LOCUS       NZ_FZOF01000074         4883 bp    DNA     linear   CON 02-JAN-2025
DEFINITION  Actinacidiphila glaucinigra strain CGMCC 4.1858, whole genome
            shotgun sequence.
ACCESSION   NZ_FZOF01000074
VERSION     NZ_FZOF01000074.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinacidiphila glaucinigra
  ORGANISM  Actinacidiphila glaucinigra
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Actinacidiphila.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2017) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900188405.1-RS_2025_01_02
            Annotation Date                   :: 01/02/2025 02:14:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,793
            CDSs (total)                      :: 8,707
            Genes (coding)                    :: 8,547
            CDSs (with protein)               :: 8,547
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 1 (5S, 16S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 47 of 160
            Pseudo Genes (incomplete)         :: 136 of 160
            Pseudo Genes (internal stop)      :: 6 of 160
            Pseudo Genes (multiple problems)  :: 29 of 160
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:25:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4883
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FZOF01000074.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     aSDomain        complement(<1..140)
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43700_PKS_PP.1"
                     /evalue="4.40E-11"
                     /label="CHF15_RS43700_PKS_PP.1"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="1628"
                     /protein_start="1581"
                     /score="35.0"
                     /tool="antismash"
                     /translation="LAALPQDERAEAVLDVVRGEVAGVLGHGSVDQVPADRAFKELGFD
                     SL"
     aSModule        complement(<1..4877)
                     /complete
                     /domains="nrpspksdomains_CHF15_RS43700_PKS_KS.1"
                     /domains="nrpspksdomains_CHF15_RS43700_PKS_AT.1"
                     /domains="nrpspksdomains_CHF15_RS43700_PKS_DH.1"
                     /domains="nrpspksdomains_CHF15_RS43700_PKS_KR.1"
                     /domains="nrpspksdomains_CHF15_RS43700_PKS_PP.1"
                     /locus_tags="CHF15_RS43700"
                     /monomer_pairings="mal -> ccmal"
                     /tool="antismash"
                     /type="pks"
     source          1..4883
                     /db_xref="taxon:235986"
                     /mol_type="genomic DNA"
                     /note="contig: Ga0079895_174"
                     /organism="Actinacidiphila glaucinigra"
                     /strain="CGMCC 4.1858"
                     /type_material="type strain of Streptomyces glauciniger"
     gene            complement(<1..>4883)
                     /locus_tag="CHF15_RS43700"
                     /old_locus_tag="SAMN05216252_1741"
     region          1..4883
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..4883
                     /SMILES="C=CC(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..4883
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<0:4883](-)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..4883)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS             complement(<1..4883)
                     /EC_number="2.3.1.-"
                     /EC_number="6.4.-.-"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (2-403). E-value:
                     1e-172. Score: 566.7. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43700_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (513-793). E-value: 3e-103.
                     Score: 337.2. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43700_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (865-1025). E-value: 2.2e-63.
                     Score: 205.5. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43700_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (1316-1495). E-value: 7.8e-59.
                     Score: 190.6. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43700_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (1581-1628). E-value: 4.4e-11.
