LOCUS       NZ_LT963352            11053 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Streptomyces chartreusis NRRL 3882 isolate NRRL3882 chromosome I.
ACCESSION   NZ_LT963352
VERSION     NZ_LT963352.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces chartreusis NRRL 3882
  ORGANISM  Streptomyces chartreusis NRRL 3882
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wibberg,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2017) CEBITEC, Genome Research of Industrial
            Microorganisms (GRIM), Universitaetsstrasse 27, 33615, NRW, Germany
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:03:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,073
            CDSs (total)                      :: 7,984
            Genes (coding)                    :: 7,779
            CDSs (with protein)               :: 7,779
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 91 of 205
            Pseudo Genes (incomplete)         :: 161 of 205
            Pseudo Genes (internal stop)      :: 14 of 205
            Pseudo Genes (multiple problems)  :: 56 of 205
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:02:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4497001
            Orig. end                         :: 4508054
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT963352.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11053
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="17"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11053
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11053
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6053](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6053
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(1138..1467)
                     /locus_tag="SCNRRL3882_RS20145"
                     /old_locus_tag="SCNRRL3882_3978"
     CDS             complement(1138..1467)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1163: rhodanese domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019055193.1"
                     /locus_tag="SCNRRL3882_RS20145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_3978"
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="WP_010032550.1"
                     /transl_table=11
                     /translation="MPTVEVADLKDGDFLLDVREDDEWKAGHAEGALHIPISDFVARYG
                     ELTEAAPQDGRIHVICRSGGRSAQVTMYLAQQGIDAVNVDGGMQVWEAAGRPVVTDDGQ
                     PGFVL"
     gene            complement(1541..2455)
                     /locus_tag="SCNRRL3882_RS20150"
                     /old_locus_tag="SCNRRL3882_3979"
     CDS             complement(1541..2455)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016437092.1"
                     /locus_tag="SCNRRL3882_RS20150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_3979"
                     /product="hypothetical protein"
                     /protein_id="WP_231911149.1"
                     /transl_table=11
                     /translation="MTTPEAEQGGGGAGEERVTPEGDAARAEDGEVRPEDGAAQSEGEA
                     ARPEARLEKAVRAAEQALIEYEIAVETFRVEVENFSRLHEQKLGPVYARLEELDARILE
                     ARAARSGDAEDRRRAEEARARLAPIPGVEELLHGWMDDSGLFPEAVAMLTDQAVRPPQR
                     VRPSEEARKLYRELARKAHPDLAQEEQERARREEFITRVNAAYARGDEAELRELAEEWA
