LOCUS       NZ_FUWZ01000022         3594 bp    DNA     linear   CON 22-OCT-2024
DEFINITION  Chitinophaga eiseniae strain DSM 22224, whole genome shotgun
            sequence.
ACCESSION   NZ_FUWZ01000022
VERSION     NZ_FUWZ01000022.1
KEYWORDS    WGS; RefSeq.
SOURCE      Chitinophaga eiseniae
  ORGANISM  Chitinophaga eiseniae
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Chitinophaga.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2017) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900167135.1-RS_2024_10_22
            Annotation Date                   :: 10/22/2024 16:06:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,843
            CDSs (total)                      :: 6,771
            Genes (coding)                    :: 6,741
            CDSs (with protein)               :: 6,741
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:38:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 3594
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FUWZ01000022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3594
                     /db_xref="taxon:634771"
                     /mol_type="genomic DNA"
                     /note="contig: Ga0070509_122"
                     /organism="Chitinophaga eiseniae"
                     /strain="DSM 22224"
                     /type_material="type strain of Chitinophaga eiseniae"
     gene            <1..>3594
                     /locus_tag="B5D63_RS34445"
                     /old_locus_tag="SAMN04488128_1221"
     region          1..3594
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..3594
                     /SMILES="NC(CCC(=O)N)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..3594
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[2:>3594](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3..>3594
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             3..>3594
                     /NRPS_PKS="Domain: AMP-binding (280-685). E-value: 2.5e-88.
                     Score: 288.3. Matches aSDomain:
                     nrpspksdomains_B5D63_RS34445_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (788-854). E-value: 1.3e-24. Score:
                     78.2. Matches aSDomain: nrpspksdomains_B5D63_RS34445_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (866-1157). E-value:
                     4.7e-123. Score: 403.4. Matches aSDomain:
                     nrpspksdomains_B5D63_RS34445_Epimerization.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /locus_tag="B5D63_RS34445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488128_1221"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_143313388.1"
                     /sec_met_domain="AMP-binding (E-value: 7.3e-102, bitscore:
                     327.0, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.4e-16, bitscore:
                     45.5, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 6e-65, bitscore:
                     205.3, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAMQEQHRSYWKSVFADGVPLLHLPLDYKRPLIKDDKGGVVRFSL
                     SREEVSALQALASRSQSTLFMVMLSLYNVLLSRVCNQDDIVVGTSVSGRYHADLEPLTG
                     MFVNTLPLRHHPHGDQSFLSFLTTVRDRTLESFEHQSYPLEAMVEDLSPERDTSRSPLF
                     DVMFGYENIDVTTLAIPGLKLEPYLHEEYASKFDLTLTVSERGDDLQLDFEYAASLFNS
                     STVHRLITYFKNIVRAVIADNEVLLSAIVMLPEAEEHTLLHVFNNTARSYGNTTFPDVF
                     KDQAALYPHHIAATYRGVDTDYRTLHETSNRMAHYLHSIGLPAGAVVPLLLDRGVDLLS
                     WIIGIQKAGGVFIALDTRHPADRIAGILDGCNPALVVTEEKYTGLLKAGCPMVLHETVA
                     TTAHDRSVSDWMSRKAADIAYAAYTSGSTGVPKGVLLHHGGMMNHFHGLKELLGLTSDD
                     RFAQTAECSFDVYLVQMLLALTVGGCTHIIDRDVMLDAGLLSAELVAGDITILEVVPSV
                     VRLLVNAAPLPLGKLRWVISTGEALSRELAVAWYRCYPDVRLLNLYGPAETSDDATYYI
                     VPPDLQEQGVGNVPIGQPLPNFHIHVLDAARNLCPVGVSGEICIAGPGVGKGYIADVSL
                     TASKFVPNPFASHDTSDAYAVMYRTGDVGYRDEQGMIYLTGRIDNMIKIRGARIETGDI
                     EALLLQFAGIRDVLVLSRENKEQQYLAAYYIAEGEIDAAVLAAYLTGKLPEYMVPTAYV
