LOCUS       NZ_FZOF01000073         4944 bp    DNA     linear   CON 02-JAN-2025
DEFINITION  Actinacidiphila glaucinigra strain CGMCC 4.1858, whole genome
            shotgun sequence.
ACCESSION   NZ_FZOF01000073
VERSION     NZ_FZOF01000073.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinacidiphila glaucinigra
  ORGANISM  Actinacidiphila glaucinigra
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Actinacidiphila.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2017) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900188405.1-RS_2025_01_02
            Annotation Date                   :: 01/02/2025 02:14:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,793
            CDSs (total)                      :: 8,707
            Genes (coding)                    :: 8,547
            CDSs (with protein)               :: 8,547
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 1 (5S, 16S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 47 of 160
            Pseudo Genes (incomplete)         :: 136 of 160
            Pseudo Genes (internal stop)      :: 6 of 160
            Pseudo Genes (multiple problems)  :: 29 of 160
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:25:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4944
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FZOF01000073.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4944
                     /db_xref="taxon:235986"
                     /mol_type="genomic DNA"
                     /note="contig: Ga0079895_173"
                     /organism="Actinacidiphila glaucinigra"
                     /strain="CGMCC 4.1858"
                     /type_material="type strain of Streptomyces glauciniger"
     gene            <1..>4944
                     /locus_tag="CHF15_RS43695"
                     /old_locus_tag="SAMN05216252_1731"
     region          1..4944
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..4944
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..4944
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[2:>4944](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3..>4944
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS             3..>4944
                     /NRPS_PKS="Domain: PKS_DH (3-169). E-value: 1.3e-65. Score:
                     212.8. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43695_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (497-675). E-value: 7.4e-56.
                     Score: 180.9. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43695_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (775-861). E-value: 1.9e-32.
                     Score: 103.4. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43695_PKS_PP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (891-1317). E-value:
                     3.9e-182. Score: 597.8. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43695_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (1421-1648). E-value: 1e-85.
                     Score: 279.6. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43695_PKS_AT.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009717830.1"
                     /locus_tag="CHF15_RS43695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216252_1731"
                     /product="type I polyketide synthase"
                     /protein_id="WP_143681804.1"
                     /sec_met_domain="adh_short (E-value: 2.2e-63, bitscore:
                     199.5, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 7.5e-15, bitscore:
                     41.5, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 2.9e-231, bitscore:
                     753.7, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 2.9e-94, bitscore:
                     301.4, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.7e-87, bitscore: 279.6,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGHPLLGATVALADSDGFLFTGRLSLDTHPWLADHAVMGTVLLP
                     GAAFVELAVRAGDQVGCDVLEELTLQAPLVLPERGGVQVQIHVAAPDADGTGRRTFTLS
                     SRAEDSAADVPWVRHADGVLAQGAGQPDFELTQWPPAGAEAVPTEDLYAELSALGMAYG
                     SAFRGLTAAWRRGESVYVEVALPEGHTAAARDFGLHPALLDAALHALALGGLGGNDGEG
                     RLPFSWTGVTLHAAGAEALRVHVARTGADDVRLEIADTTGAPVATVDALALRTVSAEQV
                     RAARTAYHDAVFRTEWTPLPGTPGTAPAQRRWAVVGGAAAIASLPGALTSEVGVHADLA
                     ALAAAVEAGAPVPDAVVVGYGPAEELSAQAVHAAAHDALALAQAWCADELFAGEAFAGC
                     RLVVVTQGAVTTGADTGITDPAHAAALGLLHSVQSEYPDRLLLVDTDGREDSARALPYA
                     LAADEPRLAVRAGASHAPRLVRVARQDDDADVTSYAPDKTVLITGAGGMLGGLVARRLV
                     TGHGVRHLLLVGRRGGTTPDAERLRAELTAAGATVTWAACDVADREALADVLRGIPDAH
                     PLGAIVHTAGALDDGVLTSLTPERLSAVLRPKADAALNLHDLTRDLDLSAFVLFSSIGG
                     VFGGAGQGNYAAANVFLDALAHHRRSQGLAATSMAWALWAGGGDATGGPGMAGGLDEAD
                     ISRMRRAGLPPLTTAEGLELFDLAHRVGEAAPVLMRADTAALRTQAQTGSLSPLLRGLV
                     GAPTRRAVAATGAEGEAGFRERLAGLAPAERDRALLDLVRKQVATALGYPGPASVEPGR
                     SFKELGFDSLTAVELRNLLGAATGKRLPATLVFDYPTATALAGYLREELVGDLTDGDAA
                     RAGTALAPTGPRTVHADDDPIAIVAMSCRFPGGVTSPEDLWQLLAAGRDGITGFPADRG
                     WDLDHLYSDDPDSEGTSYAREGGFLHDAADFDADFFGVSPREALAMDPQQRLLLETTWE
                     TFERAGIDPTTLRGSRTGVFVGSNAQDYLHLWLEDADGLEGHLGTGNAASVVSGRIAYT
                     FGLEGPAVTIDTACSSSLVTLHLAAQALRRGDCSMALAGAVTIMSTPGAFTEFSRQRGL
                     AADGRIKAFAASADGTSWSEGVALLLVERLSDARANGHPVLAVVRGSAVNQDGASNGLT
                     APNGPSQQRVIREALADAGLSATDVDAVEAHGTGTTLGDPIEAQALLATYGQGRSADRP
                     VWLGSVKSNIGHTQAVAGAAGVIKMVMAMRHGLLPRTLHVDEPTPYVDWSAGDISLLTE
                     AVEWPDTGRPRRAGVSSFGYSGTNAHAIIEQAPDSVVPAPEGPARDLPVVPWPVSARTA
                     TALQAQAERLHQAAAGSEATRGIDLAYSLATSRAALEHRAVLVAPDPAAQLDALAALAR
                     GEHAPGLVRGAVSTGALAFLFTGQGSQRLGMGRELYAAYPVFAEALDAVGERLGLGVLF
                     GDDAEALDRTEFAQPALFAVEVALFRLLESWGVRPDFVSGHSVGEIAAAHVAGVLSLED
                     ACTLVAARGRLMQALPTAGVMIALQASEDEVLPLLNDRVSVAAVNGPESVVIAGDEDAA
                     VAIVEAFADRKSKRLTVSHAFHSPHMDGMLADFREVVAGLSFEAPRIPVVSNLTGAVVA
                     DDMGSPEFWV"
     aSDomain        13..510
                     /ASF="catalytic triad H,G,P found: False"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43695_PKS_DH.1"
                     /evalue="1.30E-65"
                     /label="CHF15_RS43695_PKS_DH.1"
                     /locus_tag="CHF15_RS43695"
                     /protein_end="169"
                     /protein_start="3"
                     /score="212.8"
                     /tool="antismash"
                     /translation="HPLLGATVALADSDGFLFTGRLSLDTHPWLADHAVMGTVLLPGAA
                     FVELAVRAGDQVGCDVLEELTLQAPLVLPERGGVQVQIHVAAPDADGTGRRTFTLSSRA
                     EDSAADVPWVRHADGVLAQGAGQPDFELTQWPPAGAEAVPTEDLYAELSALGMAYGSAF
                     RGL"
