LOCUS       NZ_LT907981            10417 bp    DNA     circular CON 12-FEB-2024
DEFINITION  Streptomyces sp. 2323.1 chromosome I.
ACCESSION   NZ_LT907981
VERSION     NZ_LT907981.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. 2323.1
  ORGANISM  Streptomyces sp. 2323.1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2017) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 03:31:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,058
            CDSs (total)                      :: 6,971
            Genes (coding)                    :: 6,808
            CDSs (with protein)               :: 6,808
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 64 of 163
            Pseudo Genes (incomplete)         :: 123 of 163
            Pseudo Genes (internal stop)      :: 17 of 163
            Pseudo Genes (multiple problems)  :: 37 of 163
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:07:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6216599
            Orig. end                         :: 6227016
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT907981.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10417
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="26"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10417
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10417
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5417](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5417)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            32..775
                     /locus_tag="CPH93_RS26760"
                     /old_locus_tag="SAMN06272775_5447"
     CDS             32..775
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: oMT (37-141). E-value: 1.6e-14. Score:
                     44.7. Matches aSDomain: nrpspksdomains_CPH93_RS26760_oMT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578407.1"
                     /locus_tag="CPH93_RS26760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_5447"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_096214295.1"
                     /transl_table=11
                     /translation="MNREQISRIAHAEHPIKSPLDDDSVRRLLERGLPRGDERVLDLGC
                     GGAEWLLRALAMCPHLHAVGVDISEDALAQARRAASDLGVQERLVLHHQEAGDFVSSQP
                     FDLVLSFGATHAFGGLLPTLAAARRHLAPGGRVLIGEGFWDRAPSQEAREMLGDFTDLA
                     TTVDRVVSEGWTPVHGHVSTRRELDDYEWACWGSLASWALDHPADPASSHVLTTATTRR
                     SEWLRAYRDTWGFVSLVLRRTPDCP"
     aSDomain        143..454
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CPH93_RS26760_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="1.60E-14"
                     /label="CPH93_RS26760_oMT.1"
                     /locus_tag="CPH93_RS26760"
                     /protein_end="141"
                     /protein_start="37"
                     /score="44.7"
                     /tool="antismash"
                     /translation="ERVLDLGCGGAEWLLRALAMCPHLHAVGVDISEDALAQARRAASD
                     LGVQERLVLHHQEAGDFVSSQPFDLVLSFGATHAFGGLLPTLAAARRHLAPGGRVLIGE
                     "
     gene            880..1584
                     /locus_tag="CPH93_RS26765"
                     /old_locus_tag="SAMN06272775_5448"
     CDS             880..1584
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606337.1"
                     /locus_tag="CPH93_RS26765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_5448"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_096214296.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYIGKARFEAGLAAFAHREVVEVVHRSFELDP
                     NAPAADGVPMLDMLAAKYGVTREQAAAMEARVAEAAAGEGLDYRSDRIHGNTFDLHRLL
                     HLGKLYGVQDALLDAFYRANFAEARALGDPAVRSEIAVGAGLPADEVARVLADPGAYAQ
                     EVRAEERAAAELGATGVPFFVIDRRYGISGGQPAEVFRQALERAYADGGEPLSTAGEGE
                     AHGDDGCAVPGT"
     misc_feature    complement(1669..1683)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.25"
                     /tool="antismash"
     gene            1699..2760
                     /locus_tag="CPH93_RS26770"
                     /old_locus_tag="SAMN06272775_5449"
     CDS             1699..2760
                     /NRPS_PKS="Domain: Aminotran_5 (16-348). E-value: 7.2e-23.
