LOCUS       NZ_LT963352            10495 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Streptomyces chartreusis NRRL 3882 isolate NRRL3882 chromosome I.
ACCESSION   NZ_LT963352
VERSION     NZ_LT963352.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces chartreusis NRRL 3882
  ORGANISM  Streptomyces chartreusis NRRL 3882
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wibberg,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2017) CEBITEC, Genome Research of Industrial
            Microorganisms (GRIM), Universitaetsstrasse 27, 33615, NRW, Germany
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:03:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,073
            CDSs (total)                      :: 7,984
            Genes (coding)                    :: 7,779
            CDSs (with protein)               :: 7,779
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 91 of 205
            Pseudo Genes (incomplete)         :: 161 of 205
            Pseudo Genes (internal stop)      :: 14 of 205
            Pseudo Genes (multiple problems)  :: 56 of 205
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:02:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5834948
            Orig. end                         :: 5845443
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT963352.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10495
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="20"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10495
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10495
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5495](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5495
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(558..1799)
                     /locus_tag="SCNRRL3882_RS26425"
                     /old_locus_tag="SCNRRL3882_5192"
     CDS             complement(558..1799)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003990352.1"
                     /locus_tag="SCNRRL3882_RS26425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_5192"
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="WP_010046802.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 1.1e-33,
                     bitscore: 115.1, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHTAPLPRVLHLTQPVDGGVARVVTDLVRAQLSDGLDVTVACPDS
                     ALTTKLRTLGARVRHWHATRSPGTSLVREVRHLARVIDEVRPDLVHAHSAKAGLAGRLA
                     VRGRIPTVFQPHAWSFEAVSGGTAALALLWERWGARWASRVVCVSEAERATGMDARITG
                     RWTVVPNGIDPERFRPAPAGAVRAGLAPLAGIRPAAPLAVCVGRLCRQKGQDVLLRAWE
                     AVVRQVPDARLVLVGDGPDHDRLREGAPESVVFTGAVADASPWYQASDLVVLPSRWEGM
                     ALAPLEAMACGRPVVVTDVDGARESLPPSFAARCLVPPEDPAALAGAVTELLADPLLRE
                     SLGSQGRRHVLSTHDVRYTAEAVSDVYRDLLGPWTAATPSAEHGQPLGTGRGAEANRTP
                     QRVEPTEYRESIHS"
     gene            complement(1850..2938)
                     /locus_tag="SCNRRL3882_RS26430"
                     /old_locus_tag="SCNRRL3882_5193"
     CDS             complement(1850..2938)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056483.1"
                     /locus_tag="SCNRRL3882_RS26430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_5193"
                     /product="hypothetical protein"
