LOCUS       NZ_LT906439            20876 bp    DNA     circular CON 15-DEC-2024
DEFINITION  Streptococcus merionis strain NCTC13788 chromosome 1, complete
            sequence.
ACCESSION   NZ_LT906439
VERSION     NZ_LT906439.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus merionis
  ORGANISM  Streptococcus merionis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2017) WTSI, Pathogen Informatics, Wellcome Trust
            Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900187085.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 13:40:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,228
            CDSs (total)                      :: 2,160
            Genes (coding)                    :: 2,111
            CDSs (with protein)               :: 2,111
            Genes (RNA)                       :: 68
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 27 of 49
            Pseudo Genes (incomplete)         :: 28 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 16 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:09:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 57537
            Orig. end                         :: 78413
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT906439.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20876
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="1"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20876
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20876
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10876](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10876
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    complement(456..467)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.28"
                     /tool="antismash"
     misc_feature    599..615
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.52"
                     /tool="antismash"
     misc_feature    complement(666..681)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.36"
                     /tool="antismash"
     gene            complement(798..3038)
                     /locus_tag="CKV83_RS00285"
                     /old_locus_tag="SAMEA4412692_00059"
     CDS             complement(798..3038)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1223: ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605555.1"
                     /locus_tag="CKV83_RS00285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00059"
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="WP_026216877.1"
                     /transl_table=11
                     /translation="MLCHNCKMNDATIHLYSNYNGRQQQVDLCQNCYQIMKTDPENPIL
                     RGLSQIQNQQRSPFDDFFDNLSNFQHPSQAPGNQIPPTQAGRNNSNRGGNQQPPKKGLL
                     DEFGINVTEIARRGGIDPVIGRDEEIVRVIEILNRRTKNNPVLIGEPGVGKTAVVEGLA
                     QKIVDADVPHKLQNKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEIRERQDVILFIDEI
                     HEIVGAGSAGDGNMDAGNILKPALARGELQLVGATTLNEYRIIEKDAALERRMQPVRVD
                     EPSVEETITILKGIQKRYEDYHHVRYTDEAIEAAANLSNRYIQDRFLPDKAIDLLDESG
                     SKMNLTLNFVDPKEIDQRLVEAENLKAQATRDEDYEKAAYFRDQIAKYKEMQKATLDEK
                     DIPVITEKEIEAIVERKTNIPVGDLKEKEQSQLVNLASDLKAHVIGQDEAVDKIAKAIR
                     RNRVGLGAPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSADSMIRFDMSEYMEKHSTA
                     KLVGAPPGYVGYDEAGQLTEKVRRNPYSLILLDEIEKAHPDVMHMFLQVLDDGRLTDGQ
                     GRTVSFKDTIIIMTSNAGTGKVEASVGFGAARENRTNSVLGQLGDFFSPEFMNRFDGII
                     EFQALSKDNLLQIVDLMLNNVNDRLANNGISLHVTDKVKEKLVDLGYDPKMGARPLRRT
                     IQDHIEDAITDFYLENPTEKDLRAVMTSSGNIAIKPAKKTEKTNTTDEETDQ"
     misc_feature    complement(3431..3447)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.12"
                     /tool="antismash"
     gene            3504..3734
                     /locus_tag="CKV83_RS00290"
                     /old_locus_tag="SAMEA4412692_00060"
     CDS             3504..3734
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /locus_tag="CKV83_RS00290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00060"
                     /product="DUF1797 family protein"
                     /protein_id="WP_018372955.1"
                     /transl_table=11
                     /translation="MESHLVRIINRLELMAKDGGTLKRNFEREGVVVAEVSYSYDEENG
                     SLFTLRDIEAHETYTFDSIDLIAMEIYELLY"
     gene            complement(3807..4520)
                     /locus_tag="CKV83_RS00295"
                     /old_locus_tag="SAMEA4412692_00061"
     CDS             complement(3807..4520)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372956.1"
                     /locus_tag="CKV83_RS00295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00061"
                     /product="B3/4 domain-containing protein"
                     /protein_id="WP_018372956.1"
                     /transl_table=11
                     /translation="MLSAIQIDETFWSRFPEGRIVVMTLKGIDNRVIPEKEAYFKDLLQ
                     KGKESSLQFLKDDNFSQNPVVAEWREAYSQFKTKKGARSSIEAMLKRAAQDRDFNPINP
                     LVDLYNYVSLTYATPVGAEDMDKVEGTLRLAETAGGDSFKPLGAEEDAPTLAGEVAWLD
                     DAGAVCRCLNWREAQRTMLTEDTQNAIVIMETVNENQAARAQTAMNTLKDLCQDYFGVT
                     GEILVLSSSLASATI"
     misc_feature    4559..4572
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.33"
                     /tool="antismash"
     gene            4992..5852
                     /locus_tag="CKV83_RS00300"
                     /old_locus_tag="SAMEA4412692_00062"
     CDS             4992..5852
                     /GO_function="GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909467.1"
                     /locus_tag="CKV83_RS00300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00062"
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="WP_026216878.1"
                     /transl_table=11
