LOCUS       NZ_LT907981            10978 bp    DNA     circular CON 12-FEB-2024
DEFINITION  Streptomyces sp. 2323.1 chromosome I.
ACCESSION   NZ_LT907981
VERSION     NZ_LT907981.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. 2323.1
  ORGANISM  Streptomyces sp. 2323.1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2017) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 03:31:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,058
            CDSs (total)                      :: 6,971
            Genes (coding)                    :: 6,808
            CDSs (with protein)               :: 6,808
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 64 of 163
            Pseudo Genes (incomplete)         :: 123 of 163
            Pseudo Genes (internal stop)      :: 17 of 163
            Pseudo Genes (multiple problems)  :: 37 of 163
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:07:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1880307
            Orig. end                         :: 1891285
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT907981.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10978
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="14"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10978
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10978
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5978](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5978
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(19..34)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 20.97"
                     /tool="antismash"
     gene            241..1092
                     /locus_tag="CPH93_RS07485"
                     /old_locus_tag="SAMN06272775_1556"
     CDS             241..1092
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602520.1"
                     /locus_tag="CPH93_RS07485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_1556"
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="WP_093485853.1"
                     /transl_table=11
                     /translation="MNDQQTQRTPRSLIVTFYGAYGRGEPGTAGGPAAPVPVAALIRLL
                     GALGVDPPSVRSAVSRLKRRGLLVPARTATGAAAYGLSDDARQLLEDGDRRIFGRPAAQ
                     LSDGWVLAVFSVPEEERHKRHLLRSRLARLGFGTAAPGVWIAPAQLYEETRHTLQRLQL
                     DPYVDLFTGAHTGFAPTADAVARWWDLDAVAAAHRAFLAEQEPVLRRWSPRRSVPPEAA
                     YRDYLLALDAWRQLPYADPGLPSPLLPEDWPGGRAAEVFGLLHAKLHTAGARYAHELTT
                     GA"
     misc_feature    278..292
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.44"
                     /tool="antismash"
     gene            complement(1110..1874)
                     /locus_tag="CPH93_RS07490"
                     /old_locus_tag="SAMN06272775_1557"
     CDS             complement(1110..1874)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602521.1"
                     /locus_tag="CPH93_RS07490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_1557"
                     /product="HAD family hydrolase"
                     /protein_id="WP_093649203.1"
                     /sec_met_domain="HAD_2 (E-value: 6.2e-28, bitscore: 96.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVATEVGERPVRGARGAGGETIRAAVFDLDGTLANTLPAISKLL
                     VKVASEQGCSVTPRQAAAAIGKPPGPAFGRLLGRPEDDGRVQAAISRYRELFSYEVLCQ
                     GPQLLFPGVTAGLSALRAHGVELAVATSKTTRSAESLLDVMGIRDLVGPVIGQDMVRRG
                     KPHPEMVLRAAGRLGVAAWQSAYVGDTVGDMRMAVTARMEPVAVTYGVGKFGELAGVAG
                     TRMCSSFKDVVSVIAAARPRSGVTVGAQQRRR"
     misc_feature    complement(2065..2079)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     gene            2328..2963
                     /locus_tag="CPH93_RS07495"
                     /old_locus_tag="SAMN06272775_1558"
     CDS             2328..2963
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602522.1"
                     /locus_tag="CPH93_RS07495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_1558"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_093485849.1"
                     /transl_table=11
                     /translation="MPTQHRAIQSRQALIRSAAETFLEKGVPAAGMVEISRRARLSKGA
                     LYFHFTSKDDLTLAVRDAALATLQEVEEAFTRSPQPLTTAVQDFTVELFGRVRTDAVLR
                     AGLRLRPETAPGLGIYALEQRWYALFLDKAVTCGPCGPAGSHAPDAEPRRTAQLLTSLV
                     VGLLHLGGEDGTWWDEESIVGMWGLLPAAPSRIAAAGIPAPAQLPAAG"
     gene            complement(3016..4044)
                     /locus_tag="CPH93_RS07500"
                     /old_locus_tag="SAMN06272775_1559"
     CDS             complement(3016..4044)
                     /NRPS_PKS="Domain: PKS_ER (81-340). E-value: 3.2e-20.