                     HLTAFPLTLSGKVNKRELPEPVFGSEAFQAPVTDEEILLAGIWCKILGMARVGTADNFF
                     SLGGDSIKSIQIISRVRAAGYELSMKDIFANQTIAGLARCLKVLRAQPRQDIVKGEAAL
                     TPVQRFFFDGPAAGDAHFNQSVMLHFPQGITAGEVQQIFGYLQQHHDALRIVIKETAEG
                     LRLDTKPEQPVSLTVYDAYNEAACTALQGSMDLTAGPLMKLGLFREKDGSHLLIVVHHL
                     VTDGVSWRILLEDMATLYGQLQQKQTLSLPGKTTPFLSWSAHLHHYMESASFRSGRAYW
                     QAQEKRGLQGFIPRDMPEGSNRGEEMRTVRFTLDSMRTHELLRQAPDTFRTSVEEVLLA
                     AFVAAVAQQYGQQQVCVDLEGHGREEILPGVDISRTVGWFTSIYPVMLELKGTTWSDRL
                     RHVKETLRTIPNKGIDYLLCRYYDNSLADAEA"
     CDS_motif       253..342
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34445_0001"
                     /evalue="6.50E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="B5D63_RS34445"
                     /protein_end="113"
                     /protein_start="83"
                     /score="44.2"
                     /tool="antismash"
                     /translation="DIVVGTSVSGRYHADLEPLTGMFVNTLPLR"
     CDS_motif       391..510
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34445_0002"
                     /evalue="3.20E-19"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="B5D63_RS34445"
                     /protein_end="169"
                     /protein_start="129"
                     /score="61.3"
                     /tool="antismash"
                     /translation="RDRTLESFEHQSYPLEAMVEDLSPERDTSRSPLFDVMFGY"
     aSDomain        844..2058
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B5D63_RS34445_AMP-binding.1"
                     /evalue="2.50E-88"
                     /label="B5D63_RS34445_AMP-binding.1"
                     /locus_tag="B5D63_RS34445"
                     /protein_end="685"
                     /protein_start="280"
                     /score="288.3"
                     /specificity="substrate consensus: Gln"
                     /tool="antismash"
                     /translation="FKDQAALYPHHIAATYRGVDTDYRTLHETSNRMAHYLHSIGLPAG
                     AVVPLLLDRGVDLLSWIIGIQKAGGVFIALDTRHPADRIAGILDGCNPALVVTEEKYTG
                     LLKAGCPMVLHETVATTAHDRSVSDWMSRKAADIAYAAYTSGSTGVPKGVLLHHGGMMN
                     HFHGLKELLGLTSDDRFAQTAECSFDVYLVQMLLALTVGGCTHIIDRDVMLDAGLLSAE
                     LVAGDITILEVVPSVVRLLVNAAPLPLGKLRWVISTGEALSRELAVAWYRCYPDVRLLN
                     LYGPAETSDDATYYIVPPDLQEQGVGNVPIGQPLPNFHIHVLDAARNLCPVGVSGEICI
                     AGPGVGKGYIADVSLTASKFVPNPFASHDTSDAYAVMYRTGDVGYRDEQGMIYLTGRID
                     NMIKIR"
     aSModule        844..3474
                     /complete
                     /domains="nrpspksdomains_B5D63_RS34445_AMP-binding.1"
                     /domains="nrpspksdomains_B5D63_RS34445_PCP.1"
                     /domains="nrpspksdomains_B5D63_RS34445_Epimerization.1"
                     /locus_tags="B5D63_RS34445"
                     /monomer_pairings="Gln -> D-Gln"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       1258..1317
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34445_0003"
                     /evalue="5.50E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="B5D63_RS34445"
                     /protein_end="438"
                     /protein_start="418"
                     /score="25.1"
                     /tool="antismash"
                     /translation="AYAAYTSGSTGVPKGVLLHH"
     CDS_motif       1834..1923
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34445_0004"
                     /evalue="9.60E-12"
                     /label="NRPS-A_a6"
                     /locus_tag="B5D63_RS34445"
                     /protein_end="640"
                     /protein_start="610"
                     /score="37.1"
                     /tool="antismash"
                     /translation="PVGVSGEICIAGPGVGKGYIADVSLTASKF"
     CDS_motif       2026..2088
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34445_0005"
                     /evalue="5.50E-08"
                     /label="NRPS-A_a8"
