LOCUS       NZ_LT906439            10297 bp    DNA     circular CON 15-DEC-2024
DEFINITION  Streptococcus merionis strain NCTC13788 chromosome 1, complete
            sequence.
ACCESSION   NZ_LT906439
VERSION     NZ_LT906439.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus merionis
  ORGANISM  Streptococcus merionis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2017) WTSI, Pathogen Informatics, Wellcome Trust
            Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900187085.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 13:40:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,228
            CDSs (total)                      :: 2,160
            Genes (coding)                    :: 2,111
            CDSs (with protein)               :: 2,111
            Genes (RNA)                       :: 68
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 27 of 49
            Pseudo Genes (incomplete)         :: 28 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 16 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:09:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 535129
            Orig. end                         :: 545426
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT906439.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10297
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10297
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5297](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5297
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            379..2616
                     /locus_tag="CKV83_RS02445"
                     /old_locus_tag="SAMEA4412692_00493"
     CDS             379..2616
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002904090.1"
                     /locus_tag="CKV83_RS02445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00493"
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="WP_018373725.1"
                     /transl_table=11
                     /translation="MLRWIRTTALPPIQLALMLIVLFYTLRFPSPITLFLLGFLCWRLF
                     WKHSRAVIQASLMILVPFGFFFVAQRYLAAQANHSAPESISQLQMIPDSIAVNGDRLSF
                     RAKEAGQTYQFFYKLKTQEEQAFFQKLSQPVRLTVEAEVSEAESQRNFNGFDYQAYLET
                     QGIYKTVQVKTISEIVPVHSWNVFDLLSKWRRSALVYIHQQFPNPMRHYMSGLLFGHLD
                     KDFDQMGDLYSGLGIIHLFALSGMQVGFFVDKFRWLLLRLGLTQETVDWLQLPFSLFYA
                     GMTGFSASVIRSLIQKNLANQGFSGLNNLGLTVLISFVLMPNFLLTAGGVLSFGYAFIL
                     TMQDFEHLTGIKRALSEAFALSLAILPVLIWYFATFQPLSILLTFAFSLLFDVLFLPLL
                     SLVFLLSPLWKIAELNSLFVGLEGIIRWVAGLASRPLVFGKPSLLVLVFLLISLGLLYD
                     FWQHKKARVGLGVLIIGLFLITKCPLTNEVTVVDIGQGDSIFLRDMKGRNVLIDVGGKV
                     SFSGKEAWQQAQSDSNAERTLIPYLKSRGVSKIDTLVLTHTDTDHIGDLLPVAKNFDIG
                     QIWVSSGSLTKADFVKTLESLGVPVHVTKVGDQIPICDSYLEVLYPREIGDGGNNDSIV
                     LYGQLLQTRFLFTGDLEHGELDLIKAYPNLRVDVLKAGHHGSKGSSYPEFLDHIKPQIG
                     LFSAGKNNRYKHPHPETVARFDERNITTYRTDQQGAIRFHGWKTWQVETVR"
     gene            2878..3750
                     /locus_tag="CKV83_RS02450"
                     /old_locus_tag="SAMEA4412692_00494"
     CDS             2878..3750
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940091.1"
