LOCUS       NZ_FWXV01000002        10303 bp    DNA     linear   CON 09-DEC-2024
DEFINITION  Kibdelosporangium aridum strain DSM 43828, whole genome shotgun
            sequence.
ACCESSION   NZ_FWXV01000002
VERSION     NZ_FWXV01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Kibdelosporangium aridum
  ORGANISM  Kibdelosporangium aridum
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Kibdelosporangium.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2017) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900176515.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 16:02:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11,283
            CDSs (total)                      :: 11,193
            Genes (coding)                    :: 11,032
            CDSs (with protein)               :: 11,032
            Genes (RNA)                       :: 90
            rRNAs                             :: 3, 4, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1 (5S, 16S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 38 of 161
            Pseudo Genes (incomplete)         :: 135 of 161
            Pseudo Genes (internal stop)      :: 6 of 161
            Pseudo Genes (multiple problems)  :: 17 of 161
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:58:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1823427
            Orig. end                         :: 1833730
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FWXV01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10303
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10303
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10303
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5303](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5303
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    1647..1662
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.6"
                     /tool="antismash"
     tRNA            1752..1828
                     /anticodon=(pos:1825213..1825215,aa:Thr,seq:cgt)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="B9C43_RS18260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SAMN05661093_03721"
                     /product="tRNA-Thr"
     gene            1752..1828
                     /locus_tag="B9C43_RS18260"
                     /old_locus_tag="SAMN05661093_03721"
     gene            complement(2042..3313)
                     /locus_tag="B9C43_RS55635"
                     /old_locus_tag="SAMN05661093_03722"
     CDS             complement(2042..3313)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800123.1"
                     /locus_tag="B9C43_RS55635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05661093_03722"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_143446388.1"
                     /transl_table=11
                     /translation="MTGGRCDACGAELSRFATEPVCPTCRASAKHEPPSIPTPRLAPAV
                     WMWSAPEASIALRSRDLATILRIYRRLNRISQERLAALLGYDKTYVSMIETRRRIISDV