                     /translation="MVLIDGKALAVKIQGKLAEKVARLKEEKGVVPGLVVILVGEDPAS
                     QVYVRNKERSALAAGFRSEVVRVPATISQEELLALVERYIQDASWHGILVQLPLPQHID
                     EEAILHAIDPKKDVDGFHPTNMGKLWSGHAVMIPSTPAGIMEMFKEYEIALAGKRAVVI
                     GRSNIVGKPMAQMLLDANATVTIAHSQTQNLAELAKTADILVVAIGRGHFVTKDFVKPG
                     AVVIDVGMNRDANGKLIGDVAFDEVKDVASYITPVPGGVGPMTITMLLEQTYQACLLAG
                     EAGSQ"
     gene            5892..6278
                     /locus_tag="CKV83_RS00305"
                     /old_locus_tag="SAMEA4412692_00063"
     CDS             5892..6278
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012792.5"
                     /locus_tag="CKV83_RS00305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00063"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_018372958.1"
                     /transl_table=11
                     /translation="MLYREIQDSDIERLEELYQKEKWTSFKSHMIQSLKDKSHWVIAEE
                     DGNICGFARYITDGVLTVYLCELIVATDFRQQGIGRSLIQEIFSRHVGLRIDLASFNDD
                     FYDRLSFRKIGSAYRNYGERNTDS"
     gene            6253..7107
                     /locus_tag="CKV83_RS00310"
                     /old_locus_tag="SAMEA4412692_00064"
     CDS             6253..7107
                     /GO_function="GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372959.1"
                     /locus_tag="CKV83_RS00310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00064"
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="WP_018372959.1"
                     /transl_table=11
                     /translation="MVKEIQTVDVNLIRKVILPRSLHSHKGNYGRVLLIGGCYPYGGAI
                     LMAARSCVYSGAGLVTVATERDNITALHAQLPEAMAFDIEECPLLEAQLAGAEVIVIGP
                     GLGESDLAREVFTSVLSAVRSSQVLIIDGSALTLLAEKLDLVNSFAGQMILTPHQKEWE
                     RLSGLAVEDQLVEANLQALKQFPQGSILIAKRYQTQILQLGQTPRQITIGGPYQATGGM
                     GDTLAGMIAGFVGQFKNDRLDAVSAAVYAHSAIARELSQTNYVTLPSQIAEQIPVFMRS
                     ILP"
     tRNA            7162..7249
                     /anticodon=(pos:64733..64735,aa:Ser,seq:gga)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="CKV83_RS00315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SAMEA4412692_00065"
                     /product="tRNA-Ser"
     gene            7162..7249
                     /locus_tag="CKV83_RS00315"
                     /old_locus_tag="SAMEA4412692_00065"
     gene            8472..9815
                     /gene="xseA"
                     /locus_tag="CKV83_RS00320"
                     /old_locus_tag="SAMEA4412692_00066"
     CDS             8472..9815
                     /EC_number="3.1.11.6"
                     /GO_component="GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA]"
                     /GO_function="GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /GO_process="GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="xseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730498.1"
                     /locus_tag="CKV83_RS00320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00066"
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="WP_018372960.1"
                     /transl_table=11
                     /translation="MTEYLTVSHLTKYLKLKFDKDPYLARVYLTGQVSNFRKRPNHQYF
                     SLKDEGAVIQATIWGGIYQKLGFELEEGMKINVVGRVQLYEPSGSYSIIIERAEPDGLG
                     ALAVQFEQLKKRLGDEGLFKAEWKQALPQFVRKIGVITSPSGAVIRDIITTVSRRFPGV
                     EILLYPTVVQGAGAAPAIVANIQKANNRGDLDLLIVGRGGGSIEDLWAFNEESVVRAIF
                     ESRLPIISSVGHETDTTLADFVADRRAATPTAAAELATPVTKADLLNHLRQQQNRLYTA
                     SLNRLRFYQERLRKLSQSVIFRQPERLYDGYLQKLDHLTDKLQQNMQKAYQLNQQALRL
                     LQERLKAQRLDQEIKRQQVNLQQQTRLLKSNMAILYDNKMNKVKQLSEAMLMLDTNRIV
                     ARGYAMVYQKGRVVESVKGVAIGSQATIQMRDGQIEVEVKDVKDEKNI"
     gene            9790..10008
                     /locus_tag="CKV83_RS00325"
                     /old_locus_tag="SAMEA4412692_00067"
     CDS             9790..10008
                     /EC_number="3.1.11.6"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372961.1"
                     /locus_tag="CKV83_RS00325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00067"
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="WP_018372961.1"
                     /transl_table=11
                     /translation="MSKTKKTFEDNLAELETIVGRLESGDIALEEALSEFQKGMVLSKE
                     LQATLAKAEETLVKVMQEDGTEVEFNG"
     gene            10001..10876
                     /locus_tag="CKV83_RS00330"
                     /old_locus_tag="SAMEA4412692_00068"
     CDS             10001..10876
                     /EC_number="2.5.1.-"
                     /GO_function="GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907032.1"
                     /locus_tag="CKV83_RS00330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00068"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_018372962.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1e-52, bitscore:
                     176.0, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDRQGKVSGVEQAIRSYYEEAQVAERLVEAVLYSVNAGGKRIRPL
                     FFLEMVEALGQTLTQAHFQVAASVEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFDEA
                     TAILAGDSLFLDPFGLIARADFPAEIRINLVQELSDSSGTYGMVAGQTLDMMSEGKLID
                     LESLQTIHANKTGKLLTFSFVAAGLVAEVSTDVLTKLRQVGELVGLAFQVRDDILDLTA
                     DFEDLGKTPQKDLLAEKSTYPALLGLVESQQFLNHTLDQVQNLLKEIAEKCPFQAKQVG
                     QLIESLRLNG"
     gene            10869..11687
                     /locus_tag="CKV83_RS00335"
                     /old_locus_tag="SAMEA4412692_00069"
     CDS             10869..11687
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000167.1"
                     /locus_tag="CKV83_RS00335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00069"
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="WP_018372963.1"
