LOCUS       NZ_FUWZ01000024         2276 bp    DNA     linear   CON 22-OCT-2024
DEFINITION  Chitinophaga eiseniae strain DSM 22224, whole genome shotgun
            sequence.
ACCESSION   NZ_FUWZ01000024
VERSION     NZ_FUWZ01000024.1
KEYWORDS    WGS; RefSeq.
SOURCE      Chitinophaga eiseniae
  ORGANISM  Chitinophaga eiseniae
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Chitinophaga.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2017) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900167135.1-RS_2024_10_22
            Annotation Date                   :: 10/22/2024 16:06:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,843
            CDSs (total)                      :: 6,771
            Genes (coding)                    :: 6,741
            CDSs (with protein)               :: 6,741
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:38:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2276
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FUWZ01000024.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2276
                     /db_xref="taxon:634771"
                     /mol_type="genomic DNA"
                     /note="contig: Ga0070509_124"
                     /organism="Chitinophaga eiseniae"
                     /strain="DSM 22224"
                     /type_material="type strain of Chitinophaga eiseniae"
     gene            <1..>2276
                     /locus_tag="B5D63_RS34460"
                     /old_locus_tag="SAMN04488128_1241"
     region          1..2276
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2276
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2276
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:>2276](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..>2276
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             1..>2276
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (184-253). E-value: 4.1e-24. Score:
                     76.6. Matches aSDomain: nrpspksdomains_B5D63_RS34460_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (268-569). E-value:
                     1.4e-100. Score: 328.1. Matches aSDomain:
                     nrpspksdomains_B5D63_RS34460_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="B5D63_RS34460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488128_1241"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_143313389.1"
                     /sec_met_domain="AMP-binding (E-value: 9.9e-39, bitscore:
                     119.0, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 6.4e-14, bitscore:
                     38.6, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 3.7e-79, bitscore:
                     252.0, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDRLQLCAVGITGEICIGGAGLARGYLGRPELSAEKFVPDPYRAG
                     ERLYRTGDLGRWLPDGTIAFAGRRDDQVKVRGYRIELGEIVHALEQHPGISAAVVITQG
                     ATAEEKTLVSYFTGDAGLDIATLRAYLDKRLPAYMLPDYYIHLDTLPLNASGKVDRKRL
                     PTPQGAGLATGREYVAPRNTTEEKLVDIWRNVLGRTGIGVKDSFFELGGHSLKATRLES
                     QIYKVFEVKVALHDLFGAPVLENQAALIERSRKTVYNSIMPAPKQSGYPLSSSQRRLWV
                     LSQFAESSVAYNMPGAYVFEGVLAISSLTYAFNELIRRHEILRTVFNEDENGEVKQYIL
                     EADESIFNIEYVDLRDIANRKARLQQLVERAAGKAFDLSRGPLLRAGLYQTDDHRWIFA
                     YTMHHIISDGWSMDVLLRELLHIYNVHTAGQPHALQGLSVQYKDYAFWQQCRLTDGSLA
                     TAQAYWLKQFEGELPQLLLPLDHSRPAVKTYNGGVVVKLLPASLSGALKLLVKEQGATL
                     FMGLFGLVNVLLHRYTQQEDLIIGTPIAGREHADLSDQIGIYINTLALRTRFSGNENFV
                     SLLKQIRENMLAAYEHQAYPFDELVEQLQLQRDMSRNALFDVMVILQNNERRVLSGDGI
                     LNNLHLSAYENHTKEISKLDITFEFEETEEGIVIYLKYNSDLFEAATIHRMAAHLQQLL
                     KAALHTPDMAVNSLPYLTEGEESALLDIFNVKSPGYDTGATIITCFEQQVAQSAERTAL
                     VFKDSS"
     CDS_motif       25..111