                     Score: 64.4. Matches aSDomain:
                     nrpspksdomains_CPH93_RS07500_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088829.1"
                     /locus_tag="CPH93_RS07500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_1559"
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_093485847.1"
                     /sec_met_domain="ADH_zinc_N (E-value: 8e-18, bitscore:
                     63.4, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPTVVPAIPAVHREVRLADQAEGELTAGHFTVVEVPVPVPEPGQ
                     VLVRTRVMAVTAAMRTQMTKVPLPMPSFVPGQALWGAAVGEVVAAPGGGFAPGELVHHP
                     YGWREFAAVEESRVRRLDPDALPTPAAHLSQGATAWGALRRAAEVRPGDTVFVTGAAGG
                     VGSLAGQLARRLGAGRVVGSTGSERKAARLRAELGYDDTIVRGAGPIERQLHRAAPGGI
                     DVLLDTVGGEQLPAALAVARPDARFALVGALSSQLGGSGGALTPVELDTGLIITRRVTL
                     RGFGLYAHPDLPQEWTKEFGQGLREGSLTFPHALLQGIEQAPRALCELTQGRHIGAVLV
                     EL"
     aSDomain        complement(3025..3801)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CPH93_RS07500_PKS_ER.1"
                     /evalue="3.20E-20"
                     /label="CPH93_RS07500_PKS_ER.1"
                     /locus_tag="CPH93_RS07500"
                     /protein_end="340"
                     /protein_start="81"
                     /score="64.4"
                     /tool="antismash"
                     /translation="AAVGEVVAAPGGGFAPGELVHHPYGWREFAAVEESRVRRLDPDAL
                     PTPAAHLSQGATAWGALRRAAEVRPGDTVFVTGAAGGVGSLAGQLARRLGAGRVVGSTG
                     SERKAARLRAELGYDDTIVRGAGPIERQLHRAAPGGIDVLLDTVGGEQLPAALAVARPD
                     ARFALVGALSSQLGGSGGALTPVELDTGLIITRRVTLRGFGLYAHPDLPQEWTKEFGQG
                     LREGSLTFPHALLQGIEQAPRALCELTQGRHIGAVLV"
     CDS_motif       complement(3529..3591)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CPH93_RS07500_0001"
                     /evalue="1.50E-03"
                     /label="PKSI-ER_m5"
                     /locus_tag="CPH93_RS07500"
                     /protein_end="172"
                     /protein_start="151"
                     /score="11.3"
                     /tool="antismash"
                     /translation="GDTVFVTGAAGGVGSLAGQLA"
     gene            4238..4855
                     /locus_tag="CPH93_RS07505"
                     /old_locus_tag="SAMN06272775_1560"
     CDS             4238..4855
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981105.1"
                     /locus_tag="CPH93_RS07505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_1560"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_093649201.1"
                     /transl_table=11
                     /translation="MVKQERAVRTRRAVLEAAAHVIGTRGYQAATMAEIIQRAGVTKGA
                     VYFHFTSKDALARAVITEQTDPFLPQVSESRLQDAIDFTHQVALALRSDPLLQAGTRIA
                     VETTFSENPLVPYQAWTDIITTMFTEARDNGELLPGVAPDRAAEFFVAAYMGVQLFSRA
                     ATNRADLPERVTALWKHTLPGLASPGALSHLDPHGRCVKVPA"
     misc_feature    4646..4648
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5001..5978
                     /locus_tag="CPH93_RS07510"
                     /old_locus_tag="SAMN06272775_1561"
     CDS             5001..5978