                     /transl_table=11
                     /translation="MAKERVDVLAHRQGLFETREQAKRGVMAGLVIASVNGHRYDKPGE
                     KIDEDTELKLKGEKLKYVSRGGLKLEKALAQFGISVAEQLTLDIGASTGGFTDVMLQAG
                     AKMVYAVDVGTNQLAWKLRQDPRVISMEQYNFRHAKLDDFPYGQPMFASIDVSFISLSL
                     ILPALTQILADDGQVVALIKPQFEAGREQIGKNGIVKDKKVHLQVLENVSTFALESGFS
                     VQNLDFSPIQGGHGNIEYLAHLQKSKAQEMAYSQSLAELVEQSHQEFKHE"
     gene            11680..12108
                     /locus_tag="CKV83_RS00340"
                     /old_locus_tag="SAMEA4412692_00070"
     CDS             11680..12108
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372964.1"
                     /locus_tag="CKV83_RS00340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00070"
                     /product="arginine repressor"
                     /protein_id="WP_018372964.1"
                     /transl_table=11
                     /translation="MNKKERLQIISDLISENAIDTQEEIVTRLQAIGIKATQATVSRDI
                     KTLGIVKVPSESGYIYDLPHSRKRKIRPQKKYLRDFAVQAPFIHLKVEPGTSAVVKRQL
                     SEQLPDQLFSVIADDDSILMVVKEGAAIEDVLAKVKEI"
     gene            12109..13767
                     /gene="recN"
                     /locus_tag="CKV83_RS00345"
                     /old_locus_tag="SAMEA4412692_00071"
     CDS             12109..13767
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896130.1"
                     /locus_tag="CKV83_RS00345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00071"
                     /product="DNA repair protein RecN"
                     /protein_id="WP_018372965.1"
                     /transl_table=11
                     /translation="MLLAVSIKNFAIIEQISLDFQTGMTVLTGETGAGKSIIIDAMNMM
                     LGARATTDAIRHGATKAEIEGLFSLEKSSAIDKILEEQGIEVSNELIIRREIFQNGRSV
                     SRINGQMVNLTVLRAVGQHLVDIHGQHDQEELMKTQSHISMLDQFGDEGFQAQKAQYEE
                     TFEHYRVLRRQVLTQQKNEQENKARIEMLRFQLDEIESAELEAGEDERLHKERDRLVNH
                     KNIADTLANAFTMLDDDDLGTLRNLRSAMNDLQSIESYEPDYKNMADQVVESYYVLDDV
                     GKRLGSILDNLDFDGNRLLQVESRLDLLNSITRKYGGSVDDVLTYFQKISEEYQLLTGI
                     LPGQGDLEQELKRLEKDLITQASRLSQARHDLAKILEAEIKQELRDLYMEKAEFQVTFT
                     KAKFSKVGNEQVEFYISTNPGEGFKPLVKVASGGELSRLMLAIKSAFSRKENKTSIVFD
                     EVDTGVSGRVAQAIAQKIYKIAQAGQVLAISHLPQVIAIADYQFFIEKISDETSTVSRV
                     RLLTLEERVEEIAKMIAGDSLTPAALEQARVLLEK"
     gene            complement(14333..15169)
                     /locus_tag="CKV83_RS00350"
                     /old_locus_tag="SAMEA4412692_00072"
     CDS             complement(14333..15169)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1179: hydrolase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851775.1"
                     /locus_tag="CKV83_RS00350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00072"
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="WP_018372966.1"
                     /transl_table=11
                     /translation="MSKILFLDVDGTIVNYENQIPASTIKAIKLARKNGHKVYACTGRS
                     RAEMQPELWDMGIDGMIGGNGNYIESDGEVVYHNSLTLEDCTAIVDYLHEHGLEFYLES
                     NSGLYASENFEERSLPVMREYARRKGYENAETLMTKDALHGIVFGETNLYRDDVNKISF
                     ILESYQDYLDAKAHFTNFEVNTWGGKGEIALFGDVGVKDIDKAFAVEQLLSHLQAAQVD
                     TFAFGDAKIDIPMFNYCAYSVAMGSGGPEAKEAADYITTGVDEDGIYNAFKHLGLI"
     misc_feature    15266..15280
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.06"
                     /tool="antismash"
     gene            15365..15859
                     /gene="ybeY"
                     /locus_tag="CKV83_RS00355"
                     /old_locus_tag="SAMEA4412692_00073"
     CDS             15365..15859
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ybeY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087312.1"
                     /locus_tag="CKV83_RS00355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00073"
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="WP_018372967.1"
                     /transl_table=11
                     /translation="MYVEMHDETGQVTKAIQDQTLALLDFAAKRLDKQQKEMAVTFVDN
                     ARIQEINKLYRDKDQPTDVVSLEYKPDSEIFFEEEDLEDNPELAEMIAEFDSYLGELYI
                     SVDKAREQAESYGHTFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILSAYGLS
                     R"
     gene            15843..16247
                     /locus_tag="CKV83_RS00360"
                     /old_locus_tag="SAMEA4412692_00074"
     CDS             15843..16247
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009568697.1"
                     /locus_tag="CKV83_RS00360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00074"
                     /product="diacylglycerol kinase family protein"
                     /protein_id="WP_018372968.1"
                     /transl_table=11
                     /translation="MDYHDKNNSRKKWKNRDLITSMEFAITGLLTAFKEERNMRKHGVS
                     AVLVVLLGLLFQVSAIEWLFLLLAVSLVIAFEIVNSAIENVVDLASDYHFSMLAKHAKD
                     MAAGAVLFVSGYALLTGAIIFLPKVWNLLF"
     gene            16599..17495
                     /gene="era"
                     /locus_tag="CKV83_RS00365"
                     /old_locus_tag="SAMEA4412692_00075"
     CDS             16599..17495
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="era"
                     /gene_functions="other (smcogs) SMCOG1227: ribosome
                     biogenesis GTP-binding protein YsxC"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516037.1"
                     /locus_tag="CKV83_RS00365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00075"
                     /product="GTPase Era"
                     /protein_id="WP_026216879.1"
                     /transl_table=11
                     /translation="MFKSGFVAILGRPNVGKSTFLNYVMGQKIAIMSDKAQTTRNKIMG