                     /protein_id="WP_010046798.1"
                     /transl_table=11
                     /translation="MRHSLGPVFRRATVGVLALAAVWAASSSPAAAPAAAPAPEAESLG
                     FAERYRTLQHGGIVRAANASITCRTAQAPSCPDAQAGGSGVNGDFDMFYVDVDRDPNTY
                     NSSRAEVRLPNASRVTYARLYWGGNLRVGEQKPPQDNERVLIAEPGGEYKELLADTTVG
                     HRVADGMDAFQASADVTRLVREGGPGLYTVAQINIAMGRSAAGAWGGWTLVVAYENPSE
                     PLRHLALWDGFTPLRPGAAQEIRMTDLGFAAGAWGRAGLVTYNGDRGTTGDSLTVTTGQ
                     SEFALTNAANPRDDVLNSTISEPGADAARVPAHANNLGYDSDVFDLGSGLQQAGDQATF
                     RLHSQQDAAWAGVLFAAVDARR"
     gene            3341..4021
                     /locus_tag="SCNRRL3882_RS26435"
                     /old_locus_tag="SCNRRL3882_5194"
     CDS             3341..4021
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SCNRRL3882_RS26435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SCNRRL3882_5194"
                     /product="hypothetical protein"
                     /protein_id="WP_029181652.1"
                     /transl_table=11
                     /translation="MCALPARRIASSALCAALLVGITGPVAMAADSARGHAHVAPDARL
                     PGADARLAQIDRLNWGELTPVADLLHAVLRSDGRLPASEARKLGAEAKAALTEAAAKGE
                     RTSMTSTVVLLPAPALPALVPGAPDRRAADPVTDLLDLVLGAVDSLLQAITSGVGGILP
                     SVDDLLNGVDDLLAGLIGGNLLQDDSVSSPSSSTSSTSVPTTMPAAVTYPAVPLLTRVV
                     PPAS"
     misc_feature    4129..4145
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.39"
                     /tool="antismash"
     misc_feature    4697..4711
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.3"
                     /tool="antismash"
     gene            5001..5495
                     /locus_tag="SCNRRL3882_RS26440"
                     /old_locus_tag="SCNRRL3882_5195"
     CDS             5001..5495
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826201.1"
                     /locus_tag="SCNRRL3882_RS26440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_5195"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_029181651.1"
                     /sec_met_domain="melC (E-value: 1.3e-46, bitscore: 157.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVSIGGGPAGGGAPTPARPERAGRTRREVTRGLRACALAATLAA
                     PVAAAFRLRRPGTENVLGEAAFDQTYRSRRIRGTWTPSAGSGGRGRWHVTVDGRPLHLM
                     RRADGTWLSMVDHYCSYRSPLEAARAAVDELGPGRRLRDLAPEPADAGHVHTGDRHGVR
                     A"
     gene            5479..6345
                     /locus_tag="SCNRRL3882_RS26445"
                     /old_locus_tag="SCNRRL3882_5196"
     CDS             5479..6345
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="SCNRRL3882_RS26445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_5196"
                     /product="tyrosinase family protein"
                     /protein_id="WP_010046795.1"
                     /transl_table=11
                     /translation="MAYVRRDAGTLTKTEKRRFVKALLEVKRRGEYDEFVRMHIEYFSS
                     DGEHGLRAAHMAPSFLPWHRRFLLDLESALRRVDPSVTVPYWDWTRYRTTTSAPWTADL
                     LGGTGRPSDHQVMTGPFAYREGNWTIKVNVTDAVFLTRDLGRRRSPMELPTKNELQWAL
                     KDPVYDVAPWDSTVTRGFRNKLEGWGTGGGSTSWRNHNRVHGWVGGDMLGGASVNDPVF
                     WLHHAFVDLQWYRWQRRHRGARYLPAAPPGSGDGQHGRIVARHQRLPPWDVTPDELEDV
