LOCUS       NZ_FZOF01000001        10399 bp    DNA     linear   CON 02-JAN-2025
DEFINITION  Actinacidiphila glaucinigra strain CGMCC 4.1858, whole genome
            shotgun sequence.
ACCESSION   NZ_FZOF01000001
VERSION     NZ_FZOF01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinacidiphila glaucinigra
  ORGANISM  Actinacidiphila glaucinigra
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Actinacidiphila.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2017) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900188405.1-RS_2025_01_02
            Annotation Date                   :: 01/02/2025 02:14:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,793
            CDSs (total)                      :: 8,707
            Genes (coding)                    :: 8,547
            CDSs (with protein)               :: 8,547
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 1 (5S, 16S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 47 of 160
            Pseudo Genes (incomplete)         :: 136 of 160
            Pseudo Genes (internal stop)      :: 6 of 160
            Pseudo Genes (multiple problems)  :: 29 of 160
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:25:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 777867
            Orig. end                         :: 788266
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FZOF01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="3"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    736..749
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.76"
                     /tool="antismash"
     gene            complement(890..1657)
                     /locus_tag="CHF15_RS03540"
                     /old_locus_tag="SAMN05216252_101708"
     CDS             complement(890..1657)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381154.1"
                     /locus_tag="CHF15_RS03540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216252_101708"
                     /product="IclR family transcriptional regulator"
                     /protein_id="WP_089222062.1"
                     /transl_table=11
                     /translation="MAAGEGGAQVKSAVRTVELLEFFAGQPGMHSLAAVQEAVGYPKSS
                     LYMLLRTLVELGWIETDATGTRYGIGVRALLVGTSYIDGDEVVAAARPTLDRLSDDTSE
                     TIHLARLDGTSVVYLATRQSSHYLRPFTRVGRRLPAHSTSLGKALLATYTDEQVRKLLP
                     ETLEPLTEHTHTDREKLIAELHQIREQGYAVDREENTLGLRCFGVAVPYRTPARDAISC
                     SVPVARLTPAHEQLIKDALFDARDRLTLATRRL"
     gene            1770..3305
                     /locus_tag="CHF15_RS03545"
                     /old_locus_tag="SAMN05216252_101709"
     CDS             1770..3305
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718910.1"
                     /locus_tag="CHF15_RS03545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216252_101709"
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="WP_179279639.1"
                     /sec_met_domain="Aldedh (E-value: 2.2e-40, bitscore: 134.1,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVPVWSVDPRTGKQREQVAVEATAEEVDAAVRAAAAAFPALADR
                     EVRARLLRTAADFLEEAGPRILEAADAETALGPARLTGELGRTTYQLRAFAEIVEEGAF
                     LDVIIDHADATLAPPRPDLRRYKIPLGVVAVYSASNFPLAFSVPGGDTASALAAGCPVV
                     VKAHPDHPATSELCADALRRAAVEVGLPADVVSVVHGFQAGIDLVKHPLVSAAGFTGSV
                     RGGRALFDAAAARPVPIPFHGELGSLNPVVITQAAAEERAEQIGAGLAGSMTLGVGQFC
                     VKPGLVLAPAGEAGDRLLKSLAAAVGDVAPGTLLDARMRDNFIAGVRELTTLPEVEAPV
                     EPGDGGEHLVRAGFVSVPAARLAEGGEHDALLEEHFGPVTVVARYGTAEEVEGVLGRLP
                     GNLTATAHLSEAEASGGDGDASGAELLRRLTTLAGRVVVNGWPTGVAVAPAMHHGGPYP
                     ATTSTSTSVGGTAIERWLRPVVYQDAPEGLLPAELRDGNPLGLPRRFNGRREV"
     gene            complement(4101..4994)
                     /gene="thpD"
                     /locus_tag="CHF15_RS03550"
                     /old_locus_tag="SAMN05216252_101710"
     CDS             complement(4101..4994)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="CHF15_RS03550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216252_101710"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_089222064.1"
                     /sec_met_domain="PhyH (E-value: 1.1e-39, bitscore: 132.5,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTATTTDLYPTRGTAEVLTPRQDPVVWGAPGTTGPLRPSELGDF
                     DRDGFLPVDALIGPEDVARYHAELERLCADPAVRADERSIVEAKSRDIRSVFEVHKISP
                     VFAELVRDPRVVDRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPRM
                     RAVSVSIALTENHDTNGGLMIMPGSHRTFLGCAGATPEDNYKKSLQMQDAGTPSHTALT