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34460_0001"
                     /evalue="4.80E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="B5D63_RS34460"
                     /protein_end="37"
                     /protein_start="8"
                     /score="50.8"
                     /tool="antismash"
                     /translation="VGITGEICIGGAGLARGYLGRPELSAEKF"
     CDS_motif       193..255
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34460_0002"
                     /evalue="1.60E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="B5D63_RS34460"
                     /protein_end="85"
                     /protein_start="64"
                     /score="36.1"
                     /tool="antismash"
                     /translation="FAGRRDDQVKVRGYRIELGEI"
     aSDomain        553..759
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B5D63_RS34460_PCP.1"
                     /evalue="4.10E-24"
                     /label="B5D63_RS34460_PCP.1"
                     /locus_tag="B5D63_RS34460"
                     /protein_end="253"
                     /protein_start="184"
                     /score="76.6"
                     /tool="antismash"
                     /translation="EEKLVDIWRNVLGRTGIGVKDSFFELGGHSLKATRLESQIYKVFE
                     VKVALHDLFGAPVLENQAALIERS"
     aSDomain        805..1707
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B5D63_RS34460_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.40E-100"
                     /label="B5D63_RS34460_Condensation_LCL.1"
                     /locus_tag="B5D63_RS34460"
                     /protein_end="569"
                     /protein_start="268"
                     /score="328.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="GYPLSSSQRRLWVLSQFAESSVAYNMPGAYVFEGVLAISSLTYAF
                     NELIRRHEILRTVFNEDENGEVKQYILEADESIFNIEYVDLRDIANRKARLQQLVERAA
                     GKAFDLSRGPLLRAGLYQTDDHRWIFAYTMHHIISDGWSMDVLLRELLHIYNVHTAGQP
                     HALQGLSVQYKDYAFWQQCRLTDGSLATAQAYWLKQFEGELPQLLLPLDHSRPAVKTYN
                     GGVVVKLLPASLSGALKLLVKEQGATLFMGLFGLVNVLLHRYTQQEDLIIGTPIAGREH
                     ADLSDQIGIYINTLALRTRF"
     CDS_motif       871..981
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34460_0003"
                     /evalue="1.30E-14"
                     /label="C2_LCL_024-062"
                     /locus_tag="B5D63_RS34460"
                     /protein_end="327"
                     /protein_start="290"
                     /score="46.5"
                     /tool="antismash"
                     /translation="AYNMPGAYVFEGVLAISSLTYAFNELIRRHEILRTVF"
     CDS_motif       1204..1269
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34460_0004"
                     /evalue="4.70E-12"
                     /label="C3_LCL_132-143"
                     /locus_tag="B5D63_RS34460"
                     /protein_end="423"
                     /protein_start="401"
                     /score="38.3"
                     /tool="antismash"
                     /translation="MHHIISDGWSMDVLLRELLHIY"
     CDS_motif       1318..1353
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34460_0005"
                     /evalue="1.60E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="B5D63_RS34460"
                     /protein_end="451"
                     /protein_start="439"
                     /score="14.3"
                     /tool="antismash"
                     /translation="QYKDYAFWQQCR"
     CDS_motif       1513..1548
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34460_0006"
                     /evalue="5.20E+01"
                     /label="C3_dual_135-156"
                     /locus_tag="B5D63_RS34460"
                     /protein_end="516"
                     /protein_start="504"
                     /score="-2.6"
                     /tool="antismash"