                     IYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEARGKGDDMI
                     IERLKNAKVPVILVINKIDKVHPDQLLEQIEDFKDQMDFKEIVPISATQGNNVNRLMAI
                     LKENLPEGYQYFPEDQITDHPERFLVSEMIREKVLHLTREEIPHSVAVVVDSMKRDEAT
                     DKVHIRATIMVERDSQKGIIIGKQGSMLKKIGSMARRDIELMLGDKVFLETWVKVKKNW
                     RDKKLDLADFGYNQKEY"
     gene            17789..18406
                     /locus_tag="CKV83_RS00370"
                     /old_locus_tag="SAMEA4412692_00076"
     CDS             17789..18406
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1101: phosphoglycerate mutase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372970.1"
                     /locus_tag="CKV83_RS00370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00076"
                     /product="histidine phosphatase family protein"
                     /protein_id="WP_018372970.1"
                     /transl_table=11
                     /translation="MRRIITVQHTESVHHTNGMIGSWTDWELTATGKEQAERIAKRLKA
                     DYPDVNFTLYSSDLKRAKQTAQAIGDVFGIPIHLSGALRERNLGRACGQSVQWLKDNIE
                     SHETDIDSRLFSDAETRREAWNRLKPFYDELLNQPHENIILVSHGDLLSLFNAMWLGLE
                     PEFLNRADLYGNSGGVSLLLEKQGKRVIKSMSDMSYIRPNQE"
     gene            complement(18845..19837)
                     /gene="asnA"
                     /locus_tag="CKV83_RS00375"
                     /old_locus_tag="SAMEA4412692_00077"
     CDS             complement(18845..19837)
                     /EC_number="6.3.1.1"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="asnA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /locus_tag="CKV83_RS00375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00077"
                     /product="aspartate--ammonia ligase"
                     /protein_id="WP_018372971.1"
                     /transl_table=11
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEIVEVQGPILSRVGDGMQ
                     DNLSGVENPVSVHVKLIPEHTFEVVHSLAKWKRHTLARFGFNEGEGLFVHMKALRPDED
                     DLDPTHSIYVDQWDWEKVIPDGKRNLAYLKQTVELIYKAIRLTELAVEARYDIESVLPK
                     QITFIHSEELVERYPDLTPKEREKAITKEFGAVFLIGIGGELADGQPHDGRAPDYDDWT
                     TPTENGYKGLNGDILVWNEALGSAFELSSMGIRVDEDALKRQCAIKGDEDRLELEWHRA
                     LLNGLFPLSIGGGIGQSRLAMFLLRKKHIGEVQSSVWPKAVRAEFDNIL"
     gene            20059..20535
                     /locus_tag="CKV83_RS00380"
                     /old_locus_tag="SAMEA4412692_00078"
     CDS             20059..20535
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009568694.1"
                     /locus_tag="CKV83_RS00380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA4412692_00078"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_018372972.1"
                     /transl_table=11
                     /translation="MTQDYISYIRSKVGHDKIILNFAGGILADGNGRILLQKRSDFGSY
                     GLPGGAMELGESSKEACEREFFEETGIKVEATRLLNVYTKDENHYPNGDEVQTVVILYE
                     VKALEAFDITDFHNGETLEVGFYSEAEIAELEPVFDKHRRMIAEYFADTFALGN"
ORIGIN
        1 tttactggga ataatggcat aaacaccata acgatttttg taagttgcac catcaacttt
       61 gtggccaaaa gtagggttag acataagtag gctccttgac tcgactaagg ataaacaaga
      121 actagttact agtaaattcc tttgacttag gaagtcgttt tttgataatc aaagcatttt
      181 ttgaaagaat gcgaactgtg tttcaaaaaa aacgaacgct gagtggtgtg aaactagcct
      241 tggatgatag tgttgggctg tgtataactt ctcacttgga tttatccgga ttcgagttct
      301 tgcttgtcac tttctttttc tattatacta aaaaataatg attttggagt gatttttgtc
      361 aaaaaggctg tgaaatcaac tctaaccagc taagcaagcg ctctttgctg cttttggtca
      421 ggcaaggaga aggtgagctc aaatttagca agcctgcgtt caattattta ggagatacaa
      481 gtgtaagctt gaggcgtttt ttgtctcaag cttgcgttgt tagaagtatc ctatacagtt
      541 gagtagagct gtaaagaccg agatagtcag cacattcgag ttcgcttggc aggttcattg
      601 gtgataaaat atgcaaggct aaggctaagt aagttggcct agtcagcaaa acttattccc
      661 accccttttg tctagtcctt tagaccacat aattttgtaa tggccaaagc tttgggagta
      721 agggctaaac catcatgatt gtctgtttta gcttccaaac cattgactga gaaaaatagt
      781 ttaggtcgta agcaagtcta ttggtcggtc tcctcatcag ttgtgttggt tttttcagtt
      841 tttttagcag gctttatcgc aatgttgcct gatgaggtca taactgctcg gaggtctttt
      901 tcggttggat tttctagata gaagtccgtg atggcgtctt caatatgatc ttggatagtt
      961 cggcggagag gacgagctcc cattttcgga tcatagccca gatcaaccag cttttccttg
     1021 accttatcgg tcacgtgtaa gctgatgccg ttgttagcaa gacgatcgtt aacgttgtta
     1081 agcatgagat caacgatttg gagaagattg tctttgctga gagcttgaaa ttcgatgata
     1141 ccgtcaaatc ggttcatgaa ttctgggctg aagaagtcgc ccagctgacc aagaacagag
     1201 ttcgtccgat tttctctggc tgcaccaaag ccaacagagg cttcgacctt accagtaccg
     1261 gcatttgacg tcatgatgat aatggtgtct ttgaagctga ctgtgcggcc ttggccatcg
     1321 gttaagcggc catcatctag aacctgaagg aacatgtgca tgacatcagg gtgcgctttt
     1381 tcaatttcat caagaagaat gagtgagtaa ggattgcggc ggactttctc ggtcagttgt
     1441 ccagcttcgt catagccaac ataacctgga ggagcaccga ccaatttggc tgtgctgtgt
     1501 ttttccatat attcagacat gtcaaaacga atcatgctat ccgctgagcc gaagagttca
     1561 atggctaact gtttcgataa ctctgtttta ccgacacctg ttgggccgac aaaaaggaag
     1621 gagccgattg gacggtttgg agcaccaagt ccgacacggt tgcgccgaat ggctttggca
     1681 atcttatcaa ctgcttcatc ttgaccaatg acatgggctt taaggtcgct cgcaaggttg
     1741 acaagttgac tctgctcttt ttctttgaga tctccgacag ggatattggt tttgcgctca
     1801 actattgctt caatttcttt ttcggtaatg acaggaatgt ctttctcatc aagggtggcc
     1861 ttctgcattt ctttgtattt ggcaatctga tcgcggaaat aagcagcttt ttcgtaatct
     1921 tcatcacgag tagcttgagc tttgaggttt tcagcctcaa ccaaacgttg atcaatttct
     1981 ttaggatcca caaaattgag ggtcaaattc atcttggagc ctgactcatc cagaaggtca
     2041 atagccttgt ctggcaagaa gcggtcttga atataacggt tagacagatt agcggcagct
     2101 tcgatggctt catcggtgta gcgcacatgg tggtaatctt cgtagcgttt ttggatgcct
     2161 tttaagatcg taatggtctc ttctacactt ggttcatcga cacggactgg ctgcatgcga
     2221 cgctccagag ccgcatcctt ttcgataatg cggtattcgt tgagagtggt cgcgccgacc