                     STRRHIARTLGLPTHVLGVTDTNDADFAAMLQFGDSTIRLAEIARQSGRAVDAVNELWP
                     LVARLEARAAEGHAERDTLILLGHGRAALGVSLGTVLPEERLTAAARWTGKALQVAERL
                     DDPVFLAHALRMHGNELRKADHLAAAVARLGRAVDVSTDREGQGAAYALLARAAGERGD
                     PDLFDYAISGYQDLLDNGTARGMLFNPFTFHEIQLRGLISTGRPNIAARIMETAQSDAL
                     PAAPQWHIIERVTAGQVLLAAGKRDAAEDALLTALASAEAHRLPHQIQRTIRAANEGGL
                     TDISDAGNAALGRLNDLLRPASSG"
     gene            complement(3318..3755)
                     /locus_tag="B9C43_RS18270"
                     /old_locus_tag="SAMN05661093_03723"
     CDS             complement(3318..3755)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020634944.1"
                     /locus_tag="B9C43_RS18270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05661093_03723"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_084427763.1"
                     /transl_table=11
                     /translation="MSQPDYVPRTFPVSIKGVVVRDGKVLLLKNERNEWELPGGRIELD
                     ETPEECVAREITEETQWKVTTGPILDTWMYYIDVAEKHVFIVTYGCYANGDTEPVLSHE
                     HKEIGLFPEHEVPTLNMPKGYKRSIATWYDRLREDTAELVG"
     gene            3882..4673
                     /locus_tag="B9C43_RS18275"
                     /old_locus_tag="SAMN05661093_03724"
     CDS             3882..4673
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: TauD (6-237). E-value: 1.9e-18. Score:
                     59.1. Matches aSDomain:
                     nrpspksdomains_B9C43_RS18275_TauD.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TauD"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014698.5"
                     /locus_tag="B9C43_RS18275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05661093_03724"
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="WP_084427765.1"
                     /sec_met_domain="TauD (E-value: 6.1e-17, bitscore: 59.1,
                     seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYRLDQFGEVGMVLDSLERYRVEVHGIPAALAGRGLALVEGLADS
                     SDLLRLAESIGTVVPHRDSGADGVTTIADLGTERVQSGFAGFSACALNPHTDRSGVDHP
                     PVLLMTSCGLPTATGGECVLIDGQAVYDDLAESEPDALRAFCAPRSVLFGGAAGYLGSI
                     FTDADDGRIEVRLRLDDLAQFSPEVCRWLPTLRAAIDRHAMMIKLDMGQGYVLNNHRWL
                     HGRRAFTGQRVIFRVNANPLPHLGIPFGFRPSPVAASSGMR"
     aSDomain        3900..4592
                     /aSDomain="TauD"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9C43_RS18275_TauD.1"
                     /evalue="1.90E-18"
                     /label="B9C43_RS18275_TauD.1"
                     /locus_tag="B9C43_RS18275"
                     /protein_end="237"
                     /protein_start="6"
                     /score="59.1"
                     /tool="antismash"
                     /translation="FGEVGMVLDSLERYRVEVHGIPAALAGRGLALVEGLADSSDLLRL
                     AESIGTVVPHRDSGADGVTTIADLGTERVQSGFAGFSACALNPHTDRSGVDHPPVLLMT
                     SCGLPTATGGECVLIDGQAVYDDLAESEPDALRAFCAPRSVLFGGAAGYLGSIFTDADD
                     GRIEVRLRLDDLAQFSPEVCRWLPTLRAAIDRHAMMIKLDMGQGYVLNNHRWLHGRRAF