                     Score: 35.0. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43700_PKS_PP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060787.1"
                     /locus_tag="CHF15_RS43700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216252_1741"
                     /product="type I polyketide synthase"
                     /protein_id="WP_143681805.1"
                     /sec_met_domain="ketoacyl-synt (E-value: 1e-85, bitscore:
                     273.4, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 2.9e-219, bitscore:
                     714.2, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 5e-105, bitscore: 337.2,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 1.9e-66, bitscore:
                     209.5, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVGGGDAISPFPQGRGWDLDALFDPDPDGRGTSYTQSGGFLHDAG
                     RFDPAFFGISPREAVAMDPQQRLLLETSWEAFERAGIDPAAMRGSRTGVFAGIMYHDYA
                     TRITSVPDGVEGYLGTGNSGSIASGRVSYAFGLEGPAVTVDTACSSSLVALHWAIQALR
                     NGECTMALAGGVTVMATPGTFTEFSRQRGLAPDGRIKSFAAAADGTSWAEGAGMLLVER
                     LSDARRNGHPVLAVVRGSAINQDGASNGLTAPNGPSQQRVIRAALASAGLSGDEIDAVE
                     AHGTGTTLGDPIEAQALLATYGQEHSDEQPLWIGSIKSNMGHTQAAAGVAGIIKMIMAI
                     RHGVLPRTLHVDEPTPHVDWSEGAVSLLTDSVPWPETGRPRRAGISSFGISGTNAHTII
                     EQAPDDLAAITKARPAPAAPATTALPYVLSAKTEDALRDQAARLRTHLLEEAPEIGTAD
                     VAYSLATRRAAFQQRASVVAADRDELLRGLSALAEGHGAAGLVRGSSSTTGKQAFLFTG
                     QGSQRLGMGRELYAAYPVFAKALDAVGERLGLDVLFGDDAEALDRTEFAQPALFAVEVA
                     LFRLLESWGVKPDYVSGHSIGEIAAAHVAGVLSLEDACTLVEARGRLMQALPTGGVMIA
                     VQASEDEVLPLLTERVSIAAVNGPQAVVIAGDEDAAVAIVESFGDRKSKRLQVSHAFHS
                     PHMDGMLDAFREVVSGLSFEEPRIAVVSNLTGAVVTDEMASPEFWVRHVREAVRFLDGV
                     RALEAAGVATYLELGPDGVLSAMAQECLAQEGATFAPALRGGRPEVGTLTAALAQAYTA
                     GVTVDWEAHFARTGSRQVELPTYAFQREWYWLESGGAQAGPGDATGFGLGAADHPLLGA
                     AVELPDSDGVVFTGRLSLTTHPWLADHAVMGSVLLPGTAFVELAVRAGELVGCEVLEDL
                     TLEAPLVLPEHGGVQLRLSVAAADGEGRRALVLHSRVEDGSWVRHAAGVLAGRAAEPVF
                     DLAVWPPVGAEPVAIDGLYEGLVEAGFGYGPTFQGLRSVWRRGEDVFAEVRLPESAESE
                     AGAFGVHPALLDAALHALGVSGTDGEAKLPFSWSGVTLHAVGASEVRVRLSRTGRDVVA
                     LELADAEGVPVASVAGLVLRAFAPEQFTGPGAGQGDALFRVEWSPVPAGGSVDDWKLAS
                     AGLQTVEAESGAVPAVLVVPCVADEPVPDVAGAVHAETGRVLALVQEWLGDERFAESRL
                     VFLTRGAVAAVPGDEVADLVHAPVWGLVRSAQSENPGRFGLVDLDGEIAPEAVASALAT
                     GEPEIALRAGAFLAPRLARAVANPDAEARALEGTVLVTGASGALGGLFARHLVSEYGVR
                     DLLLVSRRGEAAPGAADLTAELDGLGASVSWAACDVADRDSLAAVLDAIPAERPLTAVV
                     HTAGVLDDGVIASLDQERLSGVLHPKVDAAWNLHQLTQDMDLSAFVLFSSAAGVFGGAG
                     QANYAAANVFLDALAAHRRSQGLAATSLAWGLWDGGMAGEVARSGRGGVDALTEEEGLA
                     LFDTANATQAPVLVPMKLDLAALRAQAGSGMLPPLLSGLVRVPTRRAAKAGSAAAGKLA
                     RQLAALPQDERAEAVLDVVRGEVAGVLGHGSVDQVPADRAFKELGFDSL"
     aSDomain        complement(399..935)
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43700_PKS_KR.1"
                     /evalue="7.80E-59"
                     /label="CHF15_RS43700_PKS_KR.1"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="1495"
                     /protein_start="1316"