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602526.1"
                     /locus_tag="CPH93_RS07510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_1561"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_093485845.1"
                     /sec_met_domain="AfsA (E-value: 4.8e-23, bitscore: 79.7,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASATLTPYLRDERHDNGGEDANGIRPSALTTTVPREYVHRTAVS
                     EVFLTNWRQGDADSWVVSAQWPRAHTFYGPVHGLHDPLLLIESMRQAGILLSHVAHGVP
                     LDHPIIWQRVRYDLLPEALRTTDRPAEVELHITDHDMVHRGKRLASARQEFRIVCDGAE
                     LATAALDYSCHSPAVYRRLRGEYCDLQLANSRKLPVPEAVPPQLVARDHDRDVALSPTD
                     RPDRWQLRVDTSHPVLFDHPVDHTPGMLMIEAARQAAQATAPGFTLPVSMECSFARYAE
                     LDAPSWVQARTTDRADDGSRQLTVDVEQHDKPVVSAQITSVPMS"
     misc_feature    5283..5285
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    5355..5357
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6096..8417)
                     /locus_tag="CPH93_RS07515"
                     /old_locus_tag="SAMN06272775_1562"
     CDS             complement(6096..8417)
                     /GO_function="GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602527.1"
                     /locus_tag="CPH93_RS07515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_1562"
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="WP_096212359.1"
                     /transl_table=11
                     /translation="MRVAVIGGGPGGLYAAALLKRLDPTREITVWERNAPEDTFGFGVV
                     LSDETLGGIEHADPEVYRALQAEFVRWDDIDIVHRGRTLTSGGHGFAALGRRRLLAVLH
                     QRCRDLGIELRFRTEAPPAAELARTYDLVIAADGVHSATRTAHADIFGPQLTTHRCRYI
                     WLAADFALDAFRFEIAQTEHGVAQLHAYPYARPSSDHLATSPSGEHPDGASTVIVEMRE
                     EVWRAAGLDRCDERESAQWCAKVFADALRGRSLRANNSSWIAFRTVVNARWSHGNTVLL
                     GDAAHTAHFSIGSGTKLAVEDALALAACLQEQPDTASALAAYEEERRPVVASTQRAARA
                     SLAWFEELATYLDQPPHQFAFNLLTRSRRVTHDNLRLRDPGFVTAVEREAGIESATPPM
                     FTPFRLGGLTLRNRVVVSPMDMYSAVDGAGTPGDFHLVHLGARALGGAGLVMTEMVCVS
                     PEGRITPGGTGLYAPGHETAWRRVTDFVHTSAPGTAIGVQLGHSGRKGSTRLMWDGIDQ
                     PLPEGNWPLVAASPLPYRAGVNQLPHALTEPELATVREQFVRSAESAARAGFDLLELHC
                     AHGYLLSGFLSPLTNRRTDAYGGDVHARLRFPLEVFDAVRAVWPAGRPMTVRISATDWA
                     DGGTTARDAVAIAEAFARHGADAIDVSTGQVVPDERPDFGRSYQTPFADRIRNTLGVPV
                     IAVGAISSWDDVNSLVLAGRADLCALARPHLYDPHWTLHAAAEQGYAGPGAPWPLPYQA
                     GNRPPPTGRTDAPKPRLTLR"
     gene            complement(8420..9247)
                     /locus_tag="CPH93_RS07520"
                     /old_locus_tag="SAMN06272775_1563"
     CDS             complement(8420..9247)
                     /NRPS_PKS="Domain: ECH (18-267). E-value: 3.3e-51. Score:
                     165.9. Matches aSDomain:
                     nrpspksdomains_CPH93_RS07520_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982203.1"
                     /locus_tag="CPH93_RS07520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_1563"
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="WP_006602528.1"
                     /transl_table=11