                     GRIYRYA"
     gene            complement(6426..6734)
                     /locus_tag="SCNRRL3882_RS26450"
                     /old_locus_tag="SCNRRL3882_5197"
     CDS             complement(6426..6734)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020115467.1"
                     /locus_tag="SCNRRL3882_RS26450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_5197"
                     /product="chaplin"
                     /protein_id="WP_040904180.1"
                     /transl_table=11
                     /translation="MSRIAKVGVVALGTSAVVLSGAGLAAADAGAKGAAIGSPGVLSGN
                     VVQVPVHVPINVCGNTVQFVGLLNPAVGNTCKNEGHKKDDHKKDDHKESSKKSGHRH"
     misc_feature    6997..7010
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.46"
                     /tool="antismash"
     misc_feature    6997..7011
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.71"
                     /tool="antismash"
     misc_feature    complement(6998..7011)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.11"
                     /tool="antismash"
     misc_feature    6998..7012
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.11"
                     /tool="antismash"
     misc_feature    complement(7026..7039)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.96"
                     /tool="antismash"
     misc_feature    complement(7027..7040)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.09"
                     /tool="antismash"
     gene            7120..7380
                     /locus_tag="SCNRRL3882_RS26455"
                     /old_locus_tag="SCNRRL3882_5198"
     CDS             7120..7380
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003990343.1"
                     /locus_tag="SCNRRL3882_RS26455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_5198"
                     /product="hypothetical protein"
                     /protein_id="WP_010046792.1"
                     /transl_table=11
                     /translation="MRNFQKAAVVMAMLGSVSFLGAGVGHASDGDKIKLDNDQKQACAQ
                     NDAQTGLVQLDDVNVSVGLLGWSQQDNSEKESLACSQSFGK"
     misc_feature    7702..7711
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: medium, score: 15.86"
                     /tool="antismash"
     misc_feature    7703..7716
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.03"
                     /tool="antismash"
     misc_feature    7704..7717
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.21"
                     /tool="antismash"
     misc_feature    7704..7718
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 19.95"
                     /tool="antismash"
     gene            7825..8091
                     /locus_tag="SCNRRL3882_RS26460"
                     /old_locus_tag="SCNRRL3882_5199"
     CDS             7825..8091
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010046792.1"
                     /locus_tag="SCNRRL3882_RS26460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_5199"
                     /product="hypothetical protein"
                     /protein_id="WP_010046789.1"
                     /transl_table=11
                     /translation="MRNFQKAAVVMAMLGSVSFLGAGVGHASDGDKIKIDNDQNQACAQ
                     NDAQTGLIQLDDVNVSVGAILGWSQQDNSEKESLACSQSFGKH"
