LOCUS       NZ_FUWZ01000019         9323 bp    DNA     linear   CON 22-OCT-2024
DEFINITION  Chitinophaga eiseniae strain DSM 22224, whole genome shotgun
            sequence.
ACCESSION   NZ_FUWZ01000019
VERSION     NZ_FUWZ01000019.1
KEYWORDS    WGS; RefSeq.
SOURCE      Chitinophaga eiseniae
  ORGANISM  Chitinophaga eiseniae
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Chitinophaga.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2017) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900167135.1-RS_2024_10_22
            Annotation Date                   :: 10/22/2024 16:06:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,843
            CDSs (total)                      :: 6,771
            Genes (coding)                    :: 6,741
            CDSs (with protein)               :: 6,741
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:38:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9323
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FUWZ01000019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..2498)
                     /locus_tag="B5D63_RS34410"
                     /old_locus_tag="SAMN04488128_1191"
     CDS             complement(<1..2498)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (263-557). E-value:
                     1e-53. Score: 174.2. Matches aSDomain:
                     nrpspksdomains_B5D63_RS34410_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="B5D63_RS34410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488128_1191"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_143313378.1"
                     /sec_met_domain="Condensation (E-value: 2.6e-41, bitscore:
                     129.7, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 4.7e-24, bitscore:
                     72.7, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMKQQSTLDLSALDINSNIAAKNYWRNRLSDFQFVNYFKRRELPV
                     AALTAGMDSCAITADQAVKQAFNELAGSAAAKHIVLMAAIGALMSKYTSSADIMVFTPT
                     YKNEDNRVGGDDIIPFRMHAAETLTFAELVAAVKDNLVRDLGYSNYPVERILGKAKTAL
                     PWSSCIGMMVDEIQSDAAFGELPVGLLFRFDTSEGGLSLNISYNGTLYLPAFVNTIASR
                     FFNLLGKLLANRRVPLKSVALLTPDEKEKLFGEIVHCAQHSPLAASYHQERMWFIDYFE
                     AGDLYEGGPVYHNIPLILDLEGTPDVPLLAQSVQTLLDRYAILRTVIENADDRITQREI
                     TVSGFQFRYAAIDSDQDVEAVTSEEINTPMKLDSLLVRGLLINKGEKRFRMVLVFHHAI
                     ADRYSVKLLARQLLDNYYAGGNAVPAVPVVPYHHFSTWQRERFEDLEFHLFPFWDQLLS
                     GGRLKALELPTDRPRAKIHIYKAAVADIAWPQRITEKLLEYQAATGQNVGLILMAAFKV
                     LLYRYARHEEIVIGASADNRNVETLKGMIGPVANLVVIRSFVREDQSFKNYLDTLSAIY
                     DSCMAHSEMPFDKLVADLAPEKDMARTALFDVLFQYEETTNDLPSTAALLVTEQETNLG
                     YGKYDLNLLLQLRDNRLAGKLVFNEEYLDSATVADFLHNFYELAYNLISSPDERLSSIP
                     LLSAEAASSLLLSLDHSQVGYPSEATVHRLFERQAALHPGRTALAFNGDTMSYAALHAA
                     SDRVAAYLRNEGVKPGVIVGLLLERGMEVVVGMLGILKAGGAYLPLDVEYPASRKSYMI
                     SDSGMPFLLTARNVTDAGDYG"
     source          1..9323
                     /db_xref="taxon:634771"
                     /mol_type="genomic DNA"
                     /note="contig: Ga0070509_119"
                     /organism="Chitinophaga eiseniae"
                     /strain="DSM 22224"
                     /type_material="type strain of Chitinophaga eiseniae"
     region          1..9323
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..9323
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..9323
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:5622](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..5622)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(99..140)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34410_0005"
                     /evalue="7.70E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="B5D63_RS34410"
                     /protein_end="800"
                     /protein_start="786"
                     /score="19.0"
                     /tool="antismash"
                     /translation="LGILKAGGAYLPLD"
     CDS_motif       complement(663..761)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34410_0004"
                     /evalue="1.10E-11"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="B5D63_RS34410"