                     KGPELKRGPSRAEELYARLEWLAQRKELLALLAKELEESAIGHMLQLAPDDPDGLLDEI
                     GQQLLTQVTERERELAALLADTP"
     misc_feature    2525..2540
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 18.33"
                     /tool="antismash"
     gene            complement(2526..4046)
                     /locus_tag="SCNRRL3882_RS20155"
                     /old_locus_tag="SCNRRL3882_3980"
     CDS             complement(2526..4046)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020119458.1"
                     /locus_tag="SCNRRL3882_RS20155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_3980"
                     /product="DUF2252 domain-containing protein"
                     /protein_id="WP_231911150.1"
                     /transl_table=11
                     /translation="MSTGSDAFRPSAVRSARVDVRARAGEAVGVTRDGAGAEGGAAHGA
                     TRRLPRVRGFAEWPVEGSPKDEGKALRKRIPRSAHATLDPDESRPDAVSAVEESGRGRL
                     PELTPIRVGRMAATPFAFLRGAAGLMAHDLARTPMTRIRAQICGDAHAANFGLYGDARG
                     GLVIDLNDFDETVLGPWEWDVKRLAASLVLAGREAGADEDRCRKAACDAVGAYRRTMRL
                     LARLPVLDAWNAIADEELVSHTDAHDLLGTLERVSEKARHNTSGRFAAKSTEATEDGGR
                     RFVDAPPVLRRVPDEEAAAVAASLEEYVGTLPEDRLPLLARHAVHDVAFRVVGTGSVGT
                     RSYVVLLLDHRGEPLVLQVKEARASALVPHLVTAGFDAPAAGHEGRRVVLGQKRMQVVS
                     DILLGWTTVDGRPFQVRQFRNRKGSVDPAALAADQIDDYGRMTGALLARAHAHSADPRL
                     IAGYCGKSEELDEAMAAFAVAYADRTEADHADLVAAVRAGKVAAETGV"
     gene            complement(4138..4842)
                     /locus_tag="SCNRRL3882_RS20160"
                     /old_locus_tag="SCNRRL3882_3981"
     CDS             complement(4138..4842)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004929054.1"
                     /locus_tag="SCNRRL3882_RS20160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_3981"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_010032559.1"
                     /transl_table=11
                     /translation="MARQERAIRTRRTILEAAAAVFDERGYTSATIGEILDRAGVTKGA
                     LYFHFGSKEELAVGVFEEQLGITLPPQSSKLQELVDSGLVLARRLRSEPLVRASVGLAL
                     DQGAMDLDRTPAFRMWIDQNVQMLAEAKAQGELLPHVDVAETAELLVGSFSGVQLLSQL
                     RCQRQDLERRIIVMFEHFLPSIAVPAVLARLDISAERAERVLAQGQVEVPVQGRVQVQE
                     GAVPAAQVSSGA"
     misc_feature    complement(4853..4866)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.21"
                     /tool="antismash"
     misc_feature    complement(4923..4937)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.42"
                     /tool="antismash"
     misc_feature    4924..4937
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.64"
                     /tool="antismash"
     misc_feature    4925..4938
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.63"
                     /tool="antismash"
     misc_feature    4926..4939
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.06"
                     /tool="antismash"
     misc_feature    4927..4940
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.43"
                     /tool="antismash"
     misc_feature    complement(4931..4940)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 12.94"
                     /tool="antismash"
     gene            5001..6053
                     /locus_tag="SCNRRL3882_RS20165"