                     ELAGRHGIRLFTGQPGSAVWFDCNCMHGSGDNITPYPRSNVFIVFNSVENAAVEPFAAP
                     VRRPEFIGARDFTPVR"
     gene            complement(5001..5399)
                     /locus_tag="CHF15_RS03555"
                     /old_locus_tag="SAMN05216252_101711"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045195.1"
                     /locus_tag="CHF15_RS03555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216252_101711"
                     /product="ectoine synthase"
                     /protein_id="WP_089222065.1"
                     /sec_met_domain="ectoine_synt (E-value: 9.1e-55, bitscore:
                     179.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLKDIEGTDRDVTAETGTWRSKRIILADDRVGFSLHETVMY
                     AGTETSMWYANHVEAVLCVEGEGELTDDETGERHRISPGTLYLLDGHERHTMRPQTDLR
                     FVCVFNPPVTGREVHDENGVYPLLTEGA"
     gene            complement(5419..6690)
                     /gene="ectB"
                     /locus_tag="CHF15_RS03560"
                     /old_locus_tag="SAMN05216252_101712"
     CDS             complement(5419..6690)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 3.5e-71.
                     Score: 231.9. Matches aSDomain:
                     nrpspksdomains_CHF15_RS03560_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="CHF15_RS03560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216252_101712"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_089222066.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.3e-74, bitscore:
                     246.9, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPATSVFETLESEVRSYCRGWPAVFDRAQGSRVFNEDGHEY
                     LDFFSGAGALNYGHNNPVLKRALLDYLERDGITHSLDMSTTAKRAFLERFQDVVLRPRG
                     MDHKVMFPGPTGTNAVEAALKLARKVKGRESVVSFTNAFHGMSLGALAVTGNSMKRAGA
                     GVPLVHGTPMPFDNYLDGRVPDFLWFERLLEDQGSGLNQPAAVIVETIQGEGGINVARP
                     EWLRGLADLCRRRDMLLIVDDIQMGCGRTGPFFSFEEAGIVPDIVTLSKSIGGYGMPMS
                     LTLFRPELDIWEPGEHNGTFRGHNPAFVTATAALDTYWADGASMEKQTLKRGEQAEAVL
                     REICAEHPRSGARYRGRGLVWGLEFTDPGRASRVCARAFELGLLLETSGPRGEVVKLLP
                     ALTITPEELDEGLRTLARSVRETA"
     aSDomain        complement(5593..6606)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS03560_Aminotran_3.1"
                     /evalue="3.50E-71"
                     /label="CHF15_RS03560_Aminotran_3.1"
                     /locus_tag="CHF15_RS03560"
                     /protein_end="366"
                     /protein_start="28"
                     /score="231.9"
                     /tool="antismash"
                     /translation="FDRAQGSRVFNEDGHEYLDFFSGAGALNYGHNNPVLKRALLDYLE
                     RDGITHSLDMSTTAKRAFLERFQDVVLRPRGMDHKVMFPGPTGTNAVEAALKLARKVKG
                     RESVVSFTNAFHGMSLGALAVTGNSMKRAGAGVPLVHGTPMPFDNYLDGRVPDFLWFER
                     LLEDQGSGLNQPAAVIVETIQGEGGINVARPEWLRGLADLCRRRDMLLIVDDIQMGCGR
                     TGPFFSFEEAGIVPDIVTLSKSIGGYGMPMSLTLFRPELDIWEPGEHNGTFRGHNPAFV
                     TATAALDTYWADGASMEKQTLKRGEQAEAVLREICAEHPRSGARYRGRGLVWGLEFT"
     gene            complement(6739..7233)
                     /gene="ectA"
                     /locus_tag="CHF15_RS03565"
                     /old_locus_tag="SAMN05216252_101713"
     CDS             complement(6739..7233)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545812.1"
                     /locus_tag="CHF15_RS03565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216252_101713"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_089222067.1"
                     /transl_table=11
                     /translation="MTVARELLLDTPGIEDGAALWRIARDSRTLDLNSPYSYLLWCRDF
                     AGTSVVARTAHGEPIGFVTGYVRPERPRTLVVWQVAVDEAHRGRGLAAAMLDDLAQRAA
                     ASGVREVETTITPDNEASHRLFTSFAARHGARTERGVLFDTALFPDGHGPEVLYRIGPL
                     V"
     misc_feature    complement(7339..7354)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.35"
                     /tool="antismash"
     misc_feature    complement(7374..7389)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7487..7500
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.8"
                     /tool="antismash"
     misc_feature    complement(7487..7502)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.45"
                     /tool="antismash"
     gene            complement(7605..8690)
                     /locus_tag="CHF15_RS03570"
                     /old_locus_tag="SAMN05216252_101714"
     CDS             complement(7605..8690)
                     /EC_number="2.6.1.-"
                     /NRPS_PKS="Domain: Aminotran_5 (14-292). E-value: 1e-31.