                     Score: 73.0. Matches aSDomain:
                     nrpspksdomains_CPH93_RS26770_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606338.1"
                     /locus_tag="CPH93_RS26770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_5449"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_093488557.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.7e-16, bitscore:
                     57.7, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQRREPLGGAEFAPETTYLNSASSGLLPARSAAALRAALDESASY
                     GTMGRDYFAAASASREAFARLMGVPAERVAVGSSVAVQSAFVASSLPADAEVLAPEGDF
                     SSLVNPLAARPGLTLRTVPLEALADEVRPGTALVAFSAVQSRDGRIADMAAVREAARAH
                     GARTYLDATQAAGWLPLRAADFDFVVAGAFKWLLCPRGTTFMAFGGELGAGAPWPAAPH
                     AGWVAGEDLGASNYGLIRPAATARRFDEPHAFYSYIGAEQSLALIDELGVDTIHAHNTA
                     LAAHYRAGLAERGLTPVSAPGSAIVSTPGLTDAEHRLAEAGVRISVRGGLLRAAFHLYN
                     STDDVERLLSLVG"
     aSDomain        1747..2742
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CPH93_RS26770_Aminotran_5.1"
                     /evalue="7.20E-23"
                     /label="CPH93_RS26770_Aminotran_5.1"
                     /locus_tag="CPH93_RS26770"
                     /protein_end="348"
                     /protein_start="16"
                     /score="73.0"
                     /tool="antismash"
                     /translation="TYLNSASSGLLPARSAAALRAALDESASYGTMGRDYFAAASASRE
                     AFARLMGVPAERVAVGSSVAVQSAFVASSLPADAEVLAPEGDFSSLVNPLAARPGLTLR
                     TVPLEALADEVRPGTALVAFSAVQSRDGRIADMAAVREAARAHGARTYLDATQAAGWLP
                     LRAADFDFVVAGAFKWLLCPRGTTFMAFGGELGAGAPWPAAPHAGWVAGEDLGASNYGL
                     IRPAATARRFDEPHAFYSYIGAEQSLALIDELGVDTIHAHNTALAAHYRAGLAERGLTP
                     VSAPGSAIVSTPGLTDAEHRLAEAGVRISVRGGLLRAAFHLYNSTDDVERL"
     gene            2858..3925
                     /locus_tag="CPH93_RS26775"
                     /old_locus_tag="SAMN06272775_5450"
     CDS             2858..3925
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092358.1"
                     /locus_tag="CPH93_RS26775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_5450"
                     /product="alkene reductase"
                     /protein_id="WP_096214297.1"
                     /transl_table=11
                     /translation="MTTAFDPMDLAGIRLAHRIVMAPMTRNRATAEGVPTPSMVRYYTQ
                     RASAGLIITEAAQTDPIGRGYPLTPGLHTPAQIAGWREVTDSVHAAGGRIFAQLWHGGR
                     IGHPVLLADDLLPVAPSAVAAAGQVFTHEGLKDFVTPRALTGEEIAETIAGFADAARNA
                     LEAGFDGVEIHGANGYLIHQFLAPGSNLRTDEWGGSDEARARFAVEVVKAVAGAVGAHR
                     TGLRLSPGNPFNDIHEPAPEATYTTLVDAVAPLGLAYLHTCDVRDTELLSRLRQRFGGT
                     FLLNPATDGRPTGPEELSLVERGEADLLAYGELFLANPDLPRRLARGGPFNTPDRESFY
                     GGDDSGYLDYPALDA"
     gene            complement(4011..4910)
                     /gene="thpD"
                     /locus_tag="CPH93_RS26780"
                     /old_locus_tag="SAMN06272775_5451"
     CDS             complement(4011..4910)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606339.1"
                     /locus_tag="CPH93_RS26780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_5451"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_093482576.1"
                     /sec_met_domain="PhyH (E-value: 5.2e-38, bitscore: 130.1,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGTAEVITPRKDPVVWSQPGTAGPFAPSELS
                     DFERDGFFAIGELLTAEEVAVYRAELDRLVLDPTMRADPRSIVEKKTQTVRSVFEVHKI
                     SEVFAKLVSDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTENYDTNGGLMIMPGSHQHFVGCEGATPKDNYKRSLQMQDAGIPSDEV
                     LTKMADKHGIRLFTGKAGSATLFDCNAMHGSGDNITPYPRSNVFIVFNSVENAAVEPFA
                     APVRRPEYIGARDFTPVR"
     gene            complement(5001..5417)
                     /locus_tag="CPH93_RS26785"
                     /old_locus_tag="SAMN06272775_5452"
     CDS             complement(5001..5417)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606340.1"
                     /locus_tag="CPH93_RS26785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_5452"
                     /product="ectoine synthase"
                     /protein_id="WP_006606340.1"
                     /sec_met_domain="ectoine_synt (E-value: 3e-53, bitscore:
                     177.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLQDIEGTDRHVKAKSGTWESKRIVLAKERVGFSLHETTLY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEKHTITPGTMYLLDGHEKHTMRIKKDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPDPEPETV"
     gene            complement(5473..6741)
                     /gene="ectB"
                     /locus_tag="CPH93_RS26790"
                     /old_locus_tag="SAMN06272775_5453"
     CDS             complement(5473..6741)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 1.7e-75.