                     /old_locus_tag="SCNRRL3882_3982"
     CDS             5001..6053
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004929057.1"
                     /locus_tag="SCNRRL3882_RS20165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_3982"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_010032560.1"
                     /sec_met_domain="AfsA (E-value: 1.3e-21, bitscore: 75.3,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQSEPLSAPTDDQTGQEGRPANRTAAAVENAPQAAATDSAETTE
                     APSVTSVTKELAHRTTEADVFPVRWRRISETRFRFTAHWPADHAFFGPVDDRHQDPMVV
                     GETLRQASMVLAHAEFGAPADTHFVMWDLTVHTDPSALLLSDAAQPVEIDVVCSEIRRR
                     GRGLHSMRTTMEFRRAGRFVARGTGSTGCTSPLAYRRLRAAQLAEVGTPVPLLPGISPA
                     LAGRSRAEDVVLAPADRPGVWRLRVDTGHPVLFPRPNDHVPGMVLFEAARQAATAASGR
                     HPFLPRSMAATFSRYAELHSPCWIETEVLDEGPGDPGETTVRVSGRQDGESVFSATLTC
                     PLPARVRSLS"
     misc_feature    5019..5021
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6050..6985
                     /locus_tag="SCNRRL3882_RS20170"
                     /old_locus_tag="SCNRRL3882_3983"
     CDS             6050..6985
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003991560.1"
                     /locus_tag="SCNRRL3882_RS20170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_3983"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_010032561.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 5.4e-08, bitscore:
                     31.1, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVDLLITGTSGFVGSRVAAAAERQPGVRVRHLSRRTPPEAGPGA
                     FVHGDLGDPPSLRGSCAGTDVLVHCATRIGGDAGTVEAMNDLGTRALVEEAVRAGVSRI
                     VYVSTAAVHGRGPFRGVRPGEVPIAPASATSRTRAAAERHVLDAGGLVLRPHLVYGEGD
                     RWAVPGLVWLLGELSTTALTGCAALQSMIDVDSLGRAVVAAALSPAHDGGVHHVNHPEP
                     VSVPDLLGAVGTLLEEPGGGAAVDVATALDRAAGKPLALHHLGMLTVDHWFVDDAFWKD
                     LDCSPGEGFAAEFRRAAPWYRSFLRGRRAS"
     gene            complement(6930..8270)
                     /locus_tag="SCNRRL3882_RS20175"
                     /old_locus_tag="SCNRRL3882_3984"
     CDS             complement(6930..8270)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003991559.1"
                     /locus_tag="SCNRRL3882_RS20175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_3984"
                     /product="cytochrome P450"
                     /protein_id="WP_010032564.1"
                     /sec_met_domain="p450 (E-value: 1.1e-22, bitscore: 79.3,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQHACPFLAIDPSGQDLYGEIARIRAQGPAVEVELPGGVRAWWI
                     NGLELNKRLLAGPETSKDAFQHWPAWINGDISRTWPLAIWVSVRNMVTAYGTDHSRLRR
                     PMAAAFTKRRVDALLPRVQEIVDRALDDLERIPEGEVVDLRAGFAAPIPHEVVCELFGV
                     PPGEDGPRSALYRIINRFFDTAITLEDAQANGVELYGTLTAFLQYKREHPADDLTTALM
                     AAHDEGSLSEQELMDNLILLLTAGFETTVNLIDNTVHSLLAHPDQLELVRTGQVTWEDA
                     IEESLRFEAPGAMSGLRYAVEDIEIEPGLTIPKGDPVVVSFAGAGRDPERHGPDAERFD
                     VTRSTSRDHVSFGHGVHHCLGRPLAMAEATVALTSLFDRFPRLALADPSRPPQRLRSII
                     STGHQELPVLLHGRGAGSQPGPRPEEAEAIRKLSGLGGTSGTRVPRV"
     misc_feature    8318..8331
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.52"
                     /tool="antismash"