                     /score="190.6"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="GTVLVTGASGALGGLFARHLVSEYGVRDLLLVSRRGEAAPGAADL
                     TAELDGLGASVSWAACDVADRDSLAAVLDAIPAERPLTAVVHTAGVLDDGVIASLDQER
                     LSGVLHPKVDAAWNLHQLTQDMDLSAFVLFSSAAGVFGGAGQANYAAANVFLDALAAHR
                     RSQGLAATSLAWGLWD"
     CDS_motif       complement(444..548)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43700_0012"
                     /evalue="1.80E-19"
                     /label="PKSI-KR_m4"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="1480"
                     /protein_start="1445"
                     /score="62.3"
                     /tool="antismash"
                     /translation="AFVLFSSAAGVFGGAGQANYAAANVFLDALAAHRR"
     CDS_motif       complement(576..605)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43700_0011"
                     /evalue="3.60E-03"
                     /label="PKSI-KR_m3"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="1436"
                     /protein_start="1426"
                     /score="10.8"
                     /tool="antismash"
                     /translation="PKVDAAWNLH"
     CDS_motif       complement(831..932)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43700_0010"
                     /evalue="1.20E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="1351"
                     /protein_start="1317"
                     /score="21.2"
                     /tool="antismash"
                     /translation="TVLVTGASGALGGLFARHLVSEYGVRDLLLVSRR"
     aSDomain        complement(1809..2288)
                     /ASF="catalytic triad H,G,P found: False"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43700_PKS_DH.1"
                     /evalue="2.20E-63"
                     /label="CHF15_RS43700_PKS_DH.1"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="1025"
                     /protein_start="865"
                     /score="205.5"
                     /tool="antismash"
                     /translation="HPLLGAAVELPDSDGVVFTGRLSLTTHPWLADHAVMGSVLLPGTA
                     FVELAVRAGELVGCEVLEDLTLEAPLVLPEHGGVQLRLSVAAADGEGRRALVLHSRVED
                     GSWVRHAAGVLAGRAAEPVFDLAVWPPVGAEPVAIDGLYEGLVEAGFGYGPTFQGL"
     CDS_motif       complement(2130..2204)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43700_0009"
                     /evalue="2.20E-09"
                     /label="PKSI-DH_m2"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="918"
                     /protein_start="893"
                     /score="29.8"
                     /tool="antismash"
                     /translation="WLADHAVMGSVLLPGTAFVELAVRA"
     aSDomain        complement(2505..3344)
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43700_PKS_AT.1"
                     /evalue="3.00E-103"
                     /label="CHF15_RS43700_PKS_AT.1"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="793"
                     /protein_start="513"
                     /score="337.2"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFTGQGSQRLGMGRELYAAYPVFAKALDAVGERLGLDVLFGDDAE
                     ALDRTEFAQPALFAVEVALFRLLESWGVKPDYVSGHSIGEIAAAHVAGVLSLEDACTLV
                     EARGRLMQALPTGGVMIAVQASEDEVLPLLTERVSIAAVNGPQAVVIAGDEDAAVAIVE
                     SFGDRKSKRLQVSHAFHSPHMDGMLDAFREVVSGLSFEEPRIAVVSNLTGAVVTDEMAS
                     PEFWVRHVREAVRFLDGVRALEAAGVATYLELGPDGVLSAMAQECLAQEGATFAPALR"
