LOCUS       NZ_LT907981            11932 bp    DNA     circular CON 12-FEB-2024
DEFINITION  Streptomyces sp. 2323.1 chromosome I.
ACCESSION   NZ_LT907981
VERSION     NZ_LT907981.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. 2323.1
  ORGANISM  Streptomyces sp. 2323.1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2017) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 03:31:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,058
            CDSs (total)                      :: 6,971
            Genes (coding)                    :: 6,808
            CDSs (with protein)               :: 6,808
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 64 of 163
            Pseudo Genes (incomplete)         :: 123 of 163
            Pseudo Genes (internal stop)      :: 17 of 163
            Pseudo Genes (multiple problems)  :: 37 of 163
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:07:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7544115
            Orig. end                         :: 7556047
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT907981.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11932
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="34"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11932
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11932
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6932](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6932
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(1401..1415)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 21.43"
                     /tool="antismash"
     misc_feature    1472..1487
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.76"
                     /tool="antismash"
     gene            1682..2890
                     /locus_tag="CPH93_RS32605"
                     /old_locus_tag="SAMN06272775_6625"
     CDS             1682..2890
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606749.1"
                     /locus_tag="CPH93_RS32605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_6625"
                     /product="MFS transporter"
                     /protein_id="WP_096214903.1"
                     /transl_table=11
                     /translation="MRLFWCAGTCDALGSQMSGLVLPLLLLSLGRTPAEVGAVAGVCAA
                     ATLLLGPFAAVPADRGARKKVMVGAALVSAAAMTGVALAVRSGTVPMAVLIGAVLVERS
                     ATSCYEAASLGTVALVCPPAEHRRVLSRLQAGERGALVLGPALGGLLFQAGRWLPFLAD
                     ALSYVVAAGCIRALRTELSPPREQRPPAGPRRNWRVAAAEAGAGVALIRREPLLRLALG
                     WLSAVNGLLAALYYTTVFALQDQGRGAAGPGLVLALAGAAGLAGALLAPRLAGRRSPTR
                     VLVAVSWLMLPPAAALAAARDAWQFGLLFGLLCLLTPLATVALQARIIQVIPPGLQART
                     GTVLATASGAAAALAPALAGLAAGRVGPGTTLVGCAALLAAVALHATWAAPALTRGTGE
                     EPA"
     misc_feature    2078..2080
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    2813..2815
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2887..4752
                     /locus_tag="CPH93_RS32610"
                     /old_locus_tag="SAMN06272775_6626"
     CDS             2887..4752
                     /NRPS_PKS="Domain: Abhydrolase_1 (363-504). E-value: 3e-09.
                     Score: 28.8. Matches aSDomain:
                     nrpspksdomains_CPH93_RS32610_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606750.1"
                     /locus_tag="CPH93_RS32610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_6626"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_100315494.1"
                     /sec_met_domain="PF00561 (E-value: 1.1e-05, bitscore: 23.8,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S9 (E-value: 2e-36, bitscore:
                     124.1, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPHAARPAAAPSPGTGFRVYRPALPEVCPRDPARMALAADAGGR
                     CEIFTWHAATGTARQVTDSPDGTLHCALDADARVWWFAENRRGAGRWYFQEFDGGPRQP
                     GLTGLPSGLPHGLAVSHAGTVAIGLGDGHGMTVHLGMPGGPARAVHTVDGHALLAGISP
                     SGGLLALSGAAGSDRAVTLLTCSGALLDQLSGRTGRLWALGFAPSPAGTELLLVQEADG
                     HYRLATWRPGSGLRAQDWCRFDTEITARWYPEGRRVLVRQDRHGRSTLAAADLDRRTLT
                     PVPSPPGTLLDAAPHPGDDVHYLWTDAATPPQMRSTAGTRLPALATLPARVPGRHRELW
                     TPGPDGPVHTLLSEPDSAPGRRPPLVFLVHGGPADHDRDAYDPEVHSLVASGFAVARVN
                     YRGSTGYGPRWRTAFGAGVGLTQVADLVAVRSDLLRRGLAREDAIGLWGTSWGGYLALL
                     ALGTGPDLWQAGVAVKPVADCVAAHRTGTPALRALDERLFGGTPDDVPERYARSSPLHY
                     AAEVRAPLLVIAATLDAKCPPGQVRGYLTALREAGVPHESLWLETGHDGYSGAHHMAVL
                     QRAMGFLDQHLRRAPAAPRTPRSAADGVFGGTGPSGAPECTTHVP"
     aSDomain        3976..4398
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CPH93_RS32610_Abhydrolase_1.1"
                     /evalue="3.00E-09"
                     /label="CPH93_RS32610_Abhydrolase_1.1"
                     /locus_tag="CPH93_RS32610"
                     /protein_end="504"
                     /protein_start="363"
                     /score="28.8"
                     /tool="antismash"
                     /translation="PLVFLVHGGPADHDRDAYDPEVHSLVASGFAVARVNYRGSTGYGP
                     RWRTAFGAGVGLTQVADLVAVRSDLLRRGLAREDAIGLWGTSWGGYLALLALGTGPDLW
                     QAGVAVKPVADCVAAHRTGTPALRALDERLFGGTPDD"
     gene            5001..6932
                     /locus_tag="CPH93_RS32615"
                     /old_locus_tag="SAMN06272775_6628"
     CDS             5001..6932
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606751.1"
                     /locus_tag="CPH93_RS32615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_6628"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_093481682.1"
                     /sec_met_domain="TIGR03975 (E-value: 4.4e-183, bitscore:
                     608.6, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLVNMPWSPIDLPSLALGILRRSVDERTSGHADVLHANLEFT
                     DWITRRTAFTGDDYQFYALSSYFMGCGDWVFSSALYDDPEWRAEEFSASMRGKLRAERL
                     QMSHELHRVVPEFVQEIATRIVAAGPDVVGFTSTFQQNTAALAAAKYVKRLAPHIKTVM
                     GGANCDAEQGAAAHRNFGFLDFVVRGEGEAAFPQLLTALDEGTELSAVPGLCHRQADGT
                     STANPMSTSPLPPATILPPDYSGYFERLASSVARNWVEPKLVVEGARGCWWGEKHHCTF
                     CGLNGSFMQFRSKSPDTFLEEIMDLARRHRVLDMYLVDNILDMGYLTTVLPRIIDSGYD
                     LRMHIEIKANMRRAQLRTLAEAGMIYVQPGIESLNSRVLDLMDKGVSGCQNVRMLRDGA
                     ETGLSVCWNYLHGFPGETAADYEPVIAQIPALEHLDPPVDLSARIAIERFSPYFNRPEL