                     TGQRVIFRV"
     misc_feature    4925..4945
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.69"
                     /tool="antismash"
     misc_feature    complement(4996..5010)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.8"
                     /tool="antismash"
     gene            5001..5303
                     /locus_tag="B9C43_RS18280"
                     /old_locus_tag="SAMN05661093_03725"
     CDS             5001..5303
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021219.5"
                     /locus_tag="B9C43_RS18280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05661093_03725"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_051897046.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.4e-11,
                     bitscore: 41.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNFKKFAVTGLSAAALIVGAASAATAAGSWQDGGWWEHGADAKWV
                     WSEYQHNQKCHSSAVDGANGVKGSGNKRPGDLAAVRDEAALFGNEAFWNSQAPCP"
     gene            5372..7414
                     /locus_tag="B9C43_RS18285"
                     /old_locus_tag="SAMN05661093_03726"
     CDS             5372..7414
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B9C43_RS18285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05661093_03726"
                     /product="hypothetical protein"
                     /protein_id="WP_084427767.1"
                     /transl_table=11
                     /translation="MSAKSLRFVLIANWIFACFFSFTLVTHWDELSPLPVESAVTVTQW
                     DRSKPANELHAQIEQFVMDRAITAGRVSNNFDGNGRTLYVAGSDPVVQQWLHGGYTDFT
                     RSVTTHVRPFADLGAQEPIGSYVVFGDYAKAVELGAFFSTQGMRVNERADRWSLALIDR
                     AVQDQQASVLKMMVLVSVAVAAAGVLLGARAYGVKRLQGFGYGGLLLSDLRRAATYWLA
                     AGALVLTVAAGFLAIYNGLASFGIYLLTALVISCVLAIVAIGTHALALALLMQLRVLAA
                     VKGELPARTASAAAYLLQAVTVVATVVLAERAIETGLDLERRADVSAAYTQLGSTSRIG
                     VGNVKSAVGFTEASSVVGAWLRAEEQANHLVLAGREIVQDAGALRGRDLMYVNENFLHA
                     QPIRLADGTRATTDGPALLIPADLWNHRDEVVRLLPSNLTTVDFKRAETAAGQEVFTYT
                     SEIDGPVAPSEVDKDHSFAKDPIVVFLPSRSGLITVDNLVAFATQGRVLFPNPQTVADA
                     VAADPELRRFVVSATPAAAEAAEERTDALRDFRLALFGVGAGMLVLFVSGIGAVLIHTR
                     RNAQLIFARHVSGWRFITTHRMFLAFEAVVLAVLVGWLPYQAARVNSRLAEYTSRGSSP
                     PFGPVTLGFPEWGAIGGLAVLTVGGVVAALIRAHRRVVRAGASEA"
     misc_feature    6479..6481
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            7426..8094
                     /locus_tag="B9C43_RS18290"
                     /old_locus_tag="SAMN05661093_03727"
     CDS             7426..8094
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.5"
                     /locus_tag="B9C43_RS18290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05661093_03727"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_084427769.1"
                     /transl_table=11
                     /translation="MLETRDLTMSYGERTLWTGLDLTLKPGQLTALTGPSGSGKSTLLN