                     /translation="MSPFAGSARSTEPWQHIRVTRKDGIATVTLARPDKLNALTFEAYA
                     DLRDLLAELSRERSVRALVLAGEGRGFCSGGDVEEIIGATLGMDTAQLLDFNRMTGQVV
                     RALRECPFPVIAAVHGVAAGAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYL
                     LPRVIGLGHATRLLMLGDPVRAPEAERLGLISELAEEGRADEAAAALAKRLAEGPALAH
                     AQTKALLTAELDMPLAAAVEMDAATQALLMNSDDYAEFHAAFTEKRTPKWQGK"
     aSDomain        complement(8447..9193)
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CPH93_RS07520_ECH.1"
                     /evalue="3.30E-51"
                     /label="CPH93_RS07520_ECH.1"
                     /locus_tag="CPH93_RS07520"
                     /protein_end="267"
                     /protein_start="18"
                     /score="165.9"
                     /tool="antismash"
                     /translation="VTRKDGIATVTLARPDKLNALTFEAYADLRDLLAELSRERSVRAL
                     VLAGEGRGFCSGGDVEEIIGATLGMDTAQLLDFNRMTGQVVRALRECPFPVIAAVHGVA
                     AGAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYLLPRVIGLGHATRLLMLGD
                     PVRAPEAERLGLISELAEEGRADEAAAALAKRLAEGPALAHAQTKALLTAELDMPLAAA
                     VEMDAATQALLMNSDDYAEFHAAFTEK"
     gene            9548..>9799
                     /locus_tag="CPH93_RS36695"
                     /pseudo=""
     CDS             9548..>9799
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714473.1"
                     /locus_tag="CPH93_RS36695"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLEPATPTWHIALPRSTTAVPIARAMIRAALQDLPVAADCPTAQL
                     LTSELVSNALQHTCGTEPIELVVEVQPTGCLIEVHDDDP"
     gene            <10352..10468
                     /locus_tag="CPH93_RS07525"
                     /pseudo=""
     CDS             <10352..10468
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017242483.1"
                     /locus_tag="CPH93_RS07525"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="HRRGLLLLRSLSSDAGCRRTPHGKAVWFTLPEVPWRRR"
     misc_feature    10953..10964
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
ORIGIN
        1 gcccggtctc cccgctcgtt ttcgcttccg tcgtcatgaa catcaaccta tctgggaggt
       61 ctgacagttg agagttctga tccaatccgg tctgcctcac caccgcggtg gtgggacacc
      121 gaaccgccct cccgacaatt cggccacccc ggccccgccc cgcaggagtc cgattctccc
      181 ccgcaaacgg gaggtacccc caccccggcc tgaaagccgg ggcatccccg gagtgacccg
      241 gtgaacgacc agcaaacgca gcgcacccca cggtccctga tcgtcacgtt ctacggcgcc
      301 tacggacgcg gcgagcccgg caccgcgggc ggcccggccg cgccggtgcc ggtcgccgcc
      361 ctgatccggc tgctcggcgc gctcggcgtg gatccgccgt cggtgcgctc cgcggtctcc
      421 cggctcaagc ggcgcggtct gctggtcccc gcgcgcacgg ccaccggggc ggcggcctac
      481 ggactgtccg acgacgcccg tcagctcctg gaggacggcg accggcggat cttcggccgg
      541 cccgccgccc agctgtccga cggctgggta ctggccgtct tctccgtccc cgaggaggag
      601 cggcacaaac ggcatctgct gcgctcccgc ctggcgcggc tcggtttcgg caccgccgca
      661 ccaggggtgt ggatcgcccc cgcacagctc tacgaggaga cccggcacac cctgcagcgg
      721 ctgcaactgg acccgtacgt cgatctgttc accggcgcgc acaccgggtt cgcccccacg
      781 gcggacgcgg tggcgcggtg gtgggacctc gatgcggtgg ccgcggcgca ccgcgccttc
      841 ctcgccgagc aggaacccgt cctgcgccgc tggtcaccac gccgctcggt gccccccgag
