LOCUS       NZ_FZOF01000070         6179 bp    DNA     linear   CON 02-JAN-2025
DEFINITION  Actinacidiphila glaucinigra strain CGMCC 4.1858, whole genome
            shotgun sequence.
ACCESSION   NZ_FZOF01000070
VERSION     NZ_FZOF01000070.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinacidiphila glaucinigra
  ORGANISM  Actinacidiphila glaucinigra
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Actinacidiphila.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2017) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900188405.1-RS_2025_01_02
            Annotation Date                   :: 01/02/2025 02:14:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,793
            CDSs (total)                      :: 8,707
            Genes (coding)                    :: 8,547
            CDSs (with protein)               :: 8,547
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 1 (5S, 16S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 47 of 160
            Pseudo Genes (incomplete)         :: 136 of 160
            Pseudo Genes (internal stop)      :: 6 of 160
            Pseudo Genes (multiple problems)  :: 29 of 160
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:25:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6179
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FZOF01000070.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..3066
                     /locus_tag="CHF15_RS43670"
                     /old_locus_tag="SAMN05216252_1701"
     CDS             <1..3066
                     /NRPS_PKS="Domain: PKS_AT (0-221). E-value: 4e-81. Score:
                     264.6. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43670_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_KR (574-760). E-value: 5.6e-59.
                     Score: 191.1. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43670_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (867-940). E-value: 2.5e-33. Score:
                     106.2. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43670_ACP.1"
                     /NRPS_PKS="Domain: PKS_Docking_Cterm (949-1020). E-value:
                     1.5e-19. Score: 62.6. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43670_PKS_Docking_Cterm.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009717825.1"
                     /locus_tag="CHF15_RS43670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216252_1701"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_143681799.1"
                     /sec_met_domain="PKS_AT (E-value: 1.3e-82, bitscore: 264.6,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 1.9e-58, bitscore:
                     184.4, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.7e-17, bitscore:
                     51.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VEVALFRLLESWGVRPDFVSGHSLGEIAAAHVAGVFSLEDACTLV
                     AARGRLMQALPAGGVMIALQASEGEVLPLLNDRVSIAAVNGPESVVIAGDEDAALAIVG
                     FFAGRKSKRLTVSHAFHSPDMDGMLADFRKVAEGLAYAAPRIPLVSNLTGALVTDEVAS
                     ADFWVRHVREAVRFLDGVRALEAVGVTTYLELGPDGVLSAMAQDCVTGDGAAFVPVLRG
                     GRPEAGTAVTALAQAHVRGATVEWARFFAGTGARRVDLPTYAFQGRRYWLEEADAAVPA
                     AGADGYSGAADAPFWDAVDNADLAALAGTLGVDTDQPLGVLLPALSAWRRQRQEESVAD
                     GWRYGVTWRPIGVPSASSPSGTWIVVSTAEDAGRGLPAVAEALAARGADVRQLVAPAAA
                     TDREALAGLLRGITGGDGLGAVAGVVSLLAFAEQPHPVHAAAPAGLLLTAALVQALGDA
                     GITAPLWCVTSGAVATSAADRLTSVAQAQVWGLGRVVALEHPDRWGGLVDLPGTADERV
                     LDRLLGVLAGGCGDEDQVALRSGGLLARRISHEAGVTRGAGDGGLERAEGRTPWRPRGT
                     VLVTGGTGALGGHVARWLAANGAEHLVLVSRRGPQAPGADELVAGIGALGVRATAVACD
                     VTDREAVADLLARLRGPHTDGTGGPGLTAVFHTAGTGQFAPLAETGPADVADVVAAKVA
