LOCUS       NZ_FUWZ01000001        11143 bp    DNA     linear   CON 22-OCT-2024
DEFINITION  Chitinophaga eiseniae strain DSM 22224, whole genome shotgun
            sequence.
ACCESSION   NZ_FUWZ01000001
VERSION     NZ_FUWZ01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Chitinophaga eiseniae
  ORGANISM  Chitinophaga eiseniae
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Chitinophaga.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2017) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900167135.1-RS_2024_10_22
            Annotation Date                   :: 10/22/2024 16:06:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,843
            CDSs (total)                      :: 6,771
            Genes (coding)                    :: 6,741
            CDSs (with protein)               :: 6,741
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 1 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:38:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 218980
            Orig. end                         :: 230123
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FUWZ01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11143
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11143
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11143
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6143](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6143
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    1570..1582
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 19.6"
                     /tool="antismash"
     misc_feature    complement(1651..1666)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.35"
                     /tool="antismash"
     gene            2046..2780
                     /locus_tag="B5D63_RS00825"
                     /old_locus_tag="SAMN04488128_101164"
     CDS             2046..2780
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B5D63_RS00825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN04488128_101164"
                     /product="hypothetical protein"
                     /protein_id="WP_078666893.1"
                     /transl_table=11
                     /translation="MKYLMFVTAILGCLHLSAQEQGAEWRAPSNSSKAYGTYRNKLTVP
                     PYGLARVTALIGKIPYAEDDSSPMSNKDYMSLSLREKFTYHMIHMEINAQNCDVRPPIQ
                     DEDKKIFGMMADGFSESVWSDRQVKFMISNRDSVMALIKESTDRSKRMGANYKMAIVEI
                     NGRELIPYIIEVYNRDKKDRDLLTLLMLLMKNNEYRPFMESQSFRKLYGADANYQSYID
                     YNKANEDLIIKRATDYYNTVRK"
     gene            2777..3616
                     /locus_tag="B5D63_RS00830"
                     /old_locus_tag="SAMN04488128_101165"
     CDS             2777..3616
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1226: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217720.1"
                     /locus_tag="B5D63_RS00830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488128_101165"
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="WP_078666894.1"
                     /transl_table=11
                     /translation="MSGFLTYCLQRFTPVLLLLAGVNAVNGQSQRFVHVAAGDYSLGRK
                     GYINNPLRTVHTNGFDIAATELTNADFEKFVQATGYVTDAERRKNALVFIPGLREFRWL
                     EDSTAFWRYPNGITRGGIADKMDHPVTTISYRDAIAYCDWAGVRLPTLDEWEIASRAGA
                     PTTYFWGNEMTPLSSYANVWKGRDHLQADSTDGYMYTSPVASFKPNPWGMYDVYGNVFE
                     YCEGRLPTDKAGSRVVHARGGSWWCSKNACSFFNSVDIGSVKRGASFSNLGFRVVKK"
     gene            complement(3829..4200)