      901 gcggcctacc gcgactacct gctggcgctg gacgcctggc gtcagctgcc ctatgcggat
      961 cccggactgc cctctcccct cctgcctgag gactggccgg gcggccgcgc ggcggaggtg
     1021 ttcggcctgc tgcacgccaa actgcacacc gccggggccc gttacgcaca cgagctgaca
     1081 accggagcgt agcgccccct cccccggcct cagcgtcggc gctgctgggc ccccaccgtg
     1141 acgcccgacc gcggcctggc ggcggcgatc acggagacca cgtccttgaa ggagctgcac
     1201 atccgggtgc cggcgacccc ggcgagctcg ccgaacttcc cgacgccgta ggtcaccgcg
     1261 accggctcca tgcgcgcggt gaccgccatc ctcatgtccc ccacggtgtc ccccacgtac
     1321 gcgctctgcc aggccgcgac gccgagccgg ccggcggccc gcagcaccat ctccgggtgc
     1381 ggtttgccgc gccggaccat gtcctggccg atgaccgggc cgaccagatc acggatgccc
     1441 atgacatcga ggagcgactc ggcgctgcgg gtcgtcttgg acgtggcgac ggcgagctcg
     1501 acgccgtggg cgcgcagcgc ggacagcccc gcggtcacgc ccgggaacag cagctgcggc
     1561 ccctggcaca ggacctcgta ggagaacagc tcgcggtagc gggagatggc ggcctgtacc
     1621 cggccgtcgt cctcgggcct gcccagcagc cggccgaagg cggggccggg cggcttgccg
     1681 atggcggcgg ccgcctgccg cggggtcacc gaacagccct gctccgaggc caccttgacg
     1741 agaagtttgc tgatcgcggg gagagtgttg gcgagggtgc cgtccagatc gaagacggcg
     1801 gccctgatgg tttcaccgcc ggcgccccgc gcgcctctga cgggacgttc gccgacttcc
     1861 gttgctacgc gcatggctga gacctttccg gattcctatg ggggtgcagg gggctcgggt
     1921 gacgttgctg gctcggctgc tcgggcggcc tgcggtcgac gactggtgaa ggcagaggcg
     1981 gtctcgccat gacgcaccac ttccaagggc ggtacgcgac cgtcgcgggc ggctgcaacg
     2041 gaagggatcg ccccgtttac cgccttcgga gatttaaaaa ccgcctgcgc tgtttgtcaa
     2101 tgcatgcttg ctgcctctga cgggtcgaca acgctccgct tcccggccag actgggaacg
     2161 caccgcaccc cccgagcccc acaagatccg aacggccgac cgaacgtcag cttcgcgtca
     2221 gacagcgcca gttccggcca atcctgggga gcataccgaa ccgcatggtt ggttttctat
     2281 gtagactgca ttgagctcgc agccgtacta gagacgggac ggacaccgtg cccacccagc
     2341 atcgcgccat ccagagccgc caggcactca tccgctcggc cgcagagacc ttcctggaga
     2401 agggggtacc cgccgcgggc atggtcgaga tcagccggcg ggcccggctg agcaaaggtg
     2461 ccctctactt ccacttcacg tccaaggacg acctcacgct cgcggtgcgg gacgccgcac
     2521 tcgccaccct gcaggaggtc gaagaggcct tcacccgctc cccgcagccg ctgaccaccg
     2581 ccgtacagga cttcaccgtc gagctgttcg gccgggtgcg gacggacgcc gtgctgcgcg
     2641 ccggactgcg gctgcggccg gagaccgcgc cggggctcgg catctacgca ctggagcagc
     2701 gctggtacgc gctcttcctc gacaaggcgg tcacctgcgg gccgtgcgga ccggccggct
     2761 cgcacgcgcc ggacgccgaa ccgcgccgga cggcccagct gttgacctcg ctcgtggtgg
     2821 gcctgctgca tctgggcggc gaggacggga cgtggtggga cgaggagtcg atcgtgggca
     2881 tgtgggggct gttgccggcc gcgccgagcc ggatcgcggc cgccggcatc ccggcaccgg
     2941 cccagctccc ggcggccggc tgaccggcgc ggccgccacc cgggccggtg ccgcccgcct
     3001 ccccgcgccc ggccctcaca actcgaccag cacggccccg atatgacgcc cctgcgtaag
     3061 ttcgcacagc gcccgcggcg cctgctcgat cccctgaaga agggcgtgcg ggaaggtcag
     3121 cgagccctcc cgcaagccct gcccgaactc cttcgtccac tcctgcggca gatccggatg
     3181 cgcatacagc ccgaagccgc gcagcgtcac ccggcgcgtg atgatcagcc ccgtgtccag
     3241 ctccaccggc gtgagggcgc caccgctccc gcccaactgg ctggagagcg cgccgacgag
     3301 ggcgaaccgc gcatcgggac gggccaccgc cagggccgcc gggagctgtt caccgcccac
     3361 ggtgtccagc agtacatcga tcccgcccgg cgcggcccgg tgcagctggc gctcgatcgg