     gene            8234..8512
                     /locus_tag="SCNRRL3882_RS26465"
                     /old_locus_tag="SCNRRL3882_5200"
     CDS             8234..8512
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010046783.1"
                     /locus_tag="SCNRRL3882_RS26465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_5200"
                     /product="hypothetical protein"
                     /protein_id="WP_010046787.1"
                     /transl_table=11
                     /translation="MLDTKKIAAVAAAGVLGGFALIGGGVVQAAAQGGPGRCVDDGKGH
                     VHCVQTSTYKLTTSKDGGVHLSNESTQTCPASRGQVTCVDSVVLPGR"
     misc_feature    8586..8602
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.73"
                     /tool="antismash"
     misc_feature    8599..8612
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.12"
                     /tool="antismash"
     misc_feature    8600..8613
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.06"
                     /tool="antismash"
     misc_feature    8601..8614
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.28"
                     /tool="antismash"
     misc_feature    8601..8615
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.73"
                     /tool="antismash"
     misc_feature    complement(8602..8615)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.8"
                     /tool="antismash"
     misc_feature    8602..8616
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.96"
                     /tool="antismash"
     gene            8726..8998
                     /locus_tag="SCNRRL3882_RS26470"
                     /old_locus_tag="SCNRRL3882_5201"
     CDS             8726..8998
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826206.1"
                     /locus_tag="SCNRRL3882_RS26470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_5201"
                     /product="hypothetical protein"
                     /protein_id="WP_010046785.1"
                     /transl_table=11
                     /translation="MRNYQKAAVVMAMLGSVSFLGAGVGHAADGDEKVKLENKQNQSCE
                     QNDSTKGLINIDDVNISLGILGWSEQDNSERESVTCSQNFILGGK"
     gene            9144..9428
                     /locus_tag="SCNRRL3882_RS26475"
                     /old_locus_tag="SCNRRL3882_5202"
     CDS             9144..9428
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003990342.1"
                     /locus_tag="SCNRRL3882_RS26475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_5202"
                     /product="hypothetical protein"
                     /protein_id="WP_010046783.1"
                     /transl_table=11
                     /translation="MLNSKKIAATAAAGVLGSLALIGVGAAQAAAQGGPGKCVDDGNGQ
                     VRCVQGDTYKITTAKDGSVRFANNSTQTCPTSHSQVTCVSNVIVPGKQS"
     misc_feature    complement(10341..10355)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.09"
                     /tool="antismash"
     misc_feature    complement(10400..10413)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.61"
                     /tool="antismash"
     misc_feature    10419..10433
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
ORIGIN
        1 cgggtgacgc agtacggcgg cggccacgcg ctgcgcggtc gccgccgggc cgaggacgag
