LOCUS       NZ_FZOF01000071         6149 bp    DNA     linear   CON 02-JAN-2025
DEFINITION  Actinacidiphila glaucinigra strain CGMCC 4.1858, whole genome
            shotgun sequence.
ACCESSION   NZ_FZOF01000071
VERSION     NZ_FZOF01000071.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinacidiphila glaucinigra
  ORGANISM  Actinacidiphila glaucinigra
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Actinacidiphila.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2017) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900188405.1-RS_2025_01_02
            Annotation Date                   :: 01/02/2025 02:14:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,793
            CDSs (total)                      :: 8,707
            Genes (coding)                    :: 8,547
            CDSs (with protein)               :: 8,547
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 1 (5S, 16S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 47 of 160
            Pseudo Genes (incomplete)         :: 136 of 160
            Pseudo Genes (internal stop)      :: 6 of 160
            Pseudo Genes (multiple problems)  :: 29 of 160
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:25:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6149
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FZOF01000071.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     cand_cluster    1..6149
                     /SMILES="CC(O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6149
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[3126:>6149](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3127..>6149
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     gene            <1..3102
                     /locus_tag="CHF15_RS43680"
                     /old_locus_tag="SAMN05216252_1711"
     CDS             <1..3102
                     /NRPS_PKS="Domain: PKS_AT (0-221). E-value: 4e-81. Score:
                     264.6. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43680_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_KR (566-746). E-value: 3e-56. Score:
                     182.2. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43680_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (854-926). E-value: 1.1e-31. Score:
                     100.9. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43680_ACP.1"
                     /NRPS_PKS="Domain: PKS_Docking_Cterm (947-1016). E-value:
                     5.2e-13. Score: 41.6. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43680_PKS_Docking_Cterm.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060790.1"
                     /locus_tag="CHF15_RS43680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216252_1711"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_143681801.1"
                     /sec_met_domain="PKS_AT (E-value: 1.3e-82, bitscore: 264.6,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 2.5e-60, bitscore:
                     190.5, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 3.1e-08,
                     bitscore: 20.1, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2e-15, bitscore:
                     44.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VEVALFRLLESWGVRPDFVSGHSLGEIAAAHVAGVFSLEDACTLV
                     AARGRLMQALPAGGVMIALQASEGEVLPLLNDRVSIAAVNGPESVVIAGDEDAALAIVG
                     FFAGRKSKRLTVSHAFHSPDMDGMLADFRKVAEGLAYAAPRIPLVSNLTGALVTDEVAS
                     ADFWVRHVREAVRFLDGVRALEAVGVTTYLELGPDGVLSAMAQDCVTGDGAAFVPVLRG
