GCF_900215385.1 - 7 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_OBEE01000022.1 (Stenotrophomonas sp. CC120223-11)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 8.1	RiPP-like	30,534	41,379

NZ_OBEE01000001.1 (Stenotrophomonas sp. CC120223-11)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 18.1	lassopeptide	61,901	84,455
Region 18.2	NRP-metallophore,NRPS	313,721	362,088	High	2,3-dihydroxybenzoylserine	NRPS:Type I

NZ_OBEE01000033.1 (Stenotrophomonas sp. CC120223-11)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 23.1	RiPP-like	8,419	19,294

NZ_OBEE01000002.1 (Stenotrophomonas sp. CC120223-11)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 35.1	lanthipeptide-class-ii	7,325	30,222

NZ_OBEE01000026.1 (Stenotrophomonas sp. CC120223-11)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 39.1	terpene-precursor	44,555	57,563

NZ_OBEE01000028.1 (Stenotrophomonas sp. CC120223-11)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 42.1	arylpolyene	6,777	50,379	Low	aryl polyenes	other:other

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 8.1	RiPP-like	30,534	41,379
Region 18.1	lassopeptide	61,901	84,455
Region 18.2	NRP-metallophore,NRPS	313,721	362,088	High	2,3-dihydroxybenzoylserine	NRPS:Type I
Region 23.1	RiPP-like	8,419	19,294
Region 35.1	lanthipeptide-class-ii	7,325	30,222
Region 39.1	terpene-precursor	44,555	57,563
Region 42.1	arylpolyene	6,777	50,379	Low	aryl polyenes	other:other

No secondary metabolite regions were found in these records:: NZ_OBEE01000054.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000051.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000050.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000052.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000049.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000048.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000047.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000021.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000020.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000019.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000012.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000046.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000016.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000009.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000041.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000011.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000023.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000003.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000004.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000014.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000018.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000007.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000005.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000015.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000008.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000006.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000040.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000010.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000044.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000025.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000024.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000039.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000042.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000017.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000027.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000053.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000038.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000036.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000029.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000030.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000031.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000035.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000043.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000037.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000013.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000032.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000034.1 (Stenotrophomonas sp. CC120223-11); NZ_OBEE01000045.1 (Stenotrophomonas sp. CC120223-11)

Compact view

NZ_OBEE01000022.1 - Region 1 - RiPP-like

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Location: 30,534 - 41,379 nt. (total: 10,846 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000043.5	0.37	PKS	curacin A	Moorea producens 19L
BGC0001484.3	0.33	other (other)	2'-chloropentostatin, 2'-amino-2'-deoxyadenosine	Actinomadura sp. ATCC 39365
BGC0002000.4	0.32	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0000527.5	0.31	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0002571.2	0.31	NRPS	pyoverdine DC3000	Pseudomonas syringae pv. tomato str. DC3000
BGC0000453.5	0.31	NRPS	valinomycin	Streptomyces tsusimaensis
BGC0001789.3	0.31	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000536.3	0.31	ribosomal	nisin Q	Lactococcus lactis
BGC0000535.4	0.31	ribosomal	nisin A	Lactococcus lactis subsp. lactis
BGC0000537.3	0.31	ribosomal	nisin U	Streptococcus uberis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000043.5	0.37	PKS	curacin A	Moorea producens 19L
BGC0001484.3	0.33	other (other)	2'-chloropentostatin, 2'-amino-2'-deoxyadenosine	Actinomadura sp. ATCC 39365
BGC0002000.4	0.32	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0000527.5	0.31	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0002571.2	0.31	NRPS	pyoverdine DC3000	Pseudomonas syringae pv. tomato str. DC3000
BGC0000453.5	0.31	NRPS	valinomycin	Streptomyces tsusimaensis
BGC0001789.3	0.31	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000536.3	0.31	ribosomal	nisin Q	Lactococcus lactis
BGC0000535.4	0.31	ribosomal	nisin A	Lactococcus lactis subsp. lactis
BGC0000537.3	0.31	ribosomal	nisin U	Streptococcus uberis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.37 out of 20.47

Confidence: medium

Additional weak hits (2):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.11 out of 18.57

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.64 out of 32.93

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_OBEE01000001.1 - Region 1 - lassopeptide

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Location: 61,901 - 84,455 nt. (total: 22,555 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lasso peptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