                     CMGLLEQPTSGEIWFDGRELTRMRPGAVRRFRRDHLGYLFQNYALVENATIAENIDVAL
                     KVRGRRGNAAMSDALERVGLAGREDDRVSRLSGGEQQRVALARLIVKEPSLVLADEPTG
                     ALDHGNTEIVVSLLRNLADSGACVVIATHDDWVRDQCDETVSLEARGTASRVGVGAAPR
                     "
     gene            complement(8144..8737)
                     /locus_tag="B9C43_RS18295"
                     /old_locus_tag="SAMN05661093_03728"
     CDS             complement(8144..8737)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020632712.1"
                     /locus_tag="B9C43_RS18295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05661093_03728"
                     /product="class F sortase"
                     /protein_id="WP_084427771.1"
                     /transl_table=11
                     /translation="MHRKLMGGLIAATLTFAIAACGSSPAPPAAPPAPTSSAAVAAATT
                     EDGRAKPTNVRIPKIAAESTLVEAGLDKNKSIEVPPVEQPMQAAWYKYSQVPGDPGPAI
                     LLGHVDGNKKPGIFYKLHELKTGDEIFVKREDGKELRFVATRTEQVPKEKLPEEAIYGT
                     TQESELRLITCGGVFDKAAHSYKDNVIVYAKLAA"
     gene            complement(8737..9567)
                     /locus_tag="B9C43_RS18300"
                     /old_locus_tag="SAMN05661093_03729"
     CDS             complement(8737..9567)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B9C43_RS18300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05661093_03729"
                     /product="hypothetical protein"
                     /protein_id="WP_143446389.1"
                     /transl_table=11
                     /translation="MKKSMGLLSRAVLIGVSAAVLAAPAALATPATGTPETGAEQPQSD
                     VKERIAKYKKRVEQIEADLKDAVKAVDAATSKAEAQKAKAAAEKVKAAADALDKELQAF
                     LDSPDLKEHQDRIAVGQVMHRAHEASKAADAAITKAQKKIDDDQPTPPVPAADIAVEPQ
                     SAGRGGIVAIVVFCPEGKVSNFASDALNIVAESRFEDGPITAIGAIVKEDAAPGKHTVT
                     ASCDSQKLTTSFTVTADAPVQSKPPTSDDVRRKSVVKPKGKIETGGGATALQTV"
     misc_feature    complement(9658..9673)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
ORIGIN
        1 ctgactttcg ctgctgggct gaacccggcg gatccggcgc tggggcagcg ctgcgaccat
       61 gatgacgccg agcaacaaca gcgtcgtgcc cgtccagaac atcggaatgg cctggtaggc
      121 actggtgtac gcgagcggcg gctccttcgg cgcgggcgcc ttggcgggcg gcggcccacc
      181 cgcggtcccg cacaccacgc caccggtcgg cgcagcggcc cgcgcgacct gctcacccag
      241 ggacacctgc gccaacgccg acggaagacc cggcaggcca agcgcttgcg tcggcagcac
      301 ctgcaggtcg agcatgcgcg cgctggcggc gagctggtgg cccttgaacg gctgcgtcat
      361 gtcctcggcc ttctgctgca ggccggcgat ggacagccgg aaaacaccga tgtcgagctt
      421 gaactggttg agctcaccgg ccttgggcag cagcggcgag tccttcggca gcgcgttggc
      481 caagtcgccg atcagcggca gcttcttcat cgcgtccggc agttggttgc tcaagctggg
      541 cagcggaata ccgatcggga cgtcgaaagt cgggtgcgcg gcgtccagtg tgtacagaac
      601 cttgtcgccc tgcttgacct gcaggaccgg agcgacgtac ttgacggtcg aagtcttggc
      661 gtcaccggtg gacgtgacct gcagggtggg ctggctgacc acgttgatgg acatcgcgaa
      721 cggcgtgccg ccgagaaggt tgaggctggc gacctgaagc gtggacgtcg accgcacggc