                     /protein_end="612"
                     /protein_start="579"
                     /score="37.1"
                     /tool="antismash"
                     /translation="MAHSEMPFDKLVADLAPEKDMARTALFDVLFQY"
     aSDomain        complement(828..1709)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B5D63_RS34410_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.00E-53"
                     /label="B5D63_RS34410_Condensation_LCL.1"
                     /locus_tag="B5D63_RS34410"
                     /protein_end="557"
                     /protein_start="263"
                     /score="174.2"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHAIADR"
                     /tool="antismash"
                     /translation="PLAASYHQERMWFIDYFEAGDLYEGGPVYHNIPLILDLEGTPDVP
                     LLAQSVQTLLDRYAILRTVIENADDRITQREITVSGFQFRYAAIDSDQDVEAVTSEEIN
                     TPMKLDSLLVRGLLINKGEKRFRMVLVFHHAIADRYSVKLLARQLLDNYYAGGNAVPAV
                     PVVPYHHFSTWQRERFEDLEFHLFPFWDQLLSGGRLKALELPTDRPRAKIHIYKAAVAD
                     IAWPQRITEKLLEYQAATGQNVGLILMAAFKVLLYRYARHEEIVIGASADNRNVETLKG
                     MIGPVANLVVIRS"
     CDS_motif       complement(831..920)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34410_0003"
                     /evalue="9.80E-05"
                     /label="C5_LCL_267-296"
                     /locus_tag="B5D63_RS34410"
                     /protein_end="556"
                     /protein_start="526"
                     /score="14.9"
                     /tool="antismash"
                     /translation="EIVIGASADNRNVETLKGMIGPVANLVVIR"
     CDS_motif       complement(1251..1316)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34410_0002"
                     /evalue="3.80E-04"
                     /label="C3_DCL_135-156"
                     /locus_tag="B5D63_RS34410"
                     /protein_end="416"
                     /protein_start="394"
                     /score="13.2"
                     /tool="antismash"
                     /translation="FHHAIADRYSVKLLARQLLDNY"
     CDS_motif       complement(1515..1622)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34410_0001"
                     /evalue="4.20E-11"
                     /label="C2_LCL_024-062"
                     /locus_tag="B5D63_RS34410"
                     /protein_end="328"
                     /protein_start="292"
                     /score="35.3"
                     /tool="antismash"
                     /translation="HNIPLILDLEGTPDVPLLAQSVQTLLDRYAILRTVI"
     gene            complement(2509..5622)
                     /locus_tag="B5D63_RS34415"
                     /old_locus_tag="SAMN04488128_1192"
     CDS             complement(2509..5622)
                     /NRPS_PKS="Domain: AMP-binding (10-407). E-value: 4.5e-79.
                     Score: 257.8. Matches aSDomain:
                     nrpspksdomains_B5D63_RS34415_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (515-583). E-value: 1.7e-19. Score:
                     61.8. Matches aSDomain: nrpspksdomains_B5D63_RS34415_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (599-886). E-value:
                     2.3e-73. Score: 238.8. Matches aSDomain:
                     nrpspksdomains_B5D63_RS34415_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="B5D63_RS34415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488128_1192"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_078673430.1"
                     /sec_met_domain="AMP-binding (E-value: 2.7e-91, bitscore:
                     294.2, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.6e-16, bitscore:
                     48.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 3.4e-61, bitscore:
                     195.0, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVFQEELNRCLKQFAGETAIHCNNISISYAQLLADANRITAFLLE
                     RNPGKGTMVGVYLEDRASLITTAIGVLNAGCVFVPVDCNLPQQRLDSMVEKMGLQYFIT
                     SGLNTKIKPDHTFLFGDILAEKKGPVDVCYPVFDESDNIYVYFTSGSTGTPKGILGRNE
                     SLLQFIQWEIAEFDIRPGYRVSQFISPYFDAFLRDIFVPLLAGGTICIPPAEQTFHEAA
                     HITTWINENNINLVHCVPSLFRLINHDALQAADYGSLQHVLLSGEKINPAELKPWYDTF
                     GARIGLVNLYGPSETTMIRTFYRIQPADAAQPRMPIGRPIPGTKILIAKKDLTPCEKLV
