LOCUS       NZ_LT745763            20867 bp    DNA     linear   CON 26-JUL-2024
DEFINITION  Virgibacillus dokdonensis strain Marseille-P2545, whole genome
            shotgun sequence.
ACCESSION   NZ_LT745763
VERSION     NZ_LT745763.1
KEYWORDS    WGS; RefSeq.
SOURCE      Virgibacillus dokdonensis
  ORGANISM  Virgibacillus dokdonensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Virgibacillus.
REFERENCE   1
  CONSRTM   Urmite Genomes
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2017) URMITE, 27 Bd J Moulin, 13385 Marseille
            Cedex 05, France, 13385, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900166595.1-RS_2024_07_26
            Annotation Date                   :: 07/26/2024 01:28:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,350
            CDSs (total)                      :: 4,265
            Genes (coding)                    :: 4,169
            CDSs (with protein)               :: 4,169
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 7, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 64
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 26 of 96
            Pseudo Genes (multiple problems)  :: 22 of 96
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:20:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1938679
            Orig. end                         :: 1959546
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT745763.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20867
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="2"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20867
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20867
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10867](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10867
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            265..471
                     /gene="spoIIIAC"
                     /locus_tag="B2C77_RS10590"
     CDS             265..471
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="spoIIIAC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008637060.1"
                     /locus_tag="B2C77_RS10590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="stage III sporulation protein AC"
                     /protein_id="WP_021291150.1"
                     /transl_table=11
                     /translation="MFTDATILFQIAGIGIIVALIHTILKQMGKEEIAQFATLIGFIIV
                     LVLVIDGLSDLFQQIKSVFLYQG"
     misc_feature    307..320
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.73"
                     /tool="antismash"
     gene            478..870
                     /gene="spoIIIAD"
                     /locus_tag="B2C77_RS10595"
     CDS             478..870
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="spoIIIAD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066288.1"
                     /locus_tag="B2C77_RS10595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="stage III sporulation protein AD"
                     /protein_id="WP_077703575.1"
                     /transl_table=11
                     /translation="MDILQIVTLGLVASLLYIILKNVNQTFAFFVVLLTGVVIFLSIIQ
                     QINHIFQLIQALGAKAQVDGMYLETILKIIGIAYIAELGANITKDAGLTSVANKIELAG
                     KLFILLLAIPIITAVIEAILQFIPTV"
     gene            920..2104
                     /gene="spoIIIAE"
                     /locus_tag="B2C77_RS10600"
     CDS             920..2104
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="spoIIIAE"
                     /inference="COORDINATES: protein motif:HMM:TIGR02829.1"
                     /locus_tag="B2C77_RS10600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="stage III sporulation protein AE"
                     /protein_id="WP_077703576.1"
                     /transl_table=11
                     /translation="MNGKQIGIILICTFVICLLGYEKAIAETTSNESDTVQNNMLEEIS
                     LEGVQAYWGKLVNEYGGYLPELEKTSVYEFIKNQDSLSPQSIIIGILEFLFHELILNGK
                     LLGSLLMLTLFSAMLQTMHNAFETSTVSKVAYFVVYIVLIFLALNSFYSAVTYTKDAID
                     AMSSFMIALLPLVLGLMASFGNIVAVSFFHPIIIFLIHAGGLIVANFILPLLFLSALLV
                     IVSSLTEHYKVTHLANLFKSIAVGTLGVFFTIFLSIMSVQGTASAIQDGVVMKTTKFIT