      781 cttgttggca gaaccaggaa ggtcgacgag ctcgacgaca gaccgcgtgg acaacgtgtt
      841 gggcaggctg atcagcgacc cgtcaccctt gttggccggg cccggcaaca acccaccgag
      901 ctcagccaac ggcccaggca acgccttgag ctggtcggtc aacgcggtca gctggtcagg
      961 gctgagcttc gactcggtga gcttcccggt gaggtcattg gtgccgggaa gcgtcggaat
     1021 cgcgttcaac aggctcagac tggcaacgga cgtgctcgcg tccgacaacg gcgtgacaca
     1081 cgggccaagc gtctcgctcc accgcgcgtg cgcactgcca ttcatcaacc cgacccgaac
     1141 caacgcgtca agcggcgtct gcggcgggtt gagcccgaac gtcatcggct tctcgttgtc
     1201 cggcggagaa gtctgcgcga ccgcgcccgg cgactgcgga ctcttgccct gaacagcgac
     1261 accgccagga gaagcctgcg cgatggaatg ctcggtggcc aggtaagact cggagttgac
     1321 ctgagcgctg gacaacccga acccgacctc aagcgcggac tgcttgggca atttgtcgga
     1381 atagccgggc aggatcgtgt tggtcggagc ggaagcaggc agcaaccgga tcatcgaaag
     1441 agcggtcccg gcatccccga tcgcgtgtcc caacggctgc ggcccattgg gggcggaagc
     1501 aggcggcgcg ttcgggtcct tcggagcggg aatcggcgtg gtcgcaggcg gctgagccag
     1561 cgcggcagga gcagacacaa gcgcgagcac agccacaaca gccggcatag ccagggcgct
     1621 tctgcgcatg aacgaacccc tcctcgtgcg accgccgtca cacatccaac gaccggccag
     1681 ccgatcggtg actcacccga gcgggtggtc gtaaacgcct cgacggggcc cggtacactg
     1741 atcgaggaca cgccgcctta gctcagtcgg ccagagcgac gcactcgtaa tgcgtaggtc
     1801 aagggttcga ttcccttagg cggctccacc accagaccgc agttcacgtc gtgtgagctg
     1861 cggttttgtg tatcaggagc tgatctgctg gagaggcgcg ccgtctgatg cggcgacttg
     1921 tgagcccatg aggtcgggcg tgagcccgag gaaccagccg ggcttatcga catcgcggag
     1981 cagattttga gccaccgaga cgctgtcgac cggtcgcggc cagtccgcgc gcgcgggact
     2041 gtcagccgct ggaggctggc ctgagcagat cgttcaggcg cccgagggca gcgttcccgg
     2101 cgtcgctgat gtcggtgagt ccgccttcgt tggctgcgcg gatggtgcgt tggatttggt
     2161 gcggtagccg gtgtgcttcg gcggatgcca aagcggtgag aagagcgtct tcggcggcgt
     2221 ctcgtttgcc tgcggcgagg aggacttgcc cggcggtgac gcgctcaatg atgtgccact
     2281 gcggcgcggc aggcagagca tccgactgtg cggtctccat gatccgcgca gcgatgttgg
     2341 ggcggccggt ggagatcaga ccgcgtagct gtatttcgtg gaaagtaaag gggttgaaca
     2401 gcattccgcg ggcggttcca ttgtcgagga gatcctggta gccgctgatg gcgtagtcga
     2461 acaggtccgg gtcacctcgt tcgccagctg ctctcgcgag cagcgcgtaa gcagcgcctt
     2521 ggccttcgcg gtcagtagat acgtcgacgg ctcggccaag gcgtgcgacg gctgccgcca
     2581 gatggtctgc tttacggagt tcgttgccgt gcattcgtag ggcgtgggct aggaacaccg
     2641 gatcgtcgag gcgttcggcg acctgcaacg cttttccggt ccaccgtgcg gcggcagtga
     2701 gtcgttcttc gggcaatacg gtgccgagcg agacgccgag tgcagctctg ccgtggccga
     2761 gcaggatgag cgtgtctcgt tcggcatggc cctcggcggc gcgtgcctca aggcgggcaa
     2821 ccagaggcca tagctcgttt accgcgtcca ctgcccgacc ggactgccgg gcgatttcgg
     2881 cgagtcggat ggtggaatcg ccgaactgga gcatggcggc gaagtcggcg tcgttggtgt
     2941 cagtcacccc gagtacgtgg gttggcagtc caagagttcg agcgatgtgg cgtcgggtgc
     3001 tgacgtcact gatgatcctt cggcgggtct cgatcatcga cacgtaggtc ttgtcgtagc
     3061 ccagaagcgc cgcgagccgt tcttggctga tccggttgag ccgccgatag atgcgcagaa
     3121 ttgttgccag atcacggctt ctgagtgcga tgcttgcctc gggtgcggac cacatccaca