                     PGELYIISAYSSNGYLNEPELNKERFITIHAGTKEEAKAFKTGDIARMLVNGNIDLIGR
                     EDRQVKLRGARIELDEIEHVLQRVDSLRQAVVMKHQDEQNNELLIAFLVRRQDIADDID
                     IRQLSRSYLEEHLPSYMLPADFREVKAIPLLSNGKINYRELLAQAPQHTVVAPENETER
                     RLLEVWKQTLGEKEISTEDSFYKVGGNSLNTMKLIGRIHKEFNVRISLSELFNHLTIKK
                     QAGIISKLHSDNLMTIGKAPEMPDYPLSVAQERIYYNYQLNKTGMAFNLPVAWTLNSAF
                     DATKMAAALTALVQRHESLRTDFGFVDDQLVQRIADHVDVEIAVIHTGEASLRAEVQAF
                     IRPFDLSKPGLLRCGIIRTDKGTAVLVVDAHHIVCDGITQVILFNDLIRIYNGETPEPL
                     PVQYKDYALWESQFRKTELYMSQREFWLNQFEGAIPSTNFPVTGVQNDLADDAGGNIFF
                     SIEKASALLFARMLKEEEITVSSGLFTVFFVFLALLTGQEDVIAGISTSGRLQHELEGV
                     AGMFVKTLPVRYQLDTDLTFRSFTKAMHAHLVEVISRQQFDLSDIFSELNASRGANVSE
                     LFKAVFVYQDFDVNTTDADHLLTPFELSNAAAKYPVTLFASGREDAYTFRFEYQSSYFL
                     AKDAALLVDRLTALFNTIAENPDISLSSLAGYNTVPDNVMKDHIILNL"
     aSDomain        complement(2965..3825)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B5D63_RS34415_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="2.30E-73"
                     /label="B5D63_RS34415_Condensation_LCL.1"
                     /locus_tag="B5D63_RS34415"
                     /protein_end="886"
                     /protein_start="599"
                     /score="238.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIVCDG"
                     /tool="antismash"
                     /translation="DYPLSVAQERIYYNYQLNKTGMAFNLPVAWTLNSAFDATKMAAAL
                     TALVQRHESLRTDFGFVDDQLVQRIADHVDVEIAVIHTGEASLRAEVQAFIRPFDLSKP
                     GLLRCGIIRTDKGTAVLVVDAHHIVCDGITQVILFNDLIRIYNGETPEPLPVQYKDYAL
                     WESQFRKTELYMSQREFWLNQFEGAIPSTNFPVTGVQNDLADDAGGNIFFSIEKASALL
                     FARMLKEEEITVSSGLFTVFFVFLALLTGQEDVIAGISTSGRLQHELEGVAGMFVKTLP
                     VRYQLD"
     CDS_motif       complement(2977..3066)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34415_0007"
                     /evalue="1.20E-07"
                     /label="C5_LCL_267-296"
                     /locus_tag="B5D63_RS34415"
                     /protein_end="882"
                     /protein_start="852"
                     /score="24.2"
                     /tool="antismash"
                     /translation="DVIAGISTSGRLQHELEGVAGMFVKTLPVR"
     CDS_motif       complement(3325..3357)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34415_0006"
                     /evalue="9.00E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="B5D63_RS34415"
                     /protein_end="766"
                     /protein_start="755"
                     /score="12.0"
                     /tool="antismash"
                     /translation="QYKDYALWESQ"
     CDS_motif       complement(3388..3450)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34415_0005"
                     /evalue="2.10E-06"
                     /label="C3_LCL_132-143"
                     /locus_tag="B5D63_RS34415"
                     /protein_end="745"
                     /protein_start="724"
                     /score="20.3"
                     /tool="antismash"
                     /translation="HHIVCDGITQVILFNDLIRIY"
     CDS_motif       complement(3655..3759)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34415_0004"
                     /evalue="3.30E-11"
                     /label="C2_LCL_024-062"
                     /locus_tag="B5D63_RS34415"
                     /protein_end="656"
                     /protein_start="621"
                     /score="35.6"
                     /tool="antismash"
                     /translation="AFNLPVAWTLNSAFDATKMAAALTALVQRHESLRT"
     aSDomain        complement(3874..4077)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B5D63_RS34415_PCP.1"
                     /evalue="1.70E-19"
                     /label="B5D63_RS34415_PCP.1"
                     /locus_tag="B5D63_RS34415"
                     /protein_end="583"
                     /protein_start="515"
                     /score="61.8"
                     /tool="antismash"
                     /translation="ERRLLEVWKQTLGEKEISTEDSFYKVGGNSLNTMKLIGRIHKEFN
                     VRISLSELFNHLTIKKQAGIISK"