                     GNFIPVVGRTMTDAADTILSASLLLKNAIGIVGLIIILFIALFPALKIFAIAVIYKVAA
                     AILQPIADGPVITTLNTISKYIVFILACLLAVSFMFFLAIVIIIAASNVTLLLR"
     gene            2121..2741
                     /gene="spoIIIAF"
                     /locus_tag="B2C77_RS10605"
     CDS             2121..2741
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="spoIIIAF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093205.1"
                     /locus_tag="B2C77_RS10605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="stage III sporulation protein AF"
                     /protein_id="WP_077703577.1"
                     /transl_table=11
                     /translation="MQIVIDWVTQIILFILLATVIDLVIPANSMKKYMKLVVGLILILI
                     LLKPIFYIFQIDVQRAVESSLSGVFEEMNQNNSLDNSIKTQKNEIEATQDAYILEQMAV
                     QLKEIAEDPLHEAFQTEITDIQFQFVNGKDKTYENLDKVIVYVNELKNGEGAVRNVDDV
                     VIYSDSPDVSKENNELKDIENKLSDLWEMEQKNLILQWEGGAP"
     gene            2738..3367
                     /gene="spoIIIAG"
                     /locus_tag="B2C77_RS10610"
     CDS             2738..3367
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="spoIIIAG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093203.1"
                     /locus_tag="B2C77_RS10610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="stage III sporulation protein AG"
                     /protein_id="WP_077703578.1"
                     /transl_table=11
                     /translation="MIEKLKEFFQKDGKKPPKKLSYMIILALVSVMLLIIGNIFSTSSE
                     TDMEEPIQQPTEQASEPNSKETASPKEATTSNIEELESVYTKDLQTLINKIQGVSEAEV
                     MVNLESTSIKVYEKNLIKGQQTTEETDTNGGTREIEDGTEETQTVLVRQGEKEVPLLIQ
                     TKKPEVRGVFVVAKGADHATVKKWIVEAVSRVLDVPTHRVSVMPKN"
     gene            3381..3962
                     /locus_tag="B2C77_RS10615"
     CDS             3381..3962
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019378267.1"
                     /locus_tag="B2C77_RS10615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SpoIIIAH-like family protein"
                     /protein_id="WP_077703579.1"
                     /transl_table=11
                     /translation="MLKKQTVWLLTMLSLMILLSVYYMTSPDSEDLAYINDGQENAQEE
                     KAVPTDSEANDDAEVEEISNVGQDELFTTIRMELQDKRNMEKERLTDIVASSSASSAEK
                     DEALQEIDAIEDLASKEKILEETIVASANYKDVLVRSEDDSVTVQVKVEENLPKSEAVS
                     IIQMVKDELGDKKVNVSFLPSQGETEKDTE"
     misc_feature    complement(4205..4221)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 25.13"
                     /tool="antismash"
     gene            4344..4829
                     /gene="accB"
                     /locus_tag="B2C77_RS10620"
     CDS             4344..4829
                     /EC_number="6.4.1.2"
                     /GO_component="GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA]"
                     /GO_function="GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="accB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093199.1"
                     /locus_tag="B2C77_RS10620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="WP_077703580.1"
                     /transl_table=11
                     /translation="MLNVQEIQELIRLIDQTSIEEFKYETSGASISLKKSSTTGQVQPK
                     NIPEKNVSETVAQIESEKASAENTQDASSQKESTVDYDHEIVSPMVGTFYAAPSPEREN
                     FVSIGSKVDKSSVVCIVEAMKLFNEIEAEVEGEIVEILVENGELVEYGQPLFRVSTK"
     gene            4843..6204
                     /gene="accC"
                     /locus_tag="B2C77_RS10625"
     CDS             4843..6204
                     /EC_number="6.3.4.14"
                     /GO_component="GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA]"
                     /GO_function="GO:0004075 - biotin carboxylase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="accC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1090: acetyl-CoA carboxylase biotin carboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093197.1"
                     /locus_tag="B2C77_RS10625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="WP_077703581.1"