                     Score: 246.1. Matches aSDomain:
                     nrpspksdomains_CPH93_RS26790_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="CPH93_RS26790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_5453"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_093482575.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4e-78, bitscore:
                     261.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRSWPTVFDRAQGSRMTDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLESFQNIILRPRD
                     LPYKVMFPGPTGANSVEAALKLARKVKGRESIVSFTNAFHGMSLGALAVTGNSMKRAGA
                     GIPLVHGTPMPFDNYLDGTYPDFLWFERLLEDQGSGLNTPAAVIVETVQGEGGINVARP
                     EWLRALSDLCERWDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPLA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTAAATLDTYWADGQMEKQTLARGEIVEAHLK
                     AIAEEHPDAIAEYRGRGLVWGLECNDKPLADKIAKRAFELGLLIETSGPESEVVKLLPA
                     LTTTPEELDEGLRILARAVRDCA"
     aSDomain        complement(5647..6657)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CPH93_RS26790_Aminotran_3.1"
                     /evalue="1.70E-75"
                     /label="CPH93_RS26790_Aminotran_3.1"
                     /locus_tag="CPH93_RS26790"
                     /protein_end="365"
                     /protein_start="28"
                     /score="246.1"
                     /tool="antismash"
                     /translation="FDRAQGSRMTDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMSTTAKRAFLESFQNIILRPRDLPYKVMFPGPTGANSVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGALAVTGNSMKRAGAGIPLVHGTPMPFDNYLDGTYPDFLWFER
                     LLEDQGSGLNTPAAVIVETVQGEGGINVARPEWLRALSDLCERWDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPLALTLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAATLDTYWADGQMEKQTLARGEIVEAHLKAIAEEHPDAIAEYRGRGLVWGLECN"
     gene            complement(6876..7442)
                     /gene="ectA"
                     /locus_tag="CPH93_RS26795"
                     /old_locus_tag="SAMN06272775_5454"
     CDS             complement(6876..7442)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156969.1"
                     /locus_tag="CPH93_RS26795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_5454"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_093482574.1"
                     /transl_table=11
                     /translation="MTAAQADHAGARSDFREFPEGFKLDTPRVEDGAAIWRIARDSKAL
                     DLNSSYSYLLWCRDFAATSVVARDAKGEPAAFVTGYIRPERPETLVVWQVAVDAAHRGQ
                     GLAAALLDGLTARATDALGVRFVETTITPDNAASNRLFASFAERHSVPIKREVLFDAGL
                     FPEQGHEPEVLHLIGPFPPPARSGH"
     misc_feature    7598..7613
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    7608..7623
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     gene            8041..9132
                     /locus_tag="CPH93_RS26800"
                     /old_locus_tag="SAMN06272775_5455"
     CDS             8041..9132