     aSModule        complement(3874..5592)
                     /complete
                     /domains="nrpspksdomains_B5D63_RS34415_AMP-binding.1"
                     /domains="nrpspksdomains_B5D63_RS34415_PCP.1"
                     /locus_tags="B5D63_RS34415"
                     /monomer_pairings="X -> X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(4372..4434)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34415_0003"
                     /evalue="4.40E-09"
                     /label="NRPS-A_a8"
                     /locus_tag="B5D63_RS34415"
                     /protein_end="417"
                     /protein_start="396"
                     /score="28.6"
                     /tool="antismash"
                     /translation="IGREDRQVKLRGARIELDEIE"
     aSDomain        complement(4402..5592)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B5D63_RS34415_AMP-binding.1"
                     /evalue="4.50E-79"
                     /label="B5D63_RS34415_AMP-binding.1"
                     /locus_tag="B5D63_RS34415"
                     /protein_end="407"
                     /protein_start="10"
                     /score="257.8"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="LKQFAGETAIHCNNISISYAQLLADANRITAFLLERNPGKGTMVG
                     VYLEDRASLITTAIGVLNAGCVFVPVDCNLPQQRLDSMVEKMGLQYFITSGLNTKIKPD
                     HTFLFGDILAEKKGPVDVCYPVFDESDNIYVYFTSGSTGTPKGILGRNESLLQFIQWEI
                     AEFDIRPGYRVSQFISPYFDAFLRDIFVPLLAGGTICIPPAEQTFHEAAHITTWINENN
                     INLVHCVPSLFRLINHDALQAADYGSLQHVLLSGEKINPAELKPWYDTFGARIGLVNLY
                     GPSETTMIRTFYRIQPADAAQPRMPIGRPIPGTKILIAKKDLTPCEKLVPGELYIISAY
                     SSNGYLNEPELNKERFITIHAGTKEEAKAFKTGDIARMLVNGNIDLIGREDRQVKLR"
     CDS_motif       complement(4525..4605)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34415_0002"
                     /evalue="4.30E-07"
                     /label="NRPS-A_a6"
                     /locus_tag="B5D63_RS34415"
                     /protein_end="366"
                     /protein_start="339"
                     /score="22.3"
                     /tool="antismash"
                     /translation="VPGELYIISAYSSNGYLNEPELNKERF"
     CDS_motif       complement(5146..5193)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34415_0001"
                     /evalue="8.90E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="B5D63_RS34415"
                     /protein_end="159"
                     /protein_start="143"
                     /score="21.4"
                     /tool="antismash"
                     /translation="YVYFTSGSTGTPKGIL"
     gene            complement(5638..7497)
                     /gene="asnB"
                     /locus_tag="B5D63_RS34420"
                     /old_locus_tag="SAMN04488128_1193"
     CDS             complement(5638..7497)
                     /EC_number="6.3.5.4"
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="asnB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /locus_tag="B5D63_RS34420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488128_1193"
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="WP_078673431.1"
                     /sec_met_domain="GATase_7 (E-value: 7.9e-46, bitscore:
                     143.2, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 6.2e-71, bitscore:
                     227.9, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCGICGYIRYGAHTTGYDEQVVRDMNRQLLHRGPDSQCVHQFDNV
                     ALGFSRLSIIGLDNGMQPVLNEDESVVLLCNGEIFNYIELREMLRAKGHRFRTASDVEV
                     IVHLYEEHGMAFLNELNGQFAFALYDKKKKTLYCARDHMGILPFFYTIRQDTFIFGSEI
                     KAILKHPLVEPRVDLTGLDQVFTFPGLISPRTMFKDIFSLENGHFLSVDHDGLVTKSQY
                     WDLVFPDKDEVVEEMPAEYYINRLETLFENAVRLRLRADVPYGLYLSGGLDSSMIAMKV
                     HALDPSMAKHVFSIDFVDSRLSETLYQQLVAKEIGAKINRKTFFFDDISERLQQAVYHS
                     ECPVKESYNTASLSLSAAVRNNNIKVILSGEGADELFAGYVGYRFDKIRAMTGQQQVVS
                     ATEAAWRERVWGDPAFFYENNFETLCEQKKNLYSADVAAVFDDINCLNHFVIDKSRIAN
                     RDVLNQRAYIDYKLRLVDHLVSDHGDRMAMANSVEVRYPFLDKDFVTFAATVPPSLKLN
                     ELEEKYVLKKMGSRFTPEKIVEREKFGFTAPGSPYLMKRNIEYIHDTLSSARINQQGIF
                     NPVMIEELKSLYTTEGFTINTPFENDVLMTVLTFGILLEQYFN"