                     GRPEAGTAVTALAQAHVRGATVEWARFFAGTGARRVDLPTYAFQGRRYWPEPAAPLPAA
                     DAARDEADARFWEVVESADPNGIADEFGIDADQPLSAVLPALSLWRRDRQARTQVDARR
                     YRVTWHSWPNGGGNAGATPSGVWLAALPAAHADDPWISAVTARVEAGGARVVPVFLDTT
                     DAEQDVLAARLREHLAAAVASGPVAGVLSLLALDERPHPEHPGVPVGLALTAALAEVLT
                     DDASGVDGALWCVTRDAVAAVPADGITGAAQAQVWGLGRVVALEHPDRWGGLVDLPADC
                     DDRVLHRLAAVLAAVGDEDQLAVRASGTFVRRLERAAPQGPGAATWTPRGTVLVTGGTG
                     ALGRHVARWLAERGAEHLVLVSRKGADAPGAARIEEELSGLGAEVTLAACDVADRAALE
                     ALVARLAAEGTPVRAVVHAAGVSQPPGTHADLRGLAHVVAAKTAGAVHLDALFDTPDSL
                     DAFVLFSSIAGVWGSGGQGAYAAANAFLDALAERRGARGLPATALAWGPWADGGMAAEG
                     DAEEQLRRRGLPPMAPADNLLALERAVTGPDAALTVAEVDWARFAPAYAAARPRPFVAD
                     LPEVRDALRADPPAVGGGTDTPSSATLRRLAELTGDDRRVALLDLVREHAAVALGHTVA
                     DAVAAERAFKDLGFDSLTAVELRNRLGAACGLRLPSSLVFDHPNPQALARYLLHSLFPD
                     TTGAAGAASAHPVDTDPQDAELRRTLATIPLGRIREAGLLDTLLRLAASDGTPAGAADP
                     ADPSEAADSIDAMDLQDLLDLALDGHGDPDGSHGSATDDDSNRS"
     source          1..6149
                     /db_xref="taxon:235986"
                     /mol_type="genomic DNA"
                     /note="contig: Ga0079895_171"
                     /organism="Actinacidiphila glaucinigra"
                     /strain="CGMCC 4.1858"
                     /type_material="type strain of Streptomyces glauciniger"
     region          1..6149
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     aSDomain        4..666
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43680_PKS_AT.1"
                     /evalue="4.00E-81"
                     /label="CHF15_RS43680_PKS_AT.1"
                     /locus_tag="CHF15_RS43680"
                     /protein_end="221"
                     /protein_start="0"
                     /score="264.6"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="VEVALFRLLESWGVRPDFVSGHSLGEIAAAHVAGVFSLEDACTLV
                     AARGRLMQALPAGGVMIALQASEGEVLPLLNDRVSIAAVNGPESVVIAGDEDAALAIVG
                     FFAGRKSKRLTVSHAFHSPDMDGMLADFRKVAEGLAYAAPRIPLVSNLTGALVTDEVAS
                     ADFWVRHVREAVRFLDGVRALEAVGVTTYLELGPDGVLSAMAQDCVTGDGAAFVPVLR"
     aSModule        4..2781
                     /complete
                     /domains="nrpspksdomains_CHF15_RS43680_PKS_AT.1"
                     /domains="nrpspksdomains_CHF15_RS43680_PKS_KR.1"
                     /domains="nrpspksdomains_CHF15_RS43680_ACP.1"
                     /locus_tags="CHF15_RS43680"
                     /monomer_pairings="mal -> ohmal"
                     /starter_module
                     /tool="antismash"
                     /type="pks"
     CDS_motif       40..162
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43680_0001"
                     /evalue="1.60E-24"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="CHF15_RS43680"
                     /protein_end="53"
                     /protein_start="12"
                     /score="78.3"
                     /tool="antismash"
                     /translation="GVRPDFVSGHSLGEIAAAHVAGVFSLEDACTLVAARGRLMQ"
     CDS_motif       247..285
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43680_0002"
                     /evalue="1.90E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="CHF15_RS43680"
                     /protein_end="94"
                     /protein_start="81"
                     /score="14.6"
                     /tool="antismash"
                     /translation="AAVNGPESVVIAG"