     misc_feature    8329..8349
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.62"
                     /tool="antismash"
     misc_feature    8385..8399
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     gene            complement(8415..9137)
                     /locus_tag="SCNRRL3882_RS20180"
                     /old_locus_tag="SCNRRL3882_3985"
     CDS             complement(8415..9137)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003991558.1"
                     /locus_tag="SCNRRL3882_RS20180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_3985"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_010032569.1"
                     /transl_table=11
                     /translation="MTKQDRAVRTRLALIRSAAEQFEARGYIRASLAEISAGAGVSSGA
                     LHFHFANKAAVAEAVERAAARALRRVAARGRGGDGCALQRLVDVTHHVARLLCADIVVR
                     AGLRLNAEAVAGRVRDLRREWRECVHELLAEAERRGELAAGMPPHRSSAVVLAATTGIE
                     VLAREDPGWLARPSLTDLWHLLLPCVAAPHLAGALDPAGTGPDHADPHADGEADVGREA
                     DTADGALEEPGRAGLALR"
     misc_feature    9188..9197
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.41"
                     /tool="antismash"
     gene            complement(9291..9950)
                     /locus_tag="SCNRRL3882_RS20185"
                     /old_locus_tag="SCNRRL3882_3986"
     CDS             complement(9291..9950)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010985061.1"
                     /locus_tag="SCNRRL3882_RS20185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SCNRRL3882_3986"
                     /product="response regulator transcription factor"
                     /protein_id="WP_029180671.1"
                     /transl_table=11
                     /translation="MRVERPLRVVLAEDSVLLRDGLVGLLGRCGHEVVAAVGDAGALIA
                     AVGEHAPDVVVTDVRMPPGFQDEGLHAAVRLRQERPTLPVLVLSQYVQRRYAAELLDSG
                     DGTGVGYLLKDRVGQVEEFVEALGEVADGGTVVDPEVVRQLLRRRRDPLERLTPREREV
                     LALIAEGRSNGAIARELVVSEAAVGKHISGILTKLDLPPADETHRRVLAVLAYLRA"
     misc_feature    10091..10104
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 17.99"
                     /tool="antismash"
     misc_feature    10395..10409
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     misc_feature    complement(10449..10469)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.92"
                     /tool="antismash"
     misc_feature    10780..10794
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 22.18"
                     /tool="antismash"
ORIGIN
        1 gcggccgagg cggcgaaggg cgtgttcacc gcggtcgcgc cggacgccgt gcccagcccg
       61 gccctgactg cgggcgcggt cgccgacacc ttcgaccgcg ccctgtgggc caagctcgcc
      121 gacgccgacc tgctgagcct gctgctggac gcgcactacg gcggggcagg cctggacgcg
      181 atcgcgctgt gcctggtgct gcgggagtcg gggcgggtgc tggccagggt gccgctgctg
      241 gagaacagcg cggcagccgc cgtcgtccag gcctacggca gcgaggaggc gaaggccacc
      301 ctggcggccc gggcgggccg gggcgagctc gtgctgaccg tcgccgcaca cggccgcacc
      361 ggccacgacc cggccgagct cgccgtcacc gcacggcggg acggcgaggc gtgggtgctg
      421 gacggagtgc agacggcggt gccgtgggcg tacgacgcgg acgtcacggt cgtccccgcg
      481 cacacggaga cgggccggac cgtgctggcg ttggtgccgc gtggccacga gggggccgtc
      541 ctcaccgagc agttctccac gagcggtgag cggctcggcg agctgcggct ggagtcggcg