     CDS             complement(7517..>9321)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PP-binding (95-161). E-value: 5.7e-19.
                     Score: 60.2. Matches aSDomain:
                     nrpspksdomains_B5D63_RS34425_PP-binding.1"
                     /NRPS_PKS="Domain: Condensation_LCL (178-458). E-value:
                     1.7e-62. Score: 203.1. Matches aSDomain:
                     nrpspksdomains_B5D63_RS34425_Condensation_LCL.1"
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="B5D63_RS34425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488128_1194"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_143313380.1"
                     /sec_met_domain="PP-binding (E-value: 7.4e-19, bitscore:
                     56.3, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 5.4e-59, bitscore:
                     187.7, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GEIVYHVCRQPGVREAAVLQRNGQLACYYVSDEAQDSAALQGALQ
                     QVLPAYMVPVYYLHLHHLPLTGNGKLDESRLPAPEAGVGGYAAPTGDTAIRLAAIWADV
                     LKLDVSDISATASFFGMGGHSVKAMRLKNLVHKHFGCNIELRDIFTHAVLNDMAAFIGS
                     SQPAAGIELLKAGAQQECAASPAQERLYYQQMLNKDNLGFNIAAVFRLADTVVPEKIHA
                     AFIQLQQRHTSLRTRFELGEGGLVQKVDTEVAPALQDAGPENAPFEAVWQQFIRPFDLS
                     VAPLMRYALWKDFLLIDIHHVICDGISLNILIRDLRDLYQGKVLTPVEYNYLDYAASLA
                     TRTADNDKNKRYWLRQLSGELPRLDLPVMQQRNFPGIHPAAVKTLEIQEEQFFQLQQCA
                     ATAATSPYVLLLSAFYILLYKVCGNKTLIAGIDAAGRSHEALQEVVGTFVNILPLKVDI
                     HEDQPVHTLLAGVKNCLLDALDHQDYQFDQIVALAEEEERDGRNPVFDVHFAFANTLDS
                     DAELKELGFIPVQGRQHETTQYELKIEVSEKEAGYAVAFIYSTVLYEESMMDVFVAYYQ
                     RIVNAIAQDSEALIDDIRLETTVNV"
     gene            complement(7517..>9323)
                     /locus_tag="B5D63_RS34425"
                     /old_locus_tag="SAMN04488128_1194"
     CDS_motif       complement(7787..7903)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34425_0004"
                     /evalue="7.60E-08"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="B5D63_RS34425"
                     /protein_end="511"
                     /protein_start="472"
                     /score="24.6"
                     /tool="antismash"
                     /translation="NCLLDALDHQDYQFDQIVALAEEEERDGRNPVFDVHFAF"
     aSDomain        complement(7946..8785)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B5D63_RS34425_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.70E-62"
                     /label="B5D63_RS34425_Condensation_LCL.1"
                     /locus_tag="B5D63_RS34425"
                     /protein_end="458"
                     /protein_start="178"
                     /score="203.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHVICDG"
                     /tool="antismash"
                     /translation="QECAASPAQERLYYQQMLNKDNLGFNIAAVFRLADTVVPEKIHAA
                     FIQLQQRHTSLRTRFELGEGGLVQKVDTEVAPALQDAGPENAPFEAVWQQFIRPFDLSV
                     APLMRYALWKDFLLIDIHHVICDGISLNILIRDLRDLYQGKVLTPVEYNYLDYAASLAT
                     RTADNDKNKRYWLRQLSGELPRLDLPVMQQRNFPGIHPAAVKTLEIQEEQFFQLQQCAA
                     TAATSPYVLLLSAFYILLYKVCGNKTLIAGIDAAGRSHEALQEVVGTFVNILPLKVDI"
     CDS_motif       complement(7958..8026)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34425_0003"
                     /evalue="9.50E-07"
                     /label="C5_LCL_267-296"
                     /locus_tag="B5D63_RS34425"
                     /protein_end="454"
                     /protein_start="431"
                     /score="21.3"
                     /tool="antismash"
                     /translation="DAAGRSHEALQEVVGTFVNILPL"
     CDS_motif       complement(8360..8425)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34425_0002"
                     /evalue="6.80E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="B5D63_RS34425"
                     /protein_end="320"
                     /protein_start="298"
                     /score="28.2"
                     /tool="antismash"
                     /translation="IHHVICDGISLNILIRDLRDLY"
     CDS_motif       complement(8606..8713)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B5D63_RS34425_0001"