     aSModule        13..2586
                     /domains="nrpspksdomains_CHF15_RS43695_PKS_DH.1"
                     /domains="nrpspksdomains_CHF15_RS43695_PKS_KR.1"
                     /domains="nrpspksdomains_CHF15_RS43695_PKS_PP.1"
                     /incomplete
                     /locus_tags="CHF15_RS43695"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       97..171
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43695_0001"
                     /evalue="8.90E-10"
                     /label="PKSI-DH_m2"
                     /locus_tag="CHF15_RS43695"
                     /protein_end="56"
                     /protein_start="31"
                     /score="31.0"
                     /tool="antismash"
                     /translation="WLADHAVMGTVLLPGAAFVELAVRA"
     aSDomain        1495..2028
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43695_PKS_KR.1"
                     /evalue="7.40E-56"
                     /label="CHF15_RS43695_PKS_KR.1"
                     /locus_tag="CHF15_RS43695"
                     /protein_end="675"
                     /protein_start="497"
                     /score="180.9"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="TVLITGAGGMLGGLVARRLVTGHGVRHLLLVGRRGGTTPDAERLR
                     AELTAAGATVTWAACDVADREALADVLRGIPDAHPLGAIVHTAGALDDGVLTSLTPERL
                     SAVLRPKADAALNLHDLTRDLDLSAFVLFSSIGGVFGGAGQGNYAAANVFLDALAHHRR
                     SQGLAATSMAWALWA"
     CDS_motif       1879..1983
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43695_0002"
                     /evalue="9.50E-21"
                     /label="PKSI-KR_m4"
                     /locus_tag="CHF15_RS43695"
                     /protein_end="660"
                     /protein_start="625"
                     /score="66.4"
                     /tool="antismash"
                     /translation="AFVLFSSIGGVFGGAGQGNYAAANVFLDALAHHRR"
     aSDomain        2329..2586
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43695_PKS_PP.1"
                     /evalue="1.90E-32"
                     /label="CHF15_RS43695_PKS_PP.1"
                     /locus_tag="CHF15_RS43695"
                     /protein_end="861"
                     /protein_start="775"
                     /score="103.4"
                     /tool="antismash"
                     /translation="LAGLAPAERDRALLDLVRKQVATALGYPGPASVEPGRSFKELGFD
                     SLTAVELRNLLGAATGKRLPATLVFDYPTATALAGYLREEL"
     aSDomain        2677..3954
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43695_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="3.90E-182"
                     /label="CHF15_RS43695_PKS_KS.1"
                     /locus_tag="CHF15_RS43695"
                     /protein_end="1317"
                     /protein_start="891"
                     /score="597.8"
                     /tool="antismash"
                     /translation="IAIVAMSCRFPGGVTSPEDLWQLLAAGRDGITGFPADRGWDLDHL
                     YSDDPDSEGTSYAREGGFLHDAADFDADFFGVSPREALAMDPQQRLLLETTWETFERAG
                     IDPTTLRGSRTGVFVGSNAQDYLHLWLEDADGLEGHLGTGNAASVVSGRIAYTFGLEGP
                     AVTIDTACSSSLVTLHLAAQALRRGDCSMALAGAVTIMSTPGAFTEFSRQRGLAADGRI
                     KAFAASADGTSWSEGVALLLVERLSDARANGHPVLAVVRGSAVNQDGASNGLTAPNGPS
                     QQRVIREALADAGLSATDVDAVEAHGTGTTLGDPIEAQALLATYGQGRSADRPVWLGSV
                     KSNIGHTQAVAGAAGVIKMVMAMRHGLLPRTLHVDEPTPYVDWSAGDISLLTEAVEWPD