                     GASHLDELLGTDELDAFVLFSSIAGVWGSGGQAAYAAANAHLDALAQHRRARGMTATSV
                     AWGPWAEGGLVADDGAEEQLRRRGLPAMAPKPSLAALQRALDGDETTVIVADVDWDRFA
                     PAFTAARSRPLIGDLPEVRRALAADQDGGPAPTGEVPDLAAQLRDLDAPETERVVLDLV
                     RAQVAAVLGHQGTASVDAARAFKDLGFDSLTAVELRNRLQTATGLRLPAGLVFDHPTPA
                     ALAAYVRAELVGEDTTPALPALAEIDKLEFLLSSVPEDTAERARVTARLESLLANWNRA
                     EREAIGEDEDSEIASASADDLFDIINKEFGKS"
     source          1..6179
                     /db_xref="taxon:235986"
                     /mol_type="genomic DNA"
                     /note="contig: Ga0079895_170"
                     /organism="Actinacidiphila glaucinigra"
                     /strain="CGMCC 4.1858"
                     /type_material="type strain of Streptomyces glauciniger"
     region          1..6179
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..6179
                     /SMILES="CC(O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6179
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[3138:>6179](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3139..>6179
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     aSDomain        4..666
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43670_PKS_AT.1"
                     /evalue="4.00E-81"
                     /label="CHF15_RS43670_PKS_AT.1"
                     /locus_tag="CHF15_RS43670"
                     /protein_end="221"
                     /protein_start="0"
                     /score="264.6"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="VEVALFRLLESWGVRPDFVSGHSLGEIAAAHVAGVFSLEDACTLV
                     AARGRLMQALPAGGVMIALQASEGEVLPLLNDRVSIAAVNGPESVVIAGDEDAALAIVG
                     FFAGRKSKRLTVSHAFHSPDMDGMLADFRKVAEGLAYAAPRIPLVSNLTGALVTDEVAS
                     ADFWVRHVREAVRFLDGVRALEAVGVTTYLELGPDGVLSAMAQDCVTGDGAAFVPVLR"
     aSModule        4..2823
                     /complete
                     /domains="nrpspksdomains_CHF15_RS43670_PKS_AT.1"
                     /domains="nrpspksdomains_CHF15_RS43670_PKS_KR.1"
                     /domains="nrpspksdomains_CHF15_RS43670_ACP.1"
                     /locus_tags="CHF15_RS43670"
                     /monomer_pairings="mal -> ohmal"
                     /starter_module
                     /tool="antismash"
                     /type="pks"
     CDS_motif       40..162
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43670_0001"
                     /evalue="1.60E-24"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="CHF15_RS43670"
                     /protein_end="53"
                     /protein_start="12"
                     /score="78.3"
                     /tool="antismash"
                     /translation="GVRPDFVSGHSLGEIAAAHVAGVFSLEDACTLVAARGRLMQ"
     CDS_motif       247..285
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43670_0002"
                     /evalue="1.90E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="CHF15_RS43670"
                     /protein_end="94"
                     /protein_start="81"
                     /score="14.6"
                     /tool="antismash"
                     /translation="AAVNGPESVVIAG"
     CDS_motif       433..465
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43670_0003"
                     /evalue="1.90E-03"
                     /label="PKSI-AT-M_m7"
                     /locus_tag="CHF15_RS43670"
                     /protein_end="154"
                     /protein_start="143"
                     /score="10.9"
                     /tool="antismash"
                     /translation="PRIPLVSNLTG"
     CDS_motif       502..534
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43670_0004"