                     /locus_tag="CKV83_RS02450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00494"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_026216957.1"
                     /transl_table=11
                     /translation="MGKLLASRMRSRRKELNLSQVELAAGICEQAQISKIERDADYSPG
                     VDLIYALAKKLNVTMDYFFDENIHVESDYLTQFRAVSKKFLDLRDYKSLKYIYELEAAK
                     TVKLPLSDQLYLQWIEAVVLFNHDHKQSEAIKKLESILSKYSEYDWEYLNISNSLLHFY
                     FQTDERSKFEEVYNHMTNLFKSVKVRTIDELEILIKCRYNFCRYLWLNHQTETAISETL
                     ETIDICLKNNSFYCLANLYCLLGNVSEDFAHKDVVKQYFVTAQHVYQLERNDKMALELE
                     RFINETFPA"
     gene            3870..4343
                     /gene="tnpA"
                     /locus_tag="CKV83_RS02455"
                     /old_locus_tag="SAMEA4412692_00495"
     CDS             3870..4343
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="tnpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373723.1"
                     /locus_tag="CKV83_RS02455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00495"
                     /product="IS200/IS605 family transposase"
                     /protein_id="WP_018373723.1"
                     /transl_table=11
                     /translation="MAKTSYSLSHTKWMCKYHIVFTPKYRRKSIYYKIRQDLIEIFRHL
                     CKYKGVEIIEGHMMPDHVHMLVLIPPKLSISDFMGYLKSKSALMIFDKHANLKYKYGNR
                     KFWARGYYVSTVGLNEKTVAKDIREQEKDDIALDRLSVKEYEDPFSDGGFRTR"
     gene            4588..4725
                     /locus_tag="CKV83_RS11385"
     CDS             4588..4725
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CKV83_RS11385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_018373722.1"
                     /transl_table=11
                     /translation="MKKATKKFIIAILLVGLLIIIPKPSGTPTPTPLGFDPGELYNIGV
                     "
     misc_feature    4828..4843
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 20.83"
                     /tool="antismash"
     misc_feature    4828..4844
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 23.21"
                     /tool="antismash"
     gene            5001..5297
                     /locus_tag="CKV83_RS02460"
                     /old_locus_tag="SAMEA4412692_00496"
     CDS             5001..5297
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /locus_tag="CKV83_RS02460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00496"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_018373721.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.2e-06,
                     bitscore: 26.1, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKIRTVIATALTILMFSVALPVLAVSHRGGEWNYGGHHDPDNWG
                     AFSNYYHATNYHWSYVGSTGRANERTVYAGARSAAHAFINTNFGEHVNFDAGW"
     gene            5399..7417
                     /locus_tag="CKV83_RS02465"
     CDS             5399..7417
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514778.1"
                     /locus_tag="CKV83_RS02465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_018373720.1"
                     /transl_table=11
                     /translation="MKRSFVVLSNLLVTLFFVWVHTIWEDTYVSHYYPNISIFSSSPEA
                     TFDKVADSLSKLAEETDSLIALQHQDPDSEGKPAFSYTVFGQGELPENLSEKSLEAAAN