                     /translation="ALKLLVKEQGAT"
     CDS_motif       1609..1698
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34460_0007"
                     /evalue="9.20E-12"
                     /label="C5_LCL_267-296"
                     /locus_tag="B5D63_RS34460"
                     /protein_end="566"
                     /protein_start="536"
                     /score="37.4"
                     /tool="antismash"
                     /translation="DLIIGTPIAGREHADLSDQIGIYINTLALR"
     CDS_motif       1747..1860
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34460_0008"
                     /evalue="9.70E-20"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="B5D63_RS34460"
                     /protein_end="620"
                     /protein_start="582"
                     /score="63.0"
                     /tool="antismash"
                     /translation="RENMLAAYEHQAYPFDELVEQLQLQRDMSRNALFDVMV"
ORIGIN
        1 gacgaccgtt tgcagctgtg cgcagttggt atcaccggtg aaatatgtat aggaggcgca
       61 ggcctggcgc ggggctacct gggccgtcct gagctgagcg cggagaagtt tgttcctgat
      121 ccttaccggg ccggcgaacg gctgtaccgc acaggcgacc ttggccgctg gctgccagat
      181 ggaacgatcg cttttgcagg tcgccgcgac gaccaggtaa aggtgcgtgg ttaccgtatt
      241 gagctggggg agatagtgca tgccctggaa cagcaccccg gtatttcggc ggccgtggta
      301 ataacacagg gcgcaacggc tgaagaaaag acattggtaa gttacttcac cggtgacgcc
      361 gggctggaca tcgccacttt acgggcttac cttgacaagc gcctgcccgc ctacatgctc
      421 ccggactatt atatacacct ggatacatta ccgttgaatg ccagcgggaa ggtggacagg
      481 aaacggctgc caactccgca gggcgccggc cttgcaaccg gcagggaata tgttgcgccc
      541 cgaaatacca ccgaagaaaa gctggtggac atctggagaa acgtgctggg gcgtaccggc
      601 attggcgtaa aagatagctt ttttgagttg ggtggtcata gtttaaaggc cacgcgtctc
      661 gaaagccaga tatataaagt atttgaggta aaagtagctt tgcacgatct gtttggtgcg
      721 ccggtattgg aaaatcaggc cgccctgata gaacgttccc ggaaaactgt ttataacagt
      781 atcatgccgg ccccgaaaca atcaggctac ccgctgtctt cttcacagcg tcgtttatgg
      841 gtattaagtc agtttgccga aagcagcgtc gcttacaata tgcccggagc gtatgtgttt
      901 gaaggagtcc ttgccatatc ctctttaacc tatgccttta acgaattaat acgccgtcat
      961 gagatcctgc gcacggtatt taatgaagat gagaacggag aagtaaagca gtatattctg
     1021 gaggcggatg aaagcatttt caacattgaa tatgttgacc tgcgtgatat cgccaaccgg
     1081 aaggcgcgtt tgcagcagtt ggtggaacgc gcagcgggaa aagcatttga cctgtccagg
     1141 gggccgctgt taagggccgg actttaccag acagatgatc atcgctggat atttgcctat
     1201 accatgcatc atattataag tgatggctgg tcaatggacg tactcctccg cgagctgctg
     1261 catatctaca atgtacacac ggcaggccag ccccatgcct tgcagggctt gtctgtccag
     1321 tataaggatt atgctttctg gcagcagtgc cggttaacgg atggctcact ggctacagca
     1381 caggcctact ggctgaagca atttgaagga gagctgccgc agctgctgtt gccgctggac
     1441 cattcccgtc ccgctgtcaa aacgtataat ggaggcgtgg tggtaaaact gctccctgcc
     1501 agtctgtccg gcgctttgaa gctgctcgtg aaagagcagg gcgccacact gtttatgggg
     1561 ctgtttgggc tggtgaatgt attgctgcat cggtacacac aacaggaaga tcttattatc
     1621 ggtaccccga tcgctggcag ggagcatgca gatctttccg accagatagg tatttatata
     1681 aatacactgg ccttgaggac aaggtttagc ggaaatgaga attttgtcag cctgcttaaa
     1741 cagatacggg aaaatatgct ggctgcctac gagcaccagg catatccttt tgatgaattg
     1801 gtggaacagc tgcagctgca gcgggatatg agccgtaatg cgctgttcga tgtaatggtt
     1861 atactgcaga ataatgaacg ccgggtgcta agtggggatg gtatcctaaa taacctgcat
     1921 ttaagcgcat atgaaaacca tacaaaggag ataagcaagc tggatattac ttttgagttc
     1981 gaggaaacag aagaaggcat tgtcatctac ctgaaatata atagcgacct gtttgaggca
     2041 gcgaccatcc atcgcatggc agcgcatctt cagcaattgt tgaaagcagc gctgcatact
     2101 cctgacatgg cggtaaacag cttgccttat ttaacggaag gagaggagtc ggcgctgctg
     2161 gacatattca atgtaaaatc gcccggctat gatacaggag cgacgatcat cacctgcttt
     2221 gaacaacagg tggcgcagtc ggctgaacgc acagcccttg tctttaaaga cagctc
//