                     /evalue="7.40E-04"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="CHF15_RS43670"
                     /protein_end="177"
                     /protein_start="166"
                     /score="13.0"
                     /tool="antismash"
                     /translation="WVRHVREAVRF"
     CDS_motif       1390..1413
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43670_0005"
                     /evalue="8.50E+01"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="CHF15_RS43670"
                     /protein_end="470"
                     /protein_start="462"
                     /score="-2.6"
                     /tool="antismash"
                     /translation="PLWCVTSG"
     aSDomain        1726..2283
                     /ASF="KR domain putatively catalyzing L-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43670_PKS_KR.1"
                     /evalue="5.60E-59"
                     /label="CHF15_RS43670_PKS_KR.1"
                     /locus_tag="CHF15_RS43670"
                     /protein_end="760"
                     /protein_start="574"
                     /score="191.1"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: A1"
                     /tool="antismash"
                     /translation="GTVLVTGGTGALGGHVARWLAANGAEHLVLVSRRGPQAPGADELV
                     AGIGALGVRATAVACDVTDREAVADLLARLRGPHTDGTGGPGLTAVFHTAGTGQFAPLA
                     ETGPADVADVVAAKVAGASHLDELLGTDELDAFVLFSSIAGVWGSGGQAAYAAANAHLD
                     ALAQHRRARGMTATSVAWGPWAE"
     CDS_motif       1729..1827
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43670_0006"
                     /evalue="4.70E-15"
                     /label="PKSI-KR_m1"
                     /locus_tag="CHF15_RS43670"
                     /protein_end="608"
                     /protein_start="575"
                     /score="48.1"
                     /tool="antismash"
                     /translation="TVLVTGGTGALGGHVARWLAANGAEHLVLVSRR"
     CDS_motif       2125..2235
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43670_0007"
                     /evalue="1.70E-20"
                     /label="PKSI-KR_m4"
                     /locus_tag="CHF15_RS43670"
                     /protein_end="744"
                     /protein_start="707"
                     /score="65.6"
                     /tool="antismash"
                     /translation="LDAFVLFSSIAGVWGSGGQAAYAAANAHLDALAQHRR"
     aSDomain        2605..2823
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43670_ACP.1"
                     /evalue="2.50E-33"
                     /label="CHF15_RS43670_ACP.1"
                     /locus_tag="CHF15_RS43670"
                     /protein_end="940"
                     /protein_start="867"
                     /score="106.2"
                     /tool="antismash"
                     /translation="LDLVRAQVAAVLGHQGTASVDAARAFKDLGFDSLTAVELRNRLQT
                     ATGLRLPAGLVFDHPTPAALAAYVRAEL"
     aSDomain        2851..3063
                     /aSDomain="PKS_Docking_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43670_PKS_Docking_Cterm.
                     1"
                     /evalue="1.50E-19"
                     /label="CHF15_RS43670_PKS_Docking_Cterm.1"
                     /locus_tag="CHF15_RS43670"
                     /protein_end="1020"
                     /protein_start="949"
                     /score="62.6"
                     /tool="antismash"
                     /translation="PALAEIDKLEFLLSSVPEDTAERARVTARLESLLANWNRAEREAI
                     GEDEDSEIASASADDLFDIINKEFGK"
     misc_feature    3116..3131
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 23.65"
                     /tool="antismash"