      601 cggctgaccg cccgggacgt gatcgacgcc gacggggcgt gggagtggct gcgggacctg
      661 ctggccaccg ggacgtgtgc gctggcgctc gggctcgggg agcgcgtgct gcggatgacc
      721 ggcgcgtaca cgagcgagcg ggagcagttc ggcttcccga tcgcgacctt ccaggccgtc
      781 gccgtgcagg ccgccgaccg ctacatcgac ctgcgcgcga tggaggccac gctgtggcag
      841 gccgcgtggc ggatcgcctc gggggccccg ggcgcgctgc ccgccaccgg tgacgtcgcc
      901 gtcgccaaga tctgggcggc ggagggcgta cgccgggtcg tgcagaccgc gcagcacctg
      961 cacggcgggt tcggcgccga cgtcgactac cccctgcacc ggtaccacgc ctgggccaag
     1021 cacctggagc tgtcactcgg cccggcggcg gcgcacgagg agaccttggg cgacctgctg
     1081 gcggcccatc ccctggcctg aacccaccgg cggctgcggc ggagggcgct acagccgcta
     1141 caggacgaac ccgggctgcc cgtcgtccgt cacgacggga cggcccgccg cctcccacac
     1201 ctgcatgccg ccgtcgacgt tcacggcgtc gatgccctgc tgggccaggt acatggtgac
     1261 ctgggcggaa cgcccgccgg accggcagat cacatggatc cggccgtcct gcggggcggc
     1321 ctcggtcagc tcgccgtacc gggcgacgaa gtcgctgatg gggatgtgca gcgccccttc
     1381 ggcatgaccg gccttccact cgtcgtcctc ccggacgtcc agcaggaagt cgccgtcctt
     1441 gaggtccgcg acctcgaccg tgggcacacc agctccgaaa ctcatgcgtc cgacgctacc
     1501 cgaaccggcc cgtgccccgc cctgacccgg acgacaggcc ctacggggtg tccgcgagca
     1561 gcgcggccag ctcccgctcc cgctcggtga cctgggtgag cagctgctgc ccgatctcgt
     1621 ccaggaggcc gtccgggtcg tccggggcca gctggagcat gtggcctatg gcgctctcct
     1681 ccagctcctt ggcgagcagg gcgagcagct ccttgcgctg ggcgagccat tcgaggcggg
     1741 cgtagagctc ctcggcgcgg ctcgggcccc gcttcagctc tggccccttg gcccactcct
     1801 cggcgagctc gcgcagctcc gcctcgtcgc cccgggcgta ggcggcgttc acccgggtga
     1861 tgaactcctc gcgccgcgcg cgttcctgct cctcctgcgc caggtccggg tgggccttgc
     1921 gggccagctc gcggtagagc ttgcgggcct cctcgctggg gcggacccgc tgcggtggcc
     1981 ggaccgcctg gtccgtgagc atcgccacgg cctccgggaa caggccgctg tcgtccatcc
     2041 agccgtgcag cagctcctcg acgccgggga tcggcgcgag ccgggccctt gcctcctccg
     2101 cgcggcgccg gtcctccgcg tcgccgctgc gggcggccct ggcctccagg atccgggcgt
     2161 ccagctcctc cagccgggcg tacaccggac cgagcttctg ttcgtgcagc cgggagaagt
     2221 tctcgacctc gacgcggaag gtctccaccg cgatctcgta ctcgatcagc gcctgctcgg
     2281 cggcccgcac ggccttctcc agccgcgcct cgggccgcgc cgcctcgcct tcgctctgcg
     2341 ccgcaccgtc ctcggggcga acctcgccgt cctcggcacg cgccgcgtca ccttcgggcg
     2401 tcacccgttc ctcaccggcg ccgccaccac cctgctcagc ttccggggtc gtcacccgac
     2461 cagcgtaggc cacggcccgg ccactcccgc gctccggaca gccgcgcgcc cgcagagccc
     2521 aggagtcaca cccccgtctc cgccgcgacc ttccccgccc gcaccgccgc caccaggtcc
     2581 gcgtggtccg cctccgtacg gtccgcatag gcgacggcga acgcggccat cgcctcgtcc
     2641 agctcctcgc tcttgccgca gtagccggcg atgaggcggg ggtcggcgct gtgggcgtgg
     2701 gcgcgggcca ggagggcgcc ggtcatccgg ccgtagtcgt ctatctggtc ggctgccagg
     2761 gcggccggat cgacgctgcc cttgcggttg cggaactggc ggacctggaa ggggcgcccg
     2821 tcgaccgtcg tccagcccag caggatgtcg ctcacgacct gcatccgctt ctgccccagg
     2881 acgacccgcc ggccctcgtg gccggccgcc ggcgcgtcga aaccggccgt caccaggtgc
     2941 ggcacgagcg ccgacgcccg cgcctccttc acctggagca ccagcggctc gccccggtgg
     3001 tccaggagca gcacgacgta ggaccgggtg ccgacgctgc cggtgccgac gacgcggaac
     3061 gccacgtcgt gcaccgcgtg ccgggcgagg agcggcagcc ggtcctccgg cagcgtgccg
     3121 acgtactcct ccagggacgc cgcaaccgcc gcggcctcct cgtccgggac gcgccgcagc