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960017.1"
                     /locus_tag="CPH93_RS26800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_5455"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_096215607.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.5e-16, bitscore:
                     59.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEGAVLHLKGRVLVGPDEVRDELWVVGGRVTFDRPTMARDATTV
                     TGWALPGLVDAHCHVGLDAHGPVDDATSEKQALTDRDAGTLLIRDAGSPSDTRWIDDRA
                     DLPRIIRAGRHIARTKRYIRNYAHEIEPADLVAYVAREARRGDGWVKLVGDWIDRSTGD
                     LEACWPPGAVEAAIAEAHRLGARVTAHCFAEDSLAPLVEAGIDCIEHATGLTEDTIPLF
                     AERGVAIVPTLVNIATFPLLADGGEAKFPRWADHMRRLHARRYDTVRAAYDAGVPVFAG
                     TDAGGSLAHGLVAEEVVELTRAGLPAVAALSATTWGARDWLGRPGLTEGASADLVVYES
                     DPREDVRVLAAPRRVVLRGEVVG"
     gene            9300..10121
                     /locus_tag="CPH93_RS26805"
                     /old_locus_tag="SAMN06272775_5456"
     CDS             9300..10121
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606344.1"
                     /locus_tag="CPH93_RS26805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_5456"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_096215608.1"
                     /transl_table=11
                     /translation="MHTDTSPHRTPPRHRRPAVLAAVALVTVSAAAALAGCNGGSGGGA
                     DADSSAQQQKAGKKVLWMGDSIAGAEAPPLEAALKASGQRFKDASSDGGGTVVASDTMA
                     GSIAQDTWKDLKKNLASFRPDVIAYQLTTYDWGTPAQQRAAYEKLAKTARDAGAELDLV
                     SAPPFKIDDFYKKYEGAIKSAPKVAKEVADKSGGTVHFLDATPLWGTDSAGKQAQRSPD
                     GIHSCQQGSAAFAKWFTEQLGKQDGFTPAAPEKWAKGSWTGSKRYVPLKCS"
ORIGIN
        1 gcgcgacctc tgtcgttgac aggctgcgcc ggtgaatcgt gaacagatct ccaggatcgc
       61 ccatgctgag cacccgataa agtctccgct cgacgacgac tcggtgcgtc ggctgctgga
      121 acgcgggctc ccacgcggcg acgagcgggt gctcgacctc ggttgtggcg gcgcggaatg
      181 gctcctgcgc gcgctggcca tgtgcccgca cctgcacgcc gtaggcgtgg acatttccga
      241 ggacgccctg gcacaggccc gccgggcggc gagcgacctc ggcgtgcaag agcgcctcgt
      301 tctccatcac caggaagccg gcgacttcgt gtcctcgcag ccgttcgacc tggtgctcag
      361 cttcggggcc acgcatgcct tcggtggtct gctccccacc ctcgcggcgg cacgcaggca
      421 cctggcaccc ggcggccgcg tcctcatcgg tgaaggcttc tgggaccgcg ccccttccca
      481 ggaggccaga gagatgctcg gggacttcac cgacctggcg accaccgtgg accgcgtcgt
      541 gtccgagggg tggacccccg tccacggaca cgtcagcaca cgccgtgagc tggacgacta
      601 cgaatgggcc tgctggggct cgctggcctc ctgggccctg gaccaccccg ccgatccggc
      661 cagctcgcac gtgctcacga cagccaccac ccgtcgctcc gaatggctgc gcgcctaccg
      721 ggacacctgg ggcttcgtct ccctggtcct gcgccggacg cccgactgcc cctgaacctc
      781 ctgtggcgag gtcatgccgg gacgccaccc ccgggaatag ccccgcaccc cgaaaggttg
      841 caccagggca cacccacgac gaggaaagtc agccatccca tgcgcgtcga aatctggtcc
      901 gacatcgcct gcccctggtg ctatatcggc aaggcccgct tcgaggccgg gctcgccgcg
      961 ttcgcgcacc gtgaggtcgt cgaggtggtg caccgctcct tcgagctgga cccgaatgcg