     misc_feature    complement(3116..3132)
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.14"
                     /tool="antismash"
     misc_feature    complement(3128..3143)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 19.52"
                     /tool="antismash"
     gene            3139..>6179
                     /locus_tag="CHF15_RS43675"
                     /old_locus_tag="SAMN05216252_1702"
     CDS             3139..>6179
                     /NRPS_PKS="Domain: PKS_Docking_Nterm (5-32). E-value:
                     2e-12. Score: 38.4. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43675_PKS_Docking_Nterm.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (37-463). E-value:
                     7.5e-181. Score: 593.6. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43675_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (567-851). E-value: 7.5e-106.
                     Score: 345.8. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43675_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (919-1012). E-value: 7.6e-43.
                     Score: 138.7. Matches aSDomain:
                     nrpspksdomains_CHF15_RS43675_PKS_DH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060790.1"
                     /locus_tag="CHF15_RS43675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216252_1702"
                     /product="type I polyketide synthase"
                     /protein_id="WP_143681800.1"
                     /sec_met_domain="mod_KS (E-value: 1.5e-229, bitscore:
                     749.1, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 1.6e-90, bitscore:
                     290.1, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 2.5e-107, bitscore:
                     345.8, seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSANEEKLLENLKWMTAELRRSRRRVQEIEADAREPVAIVAMSC
                     RFPGGVGSPEELWRLVDEGGDAISGFPADRGWDVTSLADPDPDRKGAFYNTGGGFLDDA
                     TVFDPGFFGISPREALAMDPQQRQLLEVSWEAFERAGIDPSTVHGSRTGVYVGASAMGY
                     GADLGEVPEGLEGLLLTGGATSVLSGRVSYVFGLEGPAATVDTACSSSLVALHLAVQAL
                     RQRECSLALVGGVCVMPNTDVFVEFSRQRGLAPDGRCKSFAASADGTGWSEGVGVLLVE
                     RLSDARRNGHPVLAVVRGSAVNQDGASNGLTAPNGPSQQRVIRQALENAQLSAAEIDVV
                     EAHGTGTTLGDPIEAQALLATYGQDRPEGNPLRLGSLKSNIGHTQAAAGVAGIIKMVMA
                     MRHGVLPRTLHVDEPTPGVDWSAGAVALLTEPMPWSGSGAPRRAAVSAFGVSGTNAHTI
                     LEEAPEPEEVPEPSAASPARVVPWLLSGKGRDALREQARRLHAYVGENPGLRAVDAAYS
                     LAVGRASFEDRAVVVAEDRAGVLAGLGALSEGDPTTGVVTGSPVAGKVAFLFTGQGSQR
                     LGMGRELYDTHPGFADALDGVCARLGLSLKDVMFGDDAEALDRTEFAQPALFAVEVALF
                     RLLESWGVRPDFVSGHSVGEIAAAHVAGVLSLEDACTLVAARGRLMQALPTGGVMIALQ
                     ASEDEVLPLLNARVSVAAVNGPQAVVIAGDEDAAVAIVEALGDRKSKRLTVSHAFHSPH
                     MDGMLDAFREVVAGLSFAAPRIPVVSNLTGAVVTDEMASPEFWVRHVREAVRFLDGVRA
                     LEAAGVTTFIELGPDGVLSAMAQECVTGEVAAFVPALRKGRSEAETVVTALAQAHAWGI
                     AVDWPAFFAGTGARRVDLPTYAFQRQRYWIESGRGAGDLRAVGLEALGHPLLGATVALA
                     DSDGFLFTGRLSLDTHPWLADHAVMGTVLLPGAAFVELAVRAGDQVGCDVLEELTLQAP
                     LVLPERGGVQVQIHVAAPDADGTGR"
     aSDomain        3154..3234
                     /aSDomain="PKS_Docking_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43675_PKS_Docking_Nterm.
                     1"
                     /evalue="2.00E-12"
                     /label="CHF15_RS43675_PKS_Docking_Nterm.1"
                     /locus_tag="CHF15_RS43675"
                     /protein_end="32"
                     /protein_start="5"
                     /score="38.4"
                     /tool="antismash"
                     /translation="EEKLLENLKWMTAELRRSRRRVQEIEA"