                     /locus_tag="B5D63_RS00835"
                     /old_locus_tag="SAMN04488128_101166"
     CDS             complement(3829..4200)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626750.1"
                     /locus_tag="B5D63_RS00835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488128_101166"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_078666895.1"
                     /transl_table=11
                     /translation="MYVKKLPELDYGVATTIKALGGKWKACILDCIHLGIRRPSELCRN
                     IPGASLRVLSQQLKELEACHIVYKKVYAEVPLRVEYYLTSWGESLWPVIVAMNEWGNAY
                     LHHHCDSLPQPNAAPCEES"
     misc_feature    4374..4389
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 20.69"
                     /tool="antismash"
     misc_feature    4433..4446
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 19.56"
                     /tool="antismash"
     misc_feature    4514..4527
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.63"
                     /tool="antismash"
     gene            4529..4726
                     /locus_tag="B5D63_RS35675"
                     /old_locus_tag="SAMN04488128_101167"
     CDS             4529..4726
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012392.5"
                     /locus_tag="B5D63_RS35675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488128_101167"
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_143312717.1"
                     /transl_table=11
                     /translation="MVLSVCLGIPYSATSINFRWKRRGNYCREGEQVSTVAYLLEYTSP
                     NNFSTAFRKRFGVSPGKFKG"
     gene            4789..4986
                     /locus_tag="B5D63_RS00845"
                     /old_locus_tag="SAMN04488128_101168"
     CDS             4789..4986
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B5D63_RS00845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN04488128_101168"
                     /product="hypothetical protein"
                     /protein_id="WP_078666897.1"
                     /transl_table=11
                     /translation="MNLSKSLLGAILAGITLQTAPSCNEPKAPATREEKKETKGDTVPA
                     KQDTSKPQLAGDGCPACGMG"
     gene            5001..6143
                     /locus_tag="B5D63_RS00850"
                     /old_locus_tag="SAMN04488128_101169"
     CDS             5001..6143
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790156.1"
                     /locus_tag="B5D63_RS00850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488128_101169"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_235021434.1"
                     /sec_met_domain="DUF692 (E-value: 6.1e-26, bitscore: 88.0,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPELISTVACNLDNNILAACLPLMEAARVEAIEWSFDALYAVENV
                     PAWFEELLTAFSQENRLIGHGVFFSLFSGRWLPEQQQWLDHLKTVSRHYRFDHVTEHFG
                     FMTGKDFHHGAPLNIPYTPVTLNIGRDRLKRMQDACECPVGLENLAFSYSLEEVKKHGD
                     FLNELLEPVNGFIILDLHNLYCQAHNFDLSFDALKALYPLHRVREIHISGGSWHESEAA
                     PGKTVRRDTHDDAVPEVVFNYLDKAIDLCPSLKYVVLEQLGTGLATEESRSAFGNDFLR
                     MDQLLKNKNTNTAPLNTFLPQALRIPAQAREDETLYRQQLELSQILETAASFEATMKAL
                     QASSLAHSAWQIENWDPAMIETAAKIAQKWKRQTPDRRIT"
     gene            complement(6147..6473)
                     /locus_tag="B5D63_RS00855"
                     /old_locus_tag="SAMN04488128_101170"
     CDS             complement(6147..6473)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B5D63_RS00855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN04488128_101170"