CRO53_RS00360: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000577.3	0.50	ribosomal	sphingonodin II	Sphingobium japonicum UT26S
BGC0001786.3	0.50	ribosomal	subterisin	Novosphingobium subterraneum
BGC0000573.3	0.49	ribosomal	caulonodin I, caulonodin II	Caulobacter sp. K31
BGC0001779.5	0.44	ribosomal	siamycin	Streptomyces nodosus
BGC0002015.4	0.44	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0002307.3	0.43	ribosomal	humidimycin	Streptomyces humidus
BGC0000519.4	0.41	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000616.4	0.40	ribosomal	amylocyclicin	Bacillus velezensis FZB42
BGC0000528.5	0.40	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001887.3	0.39	ribosomal	huazacin	Bacillus thuringiensis serovar huazhongensis BGSC 4BD1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000577.3	0.50	ribosomal	sphingonodin II	Sphingobium japonicum UT26S
BGC0001786.3	0.50	ribosomal	subterisin	Novosphingobium subterraneum
BGC0000573.3	0.49	ribosomal	caulonodin I, caulonodin II	Caulobacter sp. K31
BGC0001779.5	0.44	ribosomal	siamycin	Streptomyces nodosus
BGC0002015.4	0.44	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0002307.3	0.43	ribosomal	humidimycin	Streptomyces humidus
BGC0000519.4	0.41	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000616.4	0.40	ribosomal	amylocyclicin	Bacillus velezensis FZB42
BGC0000528.5	0.40	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001887.3	0.39	ribosomal	huazacin	Bacillus thuringiensis serovar huazhongensis BGSC 4BD1

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lasso peptide predictions

Lassopeptide(s) for CRO53_RS21815

CRO53_RS21815

Leader:

MNELIELGVVAGQQNPEDQGVGGELIELGSVVSETK

Full core:

AHNHGTLWDGVFPTTSP

Putative macrolactam:

AHNHGTLWD

antiSMASH-DB 4.0 matches for CRO53_RS21815

100.0%

NC_011071.1and 41 others:

NZ_AP021867.1 Lassopeptides: GJU96_RS20605_lassopeptideNZ_CP011010.1 Lassopeptides: VN11_RS22530_lassopeptideNZ_CP011305.1 Lassopeptides: YH67_RS21020_lassopeptideNZ_CP015612.1 Lassopeptides: A7326_RS21600_lassopeptideNZ_CP027562.1 Lassopeptides: C6Y55_RS23185_lassopeptideNZ_CP029773.1 Lassopeptides: DM611_RS22725_lassopeptideNZ_CP031058.1 Lassopeptides: DUW70_RS11975_lassopeptideNZ_CP031167.1 Lassopeptides: MG068_RS21080_lassopeptideNZ_CP037858.1 Lassopeptides: EZ304_RS20935_lassopeptideNZ_CP040432.1 Lassopeptides: FEO95_RS22840_lassopeptideNZ_CP040438.1 Lassopeptides: FEO89_RS21625_lassopeptideNZ_CP040439.1 Lassopeptides: FEO88_RS23220_lassopeptideNZ_CP043578.1 Lassopeptides: F0Q13_RS11365_lassopeptideNZ_CP046588.1 Lassopeptides: GOC52_RS10870_lassopeptideNZ_CP047310.1 Lassopeptides: GS396_RS20180_lassopeptideNZ_CP060259.1 Lassopeptides: H7F32_RS11180_lassopeptideNZ_CP067993.1 Lassopeptides: JJL50_RS10815_lassopeptideNZ_CP077679.1 Lassopeptides: KS456_RS10490_lassopeptideNZ_CP080573.1 Lassopeptides: K1516_RS11235_lassopeptideNZ_CP090418.1 Lassopeptides: LYZ64_RS11980_lassopeptideNZ_CP096975.1 Lassopeptides: M1L61_RS12085_lassopeptideNZ_CP104169.1 Lassopeptides: NDR96_RS09175_lassopeptideNZ_CP106759.1 Lassopeptides: N8888_RS09665_lassopeptideNZ_CP120400.1 Lassopeptides: PVE91_RS09960_lassopeptideNZ_JABDRV010000002.1 Lassopeptides: HRL60_RS08285_lassopeptideNZ_JADCQA010000003.1 Lassopeptides: IMX13_RS03920_lassopeptideNZ_JAEDVS010000016.1 Lassopeptides: JAK50_RS09365_lassopeptideNZ_JAEDVW010000040.1 Lassopeptides: JAK53_RS10000_lassopeptideNZ_JAEDWA010000005.1 Lassopeptides: JAK63_RS01795_lassopeptideNZ_JAEDWK010000019.1 Lassopeptides: JAK51_RS11395_lassopeptideNZ_JAEDWU010000002.1 Lassopeptides: JAK37_RS06750_lassopeptideNZ_JAKJQX010000001.1 Lassopeptides: MT466_RS00850_lassopeptideNZ_JAQQGI010000001.1 Lassopeptides: PQS91_RS01155_lassopeptideNZ_JVHP01000037.1 Lassopeptides: ADI44_RS25485_lassopeptideNZ_JVPM01000019.1 Lassopeptides: ADH50_RS20965_lassopeptideNZ_JVUI01000038.1 Lassopeptides: ADG58_RS23065_lassopeptideNZ_OBEE01000001.1 Lassopeptides: CRO53_RS21815_lassopeptideNZ_OU943334.1 Lassopeptides: LNN95_RS11880_lassopeptideNZ_RHPZ01000001.1 Lassopeptides: EGJ03_RS21120_lassopeptideNZ_RHQE01000001.1 Lassopeptides: EGJ89_RS22430_lassopeptideNZ_SOCO01000001.1 Lassopeptides: N440_RS20340_lassopeptide