     3181 ccgcgggagc cagtcgaggg gtcgggatcg atggcggttc gtgcttggcg ctggcgcggc
     3241 aggtggggca aacaggctcg gtggcgaacc tgctcaactc cgcgccacac gcgtcgcacc
     3301 ttccgcctgt catggcacta tccgaccagt tccgcggtgt cttcgcgcag ccggtcgtac
     3361 caggtcgcga tcgagcgttt gtagcccttg ggcatgttca aagtgggcac ttcgtgttcg
     3421 gggaacaggc ctatttcctt gtgctcatgg gaaagcaccg gctcagtgtc gccgttcgca
     3481 tagcagccat aggtcacgat gaacacgtgc ttctcggcca cgtcgatgta gtacatccac
     3541 gtgtcgagga tcgggccggt ggtcactttc cattgggtct cttcggtgat ctcacgtgcg
     3601 acgcactctt cgggtgtctc gtcgagttcg atccggcccc caggcagttc ccactcgttc
     3661 cgctcgttct tgagcagcag cacttttccg tcgcgcacga ccacgccctt gatcgaaacg
     3721 ggaaacgtgc gcggaacgta gtcgggctgg ctcatcgagg cgtcctccga agtcgctgac
     3781 tagctgctta ccggcaacgt agcaccgcgg cactagctag gtgggcgacg gagggttgac
     3841 agtctgtcac tggacgtggc cgctcactcc ggttgtgctg gatgtaccgg ctcgaccagt
     3901 ttggggaggt aggaatggtg ctcgactcgc ttgagcgcta tcgcgtcgaa gttcacggga
     3961 taccggccgc gctcgctggg cgtgggctcg cattggttga gggtctggcc gactcgtcgg
     4021 acctgttgcg gttggctgag tcaatcggga cggttgtgcc gcatcgggac agcggagccg
     4081 atggcgtcac cacgattgcc gatcttggga ctgagcgcgt tcagagcggc ttcgccgggt
     4141 tcagtgcctg tgccctcaat ccgcacaccg atcgatcggg cgtcgaccat ccgcctgtgc
     4201 tgctgatgac gagctgcggc ctgccaacag ccacgggcgg cgaatgcgtg ttgatcgacg
     4261 gccaggcggt ctacgacgat ctcgccgagt ccgagccgga tgcgttgcgc gcgttttgcg
     4321 ctccccgctc cgtgttgttc ggcggcgctg ctggttacct cggttcgatc ttcactgatg
     4381 ccgatgacgg tcggattgag gtccgcctgc gattggatga cctcgcccag ttctcaccgg
     4441 aggtgtgccg ctggctgccg accttgcgag ctgcaatcga ccggcacgcg atgatgatca
     4501 agctggacat ggggcaaggg tacgtactga acaaccaccg ttggttgcat ggccgtcgcg
     4561 ccttcaccgg tcagcgcgtg atcttccgcg tcaacgcgaa ccctttgcca cacctgggga
     4621 ttccgttcgg tttccggccg agtccagtag cagcgtcgag cgggatgcgg tgacctgcca
     4681 tgtccgctcg ggcgaggccc gaagtgtgtc gcggatgtcc ggatcggtct cctgcaccgc
     4741 tgtcgtctgg ccgcatctgg ctacggtcgc cgcggtccat ccacaggctg agcctcgtct
     4801 ggttcctcag cttgtcctgt gtggacagtg tgggtggcgc gccggtcagt tgggaagtca
     4861 agcagagatt gaccacttaa tccctgcctg aaaccgctgt tctggttgac ggaaaatctc
     4921 taactttgtg tccggcagcc gacaacgccg gttgtccgcc ctgtttgttc aacgtatttc
     4981 gttagggttg attgaaaaac atgaatttca agaagtttgc tgtcacgggt ctttccgctg
     5041 ctgcgctgat tgtcggtgcc gcaagcgccg ccacggctgc aggctcgtgg caggacggcg
     5101 gctggtggga gcacggcgcc gacgccaagt gggtgtggtc ggagtaccag cacaaccaga
     5161 agtgccacag ctccgcggtc gacggcgcga acggagtcaa ggggtccggc aacaagcggc
     5221 cgggcgactt ggcggcggtc cgcgacgagg ccgcgctgtt cggcaacgag gccttctgga
     5281 acagccaggc cccgtgcccg tgagcctgag ccggtaaggg gttcccgctc cagccggagc
     5341 gggaaccttt ccccgcaagt gaggaaagtc tttgtccgcc aagagtctgc ggttcgttct
     5401 gatcgcgaac tggattttcg cctgcttttt ctcgttcacg ttggtgactc attgggacga
     5461 gctttcccca cttccggttg agtcggctgt cacggtcacg caatgggaca gaagcaagcc