                     TGRPRRAGVSSFGYSGTNAHAIIEQAP"
     aSModule        2677..>4944
                     /domains="nrpspksdomains_CHF15_RS43695_PKS_KS.1"
                     /domains="nrpspksdomains_CHF15_RS43695_PKS_AT.1"
                     /incomplete
                     /locus_tags="CHF15_RS43695"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       3160..3204
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43695_0003"
                     /evalue="3.40E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="CHF15_RS43695"
                     /protein_end="1067"
                     /protein_start="1052"
                     /score="19.0"
                     /tool="antismash"
                     /translation="GPAVTIDTACSSSLV"
     misc_feature    3294..3296
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     CDS_motif       3583..3627
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43695_0004"
                     /evalue="4.40E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="CHF15_RS43695"
                     /protein_end="1208"
                     /protein_start="1193"
                     /score="12.5"
                     /tool="antismash"
                     /translation="VEAHGTGTTLGDPIE"
     CDS_motif       3688..3732
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43695_0005"
                     /evalue="2.60E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="CHF15_RS43695"
                     /protein_end="1243"
                     /protein_start="1228"
                     /score="10.5"
                     /tool="antismash"
                     /translation="GSVKSNIGHTQAVAG"
     aSDomain        4267..>4944
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43695_PKS_AT.1"
                     /evalue="1.00E-85"
                     /label="CHF15_RS43695_PKS_AT.1"
                     /locus_tag="CHF15_RS43695"
                     /protein_end="1648"
                     /protein_start="1421"
                     /score="279.6"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFTGQGSQRLGMGRELYAAYPVFAEALDAVGERLGLGVLFGDDAE
                     ALDRTEFAQPALFAVEVALFRLLESWGVRPDFVSGHSVGEIAAAHVAGVLSLEDACTLV
                     AARGRLMQALPTAGVMIALQASEDEVLPLLNDRVSVAAVNGPESVVIAGDEDAAVAIVE
                     AFADRKSKRLTVSHAFHSPHMDGMLADFREVVAGLSFEAPRIPVVSNLTGAVVADDMGS
                     PEFWV"
     CDS_motif       4270..4317
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43695_0006"
                     /evalue="8.10E-06"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="CHF15_RS43695"
                     /protein_end="1438"
                     /protein_start="1422"
                     /score="18.3"
                     /tool="antismash"
                     /translation="FTGQGSQRLGMGRELY"
     CDS_motif       4405..4470
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43695_0007"
                     /evalue="7.10E-05"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="CHF15_RS43695"
                     /protein_end="1489"
                     /protein_start="1467"
                     /score="15.9"
                     /tool="antismash"
                     /translation="LDRTEFAQPALFAVEVALFRLL"
     CDS_motif       4480..4602
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43695_0008"
                     /evalue="4.70E-24"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="CHF15_RS43695"
                     /protein_end="1533"