     misc_feature    complement(3160..3174)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.98"
                     /tool="antismash"
     aSDomain        3250..4527
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43675_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="7.50E-181"
                     /label="CHF15_RS43675_PKS_KS.1"
                     /locus_tag="CHF15_RS43675"
                     /protein_end="463"
                     /protein_start="37"
                     /score="593.6"
                     /tool="antismash"
                     /translation="VAIVAMSCRFPGGVGSPEELWRLVDEGGDAISGFPADRGWDVTSL
                     ADPDPDRKGAFYNTGGGFLDDATVFDPGFFGISPREALAMDPQQRQLLEVSWEAFERAG
                     IDPSTVHGSRTGVYVGASAMGYGADLGEVPEGLEGLLLTGGATSVLSGRVSYVFGLEGP
                     AATVDTACSSSLVALHLAVQALRQRECSLALVGGVCVMPNTDVFVEFSRQRGLAPDGRC
                     KSFAASADGTGWSEGVGVLLVERLSDARRNGHPVLAVVRGSAVNQDGASNGLTAPNGPS
                     QQRVIRQALENAQLSAAEIDVVEAHGTGTTLGDPIEAQALLATYGQDRPEGNPLRLGSL
                     KSNIGHTQAAAGVAGIIKMVMAMRHGVLPRTLHVDEPTPGVDWSAGAVALLTEPMPWSG
                     SGAPRRAAVSAFGVSGTNAHTILEEAP"
     aSModule        3250..6174
                     /domains="nrpspksdomains_CHF15_RS43675_PKS_KS.1"
                     /domains="nrpspksdomains_CHF15_RS43675_PKS_AT.1"
                     /domains="nrpspksdomains_CHF15_RS43675_PKS_DH.1"
                     /incomplete
                     /locus_tags="CHF15_RS43675"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       3733..3780
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43675_0001"
                     /evalue="1.70E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="CHF15_RS43675"
                     /protein_end="214"
                     /protein_start="198"
                     /score="19.9"
                     /tool="antismash"
                     /translation="GPAATVDTACSSSLVA"
     CDS_motif       4156..4200
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43675_0002"
                     /evalue="1.80E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="CHF15_RS43675"
                     /protein_end="354"
                     /protein_start="339"
                     /score="13.5"
                     /tool="antismash"
                     /translation="VEAHGTGTTLGDPIE"
     CDS_motif       4261..4305
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43675_0003"
                     /evalue="1.00E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="CHF15_RS43675"
                     /protein_end="389"
                     /protein_start="374"
                     /score="11.6"
                     /tool="antismash"
                     /translation="GSLKSNIGHTQAAAG"
     aSDomain        4840..5691
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43675_PKS_AT.1"
                     /evalue="7.50E-106"
                     /label="CHF15_RS43675_PKS_AT.1"
                     /locus_tag="CHF15_RS43675"
                     /protein_end="851"
                     /protein_start="567"
                     /score="345.8"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFTGQGSQRLGMGRELYDTHPGFADALDGVCARLGLSLKDVMFGD
                     DAEALDRTEFAQPALFAVEVALFRLLESWGVRPDFVSGHSVGEIAAAHVAGVLSLEDAC
                     TLVAARGRLMQALPTGGVMIALQASEDEVLPLLNARVSVAAVNGPQAVVIAGDEDAAVA
                     IVEALGDRKSKRLTVSHAFHSPHMDGMLDAFREVVAGLSFAAPRIPVVSNLTGAVVTDE
                     MASPEFWVRHVREAVRFLDGVRALEAAGVTTFIELGPDGVLSAMAQECVTGEVAAFVPA
                     LRK"
     CDS_motif       4843..4890
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43675_0004"
                     /evalue="4.60E-06"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="CHF15_RS43675"