Lassopeptides: SMAL_RS21110_lassopeptide

1

AHNHGTLWDGVFPTTSP

17

AHNHGTLWDGVFPTTSP

1

AHNHGTLWDGVFPTTSP

17

88.2%

NZ_CP058243.1:

Lassopeptides: AD14011_RS10295_lassopeptide

1

AHNHGTLWDGVFPTTSP

17

AH HGTLWDG+FPTTSP

1

AHGHGTLWDGIFPTTSP

17

61.1%

NZ_CP049017.1:

Lassopeptides: G4Q83_RS07645_lassopeptide

1

AHNHGTLWDGVF-PTTSP

17

AHNHGT + F TTSP

1

AHNHGTFLEASFIATTSP

18

with 5 more sequence matches

29.2%

NZ_CP033362.1:

Lassopeptides: EB232_RS01185_lassopeptide

6

TLWDGVFPTTSP

17

T DGVFP +P

6

TALDGVFPIHTP

17

22.2%

NZ_CP044492.1and 1 other:

NZ_CP066294.2 Lanthipeptides: IGS65_RS08185_lanthipeptide

Lanthipeptides: FSA31_RS00605_lanthipeptide

8

WDGVFPTTS

16

W GV PT S

4

WQGVVPTVS

12

22.2%

NZ_CP049223.1and 4 others:

NZ_CP049225.1 Lanthipeptides: G6Z85_RS06555_lanthipeptideNZ_CP049228.1 Lanthipeptides: G6Z83_RS06555_lanthipeptideNZ_JAKWZB010000003.1 Lanthipeptides: MMO78_RS01405_lanthipeptideNZ_PNGO01000002.1 Lanthipeptides: CJ224_RS02730_lanthipeptide

Lanthipeptides: G6Z86_RS06445_lanthipeptide

8

WDGVFPTTS

16

W GV PT S

4

WQGVLPTVS

12

22.2%

NZ_FNUH01000004.1and 1 other:

NZ_FOGM01000011.1 Lanthipeptides: BMX20_RS07220_lanthipeptide

Lanthipeptides: BLU50_RS05815_lanthipeptide

7

LWDGVFPTTS

16

W GV PT S

3

FWQGVVPTVS

12

13.9%

NZ_CP015883.1and 1 other:

NZ_JAASJP010000011.1 Lanthipeptides: allorf_43013_43183_lanthipeptide

Lanthipeptides: allorf_0205278_0205448_lanthipeptide

3

NHGTLWDGVF

12

N+G +W+ VF

16

NNGCMWNTVF

25

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 25.59 out of 25.75

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.71 out of 23.79

Confidence: medium

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.58 out of 21.84

Confidence: medium

Additional weak hits (7):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.76 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 24.07 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.46 out of 29.38

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 20.78 out of 29.25

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 25.78 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 26.30 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 20.01 out of 33.14

Confidence: weak

Lasso peptides

RREFinder

TFBS Finder

Core peptide predictions

CRO53_RS21815 - Class II: Cleavage pHMM score: 10.60; RODEO score: 22; Monoisotopic mass: 1817.8 Da; Molecular weight: 1818.9 Da; Number of bridges: 0

RRE predictions

: RRE type: Lasso_Fused_RRE; Location: 5 - 77; Score: 47.5

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
MatP	Cell cycle regulator
MexT	Global virulence regulator

NZ_OBEE01000001.1 - Region 2 - NRP-metallophore,NRPS

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Location: 313,721 - 362,088 nt. (total: 48,368 nt) Show pHMM detection rules used

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

CRO53_RS01495: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CRO53_RS01535: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

CRO53_RS01555: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

Hydrolases: dehalogenase hydrolase

CRO53_RS01410: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

CRO53_RS01510: isochorismatase

smcogs: SMCOG1027: isochorismatase

Isomerases: isomerase

CRO53_RS01520: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase

CRO53_RS01500: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CRO53_RS01515: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002689.2	1.33	NRPS	2,3-dihydroxybenzoylserine	Stenotrophomonas maltophilia K279a
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.69	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.68	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.63	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000343.5	0.62	NRPS	enterobactin	Pseudomonas sp. J465

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002689.2	0.87	NRPS	2,3-dihydroxybenzoylserine	Stenotrophomonas maltophilia K279a
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.68	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001641.4	0.65	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000343.5	0.65	NRPS	enterobactin	Pseudomonas sp. J465