     5521 ggcaaatgag ctgcatgccc agatcgaaca gttcgtgatg gaccgtgcca ttacggcagg
     5581 gcgggtgtcc aacaatttcg acgggaacgg ccgtacgctc tatgtcgccg gtagcgaccc
     5641 ggtagtgcaa cagtggctgc acggcggcta tacggatttc accaggtcgg tgaccacgca
     5701 tgtgcgcccg ttcgccgacc tgggcgctca ggagccgatc ggctcctacg tggtgttcgg
     5761 tgactacgcc aaggccgtcg agctgggcgc gttcttctct acgcagggca tgcgggtcaa
     5821 cgagcgggcc gaccggtgga gcctggccct catcgaccgg gcggtccagg accagcaggc
     5881 cagcgtcctg aagatgatgg tgctggtgtc ggtcgctgtg gccgctgccg gtgtgctgtt
     5941 gggagcgcgt gcgtacggcg tcaagaggct tcagggcttt ggttacggcg gtctgctgct
     6001 gtccgacctg cgccgtgccg ccacgtattg gttggccgcc ggtgcgctgg tcctcacggt
     6061 cgccgcgggt tttctcgcga tctacaacgg cctggcgagc ttcggtatct acctgctgac
     6121 cgcgttggtg atcagctgtg tgctggccat agtcgccatc ggcacgcacg ccctggcgtt
     6181 ggcactgctc atgcagttgc gcgtgctcgc cgccgtgaag ggcgaactgc ctgccagaac
     6241 cgcctccgcc gcggcttacc tgttgcaggc ggtgaccgtc gtcgccacag tggtcctcgc
     6301 cgagcgagcg atcgagacgg ggctggacct cgaacggcgt gccgacgttt ccgccgccta
     6361 cacgcaactt ggcagcacgt cgcggatcgg tgtgggcaac gtcaagtcgg ctgtcggctt
     6421 cacagaggcc agttcggtcg tcggtgcgtg gcttcgtgcc gaggagcagg cgaaccactt
     6481 agtcctcgcg ggacgtgaaa tcgtccagga cgcaggtgcg ctgcgtggcc gagacctgat
     6541 gtacgtcaac gagaacttcc tgcacgccca gccgattcgc ctggctgacg ggacgagggc
     6601 cacgacggat gggccggctc tgctcatccc ggcggacctg tggaaccacc gcgacgaggt
     6661 ggtccgccta ctgcccagca atctgaccac agtggacttc aagcgggccg aaacagcagc
     6721 gggccaagag gttttcacct acacatccga gatcgacggg ccggtggcgc cgagtgaggt
     6781 cgacaaggac cactccttcg ccaaggaccc catcgtggtc ttccttcctt cgcgaagcgg
     6841 actgattact gtggacaacc tggtcgcctt cgcaacgcag ggacgcgtac tgttcccgaa
     6901 tccacagaca gtcgcggacg cggtggccgc cgaccccgaa cttcgccggt tcgtcgtgtc
     6961 ggcgacccct gcggcagccg aggccgccga agagcgaacc gacgcgctgc gcgacttccg
     7021 gctggcactg ttcggagtcg gggcgggcat gctggtgctg ttcgtcagcg ggatcggtgc
     7081 ggtgctgatc catactcggc gcaacgcaca gctcatcttc gcgaggcacg tgagcggctg
     7141 gcggttcatc acgacacacc ggatgttcct ggccttcgag gcggtcgtgc tcgcagtcct
     7201 ggtcggttgg ttgccgtacc aggctgcacg cgtcaacagc cgcctcgcgg agtacacgag
     7261 ccgaggttcg tccccgccgt tcggccctgt gacgcttggc tttccggaat ggggcgccat
     7321 cggcgggctc gccgtactga ccgttggcgg cgtggtcgcg gctctgattc gcgcgcaccg
     7381 ccgggtagtc cgcgctggag ccagtgaagc gtaatggagg caacaatgct ggaaacccgc
     7441 gacctcacca tgtcctacgg cgagcggaca ctgtggactg gcctcgacct gacgttgaag
     7501 ccgggccagt tgacggcact gaccggccca agtggatccg gaaagtccac tttgctcaac
     7561 tgcatggggt tgctggaaca gccgaccagc ggcgagatct ggttcgacgg gcgtgaactg
     7621 acgcggatgc ggcccggtgc ggttcgccgg ttccgccgtg accacttggg ttacctgttc
     7681 cagaactacg cgctcgtgga gaacgcgacc atcgccgaga acatcgatgt ggcgctgaag
     7741 gtccgcggcc gtcgcggcaa cgcggcgatg tcggacgccc tggagcgagt ggggctggcc
     7801 ggccgtgaag acgaccgggt cagccggctt tccggcggtg aacagcaacg ggtcgcgttg
     7861 gcccgtctca tcgtgaagga accgagcctg gtgctggccg acgaaccgac cggcgccctt