     1021 ccggccgccg acggcgtccc gatgctcgac atgctcgccg cgaagtacgg cgtgacccgc
     1081 gagcaggccg cggcgatgga ggcccgggtc gccgaggccg cggctgggga ggggctggac
     1141 taccgctccg accggatcca cggcaacacc ttcgacctgc accggctgct gcacctgggc
     1201 aagttgtacg gcgtccagga cgccctgctc gacgccttct accgcgccaa cttcgctgag
     1261 gcccgggcgc tcggcgaccc cgccgtccgc agcgagatcg ccgtcggcgc ggggctgccc
     1321 gccgacgagg tcgcccgggt gctggccgac cccggggcgt atgcccagga ggtgcgcgcc
     1381 gaggagcgcg cggcggcgga gctgggcgcc accggcgtgc cgttcttcgt catcgaccgc
     1441 cgctacggca tctccggcgg acagcccgcc gaggtcttcc ggcaggccct ggagcgggcg
     1501 tacgccgacg gcggcgagcc gctgtcgacc gccggcgagg gcgaggcgca tggggacgac
     1561 ggctgcgccg tgcccgggac atgaccgcgc cgccggcctg ccgcacccgc gcggggccgt
     1621 aaggcccggt ggcgggatac ggcccgcgtc acggcccgtg ccatggactg gccgacgatc
     1681 acccaccaca gtgggcacat gcaacgacgt gagccactcg gcggggccga attcgcgccc
     1741 gagacgacgt atctgaactc tgcctccagc ggactcctcc cggcgcgcag cgccgccgcg
     1801 ctgcgtgctg cgctcgatga atccgcctcc tacggcacga tgggccgcga ctacttcgcc
     1861 gccgcctcgg cgtcccggga ggccttcgcc cggctgatgg gcgtgcccgc cgagcgggtc
     1921 gccgtcggca gttcggtcgc ggtgcagtcc gcgttcgtgg cctcctcgct tccggcggac
     1981 gccgaagtgc tggcccccga gggcgacttc agctccctgg tcaatccgct cgccgcccga
     2041 ccgggcctca cgctgcgcac cgtaccgctg gaggcgctcg ccgacgaggt gcgccccggc
     2101 accgccctgg tcgccttcag cgccgtgcag tcgagggacg gccggatcgc cgacatggcg
     2161 gcggtgcgtg aggccgcgcg ggcgcacggg gcgcgcacct acctcgacgc cacccaagcc
     2221 gccggctggc tgccgctgcg cgccgcggac ttcgacttcg tcgtcgccgg cgccttcaag
     2281 tggctgctgt gcccgcgcgg cacgaccttc atggccttcg gcggggagct gggcgcgggc
     2341 gcgccgtggc cggccgcgcc gcatgcgggc tgggtcgccg gtgaggacct cggtgcgtcc
     2401 aactacggcc tgatccggcc ggccgccacc gcccggcgct tcgacgagcc gcatgccttc
     2461 tactcctaca tcggcgcgga gcagtcgctc gccctcatcg atgagctggg ggtggacacc
     2521 atccacgccc acaacaccgc actcgccgcc cactaccgtg ccgggctggc cgagcgcggc
     2581 ctgacgccgg tttccgcgcc gggctcggcg atcgtctcca cgccgggtct cacggacgcc
     2641 gaacaccggc tggccgaggc gggggtacgg atctcggtgc gcggcggact gctccgcgcg
     2701 gccttccatc tgtacaactc gacggatgat gtggagcggc tgttgtccct ggtggggtag
     2761 ggccgggccg agccggggaa tgcccgcggg gcccccggtg tcgagactga tgtaatgggc
     2821 aacggacatg ttccgagcag cagttgagag gccctcagtg accaccgcat tcgaccccat
     2881 ggacctcgcg ggcatccggc tcgcccaccg catcgtcatg gcgccgatga cccgcaaccg
     2941 cgccaccgcc gagggcgtcc ccaccccgtc catggtccgc tactacaccc aacgggcctc
     3001 ggccggcctc atcatcaccg aggcggccca gaccgacccc atcggccgcg gctacccgct
     3061 cacccccggt ctgcacaccc ccgcacagat cgccggatgg cgcgaggtca ccgactccgt
     3121 gcacgccgcg ggcggcagga tcttcgcgca gctgtggcac ggcggccgca tcggccaccc
     3181 ggtgctgctg gccgacgacc tgctgccggt ggcgccctcc gcggtcgccg cggcgggcca
     3241 ggtcttcacc cacgagggcc tcaaggactt cgtcaccccg cgcgcgctga ccggcgagga
     3301 gatcgccgag accatcgccg ggttcgcgga cgccgcccgc aacgccttgg aggccgggtt