                     /protein_start="1492"
                     /score="76.7"
                     /tool="antismash"
                     /translation="GVRPDFVSGHSVGEIAAAHVAGVLSLEDACTLVAARGRLMQ"
     CDS_motif       4687..4725
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43695_0009"
                     /evalue="3.30E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="CHF15_RS43695"
                     /protein_end="1574"
                     /protein_start="1561"
                     /score="13.9"
                     /tool="antismash"
                     /translation="AAVNGPESVVIAG"
ORIGIN
        1 ggccctgggt cacccgctgc tgggcgcgac cgtggcgctc gccgactccg acggcttcct
       61 cttcacgggc cgtctctccc tcgacacgca cccctggctc gccgaccacg ccgtcatggg
      121 caccgtcctg cttcccggcg cggcctttgt ggagttggcc gttcgtgccg gtgaccaggt
      181 cggttgtgac gtgctggagg aactcaccct ccaggccccg ctcgttctcc ccgagcgcgg
      241 cggcgtgcag gtgcagatcc acgtggccgc gcccgacgcg gacggcacag gtcgtcgcac
      301 gttcacgctc tcctcccgcg ccgaggacag cgccgccgat gtgccgtggg tgcggcacgc
      361 cgacggcgtc ctcgcccaag gcgccggaca gccggacttc gagctcaccc agtggccccc
      421 ggcgggagcc gaggccgtcc cgacggagga cctctacgcg gagctctctg cgctcggcat
      481 ggcctacggc tccgccttcc gtggactgac cgccgcgtgg cggcgcgggg agagcgtcta
      541 cgtcgaggtc gcgctgcccg aggggcacac cgctgccgca cgggacttcg gcctgcaccc
      601 cgccctgctc gacgcggccc tgcacgcgct cgccctcggc gggctgggcg gaaacgacgg
      661 cgagggacgg ctgccgttct cctggaccgg ggtgacgctg cacgccgcgg gcgccgaggc
      721 cctgcgcgtc cacgtcgcgc gaacgggggc ggacgacgtg cgcctggaga tcgccgacac
      781 caccggcgca cccgtcgcca ccgtggacgc cctcgcactg cgcacggtct ccgccgagca
      841 ggtacgggcc gcgcggaccg cgtaccacga cgcggtgttc cgcacggagt ggacgcccct
      901 gccgggcacc cccggcaccg cccccgcaca gcggcgttgg gccgtcgtcg gcggggccgc
      961 cgcaatcgcg tcactgccgg gagcgctgac cagcgaggtc ggcgtccacg ccgacctggc
     1021 cgcactcgcg gcggccgtcg aggccggggc gcccgtgccg gacgccgtgg tcgtcgggta
     1081 cggcccggcg gaggagctgt cggcccaggc cgtgcacgcc gccgcgcacg acgccctcgc
     1141 cctcgcccag gcgtggtgcg ccgacgagct cttcgcgggt gaggccttcg ccggctgccg
     1201 gctggtcgtc gtcacccagg gcgcggtcac caccggcgcc gacaccggga tcaccgaccc
     1261 cgcgcacgcc gccgcactgg gcctgctgca ctccgtgcag tccgagtacc ccgaccggct
     1321 gctgctcgtc gacaccgacg gacgcgagga ctccgcccgc gccctgccgt acgccctcgc
     1381 cgcggacgag ccccgactcg ccgtgcgcgc cggcgcttcg cacgcccccc ggctcgtccg
     1441 cgtcgcccgg caggacgacg acgccgacgt cacctcctac gcgcccgaca agaccgtact
     1501 gatcaccggg gcgggcggca tgctcggcgg tctcgtcgcc cgccgcctcg tcaccgggca
     1561 cggcgtacgc cacctgctgc tcgtgggacg ccggggcggc acgaccccgg acgccgagcg
     1621 actgcgcgcc gaactgaccg cggcgggcgc cacggtgacg tgggcggcct gcgacgtcgc
     1681 cgaccgcgaa gccctcgccg acgtcctgcg cggcatcccg gacgcgcacc cgctcggcgc
     1741 gatcgtccac accgcgggcg cgctcgacga cggcgtcctc acgtccctca ccccggaacg
     1801 gctctccgcg gtcctgcgcc ccaaggccga cgcggcgctc aacctgcacg acctcacccg
     1861 ggacctcgac ctctcggcct tcgtcctctt ctcctccatc ggcggcgtct tcggcggcgc
     1921 cgggcagggc aactacgcgg ccgccaacgt cttcctggac gccctcgccc accaccggcg
     1981 gtcccagggc ctcgccgcca cctccatggc gtgggccctg tgggccggcg gaggggacgc
     2041 gacgggcggc ccgggcatgg ccggcggcct cgacgaggcc gacatcagcc gcatgcggcg
     2101 cgcgggcctg cccccgctga ccacggcgga aggcctggag ctcttcgacc tcgcccaccg
     2161 cgtcggtgag gcggcacccg tcctgatgcg tgcggacacc gcggcgctgc gcacccaggc