                     /product="hypothetical protein"
                     /protein_id="WP_143312718.1"
                     /transl_table=11
                     /translation="MKIMLMLMTAFMISSCATTYNSRFTSADVKPGISKQAVIARFGKP
                     FKKSFEQIGDVLYEDFYYKETIYKDYWFEINNILHFQDGKLVSLEQGEEKKLYQQVPVV
                     RVQR"
     misc_feature    complement(6458..6472)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 20.77"
                     /tool="antismash"
     gene            6550..6726
                     /locus_tag="B5D63_RS34520"
                     /old_locus_tag="SAMN04488128_101171"
     CDS             6550..6726
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B5D63_RS34520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN04488128_101171"
                     /product="HAD family phosphatase"
                     /protein_id="WP_143312719.1"
                     /sec_met_domain="HAD_2 (E-value: 3.5e-05, bitscore: 20.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTNKIKCVIFDCDGVLVDSEIIGIHVLPDLAAQYGVTMDEQEAV
                     RVMSGRNLRRGGR"
     gene            6716..6862
                     /locus_tag="B5D63_RS34885"
     CDS             6716..6862
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B5D63_RS34885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_159455846.1"
                     /transl_table=11
                     /translation="MEDSLAGIQAAKAGGFRALGYASEHSAAYLQEAGAEIFYSMKELP
                     RFL"
     gene            complement(6867..7589)
                     /locus_tag="B5D63_RS00860"
                     /old_locus_tag="SAMN04488128_101172"
     CDS             complement(6867..7589)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0030552 - cAMP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1133:
                     Transcription regulator, crp"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219703.1"
                     /locus_tag="B5D63_RS00860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488128_101172"
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_078666899.1"
                     /transl_table=11
                     /translation="MSLSGLFPIDKWNFRSRSVLSNLPEEERAALTSLMTTQRYNKGEV
                     IFREAAPAFGIYYIQKGKVKKYRTDKAGKEQIIYVANTGELVGYHAILAGGKYPDSAAT
                     LEESTIAFIPKDDFLQLLARSASLARRLLKTLSHEFTVLTNSISAFTHRSVQERLAITL
                     IVLREKFKDETPAGEDILIQVPRTDLANMVGTGVENVTRFLSAFKQAGVLTISGKKICI
                     TDVKKLVDISGCGEPLPS"
     misc_feature    complement(7608..7621)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.76"
                     /tool="antismash"
     gene            7638..8318
                     /locus_tag="B5D63_RS00865"
                     /old_locus_tag="SAMN04488128_101173"
     CDS             7638..8318
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0030552 - cAMP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1133:
                     Transcription regulator, crp"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629011.1"
                     /locus_tag="B5D63_RS00865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488128_101173"
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_235021491.1"
                     /transl_table=11
                     /translation="MKGAFPIHQWDFSNESVLVDLPQEVYDRLTAHKTERVYAPHEILF
                     REGAFPSGIFYIVKGKVKKYKANREGREQIVNLAHTGQLVGYHAVLSEERYPDTAAALE