                     /evalue="2.20E-07"
                     /label="C2_LCL_024-062"
                     /locus_tag="B5D63_RS34425"
                     /protein_end="238"
                     /protein_start="202"
                     /score="23.3"
                     /tool="antismash"
                     /translation="FNIAAVFRLADTVVPEKIHAAFIQLQQRHTSLRTRF"
     aSDomain        complement(8837..9034)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B5D63_RS34425_PP-binding.1"
                     /evalue="5.70E-19"
                     /label="B5D63_RS34425_PP-binding.1"
                     /locus_tag="B5D63_RS34425"
                     /protein_end="161"
                     /protein_start="95"
                     /score="60.2"
                     /tool="antismash"
                     /translation="RLAAIWADVLKLDVSDISATASFFGMGGHSVKAMRLKNLVHKHFG
                     CNIELRDIFTHAVLNDMAAFI"
ORIGIN
        1 ccgtaatcgc cggcatcggt cacgttgcgc gccgtcagca aaaacggcat accactgtcg
       61 ctgatcatat acgatttacg ggaagcaggg tattccacgt ccaacggcag ataagcgccg
      121 ccggccttca agatccccag catcccgacc accacctcca tgccccgctc cagcagcaac
      181 cctacgatca cgcccggctt aacgccctcg ttgcgcagat acgccgcaac ccggtcgctg
      241 gcagcgtgca gcgccgcata actcatggtg tcgccattga acgccagggc agtacggccg
      301 ggatgcagcg cggcctgccg ctcaaacagc cggtgtaccg ttgcctccga aggatatcct
      361 acctggctgt ggtccagcga cagcagcaac gacgatgctg cttccgcgga caacagcggg
      421 atagaagaga ggcgctcatc cggcgaggag atgaggttat aggccaattc gtaaaagtta
      481 tgcaggaaat ccgctaccgt cgcgctatcc aggtactctt cattaaatac cagctttcct
      541 gctaacctgt tgtcccgaag ctgcaataac aagttcaggt catattttcc gtatcccaga
      601 tttgtttcct gttcggttac cagcagcgct gccgttgatg gcagatcatt ggttgtttct
      661 tcatattgga aaagcacgtc gaacagcgcg gtcctggcca tatctttttc cggcgccaga
      721 tcggccacca gcttgtcaaa aggcatctcg ctgtgtgcca tacagctgtc ataaatggcc
      781 gacaaagtat ccaggtagtt tttaaaagac tggtcttccc gtacaaaact cctgatcacc
      841 accaggttgg cgaccggacc gatcattcct ttgagcgttt caacattcct gttatcagcg
      901 ctggcgccaa tgacaatttc ttcatgccgt gcatacctgt acaacaatac cttaaaggcg
      961 gccattaata tcaacccgac gttctggccg gtcgctgcct ggtattccag cagtttttca
     1021 gtaatccgct ggggccatgc gatatcagcc accgcggcct tatagatatg tatttttgct
     1081 cttggcctgt ctgttggcag ttcaagcgcc tttaacctgc caccggacaa cagctggtcc
     1141 cagaaaggga ataaatgaaa ttccaggtcc tcaaaacgtt cccgctgcca tgttgaaaaa
     1201 tgatgataag gtactaccgg tactgccggc accgcattgc caccggcgta gtagttatca
     1261 agaagttgac gggccagcag tttaacggaa tagcgatctg ctatggcatg atggaatacc
     1321 aggaccatcc ggaaccgttt ctctccttta ttgatcagca agcctctcac cagcaaactg
     1381 tccagcttca ttggcgtatt gatttcttcg ctggtaactg cttcaacgtc ctggtcactg
     1441 tcaatggccg catagcggaa ctgaaacccg gaaaccgtga tctcccgctg cgtgatacgg
     1501 tcgtctgcat tctctataac cgttcttaat atcgcataac ggtcaagtaa cgtttgtacg
     1561 ctctgcgcta acagcgggac atccggcgtt ccttccagat caagtatcaa tgggatatta
     1621 tggtaaaccg ggccgccttc atacaggtca cctgcttcaa aataatcgat gaaccacatg
     1681 cgttcctggt gataagaggc tgccaggggg ctatgctgag cgcaatggac gatctccccg
     1741 aacagctttt ccttctcatc aggtgtcagc agcgccacgc ttttcagggg gacccttctg
     1801 tttgccagta gtttaccaag caggttaaag aaccggctgg caatggtatt tacaaaagca
     1861 ggaagatata aagtaccgtt atagctgata ttcaggctta atcccccttc gctggtatca
     1921 aaacgaaaaa gcagccccac cggtagctcc ccgaaagcag catcagactg tatttcgtcc
     1981 accatcatcc cgatgcagga tgaccagggc aatgccgtct tcgcttttcc caatatgcgt
     2041 tccacagggt agttgctata tccgaggtct ctcaccaggt tatctttcac ggcggccacc
     2101 agctccgcaa atgtcagggt ttctgccgcg tgcatacgaa aaggaatgat gtcatctcct
     2161 ccgaccctgt tatcttcatt tttataggtg ggggtaaaca ccatgatatc ggccgaactg
     2221 gtgtacttac tcatcagcgc cccgatagcg gccatcagca cgatatgttt cgccgccgca
     2281 gatccggcca gttcattgaa agcctgcttt acagcctggt cagcggtaat ggcgcaacta
     2341 tccatgccag cagtcagcgc tgctaccggc aactcccgcc ttttaaaata atttacaaac
     2401 tgaaaatccg acaatcggtt tctccagtaa ttcttcgcgg cgatattact gtttatgtca
     2461 agtgcggaca agtccagggt cgattgctgt ttcatcatat aaggctaact agaggttgag
     2521 aatgatatga tccttcatca cgttatcggg cacggtatta tagccggcca gagaagacag
     2581 gctgatatcg ggattttctg caatagtgtt aaacaacgcc gtcagcctgt ctaccagcag