     CDS_motif       433..465
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43680_0003"
                     /evalue="2.00E-03"
                     /label="PKSI-AT-M_m7"
                     /locus_tag="CHF15_RS43680"
                     /protein_end="154"
                     /protein_start="143"
                     /score="10.9"
                     /tool="antismash"
                     /translation="PRIPLVSNLTG"
     CDS_motif       502..534
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43680_0004"
                     /evalue="7.50E-04"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="CHF15_RS43680"
                     /protein_end="177"
                     /protein_start="166"
                     /score="13.0"
                     /tool="antismash"
                     /translation="WVRHVREAVRF"
     aSDomain        1702..2241
                     /ASF="KR domain putatively catalyzing L-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43680_PKS_KR.1"
                     /evalue="3.00E-56"
                     /label="CHF15_RS43680_PKS_KR.1"
                     /locus_tag="CHF15_RS43680"
                     /protein_end="746"
                     /protein_start="566"
                     /score="182.2"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: A1"
                     /tool="antismash"
                     /translation="GTVLVTGGTGALGRHVARWLAERGAEHLVLVSRKGADAPGAARIE
                     EELSGLGAEVTLAACDVADRAALEALVARLAAEGTPVRAVVHAAGVSQPPGTHADLRGL
                     AHVVAAKTAGAVHLDALFDTPDSLDAFVLFSSIAGVWGSGGQGAYAAANAFLDALAERR
                     GARGLPATALAWGPWAD"
     CDS_motif       1705..1800
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43680_0005"
                     /evalue="3.50E-17"
                     /label="PKSI-KR_m1"
                     /locus_tag="CHF15_RS43680"
                     /protein_end="599"
                     /protein_start="567"
                     /score="54.9"
                     /tool="antismash"
                     /translation="TVLVTGGTGALGRHVARWLAERGAEHLVLVSR"
     CDS_motif       2083..2190
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43680_0006"
                     /evalue="1.40E-20"
                     /label="PKSI-KR_m4"
                     /locus_tag="CHF15_RS43680"
                     /protein_end="729"
                     /protein_start="693"
                     /score="65.8"
                     /tool="antismash"
                     /translation="LDAFVLFSSIAGVWGSGGQGAYAAANAFLDALAERR"
     aSDomain        2566..2781
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43680_ACP.1"
                     /evalue="1.10E-31"
                     /label="CHF15_RS43680_ACP.1"
                     /locus_tag="CHF15_RS43680"
                     /protein_end="926"
                     /protein_start="854"
                     /score="100.9"
                     /tool="antismash"
                     /translation="LDLVREHAAVALGHTVADAVAAERAFKDLGFDSLTAVELRNRLGA
                     ACGLRLPSSLVFDHPNPQALARYLLHS"
     aSDomain        2845..3051
                     /aSDomain="PKS_Docking_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43680_PKS_Docking_Cterm.
                     1"
                     /evalue="5.20E-13"
                     /label="CHF15_RS43680_PKS_Docking_Cterm.1"
                     /locus_tag="CHF15_RS43680"
                     /protein_end="1016"
                     /protein_start="947"
                     /score="41.6"