                     KSTIAFIPKEDFLAALDQSPLLTNRLLTTLSHEFAVLVNNLTMFAQKTVRERLALQLLI
                     IREKFRDDVISLNREDLANLVGTARENVIRVLTAFREEGLVETRGRKIIIRDVDKLMAI
                     ASYP"
     gene            complement(8320..9054)
                     /locus_tag="B5D63_RS00870"
                     /old_locus_tag="SAMN04488128_101174"
     CDS             complement(8320..9054)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF018604.5"
                     /locus_tag="B5D63_RS00870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488128_101174"
                     /product="YoaK family protein"
                     /protein_id="WP_078666901.1"
                     /transl_table=11
                     /translation="MLRHTGKKRSYKHNLRLAVLLCFTAGMVNAAGLFAFAVLTTNVTG
                     HAALLAHKLASGDFRAARMVGLWLLLFLAGAFFSSIFSGKTGPFRRWGYVIPILCEMII
                     LILVGTFGHTYDQSILQTEYFAGSLLFAMGMQNALVSTISGYVVRTTHLTGMFTDLGID
                     LHSVLFHPHQHNSDVRKKIILRLAIIFFFLGGGIAGGYAFLRLSYYTFYLPAGVLVIAL
                     FYDLFRVRLRKMVRRMHTPATG"
     gene            complement(9070..9291)
                     /locus_tag="B5D63_RS00875"
                     /old_locus_tag="SAMN04488128_101175"
     CDS             complement(9070..9291)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B5D63_RS00875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN04488128_101175"
                     /product="hypothetical protein"
                     /protein_id="WP_078666902.1"
                     /transl_table=11
                     /translation="MVTERHISDIIEALKQAEDKQQVTQIVSGITHDDIAAQLQSWLET
                     QSPLEWDSTQWSSFRYALICLRGCPELQ"
     gene            complement(9303..9902)
                     /locus_tag="B5D63_RS00880"
                     /old_locus_tag="SAMN04488128_101176"
     CDS             complement(9303..9902)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025033.5"
                     /locus_tag="B5D63_RS00880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488128_101176"
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WP_078666903.1"
                     /transl_table=11
                     /translation="MNEITASPTWYVNDAAFDWLYPQRIQQLSRRHWTPVEVARKAARF
                     LSAGSGDRILDVGSGVGKFCLIGAHHYPESTFYGVEQRRELHNFARSAQRLTGVENAQF
                     IHGNVTQIDFDDFDHFYFYNAFFENLAEDGHIDQDTEYSASLYHYYCRQMFQGLAHKPG
                     GTRVVTFHSLEDEMPPSYQLVDASVDFLLKMWVKRS"
     misc_feature    complement(10067..10083)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 24.06"
                     /tool="antismash"
     gene            10151..10762
                     /locus_tag="B5D63_RS00885"
                     /old_locus_tag="SAMN04488128_101177"
     CDS             10151..10762
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /locus_tag="B5D63_RS00885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488128_101177"
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_078666904.1"
                     /transl_table=11
                     /translation="MPTVQLYDDTALFKKVALGDETAFRTLFDRYWDTVYGVALALTKS
                     ATMAEEMAQDVFVKLWLKRDKLAAVENFEGYLFMIARNHIFNTFRKKIQLASFTDHLPD
                     YAAQHLTTADTPDKRLLCHELETLVAKAVDQLPPQQRSVYCMSRQQGLSQEEIADALDI
                     SRHTVKSHMNKALHLIRNYLRQHATIETLLLFYFMAMTAR"
ORIGIN
        1 gaagcaacgc actggaactg ttgaaacgat cgcccggcgt gatggtagat cccgccaaca
       61 gcatttccct gaacgggaaa tcgggcgtaa cggtgtatat cgacggcaag cccagctaca