       61 tgcggtgtgc gggtgccgca gcagcgcggc gcgccgccgc gcgtgcaccg ctccgcgcag
      121 cacacagccg gccgccgcgt gcagggcaag gcccgtcagc acggtacgga gcgtcaacgc
      181 gtgctccggc cggtacgccg ccaggagcgc cgcgagtgcc agccaggcca ccgcgatgcg
      241 gccgcacacg gcgggcagtt cctccagcag ccccggtacc ggcggccgcc cgtcgcgccg
      301 gcgcagcagc agcgacatga cgatcagcag ggtgacgagc agtgggtggc gctgggcatc
      361 ggtgagcgcc ggcgcgcccg ccagggcggc cacgccgtcc gcgacgagca gcgccacggg
      421 ggaggcggga cgggccggcc gccgcggtgc ggacggccgg aaaccggtgg cggtgccgcg
      481 cggcggcatg accgagactg gtgaggatcc gtggtcccgt ggcggtacgc cgggggaggg
      541 cacggtacgt tcggcggtca cgagtggatg gactccctgt actcggtggg ctcgacgcgc
      601 tgtggtgtcc ggttcgcctc ggcgccacgt ccggtgccga gcggctgacc gtgctcagcg
      661 ctgggggtgg cggcggtcca tgggccgagc aggtcgcgat agacgtccga gacggcctcg
      721 gcggtgtagc gcacgtcgtg ggtggacagc acgtgccgac ggccctggct gccgagcgac
      781 tcgcgcagca gcggatcggc cagcaactcg gtgacggccc cggccagcgc ggccgggtcc
      841 tccggcggga ccaggcaccg ggcggcgaag gagggtggca ggctctcgcg ggcgccgtcg
      901 acgtccgtga ccaccacggg ccgcccgcag gccatcgcct ccagcggcgc cagggccatg
      961 ccctcccagc gggacggcag gacgacgaga tcggaggcct ggtaccaggg ggaggcgtcg
     1021 gcgacggctc ccgtgaacac cacggattcc ggggcgcctt cgcggagccg gtcgtggtcc
     1081 ggcccgtcgc cgacgaggac caggcgggcg tcgggcacct gccgcacgac ggcttcccac
     1141 gcccgcagca ggacgtcctg ccccttctgc cggcacagcc gccccacgca cacggcgagc
     1201 ggtgccgccg ggcggatgcc ggcgagcggg gcgagcccgg cccgtacggc gcccgcgggg
     1261 gccgggcgga agcgctccgg gtcgatgccg ttggggacga cggtccaccg gccggtgatc
     1321 ctggcgtcca tgccggtggc gcgttccgcc tcgctcacgc acaccacgcg tgaggcccaa
     1381 cgtgcccccc agcgttccca taggagcgcc agggccgcgg tgcccccgct gaccgcttcg
     1441 aacgaccagg cgtgcggctg gaagaccgtc ggaatccgcc cgcgtaccgc gagccgtccg
     1501 gcgagcccgg ccttcgcgct gtgcgcgtgc accagatcgg ggcgcacctc gtcgatcacc
     1561 cgcgccaggt gccgcacctc ccggacgagc gacgtacccg gcgaccgtgt cgcgtgccag
     1621 tgccgtacgc gcgcgccgag cgtccgcagc ttcgtggtga gcgcgctgtc ggggcaggcc
     1681 acggtgacgt ccaggccgtc cgacagttgg gcgcggacca gatccgtcac gacgcgcgcg
     1741 acgccgccgt ccaccggttg ggtgaggtgc aggactcttg ggaggggggc ggtgtgcatg
     1801 tgggcggatt cctcgctcgc gaaggtgttc ggggcgggcg gcgacggagt caccgccgtg
     1861 cgtcgacggc ggcgaagagc acgccggccc acgccgcgtc ctgctgggag tggagccgga
     1921 aggtcgcttg gtcaccggcc tgctgcaggc cacttccgag atcgaacacg tcggagtcgt
     1981 agccgaggtt gttggcgtgc gcgggcaccc gtgccgcatc cgccccgggc tcgctgatgg
     2041 tggaattgag gacgtcgtcc cgggggttgg cggcgttggt gagggcgaac tcggactggc
     2101 cggtggtcac ggtgagcgag tcgccggtgg tgccgcggtc gccgttgtac gtcaccaggc
     2161 cggcacggcc ccacgcgccc gcggcgaagc ccagatcggt catccggatc tcctgtgccg
     2221 caccgggcct cagcggggtg aagccgtccc agagcgcgag gtgacgcagt ggctccgacg
     2281 ggttctcgta ggccaccacg agcgtccagc caccccacgc cccggccgcc gatctgccca
     2341 tggcgatgtt gatctgcgcc acggtgtaca ggccgggccc gccctcccgc accagccgtg
     2401 tcacgtcggc cgaggcctgg aaggcgtcca tgccgtccgc cacccggtgt ccgaccgtcg
     2461 tgtcggcgag caactccttg tactctccgc cgggttcggc gatcagcacg cgctcgttgt
     2521 cctgcggcgg cttctgttcg cccacgcgca ggttgccgcc ccagtagagg cgcgcgtacg
     2581 tcacccggga ggcgttgggg aggcggacct ccgcgcggga ggagttgtag gtgttggggt
     2641 cgcggtcgac atcgacgtag aacatgtcga agtcgccgtt cacaccggac ccgcctgcct