     2281 agttggagct ctccacgagc tagagctggt tttaggatgt tgcctgcatc catattgcca
     2341 tcgccagcgc tacctgctcc gacaatctcg tgaatttcat cgatgaagag aatgacatct
     2401 tgacgctcac ggatttcttc catcaacttt tgcatgcgct cttcaaattg accacggata
     2461 ccggtgcctt ggacgagact gacgacatca agacggatga cctgcttgtt ttgcaactta
     2521 tgtggtacat cagcatcaac gatcttttga gctaagcctt caacgacagc tgtcttacca
     2581 acaccaggct ctccgattag aacagggttg ttcttggtac gacgattgag gatttcaatg
     2641 acgcgaacaa tctcctcatc acgaccgatg acagggtcaa tgccgccacg gcgagcgatt
     2701 tcggtcacat taataccaaa ttcgtccaaa agtccctttt taggcggttg ttgattgcca
     2761 ccacggttac tgttgtttcg gccagcttga gttggtggaa tttgatttcc aggagcttga
     2821 cttggatgtt ggaaattgct caaattatca aagaagtcat caaacgggct tctttgttgg
     2881 ttttgaattt gagataatcc tcttaaaata ggattttctg ggtcagtttt catgatttgg
     2941 tagcagtttt gacagaggtc aacctgttgt tggcgaccat tgtaattgct atacaggtga
     3001 atggttgcgt cgttcatttt acaattatga caaagcatgg caatacctct ttttctagaa
     3061 tgatttgaac aatctgatta aaggtcaata aaggtcaaat cttaatttta ttatatcact
     3121 tattaaagag aaatcaagtg aaaagattaa tcatatgaat gctattgcct ctatcgtcca
     3181 ttttttcgag gaatttataa gtgcgttaaa tgaatcattg tggctttaga acattgcttt
     3241 cagaaaatca gatgacaaac agtcaacttt tgctccaaat taaataacta ctggaatggt
     3301 tgttgattgc ttgagaagtc agtgtaacga gggatttggc gttatttgag ggctcaaatt
     3361 ttgagaaata agcatttttc ttaggctaag cggtttattt tatggtagaa tagggatata
     3421 gaattagaaa cgcagctgaa attggcgttg tttttgacac agttcaggct aatcaaggca
     3481 gtttcagtta aaaggagaag aacatggaat cacatttggt gagaattatc aatcgtctag
     3541 agttgatggc taaagatggg ggaactctta aacgtaactt tgagcgtgaa ggtgtcgtag
     3601 ttgctgaagt ttcatacagt tatgatgagg aaaatggttc gctctttact cttcgtgaca
     3661 ttgaagcgca tgaaacctat acgtttgact ctattgattt gatcgcgatg gaaatttatg
     3721 agttgcttta ttaatatagt tttaaaataa aagataccaa gatactcggt tgtagccaag
     3781 tgctttggta tctttttttg caaggtttaa atagtagctg acgctaggct gctggatagc
     3841 actaggattt cccctgttac accgaaataa tcttggcaga ggtcttttag agtgttcatg
     3901 gctgtctgag ctcgtgcagc ttggttttca ttgacagttt ccatgataac gatagcattt
     3961 tgagtgtctt ctgtgagcat ggttcgttgg gcttcacgcc aattgagaca gcgacagaca
     4021 gcaccagcat catctaacca agcaacttca ccagcaaggg ttggagcatc ttcttccgca
     4081 ccgagtggct taaaagagtc gccgccagct gtttcagcaa gtctgagagt gccctcaacc
     4141 ttatccatgt cctctgctcc aacaggtgtc gcataggtta gcgaaacata attatagaga
     4201 tcgaccaatg gattgatagg gttaaaatca cggtcttgag ctgcacgctt gagcatggct
     4261 tcaatagatg aacgagcgcc ttttttagtt ttgaattggc tgtaagcttc acgccattca
     4321 gctacaacag gattttgact aaagttatca tcttttaaaa attgaagcga agattctttg
     4381 cctttttgga gtaaatcttt aaaataggcc tctttctcag gaataacacg gttatcaatc
     4441 cctttcaatg tcatgacgac gatacggcct tctgggaatc gagaccaaaa agtctcgtca
     4501 atttgaatag ctgataacat aggaattacc tcaccttctt tttttcttta gtatactctt
     4561 tttaaaataa aagtgcaaga agtggtggat gaaacgttta aatactcttt ggttagcagt
     4621 aaacaacgaa atactttgga ttaatataca aaaacgcaca ttgtcaatta tagttttaaa
     4681 tgggaactct gcctatttta ctttggctag atttctgatc ctcttttgat aagggacttc
     4741 taattgcttt attattcaga tgtcatttgg gatgtcgttt gagggctggt tggtcaggtg
     4801 aagggagcta ttcgtttggt atatatctgt ggccattatc aaggagaact cttatatcaa
     4861 agagagaacg aaggatttta tatccaaagt cttataaggt tcgtaaaaac agtgataagt
     4921 agctcgtttt tttgaaaata gaggctgttt ttgttataat caaaaaaatg aaaagaattg
     4981 agaagcaatc tatggttttg attgatggaa aagctctagc agtgaaaata cagggaaaat
     5041 tggctgaaaa agtagcccgt ttaaaagaag aaaagggtgt ggtgcctggg ctagtcgtga
     5101 ttttagtcgg tgaggatcca gctagtcaag tctatgtcag aaataaggag cgctctgctt
     5161 tagcggccgg ctttaggagt gaggtagtgc gcgtgccagc tacgattagt caggaggagt
     5221 tgctggccct agttgagcgc tatattcagg atgcgtcatg gcacgggatt ttagtgcaac
     5281 tgcccttgcc acagcatatt gatgaagaag ctattttgca cgctattgat cccaaaaaag
     5341 atgttgatgg gtttcatccg accaatatgg gtaagctctg gagtgggcat gcggtcatga
     5401 ttccatctac gccagcaggt attatggaga tgtttaagga atatgagatt gccttagctg
     5461 gaaaacgtgc ggttgttatt ggacgctcca atatcgttgg caaaccgatg gctcaaatgt
     5521 tactagatgc gaatgcgacg gtgacgattg cccattcgca aacacagaat ttggcagaat
     5581 tagctaagac tgctgatatc ttggttgtgg ccattggacg cgggcatttc gtgacaaagg
     5641 attttgttaa gccaggtgcg gttgtcattg atgtgggtat gaaccgtgat gcaaatggca
     5701 agttaatcgg agatgtggct tttgatgagg tcaaggatgt ggcgagctat atcacaccag
     5761 tgccaggagg ggttggtccg atgaccatta ccatgctttt ggagcagacc taccaagcct
     5821 gtcttttagc aggtgaggct ggtagtcagt agcccttgtt aatcaactaa ccgtctaaag
     5881 gagtctaaac gatgctatat agagaaattc aagattcaga tattgaaaga ttagaagaac
     5941 tttatcaaaa ggaaaaatgg acgagtttta agagccacat gattcagtct ttgaaagata
     6001 agtcacactg ggtgattgct gaggaagatg ggaatatttg cggttttgca aggtatataa
     6061 cagatggcgt attgactgtt tatctgtgtg aattgatcgt ggcgactgat tttcgacaac
     6121 aagggattgg ccgctcgctt attcaggaaa tcttcagcag acatgttggt cttagaatag
     6181 acttggcgtc atttaatgat gatttttatg acaggctaag ttttagaaaa atagggagcg
     6241 cctatcgaaa ttatggtgaa agaaatacag acagttgatg tgaatctcat tcgcaaggtc
     6301 attcttccta ggtctcttca tagccacaag gggaattatg gccgtgtact tctcattggg
     6361 ggttgctatc cctatggtgg tgcgattctc atggctgcaa ggtcctgtgt ctacagcgga
     6421 gcaggacttg tgacggtcgc gacagaacgg gataatataa cagctctgca tgctcagttg
     6481 ccagaggcta tggcttttga tatagaggag tgccctcttc ttgaagcgca gttggctggt
     6541 gctgaagtta ttgtgatcgg cccaggactg ggtgaatctg acttggctag ggaagttttt
     6601 acatcagttc tcagcgctgt taggtcatct caggtcctta tcattgatgg ctctgcacta
     6661 acgcttttag ctgagaaatt ggacttggtt aacagctttg ccgggcagat gatcctcacg
     6721 ccacaccaga aagaatggga acgactgagt ggcctagcag tagaagacca gttagtagaa
     6781 gcgaatttac aggctttgaa gcagtttcca caagggagta ttctcattgc caagcgctat
     6841 cagacccaaa tcttgcaatt aggacaaacg cctaggcaaa tcacgatcgg tggtccctat