                     GFTGLRPEEHYRFTYDLPESELLDLAYVFEAPERGITEPTVTALNDALGAWKKHHADAR
                     LTHADLGDRIVLVSRRRAFDWGAMELTEPLETAAFRLLDQPHAPAALIRKLAARLPAHP
                     VDATAVHALLQHWVTLGLVFTDGGQYVHLAPAAVNEDLLRLDYMRHTHAAPAPQEQPDD
                     AARAVAHV"
     gene            6955..7623
                     /locus_tag="CPH93_RS32620"
                     /old_locus_tag="SAMN06272775_6629"
     CDS             6955..7623
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606752.1"
                     /locus_tag="CPH93_RS32620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_6629"
                     /product="DUF5825 family protein"
                     /protein_id="WP_093481681.1"
                     /transl_table=11
                     /translation="MHRTATPTGPTLSAWRDYDEAACALPGMSLGTLIPAGPPTEQADR
                     LWELGVRRVRFAEEIDLTAVDELGAAAHAVQRLCLIRDLTARAVLVQWQLRLPAEPDDG
                     WRDLSHLQPPGTLTGPADPAAALAQWRREHYLCKCLWRQGPGFVQIRDRRWGELRRFTA
                     DEPAYREAIDKLAYGAPQSDVPAAITADFLAERLIARTGPLLWWLPYRVNRWLQEAMAI
                     "
     gene            complement(7654..9447)
                     /locus_tag="CPH93_RS32625"
                     /old_locus_tag="SAMN06272775_6630"
     CDS             complement(7654..9447)
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606753.1"
                     /locus_tag="CPH93_RS32625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_6630"
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="WP_096214904.1"
                     /transl_table=11
                     /translation="MEPRTPDHGPGRLPAGSGGRPAEGARGEAERGPEAQTDRLRYLES
                     ATRRIAWGSDRDETLGELCRAAIPAFADAILVHLRDPLPSGEAEPAEPSVLRLHSMAQG
                     PEASVTEAPAGAAEFAPLTVSGRFVGILLAGRPVFGDAPGLVPTVTALLEPVASLPATT
                     APGRRLIVAPLYGRRHVLGTVALLRGAGRPAFTEDDLLVASQLATHAALGIDKAKVSGH
                     EASMADALQLTMLPSSLPRTTGVRLASRYLPAADTAQVGGDWYDAIPLPGNRVALVVGD
                     VLGHSMTSAAIMGQLRTTVQTLAGLDLPPEEVLHHLDEQAQRLGSEHMATCLYAIYDPI
                     AQRLLIADAGHPPPVLLHPDGFGEVLQIPPGAPIGVGGVPFESVEMPAPTGATLLLYTD
                     GLVESRERDVWAGVELLRARLQAAADAFGSPPLDMLCDAVLNILEPESLDDDVALLAAR
                     FDGFPPLNVAYWFLSPQPQTAGRARQLTRRALHRWGLDSMLDATELMVSEVVTNAVRYA
                     SRPIALRLLRTDVLRCEVGDDSPQVPRMRRAQAGDEGGRGLFLVDQLAQRWGATRLSTG
                     KVVWFEQQMPEERRQKTPSAP"
     gene            complement(9512..10213)
                     /locus_tag="CPH93_RS32630"
                     /old_locus_tag="SAMN06272775_6631"
     CDS             complement(9512..10213)
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606754.1"
                     /locus_tag="CPH93_RS32630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_6631"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_162501018.1"
                     /sec_met_domain="PCMT (E-value: 5.1e-06, bitscore: 25.0,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDGFLETGYGSDETGGEDVRPDREGQAEAFDAIGDRYDEAFPFK
                     EGQLAAGEWLIRSLPGGSRVLDLGCGTGLPTARQLVDAGCEVVGVDLSRGMVELARKYV
                     PDATFHRLDIADLAPGGPHDLGRFDAVAAFFSLLMLPRAEIPLALHTIHDLLVPGGLFA
                     LAMVEADVDYFTMPFLGNTIRVSGYLREDLRKVIEAADFGVVEETSYTYAPAISDVPPE