                     /protein_end="584"
                     /protein_start="568"
                     /score="19.0"
                     /tool="antismash"
                     /translation="FTGQGSQRLGMGRELY"
     CDS_motif       4987..5052
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43675_0005"
                     /evalue="4.00E-05"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="CHF15_RS43675"
                     /protein_end="638"
                     /protein_start="616"
                     /score="16.6"
                     /tool="antismash"
                     /translation="LDRTEFAQPALFAVEVALFRLL"
     CDS_motif       5062..5184
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43675_0006"
                     /evalue="2.70E-24"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="CHF15_RS43675"
                     /protein_end="682"
                     /protein_start="641"
                     /score="77.5"
                     /tool="antismash"
                     /translation="GVRPDFVSGHSVGEIAAAHVAGVLSLEDACTLVAARGRLMQ"
     CDS_motif       5269..5307
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43675_0007"
                     /evalue="2.80E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="CHF15_RS43675"
                     /protein_end="723"
                     /protein_start="710"
                     /score="14.1"
                     /tool="antismash"
                     /translation="AAVNGPQAVVIAG"
     CDS_motif       5455..5487
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43675_0008"
                     /evalue="4.60E-03"
                     /label="PKSI-AT-M_m7"
                     /locus_tag="CHF15_RS43675"
                     /protein_end="783"
                     /protein_start="772"
                     /score="9.8"
                     /tool="antismash"
                     /translation="PRIPVVSNLTG"
     CDS_motif       5524..5556
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43675_0009"
                     /evalue="7.30E-04"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="CHF15_RS43675"
                     /protein_end="806"
                     /protein_start="795"
                     /score="13.0"
                     /tool="antismash"
                     /translation="WVRHVREAVRF"
     aSDomain        5896..6174
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS43675_PKS_DH.1"
                     /evalue="7.60E-43"
                     /label="CHF15_RS43675_PKS_DH.1"
                     /locus_tag="CHF15_RS43675"
                     /protein_end="1012"
                     /protein_start="919"
                     /score="138.7"
                     /tool="antismash"
                     /translation="HPLLGATVALADSDGFLFTGRLSLDTHPWLADHAVMGTVLLPGAA
                     FVELAVRAGDQVGCDVLEELTLQAPLVLPERGGVQVQIHVAAPDADGT"
     CDS_motif       5980..6054
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CHF15_RS43675_0010"
                     /evalue="5.20E-10"
                     /label="PKSI-DH_m2"
                     /locus_tag="CHF15_RS43675"
                     /protein_end="972"
                     /protein_start="947"
                     /score="31.8"
                     /tool="antismash"
                     /translation="WLADHAVMGTVLLPGAAFVELAVRA"
ORIGIN
        1 gtggaggtgg cgctgttccg gctgctggag tcctggggcg tgaggccgga cttcgtgtcc
       61 ggtcattcgc tcggtgagat cgccgccgct catgtggcag gtgtgttctc gctggaggac
      121 gcgtgcacgc tcgtggccgc tcgcgggcgg ctgatgcagg cgctgccggc cggtggggtg
      181 atgatcgcgc tgcaggcctc ggagggcgag gttctgccgc tgctgaacga tcgggtgagc
      241 atcgcggcgg tgaacggtcc tgagtcggtg gtgatcgccg gtgacgagga cgcggcactc