                     GSLVTNYFIFRGKLSVERLKEELSRVGLTKPFVMKPSSLKILALVFTPGFQFLALLIFL
                     LTFGALSLISQILNLRTAGIRLISGENRWKIFLRPLSQDIIHTAIGLIVGFLFAVVLKQ
                     FVSFPLVTYQTIGTGLVVYNLCLLLITLFFAGLFAIGIKKVPLMQVIKGQVPVRGIISL
                     ILIGQLLAVIIVSIGVSRTSIYSKAWQQQEQGREAWRQESNLVALSMARGGADRRQGQE
                     ELAREQKVWFNLVGQAVSEQGGLLSQHNLADRAWQNGVSSSKNFSTSTEWQDYSPQGNV
                     LIVTPQYLKQQQIAVAPEIEEKVAHLATGEFVLLLPEHLRSEESHYKNIFEEDISHRMS
                     SQDTRQEMVATVSYLESGHDRFVYNTTPISYQQLLRDPIIVVLTPQSTGEQSFSFWEQS
                     VSNFFFFENLKNAQALIEKYGIENWVAELKPAYQIHQTLLDNLKREMWTMAAGAILGTL
                     TSILMFNTMNQLYFEEFRRDIFIKRISGLRFWEIHKKYLLAQLFVFFLGFLVSIFLATD
                     ILIAFLVLLLFIGNAILQLHFQMHKENKMSTLILKGA"
     gene            7419..8048
                     /locus_tag="CKV83_RS02470"
                     /old_locus_tag="SAMEA4412692_00498"
     CDS             7419..8048
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373719.1"
                     /locus_tag="CKV83_RS02470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00498"
                     /product="putative bacteriocin export ABC transporter"
                     /protein_id="WP_018373719.1"
                     /transl_table=11
                     /translation="MIRLEKVSKQFGERFLFSDLSMTFEAGKVYALIGSSGSGKTTLMN
                     MMAKLETYDGNIFFRNKNLNQFKSSEFFRHELGYLFQNFGLIESQTIKENLKLGLIGQK
                     LSKREQQQKEEEALNRVGLSYLNLDKRIFELSGGESQRVALAKVILKNPPFILADEPTA
                     SIDPETAKAIMAILLSLRNENRLIIVATHNPTIWEMADEIISMDRL"
     gene            8284..8610
                     /locus_tag="CKV83_RS11675"
                     /old_locus_tag="SAMEA4412692_00499"
     CDS             8284..8610
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013842.5"
                     /locus_tag="CKV83_RS11675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00499"
                     /product="IS630 transposase-related protein"
                     /protein_id="WP_095114558.1"
                     /transl_table=11
                     /translation="MTYELDFRKRVIAYVQEGHTKKETSKLFGISPNTLYLWEKQLREL
                     NSLECQPRKRKPFKIPLEKLEQFVQQYPDAFLREIAEHFDCSVPSVWTALKKLDITLKK
                     DDEL"
     gene            8600..9127
                     /locus_tag="CKV83_RS11680"
                     /old_locus_tag="SAMEA4412692_00500"
     CDS             8600..9127
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1238: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF024751.5"
                     /locus_tag="CKV83_RS11680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00500"
                     /product="transposase"
                     /protein_id="WP_095114559.1"
                     /transl_table=11
                     /translation="MSFKEQDVHKVQQYLEVLACFSNTPIVYIDETGIDTYLYRKQGRA
                     KRGQKIFDKKSGRRFERTSLVAGQIGNQLIAPMMYKESMTSDFFVAWFEQQLIPSLREP
                     HLVIMDNASFHPKSRLDNIGIQHHHYFLPLPPYSPELNPIEQTWAVLKYHITELLRKLS
                     SISECFEDYFKT"
     misc_feature    9838..9855
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 25.17"
                     /tool="antismash"
ORIGIN