                     /locus_tag="B5D63_RS34445"
                     /protein_end="695"
                     /protein_start="674"
                     /score="25.2"
                     /tool="antismash"
                     /translation="TGRIDNMIKIRGARIETGDIE"
     aSDomain        2368..2565
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B5D63_RS34445_PCP.1"
                     /evalue="1.30E-24"
                     /label="B5D63_RS34445_PCP.1"
                     /locus_tag="B5D63_RS34445"
                     /protein_end="854"
                     /protein_start="788"
                     /score="78.2"
                     /tool="antismash"
                     /translation="EILLAGIWCKILGMARVGTADNFFSLGGDSIKSIQIISRVRAAGY
                     ELSMKDIFANQTIAGLARCLK"
     aSDomain        2602..3474
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B5D63_RS34445_Epimerization.1"
                     /evalue="4.70E-123"
                     /label="B5D63_RS34445_Epimerization.1"
                     /locus_tag="B5D63_RS34445"
                     /protein_end="1157"
                     /protein_start="866"
                     /score="403.4"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVTDG"
                     /tool="antismash"
                     /translation="GEAALTPVQRFFFDGPAAGDAHFNQSVMLHFPQGITAGEVQQIFG
                     YLQQHHDALRIVIKETAEGLRLDTKPEQPVSLTVYDAYNEAACTALQGSMDLTAGPLMK
                     LGLFREKDGSHLLIVVHHLVTDGVSWRILLEDMATLYGQLQQKQTLSLPGKTTPFLSWS
                     AHLHHYMESASFRSGRAYWQAQEKRGLQGFIPRDMPEGSNRGEEMRTVRFTLDSMRTHE
                     LLRQAPDTFRTSVEEVLLAAFVAAVAQQYGQQQVCVDLEGHGREEILPGVDISRTVGWF
                     TSIYPVMLEL"
     CDS_motif       2740..2781
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34445_0006"
                     /evalue="3.70E-03"
                     /label="Cy2"
                     /locus_tag="B5D63_RS34445"
                     /protein_end="926"
                     /protein_start="912"
                     /score="10.0"
                     /tool="antismash"
                     /translation="LQQHHDALRIVIKE"
     CDS_motif       2947..3012
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34445_0007"
                     /evalue="3.80E-11"
                     /label="NRPS-E2"
                     /locus_tag="B5D63_RS34445"
                     /protein_end="1003"
                     /protein_start="981"
                     /score="35.1"
                     /tool="antismash"
                     /translation="LLIVVHHLVTDGVSWRILLEDM"
     CDS_motif       3418..3468
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34445_0008"
                     /evalue="2.40E-09"
                     /label="NRPS-E5"
                     /locus_tag="B5D63_RS34445"
                     /protein_end="1155"
                     /protein_start="1138"
                     /score="29.1"
                     /tool="antismash"
                     /translation="DISRTVGWFTSIYPVML"
     CDS_motif       3508..3570
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34445_0009"
                     /evalue="3.30E-08"
                     /label="NRPS-E6"
                     /locus_tag="B5D63_RS34445"
                     /protein_end="1189"
                     /protein_start="1168"
                     /score="25.8"
                     /tool="antismash"
                     /translation="VKETLRTIPNKGIDYLLCRYY"
ORIGIN
        1 gcgggccatg caggagcagc accggtcata ctggaagagc gtttttgcgg atggcgtgcc
       61 cttgctgcac ttacccctgg attataaacg tccgttgata aaggacgata aaggtggtgt
      121 tgtccgtttt tccctgagca gggaagaggt atcagcttta caggcgctgg cgtcccggtc
      181 gcagtccacc ctttttatgg tgatgttgtc attgtacaac gtgctgctgt cacgcgtttg
      241 caaccaggat gatattgttg tgggcacatc ggtgtccggt cgttatcatg ctgatctgga
      301 accactgacc ggcatgtttg tcaatacgct ccctttacgc catcatccgc atggggacca
      361 gtcttttctg tcgtttttaa cgacggtgcg ggaccgtacg ctggagagtt ttgagcatca