                     /tool="antismash"
                     /translation="QDAELRRTLATIPLGRIREAGLLDTLLRLAASDGTPAGAADPADP
                     SEAADSIDAMDLQDLLDLALDGHG"
     gene            3127..>6149
                     /locus_tag="CHF15_RS43685"
                     /old_locus_tag="SAMN05216252_1712"
     CDS             3127..>6149
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (36-462). E-value:
                     7.5e-182. Score: 596.9. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43685_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (564-845). E-value: 6.9e-104.
                     Score: 339.3. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43685_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (913-1006). E-value: 7.5e-43.
                     Score: 138.7. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43685_PKS_DH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742156.1"
                     /locus_tag="CHF15_RS43685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216252_1712"
                     /product="type I polyketide synthase"
                     /protein_id="WP_143681802.1"
                     /sec_met_domain="mod_KS (E-value: 7.1e-233, bitscore:
                     760.0, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 2e-93, bitscore:
                     299.7, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 2.3e-105, bitscore:
                     339.3, seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPNEKVVEALRASLKETERLRRRNRELTDASREPIAIVAMGCR
                     FPGGVRSPEDLWKLVDSGGDGITGFPVDRGWDIESLYDPDPDHEGTTYARHGGFLHDAG
                     DFDPSFFGISPREALAMDPQQRLLLETTWETFERAGIDPATLRGSRAGVFVGASANAYG
                     AGAGDLPDGVEGHLLTGTASSVVSGRLAYVFGLEGPAATIDTACSSSSVALHMAVQALR
                     QGECSLALAAGVTVLAGPDVFVEFSRQRGLSPDGRCRSFAEAADGTGWSEGVGVLLVER
                     LSDARRNGHPVLAVVRGSAVNQDGASNGLTAPNGPSQQRVIRQALENARLTPADIDAVE
                     AHGTGTTLGDPIEAQALLATYGQDRPADRPLLLGSLKSNIGHTQNAAGVAGIIKMVMAM
                     RHGVLPQTLHVDEPTSHVDWSSGAVSLLTEAVRWPETGRPRRFGVSAFGVSGTNVHTIL
                     EEAPEPEATAPAPAVGGVVPWLLSGKGHEALREQARRLHAYVGENPGSRAVDVAHSLAV
                     GRASFDDRAVVVAEDRAGLLSGLAALSEGEVTPGVLTGSPVAGKVAFLFTGQGSQRLGM
                     GRELYAAYPVFARALDEVRERLGLDVLFGDDAEALDRTESAQPALFAIEVALFRLLESW
                     GVRPDFLSGHSVGEIAAAHVAGVLSLEDACTLVAARGRLMQALPMGGVMIALQASEDEV
                     LPLLNGRVSIAAVNGPESVVVAGDEDAAVAIAESFPDRKSKRLTVSHAFHSPHMDGMLD
                     AFREVVAGLSFAAPRIPVVSNLTGALVTDEMASPEFWVRHVREAVRFLDGVRALEAAGV
                     TTFIELGPDGVLSAMAQECVSGEGAAFLPVLRKGRSEAETVVTAVAQAHAWGIAVDWPA
                     FFAGTGARRVDLPTYAFQRQRYWIESGRGAGDLRAVGLEALGHPLLGATVALADSDGFL
                     FTGRLSLDTHPWLADHAVMGTVLLPGAAFVELAVRAGDQVGCDVLEELTLQAPLVLPER
                     GGVQVQIHVAAPDADGTGR"
     misc_feature    complement(3140..3155)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     aSDomain        3235..4512
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43685_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="7.50E-182"
                     /label="CHF15_RS43685_PKS_KS.1"
                     /locus_tag="CHF15_RS43685"
                     /protein_end="462"
                     /protein_start="36"
                     /score="596.9"
                     /tool="antismash"
                     /translation="IAIVAMGCRFPGGVRSPEDLWKLVDSGGDGITGFPVDRGWDIESL
                     YDPDPDHEGTTYARHGGFLHDAGDFDPSFFGISPREALAMDPQQRLLLETTWETFERAG