                     EQLFLRCRRRD"
     gene            complement(10387..11244)
                     /locus_tag="CPH93_RS32635"
                     /old_locus_tag="SAMN06272775_6632"
     CDS             complement(10387..11244)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088796.1"
                     /locus_tag="CPH93_RS32635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_6632"
                     /product="RNA polymerase sigma factor SigF"
                     /protein_id="WP_093488367.1"
                     /transl_table=11
                     /translation="MTTVVNDTTTEQHSDLAAELPEVECPSAVAPRDARELSKQFFDRL
                     QFLDEGTHEYQYARNTLIEMNMSMVRYVARRYRNRGDDMEDIIQVGTIGLIKAIDRFDL
                     SREVEFSTFAVPYILGEIKRFFRDTGWAVHVPRRLQELRSDLAKAKEELHVQLDRDPTV
                     QELAAHLDLSEDEVIEGIVAANGYCAGSLDTPTDPDPSHQRRALADVLGEEDPAMGTVE
                     DLHALAPLLQELDPRERQLIELRFGQEMTQSQIGAELGVSQMHVSRLLTRTLTKLRAGM
                     LSDR"
ORIGIN
        1 cctggacgtg gaggtcacgg cccaggaact ggtcgacgca gcggtgccgg acttctgcga
       61 cggagccgtc gtggagctcg tggactggcg gaacgagagc gagtcgttcg acccggcgct
      121 gcccgtggac acccggcgga tcgcctccgg caccgtactg cccgctcccg cgacggagct
      181 ggtcagcggg ctgaaagtgg tgcactaccc accgggctcc gcgatccacc agatgctgcg
      241 gtccggccgt ccgatctgcg tgccggtgaa cgacgacttc ctggcgcgga cggtgctgga
      301 cgcctcgcgg gcccggctga tggccgacag cgggctggtc gccctcctcc tcgccccgct
      361 catcgcccgg ggaacggtcc agggactcgc catcttcggc cggtccgccc gccgggcgga
      421 cttcaccgag caggacctca gcctggccgg agagctcgcc tcgcgtgccg cgctgtgcct
      481 ggacaacgcg cggctgtaca accgggagcg gaacatcggg ctcaccctcc aacgggccct
      541 gctgcccagc tatctggcgc ccagcccgta tctgcacctc gcgcaccgct atctgcccgg
      601 cagccgcgtc accgaggtcg gcggcgactg gtacgacgtg atcgcgctgc ccgaccaccg
      661 ggtcgccctg gtcgtcggtg acgtcatggg acacggggtc tcggccgccg catcgatggg
      721 ccggctgcgg atcacggcca aggcgctggc ccggcacgac caggaaccgg ccgagctgct
      781 cggcgaactc gaccagtgcg cgctggaggc cggtatcgag ctggcgacct gcctctacat
      841 ccgctacgac cccctcaccg gccgcacccg gctcgccagc gccggtcatc cgccgcccct
      901 ggtccggcac cccgacggcc ggatcgaggt catcgacgag gtgctgggcg tccccctcgg
      961 gatcggcggc accaccttcc gcacggcgga gatcggcctg cccgacgacg ccgtcctcgc
     1021 cctgtacacc gacggcctca tcgaggcgcg gggccgcgat atcgacgagg gtctgcacca
     1081 gctccaggag gaactggccc ggatccccgg ctcgttggag gtcgcggccg accgcatcct
     1141 ctcccgtctc gtggcgagct ctccggccga tgacaccgtc ctgctcctcg cccgcgtgca
     1201 ccgccggcgg taggggggcg cgtgggggga gagggggcgg cgggtggtgc gcgacggccg
     1261 gctcgcccgt cccggccggc ggccggctcg ccgtcccggc cggtgtccgc tgaccgtccc
     1321 ggccggtgtc cgctgaccgt cccggccggt gtccgctgac tgttccgggc cggtgacatg
     1381 ccgcccgaag gtgttcatgg accgacatct tgtggcctac gtcaccgcct ggttaagctc
     1441 cggcacactt cgaacacatt tcccccgcaa gttgaccttt tggtgaggtc gatcttcaag
     1501 gtcgacccac ggatacgagg tggcttcgca tggaacggcg cacgtcggca accccggcgg
     1561 atgactcacc gcgcggctta cgcccggcgt cacccgcgag accggccccg ggccgtaccc
     1621 ggtgaccggt tccgccccgg cgacggcatc cgccgttccg ccggcccaag tgcgccgcga
     1681 catgcggctg ttctggtgcg cgggcacctg tgacgcgctg ggcagccaga tgtccgggct