     7921 gatcacggca acacggagat tgtcgtttcg ttgctgcgca atctggcgga cagcggtgcg
     7981 tgcgtggtca tcgccacgca cgacgactgg gtccgtgacc agtgcgacga gaccgtctca
     8041 ttggaagcgc gagggacagc ctcgcgcgtc ggtgtcggcg ccgcaccgcg ataagcagca
     8101 agaagcccgg gccgctgtga ggcccgggcg ttctggtgct tggttacgcg gctagcttcg
     8161 cgtagacgat gacgttgtcc ttgtagctgt gagctgcttt gtcgaagact ccgccgcagg
     8221 tgatcaggcg tagctctgac tcctgcgtcg tgccgtagat ggcttcttcg ggcagttttt
     8281 ccttcgggac ctgctctgtg cgggtcgcta cgaaacgcag ttctttgccg tcttcgcgct
     8341 tcacgaagat ctcgtcgccg gttttcagct cgtgcagctt gtagaagatt cccggcttct
     8401 tgttcccgtc gacgtggcca agcaggatcg ccgggccggg gtcgccgggg acttggctgt
     8461 acttgtacca agctgcttgc atcggctgct cgaccggtgg gacttcgatc gatttgttct
     8521 tgtccaggcc cgcttcgacc agtgtcgact ccgcggcgat cttggggatt cgcacgttcg
     8581 tcggcttcgc ccgtccgtcc tccgttgtcg ctgccgcgac ggcagcggag gacgtggggg
     8641 cgggtggcgc agcgggaggc gccggggatg agccgcatgc ggcgatggcg aaggtgaggg
     8701 tggccgctat gaggccaccc atcagcttgc ggtgcatcag accgtctgca gtgcggtcgc
     8761 gccgccgccg gtctcgatct tgcccttcgg cttgacgacg ctcttgcggc gcacgtcatc
     8821 gcttgtcggc ggcttcgact ggaccggtgc gtccgcggtc acggtgaacg acgtggtcag
     8881 cttctgcgag tcgcacgagg ccgtgacggt gtgcttgccg ggtgccgcgt cctctttgac
     8941 gatcgcgccg atcgcggtga tcgggccgtc ctcgaagcgt gactcggcaa cgatgttcag
     9001 cgcgtcggag gcgaagttgc tcaccttgcc ctcggggcag aacacgacga tcgcgacaat
     9061 gccgccgcgg ccggcggact gcggctccac ggcgatgtcc gcggcgggca cgggcggcgt
     9121 cggctggtcg tcgtcgatct tcttctgcgc cttggtgatc gccgcgtccg cggccttgct
     9181 cgcctcgtgg gcgcggtgca tgacctgacc gaccgcgatc cggtcctggt gctccttcag
     9241 gtcagggctg tccaggaacg cctgcagctc cttgtcgagc gcgtccgccg cggccttgac
     9301 cttctcggcc gctgccttcg ccttctgcgc ctcggctttg ctggtcgccg cgtcgaccgc
     9361 cttcactgcg tccttcaggt cggcttcgat ctgctcgacg cgcttcttgt acttggcgat
     9421 ccgctctttc acgtccgact gcggctgctc ggcgccggtc tccggcgtgc cggtggccgg
     9481 ggtggccagt gctgccgggg ctgccagcac ggcggcggaa accccgatga ggacagcgcg
     9541 cgacagcagg cccatcgact tcttcaagac tttctcccct tgttacgcat aacagcgaaa
     9601 atcccccgaa tgccgctcag ctaacccgag gacggcgttt tcgccaagcc ggttatgcgt
     9661 gaacaacctc accggaagaa cctgtaacaa aaaccggatc ccaaggtcag gggcaaaatt
     9721 ttacgcaacc tcgtcacggc gcgtgcttac tatcttcgcc ttccgcagtc gtcctcgcaa
     9781 tagcccgttg gcgtggcaca agatcacagc ggtgaaggcg aggcagagcc agctgaacca
     9841 gtcgccgaag cgggtataga cggtcgcgcc gggacctagt ggaacgtctg aaattacagt
     9901 cgtgaagcca gtgctgcccg tgggctcgga tgcgatcacg cggccatagc catcagcgag
     9961 catcaggttg gtttgcctgc cgccccaagc gatggcgacg ccgttctcca cgccgcgaag
    10021 cagggcggtg cgactgtgct gccagccgtt ttcgtcttcg tccgaggcgg gaatggctaa
    10081 cagccgagcg cctgcggcgg cgtaggtggc gctgggattg cggtggttta cgtccataca
    10141 gattgcgatg cccacatgcg gtttggcgaa tgtcagggaa tcacctggcg ggctcacggt
    10201 gtcgtggtgc ttgagatatc gggccgctcc cgggaacgtc aacgcgtagt tgtacttcgc
    10261 gtctgtccat tccgcgtagc cgaccacgat ggtgaagcgc tgc
//
