LOCUS       NZ_LT907981            10981 bp    DNA     circular CON 12-FEB-2024
DEFINITION  Streptomyces sp. 2323.1 chromosome I.
ACCESSION   NZ_LT907981
VERSION     NZ_LT907981.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. 2323.1
  ORGANISM  Streptomyces sp. 2323.1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2017) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 03:31:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,058
            CDSs (total)                      :: 6,971
            Genes (coding)                    :: 6,808
            CDSs (with protein)               :: 6,808
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 64 of 163
            Pseudo Genes (incomplete)         :: 123 of 163
            Pseudo Genes (internal stop)      :: 17 of 163
            Pseudo Genes (multiple problems)  :: 37 of 163
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:07:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 665689
            Orig. end                         :: 676670
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT907981.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10981
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="5"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10981
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10981
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5981](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5981
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(65..787)
                     /locus_tag="CPH93_RS02355"
                     /old_locus_tag="SAMN06272775_0507"
     CDS             complement(65..787)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608005.1"
                     /locus_tag="CPH93_RS02355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_0507"
                     /product="DUF4232 domain-containing protein"
                     /protein_id="WP_093487671.1"
                     /transl_table=11
                     /translation="MSLTTKTPGRGRRVAAGSLIAVAALGLTACKGGTDAASHPSSAAS
                     PATSSDTGSGSQASGSGHSPGAKPQSSAPGGPRSSSAPSVTPKGSVPGTTQSHSSAMSA
                     SDRCTAAAMSLRLGPADIGAGNIHYSLIFTNKSKKACSLRGFPGVSLINRDGSAVGKPA
                     TRAGGSGGVVRLQPGQSAHAVLHTLNEGVSDTPCWGKSQIVYVYPPGSKESMTTGSGGL
                     RVCGGRFDVTAVQPGALG"
     misc_feature    complement(883..900)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.36"
                     /tool="antismash"
     misc_feature    complement(894..911)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.67"
                     /tool="antismash"
     gene            complement(1310..3751)
                     /locus_tag="CPH93_RS02360"
                     /old_locus_tag="SAMN06272775_0508"
     CDS             complement(1310..3751)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574653.1"
                     /locus_tag="CPH93_RS02360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_0508"
                     /product="MFS transporter"
                     /protein_id="WP_173909697.1"
                     /transl_table=11
                     /translation="MTRTTTDQPAGGAAAIAAAPLVHDPAVGGRRRPGGGIVPVLAFAG
                     IVVAVMQTLLVPVIKDLPVLLNTAPSNATWMLTATLLAGAVATPIMGRLGDLNGKRRML