     1741 cgtcctgccg ctgttgctgc tgagcctggg ccggaccccc gccgaggtcg gggccgtcgc
     1801 cggggtctgc gcggccgcca cgctgctcct cggccccttc gccgcggtac ccgccgaccg
     1861 cggggcgcgc aagaaggtga tggtgggcgc cgcgctggtg tccgccgccg cgatgaccgg
     1921 cgtcgcgctg gccgtccggt ccggcacggt cccgatggcc gtactcatcg gcgccgtact
     1981 cgtcgagcgg tccgcgacct cctgctacga ggcggcgtcg ctcggcaccg tcgccctggt
     2041 gtgtccgccg gcggaacacc ggcgggtgct gtcccgatta caggcggggg agcggggggc
     2101 cctggtcctc gggccggccc tgggcgggct gctcttccag gccggacgct ggctgccgtt
     2161 cctcgccgac gccctctcgt acgtcgtcgc ggcgggctgc atccgcgccc tgcgtaccga
     2221 gttgtccccg ccccgggaac agcggccgcc ggccggcccg cgccggaact ggcgcgtggc
     2281 ggccgcggag gccggggccg gggtggcgct gatccggcgc gagcccctcc tgcggctggc
     2341 gctcgggtgg ctctcggccg tcaacgggct gctcgccgcg ctgtattaca cgacggtctt
     2401 cgccctgcag gaccagggcc gcggcgcggc cgggccgggt ctcgtcctcg cgctcgccgg
     2461 ggcggccggg ctggccggcg cgctgctcgc gccccgactg gccggccggc gctccccgac
     2521 gcgcgtgctg gtggcggtgt cctggctgat gctgccgccc gccgcggccc tggcggcggc
     2581 ccgcgacgcc tggcaattcg gcctgctctt cggcctgttg tgtctgctca cgccgctggc
     2641 caccgtggcg ctgcaggccc gcatcatcca ggtcataccg cccgggctcc aggcccgcac
     2701 cggcaccgtg ctggccaccg cgtccggcgc ggccgcggcc ctggcaccgg ccctcgccgg
     2761 gctggccgcc gggcgggtcg gacccgggac caccctcgtg ggctgcgccg ccttactggc
     2821 cgcggtcgcg ctccatgcga cctgggcggc accggccctc acccgcggca cgggggagga
     2881 gcccgcatga gcccgcacgc ggcacgcccc gccgcagccc cgtcccccgg caccggcttc
     2941 cgggtctacc ggccggcgct ccccgaggtc tgcccgcgcg acccggcacg catggccctg
     3001 gccgcggacg ccggcggccg ctgcgagatc ttcacctggc acgctgccac cggcaccgca
     3061 cgccaggtca ccgacagccc ggacggcacc ctgcactgcg ccctcgacgc cgacgcccgg
     3121 gtgtggtggt tcgccgagaa ccggcgcggc gccgggcgct ggtacttcca ggagttcgac
     3181 ggcggaccgc ggcagcccgg cctgaccggt ctcccctcgg gcctgccgca cggactcgcc
     3241 gtcagccacg ccgggacggt ggccatcggc ctcggcgacg gccacggcat gaccgtgcac
     3301 ctcggcatgc cgggcggccc ggcccgggcg gtgcacaccg tcgacggcca tgcgctgctg
     3361 gcgggcatct cgccgtccgg cgggctgctg gccctgtcgg gcgcggccgg ctccgaccgg
     3421 gcggtcaccc tcctcacctg ctccggggcc ctgctcgacc agctctccgg acgcaccggc
     3481 cggctgtggg cgctcggctt cgcaccctcc ccggcaggca ccgaattgct gctggtccag
     3541 gaggccgacg gccactaccg gctggccacc tggcggcccg gctcggggct gcgggcccag
     3601 gactggtgcc ggttcgacac cgagatcacc gcgcgctggt accccgaagg ccgccgggtc
     3661 ctcgtccgcc aggaccgcca cggccgctcc accctcgcgg ccgccgacct cgaccggcgc
     3721 accctgaccc cggtgccctc gccgcccggc accctgctcg atgccgcccc gcacccgggc
     3781 gacgacgtgc actacctctg gaccgacgcg gccaccccgc cgcagatgcg ctccaccgcc
     3841 ggcacccgcc tcccggcgct cgccaccctg cccgcccggg tacccggccg gcaccgcgag
     3901 ctgtggaccc ccggcccgga cgggcccgtg cacaccctgc tcagcgaacc ggactcggcc
     3961 cccggccgac ggcctccgct ggtgttcctg gtgcacggcg gtcccgccga ccacgaccgc
     4021 gacgcctacg acccggaggt gcactccctg gtcgcctccg ggttcgcggt cgcccgggtc
     4081 aactaccgcg gctccaccgg ctacgggccc cgctggcgca ccgcgttcgg cgcgggcgtc
     4141 gggctcaccc aggtcgccga cctcgtcgcg gtccgttccg atctgctgcg ccgcggcctg
     4201 gcccgcgagg acgcgatcgg tctgtggggc acctcctggg gcggctacct ggcgctgctg
     4261 gcgctcggca ccggcccgga cctgtggcag gcgggggtcg ccgtcaaacc ggtcgccgac