     3361 cgacggggtg gagatccacg gcgccaacgg ctatctgatc caccagttcc tggcccccgg
     3421 ctccaacctg cgcaccgacg agtggggcgg ctccgacgag gcccgggccc gtttcgccgt
     3481 cgaggtcgtc aaggccgtcg ccggcgcggt cggcgcgcac cgcaccggtc tgcggctctc
     3541 gcccggcaac cccttcaacg acatccacga gcccgcgccg gaggccacgt acaccacgct
     3601 ggtcgacgcc gtcgccccgc tgggcctggc ctacctgcac acctgcgacg tccgtgacac
     3661 ggagctgctg agccggctgc gccagcgctt cggcgggacg ttcctgctca acccggccac
     3721 cgacggccgg cccaccgggc ccgaggagct gtcgctcgtc gagcggggcg aggccgatct
     3781 cctcgcgtac ggcgagctgt tcctcgccaa ccccgacctg ccccgccgcc tggcccgcgg
     3841 cggccccttc aacacccccg accgggagag cttctacggc ggcgacgact ccggctacct
     3901 cgactacccg gcactggacg cctgagcagc ggccggcccg cgcaacgcac acggcgcggc
     3961 cgccgcacag accctgctgt gcggcggccg cgccaccggg atctccgcga ctacctcacc
     4021 ggcgtgaagt cacgggcccc gatgtactcc gggcgccgga ccggcgccgc gaacggctcc
     4081 accgccgcgt tctccacgct gttgaagacg atgaagacat tgctgcgcgg gtacggcgtg
     4141 atgttgtcac ccgagccgtg catggcgttg cagtcgaaca aggtcgccga gccggccttg
     4201 cccgtgaaga ggcggatgcc gtgcttgtcc gccatcttcg tcagcacctc gtccgacggg
     4261 atgcccgcgt cctgcatctg cagcgaccgc ttgtagttgt ccttcggcgt cgcaccctcg
     4321 cagcccacga agtgctggtg cgaaccgggc atgatcatca agccgccgtt ggtgtcgtaa
     4381 ttctccgtca gcgcgatcga gaccgacacg gtgcgcatgt tcggcagccc gtcctcggcg
     4441 tgccaggtct cgaagtccga gtgccagtag aagccggagg caccgaagcc cggcttcaca
     4501 ttgatccgcg actggtggac gtagacgtcc gagccgagga tctgccgggc acggccgacc
     4561 acgcgcgggt cggagaccag cttggcgaac acctcgctga tcttgtgcac ctcgaacacg
     4621 gaccgcacgg tctgcgtctt cttctcgacg atggagcgcg ggtcggcgcg catcgtcggg
     4681 tcgagaacga gccggtccag ttcggcgcgg tacaccgcga cttcttccgc cgtcagcagc
     4741 tccccgatgg caaagaagcc gtcgcgttcg aagtcgctca gctcggacgg cgcgaacggg
     4801 ccggccgttc cgggctgcga ccacaccacc gggtccttcc gcggggtgat gacctcagcg
     4861 gtcccacggg tcgggtacag gtcggcggtg cgctcgggtg cggtggtcat ggtgttgcct
     4921 tcctctcctc tcgtacgggc tctggcttac gtggtacggg ccttcctcag ctgccggacg
     4981 gggcaggcgg ggggtgcgcc tcagaccgtt tcgggctccg gatccggctc ggtgagcagc
     5041 gggtagacac cgttctcgtc gtggtcctca cggccggtga ccggcgggtt gaagacgcag
     5101 acgcagcgga agtccttctt gatccgcatg gtgtgcttct cgtgtccgtc gagcaggtac
     5161 atcgtgcccg gggtgatggt gtgcttctcg ccggtctcgt cgttggtgag ttcggcctct
     5221 ccctctacgc agagtacggc ctcgatgtgg ttcgcgtacc acatcgacgt ctccgtaccg
     5281 gcgtacaggg tggtctcgtg cagggagaag ccgacgcgct ccttggcgag cacgatgcgc
     5341 ttgctctccc acgtgccgga cttggccttg acgtggcggt cggtgccttc gatgtcctgg
     5401 agggatcgga cgatcacggt gcggggtgcc tttctgtgga acggtaagga acgctggggg
     5461 ccgttgggcc gatcaggcgc agtcgcggac cgcgcgggcg aggatgcgca gaccctcgtc
     5521 cagttcctcg ggggtggtcg tcagggccgg aagcagcttg acgacctcgc tctccgggcc
     5581 ggaggtctcg atcagcagac ccagctcgaa ggcgcgcttg gcgattttgt cggccagggg
     5641 cttgtcgttg cactccaggc cccacaccag accgcggccg cggtactcgg cgatggcgtc