                     LTSLAIMVVGSLVCGFTDDLVTMIVGRALQGFAMGAIPLGIGIMRDELPRERLGAAMGL
                     MSSSIGVGGGLALPVAALVAQHADWHTLFFGAAGLGVLSILLTLLVVPETAVRAQGRFD
                     VAGALGLSAGLVALLLPITKGSDWGWGSPTTLGLFGLAALILVLWGVLELRRPDPLVDL
                     RTTARREVLLTNLASITVGVAFYAVSLVLPQLLQLPTSTGYGLGQSMVVAGLCMAPLGL
                     TMMCVAPLYARIAARRGPKVSLMLGMLVIAIGYGAGMGLMAAAWQTVIIAVVLGAGIGL
                     AYSSLPALIIGAVDPSETGAANGLNTLMRSIGTSVSSAVIGMVLAHMSTRAGDVTVPTM
                     AGFRVSFLIATAAVLVGVALASFLPSQLKASRGPALVAQSPDGTEDVEQHAVAHGPPGP
                     QAVGTVPVAGTLPGAAAPAEGAEAAGRLAVVGADRVTDHVAGFRGRVADTSGTPVPGAS
                     ITLIDRQGRQAGVTTAGAEGHYALGAPAAGAYVLTAAAPGHAPHAASATCRGAGPSTRV
                     DLVLSGDARLHGTLFGGPEGTPLDGGSVVVTDTRGDVVACTASGADGSWEVAQLPPGAY
                     TLVLSALGHRPQARAWELSGGEAERLDARLQPSATVRGTVRGPGGRPLADAAVTLVEDG
                     TVAGHTVTGPDGVFAFTELTGSHYTLTAVGYPPHAAPISLTGGADALLDLELAQPPATT
                     G"
     gene            complement(4153..4827)
                     /locus_tag="CPH93_RS02365"
                     /old_locus_tag="SAMN06272775_0509"
     CDS             complement(4153..4827)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608007.1"
                     /locus_tag="CPH93_RS02365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_0509"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_096211781.1"
                     /transl_table=11
                     /translation="MAQQDRAIRTRRLILEAAASVFDDLGYDRATIAEVLERAGVTKGA
                     LYFHFASKEQLALAVLDEHVTDIAVEPQKIKLQEFVDAGQVLAYRLRSDPIQRGAARLA
                     VEQGSNHLDRKQGMIAWTRFVEDLLNEARDRGEVLESVVVSETAELFVGAFAGLQLMSN
                     ALTNQADLSHRLTVFFEHTLPSIAVPAVLAKLKLDPERGAELDAALQRTGRASTGVDIA
                     VS"
     misc_feature    4925..4938
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.3"
                     /tool="antismash"
     gene            5001..5981
                     /locus_tag="CPH93_RS02370"
                     /old_locus_tag="SAMN06272775_0510"
     CDS             5001..5981
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608008.1"
                     /locus_tag="CPH93_RS02370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_0510"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_093487665.1"
                     /sec_met_domain="AfsA (E-value: 7.6e-18, bitscore: 63.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDTMHVSGAVQPRAPHQAPLNRNQAASGDHRDPCVPREYVHRPL
                     VDDILVTSWRRRDASHFSLTAQWPHDHGYFTPVHGRHHLILTGETIRQAGLLLCHTELG
                     VPVGHHFILGDLVYTTHPEDLTVGHGPTRLTVDVRCSRMRFRSGTLTSGHFDMTIRRAG
                     RTVATGHSDVTVTSPAVYRRIRGARLTARRVLEPLPAPLPHELTGSAHDSDVLLSPTGR
                     PDRWQLRIAPGHAAMVNPANDHIPGMLLLDAAQQAARALTAPQALVPYAFGTEFRRYAE
                     HGTPCIIEARRVPSALPCTTTIQVTGSQDGEPVFVSTLTALDPRP"
     misc_feature    5799..5801
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6176..6940
                     /locus_tag="CPH93_RS02375"
                     /old_locus_tag="SAMN06272775_0511"
     CDS             6176..6940
                     /NRPS_PKS="Domain: PKS_KR (7-175). E-value: 9.9e-10. Score:
                     30.5. Matches aSDomain:
                     nrpspksdomains_CPH93_RS02375_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608009.1"
                     /locus_tag="CPH93_RS02375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_0511"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_096211782.1"
                     /transl_table=11
                     /translation="MGDLKGKTALVTGASRGIGRGIAQRLAEDGALIAVHYGSNDAAAK
                     ETVEGITRAGGRAFVIGAELGVPGDAEALFAAFDAELAAFGAEPGLDILVNNAALNFPG
                     RIDEVTYEEFDRTIAVNIRAPFFLVQHGLRRMRDGGRIINVSSAVTSTAHPSQIAYSVS
                     KGGVDTLTLTLAKDLGARGITVNTLAPGWVETDVTAARRATPEGRAALAAYSVFNRIGR
                     PGDIADVAAFLASDDSRWITGQRIDVTGGSML"
     aSDomain        6197..6700
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CPH93_RS02375_PKS_KR.1"
                     /evalue="9.90E-10"
                     /label="CPH93_RS02375_PKS_KR.1"
                     /locus_tag="CPH93_RS02375"
                     /protein_end="175"
                     /protein_start="7"
                     /score="30.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGASRGIGRGIAQRLAEDGALIAVHYGSNDAAAKETVEGIT
                     RAGGRAFVIGAELGVPGDAEALFAAFDAELAAFGAEPGLDILVNNAALNFPGRIDEVTY
                     EEFDRTIAVNIRAPFFLVQHGLRRMRDGGRIINVSSAVTSTAHPSQIAYSVSKGGVDTL
                     TLTLA"
     CDS_motif       6203..6268
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CPH93_RS02375_0001"
                     /evalue="8.40E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="CPH93_RS02375"
                     /protein_end="31"
                     /protein_start="9"
                     /score="15.2"
                     /tool="antismash"
                     /translation="LVTGASRGIGRGIAQRLAEDGA"
     gene            complement(6996..7736)
                     /locus_tag="CPH93_RS02380"
                     /old_locus_tag="SAMN06272775_0512"
     CDS             complement(6996..7736)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608010.1"
                     /locus_tag="CPH93_RS02380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_0512"
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="WP_093487661.1"
                     /transl_table=11
                     /translation="MTLTTTKRRARPSGLARRVAAADWPALTGELNELGCALTPRLLDP
                     ADCRALIACYDEDRRFRSTIDMARHRFGSGQYRYFADPPPEPVQALREAFYPHLLTIAR
                     DWADKLGRPAPWPDTLEEWLTLCHDAGQNKSSQILLRYGAGDWNALHRDLFGEMVFPLQ
                     VVIGLDEQGTDYTGGEFLLVEQRPRAQSRGTTTVLPPGHGLIFTTRDRPVRSARGWSAG
                     HVRHGVSTVRSGHRHSLGLVFHDA"
     gene            complement(7865..8374)
                     /locus_tag="CPH93_RS02385"
                     /old_locus_tag="SAMN06272775_0513"
     CDS             complement(7865..8374)
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608011.1"
                     /locus_tag="CPH93_RS02385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_0513"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_096211783.1"
                     /transl_table=11
                     /translation="MTLYTTLDSPLGELLLVGEESATAPGGTALASLSLPGQKGGATVQ
                     DGWTPAPDAFAAIVQQLRAYFDGTLTRFSIEFLTRGTDFQQRVWKALEAVPYGTTTTYG
                     RIAADIGAARGAVRAVGTAIGANPLLIVRPCHRVIAADGSLSGYAAGPARKRKLLGVEG