     3421 tccggcgccg cgcacgatcg tgtcgtcgta gcccaactcg gcgcgcagcc gggccgcctt
     3481 gcgttccgag ccggtgctgc ccaccacccg gcccgccccc agccggcgcg cgagctgccc
     3541 ggccaggctg cccacgccgc ccgccgcgcc ggtgacgaag acggtgtccc ccggccgcac
     3601 ctcggcggcc cggcggagcg cgccccaggc ggtcgccccc tgcgacagat gtgcggcggg
     3661 cgtcggcagg gcgtccgggt cgagccgccg tacccgcgac tcctcgaccg ccgcgaactc
     3721 ccgccagccg tacggatggt ggaccagctc gccgggggcg aagccgccgc cgggtgccgc
     3781 cacgacctcg ccgaccgcgg cgccccacag cgcctgcccc ggcacgaatg agggcatcgg
     3841 cagcggaact ttggtcatct gcgtccgcat cgcagcggtc accgccatca cacgggtacg
     3901 caccagtacc tgccccggct ccggtaccgg caccgggacc tcgactacgg tgaagtgacc
     3961 tgccgtcaat tcgccttcgg cctggtccgc gaggcggacc tcgcgatgta cggccggtat
     4021 ggcggggacg acggtggggg acatcggaca gctcctcgcg ctcgtgacac tcgacggcgt
     4081 cgcaccggac cgcgacgcgg gattcgcgcc accgtcgcgg gcgcgaatca ataccgcatg
     4141 gttggcttct ttatgatgcg gaaatagact gcgcggccac gggcggcggg tcgcactcag
     4201 gcacaacaac tccaagagag catacggagg atgatcggtg gtcaagcagg aacgcgcagt
     4261 gcgcacccga cgggccgtcc tcgaagcggc ggctcatgtc atcggcaccc gcgggtacca
     4321 ggccgccacg atggcagaga tcatccagcg cgccggggtc accaaaggcg ccgtgtactt
     4381 ccatttcacg tccaaggacg cgctggcccg cgcggtcatc acggagcaga ccgatccgtt
     4441 tttgccgcaa gtcagcgaat cccggctgca ggacgccatc gacttcaccc accaggtggc
     4501 gctggccctg cgcagcgatc cgttgctgca ggccggcacc cgcatcgcgg tcgagacgac
     4561 cttcagcgag aacccgctcg tgccgtacca ggcctggacg gacatcatca ccacgatgtt
     4621 caccgaggcc cgggacaacg gggaattact gcccggcgtg gcaccggacc gggcggccga
     4681 gttcttcgtc gccgcctaca tgggcgtgca gttgttctca cgggccgcga ccaaccgcgc
     4741 cgatctcccg gagcgcgtca cggccctgtg gaagcacacc ctcccgggtc tggcgtcgcc
     4801 cggcgcgctg agccacctcg atccgcacgg ccggtgcgtg aaggtcccgg cctgacacca
     4861 ccgttcaccg ccccgacgaa gccggggccc gtccggcact ggaaaacaaa ccacatggtc
     4921 cgtatcttag tgccgcacga acggtcacca cggctcagcc cggcagggcg cgaagagccg
     4981 cacctcagaa aggaacacct atggccagcg ccacgctcac gccgtacttg cgcgatgagc
     5041 gccacgacaa cggaggcgag gacgccaacg gaatccgacc gtccgcgctc accacgacgg
     5101 tgccgcggga gtacgtgcac cgcaccgccg tctccgaggt gttcctgacc aactggcgtc
     5161 aaggcgacgc cgacagctgg gtggtcagcg cgcagtggcc gcgcgcccac accttctacg
     5221 gtccggtcca cgggctgcac gacccgctgc tgctgataga gagcatgcgg caggccggga
     5281 tattactcag ccacgtcgcc cacggcgtgc ccctggacca cccgatcatc tggcagcggg
     5341 tccgctacga cctcttaccg gaggcgctgc gcaccaccga ccggcccgcc gaggtcgaac
     5401 tgcacatcac cgaccacgac atggtccacc gcggcaagcg gctggccagc gcccgccagg
     5461 agttccggat cgtgtgcgac ggcgccgaac tggccacggc ggcgctcgac tactcctgtc
     5521 acagcccggc ggtctaccgc cggctccgcg gcgaatactg cgatctccag ctcgccaact
     5581 cccggaagct gccggttccc gaggccgttc ctccgcagct ggtcgcccgg gaccacgacc
     5641 gcgatgtggc gctctccccc accgaccgcc ccgaccgctg gcaactgcgg gtggacacct
     5701 cgcacccggt gctcttcgac caccccgtcg accacacgcc cggcatgctg atgatcgagg
     5761 ccgcccggca ggccgcacag gcgaccgccc ccggcttcac cctcccggtc tccatggagt
     5821 gctcgttcgc gcgctatgcc gaactcgacg caccgtcctg ggtacaggcg cggaccaccg
     5881 accgggccga cgacggcagc cggcagctca cggtcgacgt cgaacagcac gacaagcccg