     3181 accggcgggg cgtcgacgaa gcggcgcccg ccgtcctcgg tggcctccgt cgacttggcc
     3241 gcgaaacggc cgctggtgtt gtggcgggcc ttctcggaga cccgctccag cgtgccgagc
     3301 aggtcatggg cgtccgtgtg ggagacgagc tcctcgtccg cgatggcgtt ccacgcgtcc
     3361 agcaccggga gcctcgccag cagccgcatg gtgcggcggt aggcacccac cgcgtcacag
     3421 gccgccttgc ggcacctgtc ctcgtccgct ccggcctccc ggcccgcgag caccagggac
     3481 gccgcgaggc gcttcacgtc ccactcccag gggccgagca ccgtctcgtc gaagtcgttc
     3541 agatcgatga ccaggccgcc gcgggcgtcg ccgtacaggc cgaagttggc cgcgtgggcg
     3601 tcgccgcaga tctgggcccg gatccgggtc atgggggtgc gcgccaggtc gtgggccatg
     3661 aggcccgcgg cgccgcgcag gaacgcgaac ggggtggcgg ccatgcggcc gacccggatc
     3721 ggcgtcagct cggggagccg gccgcgcccc gactcctcga ccgcgctcac cgcatcgggc
     3781 cgggactcgt ccggatcgag cgtggcatgc gcgctgcgcg ggatccgctt gcgcagggcc
     3841 ttgccctcgt ccttgggcga gccctcgacg ggccactcgg cgaaaccgcg cacccgcggc
     3901 agccgacggg tcgccccgtg cgccgccccg ccctcggccc cagcaccgtc cctggtcaca
     3961 ccgaccgcct cccccgcacg ggcacgaaca tcaactcgtg ccgaccgtac agccgacggc
     4021 cggaacgcgt cagaccctgt ggacatctcc acagctgtgg acaacccatg cggagcagaa
     4081 aggaacaggc aaggacggac agcgacactc acggggccgt cgcactcggg ccggctccta
     4141 cgcccccgac gagacctgcg ccgccggcac ggccccctcc tgcacctgca cccgcccctg
     4201 cacgggcacc tccacctgcc cctgcgccag cacccgctcg gcccgctccg cggagatgtc
     4261 gagccgggcc agcaccgcgg ggacggcgat gctcggcagg aagtgttcga acatcacgat
     4321 gatgcggcgc tccaggtcct gccgctggca gcgcaactgg gacagcagct gcacccccga
     4381 gaagctgccg accagcagct ccgccgtctc ggccacgtcg acatgcggca gcagttcgcc
     4441 ctgcgccttc gcctcggcca gcatctgcac gttctggtcg atccacatcc ggaaggccgg
     4501 agtgcggtcg aggtccatgg ccccctggtc gagggcgagc cccacactgg cgcgaaccag
     4561 cggttcggac cgcagccggc gggccagcac cagcccggag tcgacgagtt cctggagctt
     4621 gctggactgg ggcgggagcg tgatcccgag ctgctcctcg aacaccccga ccgccagctc
     4681 ctccttggaa ccgaagtgga agtacagcgc gcccttggtg acgcccgccc ggtccaggat
     4741 ctccccgatg gtcgccgagg tgtagccacg ttcgtcgaag acggccgcgg ccgcttcgag
     4801 gatggtccgc cgggtgcgga tcgcgcgctc ctgtcgcgcc atcgggtgcc tcctgatctc
     4861 cgtcgatcgc agtgaaatcg ctgtgcggcg ggggcgcgag agcctgtccc agcggtttgt
     4921 ccaacccttt gaaaaaaacc ggctcgtccg tatcttagtc aacgccgacc tcagccaggc
     4981 agccgatcct gggggttctc atgccgcagt cagaaccttt aagcgcaccc acggacgatc
     5041 agaccggaca ggagggccgt ccggcaaaca gaaccgctgc ggccgtcgag aacgcgccac
     5101 aagccgcagc caccgactcc gcagaaacca ccgaagcccc ctcggtcacc tcggtcacca
     5161 aggaactcgc ccaccgcacc acggaagcag acgtcttccc cgtccggtgg cgccgcatct
     5221 ccgaaacccg cttccgcttc accgctcact ggccggccga ccacgccttc ttcgggcccg
     5281 tggacgaccg ccaccaggac ccgatggtcg tgggcgaaac gttgcggcag gcctcgatgg
     5341 tgctcgcgca cgccgagttc ggcgcccccg cggacaccca cttcgtcatg tgggacctga
     5401 cggtccacac cgacccgtcc gcgctcctgc tgtcggacgc ggcacaaccg gtcgagatcg
     5461 acgtcgtgtg ctccgagata cgccgtcgcg gtcgcggcct gcacagcatg cggaccacga
     5521 tggagttccg ccgcgcggga cgcttcgtcg cccgcggcac cggcagcacc ggctgcacct
     5581 cgccgctcgc ctaccgccgg ctgcgggccg cgcagttggc cgaagtgggc accccggttc
     5641 cgctgctgcc cggcatatca ccggcgctgg ccggccgctc ccgcgccgag gacgtcgtcc
     5701 tcgcccccgc cgaccgcccc ggcgtctggc ggctgcgcgt ggacaccggc catccggtgc
     5761 tcttcccgcg gcccaacgac catgtgccgg gcatggtcct gttcgaggcg gcccgccagg