     2641 cgctgcgtct tttgccagga aataagatga ctggtattca aaacggaacg tataggcatc
     2701 ttcacgtccg gaagcaaaca gtgtgacagg atatttggcg gcggcattgc ttaattcgaa
     2761 gggcgtcagc aaatggtccg cgtcagtggt gttgacatca aaatcctggt aaacaaacac
     2821 ggctttgaac aattcgctca cattagcgcc cctggaagca ttcagctcac tgaaaatatc
     2881 ggacaggtcg aattgctggc ggctgatcac ctctacaaga tgggcatgca tggcttttgt
     2941 aaaagaccgg aaagtgagat ctgtgtccag ctgatacctg accggcaatg tctttacaaa
     3001 catgcccgcc acaccttcca gttcatgctg caaacggccg gaggtgctaa tccccgcaat
     3061 aacgtcttcc tgtcctgtca gcagcgccag gaagacaaag aacaccgtaa acaggccgga
     3121 cgaaactgtg atctcctctt ctttcagcat acgggcaaat agtaatgccg acgctttttc
     3181 gatactgaaa aagatgttac cgcccgcatc gtctgccagg tcattttgca cacctgtcac
     3241 aggaaaattg gtcgatggaa tggcgccttc gaactggttc agccagaact cccgctggga
     3301 catgtacaat tccgttttcc tgaactggct ttcccacaag gcatagtctt tatattgcac
     3361 aggtaagggt tccggcgttt cgccattata gatccggatc agatcattaa acaggatcac
     3421 ctgcgtaatt ccgtcgcaaa caatatggtg cgcatctacc accagcacag cagtcccttt
     3481 gtccgtacgg ataatgccgc agcgcagcaa accaggctta ctcaggtcaa aaggccggat
     3541 aaaagcttgt acttccgctc tcagtgatgc ctccccggta tgaataacag ctatctcaac
     3601 gtcaacatga tccgcgattc gctgcaccag ctgatcatcc acaaaaccaa aatcggtacg
     3661 gagactttca tggcgttgca ccagtgctgt gagcgcagcc gccattttcg ttgcgtcgaa
     3721 cgcgctgttc aacgtccagg ctacgggcag gttaaaagcc attcccgttt tattgagctg
     3781 atagttatag tagattcttt cctgtgcaac ggaaagggga taatcgggca tttccggggc
     3841 tttgccaatg gtcatcaggt tatcgctatg cagtttgctg atgatcccgg cctgtttttt
     3901 aattgtgaga tgattaaaca gctcgctgag agaaatcctg acattgaatt ccttatgaat
     3961 tctgccgatg agcttcatgg tgttcagcga gttgccccct actttataaa agctgtcctc
     4021 cgttgatatc tccttttcgc ccagcgtttg tttccatacc tccagcagcc tgcgctctgt
     4081 ttcattttcc ggcgcaacga cagtatgctg cggcgcctgg gccaatagtt ccctgtagtt
     4141 gatcttcccg ttagacaaca acggaattgc cttcacttcc cgaaaatcag cggggagcat
     4201 gtatgacggc aaatgttctt cgaggtagct gcggctcagt tgcctgatat ctatatcatc
     4261 tgcgatgtcc tgcctgcgta ccaggaaagc gatcagcaat tcattgtttt gttcgtcctg
     4321 gtgcttcatc acaacggcct ggcgcaggct atccactctt tgcaggacgt gttcaatttc
     4381 atccagttct attctggccc cccgtaattt cacctgcctg tcttccctgc cgatgagatc
     4441 tatgttaccg ttgaccagca tacgggcgat gtccccggtt ttaaaggctt ttgcttcttc
     4501 ttttgttccg gcatgaatgg taataaagcg ctctttgttc agctccggtt cattcaggta
     4561 accgtttgaa ctataggcag agataatgta caattcgccg ggcaccagct tttcacaggg
     4621 tgtaaggtct tttttggcaa tcaatatctt tgtgcccggt atgggacggc caatgggcat
     4681 cctgggttgt gcggcatcgg caggctgtat cctgtaaaaa gttctgatca tggtggtttc
     4741 ggaagggcca tacaaattca ccagccctat gcgcgcacca aaggtgtcgt accatggttt
     4801 cagctcggcc gggttgattt tctcaccgga gagcaatacg tgttgcaggc ttccataatc
     4861 agctgcctgt aacgcatcgt gattgatcag gcggaaaagg ctggggacac aatggaccag
     4921 gttgatattg ttttcgttta tccatgtcgt tatatgggcc gcttcatgga atgtctgttc
     4981 tgccggggga atacagatgg taccgccggc cagcaggggt acaaaaatat cccggagaaa
     5041 agcatcgaaa taagggctga tgaactggct aaccctgtat cccggccgga tatcgaattc
     5101 cgcgatttcc cattgtatga attgcagcag gctttcattc cttcccagaa tacctttcgg
     5161 cgtaccggta gaaccggacg taaaataaac atagatgttg tccgactcat caaagacagg
     5221 atagcagaca tcaaccggtc ctttcttttc cgctaatatg tccccgaaca ggaaggtatg
     5281 gtccggcttt atttttgtgt tcaggccgga agtaataaaa tattgcagcc ccatcttctc
     5341 caccatactg tcgagccgct gttgcggcag gttgcagtcc acaggaacaa atacgcatcc
     5401 ggcgttcaat acgccgatgg cagtggtgat aagcgaggcc ctgtcttcga ggtatacccc
     5461 caccatcgtg cctttaccgg gatttctttc caggagaaag gcggtgatac ggttggcatc
     5521 cgcaagcagc tgcgcataag agatggatat attattgcag tgaatagctg tttcgccggc
     5581 aaattgcttt aaacatctgt tcagttcttc ctgaaaaacc ataggtgcta tatttgatta
     5641 attgaagtat tgctccagca ggataccgaa ggtgagtacc gtcatgagca catcattttc
     5701 aaaaggggta ttaatggtaa atccttctgt cgtatagagc gatttcagtt cttctatcat
     5761 taccggattg aagatgccct gctggtttat tcttgctgaa gaaagggtat catggatgta
     5821 ttcaatattt ctcttcatca gataagggct gccgggtgcg gtaaatccaa acttttctct
     5881 ttccacaatt ttttcaggag taaagcggct tcccattttt ttgaggacat acttttcttc
     5941 caactcattc agcttcagtg aggggggcac cgtagcagca aatgtcacaa aatctttatc