                     /sec_met_domain="RimK (E-value: 4e-06, bitscore: 24.4,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 5.2e-08, bitscore:
                     30.5, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 3.5e-10,
                     bitscore: 37.5, seeds: 12, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKKLLIANRGEIAVRIIRACKEMGIETVAVFSQADSEALHVQMA
                     DEAYCIGPTQSKDSYLNFTNIMSIATLTGVDAIHPGYGFLAENADFAEICRACNITFVG
                     PTPEAIQKMGIKDVARQTMEAANVPIVPGSDGIIHDVEEAKEIANQIGYPIIIKATAGG
                     GGKGIRVARTEEELTKGVEITQKEAETSFGNAGVYLEKYIEDFRHVEIQVLADKHGNVI
                     HLGERDCSIQRRLQKLVEEAPSPAITPEIREKMGEASVKAAKAVGYEGAGTIEYIFDRK
                     THQFYFMEMNTRIQVEHPVTELITGIDLIKEQIKIADNEVLAYSQSDISFNGWAIECRI
                     NAENPFKNFMPSAGKVDMYLPPGGLGVRVDAAVYPEYRIPPYYDSMIAKLITYGKTREE
                     AIHRMKRALDEFIVEGVHTTIPFHHKIMEHPVFINGDFNTKFLEENPITQDEDE"
     gene            6218..6616
                     /locus_tag="B2C77_RS10630"
     CDS             6218..6616
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093195.1"
                     /locus_tag="B2C77_RS10630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="WP_077703582.1"
                     /transl_table=11
                     /translation="MSEQPLLNVSDDSLGKVEIAPEVIEVITGLAASEIDGLYAMRGNF
                     ASGVAERFGKKAHSKGVKVELTENGIVIDLYVILQFGVSIPHVAQLLQTNIRQTLKNMT
                     ALEIDEINVHVVGIQMDYPEEATEKTNS"
     misc_feature    complement(6881..6894)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.22"
                     /tool="antismash"
     gene            7030..7410
                     /gene="nusB"
                     /locus_tag="B2C77_RS10635"
     CDS             7030..7410
                     /GO_process="GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /gene="nusB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010530946.1"
                     /locus_tag="B2C77_RS10635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transcription antitermination factor NusB"
                     /protein_id="WP_077703583.1"
                     /transl_table=11
                     /translation="MNRHEARKKALQIIFSIDIHEMQSIHEMEHLDEQVRKDAFLNLLV
                     NGVINYKVEIDQIISEKLENWSLERIASVEKAILRIAVFEIRYVEDIPVSVSINEAIEL
                     ANTFGDEKSGKFINGVLSRISD"
     gene            7555..8415
                     /gene="folD"
                     /locus_tag="B2C77_RS10640"
     CDS             7555..8415
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /GO_function="GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /gene="folD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098063.1"
                     /locus_tag="B2C77_RS10640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD"
                     /protein_id="WP_077703584.1"
                     /transl_table=11
                     /translation="MSAEIINGKNLSLALKEEMKKEVDALKIDGIHPQLTVILVGDHPA
                     SRSYVNGKEKACGETGIGSHIIELPKTVSESDLLKGIHRLNDDANVHGILVQLPLPVHI
                     NEQKVIETISPEKDVDGFHPINIGRMMTGMDTYLSCTPYGIITMLRSKNISIAGKHAVV
                     IGRSNIVGKPVAQLLINENATVTTCHSKTENLKQFTRIADILVVAVGKANIIDASYVKE
                     GATVIDVGMNRLENGKLTGDVDFDSVQEKASFITPVPRGVGPMTITMLLKNTIKAARRL
                     ENREG"
     gene            8405..9757
                     /gene="xseA"
                     /locus_tag="B2C77_RS10645"
     CDS             8405..9757
                     /EC_number="3.1.11.6"
                     /GO_component="GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA]"
                     /GO_function="GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /GO_process="GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="xseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066276.1"
                     /locus_tag="B2C77_RS10645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="WP_077703585.1"
                     /transl_table=11
                     /translation="MKDNYLTVTALTRYIKRKMDVDPHLRQVWLRGEISNFKHHSRGHM
                     YLTIKDDRSRIQAVMFAGNNRSLKFQPENGMNVLIKGEISVFEAYGQYQLYIQQMEPDG
                     IGALYVAFEQLKEKLEKQGYFDHYVKKNIPPLPKNIGIITSPTGAAVRDIITTIKRRYP