                     GAPDAA"
     gene            complement(8371..9894)
                     /locus_tag="CPH93_RS02390"
                     /old_locus_tag="SAMN06272775_0514"
     CDS             complement(8371..9894)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608012.1"
                     /locus_tag="CPH93_RS02390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_0514"
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="WP_197708731.1"
                     /transl_table=11
                     /translation="MSNEDSRYEAVRSRDARFDGAFFFAVSTTGIYCRPSCPATTPRRR
                     NVTFFPTSAAAQGSGFRACRRCRPDAVPGSAEWNARADVVGRAMRLISEGVVDREGVRG
                     LAARLGYSARQVQRQLNAELGAGPVALARAQRAHTARILLQTTGLQTAEIAFAAGFASV
                     RQFNDTIKEIYALTPSELRAARPGRTARYGPVAPYATTGVLPLRLAFRGPNAARQLFDH
                     LQRRALTGIEDISGEVGARTYRRTLRLPNGTGIAEVDEATGDGWLNCRLHLTELRDLTT
                     ASQRVRRLFDLDADPYAVAERLGEDPVLARLVAQRPGLRAPGAPDPHELAVRAVLGQQV
                     SLAAGRTLGGALVAAYGEPLPEPVGPLTHLFPRADDLAQASLAELGMPASRRATLRTLS
                     AALADGTVHLDAGADRDQAEQALLGLRGIGPWTAGYIRMRALGDPDVLLSGDVAVRAGM
                     RRAGSSTAALAAHAHGWRPWRSYAMHHFWNVPATEPADIRPPTTESTGP"
     gene            complement(10247..10441)
                     /locus_tag="CPH93_RS02395"
                     /old_locus_tag="SAMN06272775_0515"
     CDS             complement(10247..10441)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608013.1"
                     /locus_tag="CPH93_RS02395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_0515"
                     /product="hypothetical protein"
                     /protein_id="WP_093487655.1"
                     /transl_table=11
                     /translation="MSDGGDNGGNGDGGYGGDSSDSGLPWQPSRRSSSAPDWGVLRRAM
                     RWETFALVLFLVVLAALAS"
     gene            complement(10666..10893)
                     /locus_tag="CPH93_RS02400"
                     /old_locus_tag="SAMN06272775_0516"
     CDS             complement(10666..10893)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537759.1"
                     /locus_tag="CPH93_RS02400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN06272775_0516"
                     /product="hypothetical protein"
                     /protein_id="WP_006608014.1"
                     /transl_table=11
                     /translation="MTEHPPVIVHPPTPSGGRRVTVRGQIVGLAHGRGDVAEFLRRAGV
                     AGPAEDIALDDPRLVEWRGGSLDDWPMPPA"
ORIGIN
        1 gtccgggtcg gtccgccgtc cgagggtcgg cccagggggg cggtccgcgg tccctgtccg
       61 gtcgtcagcc cagggccccg ggctgcacgg ccgtcacgtc gaaccggccg ccgcacaccc
      121 gcaggccgcc gctgcccgtg gtcatcgact ccttggagcc gggcgggtag acgtagacga
      181 tctgcgactt gccccagcac ggggtgtccg agacgccctc gttgagggtg tgcagcacgg
      241 cgtgtgcgct ctgccccggc tggagccgga cgacgccgcc gctgccgccc gcgcgggtgg
      301 cgggcttgcc gaccgccgag ccgtcccggt tgatcagcga caccccgggg aaaccgcgca
      361 gcgaacaggc cttcttgctc ttgttcgtga agatgaggga gtagtggatg ttgcccgcgc
      421 cgatgtcggc ggggcccagg cgcagcgaca tggcggcggc ggtgcagcgg tccgatgccg
      481 acatcgcgga gctgtgggac tgggtggtgc ccgggaccga gcccttcggt gtcacgctgg
      541 gtgccgacga ggaccgggga ccgcccggcg cggaggactg cggcttggcg ccgggcgagt