     4321 tgcgtggcgg cccaccgtac gggcaccccc gcgctgcggg ccctggacga gcggctgttc
     4381 ggcggcaccc ccgacgacgt acccgaacgc tacgcgcgca gctcacccct ccactacgcc
     4441 gccgaggtcc gcgccccgct gctggtgatc gccgccaccc tcgacgccaa atgcccgccc
     4501 ggccaggtac gcggctacct caccgccctg cgggaggcgg gtgtgccgca cgagtccctg
     4561 tggctggaga ccggccacga cggatacagc ggcgcacacc acatggccgt actgcagcgc
     4621 gccatgggct tcctggacca gcatctgcgc cgcgcccccg cggcccctcg aactccccgt
     4681 tccgcagcgg acggggtctt cgggggcacc ggcccatccg gtgcccccga gtgcacgacg
     4741 cacgttccat aactcgccag aacaggagaa cagccatgca gaaggacatc atccacaacg
     4801 acccgctcac gggtgacgaa gagaaccgcc ggccgaccgt cggcatcacg gtgaccgtcc
     4861 cgttccgcaa cgcggaggac accgaggagg actgaccctc cccggccggc ccggcaccac
     4921 cgggccggcc ggacccgtgc accgcaccgg ccggccgccg gccgcccgcc aacccttccg
     4981 ccgccgtacg tggagccgat atgcgcgtcc tgctggtcaa tatgccctgg tccccgatcg
     5041 atctgccctc gctcgcactc ggaatcctca gacgaagcgt cgacgagcgc acctccgggc
     5101 acgccgatgt cctgcacgcc aacctggagt tcaccgactg gatcacccgg cgtaccgcct
     5161 tcaccgggga cgactaccag ttctatgcgc tctcctcgta cttcatggga tgcggcgact
     5221 gggtgttctc ctccgcgctc tacgacgatc ccgagtggcg cgccgaggag ttcagtgcct
     5281 cgatgcgcgg caaactgcgc gcggaacggt tgcagatgtc ccatgaactg caccgcgtgg
     5341 taccggagtt cgtccaggag atcgccactc ggatcgtcgc ggccggcccc gacgtcgtcg
     5401 gcttcacctc caccttccag cagaacaccg cggcgctggc cgccgccaaa tacgtcaagc
     5461 gcctcgcccc gcacatcaag accgtcatgg gcggcgccaa ctgcgacgcc gagcagggcg
     5521 ccgccgccca ccgcaacttc ggcttcctgg acttcgtggt ccgcggtgag ggcgaggccg
     5581 ccttcccgca gctcctcacc gccctcgacg agggcaccga gctgtccgcc gtccccgggc
     5641 tgtgccaccg gcaggccgac ggcaccagca ccgccaaccc gatgagcacc agcccgctgc
     5701 cgcccgccac catcctcccg cccgactaca gcggctactt cgagcggctg gcgtcctccg
     5761 tggcgcgcaa ctgggtggag cccaagctcg tcgtcgaggg ggcccgcggc tgctggtggg
     5821 gcgagaagca ccactgcacc ttctgcggcc tcaacggctc cttcatgcag ttccgcagca
     5881 agagccccga caccttcctc gaagagatca tggacctggc ccgccgccac cgggtgctgg
     5941 acatgtacct cgtcgacaac atcctcgaca tgggctacct caccaccgtc ctgccccgca
     6001 tcatcgacag cggctacgac ctgcggatgc acatcgagat caaggcgaac atgcgccggg
     6061 cccagctgcg caccctcgcc gaggccggaa tgatctacgt ccagccgggc atcgagagcc
     6121 tgaacagccg ggtgctcgac ctgatggaca agggcgtgag cggctgtcag aacgtccgga
     6181 tgctccgcga cggagccgag accggactct cggtctgctg gaactacctc cacggcttcc
     6241 ccggtgagac cgccgccgac tacgagccgg tgatcgccca gataccggcc ctggaacacc
     6301 tcgacccgcc ggtcgacctg tccgcccgca tcgcgatcga acgcttcagc ccgtacttca
     6361 accggcccga actcggcttc accggcctgc gccccgagga gcactaccgc ttcacctacg
     6421 acctgcccga gtccgaactg ctcgatctgg cctatgtctt cgaggcaccc gaacgcggca
     6481 tcaccgagcc caccgtcacc gcgctcaacg acgccctcgg cgcctggaag aagcaccacg
     6541 cggacgcccg gctcacccac gccgacctcg gcgaccgcat cgtgctggtc agccgccggc
     6601 gcgccttcga ctggggcgcc atggaactca ccgagccgct ggagaccgcg gccttccggc
     6661 tcctcgacca gccgcacgcc cccgccgccc tgatccgcaa gctcgcggcg cggctgccgg
     6721 cccaccccgt cgacgcgacc gccgtccacg ccctgctcca gcactgggtg acgctcggcc
     6781 tggtcttcac cgacggcggc cagtacgtcc atctggcacc ggcggccgtc aacgaggacc