     6901 caggcgacag gtggcatggg agacacttta gcagggatga ttgcgggttt tgtggggcaa
     6961 tttaagaatg atagattaga tgcggtgtcc gcagcggttt atgcccattc ggctattgct
     7021 cgagagctta gtcagaccaa ctatgtgacg ctacctagtc agattgcgga gcaaattcct
     7081 gtctttatgc gcagcatttt gccttaaagt gagaaaaaac ttgattaatt cagctggtca
     7141 tggtatactg ttacctagtt tggagaatta ctcaagtggc ttaagaggcc accttggaaa
     7201 ggtggtaggt cgtgtaagcg gtgcgtgggt tcgaatccca tgttctcctt aagattaaaa
     7261 ggatcaggct aaaagcctga ttacgtgatt tcccctgata aactagctgg ctactattgt
     7321 taggtctaag agctgtactc ccagctcagt accttggatt taaggctggc ttttttgcta
     7381 ctcatcggtc gtatttcagc cccaatctct ttaaaaggca taaagtatct tacttgtgaa
     7441 tgcaggtttt aaactagtaa aatttaaggt tgagacactt tttgtcccaa cttttttgtg
     7501 tttttaggca ggctaaatgt agtaagagaa ccactgttag attttttgaa agcattctga
     7561 agtagcaaaa atcagttata agtcaaagaa aaataacttt tttgataatt tgtgctgatg
     7621 aaaatgtctt ttgcctcctt atattaaagg gccttgttca attctttatc ttaattttta
     7681 aggaaaaggt taggtatttg gagaggcttt ttggtataat aatgattagt gaaacaaaat
     7741 tctgtaacat gggctaaaaa gctagatcga gtttagaatg gctaggacgg tagccttctg
     7801 gatttttttc gaattcatct gtgaaaatcc tagctgccct ggctggggtg cgtttatggg
     7861 acttgggtaa ggtataagtc tcgtttcgtt tatagtttga acagattgac gcagtagttg
     7921 tctggtttgt agagcgagtc cggaacaaaa ggcactccca tcttctgtat tttttgttta
     7981 gtggcaagac gcagtggttg agtgggctct aatacattga taaatcaaat tttagagccc
     8041 tactcaacgg ttcgctgtct cactccctga cttaccgcca tttttcaacg cttttcttga
     8101 cgggctttgt atcttacaac tgaattcggc ctaaaatcct cgataaaatt cgaaaacctc
     8161 ctcaagtcca aagacttgtc gtctgtcttc tcatttttca tcagattttt tcacggcctt
     8221 atatcttaat caactgaaca cgggctaaaa gctcggaaaa atgataaatc ttcctagagt
     8281 gaagacactc attgtcagat ttcctatttt tcagtcgctt tttatacgcc ctttgtatct
     8341 taaataactg gacacgccct aaaaagcttg ataaaattca aaaatttcct agagtgtcta
     8401 cactcttcgt caattttttc attttccgtc gcttttctaa acgggctttg tatcttgaat
     8461 aaggagggac gatgactgag tatctgacgg tgtcgcacct aactaagtat ttgaaattaa
     8521 agtttgacaa ggatccttat ctggctcgcg tctatttgac tggtcaagtc tccaactttc
     8581 gtaagcgccc caaccaccag tacttttcgc ttaaggacga aggggctgtg atccaggcta
     8641 ctatttgggg tggaatttat caaaaattgg gctttgaact ggaagaaggg atgaaaatca
     8701 atgtggttgg tagagtacag ctctatgaac cgagcggctc ctactccatt atcatcgaaa
     8761 gggctgagcc agatggctta ggtgctttag cggttcaatt tgaacagcta aagaagcgtt
     8821 tgggtgatga aggattattt aaggccgagt ggaaacaagc tctccctcag tttgtcagga
     8881 agatcggagt tattactagt ccaagtggag ctgttattcg ggatattatc accacagtca
     8941 gtcgtcgttt tcctggcgtg gaaatcttgc tttatccgac agttgttcag ggcgctggtg
     9001 cagcaccagc tatcgttgcc aatatccaaa aggctaataa cagaggagat ttagatctcc
     9061 tgatcgttgg tcgtgggggc ggttctatcg aggatctttg ggcttttaat gaagaaagcg
     9121 ttgtgcgggc cattttcgag tctcgtctgc caatcatttc gtctgtagga cacgagacgg
     9181 acacaacctt agcagacttc gtcgcagacc gccgagccgc aacgcctacg gctgcggctg
     9241 agctggcgac accagtgacc aaggcggatt tgcttaatca tctccgtcag caacaaaaca
     9301 gactttacac ggcatcactc aaccgcctac gtttttatca agaacgtctg cgcaagctga
     9361 gccaatcggt tatttttcgt cagccagaac ggctttacga cggctacctg caaaagctag
     9421 atcatctgac cgataagctc cagcagaata tgcaaaaagc ttaccagctg aatcaacagg
     9481 ctctgcgtct tttgcaagaa cggttaaaag cgcagcgttt ggatcaagaa ataaaacgcc
     9541 agcaggtcaa tttacagcaa cagacgcgtt tgctcaagag taacatggct atcctttatg
     9601 acaataaaat gaataaagtc aaacaactca gtgaggctat gctcatgctg gatacgaatc
     9661 gaattgtagc cagaggctat gccatggttt atcaaaaagg gcgcgttgtt gagagtgtta
     9721 agggagttgc aatcggctct caggctacca tccagatgcg ggatggtcag atagaagttg
     9781 aggtgaaaga tgtcaaagac gaaaaaaaca tttgaagata atttagcaga attggagacc
     9841 atcgtgggtc gtctagagtc aggtgatatt gctttggaag aggccttgtc tgaattccaa
     9901 aaggggatgg tcctctccaa ggaactccaa gcaacattag ccaaggcaga agaaaccttg
     9961 gtcaaggtca tgcaagaaga tggaacagaa gtagaattta atggatagac aaggcaaagt
    10021 ctcaggtgtt gaacaagcta ttcgatctta ttatgaggaa gcacaggtgg cagagcgtct
    10081 cgttgaggcg gttctctact ctgtcaatgc aggtggtaaa cgcattcgcc cactcttttt
    10141 cttggagatg gtcgaagctc tgggacagac gttgactcag gctcattttc aggtagcggc
    10201 aagtgtggag atgattcata caggtagcct tattcacgat gatctgccgg ccatggacaa
    10261 tgacgactac cgcagagggc gtttgaccaa tcacaagcag tttgatgagg caacagctat
    10321 tttggcgggc gacagtctct ttttggatcc atttggtctt attgcccgtg ctgattttcc
    10381 agcagaaata cgaattaatc tagtgcagga actgtctgac agctctggga catatggcat
    10441 ggtggctgga cagactttgg atatgatgtc tgagggcaaa ttgattgacc ttgagagctt
    10501 acagaccatt cacgccaata agacaggtaa gcttttgacc ttttctttcg tggcagctgg
    10561 tcttgtggcg gaggtttcta cggacgtctt gaccaaattg cgtcaggttg gagaactagt
    10621 cggactagcc tttcaggtgc gcgatgatat tttggatctt acggctgact ttgaggatct
    10681 cggaaaaacc ccgcagaagg atctcttggc ggagaagtcg acctaccctg ccttacttgg
    10741 tttagtagaa tcacagcagt tcttgaatca taccttggat caagttcaga atttgctcaa
    10801 ggaaattgct gaaaaatgcc cgtttcaggc aaagcaggtc ggtcaattga tagaaagttt
    10861 acgattaaat ggctaaagag cgtgtagatg tcctggcgca tcgtcaggga ctttttgaaa
    10921 ccagagagca ggctaagcgt ggggtcatgg caggtcttgt cattgccagt gtcaatggtc
    10981 accgctatga caagcctggt gagaagattg acgaagacac tgagcttaaa ctcaagggtg
    11041 aaaagctcaa gtacgtcagc cgaggtggcc tcaaacttga aaaggccctt gcgcaatttg
    11101 ggatttcagt ggcagaacaa ttgactttgg atattggagc gtcaacaggt ggttttaccg
    11161 atgtcatgct ccaggctgga gctaagatgg tctacgcggt tgacgtaggg actaatcagt
    11221 tagcttggaa gttgcgtcag gatccgcgag tgattagcat ggaacagtac aattttcgcc
    11281 atgctaaatt agatgatttt ccatatggac agccgatgtt tgcgtcaatt gacgtgagtt
    11341 ttatctcgct cagcctgatt ttgccagcac taacgcagat tttggcagat gacggtcagg
    11401 ttgttgcctt gattaaacct cagtttgagg caggacgcga gcaaataggc aaaaacggca
    11461 ttgtcaagga taaaaaagtg catttgcagg tgttagaaaa tgtgtctact tttgctcttg
    11521 agtcgggctt ttctgtccaa aacttagatt tttcaccgat ccaaggtggt catggaaata