     6001 caggaagggg taacggactt cgacggaatt agccatggcc atccggtcgc cgtggtccga
     6061 taccaggtgg tccaccagtc gcagcttata atcgatataa gccctttggt ttaagacatc
     6121 cctgttggca atacggcttt tatcgataac gaagtgattc aggcagttga tgtcatcgaa
     6181 taccgcagcg acatccgccg agtacaggtt tttcttctgt tcacacaacg tttcaaagtt
     6241 attttcgtaa aagaatgcag gatcgcccca tactctttcg cgccaggcgg cttcagttgc
     6301 agaaacaacc tgttgttgcc ctgtcatggc cctgatctta tcaaagcggt agcctacgta
     6361 cccggcaaac agttcgtcgg cgccttctcc ggagaggatc actttgatat tgttgtttct
     6421 caccgccgcc gacagggaca aagacgccgt attataggat tcttttaccg gacactccga
     6481 atggtaaaca gcctgttgta aacgttcact gatatcgtca aagaagaagg tcttcctgtt
     6541 gatcttagcg ccgatttctt ttgccaccaa ttgctggtag agggtttcag aaagacgtga
     6601 atcaacgaag tcaattgaaa acacatgttt ggccatcgaa gggtccagcg catgtacttt
     6661 catggcaatc atcgaggagt ccagccctcc actgagataa agcccatatg gcacgtctgc
     6721 gcgtagccgc aggcgcacgg cattttcaaa aagcgtctcc aggcggttga tataatattc
     6781 ggcgggcatc tcctctacaa cttcgtcctt gtccgggaaa acaaggtccc agtattggct
     6841 tttggtaaca agcccgtcgt ggtccacact taaaaaatga ccgttttcca ggctgaaaat
     6901 atctttgaac atcgttctgg gactaataag accgggaaac gtgaatacct ggtcaagtcc
     6961 cgtcaggtct acccggggtt ccacaagcgg gtgcttcagg atcgctttta tttcggaacc
     7021 gaatatgaaa gtatcctgcc ggatcgtgta gaagaatggc agaattccca tgtggtcacg
     7081 ggcacagtac aatgtttttt tctttttgtc gtagagcgca aaagcaaact gaccgttcaa
     7141 ttcattcaga aaggccatcc catgctcctc atagaggtga acgatcactt ccacgtctga
     7201 agccgtgcga aaccggtgcc cttttgcgcg aagcatttcc cgcagctcaa tatagttgaa
     7261 gatctcccca ttgcacaaca acaccacgct ttcgtcttca ttcaaaacag gctgcatgcc
     7321 gttgtctaaa ccgatgatgc tcaaccggct gaagcccagc gccacattat caaactggtg
     7381 cacgcactga ctgtcgggcc cccggtgcaa cagttgcctg ttcatgtccc ggaccacctg
     7441 ttcatcatac cctgttgtat gcgccccata ccttatgtag ccacaaatac cacacatatt
     7501 aaaattgctt tagtagttat acatttacag tcgtttcaag ccggatatca tcaatcaatg
     7561 cttcgctgtc ttgcgcgatg gcattgacga tgcgctgata gtacgccaca aacacgtcca
     7621 tcatgctctc ttcatacaac accgtgctgt agatgaatgc gactgcgtaa cctgcttctt
     7681 tttctgatac ttctattttt aattcgtatt gggtagtctc atgctggcgg ccctgtacag
     7741 gaataaatcc cagctctttc agttccgcat cgctgtccag cgtattggca aaagcaaaat
     7801 gaacatcaaa aaccggatta cggccatccc gctcttcttc ttccgccaat gctactatct
     7861 gatcgaactg atagtcctga tggtccagtg cgtccagtaa gcagtttttg acacctgcca
     7921 gcaatgtatg aacaggctgg tcttcatgga tatctacttt caacggcagg atattcacaa
     7981 aagtgccgac tacttcctgt agcgcctcat ggcttctgcc ggctgcatcg ataccggcga
     8041 taagggtttt gttgccacag accttgtaca acagtatata gaaagcggac aacaacaaca
     8101 catagggaga agtggctgcc gtggcggcac attgttgcag ttgaaagaac tgttcctcct
     8161 gtatttccag cgttttgacg gcagccgggt gtatgcccgg gaaattacgt tgttgcatca
     8221 ccggcaaatc cagccgggga agctctcccg acagctgccg cagccaatag cgtttgtttt
     8281 tgtcgttgtc tgccgtccta gtagccaggc tggcggcgta gtcgaggtaa ttatattcaa
     8341 cgggcgtcaa tactttcccc tggtagaggt cccgcaggtc tctgatgagt atgttcagag
     8401 aaataccatc acagatcaca tggtggatat caatcaacaa aaaatccttc cacaaggcat
     8461 agcgcatgag tggcgcaacg gaaagatcaa agggccggat gaactgctgc cacaccgctt
     8521 caaagggtgc attttcaggg cctgcgtctt gcagcgcagg cgcgacctcc gtatccactt
     8581 tttgtaccag tcctccttcg ccgagttcaa agcgggtccg caggctggtg tgccgctgtt
     8641 gcaattggat aaatgccgcg tgtatctttt ccggcacaac ggtgtccgcc aaccggaaga
     8701 ccgcggctat gttaaatcca aggttatcct tattcagcat ttgctgatag tacagtcgtt
     8761 cctgggcagg ggatgccgca cattcctgct gcgcgccggc tttgaggagt tctatccctg
     8821 cagcaggctg actgctcccg ataaaagcag ccatgtcatt caatacggca tgtgtaaaga
     8881 tatcgcgcag ctctatgtta cagccgaagt gtttgtgaac aagattcttc aatcgcatgg
     8941 ccttgacaga gtgcccgccc atcccaaaaa agctggcggt ggcgctgatg tccgacacgt
     9001 ccagtttcag tacatcggcc cagatggccg ccaaccggat agcagtatcg ccggtcggcg
     9061 ccgcatagcc cccgactccc gcttcgggag caggcagccg gctttcatcc agcttaccgt
     9121 tgcccgtcag gggcaggtga tggagatgga ggtaatacac aggcaccata taggcaggca
     9181 acacctgctg caacgcgcct tgcagcgctg cgctgtcctg tgcttcgtcg gatacgtagt
     9241 agcatgccag ctgaccgtta cgctgcaaca ccgcggcttc tctcacaccg ggctgacggc
     9301 aaacatggta cacgatctcg ccg
//