     5701 cgggtgctcc tcggcgatgg ccttcaggtg tgcttcgacg atctcaccgc gggcgagagt
     5761 ctgcttctcc atctggccgt cggcccagta ggtgtccagc gtcgcggcgg cggtgacgaa
     5821 ggccgggttg ttgccgcgga aggtgccgtt gtgctcgccc ggctcccaga tgtccagctc
     5881 gggcttgaac agggtgagcg cgagcggcag gccgtagccg ctgatggact tcgagacggt
     5941 gacgatgtcc ggcacgatgc ccgcctcctc gaaggagaag aaggcacccg tacggccgca
     6001 gcccatctgg atgtcgtcga cgatgagcag catgtcccag cgctcacaca ggtcggacag
     6061 ggcgcgcagc cactcgggcc gggccacgtt gatgccgccc tcgccctgga ccgtctcgac
     6121 gatcacggcg gcgggggtgt tcagaccgga gccctggtcc tccagcagcc gctcgaacca
     6181 caggaagtcc gggtaggtgc cgtcgaggta gttgtcgaac ggcatcgggg tgccgtgcac
     6241 cagcgggata ccggcgccgg cccgcttcat ggagttaccg gtgacggcga gcgcgcccag
     6301 cgacatgccg tggaaggcgt tggtgaagga cacgatcgac tcgcggccct tgaccttacg
     6361 ggccagcttc agcgcggcct cgacggagtt ggcgcccgtc gggcccggga acatgacctt
     6421 gtagggcagg tcgcgcggcc gcaggatgat gttctggaac gactccagga aggcccgctt
     6481 ggccgtggtg gacatgtcca ggccgtgggt gaccccgtcc cgctcgatgt agtcgatcag
     6541 agcgcgtttg agtacggggt tgttgtggcc gtagttgagc gagccggcgc cggcgaagaa
     6601 gtcgaggtag gtgtgaccgt cctcgtcggt catgcggctg ccctgcgcac ggtcgaagac
     6661 ggtgggccag ctacggcagt agctgcggac ctccgactcc acggtctcga agacgctcag
     6721 atcgggctgg gtgatggtca cagcatgctc ctgggagatg agtggcgaaa gatgggaagt
     6781 ctgtgggggc tgggcggtgc cggcgggtgt cgcggggtgt gtgccggatc cgcatggccg
     6841 ctgcggcgcg gtggccgcgc ggccctcggg ctcggtcagt gccctgagcg ggcgggtggg
     6901 gggaacgggc cgatgaggtg cagcacctcc ggctcgtggc cctgctcggg gaacagccct
     6961 gcgtcgaaga gcacctcgcg cttgatcggc accgagtgcc gctcggcgaa ggacgcgaac
     7021 aaccggttcg atgcggcgtt gtcgggggtg atggtggtct cgacgaaacg cacgcccagc
     7081 gcgtccgtgg cacgggcggt gagcccgtcc aggagtgcgg ccgccagccc ctggccacgg
     7141 tgcgcagcgt cgacggccac ctgccagacg acgagcgtct ccgggcgctc ggggcggatg
     7201 tatccggtga cgaaggcggc cggctcaccc ttcgcgtcgc gagcgacgac ggaggtggcg
     7261 gcgaagtcgc gacaccacag gaggtagctg taggaggagt tgaggtccag cgccttggag
     7321 tcgcgggcga tgcgccagat cgcggctccg tcctccactc gtggggtgtc gagcttgaag
     7381 ccctccggaa attctctgaa atcgcttcgg gcacctgcat ggtctgcttg tgcggcggtc
     7441 atgggaatta aatttaccca gggaaaaagg aaattgcatc gcgggcaggg gttacggaag
     7501 cgacgaagat gtgttatcgc gcgggggcgc ccgtccgcgc gatgccaccg caaaatgtca
     7561 cgatttgacc aggatgttcg gggcgaaacg gacacgttgt gtagtcggtc acagctccgt
     7621 aacgcccagg agatgcgtcc gaattacggt acttgccact agcaaaatct tggcgtttga
     7681 ggtgcggaaa agcgggcaga agaatacggg aagctgtacc gcaagggaat tgggaattcg
     7741 cctcccgaag gctcggcgta agcccatcga aacctgagcg acaagtccgt gacaagaccc
     7801 cggacaccat gcgcgagatg atgttggccg acccgtgaga acggacacgg aacgtgtgcg
     7861 gaacgctccc ggaagcggac acggaacgta tgccgaccgc acacggagag agcgcagcca
     7921 aaagcacggg caatgcgccg ggttcccggg gaagcggccg cgcgaaagcg cccgccggcc
     7981 cgttgcggca gcagcgtccg cccaccgcac ctcggcgcct aaagtgccgg tatgacgtcg