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.12 out of 23.79

Confidence: medium

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 18.67 out of 20.01

Confidence: medium

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 28.02 out of 33.14

Confidence: medium

Additional weak hits (21):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.36 out of 18.57

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.44 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.65 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.23 out of 29.38

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.81 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.08 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.16 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.59 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 26.18 out of 32.74

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 23.91 out of 30.97

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 19.21 out of 31.73

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 24.29 out of 31.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.96 out of 26.06

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 27.70 out of 39.62

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 25.50 out of 39.62

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.58 out of 30.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.91 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.56 out of 28.14

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 23.92 out of 39.62

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 27.05 out of 39.62

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.26 out of 33.14

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 313720 - 362088:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CRO53_RS01500: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (457..845): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWELHFFTSGEHNLYGPTEAAVDVSY
Nearest Stachelhaus code(s):: Ser DVWHFSLVDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

CRO53_RS01515: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (39..444): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: SASLFPMSSPGGGLQVGGAQQVFGMAEGLVNYTR
Nearest Stachelhaus code(s):: diOH-Bz PMPAQGVVNK (94% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ArgR	Regulator of arginine biosynthesis genes
IolR	Regulator of the inositol catabolism
ZuR variant 2	Zinc-responsive repressor

NZ_OBEE01000033.1 - Region 1 - RiPP-like

Download region GenBank file

Download region SVG

Location: 8,419 - 19,294 nt. (total: 10,876 nt) Show pHMM detection rules used

Region on contig edge.

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001962.3	0.27	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0000188.5	0.23	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0001650.4	0.23	NRPS	Le-pyrrolopyrazines	Lysobacter enzymogenes
BGC0000452.5	0.21	NRPS	tyrocidine	Brevibacillus brevis NBRC 100599
BGC0000651.5	0.20	terpene	KS-505a	Streptomyces argenteolus
BGC0001283.3	0.18	PKS	Bisenarsan	Streptomyces lividans 1326

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001962.3	0.27	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0000188.5	0.23	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0001650.4	0.23	NRPS	Le-pyrrolopyrazines	Lysobacter enzymogenes
BGC0000452.5	0.21	NRPS	tyrocidine	Brevibacillus brevis NBRC 100599
BGC0000651.5	0.20	terpene	KS-505a	Streptomyces argenteolus
BGC0001283.3	0.18	PKS	Bisenarsan	Streptomyces lividans 1326

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.74 out of 20.47

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.64 out of 26.85

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.19 out of 19.26

Confidence: medium

Additional weak hits (4):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.48 out of 27.78

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.71 out of 39.62

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.56 out of 28.19

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
RutR	Regulator of the pyrimidine and purine metabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_OBEE01000002.1 - Region 1 - lanthipeptide-class-ii

Download region GenBank file

Download region SVG

Location: 7,325 - 30,222 nt. (total: 22,898 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase

CRO53_RS02035: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.52	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002703.2	0.49	ribosomal	archalan α	Halorussus salinus
BGC0000554.4	0.48	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0002346.3	0.47	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000503.5	0.46	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001579.4	0.46	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0002698.2	0.43	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001576.3	0.43	ribosomal	divamide A	Prochloron didemni
BGC0002316.2	0.41	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002704.2	0.41	ribosomal	archalan β	Halorussus salinus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.52	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002703.2	0.49	ribosomal	archalan α	Halorussus salinus
BGC0000554.4	0.48	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0002346.3	0.47	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000503.5	0.46	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001579.4	0.46	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0002698.2	0.43	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001576.3	0.43	ribosomal	divamide A	Prochloron didemni
BGC0002316.2	0.41	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002704.2	0.41	ribosomal	archalan β	Halorussus salinus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for CRO53_RS02035

CRO53_RS02030

Leader:

MDLNDDVVSGWLNGSDDVDGCANPAGSLFIYGA

Core:

AATESALTSASDTLITGGGTECTTTMSVVNHCLCC

Core with dehydrations:

AADhbEDhaALDhbDhaADhaDDhbLIDhbGGGDhbECysDhbDhbDhbMDhaVVNHCysLCysCys

antiSMASH-DB 4.0 matches for CRO53_RS02030

100.0%

NZ_CP015612.1and 17 others:

NZ_CP027562.1 Lanthipeptides: C6Y55_RS08375_lanthipeptideNZ_CP028358.1 Lanthipeptides: C9J74_RS08630_lanthipeptideNZ_CP040435.1 Lanthipeptides: FEO92_RS08800_lanthipeptideNZ_CP040438.1 Lanthipeptides: FEO89_RS08350_lanthipeptideNZ_CP040439.1 Lanthipeptides: FEO88_RS08780_lanthipeptideNZ_CP056088.1 Lanthipeptides: HUZ96_RS08220_lanthipeptideNZ_CP090418.1 Lanthipeptides: LYZ64_RS08310_lanthipeptideNZ_CP109812.1 Lanthipeptides: FQS62_RS08595_lanthipeptideNZ_DS999412.1 Lanthipeptides: SSKA14_RS15505_lanthipeptideNZ_JAEDUZ010000008.1 Lanthipeptides: JAK52_RS03215_lanthipeptideNZ_JAEDWK010000010.1 Lanthipeptides: JAK51_RS04155_lanthipeptideNZ_JAJNMF010000022.1 Lanthipeptides: LQ423_RS11465_lanthipeptideNZ_JVUI01000059.1 Lanthipeptides: ADG58_RS15825_lanthipeptideNZ_LR134301.1 Lanthipeptides: EL200_RS07985_lanthipeptideNZ_OBEE01000002.1 Lanthipeptides: CRO53_RS02030_lanthipeptideNZ_RHQE01000015.1 Lanthipeptides: EGJ89_RS13150_lanthipeptideNZ_SOCO01000001.1 Lanthipeptides: N440_RS05690_lanthipeptide

Lanthipeptides: A7326_RS08120_lanthipeptide

1

AATESALTSASDTLITGGGTECTTTMSVVNHCLCC

35

AATESALTSASDTLITGGGTECTTTMSVVNHCLCC

1

AATESALTSASDTLITGGGTECTTTMSVVNHCLCC

35

97.1%

NZ_CP011010.1:

Lanthipeptides: VN11_RS08230_lanthipeptide

1

AATESALTSASDTLITGGGTECTTTMSVVNHCLCC

35

AATESALT+ASDTLITGGGTECTTTMSVVNHCLCC

1

AATESALTAASDTLITGGGTECTTTMSVVNHCLCC

35

97.1%

NZ_CP043578.1and 3 others:

NZ_CP067993.1 Lanthipeptides: JJL50_RS07700_lanthipeptideNZ_JVHP01000063.1 Lanthipeptides: ADI44_RS21105_lanthipeptideNZ_OU943334.1 Lanthipeptides: LNN95_RS09005_lanthipeptide

Lanthipeptides: F0Q13_RS08255_lanthipeptide

1

AATESALTSASDTLITGGGTECTTTMSVVNHCLCC

35

ATESALTSASDTLITGGGTECTTTMSVVNHCLCC

1

GATESALTSASDTLITGGGTECTTTMSVVNHCLCC

35

with 16 more sequence matches

Only the first 10 are displayed here.

72.3%

NZ_CP047310.1and 4 others:

NZ_CP104169.1 Lanthipeptides: NDR96_RS06465_lanthipeptideNZ_JABDRV010000001.1 Lanthipeptides: HRL60_RS01085_lanthipeptideNZ_JAEDVS010000025.1 Lanthipeptides: JAK50_RS15965_lanthipeptideNZ_JVPM01000038.1 Lanthipeptides: ADH50_RS19075_lanthipeptide

Lanthipeptides: GS396_RS07720_lanthipeptide

2

ATESALTSASDTLITGGGTECTTTMSVVNHCLCC

35

ATESALTSASDTLITGGGTECTTTMSVVNHCLCC

14

ATESALTSASDTLITGGGTECTTTMSVVNHCLCC

47

37.1%

NZ_CP011305.1and 19 others:

NZ_CP031058.1 Lanthipeptides: DUW70_RS15590_lanthipeptideNZ_CP031167.1 Lanthipeptides: MG068_RS07815_lanthipeptideNZ_CP040436.1 Lanthipeptides: FEO91_RS08085_lanthipeptideNZ_CP046588.1 Lanthipeptides: GOC52_RS08100_lanthipeptideNZ_CP060259.1 Lanthipeptides: H7F32_RS08235_lanthipeptideNZ_CP071784.1 Lanthipeptides: J3U96_RS13765_lanthipeptideNZ_CP077679.1 Lanthipeptides: KS456_RS07940_lanthipeptideNZ_CP096975.1 Lanthipeptides: M1L61_RS08375_lanthipeptideNZ_CP104071.1 Lanthipeptides: K7565_RS07800_lanthipeptideNZ_CP104287.1 Lanthipeptides: K7567_RS07875_lanthipeptideNZ_CP104324.1 Lanthipeptides: K7569_RS08320_lanthipeptideNZ_JACLBK010000002.1 Lanthipeptides: ID844_RS05155_lanthipeptideNZ_JAEDVP010000027.1 Lanthipeptides: JAK38_RS15550_lanthipeptideNZ_JAEDVW010000007.1 Lanthipeptides: JAK53_RS03160_lanthipeptideNZ_JAEDWD010000009.1 Lanthipeptides: JAK45_RS02000_lanthipeptideNZ_JAFLWT010000006.1 Lanthipeptides: J1F00_RS06425_lanthipeptideNZ_JAQQGI010000014.1 Lanthipeptides: PQS91_RS16925_lanthipeptideNZ_KB907502.1 Lanthipeptides: G370_RS0114170_lanthipeptideNZ_RHPZ01000014.1 Lanthipeptides: EGJ03_RS16365_lanthipeptide