                     IDPATLRGSRAGVFVGASANAYGAGAGDLPDGVEGHLLTGTASSVVSGRLAYVFGLEGP
                     AATIDTACSSSSVALHMAVQALRQGECSLALAAGVTVLAGPDVFVEFSRQRGLSPDGRC
                     RSFAEAADGTGWSEGVGVLLVERLSDARRNGHPVLAVVRGSAVNQDGASNGLTAPNGPS
                     QQRVIRQALENARLTPADIDAVEAHGTGTTLGDPIEAQALLATYGQDRPADRPLLLGSL
                     KSNIGHTQNAAGVAGIIKMVMAMRHGVLPQTLHVDEPTSHVDWSSGAVSLLTEAVRWPE
                     TGRPRRFGVSAFGVSGTNVHTILEEAP"
     aSModule        3235..6144
                     /domains="nrpspksdomains_CHF15_RS43685_PKS_KS.1"
                     /domains="nrpspksdomains_CHF15_RS43685_PKS_AT.1"
                     /domains="nrpspksdomains_CHF15_RS43685_PKS_DH.1"
                     /incomplete
                     /locus_tags="CHF15_RS43685"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       3634..3696
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43685_0001"
                     /evalue="4.50E+00"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="CHF15_RS43685"
                     /protein_end="190"
                     /protein_start="169"
                     /score="0.6"
                     /tool="antismash"
                     /translation="PDGVEGHLLTGTASSVVSGRL"
     CDS_motif       3718..3765
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43685_0002"
                     /evalue="3.90E-05"
                     /label="PKSI-KS_m3"
                     /locus_tag="CHF15_RS43685"
                     /protein_end="213"
                     /protein_start="197"
                     /score="15.9"
                     /tool="antismash"
                     /translation="GPAATIDTACSSSSVA"
     CDS_motif       4141..4185
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43685_0003"
                     /evalue="2.30E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="CHF15_RS43685"
                     /protein_end="353"
                     /protein_start="338"
                     /score="13.2"
                     /tool="antismash"
                     /translation="VEAHGTGTTLGDPIE"
     CDS_motif       4246..4290
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43685_0004"
                     /evalue="2.20E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="CHF15_RS43685"
                     /protein_end="388"
                     /protein_start="373"
                     /score="10.7"
                     /tool="antismash"
                     /translation="GSLKSNIGHTQNAAG"
     aSDomain        4819..5661
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43685_PKS_AT.1"
                     /evalue="6.90E-104"
                     /label="CHF15_RS43685_PKS_AT.1"
                     /locus_tag="CHF15_RS43685"
                     /protein_end="845"
                     /protein_start="564"
                     /score="339.3"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFTGQGSQRLGMGRELYAAYPVFARALDEVRERLGLDVLFGDDAE
                     ALDRTESAQPALFAIEVALFRLLESWGVRPDFLSGHSVGEIAAAHVAGVLSLEDACTLV
                     AARGRLMQALPMGGVMIALQASEDEVLPLLNGRVSIAAVNGPESVVVAGDEDAAVAIAE
                     SFPDRKSKRLTVSHAFHSPHMDGMLDAFREVVAGLSFAAPRIPVVSNLTGALVTDEMAS
                     PEFWVRHVREAVRFLDGVRALEAAGVTTFIELGPDGVLSAMAQECVSGEGAAFLPVLRK
                     "
     CDS_motif       4822..4869
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43685_0005"
                     /evalue="4.60E-06"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="CHF15_RS43685"
                     /protein_end="581"