        1 agctggaagc gggtagtcgt ctagcggatg ctgtggcaga agcaggaggt ttgagcgagg
       61 aggcagacag taaatccatt aatctggcac agaaattgag cgacgaagca gtcgtctacg
      121 tagccaagca gggcgaggaa gtcagcgtcg tccaaagctc agcagcaaca ggaacttcaa
      181 gcacttctca atctggtaac gacctagtca atatcaatac tgccacccta gctgaactcc
      241 agaccatctc aggtatcggt gccaagcgcg ctcaggacat catcgattac cgcgaagcca
      301 atggcggctt caaaaccatt gacgatctca acaatgtctc aggtatcggc gacaaaacca
      361 tggagaattt gagagcctat gttacggtgg attagaacga cagctctccc acccatccaa
      421 ctagccttga tgcttattgt acttttctac accctgcgct ttcccagtcc tatcaccttg
      481 tttttgctgg gctttttatg ctggcggctt ttttggaaac atagcagagc agtgattcag
      541 gctagcttga tgattttagt tccctttggc ttcttttttg ttgctcagcg ctatctggcc
      601 gctcaggcca atcattctgc gcctgaaagc attagccaat tgcagatgat cccagacagc
      661 atcgctgtca atggggatcg tctatcgttt agagctaagg aagctggtca gacctatcag
      721 tttttttaca aactcaaaac gcaagaagaa caggccttct ttcaaaagct atctcagcca
      781 gtccgcttga cggtagaagc agaagtgtca gaagctgaga gtcagcgcaa tttcaacggt
      841 tttgactacc aagcctatct ggaaacccaa gggatttaca agacggtgca ggtcaagact
      901 atttctgaga ttgtaccagt tcactcttgg aatgtctttg accttctttc caagtggcgt
      961 cgcagtgctc tagtctatat tcatcagcag tttcccaatc ccatgcgcca ttacatgtca
     1021 ggcctgcttt ttggccattt ggataaggat tttgatcaga tgggagatct ttattccggt
     1081 cttggcatta ttcacctctt tgccctgtct ggcatgcagg ttggcttctt tgtggataag
     1141 tttcgctggc tattgctccg tttgggtcta actcaggaaa cggtggattg gctgcaactc
     1201 cctttttcct tgttttatgc tggtatgact ggtttttcgg cctcggtcat tcgtagtctg
     1261 attcagaaaa acttggctaa tcaagggttt tcaggactta ataaccttgg cttaaccgtc
     1321 cttatcagct ttgtcctcat gcctaatttt ttactaacag cgggtggggt tctcagtttt
     1381 ggctatgcct ttatcctgac catgcaggac tttgagcatc tgacaggcat caagcgcgca
     1441 ctatctgagg cttttgccct ctcacttgct atcctccccg tcctcatctg gtacttcgct
     1501 accttccagc ccctctcaat cctactaacc tttgcctttt ctctgctctt tgatgtcctc
     1561 ttcttacctt tgctgagctt ggtctttttg ctttcccctt tatggaaaat agcagagctc
     1621 aatagtttat ttgtcggttt ggagggcatt attcggtggg tagcaggttt agctagccga
     1681 ccgctcgtct ttggcaagcc cagtctactg gttttggttt tcttattgat tagccttggt
     1741 ctgctctatg atttttggca gcataaaaag gccagagtcg gccttggagt tctgattatc
     1801 ggactttttc tcatcactaa gtgcccgctg accaatgaag tgactgtggt tgacattggc
     1861 caaggcgaca gcattttcct gagagatatg aaaggtcgca atgtgcttat tgatgttggc
     1921 ggtaaggtaa gcttcagtgg caaggaagca tggcagcaag ctcagtctga cagcaatgca
     1981 gagcgtacgc tgattcctta tctaaaaagc cgcggcgtaa gcaaaatcga tacccttgtt
     2041 ctgacccata ccgacacgga ccatatcggt gatttgctgc cggtagccaa aaactttgac
     2101 attggtcaaa tctgggtgtc atcaggtagc ttgactaagg cagactttgt caaaacgtta
     2161 gaaagtcttg gtgttcctgt tcatgtgacc aaggtgggcg atcagattcc tatctgcgat
     2221 agctacctag aggtgctcta ccctagagaa attggtgatg gtggcaataa tgattcgata
     2281 gtcctttatg ggcaactgct ccagaccaga tttctcttta caggtgactt ggagcatgga
     2341 gagctagatt tgattaaggc ctatcctaat ctcagggttg atgtcctcaa agctggacac
     2401 cacggcagta aagggtcttc gtatccagaa tttctggatc atatcaagcc tcagattggc
     2461 cttttctctg ctggaaaaaa taaccgctac aaacatccgc acccagaaac agtcgcgcga
     2521 ttcgatgaga gaaacatcac cacctaccgc acagaccaac aaggcgccat ccgctttcac