     2701 gggcgtccgg gcacgaaggc gcctgggccg tgcggcaggt gatcgacgcg ttggccgcgc
     2761 gtacgatgcc gccgtgctgg agcgtgcggt accgctcggc gaagccgagg ctctcggcct
     2821 ccggcgcggg cgcggcggcc ggagcggcgg ccggggaact gcttgctgcc cacacggccg
     2881 caagggccag gacgcccacg gtcgcgcggc gaaacacggg gcccagagaa tggcgcatga
     2941 ccggtcgttg ccctttcgga acaggtggcc gtggcagcag tgccacgaga gcgatgtgtc
     3001 cgcgctgaga agtgcgtcgg gcggttcgca actctagtca tcaatcggac atagtcgaag
     3061 aaattcggtg aagtctgaca agtgtgtcgg aatcgtgaag cagcaagtcc ttgctggttc
     3121 cggccgtcgc ctccggcctc ggtcagcgcc tccattcggg ggaggccggt gccttgctcc
     3181 ggcaggggcc gagaaggggg cgtcctgaca taccgtctga tcagggcggg cacggcgaat
     3241 gggggaattg ttaaatcccg aaaagcctat gagcggttgc gggaagtcgc gggcgctttc
     3301 acggtgggct caagatccaa cgccgtcgaa aggaatctcc atgtgtgctc tgcccgcacg
     3361 gcgcatcgcg tccagcgccc tgtgcgccgc ccttctggtc gggatcaccg gccccgtagc
     3421 catggcggcc gactcggctc gcgggcacgc tcatgtggcg cccgacgccc ggcttcccgg
     3481 cgcggatgcc cgtctcgcgc agatcgacag actcaactgg ggtgagctca ccccggtcgc
     3541 cgacctgctg cacgccgtgc tccggagcga cggccgactg cccgcgtccg aggccaggaa
     3601 gctgggagcc gaagcgaagg ccgccctgac cgaggcggcc gccaagggcg agcgaacctc
     3661 gatgacgtcg acggtcgtgc tgttgccggc tcccgccctg cccgccctgg tgcccggggc
     3721 gcccgaccgg cgcgccgccg accccgtgac cgacctgctg gacctcgtgc tgggagctgt
     3781 cgacagtctc ctgcaggcca tcacctccgg tgtcggcggc atccttccct ccgtcgacga
     3841 cctgctgaac ggggtggacg acctcctcgc cggactgatc ggaggcaacc tgcttcagga
     3901 cgattccgtt tcgtcgccgt cgtccagcac gtcgtcgaca tcggtgccga cgacgatgcc
     3961 cgcggccgtc acgtacccgg ccgtcccgct gctgacgcgg gtggtgccgc cggcctcctg
     4021 aaccgcgtcc agtcgtggtg ccgacctcag ggagcggcgc cacgactgtc gttcttcccg
     4081 cccgacttcg cgtattcctc cgattcattt tccgttccgt ggcccggtct gttaatagat
     4141 ctcgtatgag gtatcggcaa tcttgggcat ctccggtttc cgcccaggcc agggctcgtt
     4201 aggcacggcg acagaattcc ctccggcgac gcgcgttgcc gaagaaagga acacgatgaa
     4261 gtctctgaag gctgccgccg tctttgccgg gtccttgatc gctgccggtt ccgccacacc
     4321 cgcgttcgcc caggacgcca tggtcgcccc gagcgcgccg gccgctcaga gcgttccgga
     4381 cgtgcaggct gccccggtcg cccagagcgc tcccgttgct caggccgctc cggttcagag
     4441 cgctccggct gttcagagtg ctccgatggc tcagcccgcc ccggctccgg tcgctcagcc
     4501 tgccccggtt gcgcagccgg ctccggttgc tcagcccgcc ccggctcctc agccggctcc
     4561 ggtcgctcag cccgccccgg ctcctcagcc ggctccggtt gctcagcccg cccccgctcc
     4621 gcagccggct ccggccgctg agaacactcg ggacctggcg tccaccgacc tcaccggcgg
     4681 cgtgagcacc agggttcccc tagatgtgag cgctcagggc aaggaatcgc tggtccagac
     4741 ggcgaagaac gccgcgtccg cggtcaacga caccaagccg gtgcacggca acctgtggtt
     4801 ctagagctgg gaccacgcgt acggagggtc ggtcctcgga cagttcgagg gccggccctc
     4861 cgttgtgtgt cctgccggtg acgcgacccg cccccctacc ggcagctacg ccggcgatca
     4921 cttgtctggc cgcgcggcgg tgaccgcgcg ggacgtccgg cggttacggc tacgacaccg
     4981 gaaccggacg aggagtggac atggtcgtca gcatcggcgg aggaccggcg ggaggcgggg
     5041 caccgacccc ggcgcgaccc gaacgggcgg gcaggacacg gcgggaggtg acgcgcggtc
     5101 tgcgtgcctg cgccctcgcc gccaccctgg cggccccggt ggccgccgcc ttccggctcc
     5161 ggcgccccgg aaccgaaaac gtcctgggcg aagccgcttt cgaccagacc taccgcagcc
     5221 gccgcatccg agggacgtgg accccgtcgg ccgggtcggg cgggcggggt cggtggcacg