                     /protein_start="565"
                     /score="19.0"
                     /tool="antismash"
                     /translation="FTGQGSQRLGMGRELY"
     CDS_motif       4957..5022
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43685_0006"
                     /evalue="9.50E-05"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="CHF15_RS43685"
                     /protein_end="632"
                     /protein_start="610"
                     /score="15.5"
                     /tool="antismash"
                     /translation="LDRTESAQPALFAIEVALFRLL"
     CDS_motif       5032..5154
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43685_0007"
                     /evalue="4.60E-24"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="CHF15_RS43685"
                     /protein_end="676"
                     /protein_start="635"
                     /score="76.8"
                     /tool="antismash"
                     /translation="GVRPDFLSGHSVGEIAAAHVAGVLSLEDACTLVAARGRLMQ"
     CDS_motif       5239..5277
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43685_0008"
                     /evalue="2.60E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="CHF15_RS43685"
                     /protein_end="717"
                     /protein_start="704"
                     /score="14.2"
                     /tool="antismash"
                     /translation="AAVNGPESVVVAG"
     CDS_motif       5494..5526
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43685_0009"
                     /evalue="7.30E-04"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="CHF15_RS43685"
                     /protein_end="800"
                     /protein_start="789"
                     /score="13.0"
                     /tool="antismash"
                     /translation="WVRHVREAVRF"
     aSDomain        5866..6144
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43685_PKS_DH.1"
                     /evalue="7.50E-43"
                     /label="CHF15_RS43685_PKS_DH.1"
                     /locus_tag="CHF15_RS43685"
                     /protein_end="1006"
                     /protein_start="913"
                     /score="138.7"
                     /tool="antismash"
                     /translation="HPLLGATVALADSDGFLFTGRLSLDTHPWLADHAVMGTVLLPGAA
                     FVELAVRAGDQVGCDVLEELTLQAPLVLPERGGVQVQIHVAAPDADGT"
     CDS_motif       5950..6024
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43685_0010"
                     /evalue="5.10E-10"
                     /label="PKSI-DH_m2"
                     /locus_tag="CHF15_RS43685"
                     /protein_end="966"
                     /protein_start="941"
                     /score="31.8"
                     /tool="antismash"
                     /translation="WLADHAVMGTVLLPGAAFVELAVRA"
ORIGIN
        1 gtggaggtgg cgctgttccg gctgctggag tcctggggcg tgaggccgga cttcgtgtcc
       61 ggtcattcgc tcggtgagat cgccgccgct catgtggcag gtgtgttctc gctggaggac
      121 gcgtgcacgc tcgtggccgc tcgcgggcgg ctgatgcagg cgctgccggc cggtggggtg
      181 atgatcgcgc tgcaggcctc ggagggcgag gttctgccgc tgctgaacga tcgggtgagc
      241 atcgcggcgg tgaacggtcc tgagtcggtg gtgatcgccg gtgacgagga cgcggcactc
      301 gcgatcgtgg ggttcttcgc cggccggaag tcgaagcggt tgacggtcag tcacgcgttc
      361 cactccccgg acatggacgg gatgctggcg gacttccgga aggtggcgga gggtctggcc
      421 tacgctgctc ctcgtattcc tctggtgtcc aacttgaccg gggctctggt caccgacgag
      481 gtggcctcgg ccgacttctg ggtccgccac gtccgtgagg cagtgcgctt cctcgatggc