     8041 atgagtgagg gtgcggtgct gcacctcaag gggcgggtac tcgtcgggcc ggacgaggtc
     8101 cgcgacgagc tgtgggtcgt cggtggccgg gtcaccttcg accggccgac gatggcacgg
     8161 gatgccacca ccgtgacggg ctgggcgctg ccgggcctgg tcgacgccca ctgccatgtg
     8221 ggcctggacg cgcacggccc ggtggacgac gccaccagcg agaaacaggc gctcaccgac
     8281 cgtgacgcgg ggaccctgct gatccgcgac gccggctcgc cctccgacac ccgctggatc
     8341 gacgaccgcg cggacctgcc acggatcatc cgcgccggcc gtcacatcgc ccgtaccaag
     8401 cgctacatcc gcaactacgc ccacgagatc gagcccgcgg acctggtcgc gtacgtcgcc
     8461 cgggaggccc gtcgcggcga cggctgggtc aagctcgtcg gcgactggat cgaccgctcc
     8521 acgggcgatc tggaggcctg ctggcccccg ggcgcggtcg aggcggccat cgccgaggcg
     8581 caccggctcg gcgcccgggt gaccgcccac tgcttcgcgg aggactccct cgccccgctc
     8641 gtcgaggccg gcatcgactg catcgagcac gccaccggtc tcaccgagga caccatcccg
     8701 ctgttcgccg agcgcggcgt cgccatcgtc cccacgctcg tcaacatcgc cacgttcccg
     8761 ctcctcgcgg acggcggcga ggcgaagttc ccgcgctggg cggaccacat gcggcggctg
     8821 cacgcgcgcc gctacgacac cgtacgggcg gcctatgacg ccggggtgcc cgtcttcgcc
     8881 ggtacggacg ccggcggctc gctcgcccac gggctggtcg ccgaggaggt cgtcgagctg
     8941 acccgggccg ggctgcccgc cgtggcggcc ctctccgcca ccacctgggg cgcccgggac
     9001 tggctgggcc gccccggcct caccgagggt gcctcggcgg acctggtggt ctacgagagc
     9061 gatccgcgcg aggacgtccg ggtgctggcc gcgccccggc gggtggtgct gcgcggggag
     9121 gtcgtgggct agggcggcct cgccccacgg ccggggggca cccctggtag ccgggcggcc
     9181 ccgctccgcc acagcggccg ggcgaggccg tgtggtgttc catgacggcc tgatccccgt
     9241 cctgtgagca accgatgacg accaggctga ccggcgattt ccgctggttt ggtggggaca
     9301 tgcacaccga taccagtcct catcgcacgc ccccacgtca ccggcgcccg gcggtcctcg
     9361 ccgctgttgc cctggtcacc gtgagtgccg ctgccgccct ggccggctgc aacggcggat
     9421 ccggcggcgg ggcggacgcg gactcctccg cgcagcagca gaaggccggc aagaaggtcc
     9481 tgtggatggg ggactccatc gccggcgccg aggccccgcc cctggaggcg gcgcttaagg
     9541 cgagcggcca gcgcttcaag gacgcctcgt ccgacggcgg tggcacggtc gtcgcgagcg
     9601 acaccatggc cggctcgatc gcacaggaca catggaagga cctgaagaag aacctcgcgt
     9661 cgttccggcc ggacgtgatc gcctaccagc tcaccaccta cgactggggc acgcccgcgc
     9721 agcagcgtgc cgcgtacgag aagctcgcga agaccgcgcg ggacgccggc gccgagctcg
     9781 acctcgtctc cgcgccgccg ttcaagatcg acgacttcta caagaagtac gagggtgcga
     9841 tcaagagcgc gccgaaggtg gccaaggagg tcgccgacaa gagcggcggc acggtgcatt
     9901 tcctcgacgc caccccgctg tggggcaccg acagcgccgg taagcaggcc cagcgctccc
     9961 cggacggcat ccactcctgt cagcagggct cggccgcctt cgccaagtgg ttcaccgagc
    10021 agctcggcaa gcaggacggc ttcaccccgg ccgcgccgga gaagtgggcg aagggctcat
    10081 ggaccggcag caagcggtac gtgccgctca agtgcagctg accccccgtg gccccgcgcc
    10141 ggctccggcg caccgtctcc ttcccccttc gtacgtggcg gaccggccga tgcgcccgca
    10201 ccggccggtc cgccgaggac tccgccatgg ctgacaccct tcccgtaccg tccgtgcacc
    10261 gccgtccccg ccccggccgc gctcccggcc gtgcccatat cgccccgctc gacgggctgc
    10321 gcggcctggc cgtcgccgcc gtgctgctgt tccacgccgg ctacctgggc ggcggcttcc
    10381 tcggtgtcga cctgttcttc gtcctgtcgg gcttcct
//