     5281 tcactgtgga cggccgaccg ctgcatctga tgcgccgggc cgacggcacc tggctgagca
     5341 tggtcgacca ctactgctcg taccggtcac cactcgaagc cgcccgcgca gccgtcgacg
     5401 aactcggccc cggccgccga ctgcgcgacc tggccccgga accagcagac gcggggcatg
     5461 tgcacacggg ggaccggcat ggcgtacgtg cgtagagacg ccggcacgct caccaagacc
     5521 gagaagcggc ggttcgtcaa ggcgctgctg gaggtcaaac ggcggggcga gtacgacgag
     5581 ttcgtgcgga tgcacatcga gtacttctcc tccgacggcg aacacggact gcgcgccgcg
     5641 cacatggcac cctccttcct gccctggcac cgccggttcc tgctcgacct ggagagcgcg
     5701 ctgcgccggg tggacccctc ggtgaccgtg ccgtactggg actggacgcg ctaccgcacg
     5761 accacctccg caccctggac ggcggacctc ctcggcggca ccggacgccc ttccgaccac
     5821 caggtgatga cgggtccgtt cgcctaccgg gaaggcaact ggaccatcaa ggtgaatgtg
     5881 accgacgcgg tgttcctcac ccgggacctc ggccgccgcc gctcccccat ggagctgccc
     5941 acgaagaacg agctgcagtg ggcgctgaag gacccggtct acgacgtggc gccctgggac
     6001 tcgacggtca cgcggggatt ccgcaacaaa ctggagggct ggggcaccgg tgggggcagc
     6061 acgtcctggc gcaaccacaa ccgggtgcac ggctgggtcg gcggggacat gctcggcggc
     6121 gcttccgtca acgaccccgt gttctggctg caccacgcct tcgtcgacct gcagtggtac
     6181 cgctggcagc gcagacaccg cggggcccgt taccttcccg cggcaccgcc gggctccggc
     6241 gacgggcagc acggccggat cgtggcacgg caccagcgcc tgcctccgtg ggacgtgacg
     6301 ccggacgaac tggaggacgt cggccggatc taccggtacg cgtgaggtga cgaggaccct
     6361 cacggcgaca tggggagagc cccggcgttc ggggaggccg gggctctcgg ggcagacagc
     6421 agacgtcagt ggcggtggcc gctcttcttg ctgctctcct tgtggtcgtc cttcttgtgg
     6481 tcgtccttct tgtgaccctc gttcttgcag gtgttaccga cggccgggtt gagcaggccg
     6541 acaaactgga cggtgttgcc gcacacgttg atcggcacgt ggaccggcac ctgaacgacg
     6601 ttgccggaca ggacaccggg cgagccgatg gcagcgccct tggcgccggc gtccgccgcg
     6661 gcgagaccgg cgccactgag caccacagcg ctcgtgccga gagcgacaac gccaaccttc
     6721 gcgatgcgag acatcacgtt ctccttagtg aatcggtgag tgcggcagca gtacgcgcga
     6781 ccgcactgac cgtttcaacg cgagggcccc ggaccggtaa cgaggttttt ctccggatca
     6841 ccctttttga acctgtgctc cgccgtacgg gcgttgtttc cccacgaccc gtgccgtgcc
     6901 ggggtcatgc cgcggcgaag acgggctccc tgtctcgtcc ggcacgcgac gcgccacggc
     6961 ggctgctcgg cgtctgcccg gcagctccgc acggagattt tttgggaaag ttaagcgatt
     7021 gggcgttttg cgtataaaaa ccatcgatct tcacctacag tctgtgactg ccagtgacca
     7081 gtccatcacg gattgtgtcg actcgagaac ggagaggtaa tgcgcaactt ccagaaggcc
     7141 gcggtcgtca tggccatgct cggcagcgtc agcttcctgg gtgccggtgt cggtcacgct
     7201 tcggacggcg acaagatcaa gctcgacaac gaccagaagc aggcgtgtgc ccagaacgac
     7261 gcgcagaccg ggctcgtcca gctcgacgac gtcaacgtct cggtcggcct cctcggctgg
     7321 tctcagcagg acaacagcga gaaggagtcc ctggcctgct cgcagagctt cggcaagtga
     7381 cgatcggctg atctgaaggc aggccccggg gttcgaccat gtgtccgaac cccggggcct
     7441 gctgcttgtg gtgcggtcga cgcgggcgcg ttcgcgcgat cggctcctga aggtgcgcgc
     7501 ggccgccggc cgcgacccgg cgaccacctc gacacgcacc gccgagacgg caggcgctct
     7561 tcgtgttcgg tggcgagggc ggcctcccgc ctgtggagaa ggatccgccc gctgtgaaca
     7621 gcggctgtcc gaccggtagt tggccgacgg tggcggtcct ggtcgctggt cgccggacac
     7681 ctgcacagca tcaccacaat gtgttttttc aggaaagtta cgcgattggg cgttttgcct
     7741 atcaaaagcc ccgaatgtca cctagagtgc gactgtcagt gaccagtcca tcacggattg
     7801 tgtcgactcg agaacggaga ggtaatgcgc aacttccaga aggccgcggt cgtcatggcc
     7861 atgctcggca gcgtcagctt cctgggtgcc ggtgtcggtc acgcttcgga cggcgacaag