      601 gtccgctgcc ggacgcctgc gagccactgc cggtgtcgct ggacgtggcg ggggaggcgg
      661 ccgacgaggg gtgggatgcg gcatcggtcc cgcccttgca ggcggtgagc ccgagcgccg
      721 cgacggcgat cagcgatcct gcggcgacac gacggccgcg gccgggcgtc ttggtggtga
      781 gcgacataag tgtccccctc gtctggtagg tgcgagcggt cgtagaaggt gtgcggtccg
      841 tccgttgccc tttccagcgt ggccccggtc actgctggtc cgctaacgag gcactaacac
      901 accgctaacg actgtgtacg ctcggcacgc cgataactcc ggcaaccggt gtgaccagcc
      961 gggattccgg gcgtgccgct cagactcgca gaaattcccc gagttgcgga cagggcacca
     1021 ccgggttcgg ccgttccgcg gaacgggttc ctccgcctca cccctcccga ccgccccacc
     1081 gaccccgagg gctcagccgg tggccggttt tgctacgccc cctgtccgcg gtcggccccg
     1141 atgttcggcg gatcggcaac cggattgccg tagaagtcgc cccacccccg ccagggacgg
     1201 gctacgcccg cccccagggc cagtgagccg gcgcgcgacc gcgcatcgcc cggggcccac
     1261 gggtgcccgc cggggagacg gacggtgggt gcgtcagccg gtggaagcgt cagccggtgg
     1321 tcgccggcgg ctgggcgagc tccaggtcga ggagcgcgtc ggcgccgccg gtgagggata
     1381 tcggggcggc gtgcggcgga tatccgactg cggtgagggt gtagtgactg ccggtcagct
     1441 cggtgaaggc gaagacgccg tccgggccgg tgacggtgtg cccggcgacc gtgccgtcct
     1501 cgacgagggt cacggcggcg tcggccagtg gccgtccgcc cgggccccgc accgtgccgc
     1561 ggaccgtggc agacggctgc agtcgggcgt ccagccgttc cgcctcgccg ccggacagtt
     1621 cccatgcacg ggcctgcggc cggtgcccca gggcgctgag gaccagggtg tacgcgccgg
     1681 gcggcagctg ggcgacctcc cagctcccgt cggcaccgga ggccgtacaa gccaccacat
     1741 cgcccctggt gtcggtgacg acgacgctcc cgccgtccag cggggtgccc tccgggccgc
     1801 cgaacagggt gccgtgcagc cgggcgtcgc cgctgagtac caggtcgacc cgggtcgagg
     1861 ggcccgcgcc gcggcaggtg gcagaggccg catgcggggc gtggccgggc gcggcggcgg
     1921 tcagaacata ggcgccggcc gccggagcac cgagcgcata gtgcccctcg gcgccggccg
     1981 tggtgacgcc cgcctggcgg ccctgccgat ctatcagggt gatgctcgca ccggggaccg
     2041 gagtgccaga ggtgtccgcc acccggccgc ggaatccggc cacgtggtcg gtgacgcggt
     2101 cggccccaac gactgcgagc cgaccggctg cctccgcccc ttcggccggt gcggcagctc
     2161 cgggcagcgt gcccgccacc ggcacagtgc ccacggcctg cggccccggc gggccgtgcg
     2221 caacagcatg ctgctccacg tcctccgtac cgtccggact ctgcgcaacc agggccggac
     2281 cacgggacgc cttcagctgc gacggcagga aggacgcaag agcgacgccg acgaggaccg
     2341 cggccgtggc gatcaggaag gagacccgga agccggccat cgtgggcacc gtgacatctc
     2401 cggcgcgcgt ggacatgtgg gccagcacca tgccgatgac cgcgctggac accgaggtgc
     2461 cgatcgaacg catcagggtg ttcaggccgt tggccgcgcc ggtctcggag gggtcgacgg
     2521 cgccgatgat cagggcgggc agcgaggagt acgccaggcc gatgccggcg ccgaggacca
     2581 ccgcgatgat gacggtctgc caggcggcag ccatcaggcc catgcccgcg ccgtagccga
     2641 tcgcgatgac cagcatgccc agcatcaggg agaccttggg gccgcgccgg gcggcgatcc
     2701 gcgcatacag cggggcgacg cacatcatcg tcaggcccag cggtgccatg cacagtccgg
     2761 ccaccaccat ggactggccg aggccgtagc cggtcgacgt gggcagctgg agcagctgcg
     2821 gcagcaccag ggagacggcg tagaacgcca caccgaccgt gatcgaggcg aggttggtca
     2881 gcagcacctc acgccgggcg gtggtccgca gatcgaccag cggatcgggc cggcgcagct
     2941 ccagcacgcc ccacagcacc aggatcagtg ccgcgaggcc gaacaggccg agggtggtgg
     3001 gtgaacccca gccccagtcg ctgcccttgg tgatgggcag cagcagggcc accagaccgg
     3061 ccgacagccc gagggcaccg gccacgtcga agcgcccctg ggcacgtacc gcggtctccg
     3121 gtacgacgag gagggtgagc aatatcgaca gcacgccgag cccggcggcg ccgaagaaca
     3181 gggtgtgcca gtcggcgtgt tgggcgacca gggccgcgac cggcagcgcc agcccgccgc