                     Score: 102.1. Matches aSDomain:
                     nrpspksdomains_CHF15_RS03570_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560636.1"
                     /locus_tag="CHF15_RS03570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216252_101714"
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="WP_089222068.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.4e-25, bitscore:
                     85.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSHPLLDLPPLTAERFAAIERRVAALLHTEQDVVITQGEALLPLE
                     GCIRGGARPGSTALNVVTGPYGQTFGNWLRDAGATVVDLEVPFHAAVSAQDVARALSGH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRSHGALFMVDAVASVAAEPLLVDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWRRLADNPAAPRRSYLSLLDWKERWIDAGRKALPHAP
                     AQLEMLALEACLERIEEAGLDAVIARHRAAAAATRAGAAALAAVEPYVYRDEDAAPVAT
                     TLRTLDGVAADGLVARALALEPGLPLVAGGGALAKEMIRVNHYGRDADPAVVVRTLRTL
                     AGVLEADPEPAVAAADAAWQV"
     aSDomain        complement(7815..8648)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHF15_RS03570_Aminotran_5.1"
                     /evalue="1.00E-31"
                     /label="CHF15_RS03570_Aminotran_5.1"
                     /locus_tag="CHF15_RS03570"
                     /protein_end="292"
                     /protein_start="14"
                     /score="102.1"
                     /tool="antismash"
                     /translation="RFAAIERRVAALLHTEQDVVITQGEALLPLEGCIRGGARPGSTAL
                     NVVTGPYGQTFGNWLRDAGATVVDLEVPFHAAVSAQDVARALSGHPEIDFVSLVHAEAA
                     TGNTNPVAEIGEVVRSHGALFMVDAVASVAAEPLLVDAWGVDLCVIGAQKAMGGPAGVS
                     AVSVSERAWRRLADNPAAPRRSYLSLLDWKERWIDAGRKALPHAPAQLEMLALEACLER
                     IEEAGLDAVIARHRAAAAATRAGAAALAAVEPYVYRDEDAAPVATTLRTLDGVAAD"
     gene            8759..9859
                     /locus_tag="CHF15_RS03575"
                     /old_locus_tag="SAMN05216252_101715"
     CDS             8759..9859
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560635.1"
                     /locus_tag="CHF15_RS03575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216252_101715"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_089222069.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.2e-20, bitscore:
                     69.6, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADREAPQALHVKGRILAGPQDVRDELWVVGGRVTYERPAGPRDL
                     TTVEGWVLPGLVDAHCHVGLDHHGPVDADTSEKQALTDRDAGTLLLRDAGSPSDTRWID
                     DREDLPRIIRAGRHIARTRRYIRNYAWEIEPEDLVAYVRREARRGDGWVKLVGDWIDRD
                     AGDLTACWPRGAVEAAIAAAHEEGARVTAHCFAEDSLRDLVEAGIDCVEHATGLTEDTI