Lanthipeptides: YH67_RS07965_lanthipeptide

21

ECTTTMSVVNHCLCC

35

ECTTT+SV+NHCLCC

3

ECTTTVSVINHCLCC

17

36.2%

NZ_CP007597.1:

Lanthipeptides: DX03_RS09815_lanthipeptide

2

ATESALTSASDTLITGGGTECTTTMSVVNHCLCC

35

AT++ALT+ + T++TGG CTTTMSV+NHC+CC

25

ATDAALTAMAPTMVTGGTGNCTTTMSVINHCMCC

58

29.3%

NZ_CP069396.1:

Lanthipeptides: JRI60_RS03735_lanthipeptide

1

AATESALTSASDTLIT

16

AATE+ALT ASD L+T

12

AATENALTDASDALVT

27

28.8%

NZ_CP069338.1:

Lanthipeptides: JQX13_RS07220_lanthipeptide

2

ATESALTSASDTLITGGGTECTTTMSVVNHCLCC

35

ATE+ALT+++D L T G + CT ++ H CC

24

ATEAALTNSTDALYT-GCSSCTGSL----HSYCC

52

26.4%

NZ_CP093340.1:

Lanthipeptides: MNO14_RS04685_lanthipeptide

1

AATESALTSASDTLITGGGTECTT-TMSVVNHC

32

AATE+A+T S L+ T+C++ T S+ HC

24

AATEAAMTDKSFLLL----TQCSSCTASIPAHC

52

24.4%

NZ_CP029194.1and 1 other:

NZ_CP059991.1 Lanthipeptides: H4W23_RS35675_lanthipeptide

Lanthipeptides: DEJ46_RS04155_lanthipeptide

1

AATESALTSASDTLITG

17

AA E+ALT A++ L+TG

12

AAGEAALTDATEALLTG

28

24.4%

NZ_JH941044.1:

Lanthipeptides: Y19_RS0126875_lanthipeptide

1

AATESALTSASDTLITG

17

AA E+ALT A++ L TG

12

AAGEAALTDATEALFTG

28

22.9%

NZ_CP089924.1:

Lanthipeptides: LVB77_RS02735_lanthipeptide

16

TGGGTECTTTMSVVNHC

32

+G CT+ SV NHC

2

SGASVNCTSGSSVSNHC

18

22.0%

NZ_CP010407.1and 4 others:

NZ_CP010407.1 Lanthipeptides: SVTN_RS37515_lanthipeptideNZ_CP010407.1 Lanthipeptides: SVTN_RS37515_lanthipeptideNZ_CP010407.1 Lanthipeptides: SVTN_RS37515_lanthipeptideNZ_JACHJY010000017.1 Lanthipeptides: GGE06_RS39715_lanthipeptide

Lanthipeptides: SVTN_RS37515_lanthipeptide

1

AATESALTSASDTLITG

17

AA E+ALT ++ L+TG

12

AAGEAALTDGTEALLTG

28

allorf_016795_016875

Leader:

MSARPLIPCRYRAAHAA

Core:

HPRACRSAA

Core with dehydrations:

HPRACysRDhaAA

antiSMASH-DB 4.0 matches for allorf_016795_016875

39.1%

NZ_CP028358.1and 7 others:

NZ_CP040435.1 Lanthipeptides: allorf_1856776_1856916_lanthipeptideNZ_CP056088.1 Lanthipeptides: allorf_1814243_1814383_lanthipeptideNZ_CP109812.1 Lanthipeptides: allorf_1800751_1800891_lanthipeptideNZ_JAEDUZ010000008.1 Lanthipeptides: allorf_126406_126546_lanthipeptideNZ_LR134301.1 Lanthipeptides: allorf_1747066_1747206_lanthipeptideNZ_OBEE01000002.1 Lanthipeptides: allorf_016795_016875_lanthipeptideNZ_RHQE01000015.1 Lanthipeptides: allorf_094530_094610_lanthipeptide

Lanthipeptides: allorf_1803368_1803508_lanthipeptide

1

HPRACRSAA

9

HPRACRSAA

15

HPRACRSAA

23

34.8%

NZ_CP011305.1and 5 others:

NZ_CP046588.1 Lanthipeptides: allorf_1748975_1749091_lanthipeptideNZ_CP047310.1 Lanthipeptides: allorf_1658784_1658924_lanthipeptideNZ_CP096975.1 Lanthipeptides: allorf_1778660_1778776_lanthipeptideNZ_JAEDVP010000027.1 Lanthipeptides: allorf_23874_23990_lanthipeptideNZ_RHPZ01000014.1 Lanthipeptides: allorf_016179_016295_lanthipeptide