     6841 tgctgcggct cgactacatg cgccacaccc atgccgcccc cgccccgcag gaacagccgg
     6901 acgacgccgc ccgcgccgtc gcccacgtct gacgccggtc cacgaggaga caccatgcac
     6961 cgcaccgcca cccccaccgg gccgacgctc agcgcctggc gggactacga cgaagccgcc
     7021 tgcgcactgc ccggcatgag cctgggcacc ctcattccgg ccggcccgcc gacggaacag
     7081 gccgaccggc tgtgggaact cggggtacgc cgggtgcggt tcgccgagga gatcgacctg
     7141 accgcggtcg acgagctcgg cgccgccgcg cacgccgtcc aacggctctg cctgatccgc
     7201 gacttgacgg cccgcgcggt cctggtgcag tggcagctgc gcctgcccgc ggagcccgac
     7261 gacggctggc gcgacctcag ccacctccag ccgcccggga ccctcaccgg ccccgccgac
     7321 ccggcggccg cgctcgccca gtggcgcagg gagcactacc tctgcaaatg cctgtggcgc
     7381 cagggcccgg ggttcgtcca gatccgcgac cgccggtggg gcgagctgcg ccgcttcacc
     7441 gcggacgagc ccgcataccg ggaggcgatc gacaaactgg cctacggcgc cccgcagtcg
     7501 gacgtgcccg ccgccatcac cgccgacttc ctggcggaac ggctgatcgc ccgcaccgga
     7561 ccgctgttgt ggtggctgcc ctaccgggtg aaccggtggc tccaggaggc gatggccatc
     7621 tgaacccggg ccgggcgccg ccgcgggcca cagtcagggc gcgctgggcg tcttctggcg
     7681 gcgctcctcg ggcatctgct gctcgaacca gacgaccttg cccgtgctca accgcgtcgc
     7741 cccccagcgc tgggccagct ggtcgaccag gaacaggccg cgcccgccct cgtcacccgc
     7801 ctgcgcgcgc cgcatccggg gcacctgcgg tgaatcgtcg cccacctcgc accgcagcac
     7861 atcggtgcgc agcagccgca gcgcgatcgg ccgggaggcg tagcgcaccg cgttcgtcac
     7921 cacctcgctg accatgagct cggtcgcgtc gagcatcgag tccagccccc agcggtgcag
     7981 tgcccggcgg gtcaactgcc gtgcccggcc cgccgtttgg ggctgcgggc tgaggaacca
     8041 gtacgcgaca ttgagcggcg ggaacccgtc gaaccgggcg gcgagcagcg cgacatcgtc
     8101 gtccaggctc tccggttcca ggatgttcag caccgcgtcg cagagcatgt ccagcggcgg
     8161 ggacccgaac gcgtcggcgg cggcctgcag acgggcgcgc agcagctcca cccccgccca
     8221 gacatcccgc tcccgggact cgaccaggcc gtcggtgtac agcagcaggg tcgctccggt
     8281 gggcgcgggc atctccaccg attcgaaggg gacgccgccc accccgatcg gcgcgccggg
     8341 cgggatctgc agcacctcgc cgaagccgtc cgggtgcagc agcaccgggg gcggatggcc
     8401 cgcgtcggcg atcagcagcc gctgcgctat cgggtcgtag atggcgtaga ggcaggtcgc
     8461 catgtgctcg ctgcccaggc gctgggcctg ctcgtcgagg tgatgcagca cctcctccgg
     8521 cggcaggtcg agcccggcga gggtctgcac ggtggtacgc agctggccca tgatcgcggc
     8581 ggaggtcatg gagtggccca ggacgtcgcc gacgaccagc gcgacgcggt tgccgggtag
     8641 cggaatcgcg tcgtaccagt cgccgcccac ctgggcggtg tcggcagccg gcagataccg
     8701 gctcgccagc cgcacgcccg tggtccgggg cagcgacgag ggcagcatcg tcagctggag
     8761 cgcgtccgcc atggaggcct catggccgga caccttcgcc ttgtcgatgc cgagcgcggc
     8821 atgggtggcc agctgcgagg cgacgagcag atcgtcctcc gtgaaggcgg gccggccggc
     8881 cccgcgcagc agggcgacgg tgcccaggac gtgccggcgg ccgtacagcg gggcgacgat
     8941 cagccgccgg cccggtgcgg tggtcgccgg caggctcgcc accggctcca gcagggcggt
     9001 caccgtgggc acgagcccgg gggcgtcgcc gaacacgggc cgcccggcca gcaggatccc
     9061 gacgaaccgt ccgctcaccg tcagcggtgc gaactcggcg gcccccgcag gcgcctcggt
     9121 gaccgaagcc tccggcccct gggccatgct gtgcagccgc aggacgctcg gttcggccgg
     9181 ctccgcctcc ccgctcggca gcgggtcccg cagatggacg aggatcgcgt cggcgaacgc
     9241 ggggatcgcc gcccggcaca gttcccccag cgtctcgtcc cggtcgctgc cccatgcgat
     9301 ccgccgcgtc gcggattcga ggtaccgcag acggtcggtc tgtgcctcgg ggccacgctc