     CDS_motif       complement(2637..2669)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43700_0008"
                     /evalue="1.20E-03"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="749"
                     /protein_start="738"
                     /score="12.3"
                     /tool="antismash"
                     /translation="WVRHVREAVRF"
     CDS_motif       complement(2886..2924)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43700_0007"
                     /evalue="4.80E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="666"
                     /protein_start="653"
                     /score="13.4"
                     /tool="antismash"
                     /translation="AAVNGPQAVVIAG"
     CDS_motif       complement(3009..3131)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43700_0006"
                     /evalue="2.80E-24"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="625"
                     /protein_start="584"
                     /score="77.5"
                     /tool="antismash"
                     /translation="GVKPDYVSGHSIGEIAAAHVAGVLSLEDACTLVEARGRLMQ"
     CDS_motif       complement(3141..3206)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43700_0005"
                     /evalue="7.00E-05"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="581"
                     /protein_start="559"
                     /score="15.9"
                     /tool="antismash"
                     /translation="LDRTEFAQPALFAVEVALFRLL"
     CDS_motif       complement(3294..3341)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43700_0004"
                     /evalue="8.00E-06"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="530"
                     /protein_start="514"
                     /score="18.3"
                     /tool="antismash"
                     /translation="FTGQGSQRLGMGRELY"
     aSDomain        complement(3675..4877)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43700_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="1.00E-172"
                     /label="CHF15_RS43700_PKS_KS.1"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="403"
                     /protein_start="2"
                     /score="566.7"
                     /tool="antismash"
                     /translation="GGGDAISPFPQGRGWDLDALFDPDPDGRGTSYTQSGGFLHDAGRF
                     DPAFFGISPREAVAMDPQQRLLLETSWEAFERAGIDPAAMRGSRTGVFAGIMYHDYATR
                     ITSVPDGVEGYLGTGNSGSIASGRVSYAFGLEGPAVTVDTACSSSLVALHWAIQALRNG
                     ECTMALAGGVTVMATPGTFTEFSRQRGLAPDGRIKSFAAAADGTSWAEGAGMLLVERLS
                     DARRNGHPVLAVVRGSAINQDGASNGLTAPNGPSQQRVIRAALASAGLSGDEIDAVEAH
                     GTGTTLGDPIEAQALLATYGQEHSDEQPLWIGSIKSNMGHTQAAAGVAGIIKMIMAIRH
                     GVLPRTLHVDEPTPHVDWSEGAVSLLTDSVPWPETGRPRRAGISSFGISGTNAHTIIEQ
                     AP"
     CDS_motif       complement(3897..3941)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43700_0003"
                     /evalue="1.80E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="329"
                     /protein_start="314"
                     /score="11.0"
                     /tool="antismash"
                     /translation="GSIKSNMGHTQAAAG"
     CDS_motif       complement(4002..4046)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43700_0002"
