgccgacca
     7981 ccatcccgcg tcgcctcaga gccgcgaagc cgcgtctgtc gtatggtcgg tccgggtacc
     8041 gtcgccgtga cctgtcagga gcccatgatc gccgccggca gggcgcacct cgatctccca
     8101 ctgtcaccga actgcgactg aaaatgcttg cagcataacg tgatacgctt ttcactagcg
     8161 tccaacaatg ggagcccaga tgacattttc ggatgacttt caccctgatt tttcttccga
     8221 cgcttcgact ccaggcgctc gcccgatgcg ggtgcatcac atcaaagcga cggaaatcag
     8281 ctccgacaca gcgcaatcgg agggactgcg tcgattcgcc gccctcagcg ggcgctcggt
     8341 cggctccgag aagctctgga tgggcgaaac ccatgtttcg cccgaaacct cctcggccaa
     8401 tcaccatcac ggcgagtccg agactgcgat ctatgtacga agcggccggc ctgagttcgt
     8461 gtttcacgac ggcgtccaag aagtgcggat ctcgactcaa cccggcgact acatcttcat
     8521 acctccctac ctgccacatc gcgaagaaaa ccccgacccc gtcgcacccg ccgaggtcgt
     8581 catagcccgc agcacccaag aggcgatcgt ggtcaacctg ccgcagctgt atccgctcga
     8641 agcgtcgacg cgccccggtg cagacccggc ccctgcctaa cggcgctgca aaagcccagt
     8701 tcgagcccgg cgttcggtgg tttgcctcag gcgattctca gctgattctt cgctatcgct
     8761 ttccctggtt ccaaggatcc ctataggcgc cggcagcacc atggattcca tgaccgagtc
     8821 gctggatacc aaactgatcg aacgctacct gcgtgcgcgc gggcgtcggt acttccgtgg
     8881 ccagcacgat ggcgaatttt ttttcgtcat gaacgtggtt cgactgcacg tgcaccttga
     8941 aatcccggtc accgatcgcg agattttcac gattcgggtc acacccgcct gtttcttccc
     9001 cgccgccgac gcgccaacgc tgaggcaggt cgttgacacc tggaacgacc agtgccgcga
     9061 agtcaccgcg accctgcacg gctcgtctga tccgcaccgg attggtgtcg ccgcgatcgt
     9121 ttcccagccg gtggaaaatg gtgtggcgtt cgaggatttc gcaatgtttg ttgaccatgc
     9181 cattgcggcc gcgatcgagt ttttcgatgg gttgaccccc atcgccgagt tgtcgtcgac
     9241 accgctgcta cgcgacgccg gctgaggggc gcggccggct tgcccctcac tcgacgcaaa
     9301 attcgacgaa accccgcggg gccgtagatc gtctgctcgg cctcaggccg tgagcacagc
     9361 gtccagcgcc gaatagaaca accccagtcc atcatcggac gggccggtca gcgccccgat
     9421 cgcatgttcg gggtggggca tcaacccgac cacacgaccg ttaggcgagc tgacgccagc
     9481 gatgtcgtgc agcgagccgt tgacgttctc gtggtatcgg aacaccaccc gcccgtcccc
     9541 ttcgagctga tcgagcacgg acgctggcgc cacgtagcgt ccctcgccgg acttcaacgg
     9601 caccagcaag tccgcatcgg gctcgaaccg cgatgtccag gccgtcaacg tcgacccgac
     9661 ccgtagccag acgtcacggc agatgaagtg caaccccaca ttgcgggtca gggctccggg
     9721 tagtagaccg gcctcgcaga gcacctgaaa cccgttgcag atgcccagca ccggcattcc
     9781 gcggccggcg gcggcaacca cctcccccat taccggggcg aatctagcga tcgccccagc
     9841 ccggaggtag tcaccgtagg aaaaaccacc cggaaccacc accgcgtcga cccgcttgag
     9901 gtcggcatcg gcatgccaca ggctgaccgc atcggcaccg acttgccgca ccgcacgcgc
     9961 ggcgtccacg tcatcaagcg tgccggggaa ggtgacgacg ccgatcctca ccgtcactgc
    10021 tgatcccggc ttatcgtcca atcctcgatt accgtgttcg ccagcagcga ttccgcgatc
    10081 tcggcgaggg tggaatcgtc gacggtatcg tcaacctcca gctcaaacct cttgccctga
    10141 cggacatccg agacgccggg atgccctaac ctccccagtg ccccgacgat cgcctgcccc
    10201 tgcggatcga gaatctctgc tttgggcatc acgtgcacga ctacccgggc cacccgcgct
    10261 cctcctcaga tccgattgct cggcgccaac tctaccggcg accgtggccg ccaccaacgc
    10321 ttaggggcag gaggccatgt aggccgcaca cagcggcgtg gtcatggcgt ccagaacggc
    10381 ggcgtccgcg atcaccggcc actccacctg cggtgctccc gctgtccaca gcgtgagctc
    10441 gctgatcgtg ctactcgcca gatgtgcgac aaaataggta tgcatgatga tcgctccgct
    10501 gatcaccgcc gccattcgag tcggttcgag gtcggtgatc gacggcgact ggccaggcgc
    10561 ccccagctgg catgcgcgca gcgtggcgac cgcggtgccg aacaccgacg ccgcaatggc
    10621 accgccgcgg gcggtgtcac ctcgccagtg caggatctga gcgtgtagct gccactgccc
    10681 gtccgggtgg gccaccgccg cgcggcccgc gaccgccgag tcacggctgt cctgcgggaa
    10741 agctggcgtg ttacatgctt gctcgaaccg gaaccgcggt gttgtcctgg tgacagcggt
    10801 tgccgcg
//