                     PLFAAHGVAIVPTLVNIATFPELADGGQARFPRWADHMRRLHARRYDTVRAAHDAGIRI
                     FTGTDAGGSLAHGLVAAEVAELVTAGLPALDALSAATWSAREWLGRDGLTEGASADLVV
                     YPEDPRADVRVLTAPRRIVLRGRVVG"
ORIGIN
        1 cgggctgggc gccgacatcg ccgccctcgc cgaggtcgcc ggcacccgga ccgtcgcgga
       61 cgccgccgtg ctgtgggact ggcagtcctg gtgggcgcag aagctggagt ggcggcccag
      121 ccaggaccac gacgcgcgcg aacgcgcgga cagctggtac gaggccctgt tcgaccgcca
      181 cctgaccgtc gacttcgccc gcccggaagc cgatctgtcg gcctatcccc ttgtcgtcgt
      241 ccccgccctc tacctcacca ccgaggccgc cggacggaat ctgcgcgcct acgtcgaggc
      301 cggcggcacg ctgctggtct cgtacttctc aggcgtcgtc gacgagcacg acaccgtcca
      361 tccgggcccc gtgcccggcg cgctgcggga cgttctcggg ctgaccgtcg aggagttcgc
      421 gccgctgctc gacggcgaac gggtccggct cgacggcccc gaggctcccg cgctcaccgg
      481 cgacctgtgg acggaggtcg tcgtaccgcg cggcgccgag ccggtgtgga cgtacgccga
      541 cggccccgcc gcaggccgcc ccgccgtcac ccggcacgcc ctgggggccg gcacggcctg
      601 gtacgtctcc acccgtctcg gccccgcgga gctcggcgcg gtcctggccg ccgcggcgca
      661 ggacacgggc ctgccggacc gggcggaact gccgcatgac gtcgaggtcg tccgccgcgc
      721 cggggagcag ggcgcgtacc tcttcgtgat caaccacagc ggggccgagg caaaggtgcc
      781 gctcgacgtg ccgggcacgg aactgctcac cggggagcgg gccatcggcc ggctcagcgt
      841 gccgccgggc gcggtccggg tgctccggga ggagtcctga gcaggcggat cagaggcggc
      901 gggtcgccag cgtgaggcgg tcgcgcgcgt cgaagagggc gtccttgatc agctgctcgt
      961 gcgccggggt cagccgggcc accggcaccg agcagctgat cgcgtcccgg gcgggcgtgc
     1021 ggtacgggac ggccacgccg aagcagcgca ggccgagcgt gttctcctcg cggtccacgg
     1081 cgtaaccctg ctcgcggatc tggtgcagct cggcgatcag cttctcgcgg tcggtgtgtg
     1141 tgtgctcagt gagcggctcc agcgtctccg gcagcagctt gcggacctgc tcgtcggtgt
     1201 acgtggcgag cagcgccttg ccgagcgagg tggagtgggc cggcagccgg cggccgacgc
     1261 gggtgaacgg ccgcaggtag tgcgaggact gacgggtcgc caggtacacg acgctggtgc
     1321 cgtcgaggcg ggcgaggtgg atggtctcgc tggtgtcgtc cgagagccgg tccagggtcg
     1381 ggcgggccgc ggccaccacc tcgtcgccgt cgatgtacga ggtgccgacc agcagggcgc
     1441 gcacgccgat cccgtaacgt gtgccggtgg cgtcggtctc gatccagccc agctccacga
     1501 gcgtgcgcag cagcatgtac aggctggact tggggtagcc gacggcctcc tgcacggcgg
     1561 ccaggctgtg catgccgggc tgcccggcga agaactccag cagctccacc gtccgcaccg
     1621 cggatttgac ctgggccccg ccctcacctg cagccatctg gagtggaccc ctcttgttcg
     1681 gccgggaccc gcgcaagtag gctcccgaac agattcacag ttgcggatgc tgttcagcat
     1741 atcgaacgaa aggcctggag gtacccgcgg tggccgtacc agtgtggagc gtcgatcccc
     1801 gaaccgggaa gcagcgggag caggtcgccg tcgaagccac cgccgaggag gtcgacgccg
     1861 ccgtacgcgc cgccgccgcg gccttcccgg cgctcgccga ccgcgaggtg cgggcccggc