     5821 cagcgaccgc cgcctccggt cggcaccctt tcctgccccg ctcgatggcc gcgaccttct
     5881 cccggtacgc ggagctgcac agcccgtgct ggatcgagac ggaggtgctc gacgaggggc
     5941 cgggagatcc cggagagacg accgtacggg tctcggggcg gcaggacggc gagtccgtct
     6001 tcagcgccac gctgacctgc ccgctaccgg cccgggtacg gtccctgtcg tgacggtcga
     6061 ccttctgatc acgggcacca gcggcttcgt cggcagcagg gtcgccgccg ccgcagaacg
     6121 gcaacccggc gtccgggtcc ggcacttgtc acgccgtacc ccaccggagg ccggccccgg
     6181 cgcgttcgtc cacggcgacc tcggcgaccc gccctccctg cgcggcagct gcgcgggcac
     6241 cgacgtcctg gtgcactgcg ccacccggat cggcggcgac gccgggaccg tcgaggccat
     6301 gaacgacctg ggcacacgag ccctggtcga ggaggccgtg cgcgccgggg tgagccggat
     6361 cgtgtacgtg agcacggccg ccgtccacgg ccggggcccc ttccgcggtg tacgccccgg
     6421 ggaggtgccg atcgcgcccg cgtcggccac cagccgcacc cgggccgccg ccgaacggca
     6481 cgtcctcgac gcgggcggtc tggtgctgcg cccgcacctc gtgtacggcg agggcgaccg
     6541 gtgggccgtc cccggactgg tgtggctgct gggcgagttg tcgacgacgg ccctcaccgg
     6601 ctgtgcggcg ctccagtcga tgatcgacgt cgacagcctg ggccgtgcgg tggtggccgc
     6661 cgccctgtcc ccggcgcacg acggcggtgt gcaccacgtc aaccaccccg agccggtgag
     6721 cgtcccggac ctgctcgggg ccgtgggcac gctgctggag gagcccggcg gcggcgcggc
     6781 ggtggacgtc gccaccgccc tggaccgggc cgccgggaag ccgctggccc tccatcacct
     6841 cggcatgctc acggtcgacc actggttcgt ggacgacgcc ttctggaagg acctcgactg
     6901 ctcgcccggc gaggggttcg cggccgagtt cagacgcgcg gcaccctggt accgctcgtt
     6961 cctccgaggc cggagagctt cctgatcgcc tccgcctcct ccggacgcgg acccggctgc
     7021 gaaccggcac cgcggccgtg cagcagcacg ggcaactcct ggtgccccgt ggagatgatg
     7081 gaccgcagcc gctgcggggg ccgggacgga tcggccaggg ccagccgcgg gaaccggtcg
     7141 aacagggacg tcagagccac cgtcgcctcg gccatggcga ggggccggcc caggcagtgg
     7201 tgcacgccgt ggccgaagga cacgtggtcg cggctggtgg accgggtgac gtcgaaccgt
     7261 tcggcgtccg ggccgtgccg ctcggggtcg cgtccggcac cggcgaacga gaccacgacc
     7321 gggtcgccct tcgggatcgt cagacctggc tcgatctcga tgtcctcgac ggcgtagcgc
     7381 agtcccgaca tggcaccggg cgcctcgaag cgcagcgact cctcgatggc gtcctcccag
     7441 gtcacctgcc cggtgcggac gagttcgagc tggtcggggt gggcgagcag gctgtgcacg
     7501 gtgttgtcga tgaggttgac ggtcgtctcg aagcccgcgg tgagcagcag gatcaggttg
     7561 tccatcaact cctgttcgct gaggctgcct tcgtcgtgcg cggccatcag ggccgtcgtc
     7621 agatcgtcgg ccgggtgctc gcgcttgtac tggaggaacg cggtcagcgt gccgtacagc
     7681 tcgaccccgt tggcctgggc gtcctccagg gtgatcgccg tgtcgaagaa gcggttgatg
     7741 atgcggtaga gggcgctccg cgggccgtct tcccccggcg gcacgccgaa gagttcgcac
     7801 accacctcat gcggtatcgg cgccgcgaac cccgcccgca ggtcgacgac ttcgccctcc
     7861 gggatccgct ccaggtcgtc cagcgcccgg tcgacgatct cctggacgcg cggcagcagc
     7921 gcgtcgacac ggcgcttggt gaaggcggcg gccatcggcc tgcgcagccg gctgtggtcg
     7981 gtgccgtagg ccgtgaccat gttgcgcacc gacacccaga tcgccagcgg ccaggtgcgg
     8041 gagatgtccc cgttgatcca ggccggccag tgctggaagg cgtccttgct cgtctcgggg
     8101 ccggccagga gccgtttgtt cagctccagg ccgttgatcc accacgcccg tacgccgccc
     8161 gggagttcca cctccacggc cggtccctgg gcccggatcc gggctatctc gccgtagaga
     8221 tcctggccgg acgggtcgat cgccaggaag gggcaggcgt gctgtgccat gactcatcgc
     8281 tcctcggtcc agaggctggg gaaccatcgt gcgatccaag tgacgaaaaa acttggcatc
     8341 cggtttgttt tggccaccac ttcacccgct gtcggccatg ggaggggccg accggcgccc
     8401 gccgggacgc cctgctaccg cagcgcgagt cccgcccggc ccggctcctc cagcgccccg