Lanthipeptides: allorf_1722617_1722733_lanthipeptide

2

PRACRSAA

9

PRACRSAA

16

PRACRSAA

23

allorf_022043_022156

Leader:

MHGRILPPAPAFRVTRIQPAARLRPIVAG

Core:

MCTRERIR

Core with dehydrations:

MCysDhbRERIR

antiSMASH-DB 4.0 matches for allorf_022043_022156

100.0%

NZ_CP015612.1and 6 others:

NZ_CP027562.1 Lanthipeptides: allorf_1787381_1787494_lanthipeptideNZ_CP043578.1 Lanthipeptides: allorf_1781995_1782108_lanthipeptideNZ_CP090418.1 Lanthipeptides: allorf_1744347_1744460_lanthipeptideNZ_JAEDWK010000010.1 Lanthipeptides: allorf_0542429_0542542_lanthipeptideNZ_OBEE01000002.1 Lanthipeptides: allorf_022043_022156_lanthipeptideNZ_RHQE01000015.1 Lanthipeptides: allorf_089249_089362_lanthipeptide

Lanthipeptides: allorf_1746482_1746595_lanthipeptide

1

MCTRERIR

8

MCTRERIR

1

MCTRERIR

8

allorf_026037_026213

Leader:

MRVCRGGEFAGG

Core:

GGGQAVARTRREYVPVGSVAPSMARTVLATACPPPHHSLRRIDAAK

Core with dehydrations:

GGGQAVARDhbRREYVPVGDhaVAPDhaMARDhbVLADhbACysPPPHHDhaLRRIDAAK

antiSMASH-DB 4.0 matches for allorf_026037_026213

100.0%

NZ_OBEE01000002.1:

Lanthipeptides: allorf_026037_026213_lanthipeptide

1

GGGQAVARTRREYVPVGSVAPSMARTVLATACPPPHHSLRRIDAAK

46

GGGQAVARTRREYVPVGSVAPSMARTVLATACPPPHHSLRRIDAAK

1

GGGQAVARTRREYVPVGSVAPSMARTVLATACPPPHHSLRRIDAAK

46

91.3%

NZ_CP011010.1:

Lanthipeptides: allorf_1781844_1782020_lanthipeptide

1

GGGQAVARTRREYVPVGSVAPSMARTVLATACPPPHHSLRRIDAAK

46

G GQAVARTRREYVPVGSVAPSMARTVLATACPPPHHS RR AAK

1

GRGQAVARTRREYVPVGSVAPSMARTVLATACPPPHHSFRRRYAAK

46

89.1%

NZ_JAJNMF010000022.1:

Lanthipeptides: allorf_11422_11598_lanthipeptide

1

GGGQAVARTRREYVPVGSVAPSMARTVLATACPPPHHSLRRIDAAK

46

G GQ VARTRREYVPVGSVAPSMARTVLATACPPPHHS RR AAK

1

GRGQPVARTRREYVPVGSVAPSMARTVLATACPPPHHSFRRRYAAK

46

with 3 more sequence matches

23.3%

NZ_RHQE01000015.1:

Lanthipeptides: allorf_086168_086377_lanthipeptide

1

GGGQAVARTRREYVPVGSVAPSMARTVL

28

G + R R +YVPV SVA SM T L

1

GRADRLCRGRCKYVPVSSVAASMRLTPL

28

13.0%

NZ_VDYW01000008.1:

Lanthipeptides: allorf_137107_137307_lanthipeptide

27

VLATACPPPHHSL

39

+L CP H+SL

14

ILLNCCPSTHNSL

26

11.3%

NZ_JAAXRC010000001.1:

Lanthipeptides: allorf_597683_597922_lanthipeptide

9

TRREYVPVGSVAPSM

23

T RE+ PVG P +

18

TPREFTPVGRPKPDL

32

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 21.78 out of 29.72

Confidence: medium

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 27.95 out of 32.74

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 23.47 out of 28.19

Confidence: medium

Additional weak hits (6):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.46 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.61 out of 29.38

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.79 out of 29.65

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.77 out of 23.43

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.18 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.00 out of 24.78

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

CRO53_RS02030 - Class II

Cleavage pHMM score: -6.70

RODEO score: 0

Monoisotopic mass: 3231.5 Da

Molecular weight: 3233.6 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 3251.6 Da N = 1; 3269.6 Da N = 2; 3287.7 Da N = 3; 3305.7 Da N = 4; 3323.7 Da N = 5; 3341.7 Da N = 6; 3359.7 Da N = 7; 3377.8 Da N = 8

allorf_016795_016875 - Class II

Cleavage pHMM score: -7.20

RODEO score: 0

Monoisotopic mass: 949.5 Da

Molecular weight: 950.1 Da

Number of bridges: 1

allorf_022043_022156 - Class II

Cleavage pHMM score: -4.20

RODEO score: 0

Monoisotopic mass: 1045.5 Da

Molecular weight: 1046.3 Da

Number of bridges: 1

allorf_026037_026213 - Class II

Cleavage pHMM score: -3.60

RODEO score: 0

Monoisotopic mass: 4698.6 Da

Molecular weight: 4701.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4719.4 Da N = 1; 4737.4 Da N = 2; 4755.4 Da N = 3; 4773.5 Da N = 4; 4791.5 Da N = 5