    11581 ttgagtatct agctcatttg caaaaaagca aggcacagga aatggcttac agtcagtctc
    11641 tggctgagct tgtggagcaa tcacatcagg agtttaagca tgaataaaaa ggaacgtttg
    11701 cagattatca gcgatttgat cagtgagaat gcgattgata cccaagagga gattgtcaca
    11761 cgtttgcaag caattggcat taaggccacc caagcgaccg tttcgcgaga cattaagact
    11821 ctagggattg taaaggtgcc gtctgagtcg ggctatatct atgatctgcc tcactcacgg
    11881 aaacgtaaga ttagaccgca aaagaagtac cttcgcgatt ttgcggttca ggcaccattt
    11941 atccatctca aagttgagcc ggggacaagt gctgttgtca aacgtcagct gtctgagcaa
    12001 ttgcctgatc aactcttcag tgtaatagcc gatgatgata gtatcctgat ggtggtcaaa
    12061 gaaggtgcag cgattgaaga cgttcttgct aaagtcaagg agatttagat gttactagct
    12121 gtttcgatta agaattttgc aatcattgag caaatttcat tggacttcca gactggtatg
    12181 actgttttga ctggagagac cggtgctggg aagtccatta tcattgatgc catgaatatg
    12241 atgttaggag cgcgagcaac gactgatgcc attcgacatg gagctactaa ggctgaaatc
    12301 gaagggctct tttcgctgga aaaaagctct gccattgaca aaatcttgga agagcaaggc
    12361 attgaggttt caaacgagtt gattatccga cgggaaattt ttcaaaatgg ccggagtgtt
    12421 agtcgtatca atggccaaat ggtcaatctg acggttttga gagcggtcgg gcagcaccta
    12481 gttgacattc atggtcagca tgatcaggag gagctgatga agacacagtc ccacatcagc
    12541 atgctggatc agtttggaga tgaaggattt caggcacaaa aagctcagta tgaagaaact
    12601 tttgagcatt atcgtgtcct gcgccgtcag gttctgaccc agcaaaaaaa tgagcaagaa
    12661 aacaaggctc ggattgagat gttacgtttt cagctggacg agattgagtc agcagagcta
    12721 gaagcaggag aagacgagcg tcttcacaag gagcgagacc gcttggtcaa ccacaagaac
    12781 atcgcagata cgctggctaa tgcttttacc atgctagatg atgacgattt gggaaccttg
    12841 cgaaatttac gctcggctat gaatgacttg cagagtatcg aaagctacga acctgactat
    12901 aagaatatgg cagatcaggt ggtggagagt tactatgttt tggacgatgt cggtaagcgt
    12961 ctagggtcga ttttagataa tctggacttt gatggcaatc gtctcttgca ggtggagagt
    13021 cgtcttgatt tgctcaatag tatcacgcgt aagtatggcg ggagtgttga tgatgttttg
    13081 acgtactttc agaaaatttc cgaggaatac cagctcttga ccggcatttt gccggggcaa
    13141 ggcgatctgg aacaggagct caagcgtttg gaaaaagatc tgatcacgca ggcttcgcgt
    13201 cttagtcagg cgagacatga tttggctaag atattggagg cagaaatcaa gcaagagctt
    13261 cgagatctct atatggaaaa ggctgagttt caggtgacct ttaccaaggc taagtttagt
    13321 aaggttggca atgagcaggt ggaattctat atctcgacca accctggtga gggcttcaaa
    13381 ccgctagtca aggtggcatc cggtggcgaa ttatcccgtc ttatgctggc catcaagtca
    13441 gccttctcgc gcaaggagaa taagacgagt attgtctttg acgaggtgga tacgggagtt
    13501 tcaggccgtg tagcacaggc tattgcccag aaaatctaca aaatcgctca agctggtcaa
    13561 gtcttagcta tctcgcatct gccgcaggtt atcgctatcg ctgattatca attcttcatc
    13621 gaaaaaattt cagatgagac ctcaacagtc tctcgtgtcc gacttttaac gcttgaggag
    13681 cgcgtggaag aaatcgccaa gatgattgca ggagatagtt tgactccagc agcgcttgaa
    13741 caagcacgcg tgttgttgga gaaataaggt actcatgaag tacgatgagg ggattgtgtg
    13801 taacctctac atgccttttt gagatgaaag cacttcgctt atgaatacag cttttaaaaa
    13861 tgaaaatatc agagttgagt ctttttaaaa agaacttcat catcaatact aagctatctc
    13921 atggcgagta tcctccaaag cactataaat ttcaagtttg agtacatgaa atgaggctgg
    13981 acaaaaaatc caacctcatc tctcatagtt tacgtcaaca tctcagcgca gtggttggga
    14041 gtaagacttg ttggctatgc caacttagtc ttacccctgc aaagatcatt aggtgcttta
    14101 gcgccaatga accactgctg atgaggctct aatacatgga taatcaactt tagaacccta
    14161 atcaactgta cggggtggga aaacgaactc tttttgattg cttgagttct ttccactcct
    14221 attttaatgt tggctgaaca agacgaaaca gttgcatggc ctagccaagc tcttacacag
    14281 ttaggcaagc gagaccagcg aagcgctgaa tgcctccgaa aggactagtt ttttaaatca
    14341 atcctaaatg cttaaaggca ttgtagatac cgtcctcatc aacgcctgta gtgatgtaat
    14401 cagctgcttc cttagcttct gggccaccgc ttcccatggc aacgctataa gcgcagtaat
    14461 tgaacattgg gatgtcaatc ttggcgtcac caaaggcaaa tgtatctact tgtgctgctt
    14521 ggagatgact gagcaattgt tcaacggcaa aagccttgtc aatatctttg acaccgacat
    14581 ctccaaaaag agcaatttca cccttaccgc cccaagtgtt gacttcaaag ttggtgaaat
    14641 gagctttggc atctaaataa tcttggtaag attccaagat aaaggagatt ttgttaacat
    14701 cgtcacggta gaggtttgtc tcaccgaaaa caatcccgtg cagagcgtcc ttggtcatca
    14761 gagtttctgc attttcatat cctttacggc gagcgtactc gcgcatgaca gggagcgagc
    14821 gctcttcaaa gttttcagaa gcgtaaagtc cgctgttact ctcaaggtaa aattcaagac
    14881 cgtgctcatg gagatagtct acgatagcag tacagtcttc caaagttaga ctattgtgat
    14941 agacaacttc gccatcagac tcgatgtaat taccattgcc accaatcatg ccatcaatcc
    15001 ccatgtccca tagctcaggc tgcatctctg cacgtgagcg accggtacag gcataaactt
    15061 tgtggccatt tttacgggcc agctttatgg ctttgatggt ggaagctggg atttggtttt
    15121 cgtagttaac gatggttccg tcaacatcaa gaaatagaat tttagacata ggtcacctct
    15181 gtttgtttga ttactcttta attataaatg atattttagt cagtgtccga tattaaacgg
    15241 gggaaaactg atactatgct atgataattt tcaaaaaatc actagagtga gccaacaggt
    15301 agactgagcg agaatgcgct atctgtgata taatggaatt aatcaaatca aagcgagaat
    15361 acaaatgtac gttgaaatgc atgacgaaac aggtcaggtg actaaggcga ttcaggatca
    15421 gactttggct ttgctggatt ttgcggcgaa gcgtctagac aagcagcaaa aagaaatggc
    15481 tgtgactttc gtggacaatg ccagaatcca agagattaac aaactctatc gggataagga
    15541 tcagccgacc gatgtggtca gcttggagta caagccagac tctgaaattt tttttgagga
    15601 ggaagacttg gaagataacc ctgagctagc tgagatgatt gcggagttcg atagctatct
    15661 gggagaactt tacatctctg tcgataaggc tagggagcag gctgagagtt atggccatac
    15721 ctttgagcgg gaaatgggct tcttagcggt ccatggcttt cttcacatca atggctacga
    15781 ccactatact ccagaagaag aggccgagat gtttggcctc caagaagaaa ttttgagtgc
    15841 ttatggacta tcacgataaa aacaactctc gaaaaaaatg gaaaaaccgt gacctgatca
    15901 ccagtatgga atttgccatc acaggacttt tgacagcttt taaagaagag cgaaacatga
    15961 gaaagcatgg ggtatcagca gtactggtcg tcctcttagg cttactcttt caagtatcag
    16021 cgatcgaatg gctctttctg cttttagcgg ttagtctggt cattgccttt gaaatcgtca
    16081 attcagctat cgaaaatgtt gttgatttgg ctagtgacta tcacttttcc atgctagcca
    16141 agcacgctaa ggatatggca gcaggtgcag ttctttttgt ctcagggtat gccctcttga