      301 gcgatcgtgg ggttcttcgc cggccggaag tcgaagcggt tgacggtcag tcacgcgttc
      361 cactccccgg acatggacgg gatgctggcg gacttccgga aggtggcgga gggtctggcc
      421 tacgctgctc ctcgtattcc tctggtgtcc aacttgaccg gggctctggt caccgacgag
      481 gtggcctcgg ccgacttctg ggtccgccac gtccgtgagg cagtgcgctt cctcgatggc
      541 gtccgcgccc tggaggccgt gggcgtcacc acgtatctgg agctcggtcc cgacggtgtg
      601 ctctccgcca tggcccagga ctgtgtgacc ggggacggtg cggccttcgt cccggtcctg
      661 cggggcggtc gtcccgaggc cgggaccgcc gtcaccgcac tcgcccaggc ccacgtccgt
      721 ggtgcgaccg tcgagtgggc acggttcttc gccgggaccg gcgcccgccg cgtcgacctg
      781 cccacctacg ccttccaagg gcggcgctac tggctggagg aggccgacgc agcggtgccc
      841 gccgcaggcg ccgacgggta ctccggcgcg gccgacgcac cgttctggga tgcggtcgac
      901 aacgccgacc tcgccgcgct cgcgggcacc ctgggcgtcg acacggacca gccactgggc
      961 gtgctgctgc ccgcgctgtc ggcttggcgc cggcagcgcc aggaggagtc cgtggcggac
     1021 ggctggcgct acggggtgac ctggaggccg atcggcgtcc cgtccgccag cagcccgtcc
     1081 ggcacgtgga tcgtcgtgag caccgcggag gacgcgggcc gcggcctgcc cgccgtcgcc
     1141 gaggccctgg cggcccgcgg cgcggacgta cgccaactgg tcgcccccgc cgccgcgacc
     1201 gaccgtgagg cactggccgg cctgctgcgc gggatcaccg gcggcgacgg gctcggcgct
     1261 gtcgcgggcg tcgtctcgct gctggccttt gccgagcagc cgcacccggt gcatgccgcg
     1321 gcgccggccg ggctgttgct gacggcggct ctggtgcagg cactgggcga cgccggcatc
     1381 acggcaccgc tgtggtgtgt cacatcaggg gccgtggcga cctcggcggc ggaccgcttg
     1441 accagtgtcg cgcaggcgca ggtgtggggc ctgggccggg tcgtggccct ggagcacccc
     1501 gaccgctggg gcgggctcgt ggatctgccg ggcaccgcgg acgaacgcgt cctcgaccgg
     1561 ctgctcggcg tactcgcggg cggctgcggg gacgaggacc aggtcgcgct acgcagcgga
     1621 gggctgctgg cccggcgtat cagccacgag gccggcgtca cgcggggcgc gggggacggc
     1681 ggccttgagc gcgcggaggg gcgtacgccg tggcgtcccc gcggcaccgt gctcgtcacc
     1741 ggcgggacgg gggctctcgg cggccacgtg gcccgatggc tggcggcgaa cggggcggaa
     1801 cacctggtgc tggtcagccg gcgcgggccg caggcgccgg gcgcggacga actggtcgcc
     1861 gggatcggcg ccctgggcgt ccgggccacg gccgtggcct gcgacgtgac cgatcgcgag
     1921 gccgtcgccg acctgctggc gcggctgcgc ggaccgcaca ccgacggaac gggcgggccg
     1981 ggcctcaccg cggtcttcca cacggcgggc accgggcagt tcgcgccgct ggcggagacc
     2041 ggccccgccg acgtggccga cgtcgtcgcc gccaaggtcg cgggagcctc ccacctggac
     2101 gaactcctcg gcacggacga gctggacgcc ttcgtcctct tctcctccat cgccggggtg
     2161 tggggcagcg gcggccaggc cgcctacgcc gccgccaacg cccacctcga cgccctggcg
     2221 cagcaccgcc gggcccgcgg gatgacggcg acctcggtgg cctggggccc ctgggcggag
     2281 ggcggcctcg tcgccgacga cggcgcggag gagcaactgc gccggcgcgg actgcccgcg
     2341 atggcgccga agccgtccct cgcggcgctc cagcgcgccc tggacgggga cgagaccacg
     2401 gtgatcgtgg ccgacgtcga ctgggaccgc ttcgccccgg ccttcaccgc ggccaggtcc
     2461 cgcccgctca tcggcgacct gcccgaagtg cgccgcgcgc tcgccgccga ccaggacggc
     2521 ggccccgcac ccacgggcga agtccccgac ctggcggcgc agttgcggga tctggacgca
     2581 ccggagacgg agcgggtcgt gctcgacctg gtccgcgcgc aggtggcggc cgtcctcggc
     2641 caccagggca cggcgtccgt cgacgccgca cgtgccttca aggacctggg cttcgactcg
     2701 ctgaccgccg tcgaactgcg caaccggctg cagaccgcga ccgggctgcg cctgccggcc
     2761 ggcctcgtct tcgaccatcc gacgcccgcg gcactggcgg cgtacgtccg ggcggaactc
     2821 gtcggcgagg acaccacccc cgcactgccg gccctcgcgg agatcgacaa gctggaattc
     2881 ctcctgtcgt ccgtccccga ggacaccgca gaacgcgccc gcgtcaccgc acggctcgaa
     2941 tcgctcctgg cgaactggaa cagggcggaa cgagaggcca tcggagagga cgaggacagc
     3001 gaaatcgcat cggcttccgc cgacgacctc ttcgacatca tcaacaaaga attcggaaaa
     3061 tcctgagccg atggctgttc cgacgaccca tccgatgcca gcacggaatt ccgcgatacc
     3121 ccagggggtg acgttccggt gaccagcgcg aacgaagaga agcttctgga aaacctgaag
     3181 tggatgacgg ccgagctgcg ccgctcccgt cgccgcgtgc aggagatcga ggccgacgcg