     1921 tgctgcgcac cgcggccgac ttcctcgagg aggccggccc ccgcatcctg gaggccgccg
     1981 acgcggagac cgccctcggt cccgcccgcc tcaccggcga actcgggcgc accacctacc
     2041 agctgagggc cttcgccgag atcgtcgagg agggcgcctt cctcgacgtc atcatcgacc
     2101 acgccgacgc gacgctcgcc ccgccccggc ccgacctgcg gcgctacaag atcccgctcg
     2161 gcgtcgtggc cgtctactcc gccagcaact tcccgctcgc gttctccgtg ccgggcggcg
     2221 acaccgccag cgcgctcgcg gcgggctgcc ccgtcgtcgt caaggcccac cccgaccacc
     2281 cggcgacgag cgagctctgc gccgacgcgc tgcgccgcgc cgccgtcgag gtcggcctgc
     2341 ccgccgacgt cgtctcggtc gtccacggct tccaggccgg catcgacctc gtcaagcacc
     2401 cgctggtctc cgccgccggg ttcaccggct ccgtacgggg cgggcgcgcc ctcttcgacg
     2461 cggcggcggc acgacccgtg ccgatcccgt tccacggcga actcggcagc ctcaacccgg
     2521 tcgtcatcac gcaggccgcg gccgaggagc gcgccgagca gatcggcgcg gggctcgccg
     2581 ggtcgatgac gctcggcgtc ggccagttct gcgtcaagcc cggcctggta ctggccccgg
     2641 cgggggaggc gggcgaccgc ctcctcaagt ccctggcggc cgcggtgggc gacgtcgccc
     2701 ccggcaccct gctcgacgcc cgcatgcggg acaacttcat cgcgggtgtg cgcgaactga
     2761 ccacgctgcc cgaggtggag gcgccggtcg agcccggcga cggcggcgag cacctggtgc
     2821 gcgccggctt cgtctccgtc ccggccgccc ggctcgcgga gggcggcgag cacgacgcgc
     2881 tgctggagga gcacttcggg cccgtcacgg tcgtcgcgcg gtacggcacc gcggaggagg
     2941 tcgagggggt gctcggacgg ctccccggga atctcacggc gacggcgcac ctgagcgagg
     3001 ccgaggcgtc cggcggcgac ggggacgcct ccggcgcgga actgctgcgc cggctcacca
     3061 cgctggcggg gcgcgtcgtg gtgaacggct ggcccacggg cgtcgcggtg gccccggcca
     3121 tgcaccacgg ggggccgtac ccggccacga cctcgacgtc cacgtcggtc ggcggcacgg
     3181 cgatcgagcg gtggctgcgg cctgtcgtgt accaggacgc gccggagggg ttgctgccgg
     3241 cggagctgcg tgacggcaac cccttggggt tgccgcgccg gttcaacggg cgccgcgaag
     3301 tctgaggctt gcgccttctg cgccgggtgg gttgtggttg cggccggtgg ttgtgtgcgg
     3361 gttgtttgcg cctgcgggcg gctgcgccgg gctgcccggt gggtgtgtgc gggttgtttg
     3421 cgcctgcggc gggcggttct cccgccctcc cgcccgattg cccggcgcgg tcagtgcggg
     3481 cctcgcaccg tgtgcctggg tcggtgccgc gccggcgcac gtcctcaacg ctcgcggttt
     3541 acgcccacat cgttcggcac cgggtccgcc gctcgtcgtt tccgggcgca ccccgccaca
     3601 ccccctccgg tcgtgtgggc gactgacggc cggagggggg taggggcggt ccggggttgc
     3661 cgcacgcgtc ttgctgaacc cccatcggcc gccagcggcc accagtgcgc gcaccctgtt
     3721 tcggcctgcg gccgggggac ctcccgctgg ggcagcatcc agtggtgtgg ggaggggcgc
     3781 cctcccatca cccccctccg gccgttggtc gctcatcgat acggaggggg cggggtcgta
     3841 ggggtgggcg ttctggacgc tgacgtgtat ttgttgtgcg gcacagacgg caccgacctg
     3901 gaccgcccgc catgcacata aatatgcggt acagaatgcc cagccccgga ggccccgccc
     3961 ccggcccgcc acgaaccgcg cgaggcccgc accaatcatc cggggcaatc gggcgggcgg
     4021 gcgggcgaac ccccaaccgc aaccacgacg cactcgcaca caacaacgca ccggacacag