     2581 ggttggaaaa cctggcaagt agagacggtg agatgaagaa ggttaggtca gattacttga
     2641 gattaatact tatacgtagt cttcagcggt tacatgcttt tgtgattaca actgtctgtc
     2701 tcagcttata cttaatttat aaaataaaga gaaactctgt caactggttg tagggatttt
     2761 tcgtgttcca gtgtctttgt aatgaattga taaaattcaa ataattgaaa aacaaaagtc
     2821 agaatgttat aattttcaat ataaagataa atattacata gaatagagaa ggaacatatg
     2881 ggtaaattat tggcgagccg aatgcgtagt aggagaaagg aactgaattt gtctcaggtc
     2941 gaattggcag caggcatttg tgaacaagct cagattagta aaattgagcg tgatgctgac
     3001 tatagtccag gagtagattt aatatacgcc cttgcgaaaa agttgaacgt aacaatggat
     3061 tacttttttg atgagaatat tcatgttgaa tctgactatc tgactcagtt tcgtgctgtg
     3121 tctaagaaat tcttagattt acgggactac aagtcactaa aatatattta tgagctagaa
     3181 gccgccaaga ctgtaaaact tccactttcc gatcagttgt accttcaatg gatcgaggct
     3241 gttgtgctat ttaatcacga tcataagcag agtgaagcca tcaaaaaact tgaaagtatt
     3301 ttatcaaaat acagcgaata tgactgggaa tacttgaata tttcaaacag tttattacac
     3361 ttttattttc aaacagatga gcgctcaaaa tttgaagaag tttataatca tatgactaat
     3421 ctttttaaga gtgttaaagt tcggacaatt gatgaacttg aaatattgat taaatgtcgg
     3481 tataactttt gtcgttattt atggctgaat catcaaacgg agacagctat aagcgaaacc
     3541 cttgagacaa ttgatatttg cctcaagaat aatagttttt attgtttagc taatctctac
     3601 tgtttgcttg gaaatgttag tgaagatttt gctcataaag atgtcgttaa acagtacttt
     3661 gtaacggcac aacatgtcta ccaacttgaa cgtaatgata agatggcatt ggagcttgag
     3721 cgttttatca atgaaacatt cccagcatag taattataga caactcagtt cttactgagt
     3781 ttttatatta tggcgtgaca aggctttggg cttccatttc tttttcctga tataatctca
     3841 ttgttcaggt tagataagga ggaaaaagaa tggctaaaac cagttacagt ttatcacata
     3901 ctaaatggat gtgcaaatat catatagttt tcacccctaa gtatcgcaga aaatctatct
     3961 actacaaaat aagacaagac ttgattgaaa ttttccgtca tctatgcaaa tataaaggcg
     4021 ttgaaatcat tgagggacac atgatgccag atcatgttca tatgcttgtt cttattccac
     4081 caaaactttc gatttccgat tttatgggat atttgaaaag caaaagtgcc ttgatgattt
     4141 tcgataaaca tgcaaatcta aagtataagt atggaaatcg aaaattttgg gcaaggggtt
     4201 actacgtcag tacagtcgga ttaaacgaaa aaactgttgc gaaggacatt cgcgagcagg
     4261 agaaagacga cattgcactc gataggttga gtgttaaaga atatgaagat ccgttttcag
     4321 atggcggatt cagaacacga taaaaacccg ttgttacggg cattagtcaa gtaataaaag
     4381 cactaacctg aacgaagttc agcgagcgtc tttaggcgtc gctggaggaa aacggcttac
     4441 agccggtgta caagccaccc gttttcacag gtggttatga ctttttgcaa taaaataata
     4501 aaataataaa atatttataa aaatggttga caaataatat tatgcatgat agaatataat
     4561 caagaaatat gaaaggggtt gcaagaaatg aagaaagcaa ctaaaaagtt tataatagct
     4621 attttattag taggtttatt gattataatc cctaagccat cagggacacc aacgccaact
     4681 cctttaggtt ttgatccagg ggaattatat aatattggtg tttagtataa ttattgtagc
     4741 agatagaaca ttgccatgtt taatgacaat tgaggcttag taaactaatt tgtatgattc
     4801 tagtctgcct gttttcagtc attttataaa cccctttggg gtgtttcaat tgataaaatg
     4861 attgtttgaa aaattttgta agtaaaaaaa gtatatcagt ctgtggaaag gagtgaaaaa
     4921 tcaggaaact atattcagac tgatacacca aatgttgttt taatgtactt taggctataa
     4981 tttaaataag gagtaacctc atgttaaaaa ttagaacagt aatcgcaaca gctcttacta
     5041 ttttaatgtt ttctgtagct cttcctgtct tagctgtttc tcaccgtggc ggtgagtgga
     5101 attacggcgg tcatcatgat ccagataact ggggagcatt ctctaattat tatcatgcca