     3241 cgacgccgat cgaggagctc atcaacccca tggccgcacc gagccgttcg cgcggcagct
     3301 cgtcgcgcat gatgccgatg ccgaggggaa tggcgcccat ggcgaagccc tggagcgccc
     3361 ggcccacgat catggtgacc aggtcgtcgg tgaatccgca gaccagcgag ccgacgacca
     3421 tgatggcgag gctggtcagc agcattcgcc gcttgccgtt cagatcgccg aggcgtccca
     3481 tgatcggcgt cgcgacggca ccggcgagga gggtggcggt cagcatccac gtggcgttgc
     3541 tgggtgccgt gttgagcagt accggcaggt ccttgatgac cggtacgagc agcgtctgca
     3601 tcaccgcgac gacaatgccg gcgaaggcca gcaccggaac gatgccaccg ccgggtctac
     3661 ggcgcccgcc cacggccggg tcgtggacca ggggcgctgc ggcgatggcc gccgcccctc
     3721 cggctggctg gtcagtcgtc gtccgggtca tgcgtagggc ctccagggcg gcagagcgga
     3781 gtgcggtgtc gtcagggctg gcagagcagc agggatgagc agcaggcgaa cccagtgaac
     3841 cgcaccccac tgacaatccc gctcggaagc gatcgcgtgc atcgcgccct cgctcgcggg
     3901 gcaccgccca ctggtgcacc cgtcaacagc gagaggcgcc gcggttcttc cgcgacgcct
     3961 cccgctcggc ccacactcca ctgacctgca cacttcgcgc acgactcatc cacatgccgg
     4021 cattccacac cgactacgcg gtatttttca gccgccctga ttgccctgga ccatccgaac
     4081 tgcccggcac cttccgacaa cgccgactcg tctcgactcg acgcggctcc atccggcccc
     4141 gcccgccccg gctcaactca ccgcaatatc gaccccggtg gacgcccggc ccgtccgctg
     4201 cagcgcggca tcgagctcgg cgccgcgctc cgggtccagt ttgagcttgg cgagcacggc
     4261 gggcaccgcg atgctgggga gggtgtgctc gaagaacacc gtcagacggt ggctgaggtc
     4321 cgcctgattg gtcagcgcgt tcgacatcaa ctgcaggccg gcgaacgctc cgacgaagag
     4381 ctcggcggtc tcggacacca cgacgctctc cagcacctcg ccgcgatccc tcgcctcgtt
     4441 cagcagatcc tcgacgaacc gcgtccaggc gatcatcccc tgcttgcgat ccaggtggtt
     4501 ggagccctgc tcgacggcca gccgggccgc tccgcgctgg atcgggtcgc tgcgcagccg
     4561 gtacgcgagc acctgccccg cgtcgacgaa ctcctgcagt ttgatcttct gcggctccac
     4621 ggcgatgtcc gtcacatgct catcgagcac ggcgagggcg agctgttcct tggaggcgaa
     4681 gtggaaatac aaggcgccct tcgtcacgcc ggcgcgctca aggacctcgg cgatggtcgc
     4741 acggtcgtag ccgaggtcgt cgaagaccga cgcggccgcc tccaggatca gtcgccgggt
     4801 ccgaatagcg cggtcctgtt gtgccacagg acgcctcctg tctcttgaag ttttcccgtc
     4861 tgcatcagtt agctaccgct caagtcgccg ctgagccagc tacttactcc gattcgtcgc
     4921 ccggcattga aaataaaacc ggatggtacg tatcttaaca gcgccggacc tcattccatc
     4981 tgagacgcct gggggcagtc atgagcgaca cgatgcacgt cagcggcgct gttcagccca
     5041 gggctccaca tcaggcgccg ctgaaccgca atcaggccgc ttccggagac caccgagacc
     5101 cctgcgtccc ccgggaatac gtacaccgcc cgctcgtcga cgacattctg gtcacctcat
     5161 ggcgtcggcg ggacgcctcc catttctcgc tgaccgccca gtggccgcac gaccacgggt
     5221 atttcacgcc ggttcacggc cgccaccacc tcattctcac cggcgagacc atccgccagg
     5281 cagggctgct gctgtgccac accgagctcg gcgtaccggt cgggcaccat ttcatcctcg
     5341 gcgacctggt gtacacaact caccccgagg acctgacggt tggccatgga ccgacccggc
     5401 tgacggtcga tgtccggtgc agccggatgc gcttccggtc cggcacgctc accagtggcc
     5461 acttcgacat gaccatccgc agggcggggc ggaccgtcgc gacgggccac tccgacgtca
     5521 cggtcacctc gccggccgtc taccgccgga tacgcggtgc ccggctgacc gcgcgtcgcg
     5581 tcctcgaacc gctccccgcg cccctcccgc acgagctgac cggcagcgcc catgacagcg
     5641 atgtcctgct ctcccccacc ggccggcccg accgctggca gctgcgtatc gcacccggcc
     5701 atgccgcgat ggtcaacccc gccaacgacc acatcccggg aatgctgctg ctcgacgccg
     5761 cgcagcaggc cgcccgcgcg ctgaccgcgc cccaggcctt agtcccgtac gccttcggca