                     /evalue="4.30E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="294"
                     /protein_start="279"
                     /score="12.5"
                     /tool="antismash"
                     /translation="VEAHGTGTTLGDPIE"
     CDS_motif       complement(4422..4469)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43700_0001"
                     /evalue="7.90E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="CHF15_RS43700"
                     /protein_end="154"
                     /protein_start="138"
                     /score="20.9"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
ORIGIN
        1 agcgagtcga agcccagctc cttgaacgca cgatccgcag ggacctggtc gaccgaacca
       61 tgacccagaa cacccgccac ctcaccccgc accacatcga gcacggcctc ggcccgctcg
      121 tcctggggca gcgctgcgag ctgccgggcg agcttgcccg ccgcggccga accggccttg
      181 gcggcgcggc gagtgggaac gcggaccagt ccgctcagca gcggcggcag catcccgctg
      241 cccgcctggg cgcgcagcgc ggccaggtcg agcttcatgg gcacgaggac cggggcctgg
      301 gtggcgttgg ctgtgtcgaa gagggcgagg ccctcttcct ccgtcagggc gtccacgccg
      361 ccccgaccgg agcgcgccac ctcgccggcc atgccgccgt cccacaggcc ccaggccagc
      421 gacgtcgccg ccaagccctg cgacctgcgg tgcgccgcca gcgcgtccag gaaaacgttg
      481 gcggccgcgt agttcgcctg ccccgcgcca ccgaagacac cggcggccga ggagaacaga
      541 acgaacgccg acaggtccat gtcctgcgtg agctggtgga ggttccacgc ggcgtccacc
      601 ttcggatgca ggacccccga cagacgctcc tggtccaggg acgcgatgac accgtcgtcc
      661 aggacaccgg cggtgtgcac caccgccgtc agcggacgct ccgccggaat cgcgtccagc
      721 acagccgcca gggagtcccg gtcggccaca tcgcacgccg cccacgacac cgaagcaccc
      781 aggccgtcca gttcagcggt caggtccgca gcgcccggcg cggcctcgcc gcgacggctg
      841 accagcagca gatcccgcac gccgtactcg gacaccagat ggcgcgcgaa caggccgccc
      901 agcgcaccgc tggcacccgt caccagcacc gtgccctcaa gggcccgggc ttcggcgtcg
      961 ggattcgcga cggcccgtgc cagacgcggc gccaggaacg cgcccgcccg gagggcgatt
     1021 tcgggctcac cggtggccaa cgccgacgcc acggcctccg gggcgatctc accgtcgagg
     1081 tcgaccaggc cgaaacggcc cgggttctcc gactgcgccg accgcaccag gccccacacc
     1141 ggggcgtgca ccagatccgc gacctcgtcg cccggcacag cagccaccgc gccgcgcgtc
     1201 agaaacacca gacgcgactc ggcaaagcgc tcgtcgccca gccactcctg caccagcgcg
     1261 agcacccggc cggtctccgc atgcaccgca ccggccacgt ccggaaccgg ctcgtcggcc
     1321 acacacggca cgaccagcac cgccggaacc gcaccgctct cagcctcgac ggtctggagc
     1381 ccggcactcg ccagcttcca gtcgtcgacc gaaccaccgg ccggcaccgg cgaccactcg
     1441 acccggaaca gcgcgtcacc ctgaccggca cccggcccgg tgaactgctc cggcgcgaaa
     1501 gcccgcagca cgagaccggc cacggacgcc accgggacgc cctcggcgtc cgccagttcc
     1561 agcgccacca cgtcccggcc cgtacgcgac aaccgcaccc gcacctcgga cgcgccgacg
     1621 gcgtgcagcg tcacgccgga ccaggagaac ggcagcttcg cctcgccgtc ggtgccgctc
     1681 acgcccagtg cgtgcaacgc cgcgtccagc agcgccggat gcaccccgaa cgcacccgcc
     1741 tcagactcgg ccgactccgg cagccgcacc tcggcgaaca cgtcctcacc acgacgccac
     1801 accgaccgca gcccctggaa cgtcggcccg taaccgaacc ccgcctccac cagaccctcg
     1861 tacagaccgt caatcgcgac cggctccgcg ccgaccggcg gccacaccgc cagatcgaac
     1921 accggctccg ccgcccgccc ggccagcaca ccagcggcat gccgcaccca cgacccgtcc
     1981 tccactcgcg aatgcagcac cagcgcccga cggccctcac cgtcggcagc agccaccgac
     2041 aacctcagct gcacaccacc gtgctccggc agcaccagcg gcgcctccag cgtcagatcc
     2101 tccaggacct cacaacccac cagctcgccg gcgcgcaccg ccaactccac aaacgccgta
     2161 cccggcagca acaccgagcc catcaccgca tggtccgcga gccacggatg cgtcgtcagt