     7921 atcaagattg acaacgacca gaaccaggcg tgcgcccaga acgacgcgca gaccgggctc
     7981 atccagctcg acgacgtcaa cgtttcggtc ggtgccatcc tcggctggtc ccagcaggac
     8041 aacagcgaga aggagtccct ggcctgctcg cagagcttcg gcaagcactg atccagtgat
     8101 ctgaaggcag gccccggggt tcgaccacgt gtccgaaccc cggggcctgc tgtctggttt
     8161 ccagccggtc catatgcccg gaaaaacgac tccgccgttc agccgcgggg ggtcgaccgc
     8221 aaaggagaaa cagatgctcg acacgaagaa gatcgcagcg gtggcggcag ccggagtcct
     8281 ggggggcttc gctctgatcg gtggcggagt cgtccaggcc gctgcccagg gcggccccgg
     8341 caggtgcgtc gacgacggca agggtcacgt tcactgtgtg cagacgagta cgtacaagct
     8401 caccacgagc aaggacggcg gagtccacct cagtaacgag tcgacgcaga cctgtccggc
     8461 ctcgcgcggt caggtcacgt gtgtcgacag cgtcgtcctc cccgggcggt agcccccggg
     8521 ggttccctag cggtagttgg tcggaagtgg ctagttgagt taggccatcc gatacataca
     8581 aaaccgcgtt acaacgcaac ttttttcaag aaacttgcgt gagtgggcgt ttcgcccgtt
     8641 agaagccgcg tgagggtcac ctacagttgg cgactgtcgg tgaccaaccc atcacgggtt
     8701 gtgtccactc aagaacggag cagtaatgcg caactaccag aaggccgcgg tcgtcatggc
     8761 catgctcggc agcgtcagct tcctgggggc cggtgtcggc cacgctgcgg acggggacga
     8821 gaaggtcaag ctggagaaca agcagaacca gtcgtgtgag cagaacgact cgaccaaggg
     8881 cctcatcaac atcgatgacg tcaacatctc gctcggcatc ctcggctggt cggagcagga
     8941 caacagcgag cgcgagtccg tgacctgctc ccagaacttc atcctcggtg gcaagtgatc
     9001 actgatcagg tgatctaggc aggccccggg gctcgaacca ggtggtccga gccccggggc
     9061 ctgtcgtctg gcttctttgt cggcccaagc gtccggaacg accccgccgt ccagtcacgg
     9121 ggtcgaccgt aaaggaaaag tagatgctca actcgaagaa gatcgcagct acagcggcag
     9181 ccggagtcct ggggagcctt gccctgatcg gggtcggtgc cgcccaggcc gccgcccagg
     9241 gcggccccgg caagtgcgtg gacgacggca acgggcaggt ccgctgcgtg caaggggaca
     9301 cgtacaagat cacgaccgcc aaggacggca gcgtccgctt cgccaacaac tcgacgcaga
     9361 cgtgcccgac ctcgcacagc caggtcacct gcgtcagcaa cgtcatcgtt cctggcaagc
     9421 agtcctgaag ttcgacgcgg cagccgaggc gcccttgttc acgtcggcgc ccgtggcgtg
     9481 aatccaccgc tcacagcggt ggccggccgg gacccgtcac cgggtcccgg ccgtttccgt
     9541 gtcctcaccc ggacggtgcg gaaacgtcct cacccggacg gtgcggaaac ccggcgcggc
     9601 cccggacggg cgtcacggca gccgtcgtac gggcgcgccc ccgaggaacg cgcggatgtt
     9661 ctcgaccgcc tgcccgtagt acgtcgcgta gttggcccgg gagacgtagc cgaggtgcgg
     9721 cgtggcgagg aggcgggggg cggtgcgcat cgggtggtcg gcgggcaggg gctcgatgtc
     9781 gaagacgtcg atgccggcgc cggcgatccg gccctcgtgc agggcggcga ggagggcttc
     9841 ctggtcgacg atggcggcgc gcgaggtgtt gatcaggtac gcggtcggct tgaggatggc
     9901 gagttcggcg ggtccgagca gtccgcgggt gcggtcgctc agcgcgaggt ggatcgagac
     9961 gaagtcgctg tcggcgagca ggtcctcccg ggagggggcg agttcgacgc cgatctcgtc
    10021 ggtgcgctcc tctgtgaggt tctgactcca cgcgctgacg tgcatgccga aggcgagacc
    10081 gacccgggcc acgcggctgc cgatcttgcc gaggccgaga agtccgaggc ggcgtccgtg
    10141 caggtcggcg ccgacggtgg attgccaagg gccgccggag cgcagggcgt tgttctcctc
    10201 gacgagcccg cgggcgagac cgagcagcag ggcccacgtc agttccacgg gcggggtgga
    10261 ggagctggtg gtgccgcaca cggtgacgcc gtgcttctcg gcggccgcgt agtcgatgac
    10321 cgagttgcgc atgccggagg cgacgatgag tttcagccgg ggcaggcggg cgatcagcga
    10381 cccggggaag ggcacgcgct cccgcagcgt caccacgagg tcgaagtcgg ccagggccgc
    10441 ggccagggcg tcctcgccgt ccaggtgggt gtcgaaggag acgacctcca cctgg
//