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsR	Pleiotropic regulatory for antibiotic production
FuR variant 2	Ferric Uptake Regulator
NtrC	Nitrogen regulatory protein C
ZuR variant 1	Zinc-responsive repressor

NZ_OBEE01000026.1 - Region 1 - terpene-precursor

Download region GenBank file

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Location: 44,555 - 57,563 nt. (total: 13,009 nt) Show pHMM detection rules used

Region on contig edge.

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: synthetase

CRO53_RS19325: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000640.3	0.32	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000630.3	0.31	terpene	(2R,3S,3'S)-2-hydroxyastaxanthin	Paracoccus haeundaensis
BGC0000641.3	0.30	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000639.3	0.30	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000642.3	0.30	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0000638.3	0.29	terpene	carotenoid	Pantoea ananatis
BGC0000643.3	0.29	terpene	carotenoid	Brevundimonas vesicularis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000640.3	0.32	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000630.3	0.31	terpene	(2R,3S,3'S)-2-hydroxyastaxanthin	Paracoccus haeundaensis
BGC0000641.3	0.30	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000639.3	0.30	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000642.3	0.30	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0000638.3	0.29	terpene	carotenoid	Pantoea ananatis
BGC0000643.3	0.29	terpene	carotenoid	Brevundimonas vesicularis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

CRO53_RS19325: PT_FPPS_like

Domain 1 (32 - 292): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: DMAPP, IPP → FPP

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (4):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.54 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.42 out of 18.57

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 20.39 out of 27.04

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.88 out of 20.01

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description

NZ_OBEE01000028.1 - Region 1 - arylpolyene

Download region GenBank file

Download region SVG

Location: 6,777 - 50,379 nt. (total: 43,603 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase reductase

CRO53_RS19625: alkyl hydroperoxide reductase subunit

smcogs: SMCOG1176: alkyl hydroperoxide reductase subunit

CRO53_RS19650: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

CRO53_RS19700: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acyltransferase

CRO53_RS19770: acyltransferase

smcogs: SMCOG1141: acyltransferase

Hydrolases: hydrolase

CRO53_RS19655: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Ligases: ligase synthase

CRO53_RS19695: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CRO53_RS19710: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CRO53_RS19745: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

CRO53_RS19750: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000837.4	0.65	other (other)	APE Vf	Aliivibrio fischeri ES114
BGC0002008.3	0.63	other (other)	aryl polyenes	Xenorhabdus doucetiae
BGC0000836.5	0.62	other (other)	APE Ec	Escherichia coli CFT073
BGC0002276.2	0.60	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001164.3	0.59	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002510.2	0.50	PKS	youssoufene A1, youssoufene B1, youssoufene B2, youssoufene B3, youssoufene B4	Streptomyces sp.
BGC0001895.4	0.50	PKS	huanglongmycin A, huanglongmycin B, huanglongmycin C	Streptomyces sp. CB09001
BGC0002000.4	0.49	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0000840.5	0.49	other (other)	xanthomonadin I	Xanthomonas oryzae pv. oryzae
BGC0001723.5	0.49	PKS	oviedomycin	Streptomyces antibioticus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000837.4	0.65	other (other)	APE Vf	Aliivibrio fischeri ES114
BGC0002008.3	0.63	other (other)	aryl polyenes	Xenorhabdus doucetiae
BGC0000836.5	0.62	other (other)	APE Ec	Escherichia coli CFT073
BGC0002276.2	0.60	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001164.3	0.59	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002510.2	0.50	PKS	youssoufene A1, youssoufene B1, youssoufene B2, youssoufene B3, youssoufene B4	Streptomyces sp.
BGC0001895.4	0.50	PKS	huanglongmycin A, huanglongmycin B, huanglongmycin C	Streptomyces sp. CB09001
BGC0002000.4	0.49	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0000840.5	0.49	other (other)	xanthomonadin I	Xanthomonas oryzae pv. oryzae
BGC0001723.5	0.49	PKS	oviedomycin	Streptomyces antibioticus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.60 out of 19.26

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.99 out of 20.47

Confidence: medium

Additional weak hits (6):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.18 out of 26.06

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.23 out of 27.55

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.57 out of 26.33

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.63 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.73 out of 28.19

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.74 out of 27.04

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

CRO53_RS19750: X

AMP-binding (124..344): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: THQ-----LFRWVAFSSGGTEVYGSSETGGIAWR
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
VqsM	Regulator of quorum-sensing signalling systems