     4081 agccggcgcc ccccggaggg ttaccgcacg ggcgtgaagt cgcgcgcgcc gatgaactcg
     4141 ggccgccgca caggcgccgc gaacggctcg acggccgcgt tctccacgct gttgaagacg
     4201 atgaagacgt tgctgcgcgg gtacggcgtg atgttgtccc ccgagccgtg catgcagttg
     4261 cagtcgaacc agaccgcgga ccctggctgc ccggtgaaga gccggatccc gtgccggccc
     4321 gccagttccg tgagggccgt gtgggagggc gtgcccgcgt cctgcatctg gagggacttc
     4381 ttgtagttgt cctccggcgt cgcgcccgcg cagcccagga acgtccggtg cgagcccggc
     4441 atgatcatca ggccgccgtt ggtgtcgtgg ttctcggtca gcgcgatgga gaccgagacc
     4501 gcgcgcatgc ggggcagccc gtcctcggcg tgccaggtct cgaagtcgga gtgccagtag
     4561 aagccggagg cgccgaagcc cggcttgacg ttgatccggc tctggtggac gtagacgtcg
     4621 gagccgagga tctgccgggc acggtcgacg acgcgggggt cgcggaccag ttcggcgaag
     4681 accgggctga tcttgtggac ctcgaagacc gagcggatgt cccgggattt cgcctccacg
     4741 atggaccgct cgtccgcgcg cacggccggg tcggcgcaca gccgctccag ctcggcgtgg
     4801 tagcgggcca cgtcctcggg cccgatgagc gcgtcgacgg gcaggaagcc gtcgcggtcg
     4861 aagtcgccga gttcggacgg ccggagcggg ccggtcgtgc cgggtgcgcc ccagacgacc
     4921 gggtcctggc gcggggtcag cacctcggcg gtgccgcggg tggggtagag gtcggtcgtc
     4981 gtcgcggtgg tcatcgctgg tcacgctccc tcggtgagca gggggtagac gccgttctcg
     5041 tcgtgcacct cgcgcccggt gacgggcggg ttgaagacgc agacgaagcg caggtcggtc
     5101 tgggggcgca tggtgtgccg ttcgtggccg tcgagcaggt agagcgtgcc gggggagatc
     5161 cggtgcctct cgccggtctc gtcgtcggtc agctcgccct cgccctcgac gcacagcacc
     5221 gcctcgacgt ggttggcgta ccacatggag gtctcggtcc ccgcgtacat cacggtctcg
     5281 tgcagtgaga agcccacgcg gtcgtcggcg aggatgatgc gcttgctgcg ccaggtgccg
     5341 gtctcggcgg tgacatcgcg gtcggtgccc tcgatgtcct tcagggatcg cacgatcacg
     5401 gtgggacggt tcctttcgtc aggcggtctc gcggacggag cgggcgaggg tgcgcagccc
     5461 ctcgtcgagc tcctcggggg tgatggtcag ggcgggcagc agtttgacga cctcgccgcg
     5521 ggggccggag gtctccagca gcaggcccag ttcgaaggcg cgggcgcaga cccgggaggc
     5581 ccggccgggg tcggtgaact ccaggcccca caccaggccg cggccccggt accgggcgcc
     5641 ggagcgcgga tgctcggcgc agatctcgcg caggacggcc tcggcctgct ctccgcgctt
     5701 gagtgtctgc ttctccatgc tcgcgccgtc cgcccagtag gtgtcgagcg cggcggtggc
     5761 ggtgacgaag gcggggttgt ggccgcggaa ggtgccgttg tgctcgcccg gctcccagat
     5821 gtccagttcg ggacggaaga gggtcagcga catcggcatg ccgtagccgc cgatcgactt
     5881 ggagagggtc acgatgtccg gcacgatgcc cgcctcctcg aaggagaaga acgggccggt
     5941 gcggccgcag cccatctgga tgtcgtcgac gatcagcagc atgtcgcggc gccggcacag
     6001 gtcggccaga ccgcgcagcc actcggggcg ggcgacgttg atgccgccct ctccctggat
     6061 cgtctcgacg atcaccgcgg cgggctggtt gagaccggac ccctggtcct ccagcagccg
     6121 ctcgaaccac aggaagtccg ggacgcggcc gtcgaggtag ttgtcgaagg gcatcggggt