     5941 tcgtctccgc gcagatcacc agcgtgccga tgtcctgacc ggccgctgcg ccggcccgcc
     6001 ggccgcctgc cgccacacgc cgcttcaccg cccgcccggc ccctgccggg ccggcccccg
     6061 tacggccccg ccccacactg cccgtgcctc ccccgtcacc gcagtgtgag gcggggcttc
     6121 ggcgcgtcgg tgcgtccggt gggcggggga cggttgcccg cctggtacgg cagcggccag
     6181 ggcgctcccg gccccgcgta cccctgctcc gcggcggcgt gcagcgtcca gtgcgggtcg
     6241 tacagatgcg gcctggccag cgcgcacagg tccgcgcggc cggccagcac cagggagttg
     6301 acgtcgtccc aggaggagat cgcaccgacc gcgatgaccg gcacaccgag ggtgttgcgg
     6361 atgcggtcgg cgaacggggt ctggtaggag cggccgaagt cggggcgctc gtcggggacc
     6421 acctgtccgg tggacacatc gatggcgtcc gcgccgtgcc gtgcgaaggc ctcggcgatg
     6481 gccaccgcgt cccgggccgt ggtgccgccg tccgcccagt ccgtcgcgga gatccggacg
     6541 gtcatcgggc gcccggccgg ccagacggcg cggaccgcgt cgaagacctc cagcgggaag
     6601 cgcagccggg cgtggacgtc gccgccgtag gcgtcggtgc gccggttggt gagcggggag
     6661 aggaagccgg agagcagata gccgtgtgcg cagtggagtt cgagcagatc gaagccggcg
     6721 cgggcggcgg attcggcgga gcggacgaac tgctcgcgta cggtcgcgag ttcgggctcg
     6781 gtcagggcgt gcggaagctg gttgacgccg gcgcggtagg gcagcgggga ggccgccacg
     6841 aggggccagt tgccctccgg gagcggctgg tcgatgccgt cccacatcag ccgggtcgag
     6901 cccttgcggc cggagtgccc gagctggaca ccgatggcgg tgccgggcgc ggaggtgtgg
     6961 acgaagtcgg tgacccggcg ccaggcggtc tcatggcccg gtgcgtacag tcccgtaccg
     7021 ccgggggtga tccgtccctc cgggctgacg cacaccatct ccgtcatcac cagtccggcg
     7081 ccgcccagcg cgcgggcacc gaggtggacg aggtggaagt cgccgggggt gcccgcgccg
     7141 tccaccgcgg agtacatgtc catcggggag accacgacgc ggttgcgcag ggtcagcccg
     7201 cccagccgga acggggtgaa catgggcggc gtggcggact cgatgcccgc ctcacgctcg
     7261 acggccgtga cgaatccggg gtcgcgcagc cgcaggttgt cgtgggtgac ccggcggctg
     7321 cgggtgagga gattgaaggc gaactggtgg ggcggctggt cgaggtaggt ggccagctcc
     7381 tcgaaccagg cgaggctggc gcgggcggcc cgctgggtgg aggcgacgac ggggcggcgc
     7441 tcctcctcgt aggcggccag cgcggaggcg gtgtcgggct gctcctggag gcaggcggcc
     7501 agcgccaggg cgtcctcgac ggcgagcttg gtgccggagc cgatggagaa gtgcgcggtg
     7561 tgcgcggcgt cgccgagcag caccgtattg ccgtgggacc agcgcgcgtt gacgacggta
     7621 cgaaaggcga tccagctgga gttgttggcg cgcagcgacc ggccgcgcag cgcgtccgcg
     7681 aagaccttgg cgcaccactg cgccgattcg cgctcgtcgc agcggtcgag tccggccgcc
     7741 cgccagacct cctcgcgcat ctcgacgatg acggtgctgg cgccgtcggg gtgctccccg
     7801 gaggggctcg ttgcgaggtg gtcggacgag gggcgggcgt agggataggc gtggagctgt
     7861 gcgacgccgt gttcggtctg cgcgatctcg aagcggaagg cgtcgagggc gaagtcggcg
     7921 gcgagccaga tgtagcggca gcggtgggtg gtcagctgcg ggccgaagat gtcggcgtgc
     7981 gcggtgcggg tcgcgctgtg caccccgtcg gcggcgatca ccaggtcgta ggtgcgcgcc
     8041 agttcggcag cgggcggggc ctcggtacgg aagcgcagct cgatgccgag gtcacggcag
     8101 cgctggtgga gtacggccag cagccggcgc cggccgaggg ccgcgaagcc gtgcccgccg
     8161 gaggtcaggg tgcggccccg gtgcacgatg tcgatgtcgt cccagcggac gaactccgcc
     8221 tggagggcgc ggtagacctc cgggtcggcg tgctcgatgc cgccgagggt ctcgtcggag
     8281 agcaccacac cgaatccgaa ggtgtcctcg ggtgcgttgc gctcccatac ggtgatctcg
     8341 cgggtggggt cgaggcgctt gagcagggcc gcggcataga gcccgcccgg ccccccgccg
     8401 atgacggcga cccgcatggc tacttcccct gccacttggg cgtccgcttc tccgtgaagg