      121 tgcagggcga cgcgctggca gcattactca aatcattaca gtccgccaac atccatagca
      181 tagaaatgct tcccaatccg tccggtaaat acgatgcagc cggcagtggc ggtattatca
      241 atatccgcct gaagaaaaac accgcctccg ggcttaacgg ggatgtcgcc gctggtatac
      301 atttcgggca aacaccaaaa acggaaggag cgctgaacct caactaccgc accggcaaat
      361 tcaacctgta cggtaactac aaccactact tcgggtatca caatatggaa tatgattttt
      421 accgggtaca ggaaggagag atcatcgata accgtacgaa agatgtggac atgcgcaacc
      481 cggttaactt taaactggga gcagattata acatcagccg ccgccatact gtcggggtaa
      541 tgatgaacgc caatctgtac ttcggccccg ggatgaccaa taccaccact tatctgtcgg
      601 atgcagccac cgggcaactg acctccatgc tgaaggctac caatgactat tattcccaaa
      661 aacagaactg gaaaaattat aatgtcaact atcagtacaa agatacctcc ggccgcctgt
      721 ttaccaccga ccttgattac ggttattacc gggcccgtat caaaaatatc ctgtataacc
      781 attttctgaa agcagacagt gtcaccgcaa cagatcaata cacactgcgt acttttaaca
      841 acagtgatat caatatctat ggtattaagg ccgattatga acagccggtg tcccgcggta
      901 aaatttatgc aggcgttaag gccaatgccg tggtgagtga caaccgggtg cagatttttg
      961 atgtgagaga caaaacggat tacctgaacg aacgccggag caatactttc cgctacgatg
     1021 aaaaggtgta tgctgcttat gccatggctg atcagttgtg gggaaaatgg aagctgcagg
     1081 cagggcttcg ggtggagcag acagcagcgg atggcgtgct gcacgctaaa agccaggcta
     1141 cggctaaaga taccacgcag caggtgaaca accgttacct cgactttttt ccatccatgc
     1201 agctatcttt tcatccgtcg gagctgcatc acttctccct gcagtacaac cggaaaatcg
     1261 atcgccctac ctatacagat ctgaacccgt tcgcttacca gctggatgaa ctctcctcct
     1321 ggcaggggaa cgcttttctg cggccacagt attccaacag tgttactgtg ggttataacg
     1381 ctaccggtat tctgtcagcc tctttaacgt atactcgtcg ccgtgatatg ttcgtgtcgg
     1441 tatcggacag cattgatgga aacaaaatgg tgatgacgcc gaagaacgtg ggctcacagc
     1501 atttactggc gctcagcctg tcctccaacg tgtcgccgtt gccgtggtgg agcatttcag
     1561 ctaatatgaa cgcattttac aagcaaaacc tgctcacctt cgatgagcgc aggcgggcaa
     1621 acctcagcat caataccgta caactaaatg tgcaacaggt attcgatctt gataaaaaga
     1681 cgcaggcgga agtcagtggt aactaccagt cgcccgatat ctacggtggc ttccagcgaa
     1741 gtaaacataa ctggcaggtg aacgtcggtc tgcagcggaa agtcctgaag ggccagggca
     1801 ccgtgcgcat cggtatcaac gacttctttc agacggcacg ctggtacggc gtccgcgact
     1861 tcgacggcct gtactatcgt aacaagggat atgacgattc acgtcagtta aggctaggct
     1921 tcagttaccg gtttggaaac gtgaagctgg caaaagtcag ggaacgcagc tccggtctgg
     1981 aaagtgaatc ccgaagagtt aaataatgta gagatgtatt attttagcag tctaaacacg
     2041 aatacatgaa atatctgatg tttgtaaccg ccattttagg atgtttacac ctctctgccc
     2101 aggaacaagg ggcggaatgg agagccccca gcaattctag caaggcctat ggaacttaca
     2161 ggaacaagtt aacggtaccg ccctatggcc tggccagggt aacagcgctg atcggaaaga
     2221 tcccttatgc agaagatgac agttccccta tgagcaacaa agattatatg tccctttctc
     2281 ttcgggagaa gtttacctac catatgatcc acatggaaat caacgctcag aactgtgatg
     2341 tacgtccacc tatccaggac gaagacaaaa aaatattcgg catgatggcg gacggtttta
     2401 gcgaaagcgt gtggagcgac cggcaggtca agtttatgat cagcaaccgc gattccgtga
     2461 tggcgctgat caaagagtct accgaccgca gcaaaagaat gggcgccaac tacaaaatgg
     2521 ccatcgtgga aatcaatggc agggagctga tcccttatat catagaggtg tacaacaggg
     2581 acaagaaaga ccgcgacctg ctgacgttat tgatgctgct gatgaagaac aatgaatata
     2641 gacccttcat ggagtctcag tcgttccgta agttgtatgg agcggatgcc aactaccagt
     2701 cgtatatcga ctacaacaag gccaacgaag acctgattat caaaagagcc accgattatt
     2761 ataataccgt ccgtaaatga gcggcttttt aacatattgt ctgcaacgat ttaccccggt
     2821 cctgctgttg ctggccgggg taaatgctgt taatggccag tcgcagcgct tcgtgcatgt