      421 gtcttacccg ctggaggcaa tggtggaaga tctttcgccg gagcgtgaca cgagccgcag
      481 cccgttgttt gacgtgatgt ttggctatga gaacatagat gtgacgacgc tggccatacc
      541 gggattgaag ctggagcctt acctgcatga agagtatgct tccaaatttg atctgacact
      601 gaccgtcagt gagcggggcg atgatctgca gctggatttt gaatacgccg cctccttatt
      661 caacagcagc acggtccacc ggttgatcac ctattttaaa aatattgtac gggcagttat
      721 cgcagacaat gaagtgttat tgtcagctat cgttatgctt cccgaagcgg aggagcacac
      781 gctgttgcac gtgtttaaca atacagcgcg ttcatacggg aacaccactt ttccggatgt
      841 ttttaaagac caggcagcac tttatccgca tcatattgca gcgacatacc gtggcgttga
      901 cacagattac cgcacactgc atgaaacatc caacaggatg gcgcattacc tgcattcaat
      961 aggactgcct gcgggagctg tcgttccgtt gcttttggac cgtggcgtcg atctgttgtc
     1021 atggattatt ggtattcaga aagcaggcgg ggtatttatt gccctggata cccgtcatcc
     1081 cgcagaccgt attgccggta tactggatgg ctgtaatccg gcactggtgg taacggaaga
     1141 aaaatataca ggtctgttaa aggccggttg tccgatggta ttgcacgaaa ctgtggctac
     1201 gacagcacat gaccggtctg tcagcgattg gatgagcagg aaggcggcgg acattgcgta
     1261 tgccgcgtat acgtccgggt ctaccggtgt gccgaaaggt gtgttgttac accatggggg
     1321 aatgatgaac cactttcacg ggttgaagga attgctgggg ctaacatcgg atgacaggtt
     1381 tgcacaaacg gcagaatgca gctttgatgt ttatcttgtt cagatgctgc tggcgttgac
     1441 ggtgggtggt tgtacgcata tcatcgacag ggacgtgatg ctggatgccg gtcttctttc
     1501 agcggagctg gtggccggcg atattactat cctggaggtt gttccgtctg tggtccgttt
     1561 gctggtaaat gcggctcctt tgccattagg aaagcttcgc tgggttatct ccacagggga
     1621 ggcgctgagc agggagttgg cggttgcctg gtacagatgt tacccggatg tgcggttgct
     1681 gaacctctac gggccggctg aaacctcaga cgatgctact tattatattg ttccaccaga
     1741 tttacaggag cagggagtgg ggaacgtgcc tattggccag cctttgccca attttcacat
     1801 acatgtgctg gatgctgccc ggaacctgtg cccggtaggg gtaagcgggg agatctgtat
     1861 tgctggtccg ggtgtgggaa aaggttatat cgcagatgtt tcgctgacgg cgtcgaagtt
     1921 tgtaccgaac cctttcgcca gccatgatac atcggatgct tatgctgtaa tgtaccggac
     1981 aggtgacgta gggtaccggg acgaacaagg catgatctat ctcaccggcc ggatagataa
     2041 tatgataaag atccgtggcg cgcggataga gacaggcgac atcgaggctt tgctgttgca
     2101 gttcgccggt atcagggatg tattggtgtt aagccgtgaa aacaaagaac agcaatacct
     2161 ggcagcctat tatatcgcgg agggggaaat agacgcggcc gtcctggcgg cctatttaac
     2221 cggtaagctg ccggagtata tggtgccaac tgcatacgtg catttaaccg catttccgct
     2281 tacgctcagc ggcaaagtaa ataaacgcga actgccggaa cctgtatttg gcagcgaagc
     2341 attccaggcg cctgttaccg atgaggaaat acttctcgcc gggatatggt gcaagatact
     2401 gggcatggcg cgtgtgggaa cagctgataa ctttttctcc ctgggcggtg attccattaa
     2461 atccatccag attatttcac gcgtacgtgc ggccggatac gagttatcca tgaaagatat
     2521 ttttgccaat cagaccattg ccggattggc gaggtgcctg aaagtgctgc gggcacaacc
     2581 ccggcaggat atcgtaaaag gcgaggcggc actgacgccg gtacaacgct ttttctttga
     2641 cgggccggca gcaggggatg cgcatttcaa ccagtccgtg atgctgcatt tcccccaggg
     2701 cattaccgcc ggcgaggtac aacagatttt tggttacctg caacagcatc atgatgcgct
     2761 gcgcatcgtg ataaaagaaa ccgccgaagg cttgcggctg gacactaagc cggagcagcc
     2821 tgtatcgctg acggtatatg atgcatataa cgaggccgcc tgtacggctt tgcagggaag
     2881 catggacctc actgcgggcc cgttgatgaa actggggctg ttccgggaaa aagatggcag
     2941 ccacctgctg atcgtggtcc atcatctcgt gaccgatggc gtttcctggc gtatcctgct
     3001 ggaagacatg gccacgttgt acggccagtt acaacaaaaa caaacattat cgctgcccgg
     3061 caaaaccact ccttttcttt catggtccgc ccacctgcac cactacatgg agagcgcctc
     3121 attccgcagc gggcgcgcct actggcaggc gcaggaaaaa agagggcttc agggcttcat
     3181 tccccgcgac atgccggagg gcagcaaccg cggagaggaa atgcggaccg tacgtttcac
     3241 gctggacagt atgcgtacgc acgagttact gcgacaggcg ccggacactt tccgtacatc
     3301 ggtggaagag gtgttgctgg cggcttttgt tgccgctgtg gcgcagcaat acggacagca
     3361 acaggtgtgt gtggacctgg aaggccatgg ccgggaagaa atattgcccg gcgtggacat
     3421 aagccggaca gttggctggt ttacgagcat ttacccggtg atgctggagc taaaaggaac
     3481 aacgtggagc gaccggctgc gtcatgtaaa agagacgctg cgtaccattc ccaataaagg
     3541 gatcgactac ctgctctgcc ggtattacga taacagcctg gccgatgctg aagc
//