     5821 cggaattccg ccgctacgcg gaacacggca ccccctgcat catcgaggca cggcgcgtcc
     5881 cgtccgcgct cccctgcacc acgacgatcc aggtcaccgg ctcacaggac ggcgagccgg
     5941 tcttcgtctc caccctgacg gcgctggacc cgcgcccctg agcccaccgc tcgcggacgc
     6001 gttctgggca tcgccccgac ggaccccaac accccgcacg tcaccggacg tcgtgccgtg
     6061 cggggcacgc acagacccgg ccggccgccg ggcacccggc ggccacccgc aagacccccg
     6121 ttcacgtcgt ggcgcgcagc atgcccacct caggtaccag aaaggccttc agatcatggg
     6181 tgacctcaag ggaaagaccg cgctggtgac gggagccagc cggggcatcg gccggggcat
     6241 cgcccagcgc ctcgccgagg acggggccct gatcgccgtc cactacggca gcaatgacgc
     6301 cgcggccaag gaaacggtcg agggcatcac gcgcgcgggc ggccgggcct tcgtgatcgg
     6361 agcggaactg ggagttcccg gtgacgccga agccctcttc gccgctttcg acgccgaact
     6421 cgccgccttc ggtgcggaac caggcctcga tattctcgtc aacaacgcgg cgctcaactt
     6481 ccccgggcgc atagacgagg tgacctacga ggagttcgac cgcaccatcg cggtcaacat
     6541 cagggccccg ttcttcctcg tccagcacgg tctgcgccgg atgcgggacg gcggacgcat
     6601 catcaacgtc tcgtcggcgg tgacgagtac ggcgcacccg tcgcagatcg cctacagcgt
     6661 ctccaagggc ggcgtggaca ccctcacact caccctcgcc aaggacctcg gcgcgcgcgg
     6721 gatcacggtg aacaccctcg cccccggctg ggtcgagacc gatgtgaccg cggcacggcg
     6781 ggccacgccc gaggggcggg ctgcgctggc ggcctattcg gtgttcaacc ggatcggccg
     6841 gcccggggac atcgcggacg tcgccgcgtt cctggcctcg gacgactccc gctggatcac
     6901 cggccagcgc atcgacgtca ccggtggctc gatgctctga ccccgccagg ggccaccctc
     6961 ggcccggcgc aaggccatgg cgtcgtcagg catcgtcagg cgtcgtggaa gaccaggccc
     7021 agggagtggc ggtggcccga gcggacggtg ctcacaccgt ggcgtacgtg gccggccgac
     7081 cagccgcggg ccgagcggac gggacggtcg cgggtcgtga agatcaggcc gtggcccggt
     7141 ggcaggacgg tggtggtgcc gcgggactgt gcgcgcggcc gctgctcgac gaggaggaac
     7201 tcgcccccgg tgtagtccgt gccctgctcg tccaggccga tgacgacctg gagcgggaag
     7261 accatctccc cgaagaggtc acggtgcagc gcgttccagt cgcccgcccc gtaacgcagc
     7321 aggatttggg aggacttgtt ctgcccggcg tcgtggcaca gggtgagcca ctcctccagg
     7381 gtgtccggcc agggcgccgg ccggccgagc ttgtccgccc agtcccgggc gatcgtcagc
     7441 aggtgtggat agaacgcctc gcgcagggcc tgcacgggtt cgggcggcgg gtcggcgaag
     7501 tagcggtact ggcccgagcc gaaacggtgc cgcgccatgt cgatggtcga gcggaagcgc
     7561 cggtcctcgt cgtagcaggc gatgagggcg cggcagtcgg ccggatcgag caggcgtggg
     7621 gtcaatgcgc agcccagctc gttgagttcg ccggtcaggg ccggccagtc ggcggccgcg
     7681 acgcgccggg cgagcccgga ggggcgggct cggcgcttgg tggtggtgag ggtcatgagg
     7741 tcctccaagg gggcgtcggg gcggctcacg gggcgcgcag acgtggccgg caagccccgc
     7801 agacgccccc tacgggcagc cggctccgcc gcccggcgga tacggcgccc cgtgcggaac
     7861 gggctcaggc ggcgtccggg gcgccgccct cgacgccgag gagcttccgc ttgcgcgcgg
     7921 gcccggccgc gtagccggag agggatccgt ccgcggcgat cacacggtga cagggccgga
     7981 cgatgagcag cgggttcgcg ccgatcgccg ttccgacggc gcgcaccgca ccgcgcgccg
     8041 caccgatgtc ggcggcgatc ctcccgtacg tggtcgtggt gccgtacgga accgcctcca
     8101 gggccttcca cacccgctgc tggaagtccg tgccccgggt gaggaattcg atgctgaagc
     8161 gggtcagcgt cccgtcgaag tacgcgcgca gctgctggac gatcgcggcg aaggcgtcgg
     8221 gggcgggcgt ccagccgtcc tggacggtgg caccgccctt ctggccgggc agggacagcg
     8281 aagcgagcgc ggtgccgccc ggtgcggtgg ccgattcctc acccaccagg agaagttcgc
     8341 ccaacgggct gtcgagggtg gtgtagagcg tcatggtcct gtgctttccg tagtgggtgg