                     IVELTVIPALVQGEAAARSIKQAIEIANQKGDFDLLIVGRGGGSIEELWSFNEEIVAKA
                     IFHSNIPIISAVGHETDTTISDFVADLRAPTPTAAAEIAVPSSQDLKDKIATLKRMATK
                     DMQEKWKQQQKHLFRLKQSYAFRYPYQLLKQKEQELDGKIEQLQKAMLHTKEASNKSYE
                     HLWARFTVQHPGKQVERGKQQLQQLVKQQYMVMKQLHTDKVKKLHNEMDKLSLLNPLAI
                     MKRGFSIPYTSAGQIIKSVHAVEREDAISLRLADGLLKCKVIETKEESQND"
     gene            9750..9980
                     /locus_tag="B2C77_RS10650"
     CDS             9750..9980
                     /EC_number="3.1.11.6"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008637088.1"
                     /locus_tag="B2C77_RS10650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="WP_073004335.1"
                     /transl_table=11
                     /translation="MTEEELSFEEAMQQLEEVVEKLETGDVPLEKAISYYQDGMKLSKL
                     CTDKLNQVEEKMTKIMNEQGELEGFPLQEEE"
     gene            10001..10867
                     /locus_tag="B2C77_RS10655"
     CDS             10001..10867
                     /GO_function="GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019378275.1"
                     /locus_tag="B2C77_RS10655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_269467145.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 2.3e-58, bitscore:
                     195.4, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYLEEKKQFIDSALKKYMQALQVPPALKQSMTYSVEAGGKRLRPI
                     LLLASYEAFQKDITVALSSAAALEMIHTYSLIHDDLPAMDDDDYRRGKLTNHKAFSEAT
                     AVLAGDALLTYSFEIISKDDKLTAAQKAELIALLAEASGPKGMVAGQILDMEAEDRAIT
                     LQELERVHTLKTGELLRYAVHAGAYLGGATEKQLHELQLFASYLGLIFQVQDDILDVTG
                     DEVKLGKPVGSDALNLKSTYPKLLGINGAIEKKEQYLLKAKESLQAAGVENSNLMDLAI
                     YFGQRDY"
     misc_feature    complement(10875..10888)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.9"
                     /tool="antismash"
     gene            11565..12140
                     /locus_tag="B2C77_RS10660"
     CDS             11565..12140
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019378276.1"
                     /locus_tag="B2C77_RS10660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_077703586.1"
                     /transl_table=11
                     /translation="MKSHVKRSINFSLTIAVITFVLAAIFSVISSSILSGVAWFIGLFI
                     VFVIVFVGVVFDMLGIASTAANEAPFHAMASEKVTGAKEAVIIIRNADRFASFCNDVIG
                     DISGIVSGTASAIVVLQMANLLGHGEGSSVQIIISVVLTSIVAALTVGGKAMGKFFAIN
                     ASTKIIFFAGKVISIMENKLKISVLPKN"
     gene            12160..12996
                     /locus_tag="B2C77_RS10665"
     CDS             12160..12996
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002138285.1"
                     /locus_tag="B2C77_RS10665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="WP_077703587.1"
                     /transl_table=11
                     /translation="MSKKRLDVLLVEKSLAATREKAKRLIMAGLVFADQQRLDKPGMQV
                     DESINLTVKGKLLPYVGRGGLKLEKALKTFSLTVTDKIVMDIGSSTGGFTDCALQNGAS
                     LCYAIDVGYNQLDWKLRNDDRVIVMERTNFRYVTVGMLENGQPNFASIDVSFISLRLLL
                     PVLKHLLENNSDVVALVKPQFEAGKEQVGKKGVVRDSRVHQAVLRQIIDFAAQEKFQIL
                     DLTFSPITGGDGNIEFLLHLGWEKHKPATKDFSDHEIESLVSEAHEALARKKQNNK"
     gene            13093..13542
                     /gene="argR"
                     /locus_tag="B2C77_RS10670"
     CDS             13093..13542
                     /codon_start=1
                     /gene="argR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390305.1"
                     /locus_tag="B2C77_RS10670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transcriptional regulator ArgR"
                     /protein_id="WP_073004330.1"
                     /transl_table=11
                     /translation="MSKIQRHIKIRELITDNEIETQDELVDRLKNLGYNVTQATVSRDI
                     KELHLVKVATANGTYKYSLPADQRFNPLDKLKRLVMDAFVKIDYASHFIVLKTLPGNAH
                     AVGALIDQLDWEEIMGTICGDDTCLIICRTEADALTIKERFIEML"
     gene            13565..15286
                     /gene="recN"
                     /locus_tag="B2C77_RS10675"
     CDS             13565..15286