     5161 ctaattatca ctggtcttat gttggaagca ccggaagagc aaatgaacgc actgtttatg
     5221 caggtgcacg tagtgctgca catgccttca taaacacaaa ttttggagaa catgttaact
     5281 ttgatgcggg gtggtaaaat gtattctaca ttgcgtgtga tgctaacaat tttagtagcg
     5341 cattaacctt ttagggggca ggattactcc tgcccttttt ctagaaagag gactacttat
     5401 gaagagaagt tttgttgttc tatctaattt attggtcact ctctttttcg tatgggtaca
     5461 taccatttgg gaggatacat atgtttcaca ctattatcct aacatctcta tatttagttc
     5521 ttcccccgaa gctacctttg acaaggttgc agatagtcta tcaaaattag cggaggaaac
     5581 agacagtctc attgcccttc agcatcagga tcctgactcc gaggggaaac ctgctttttc
     5641 ttatacggtt tttgggcagg gagaattacc tgaaaatctt tctgaaaaat cactcgaagc
     5701 tgctgccaat ggtagtttag ttactaatta ttttatcttt cgtggtaaat taagcgtaga
     5761 gcgactaaaa gaagagctta gtcgtgtagg gttgactaaa ccatttgtga tgaagccttc
     5821 cagtctcaaa atcctagctc ttgtatttac ccccgggttt caatttcttg ctttgttaat
     5881 ttttctgtta acttttggag cgcttagttt gattagtcaa atcctaaact tacgaaccgc
     5941 aggtatccgt ctaatatctg gtgaaaaccg gtggaaaatt tttttgagac ccctctctca
     6001 agatatcatc catacagcga taggcttgat tgtaggtttt ctctttgcag ttgttttgaa
     6061 acagtttgta tcgtttcctt tggtaacata tcagactatc ggaactggat tggttgttta
     6121 taatctctgt cttctcctca tcactttatt ttttgctgga ctatttgcga ttgggattaa
     6181 aaaagttcct ttaatgcagg tcattaaagg tcaagttcct gttcgaggga tcatcagtct
     6241 gattttaatc ggtcaattgt tagctgtcat tatcgtttcg attggtgtta gccgaacctc
     6301 tatttattcc aaagcttggc aacagcaaga acaggggcga gaagcctggc gccaagaaag
     6361 taacttggtt gctctatcta tggctcgtgg tggggcagac cgtagacaag gtcaagaaga
     6421 acttgctagg gaacagaaag tatggttcaa tctcgttgga caggcagttt cagagcaggg
     6481 cggtttatta tctcaacata atctagctga tagggcttgg caaaatggag taagctcttc
     6541 gaaaaatttt tcaacatcta ctgagtggca agactatagt ccacagggta atgtgttgat
     6601 tgttacacct caatatttga aacagcaaca aattgccgtt gctcctgaga ttgaggaaaa
     6661 agtggctcat ttggctacag gagaatttgt ccttcttcta ccagagcatc ttcgctcaga
     6721 ggaaagtcac tataagaaca tttttgaaga ggatatcagc catcgtatgt ctagtcagga
     6781 tacccgtcag gaaatggttg ctactgtatc ttatctggaa tctggtcatg accgatttgt
     6841 ctataataca acacctatct cttaccagca gcttctaaga gatccgatca ttgttgtgct
     6901 aacacctcaa tcaacggggg agcaatcttt ctccttttgg gagcaatcgg tgagcaactt
     6961 tttctttttt gaaaatttga agaatgctca agcccttatc gagaaatatg gtattgagaa
     7021 ttgggtagca gagctaaaac cggcttacca gatccatcaa acgttattgg ataatctcaa
     7081 aagagaaatg tggacaatgg cggcaggagc tatattaggt acactgactt ctatactgat
     7141 gtttaacacc atgaatcaac tctactttga ggaatttaga cgtgatatct tcattaagcg
     7201 cataagtggt cttcgttttt gggagattca taaaaagtat ctcttagccc aactgttcgt
     7261 tttcttcctt ggttttcttg tcagtatttt cctagctacg gatatattga tagcattctt
     7321 ggtactattg ttatttattg gaaacgcgat tttacagtta cattttcaga tgcacaagga
     7381 aaataagatg tccacgctta ttttgaaagg agcctaagat gattcgatta gaaaaagtga
     7441 gtaaacagtt tggcgagcgt ttcttattct cagatctctc gatgactttt gaggctggaa
     7501 aggtttatgc gctcatcggc tcaagtggaa gtggaaaaac caccctcatg aatatgatgg
     7561 ctaaacttga aacttatgat ggcaatatct ttttccgcaa taaaaactta aatcagttca
     7621 aatccagcga gttcttccgt catgaactag gttacctttt tcaaaatttt ggtttaatag
     7681 aaagtcaaac cataaaagaa aatctaaagc tggggctgat tggtcaaaaa ttgagtaagc