     8461 tccgccgtgt ccgcctccct gccgacgtcc gcctccccgt cggcgtgcgg gtccgcgtgg
     8521 tccggcccgg tcccggccgg gtccagtgcg ccggccaggt gtggtgcggc cacgcacggc
     8581 aggagcaggt gccacaggtc ggtcagcgag gggcgggcga gccagccggg gtcctcccgg
     8641 gccagcacct cgatgccggt cgtggcggcg aggaccacgg cgctggagcg gtggggcggc
     8701 atcccggcgg cgagttcgcc cctgcgctcc gcctcggcca gcagctcgtg cacgcactcc
     8761 cgccactccc ggcgcaggtc acgcacccgc ccggcgaccg cctcggcgtt gagccgcagc
     8821 cccgcgcgca ccacgatgtc ggcgcacagc agccgggcga cgtggtgcgt gacgtccacg
     8881 aggcgctgca gggcgcaccc gtcgccgccg cggccccggg ccgcgacccg gcgcagcgca
     8941 cgggcggccg cgcgctcgac ggcttcggcc acggcggcct tgttcgcgaa gtggaagtgc
     9001 agcgcgcccg agctgacgcc ggctcccgcg ctgatctccg caagactggc ccggatgtat
     9061 ccccgcgcct cgaactgctc ggccgccgac cggatcagcg cgagacgggt ccggacggcc
     9121 cggtcctgtt tggtcacccc tggctcccgt ggagggctct gatggcgacg caatgaaacc
     9181 aactcgctgg tttttgcaca acgcgctccc gggccgaggg ggttcggtcc ggccgtcacc
     9241 gcctgccgcc cccgccggga gcgggtcggc cgggcccggc cgacccgctc ctacgcccgc
     9301 aagtacgcca gcacggccag cacccggcga tgcgtctcgt ccgccggtgg caggtcaagc
     9361 ttggtgagga tgccggagat gtgcttgccc acggccgcct cggagacgac cagttcgcgc
     9421 gcgatcgcgc cgttggacct gccctccgcg atcagcgcca gcacctcccg ctcacgtggg
     9481 gtgagccgct ccagcgggtc gcggcggcgg cgcagcaact gccgtacgac ctcggggtcg
     9541 acgaccgtgc cgccgtcggc cacctcgccg agcgcctcga cgaactcctc gacctgaccg
     9601 acccggtcct tgagcagata gccgacgccc gtgccgtcac cggagtccag cagctcggcg
     9661 gcgtaccggc gctgcacgta ctggctgagg accaggacgg gcagcgtggg ccgctcctgg
     9721 cgcagccgta ccgccgcgtg caggccctcg tcctggaagc cgggcggcat ccgcacgtcc
     9781 gtcacgacca cgtcgggcgc gtgctccccg acggccgcga tcagtgcccc ggcgtccccg
     9841 acggccgcga cgacctcgtg tccgcagcgg ccgagcagcc cgacgagccc gtcccgcagc
     9901 agcacgctgt cctcggccag gaccacccgc agcggtcgct caactcgcag ggtcacaagg
     9961 gatctccaca cgcagcaggg tcggtccgcc gggcggactg gacagggaga gtctgccatc
    10021 gagcaccgac acccggtcgg cgagtccggt cagcccgctg ccctccccgg cccgcgcacc
    10081 accgcgcccg tcgtcgcgga tcgacaggac cagccggccg ctgcggtacc cgccgctcac
    10141 ttccgcccgg ctcgccccgc tgtgcctgcc cacgttggcg agcgcctcgc acaccacgaa
    10201 gtacgcggcg gcctcgacgg cctgggagaa ccgcccgggc agctgcagat cgacgtccac
    10261 gggcaccgcc gagcggtcgg cggcgtcggc gacggcggct tcgaggccgt agtcggcgag
    10321 gaccttgggg tggatgccgt ggatgagttc gcgcagttcc tcaagggccg tgcccgcctg
    10381 agcatgggcc ttggcgagct gctcggccag cggacccggc ggggcgtcca gccgggccag
    10441 gccgagcgtc atggtcaggg cgacgagccg ctgctgcgcc ccgtcgtgca gatcgcgctc
    10501 gatccgccgc cgttccgcct cgaacgcgtc caccaaccgc acccgggacc gggccagttc
    10561 gaccaccctt gcccctaggt cggcctcgcg cggcgcgacc agcagccggg ccagcccggc
    10621 ccgcgcaccg gcggcgacgc ccagcaggta ggcgcccaga ccgagcagga ccagcccgag
    10681 gacggcacac ccgacggcag cgggccaggt ggtgaccagc cacagcttga ggaccttcgc
    10741 ctcgcccaag acggccatga gcacgggagt cgccaccacg gagagcggtc ccagcaggaa
    10801 gacggcgaac gcgagggcgt cgaccggcca caacagcccc gcgaacagca gggcgtggcc
    10861 cagctcccgc caggtggccc gctcccgcaa ccgggtcgac agccaggccc gcagccccgg
    10921 ctcccggggc gcctcgtgcg ggtcggacgc cggctccgcg tcgaccagcc gcagccggaa
    10981 ccgctccacc gccgccaccg gaatgcccgc cagggccgtc ccgagcagca ggggcagccc
    11041 caccagcgcg agg
//