      541 gtccgcgccc tggaggccgt gggcgtcacc acgtatctgg agctcggtcc cgacggtgtg
      601 ctctccgcca tggcccagga ctgtgtgacc ggggacggtg cggccttcgt cccggtcctg
      661 cggggcggtc gtcccgaggc cgggaccgcc gtcaccgcac tcgcccaggc ccacgtccgt
      721 ggtgcgaccg tcgagtgggc acggttcttc gccgggaccg gcgcccgccg cgtcgacctg
      781 cccacctacg ccttccaagg gcggcgctac tggcccgagc ccgccgcccc gctccccgcg
      841 gccgatgccg cacgcgacga ggcggacgcc cgcttctggg aggtggtcga aagcgccgat
      901 ccgaacggga tcgccgacga gttcggcatc gacgcggacc aaccgctgag cgcggtgctg
      961 cccgccctgt cactctggcg ccgagaccgg caggcgcgga cccaggtgga cgcccggcgc
     1021 taccgcgtca cctggcactc gtggccgaac gggggcggga acgcgggcgc gacccccagc
     1081 ggcgtctggc tggcggctct gccggccgcc cacgcggacg atccctggat cagcgcggtg
     1141 acggcgcggg tcgaggcggg cggtgcccgc gtcgtgcccg tcttcctcga caccaccgat
     1201 gccgaacagg acgtactcgc cgcgcgcctg cgcgagcatc tcgccgccgc ggtagcgagc
     1261 ggccccgtgg ccggagtgct gtccctgctg gcgctggacg agcgacctca cccggagcac
     1321 cccggagtcc ccgtcggtct cgcgctgacc gccgcgctcg ccgaggtgct gaccgatgac
     1381 gcctccggcg tcgacggggc cctgtggtgc gtgacccgcg acgccgtggc ggccgttccc
     1441 gcggacggga tcaccggggc cgcgcaggcc caggtctggg gcctgggccg ggtcgtggcc
     1501 ctggaacacc cggaccgctg gggcgggctg gtcgacctgc ccgccgactg cgacgaccgg
     1561 gtgctgcacc gcctggcggc ggtgctcgcc gcagtgggcg acgaggacca gctcgccgtc
     1621 cgcgcgtccg gaaccttcgt acgccgtctg gagcgcgccg cgccccaggg accgggcgcc
     1681 gcgacctgga ccccgcgcgg caccgtgctc gtcaccggag gcaccggcgc gctcggccgc
     1741 cacgtggcgc ggtggctcgc cgagcgcggt gcggagcacc tcgtcctcgt cagccgcaag
     1801 ggcgccgatg cgcccggcgc ggcgcggatc gaggaggaac tgtccggcct cggcgccgag
     1861 gtgacgctcg ccgcctgcga cgtcgccgac cgggccgcgc tcgaagcgct cgtcgcccgg
     1921 ctggccgcgg agggcactcc ggtgcgtgcc gtcgtccacg cggccggcgt ctcccagccg
     1981 cccggcacgc acgccgacct gcgcggcctc gcccacgtcg tggccgcgaa gaccgccggc
     2041 gccgtccacc tcgacgccct cttcgacacg ccggactccc tggacgcctt cgtcctcttc
     2101 tcgtccatcg ccggggtgtg gggcagcggc gggcagggcg cgtacgcggc cgccaacgcg
     2161 ttcctcgacg cgctcgccga gcggcgcggc gcccgcggcc tgcccgcgac cgccctcgcc
     2221 tggggcccgt gggccgacgg gggcatggcg gccgagggcg acgcggagga gcagttgcgc
     2281 cgccgcggac tcccgcccat ggccccggcc gacaacctgc tggcgctgga acgcgccgtg
     2341 acgggccccg acgcggccct gacggtcgcc gaggtggact gggcacgatt cgcacccgcc
     2401 tacgccgcgg cccggccccg ccccttcgtc gccgacctcc ccgaggtccg ggacgcgctg
     2461 cgcgccgacc caccggccgt cggcggcggc acggacaccc cgtcctccgc gacgctccgg
     2521 cggctcgccg aactcaccgg cgacgaccgg cgggtggccc tgctggacct cgtacgcgaa
     2581 cacgcagcgg tggccctcgg acacaccgtc gccgacgcgg tcgccgccga acgcgccttc
     2641 aaggacctcg gcttcgactc gctgaccgcc gtcgaactgc gcaaccgcct gggcgccgcg
     2701 tgcggtctgc gcctgccctc cagtctcgtg ttcgaccacc ccaaccccca ggccctggcc
     2761 cggtacctgc tgcacagcct gttccccgac accactggcg ccgccggtgc cgcctccgcc
     2821 caccccgtgg acaccgaccc gcaggacgcc gaactgcgcc ggacgctggc gaccatcccg
     2881 ctgggccgca tccgcgaggc cggcctgctg gacacactgc tgcggctggc cgcctcggac
     2941 ggaacccccg ccggtgcggc ggaccccgcc gacccctcgg aggccgcgga ctccatcgac
     3001 gcgatggacc tccaggacct cctcgacctg gcactcgacg gccacggcga ccccgacggg
     3061 tcccacggca gcgccacaga cgacgacagc aaccgatcct gacgtgcccc aagtgcggag
     3121 tatgtgatga ccaccccgaa cgaaaaagtc gttgaagcgc tgcgggcatc gctgaaggag
     3181 accgagcggc tgcgccgccg gaaccgggag ctgaccgacg cctcgcgcga gccgatcgcc
     3241 atcgtcgcca tgggatgccg cttccccggt ggcgtccgct cgcccgagga cctgtggaag
     3301 ctcgtcgaca gcggcggcga cggcatcacg ggcttccccg tcgaccgcgg ctgggacatc