     3241 cgggaacccg tcgccatcgt cgcgatgagc tgccgtttcc ccggtggggt cggatccccc
     3301 gaggaattgt ggcgactcgt cgacgaaggc ggcgacgcca tttcgggatt ccccgcggac
     3361 cgcggctggg atgtcacgtc cctcgccgat cccgatcccg accgcaaggg cgccttctac
     3421 aacaccggcg gcggattcct cgacgacgcc accgtgttcg atcccggatt cttcgggatc
     3481 tcgccgcgcg aggcgctcgc gatggacccg cagcagcgcc agttgctgga ggtctcctgg
     3541 gaggcgttcg agcgcgccgg catcgacccc tcgacggtgc acggcagccg caccggcgtc
     3601 tacgtcggcg cgagcgcgat gggatacgga gccgacctgg gggaggtccc ggaaggactc
     3661 gaggggctgc tgctgaccgg cggcgccacc agtgtcctgt ccggccgcgt cagttacgtc
     3721 ttcgggctgg aaggcccggc cgccaccgtc gacaccgcgt gctcgtcctc cctcgtcgcc
     3781 ctccatctgg ccgtccaggc cctgcgccag cgcgagtgct cgctcgccct ggtcggcggc
     3841 gtgtgcgtca tgcccaacac ggacgtgttc gtcgagttca gccgccagcg gggcctggcg
     3901 cccgacgggc gctgcaagtc cttcgccgcc tccgccgacg gcaccggctg gtccgagggc
     3961 gtcggcgtgc tgctggtgga gcggctgtcg gacgcgcggc gcaacgggca cccggtgctg
     4021 gcggtggtgc ggggttcggc ggtgaaccag gacggcgcga gcaacggcct gacggcgccg
     4081 aacggcccgt cgcagcagcg tgtcatccgc caggctctcg agaacgccca gctctccgcc
     4141 gcggagatcg acgtcgtcga ggcgcacggc acgggcacga ccctgggtga ccccatcgag
     4201 gcgcaggcgc tgctggccac ctacggtcag gaccggcccg agggaaaccc cctgcgcctg
     4261 ggctccctca agtccaacat cgggcacacc caggccgcgg ccggtgtcgc cggcatcatc
     4321 aagatggtca tggcgatgcg ccacggggtc ctccccagga ccctccacgt ggacgagccc
     4381 acccccggcg tcgactggtc ggccggcgcg gtcgccctcc tgaccgagcc catgccgtgg
     4441 tccggttccg gtgcgccgcg tcgcgccgcc gtgtccgcgt tcggtgtcag cggcaccaac
     4501 gcccacacga tcctcgagga ggccccggag cccgaggagg tcccggagcc gtcggccgcc
     4561 tccccggcgc gggtggtgcc gtggctgctg tcggggaagg ggcgcgacgc cttgcgcgag
     4621 caggcccgcc gcctgcacgc gtatgtcggg gagaacccgg ggttgcgtgc cgtggacgcc
     4681 gcatactcgc tggccgtggg gcgtgcctcg ttcgaggacc gcgcggttgt cgtggccgag
     4741 gatcgtgccg gcgtgctggc gggtctgggt gcgctgtccg agggcgaccc gacgacgggc
     4801 gtggtgaccg gctcgcccgt cgcgggcaag gtggccttcc tgttcaccgg gcagggcagc
     4861 cagcgcctcg gcatggggcg ggagttgtac gacacccatc ctgggttcgc cgatgctctg
     4921 gacggggtgt gcgcgcgcct cggcctgtcg ctgaaggacg tcatgttcgg tgacgacgcg
     4981 gaggcgttgg atcggacgga gttcgcgcag ccggcgttgt tcgcggtgga ggtggcgctg
     5041 ttccggctgc tggagagctg gggcgtgcgg cccgacttcg tgtcgggtca ttcggtgggc
     5101 gagatcgcgg ccgcgcatgt ggcgggggtg ttgtccctgg aggatgcgtg cacgctggtg
     5161 gcggcgcgtg gccggttgat gcaggcgctg ccgacgggtg gcgtgatgat cgcgctgcag
     5221 gcgtcggagg acgaggttct gccgctgctg aacgcgcggg tgagcgtcgc ggctgtcaac
     5281 gggccgcagg ctgtggtgat cgcgggtgac gaggatgccg cggtcgcgat cgtggaggcc
     5341 ttgggtgacc ggaagtcgaa gcgtctgacg gtcagccacg ctttccactc cccgcacatg
     5401 gacgggatgc tcgatgcctt ccgcgaggtg gtggcggggt tgtcgttcgc cgctccgcgt
     5461 atcccggtgg tctccaacct caccggcgcg gtcgtcacgg acgagatggc ctcgccggag
     5521 ttctgggtgc gccacgtccg tgaggccgtg cgtttcctgg acggcgtgcg cgccctggag
     5581 gccgcgggcg tcaccacgtt cattgaactc ggtccggacg gcgtgctgtc ggcgatggcg
     5641 caggagtgcg tgaccggcga agtcgccgcc ttcgttcccg cgctgcgcaa gggccgttcc
     5701 gaggccgaga cggtcgtcac cgcgctcgcc caggcccacg cctggggcat cgcggtcgac
     5761 tggccggcgt tcttcgccgg gaccggcgcg cggcgtgtcg acctgccgac ctacgccttc
     5821 cagcggcagc gctactggat cgagtcgggc cgcggcgccg gcgatctgcg cgctgtcggc
     5881 ctggaggccc tgggtcaccc gctgctgggc gcgaccgtgg cgctcgccga ctccgacggc
     5941 ttcctcttca ccggccgtct ctccctcgac acgcacccct ggctcgccga ccacgccgtc
     6001 atgggcaccg tcctgcttcc cggcgcggcc tttgtggaac tggccgttcg tgccggtgac
     6061 caggtcggtt gtgacgtgct ggaggaactc accctccagg ccccgctcgt tctccccgag
     6121 cgcggcggcg tgcaggtgca gatccacgtg gccgcgcccg acgcggacgg cacaggtcg
//