     9361 cgcttccccc cgggcaccct ccgcagggcg ccctccggat ccggcgggga gcctgccggg
     9421 tccgtggtcg ggggtacggg gctccatcac tcgtgggtgt ccgtcactcg tgggtgtccg
     9481 tccggtgggt gccaggacgt accgggcggc ctcagtcgcg ccgccggcag cgcaggaaga
     9541 gctgctcctc gggcggtacg tccgagatcg ccggggcgta ggtgtacgag gtctcctcga
     9601 cgaccccgaa gtccgccgcc tcgatgacct tgcgcaggtc ctcccgcaga tagccggaca
     9661 cccggatggt gttgccgagg aacggcatcg tgaagtagtc gacgtcggcc tccaccatcg
     9721 cgagcgcgaa caggccaccg gggaccagca ggtcgtggat cgtgtgcagc gcgaggggga
     9781 tctcggcacg cggcagcatc agcagggaga agaacgccgc gaccgcgtcg aagcggccca
     9841 ggtcgtgcgg gccgccgggc gccaggtcgg cgatgtccag ccggtggaac gttgcatcgg
     9901 gcacatattt acgggcgagc tcgaccatcc cacgggacag atcgacgccg accacctcgc
     9961 accccgcgtc caccagctgc cgggcggtcg gaaggccggt gccgcagccg aggtcgagta
    10021 cgcgggagcc gccgggcagc gagcggatca gccactcgcc ggccgcgagc tgtccctcct
    10081 tgaacgggaa cgcctcgtca tagcggtcac cgatcgcatc gaacgcctcg gcctgcccct
    10141 cgcggtcggg ccgtacgtcc tcgccaccgg tctcgtcgct cccgtaaccg gtctccagga
    10201 atccgtcgct cacgaattac gcctcttcac gtgcgtctat tcgattactt ccatcatgca
    10261 ccccaccccg gcccacggcc cgtacggagg gcgtacgggg ctacggtccc ggggcgggcg
    10321 acccaacgac cgcgggcggg cacccggcct gtacggccgg agcccgcccg acgggaacgg
    10381 tcccggtcag cggtcgctga gcatgccggc gcgcagcttg gtcagggtgc gggtgagcag
    10441 acgggagacg tgcatctggg agacgccgag ctcggcgccg atctgggact gggtcatctc
    10501 ctgcccgaag cgcagctcga tcagctggcg ctcccgcggg tcgagctcct gcagcagcgg
    10561 cgcgagggcg tgcaggtcct cgacggtccc catggccgga tcctcttcgc ccaggacatc
    10621 ggcgagggca cgacgctggt gggaggggtc gggatcggtg ggggtgtcca gcgagccggc
    10681 gcagtagccg ttggccgcga cgatcccctc gatcacctcg tcctccgaca ggtccagatg
    10741 ggcggcgagt tcctggaccg tggggtcgcg gtcgagctgg acatggagct cttccttggc
    10801 cttcgccaga tcgctgcgca gctcctgcag ccggcgcggg acgtgcaccg cccagccggt
    10861 gtcgcggaag aagcgcttga tctcgccgag gatgtagggc acggcgaagg tggagaactc
    10921 gacctcacgg gagaggtcga agcggtcgat ggccttgatc agtccgatcg tgccgacctg
    10981 gatgatgtct tccatgtcgt cgccacgatt gcggtagcga cgcgcgacgt agcgcaccat
    11041 cgacatgttc atctcgatga gcgtgttccg cgcgtactgg tactcgtgcg tgccctcgtc
    11101 gaggaactgc agccggtcga agaactgctt cgacagctcc ctcgcgtccc tcggggccac
    11161 cgcgctgggg cactccacct ccggcagttc tgcggccagg tcggagtgct gctcggttgt
    11221 ggtgtcgttc acgacggtgg tcatcatgcc tccctgcgcg tccggcacac gcgaacgcac
    11281 agcggccgcc tacccagcaa gaacactcct atgcgggtga cttgccagtt caacgccctg
    11341 tgacgctcgg acctcgggaa gcgccccgtc tcagacaccg aaatgatcgg ggtcggccgc
    11401 cgcccagtcc gcggcccagt cctgcggcgg gtccgccggc aactcgccgg ggcgcagcca
    11461 gtcgtagagc tcctcgtacg accggacggt gagcggatcg atccgccggt gcagcatcca
    11521 gggccgcagg ccgtccggat tccggacgcc catggagccc atgatctgca gggcgctgtg
    11581 caccgttgcc ttctggaacc tgtgcacgcg cgcggacttg tcaccgacgt ccagggcccg
    11641 gccccggcgc ggatcctggg tcgccactcc gaccgggcag gtattggtgt ggcagcgctg
    11701 tgcctgaata caaccgacgg ccatcatcat ggcgcgtgcc gcattcgtgt aatccgcccc
    11761 ctgcaccagg cgcttgacca tatcggcgcc ggtggcgacc tttccgctcg cgccgatttt
    11821 gatgcggtcc cgcagtccgc agccgaccag tgcgttgtgc acggtgatca ggccttcggt
    11881 cagcggggca ccgagatggt cggcgaattc caggggagcg gccccggtgc cg
//