     8461 cggcgtggaa ttccgcgtag tcgtcgctgt tcatcagcag cgcctgggtg gcggcgtcca
     8521 tctcgacggc ggcggccagc ggcatgtcga gctccgcggt cagcagcgcc ttggtctggg
     8581 cgtgggccag cgcgggcccc tcggcgagcc gtttggcgag cgccgcggcg gcctcgtcgg
     8641 cccgcccctc ctcggccagc tcactgatca gcccgaggcg ttcggcctcc ggggcacgga
     8701 cggggtcgcc gagcatcagc agccgggtgg catggccgag gccgatgacg cgcggcagga
     8761 gataggccgc gcccatgtcg cccccggaca gcccgacccg ggtgaagagg aaggcgaagc
     8821 gggcggaagg gtcggcgacg cggaagtccg cggccagcgc cagcaccgca ccggccccgg
     8881 ccgcgacccc gtgcacggcc gcgatcaccg ggaacggaca ctcccgcagc gccctgacga
     8941 cctgcccggt catccggttg aagtccagca gctgcgcggt gtccatgccc agggtggcgc
     9001 cgatgatctc ctcgacatcg ccgccggagc agaaaccgcg cccctccccc gcgagcacga
     9061 gggcgcgcac cgagcgctcc cgggagagtt ccgcgagcag atcgcggagg tcggcgtagg
     9121 cctcgaaggt gagggcattg agcttgtccg ggcgcgcgag ggtgacggtg gcgatgccgt
     9181 ccttcctggt cacgcggatg tgctgccagg gctctgtgct gcgggcggat cctgcgaacg
     9241 ggctcatcgg gctgcctgcc ccctttggcc ggtcggccgg tggatgctgt tccggatgcc
     9301 cctcgaagtt atcacccctt catgactacc gtcacgagaa cgcgatatgc cgcgcgtcgc
     9361 cggcggcggt gcgccgccct cgggaccgcg cgggcgaggg tgccgaaggt cccggctccc
     9421 gcatgacgta ggtcttgtgc accccggcca cacgcccctg atcccggcgc cgcgcgcttc
     9481 ctacgctgga tccgaccgaa ccggcctcgg acggccgggc tggctcctgc gaacggatcg
     9541 acggcacatg ctggaacccg cgaccccgac ctggcacatc gcgctgccgc gtagcacgac
     9601 ggccgtcccc atcgcccgcg cgatgatccg ggccgcgctc caggacctgc cggtggcggc
     9661 cgactgtccc accgcgcagc tgctgacctc cgagctggtc agcaacgccc tccagcacac
     9721 ctgcggtacg gagcccatcg agctggtggt ggaggtgcag ccgacgggct gcctgatcga
     9781 ggtgcacgac gacgatccgc tgggcatcga cgtgctgctc gacgaaccgt tcccgctgtc
     9841 agggccggac gatgcggtgt tggtggcggc gcccgatgct tccgggccgg ggcgggcggc
     9901 ggcggggagc gccacggcgg agggggcccc ttcgggggac gaggtgcctg cggtggatgc
     9961 ggggggcagg gtccctgctg tggacggggt tcctgcggcg gatgcggcag acagggtccc
    10021 tgtggtggat gcggtggacg gggcccctgc ggcgggcggg gcgcgtacgg ccgatggtca
    10081 ccgggcggac gatcatcagg cggccgacga ccggcttccg gcgcagcgta cggggggcgt
    10141 cgcgccggcg gatgacgccg ggcgcagcgg cggggacgag ggcggcgacg cccgggagag
    10201 cgcggaggcg ggcggcaccg aggcgggcac gggcacccgg accacggcca cccgggccgc
    10261 ccgcggcacg ccgatcatcc aggccgccga cgccgtcgat gccgcttatg ccgccgaagc
    10321 caccggcctg ggcccgcacg gccacgacca ccaccgccgt ggcctgctgc tgctccgttc
    10381 gctgagctcc gacgcgggct gccgccggac gccgcacggc aaggccgtct ggttcaccct
    10441 tcccgaggtg ccgtggcggc gccggtgagg ccggtgccgg ccccgtccgc cggttcctga
    10501 accgtcggtt cctgagccgc ctgttcctcc tctgcctgcc cgagccgcga acggcgccgc
    10561 ccgtagatcg cgtagaccag cgccccggcc accaggaaac cggcgaactg gaaccaggtc
    10621 gccggaccgg tgccgtagct cagatacaga cagaaggcga tgcccaggag cgggctgacc
    10681 gggaagagcg gcacgcggaa gcggcgcggc agatcggggc gggtgcggcg cagcaccatc
    10741 acgctgaggt tgaccgccac catgaccgcc agcgtcccga tggtcgacag attcatcacc
    10801 gcgtccagcg gcaccaccgc ggccggcacg gcgaagaccg ctgcgacgat ccaggtgttg
    10861 gcgacggggg tggacgtccg gggagagacc cgctcgaaga tccgcgggac cagtccgtcc
    10921 cgcgacatcg acatcaggat gcgggtctgg ccgtacatga ccgcgaggac gaccgagg
//