     2881 ggcggccggc gactattccc tcggcagaaa ggggtatatc aacaatccgc tgcggacggt
     2941 tcacaccaac ggtttcgaca ttgctgccac tgaactgacc aacgccgact ttgagaaatt
     3001 tgtgcaggcc accggttatg ttaccgatgc agagcggcgg aaaaacgcgc tggtattcat
     3061 tcccggcctg cgtgagtttc gctggctgga agatagcacc gctttctggc gctatcccaa
     3121 cggcatcacc cggggtggca ttgcagataa gatggaccat cctgtaacaa ccatcagtta
     3181 ccgcgacgcc attgcctact gcgactgggc gggcgtaagg ttacccaccc ttgacgaatg
     3241 ggagatagct tcccgtgctg gtgcgcctac cacctatttc tggggaaatg aaatgacgcc
     3301 gctttccagc tatgccaacg tatggaaggg ccgcgaccac cttcaggccg atagcactga
     3361 tggttatatg tacacttctc ccgtggccag ttttaaaccc aatccctggg gaatgtatga
     3421 tgtatatggc aacgtcttcg aatactgcga aggccgcctg ccgacagaca aagccggcag
     3481 cagggtagtg cacgcccgcg gtggttcctg gtggtgcagt aaaaacgcct gcagcttttt
     3541 taattctgtg gacataggta gtgttaaacg cggagcgtcg tttagtaatc tggggttcag
     3601 ggtggtgaag aagtagttta atttccacaa acggtaaccc ccactttcca ttttcgttga
     3661 taccttcccc acatcaacaa atatgtttgg atgtgagcac acagacggct tccgcgaagt
     3721 tgtatgccga tgagtggata gactacatgc acctggtgaa actcaatggg agtggaagat
     3781 tgtcaatgtg ttgtggcagt tcaatcatca ggaagaacat ggctaagctc acgattcttc
     3841 acagggagcg gcgttgggtt gtggcaggct gtcgcagtga tggtgcaggt aggcattgcc
     3901 ccattcgttc atggccacga taacaggcca cagggattca ccccagctgg tgaggtagta
     3961 ttccacccgt agcggtactt ccgcatatac ttttttataa acgatatgac aggcttccag
     4021 ttccttcaac tgctggctga gcacccgaag agaagcaccg gggatattgc ggcaaagttc
     4081 gctgggacgc cgtatgccca gatggatgca gtccagtatg caggctttcc atttgccgcc
     4141 gagggctttg atggtcgtgg caacgccata atccagttcg ggtaactttt tgacatacat
     4201 aaggcacggt tacatttggt gcttcaaata tatccatttc ggccggcgcg gcctataatg
     4261 ataaatttat ccttatgggc gcagatcagt agctgcctgc tgctatgata cagcactaaa
     4321 taatacacga taatttgggg cttgttaatt aacgcctcct tcctttatct ttacgtgcaa
     4381 atcattatcc atacctgtca gttaccaagt tcgggaaata tagagaaact ttattttatt
     4441 aaagaactca tagataaaga accggaaacg gaccattctc tccgtaagct gagccgccag
     4501 gcgggattaa acgtttttaa actaaaaaat ggttttaagc gtgtgtttgg ggataccgta
     4561 ttcggctaca tccatcaact tcagatggaa aaggcgcgga aactattgca gggaaggaga
     4621 acaggtcagc acagtagcct atctgctgga atataccagc cccaataatt ttagtacagc
     4681 cttccgcaaa aggttcggcg tgtctcccgg aaaattcaaa gggtaacatt ttgtcctggt
     4741 attccgttta acgattaaat ctttttcatt cattcaaaag cccaaataat gaatctgtca
     4801 aaatcactgt tgggagccat cctggcgggt attacgctcc aaaccgcgcc ctcctgtaat
     4861 gaacccaaag ctcctgccac ccgggaagag aaaaaagaaa caaaaggcga caccgttccg
     4921 gcaaaacaag acacgtcaaa gccacagctg gcaggcgacg gctgccccgc ttgcggaatg
     4981 ggttaagtaa aacgtgaata gtgccggaat taatttcaac agtagcctgt aatcttgaca
     5041 acaacatcct ggccgcctgc cttcctttga tggaggcggc cagggttgaa gccatcgaat
     5101 ggtccttcga tgcgctgtat gccgtggaga acgtgcctgc ctggtttgaa gagctgttga
     5161 ccgccttcag ccaagaaaac aggttgatcg gtcatggtgt ctttttctct cttttttccg
     5221 ggagatggtt gccggagcaa caacaatggc tggaccatct gaagaccgtc agccgtcatt
     5281 accgcttcga tcatgtgact gaacattttg gttttatgac cggcaaagat tttcaccacg
     5341 gcgcaccatt gaatattcct tatacgccgg tgacgctcaa tatcgggcgc gatcggctga
     5401 agcgtatgca ggatgcctgc gaatgcccgg taggattgga gaatctggct ttttcctatt
     5461 cactggaaga agtaaaaaaa catggcgatt tcctcaacga gttactggaa ccggtcaatg
     5521 gatttatcat cctcgacctg cataatctct actgccaggc ccataatttt gatctttctt
     5581 tcgatgcgct gaaagcattg tatcctctgc accgtgtccg ggaaatacat atttccggtg