     2221 ccagaccggc tccctgtcac cgctgctgcg tggtctggtc ggggctccca ctcgccgtgc
     2281 cgtggccgcc acgggcgcgg aaggagaggc cgggttccgg gagcgtctcg ccgggctcgc
     2341 ccccgccgaa cgtgaccgcg ccctgctgga tctggtccgc aagcaggtcg ccaccgcgct
     2401 cggctacccc ggaccggcct ccgtcgagcc cgggcgctcg ttcaaggagc tgggcttcga
     2461 ctccctgacc gctgtcgagc tgcgcaacct gctcggtgcc gcaaccggca agcgcctgcc
     2521 cgccacgctc gtcttcgact acccgacggc caccgcgctc gccggatacc tgcgcgaaga
     2581 actcgtcggc gacctgacgg acggggacgc ggcccgggcg gggaccgccc tcgcgccgac
     2641 cggaccgcga acggtccacg cggacgacga ccccatcgcg atcgtcgcga tgagctgccg
     2701 cttccccggc ggcgtcacgt cccccgaaga cctctggcag ctcctggccg ccggacgcga
     2761 cggcatcacc ggcttccccg ccgaccgcgg ctgggacctc gaccacctct acagcgacga
     2821 ccccgacagc gagggcacca gctacgcccg cgagggcggc ttcctccacg acgccgccga
     2881 cttcgacgcc gacttcttcg gcgtctcgcc gcgtgaggcg ctggccatgg acccgcagca
     2941 gcgcctgctg ctggagacca cctgggagac gttcgaacgc gccgggatcg accccaccac
     3001 gctgcggggc agccgcaccg gcgtgttcgt cggctccaac gcccaggact acctgcacct
     3061 gtggctcgaa gacgccgacg gcctcgaagg ccacctgggc accggcaatg cggccagcgt
     3121 cgtctccggc cgcatcgcct acaccttcgg gctcgagggc ccggccgtca ccatcgacac
     3181 cgcctgctcg tcgtccctgg tcaccctgca cctggcagcg caggcgctgc gccgcggcga
     3241 ctgctcgatg gctctcgccg gcgccgtcac catcatgtcc acccctggcg cgtttaccga
     3301 gttcagccgc cagcgcggcc tggccgcgga cggccgcatc aaggcgttcg ccgcgtcggc
     3361 ggacgggacg agctggtccg agggcgtcgc gctgcttctt gtcgagcgac tctcggacgc
     3421 ccgagccaac ggccacccgg tgctggcggt cgtgcgcggt tctgccgtca accaggacgg
     3481 cgcgagcaac ggcctgaccg ccccgaacgg cccctcccag cagcgtgtca tccgggaagc
     3541 cctcgccgac gccggcctgt cggccacgga cgtcgacgcg gtcgaggccc acggcacggg
     3601 caccaccctg ggcgacccca tcgaggcgca ggcactcctc gccacctacg gccagggccg
     3661 atccgccgac cgcccggtgt ggctcggctc cgtcaagtcg aacatcggac acacccaggc
     3721 cgtcgccggt gccgccggtg tcatcaagat ggtcatggcg atgcggcacg gcctgctgcc
     3781 gcggaccctg cacgtggacg agccgacccc gtacgtcgac tggtcggcgg gagacatctc
     3841 cctgctgacc gaggccgtcg agtggcccga caccggccgc ccgcgccgcg ccggcgtctc
     3901 ctccttcggc tacagcggca ccaacgccca cgcgatcatc gagcaggccc ccgacagcgt
     3961 cgtgcccgcc ccggaggggc cggcccgcga cctgcccgtc gtaccctggc cggtctccgc
     4021 ccgcacggcg accgcactgc aggcccaggc ggagcgcctg caccaggctg ccgccggcag
     4081 cgaggccacg cgtggcatcg acctcgccta ctccctcgcg accagccgcg ccgccctgga
     4141 acaccgggcg gttctcgtcg caccggaccc ggccgcccag ctcgacgccc tcgccgccct
     4201 ggcccgcgga gagcacgcac ccggcctggt gcggggtgcc gtgagcaccg gcgcgctcgc
     4261 cttcctgttc acggggcagg ggagtcagcg actggggatg gggcgggagt tgtatgccgc
     4321 ctatccggtg ttcgccgagg cgttggacgc tgtgggcgag cggcttgggc tgggtgtgct
     4381 gttcggtgat gacgcggagg cgttggatcg cacggagttc gcgcagccgg cgttgttcgc
     4441 ggtggaggtg gcgctgttcc ggctgctgga gagctggggc gtgcggccgg acttcgtgtc
     4501 gggtcactcc gtgggtgaga tcgcggccgc gcatgtggcg ggggtgttgt ccctggagga
     4561 tgcgtgcacg ctggttgcgg cgcgtggtcg gttgatgcag gcgctgccga cggctggcgt
     4621 gatgatcgcg ctgcaggcgt cggaggacga ggttctgccg ctgctgaatg atcgcgtgag
     4681 cgtcgcggct gtgaacggtc cggagtcggt cgtcatcgcg ggtgacgagg atgccgcggt
     4741 cgcgatcgtg gaggccttcg cggaccggaa gtcaaagcgg ctgacggtca gtcacgcttt
     4801 ccactcgccg cacatggacg ggatgctggc ggacttccgt gaggtggtgg cggggctgtc
     4861 gttcgaggcc ccgcgtatcc cggtcgtgtc gaacctgacg ggcgccgtcg tcgcagatga
     4921 catgggctcg cccgagttct gggt
//