    16201 ccggtgccat tatctttttg ccaaaagttt ggaacttgct tttttgaaaa aaataaaatg
    16261 aaaggatagg acagcccgtg ttctttaatc gaacacgaaa aatgctgagt gaagctaaga
    16321 taaaagtgtt aattgaacaa gacacagttg gagaggaacc tcgcttggtc tggctctgca
    16381 taattggtga gtagattgca gatcatgcgt attggcaggg tttgaaaagg aagtgtccga
    16441 cagtctggag agactgctga agtcactgaa atacctgctt tttgccttgc ttcagctggt
    16501 gacaaaaaat gctatcttgg cgagataaac ggtcacaaaa ccgagacgtt tctgctagtt
    16561 tcctgttttc acttggcttt tgctgtcctt tgtattctat gtttaaatca ggctttgtag
    16621 ctattttagg ccgaccaaat gtcggtaaat caacctttct taattatgtc atggggcaaa
    16681 aaattgctat catgagtgat aaggctcaga ccacccgtaa caaaatcatg ggaatttaca
    16741 ctactgaaac tgagcagatt gtctttattg acacaccagg gattcacaag cccaagacag
    16801 ctcttggcga cttcatggta gagtctgcct attcaaccct gcgtgaggta gagacggttc
    16861 tttttatggt gccggctgac gaggcgcgtg gtaagggaga tgatatgatt attgagcgct
    16921 tgaagaatgc taaagttccc gtgattttgg tcatcaacaa gattgacaag gtccatccag
    16981 atcagctctt ggagcaaatt gaagatttta aggaccagat ggacttcaag gagatcgtgc
    17041 cgatttcagc tactcagggg aacaatgtta accgactgat ggcaattctc aaagaaaacc
    17101 tccctgaggg gtatcaatat ttcccagaag accagattac agaccaccca gagcggttct
    17161 tagtatccga aatgattcgt gagaaagtgc tgcacctgac ccgtgaagaa atccctcact
    17221 ctgttgctgt tgtcgttgat tctatgaagc gcgacgaagc gactgataag gttcatattc
    17281 gtgcaactat tatggtagag cgcgacagcc aaaaggggat tatcattggg aaacaaggca
    17341 gcatgctcaa gaaaattggc tctatggccc gtcgtgatat cgaactcatg ctgggtgaca
    17401 aggtcttcct tgaaacctgg gttaaagtca aaaagaactg gcgtgacaaa aagctagact
    17461 tggcagattt tggctataat cagaaagaat attaagctgt gtgccaactc taccaatatt
    17521 tcgatagaaa atcggtgtga aaaaatgagt gagaaaaggg agattttctt taaattttat
    17581 gactagtttt tgttgctttg taatatttct gcctattcag tttaggaaaa ctaggagaga
    17641 atttcttcat acggttatcc tatgagtaga ctattatttg ttatcaaagc agtttttata
    17701 gtcttactca actgtgtgaa ctattgaagc atcactcctc agcatacgat tttttgtcta
    17761 ttcctacttt atttttagag gataaaacat gagacgcatt ataacagttc aacatacgga
    17821 gtctgttcac cataccaatg gtatgattgg gtcttggacc gattgggagt tgactgcgac
    17881 tggtaaagag caagcggagc gaattgctaa aaggttaaag gcagactatc cagatgttaa
    17941 ttttacattg tacagttctg atttgaagcg ggcgaagcaa acagctcaag ctataggaga
    18001 tgtttttggt attccaatcc atttgtcagg agccttacga gagcgaaatc tcggtagagc
    18061 ttgtgggcaa tcagtacagt ggctgaaaga taatatcgaa tctcatgaaa cggacattga
    18121 tagccggctt ttttcagatg ctgaaacgag acgagaagct tggaatcgtc tcaagccttt
    18181 ctatgatgag cttttgaatc aacctcatga aaatattatc ttagtctccc atggggattt
    18241 gctcagtctc tttaatgcta tgtggttagg attagaacct gaatttttga atcgtgctga
    18301 tttatatggc aattcaggtg gtgttagtct cttgcttgaa aaacaaggaa aacgtgttat
    18361 taaaagcatg agtgatatgt cttacattcg accgaaccaa gaatgatacc cttttgtttc
    18421 aagaaaagca tgctagatga cacgttgtct ttgtgaaact attgcccgaa ctttattcta
    18481 gcgtatcatg gaagataaga ttacagaatt gtaaaccaag aaaatgactg cccttgtaag
    18541 acagtcattt tcaatatcat aatattaggg gcaacttggt ttgaaaacag tatgcgatta
    18601 aaccagataa tggctttata ttagacttgt tcagaaactc attaaaaagt tgttctcttt
    18661 tcataaatta tcgttcaaga tttttaaatt gttatgacaa tcatcttgct tagatgatgg
    18721 agaagtctgt cttttaaaac attttcggtt tccgaacaag tctattgttt aaaaatggtt
    18781 ggcatatttg gcaaatggag atactatgat aatctgaaat agtcttttta cagttattga
    18841 taccttaaag gatattgtca aattcggcac gaacagcctt tggccagacg ctagattgaa
    18901 cttcgccgat atgtttttta cggagaagga acatggctag acgggattgg ccgataccgc
    18961 cgccaatgga cagcggaaag agaccgttga gcagggcacg atgccattct aattccagac
    19021 ggtcttcgtc tcccttaatg gcacactggc gtttgagggc gtcttcatcg acacggattc
    19081 ccatagaaga aagttcaaaa gcagatccca aagcctcatt ccaaacgagg atatcgccat
    19141 tgagcccttt gtaaccattt tcagttggag ttgtccagtc gtcatagtca ggagcacggc
    19201 catcatgcgg ttggccatcg gctagttcac caccgattcc gatcagaaag acagcgccaa
    19261 attctttggt gatggctttt tctcgctctt taggagtcaa atcaggatag cgctcgacca
    19321 gttcttctga atggataaat gtaatttgct ttgggaggac tgattcgatg tcatagcggg
    19381 cttcgacagc tagctcagtt aagcggatag ccttgtaaat caactcgaca gtttgtttga
    19441 ggtaggctag gttacgtttg ccatctggaa tgaccttttc ccagtcccac tgatccacat
    19501 aaatggaatg tgtcggatcc agatcgtcct cgtcaggtcg cagagctttc atgtggacaa
    19561 agagcccctc accttcatta aagccaaagc gggccagggt gtgtcgtttc cacttagcaa
    19621 gagaatgcac aacctcaaat gtatgctctg gaatgagctt gacatggacg gaaacgggat
    19681 tttcgacgcc tgagagattg tcctgcatgc catcgcccac acggctaagg atgggtccct
    19741 gaacttcaac gatttccaac ttatctttca aatattgggt aaaggtgttt ttgacaaaag
    19801 agatttcttc ttgctgatga ataaaacttt ttttcatagt ggtgtgtcct tcaataaaaa
    19861 ttaattctat tatagcgcta aatgaatgat atgaaaagta ttaatgaagg aagttttaaa
    19921 attttctgtc aataattgaa aaatcgtttt agaagttgaa agaagtacaa aacagtttta
    19981 agaaataaaa cctttgtgaa atatgctata ataaaaatca cagaactagg cgctttttag
    20041 cttatccaag gaggctttat gactcaagat tatatttcct atattcgctc taaggttggg
    20101 catgacaaga tcatattgaa ttttgcgggc ggtattcttg ctgatggaaa tggtagaatt
    20161 ttactgcaaa aacgcagtga ctttggcagc tacggcctcc caggaggagc gatggaattg
    20221 ggtgagagct caaaagaggc ttgtgagcgg gagttttttg aagagacggg gatcaaggtt
    20281 gaggccacgc gtcttctcaa cgtctacacc aaggatgaaa accattatcc caatggtgat
    20341 gaggtccaga ctgttgtcat cctctatgaa gtcaaagcgc ttgaggcgtt tgacatcaca
    20401 gattttcaca atggagaaac tttagaagtg ggcttctatt cggaggcaga aattgctgag
    20461 ctagagcctg tctttgacaa gcatagacga atgattgctg aatattttgc agacactttc
    20521 gcattgggga attagagagt tcatctagct gataacaaaa acacgataac caagaaggtt
    20581 tgcgatgagc agatcttctt ttttatttct gcaggataaa gttgacattt ttaaaaggat
    20641 tggacttgcc ggaacttggg tgatttccaa acaactcgaa cctgttttct cggttagcat
    20701 ttgtcattgt tctgcctgtt ttatgatatt ctagaggaaa tcatatcatc agtcgttaaa
    20761 ctgtctactt gctattttca aaatcgtctc caatcacctt ttcttcgcgt gtctgctagc
    20821 aaggcgcgag cttttacttt atggtataat gaaagttagc aagcaagtat aaggag
//