     2221 gacagacgac cggtgaacac caccccgtcc gaatccggca actcgaccgc cgcacccagc
     2281 aacggatgat ccgccgcacc gagaccgaat cctgtggcat cgccggggcc ggcctgtgcg
     2341 ccgcccgact cgagccagta ccactcgcgc tgaaaggcgt aggtgggcag ttcgacctgc
     2401 cggctgccgg tccgcgcgaa gtgcgcctcc cagtccacgg tgactccggc ggtgtaggcc
     2461 tgggccagag cagcggtcaa ggtgccgacc tcgggccggc cgccgcgcag ggccggggcg
     2521 aaggtggccc cttcctgagc caggcactcc tgcgccatcg ccgacagcac accgtccggg
     2581 ccgagttcga gatacgtggc gacgcccgcg gcctccaggg cccggacacc gtccaggaag
     2641 cgcaccgcct cacgaacgtg ccgcacccag aactcgggcg aggccatctc atcggtgacc
     2701 acggcaccgg tcaggttcga gacaaccgcg atacgcggct cctcgaacga cagccccgac
     2761 acgacctcac ggaaggcgtc cagcatcccg tccatgtgcg gcgagtggaa cgcgtgactg
     2821 acctggagcc gcttcgactt gcggtcaccg aaggactcca cgatcgcgac cgcggcgtcc
     2881 tcgtcgcccg cgatcaccac ggcctgcggc ccgttgacgg cagcgatgct cacgcgctcg
     2941 gtgagcagcg gcagaacctc gtcctccgat gcctgcaccg cgatcatcac accgcccgtg
     3001 ggcagcgcct gcatcaagcg gccacgagct tcgaccagcg tgcaggcgtc ctccagggac
     3061 agcaccccgg cgacgtgggc ggcggcgatc tcgccgatgg agtgaccgga cacgtagtcc
     3121 ggcttcacgc cccagctctc cagcagccgg aacagcgcca cctcgaccgc gaacaacgcc
     3181 ggctgcgcga actccgtccg gtccagcgcc tccgcgtcat cgccgaacag cacatcgagc
     3241 cccagccgct cacccaccgc gtccaaggcc ttggcgaaca ccggataggc ggcatacaac
     3301 tcacgcccca tccccagccg ctgactcccc tgccccgtga acaggaacgc ctgtttgccc
     3361 gtggtcgagg acgaaccccg caccaggccc gcggcaccgt gtccctcggc gagggcggac
     3421 aggccgcgca gcagctcgtc ccggtccgcc gccacgaccg acgcacgctg ctggaatgcg
     3481 gcgcgcctgg tggccagcga gtaggccaca tcggcggtgc cgatctccgg ggcctcttcg
     3541 aggaggtgcg tacgcaggcg ggccgcctgg tcccgtagtg cgtcctcggt cttcgccgac
     3601 agcacgtagg gcagtgcggt ggttgccggg gcggcggggg ccggccttgc cttcgtgatc
     3661 gcggcgaggt cgtcgggggc ctgctcgatg atcgtgtggg cgttggtgcc gctgatgccg
     3721 aaggacgaga tgccggcgcg acgcgggcgg ccggtctcgg gccacggaac cgaatccgtc
     3781 aggagggaga ccgcgccttc ggaccagtcg acgtgcgggg tcggctcgtc cacgtgcagc
     3841 gtcctgggca ggaccccgtg acggatcgcc atgatcatct tgatgatgcc cgccacacct
     3901 gccgcggcct gtgtgtggcc catgttcgac ttgatggagc cgatccagag cggctgctcg
     3961 tcgctgtgct cctggccgta ggtggccagc agtgcctgcg cctcgatcgg gtcacccagc
     4021 gtggtgccgg tgccgtgtgc ctcgaccgcg tcgatctcgt cgcccgacag gcccgcgctc
     4081 gcgagcgcgg cgcggatcac ccgctgctgg gacgggccgt tcggcgccgt caggccgttg
     4141 ctggcaccgt cctggttgat cgccgagccg cgcaccaccg ccagcaccgg gtggccgttg
     4201 cggcgcgcgt ccgacagccg ctcgacgagc agcatgcccg cgccctcggc ccaactggtt
     4261 ccgtccgccg cggccgcgaa ggacttgatc cggccgtcgg gggccagacc gcgctggcgg
     4321 ctgaactcag tgaaggtgcc cggagtcgcc atgaccgtca caccaccggc gagcgccatc
     4381 gtgcactcac cgttgcgcag cgcctggatc gcccagtgca gcgccaccag cgacgacgag
     4441 cacgcggtgt cgaccgtgac cgccggaccc tcgagcccga acgcgtacga cacacggccc
     4501 gacgcgatgc ttccggaatt tccggtaccg aggtatccct cgacgccgtc aggcacggag
     4561 gtgatccggg tggcgtagtc gtggtacatg atgcccgcga agacgccggt acggctgccg
     4621 cgcatcgcgg ccgggtcgat gcccgcccgc tcgaacgcct cccacgacgt ctccagcaac
     4681 agacgctgct gcgggtccat cgccaccgcc tcacgcggcg agatcccgaa gaacgcgggg
     4741 tcgaaccgac ccgcgtcgtg gaggaagcca cccgattgcg tgtagctcgt cccccggccg
     4801 tccgggtccg gatcgaacag cgcgtccaga tcccacccac gaccctgcgg gaacggcgag
     4861 atcgcatcgc caccaccgac cac
//