     5641 gcagctggca cgagtcggaa gccgcgccag gaaagactgt ccgccgcgac actcacgatg
     5701 atgcagtacc tgaggtcgtc tttaattatc tcgataaagc gatagacctg tgtccctcgc
     5761 ttaaatatgt ggtattggaa cagttgggga ccggactggc cacggaagaa agccggtcag
     5821 ccttcggcaa cgatttcctc cgcatggacc agcttcttaa gaacaaaaac acaaacaccg
     5881 ccccgctgaa cactttcctg ccgcaagcgc tgcggatacc ggctcaagcc cgggaagacg
     5941 aaacgctgta ccggcagcag ctggaactgt cgcagatcct tgaaacagcg gcttctttcg
     6001 aagccaccat gaaagcgctg caagcgtctt ccctggcgca ttccgcctgg cagatcgaaa
     6061 actgggaccc tgctatgatt gagaccgcgg cgaagattgc acagaagtgg aagcggcaaa
     6121 cgccggaccg ccgaattacc tgaagactag cgttgtaccc tcactacagg aacttgttga
     6181 tataacttct tctcttctcc ctgctccagg gaaaccagtt tcccgtcctg gaaatgcagg
     6241 atgttattga tctcgaacca gtaatctttg tagatggttt ccttgtagta aaagtcttca
     6301 tacagtacat ccccgatttg ttcgaagctt tttttgaaag gcttcccaaa gcgggcaata
     6361 acggcttgtt tactaattcc cggttttacg tctgcggaag tgaatcgact gttgtaagtg
     6421 gtggcgcagc tacttatcat gaaggctgtc attagcataa gcatgatttt cataacactg
     6481 gtattgggtg atactgaatg taagaaataa ccgtaccaca attatcttta tggtacgaac
     6541 aacagctata tgagtacaaa caagataaaa tgcgtcatat tcgattgtga tggcgtcctg
     6601 gtggacagtg aaattatcgg tatccacgtg ttaccggacc tggcggcgca atacggcgtg
     6661 acgatggatg aacaggaggc ggtacgtgtg atgagcggca ggaacctgcg ccgtggtgga
     6721 agatagcctc gctggcatac aggcggctaa ggccggcggt ttccgggcat tgggatatgc
     6781 cagtgaacac agtgctgctt atctgcagga ggcaggtgcg gagatatttt actcaatgaa
     6841 ggagttaccc cgtttcctgt aagtggctac gacggtaacg gctcaccgca gccggaaata
     6901 tccaccagtt tttttacgtc ggtgatacag atttttttac cggagatggt cagtacaccg
     6961 gcttgtttga aggcggataa aaagcgcgtc acattttcta cgccggtgcc taccatattg
     7021 gccaggtcgg tgcggggcac ctggataagg atgtcttcac ccgccggtgt ttcatcttta
     7081 aacttttccc gtaaaacgat cagggtgatg gccagccgct cctgtacgga acgatgggtg
     7141 aaagcggaga tactgttggt cagtacggta aattcatgac tcagcgtttt aagcaaccgt
     7201 cgtgcgaggc tggcggagcg ggccagcagc tgcaggaaat catctttagg gataaaagcg
     7261 atggtgcttt cttccagcgt ggcggcgctg tcggggtatt tgcctcccgc caggatggca
     7321 tgataaccta ccagttcacc ggtgttggcc acatagatga tctgttcttt ccccgcttta
     7381 tccgtccggt atttttttac ctttcctttt tgaatatagt agatgccgaa ggcgggcgca
     7441 gcttcccgga aaatcacttc tcccttgtta taacgctggg tggtcatcag tgaggtgagg
     7501 gcagcacgtt cctcttccgg caggttgctt aacacggacc gggagcggaa attccactta
     7561 tcgatgggaa aaaggcctga taaactcata gcaggacaaa ggtaaatttt aattttaaaa
     7621 tgctagtttt gaccgcaatg aaaggcgcct tcccgataca tcagtgggat ttcagcaatg
     7681 agtctgtgtt ggtggacctc ccgcaggagg tgtacgaccg gcttacggcc cataagaccg
     7741 agcgggtgta cgctccgcat gaaatcctct tccgcgaagg ggctttccct tccgggatat
     7801 tttatattgt caaaggcaaa gtaaaaaagt acaaggcaaa cagggaaggg agggaacaga
     7861 tcgtgaacct ggcccatacc gggcaactgg taggttatca tgcggtgctc tctgaagaac
     7921 gttaccccga caccgcggca gccctcgaaa agagcaccat cgcttttatc cctaaagaag
     7981 attttctcgc cgcactggac cagtcgccgt tgctgaccaa ccggctgctg acaacgctca
     8041 gtcatgagtt tgccgtactg gtgaataacc tgaccatgtt cgcccagaaa acagtaagag
     8101 agcggttggc gctgcagcta ttgattatca gggagaagtt cagggacgat gtcatcagtt
     8161 tgaaccggga agacctcgcc aacctggtag gaacggcccg tgaaaatgtg atccgcgtgc
     8221 tgacagcttt cagggaagaa gggctggtgg aaacacgggg ccgcaaaatc attatccgcg
     8281 atgtggataa actgatggcg atagccagtt acccttgagt tacccggtcg caggcgtatg
     8341 catacgccgc accattttcc gcagccgtac gcggaaaaga tcataaaaca aagcgatgac