     6181 gccgtgcacc agggggacgc cggcgccggc ccgcttcatc gagttgccgg tgacggcgag
     6241 cgcgcccagc gacatgccgt ggaaggcgtt ggtgaaggac accaccgact cacggccctt
     6301 gaccttgcgg gccagcttca gcgccgcctc gacggcgttg gtgccggtcg gtcccgggaa
     6361 catcaccttg tggtccatgc cgcgcgggcg cagcacgacg tcctggaagc gttccaggaa
     6421 ggcgcgcttg gcggtggtgc tcatgtccag gctgtgcgtg atgccgtcgc gctccaggta
     6481 gtcgagcagg gcgcgtttga gcacggggtt gttgtgcccg tagttgaggg cgccggcgcc
     6541 ggagaagaag tcgaggtact cgtggccgtc ctcgttgaag acgcggctgc cctgcgcgcg
     6601 gtcgaagacg gcgggccagc cgcggcagta gctgcggacc tcggactcga gggtctcgaa
     6661 gacggacgtg gcgggcgggg tgatggtcac cgtgcattcc ttctgtgggg gaggaggagt
     6721 cgggcgggga cggccgggtc agacgagggg cccgatgcgg tacaggactt cgggtccgtg
     6781 gccgtcgggg aacagcgccg tgtcgaagag caccccgcgc tcggtccgtg ctccgtggcg
     6841 ggcggcgaag gacgtgaaca ggcggtgcga ggcctcgttg tccggggtga tcgtggtctc
     6901 gacctcgcgg acgccggacg cggcggcccg ttgtgcgagg tcgtcgagca tcgcggccgc
     6961 gagcccccgg ccgcggtgcg cctcgtccac ggcgacctgc cagacgacga gggtgcgggg
     7021 gcgttcgggg cggacgtagc cggtcacgaa gccgatcggt tcgccgtgcg ccgtacgcgc
     7081 caccacggag gtgccggcga agtcccggca ccacaggaga tagctgtacg gggagttcag
     7141 gtccagggtc cgtgaatcac gggcgatgcg ccacagtgcg gcgccgtcct cgattcccgg
     7201 ggtgtcgagc agcaattccc tagcgacggt catgcggaga caacgtagcg ggcggaattc
     7261 cgaatcgcat gccgcgcggc ccttgtggtg ggtggatgcc gctgatatca ggcgcgcgga
     7321 ggccgcggag atgggtttgt ttacccgtat atttcggggc agaagggtgg cattgtggcc
     7381 tcgatcacat cctcgaaatc atcacctggt gtcgatgaaa tcttggcgtt tgaagccgtg
     7441 atcccggggc aggagttgaa tgcggaatac cttccgggat gccccggata attactcgga
     7501 atttccccgc cgtcaagtcg gcatggaatt cggcgcgccg aaacacaagg gccgccccag
     7561 ccgttcgcga cggccggggc ggcccctggg ggaatgcggt tctctcagac ctgccatgcg
     7621 gcatcggccg ccgccacggc cggctcgggg tccgcctcca ggacgccggc cagcgtgcgc
     7681 agcgtacgga cgaccacggc ggggtcggcg tcccggccgt agtggttgac ccggatcatc
     7741 tccttggcca gtgccccgcc gccggcgacc agcgggagcc ccggttccag ggccagggcg
     7801 cgggcgacca acccgtcggc ggcgacgccg tccagggtgc gcagcgtcgt cgcgaccggc
     7861 gcggcgtcct cgtcccggta cacgtacggc tcgacggcgg ccagcgccgc cgcaccggcc
     7921 cgggtcgccg ccgcggcggc acggtgccgg gcgatcaccg cgtcgagccc ggcctcctcg
     7981 atccgctcca ggcaggcctc cagcgccagc atctccaact gcgcgggagc gtgcggcagc
     8041 gccttgcgcc cagcgtcgat ccagcgctcc ttccagtcca gcagcgacag gtacgaacgg
     8101 cgcggcgccg ccgggttgtc cgccaggcgc cgccacgcgc gctcgctcac cgacaccgcc
     8161 gacaccccgg cgggcccgcc catcgccttc tgggcgccga tcacacacag gtccacgccc
     8221 cacgcgtcca ccagcagcgg ctcggccgcc accgacgcga ccgcgtcgac catgaacagc