     3361 gagtcgctgt acgacccgga ccccgaccac gagggcacca cctacgcccg ccacggcggc
     3421 ttcctccacg acgccggcga cttcgacccc tccttcttcg ggatctcgcc gcgtgaggcg
     3481 ctggccatgg acccgcagca gcgcctgctg ctggagacca cctgggagac cttcgaacgc
     3541 gccgggatcg acccggcgac cctgcgcggc agccgggctg gcgtcttcgt cggcgcctca
     3601 gccaacgcct acggcgccgg cgccggggac ctccccgacg gtgtggaggg ccacctgctc
     3661 accggcaccg cctccagcgt cgtctccggc cggctcgcct acgtcttcgg cctcgaaggc
     3721 cccgccgcca ccatcgacac ggcctgctcg tcctcctccg tcgccctgca catggccgta
     3781 caggcgctgc gccagggcga gtgctccctg gccctggccg cgggcgtcac cgtgctcgcg
     3841 ggccccgacg tcttcgtcga gttcagccgt cagcgcggac tgtcccccga cggccgctgc
     3901 aggtccttcg ccgaagcggc ggacggcacc ggctggtccg agggcgtcgg cgtgctgctg
     3961 gtggagcggc tgtcggacgc gcggcgcaac gggcacccgg tgctggcggt ggtgcggggt
     4021 tcggcggtga accaggacgg cgcgagcaac ggcctgacgg cgccgaacgg cccttcgcag
     4081 cagcgtgtca tccgccaggc tctcgagaac gcgcggctga cccccgccga catcgacgcg
     4141 gtcgaggcgc acggcacggg cacgaccctg ggtgacccca tcgaggcgca ggcgctgctg
     4201 gccacctacg gtcaggaccg cccggcggac cggccgctgc tcctgggctc cctcaagtcc
     4261 aacatcggcc acacccagaa cgcggccgga gtcgcgggca tcatcaagat ggtcatggcg
     4321 atgcgccacg gcgtgctgcc gcagaccctc cacgtcgacg agccgacctc ccacgtcgac
     4381 tggtcgtcgg gagcggtgtc ccttctgacc gaggccgtgc gctggccgga gaccggccgt
     4441 ccgcgccggt tcggcgtctc cgcgttcggc gtcagcggca ccaacgtgca cacgatcctc
     4501 gaggaggccc cggagcccga ggcgacggcc ccggcacccg cagtcggggg tgtggtgccg
     4561 tggctgctgt cggggaaggg gcacgaggct ctgcgcgagc aggcccgccg cctgcacgcg
     4621 tatgtcgggg agaaccccgg gtcgcgtgcg gtggacgtcg cgcactcgct ggcggtggga
     4681 cgtgcctcgt tcgacgaccg cgccgtcgtc gtcgccgagg accgcgcggg actgctgtcg
     4741 ggcctcgccg ccctgtccga gggcgaagtg acgccgggcg tgctgaccgg ctcgcccgtc
     4801 gcgggcaagg tggccttcct gttcacgggg caggggagtc agcggctggg gatggggcgt
     4861 gagctgtacg cggcctatcc ggtgttcgca cgggcgcttg acgaggtgcg tgagcggctg
     4921 gggctggacg tgctgttcgg cgacgacgcg gaggcgttgg accggacgga gtccgcgcag
     4981 cccgcgttgt tcgcgatcga ggtggcgctg ttccggctgc tggagagctg gggcgtgcgg
     5041 ccggacttcc tgtccgggca ttcggtaggt gagatcgccg ccgcgcatgt ggccggggtg
     5101 ctgtccctgg aggatgcgtg cacgctggtg gcggcgcgtg gccggttgat gcaggcgctg
     5161 ccgatgggcg gtgtgatgat cgcgctgcag gcctcggagg acgaggtcct gccgctgctg
     5221 aacggccgcg tgagtattgc cgcggtgaat ggtccggagt cggtggtcgt cgcgggtgac
     5281 gaggacgccg cggtcgcgat cgcggagtcc ttcccggacc ggaagtcgaa gcgtctgacg
     5341 gtcagtcacg cattccactc cccgcacatg gacgggatgc tcgacgcctt ccgcgaggtg
     5401 gtggcggggt tgtcgttcgc cgctccgcgc atcccggtcg tctcgaacct gaccggtgcc
     5461 ctggtcacgg acgagatggc ctcgccggag ttctgggtgc ggcatgtccg tgaggccgtg
     5521 cgtttcctgg acggggtgcg tgctctggag gccgcgggcg tcaccacgtt cattgaactc
     5581 ggcccggacg gcgtgctgtc ggcgatggcg caggagtgcg tgagcggcga aggtgctgct
     5641 tttcttcccg tgctgcgcaa gggccgttcg gaggccgaga cggtcgttac cgcggtcgcg
     5701 caggcccacg cctggggcat cgcggtcgac tggccggcgt tcttcgccgg gaccggcgcg
     5761 cggcgtgtcg atctgccgac ctatgccttc cagcggcagc gctattggat cgagtcgggc
     5821 cgcggcgccg gcgatctgcg cgctgtcggc ctggaggccc tgggtcaccc gctgctgggc
     5881 gcgaccgtgg cgctcgccga ctccgacggc ttcctcttca ccggccgtct ctccctcgac
     5941 acgcacccct ggctcgccga ccacgccgtc atgggcaccg tcctgcttcc cggcgcggcc
     6001 tttgtggaac tggccgttcg tgccggtgac caggtcggtt gtgacgtgct ggaggaactc
     6061 accctccagg ccccgctcgt tctccccgag cgcggcggcg tgcaggtgca gatccacgtg
     6121 gccgcgcccg acgcggacgg cacaggtcg
//