     8401 cagcacgcct gccggcagat aaaaagtgta gtaagacagc cggagaaatg catacccgcc
     8461 cgcaatacct cctcccagaa aaaagaagat gatggccagc cggagaataa tttttttcct
     8521 gacatcgctg ttgtgctgat gcggatggaa tagtacgctg tgaaggtcga tcccgagatc
     8581 ggtgaacata ccggtcaggt gcgtggtgcg caccacatac cctgagatgg tggacaccag
     8641 cgcattctgc atacccatgg caaacaggag actgcctgca aaatattctg tctgtaaaat
     8701 actttggtca taggtatgcc cgaatgtgcc caccagtata aggataatca tttcgcagag
     8761 gatgggtatc acataacccc atcgcctgaa agggccggtt ttgccggaga aaatgctgga
     8821 gaaaaatgcg cctgcgagga aaagcaacag ccataaccct accatgcggg ccgccctgaa
     8881 atcaccgctg gccagcttat gggccagcag tgcggcgtga ccggtgacat tggtggtaag
     8941 aacggcgaag gcaaaaagcc ccgccgcgtt taccatccct gcggtaaaac acaacagtac
     9001 cgccagtcgt aggttatgct tgtaacttct tttttttccg gtatggcgca gcatggacca
     9061 atagcgcttt tattgcagtt cgggacatcc ccgcaggcag atcagcgcat aacgaaagct
     9121 gctccattgg gtgctgtccc attccagtgg ggattgagtt tccagccatg actgcagttg
     9181 agctgctatg tcatcgtggg taatgccgga aacgatctgt gttacctgtt gtttgtcttc
     9241 cgcctgtttt agtgcttcaa taatgtctga tatatgtctc tctgttacca tgataatatt
     9301 ttttatgatc gttttaccca cattttcagg aggaagtcca cgctggcgtc taccagctga
     9361 tagctgggcg gcatctcgtc ttccaggctg tggaaagtga ctacccgtgt accgcccggc
     9421 ttgtgtgcca gcccctggaa catctgccgg cagtagtagt ggtacaggct cgcggaatat
     9481 tccgtgtcct ggtcaatatg cccgtcctct gccaggttct cgaagaaggc gttgtaaaaa
     9541 taaaagtgat cgaaatcgtc aaagtctatc tgggtaacat tcccatggat aaattgggcg
     9601 ttttcaacgc cggtcagccg ttgtgcggag cgggcgaaat tgtgcagttc cctgcgttgt
     9661 tccacaccgt agaaagtgct ttcggggtag tggtgtgcac cgatcaggca gaatttgccg
     9721 acgccgctgc ctacgtcgag gatgcggtcg cctgagccgg cagacaggaa acgggccgct
     9781 ttgcgggcta cttccaccgg tgtccagtgc cgtcttgata gttgctgaat acgttgcggg
     9841 tacagccagt caaaagctgc atcatttaca taccacgtcg ggcttgcggt tatttcgttc
     9901 ataccgggtg caaagttagc cagccacggc gtcggtagcg ttgatacttt tcaggaaaaa
     9961 agctgcaaaa agtcactttt tgagatgttc ccggaggata atatctgcga agaagcggat
    10021 aatttctaag aagggcgtac tttgatttcc ccctaaattt aataccttgg aagaaattta
    10081 caaccaaaat aattgatcgc ataccggatg gttttttaag gatgaccggc cggttttatc
    10141 tgtctgaaca ttgccaaccg tccaactata tgatgatacg gcccttttta aaaaagtggc
    10201 cctcggtgat gaaacggctt tccggacact cttcgaccga tattgggata ccgtgtacgg
    10261 cgttgctttg gcactgacca aatcggctac catggcggag gagatggcgc aggacgtttt
    10321 cgtgaaactg tggcttaaac gggataagct ggcagctgtc gagaattttg aaggctatct
    10381 gtttatgata gcgcgcaacc atattttcaa tactttccgt aaaaaaatac agctcgcttc
    10441 ttttacagac catttgccgg actatgccgc ccagcacctg acgacggccg atacaccgga
    10501 caaacgcctg ctatgccatg aactggaaac actggtggcc aaagcggtgg atcaactgcc
    10561 gccgcagcag cggtcggtat attgcatgag ccggcaacag ggccttagcc aggaggagat
    10621 cgcggatgct ctggacatct cccgtcatac agtgaaaagt catatgaata aagcattaca
    10681 ccttatccgc aattatctcc ggcaacatgc taccatcgaa acgctgcttc tcttttattt
    10741 tatggcgatg accgcccgtt gaaaaaaaaa ttttacttcc aactcatacc accacctttt
    10801 accgatgtct ttatagacac agcaaccacc attgcccatg gaacaggctc atttgaaaac
    10861 actgctgcag cgttatctcg ataacaacct ttctccgcaa ggactgaagg aactgatcgt
    10921 gcacctgcaa caggacacac aacacgatac gcttttacag gtggtgcagc aggcgctggc
    10981 cagcggtgct tatgcggcac ctgccgacgc cgcgcagaag gatactattt tccggaacat
    11041 cctgcaaaag gcggcggagc aggaagcagc tacggcgctg cagccggtaa aacgccgcac
    11101 cagcctgtgg agagccgctg tagccgccat ggtgattgtg ctg
//