     8401 gcggatgtcg gccggctccg tggccggcac gttccagaag tggtgcatcg cgtacgagcg
     8461 ccaggggcgc cagccgtggg cgtgcgccgc cagagcagcc gtcgaggacc cagcccggcg
     8521 cattccggcc cgtacggcca catcgccgct cagcaggaca tcggggtccc cgagggcgcg
     8581 catccggatg tagccggcgg tccacgggcc gatgccgcgc agaccgagca gcgcctgctc
     8641 cgcctggtcc cggtcggctc cggcgtccag gtggaccgtg ccgtcggcga gtgcggcgct
     8701 gagggtgcgc agggtcgccc gccgcgacgc gggcatgccg agttcggcga gcgaggcctg
     8761 tgccaggtca tcggcacggg ggaagaggtg ggtgagcgga ccgaccggtt ccggcagcgg
     8821 ttctccgtac gcggcaacca gcgcgccgcc gagggtgcgg ccggccgcga gcgagacctg
     8881 ctgaccgagc acggcacgga cggcgagctc gtgcgggtcg ggtgcgcccg gcgcccgcag
     8941 tccggggcgc tgtgccacca gccgggccag aacggggtct tcgccgagcc gctcggccac
     9001 cgcatacggg tcggcgtcca gatcgaagag gcggcgcacc cgctggctcg cggtggtgag
     9061 atcgcgcagc tcggtgaggt gcagccggca gttcagccag ccgtcaccgg tcgcctcgtc
     9121 gacctcggcg atgccggttc cgttcggcag ccgcagagtg cgccggtagg tccgggcgcc
     9181 gacctcaccg ctgatgtcct cgatgccggt gagcgcgcgc cgctggagat ggtcgaagag
     9241 ctgccgcgcc gcattcgggc cgcggaaggc caggcgcaac ggcagcactc ccgtcgtggc
     9301 atacggcgcc accggaccgt atcgggccgt tctgccgggg cgcgcggcgc gcagctcact
     9361 cggggtgagc gcgtagatct ccttgatcgt gtcgttgaac tggcgcacgc tggcgaagcc
     9421 cgccgcgaag gcgatctcgg ctgtctgcag gccggtggtc tgtagcagga tccgggcggt
     9481 gtgggcgcgc tgcgcccgcg ccagcgccac gggaccggcg cccagctcgg cgttcagctg
     9541 ccgctggacc tgccgggcgc tgtagccgag ccgggccgcg agcccgcgga cgccttcccg
     9601 gtcgaccaca ccctcgctga tcagccgcat ggcccgcccg acgacatcgg cgcgggcatt
     9661 ccactccgcg gagccgggga ccgcatccgg gcggcagcgc cgacaggcgc ggaagccgga
     9721 gccctgggcg gcggcagagg tcgggaagaa ggtgacgttc cggcgccggg gcgtggtggc
     9781 ggggcagctc ggccggcagt agatcccggt cgtggacacc gcgaagaaga aggcgccgtc
     9841 gaatctggcg tcccggctgc gtaccgcctc gtacctgctg tcctcgttgc tcacggagtc
     9901 cactgtgcag caggtcaggc ccgggcgctg gcggaaatcg gacggcgcgt tcgggggggc
     9961 cgagggacag ggaggtgtcg gccggggcgt cgggccgccc cggggagagg agctgggtgc
    10021 cgccgcctcg tggggagccg ggtgccgtcg ccctatggaa ggcctggcgc cgttctcctc
    10081 aggggacggg atgccgtcac ggacgggccg gccagggtcc ccggacaacg ccggtcagcg
    10141 atgcggcagt tggcaacaga cggttagcgg tcagcagcag gggcggtggt catcgccgga
    10201 cgggccatcg ccctgcgccg ggcgcccgca ccggctcgac gccgcgttat gacgccagcg
    10261 ccgcaaggac caccaggaag aggaccaggg cgaacgtctc ccagcgcatc gccctccgca
    10321 gcacccccca gtccggggcg ctcgacgacc ggcggctggg ctgccatggc aagccgctgt
    10381 cggaactgtc gccgccgtag ccgccgtccc cgttaccccc gttgtcccca ccgtcactca
    10441 tgtcgcgctc ccgctctcct gccctcacct cacgggccga acagcttgtc gcccgccgcc
    10501 cgtcagcgta tgtcatacgg ccagggggtg gcggagcgcg gccgacgacg gcggacgcgt
    10561 gcccgggacg acgcagcacc ccgcacggcg acgcacgcag aacgatgtga agcacccggc
    10621 acggtgacct gccgagcgcg atggccgcat cggtgacccg ccgcttcagg ccggcggcat
    10681 cggccagtca tccagcgagc cgccgcgcca ttcgacgagg cgcgggtcat cgagggcgat
    10741 gtcctcggcc gggccggcga cgcccgcacg gcggagaaac tcggcgacat cgccgcggcc
    10801 gtgggccaga ccgacgatct gcccacgtac ggtcacccgc cggccaccgg acggcgtcgg
    10861 gggatgcacg atgacaggcg gatgctcggt catgcctcca gcgtgcgccg ggctccgcgc
    10921 tcgcgcatgt cgacgaccgc cgggggcgtc acccgccgcc gggtatgtcg gtcggcgcgg
    10981 g
//