     7741 gtgaacaaca acaaaaagaa gaagaagctt tgaatcgtgt tggattatcc tatttgaatt
     7801 tggacaagag aatcttcgaa ctatctggtg gtgaatccca acgtgttgcc ttggccaagg
     7861 tcatcttaaa gaatcctcct tttattttag cagatgaacc cactgcctcg atcgaccctg
     7921 aaactgccaa ggctattatg gccattctcc tatccttgag gaatgaaaat agacttatca
     7981 ttgtcgcaac ccataatcca actatttggg aaatggctga tgagattatc tctatggata
     8041 gattgtaaac tagaatccaa aaattgtcat aggattcaac taatttttcc tatgagttga
     8101 tgattaaatc aatatgatgg atttatagat gaggagctag tgatggaaaa ttttaaatac
     8161 tacataaaaa aagccagact cgtccaataa ttgggagtta agcaatttcc tagtagttga
     8221 aaatcatgct gatgatgttt tatacttctt tagattttag atagtatatg atatactgat
     8281 tttatgacat acgaattaga ttttagaaaa cgcgtcattg cttacgttca ggaaggacat
     8341 accaaaaagg aaacgagtaa gttatttgga attagtccca ataccctcta tctctgggaa
     8401 aaacaactca gagagttgaa tagcttagaa tgtcaacccc gtaagcgtaa accattcaaa
     8461 ataccgctgg aaaagttgga acaatttgtt caacaatatc ccgacgcttt tttacgtgaa
     8521 atagccgaac attttgattg tagtgttccc tctgtgtgga ctgctttgaa aaaattagac
     8581 atcactttaa aaaaagacga tgagctttaa agaacaagat gtccataagg tccagcaata
     8641 ccttgaggtt ttagcctgct tttcaaatac tccaattgtt tatattgatg agacaggcat
     8701 tgatacctat ctctaccgta aacaaggtag agccaagcgt ggtcaaaaga tttttgataa
     8761 gaagagtgga cgtcgttttg agcgaacttc tttagtggca ggacagattg gaaatcaact
     8821 cattgcgcca atgatgtata aagagagtat gacaagtgac ttctttgtcg cttggtttga
     8881 gcaacagttg atccctagcc taagggaacc tcatcttgtg attatggata atgcttcctt
     8941 tcatcccaaa tccagactgg ataacattgg tattcaacat catcactatt ttttgccact
     9001 accaccttat tcccctgaac taaacccaat tgaacaaact tgggcagttt tgaagtacca
     9061 tataacagag ctgttaagaa aactctctag catttccgaa tgttttgaag actattttaa
     9121 aacttaatga ctatatctct gcatatcatc attaataaat aaaaaatagt agatatagtt
     9181 attttgtttc cctttcaaaa ccaaagcatc tctattccaa cttgctataa tggagagagt
     9241 atagaaattt taagtgatga tgtcaattgg gacaaactgg aattccaaga atttccaaga
     9301 tattgtagct aatgtagcaa aattgtaatg tttgtaatat acaaaaacat caagtaaaat
     9361 tggtttactg taattgttaa ttttagtcaa aaatgaaatg gagactttgt tatgacgaaa
     9421 agcactaaaa agattactgg aattgtaggt aagcgcctat gacatacaaa aacatgttat
     9481 taggtaaaat gaagtactta atcctgtttt tgattgccat tcagagcgca ttatttgctc
     9541 tgatggcaat tttttttaca gctgttcagt atgaggagtc atggaaaaat tataaccgta
     9601 attctggaac agttactgtc tatcttcaaa gattatcgga agaacagtcg caaagtgtat
     9661 tccaatattt tttggagcaa accgacttat ctatttggac gaaacgtact acaaatagta
     9721 gtagggatgg tgcaacaaat agaatgtata tagatattct tgggaatcct gcgggttttt
     9781 cagatcttac aaacggtggt aaaatgattt tatcacgcca acaaatttca gatttattat
     9841 cccatagtga caataacttg actattggac ttgataaggg gtctgataat atgctctacg
     9901 aattaccaag tttgttattt acaacgcctg ttgtcataaa tcggttagac tatatttttc
     9961 aagaaacaaa tactataaac gggatatatc atatcaatgg tcttcaggac gatctctcaa
    10021 gagaaacatt tttgtcaaac ctctcagcca ttactgggat ttctgttgaa gacttgaccc
    10081 gagagagttt tggcagtaac actgttgagg gaattgtgcc aatcgtttta gctgctagta
    10141 ttgcagtaaa tgctatggtc cttcttgttc ttttcctaat atgtgtttta caatccttta
    10201 aacattttgg gacattgata ctattgggat gggataggaa agagttgtgg tttgccctct
    10261 tcaaagattc tcttctgttt tctatttata tcgcacc
//