     8281 gcgccgtgcg accggaccac ctcgccgatc tccgccaccg ggttggtgtt gccggtcgcc
     8341 gcctccgcgt gcaccagcga cacgaagtcg atctccgggt ggccggacaa cgcccgcgcg
     8401 acgtcctgcg cgctcacggc ggcgtggaag ggcacctcca ggtcgaccac ggtggcgccc
     8461 gcgtcccgca gccagttgcc gaaggtctgg ccgtacggtc cggtcaccac gttcagcgcc
     8521 gtcgaacccg gccgcgcccc gccgcggatg cagccctcca gcggcagcag ggcctcgccc
     8581 tgcgtgatca cgacgtcctg ctccgtgtgc agcagcgcgg cgactctccg ctcgatcgcg
     8641 gcgaagcgct cggcggtcag cgggggcagg tcgagcaggg ggtgggacac ggcggcgctg
     8701 cttccttcgt cggggggtgt acggcccgag ggtatccggc ggctcgtaga gtgcagacat
     8761 ggctgatcgc gaggcgccgc aggcactgca cgtcaagggc cgcatcctgg ccgggccgca
     8821 ggacgtacgg gacgagctct gggtggtcgg cgggcgcgtc acctacgagc gccccgccgg
     8881 tccgcgagac ctcaccaccg tcgagggctg ggtcctgccg ggcctggtcg acgcccactg
     8941 ccacgtcggc ctcgaccacc acggccccgt cgacgcggac acctccgaga agcaggcgct
     9001 caccgaccgc gacgcgggca ccctgctgct gcgcgacgcc ggttccccct ccgacacccg
     9061 ctggatcgac gaccgcgagg acctgccgcg gatcatccgg gccggccggc acatcgcccg
     9121 tacccgccgc tacatccgca actacgcctg ggagatcgag cccgaggacc tggtcgccta
     9181 cgtccggcgg gaggcccggc gcggggacgg ctgggtcaag ctggtcgggg actggatcga
     9241 ccgcgacgcc ggcgacctca ccgcctgctg gccgcgcggt gccgtcgagg ccgccatcgc
     9301 cgccgcccac gaggagggcg cccgcgtcac cgcccactgc ttcgccgagg actccctgcg
     9361 ggacctggtc gaggcgggca tcgactgcgt cgagcacgcc accgggctca ccgaggacac
     9421 catcccgctc ttcgccgcgc acggggtggc catcgtcccc accctggtca acatcgcgac
     9481 cttcccggaa ctcgcggacg gcggccaggc caggttcccg cgctgggcgg accacatgcg
     9541 ccggctccac gcccgccgct acgacaccgt ccgcgcggcc cacgacgccg gcatccggat
     9601 cttcaccggc accgacgccg gcggctccct cgcccacggg ctcgtcgccg cggaggtcgc
     9661 cgaactggtc accgccggtc tgcccgccct cgacgcgctc tccgccgcca cctggtcggc
     9721 ccgcgagtgg ctcggccgcg acggcctcac cgagggggcc tccgccgatc tggtcgtcta
     9781 tccggaggac ccccgggccg acgtccgcgt cctgaccgcg ccccggcgca tcgtgctgcg
     9841 cggccgcgtg gtggggtgac ccggcggaca ccgcggagcc acggtagcgg tgcgccgccg
     9901 gcggtgcgcc cggaatgatc ccggagcacc gattcgggtg atcctgtacg ggaacagtcg
     9961 atccgtccct ttccttcgtg ctgcgatcgg tgcagccggc cgacaccgca acgggacggg
    10021 gagcaccatg accaggcagg gaccgcagaa gtacccgggc gcctcgcgaa ggtactggta
    10081 ccaggacgac tacggcggcg acgcgatgga ggtcaacgcc ctcgtcctgc acaccaccga
    10141 gggccgcagc gtgccgacgt acgggggcgg cggcagcgcg cccaacctga cggcgaaacc
    10201 ggacttcgag gacgagaagc tggtctggta ccagcacttc gacatcgaca tctcctcgcg
    10261 cgccctggtc aaccggcccg gcggggtgga gaccaacacc ctcaacgtgt gccaggtgga
    10321 gctggtgggc acctgcgatc ccgagcggca cgaggactgg ggcgccgcca accggcacat
    10381 ctactggccc aaggccccc
//