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019378279.1"
                     /locus_tag="B2C77_RS10675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA repair protein RecN"
                     /protein_id="WP_077703588.1"
                     /transl_table=11
                     /translation="MLTELSIQDFAIINKLSVTFNEGLTVLTGETGAGKSIIIDAVQLL
                     AGGRGSVEFVRHGAKKADIEGLFIIDHDSHPVYTICEQYGIESEDQQLVLRRTITANGK
                     SICRVNSKLVTLAVLREFGRTLIDIHSQHETQSLMNPEHHLDLLDLYQPELINKAKKEY
                     LKLYDQLSKLKNRYRKLSENEQETAQRLDLLQFQLRELEEAQLQPNEDKQLEEERNTLA
                     NFERIYSSLQLAYNSLYGEQKGLDWVNQAQIALQDNSNYDASLAQKAEDIANYYYGLEE
                     LTYDLGNQIETLQFDPERLNEIEARLNEINRLKRKYGPTVSDMLTYMAKIEEEVEEISN
                     KDSHISKLEQQIKEKAKDAFLEAQELRDIRIKAAEALTKEIHEELKDLYLGKTEFKVDF
                     AKNIEPQKELDKHLHKNGMDDVTFLISTNPGEPPKPLNKVASGGELSRIMLSLKKIFAR
                     HQGVTSVIFDEVDTGVSGRVAQAIAEKIYHISQKSQVLCITHLPQVAAMADTHKLIEKE
                     VMNQRTSTTVHELSQKLQINELARMITGTKLTDTAVDHAREMLEMAIQFKGKEHNL"
     gene            15567..16763
                     /gene="spoIVB"
                     /locus_tag="B2C77_RS10680"
     CDS             15567..16763
                     /EC_number="3.4.21.116"
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="spoIVB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466343.1"
                     /locus_tag="B2C77_RS10680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SpoIVB peptidase"
                     /protein_id="WP_077703589.1"
                     /transl_table=11
                     /translation="MNLRTKHNKILRFGICFMTMLALLALPVITSLFKAESRITQAATT
                     ATSMSPEDTSTLHQFTGLPIQQTKAQSKEEIKIIPGGQSIGVQLQTKGVLVVGHHLVKG
                     KTGRVSPGEKADIQVGDVILEIDGKEVKKMKDVKPFVKQAGQSKNVLDIKLKRGKKTIS
                     TELEPALDEKENNYQIGLYIRDSAAGIGTMTFYDPNSKKYGALGHVISDMDTQKPIEIN
                     NGSIVRSSVTSIEKGNNGIPGEKQATFSAAEDKIGSITKNTAFGIFGKLNQNLEELKKP
                     LPVALPNEVKEGPAKILTVLAGEEIKEYDVEVVSSVPQKNAATKGMVIQIKDKELLNKT
                     GGIVQGMSGSPIIQNGKIIGAVTHVFVNDPTSGYGVHIKFMLEEAGINTATSSPKKVS"
     misc_feature    17069..17082
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.97"
                     /tool="antismash"
     gene            17157..17933
                     /gene="spo0A"
                     /locus_tag="B2C77_RS10685"
     CDS             17157..17933
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /GO_process="GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /GO_process="GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /GO_process="GO:0051606 - detection of stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="spo0A"
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013084985.1"
                     /locus_tag="B2C77_RS10685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="WP_077703590.1"
                     /transl_table=11
                     /translation="MEKIEICLVDDNRELIKLMEDYFEEQSDIEVIGTAFNGRDCLTML
                     DDLEPDVLILDIIMPHVDGLAVLNTLRSSKENPPHVIMLTAFGQEEVMKKAVDLGAAYF
                     ILKPFDLDNLAEQIRQVQGSSSYSVGVSKVQRKERKKQDLEASITNIIHEIGVPAHIKG
                     YMYLREAITMVFNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDS
                     ISALFGYTISISKAKPTNSEFIAMVADHLRLEHKAS"
     gene            complement(18005..19354)
                     /locus_tag="B2C77_RS10690"
     CDS             complement(18005..19354)
                     /EC_number="2.7.2.4"
                     /GO_function="GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012576134.1"
                     /locus_tag="B2C77_RS10690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aspartate kinase"
                     /protein_id="WP_077703591.1"
                     /transl_table=11
                     /translation="MKVAKFGGSSVASAEQIKKVANIIKKDLQKKFIVVSAPGKQHKSD
                     QKMTDMFIQLWKASQQETSLEPYIDPIIKRFTEIVEQLGLSFIIVGEIKESINKLLHME
                     EAPISKLDALKAIGEDSSAKILSAYLTSIGLEARYVNPREAGIMVSDEPGNGKILPESF
                     EMIYNLRSTKGISVIPGFFGYTNAGKLITFSRGGSDITGSIIAAGVKADLYENYTDVDS
                     VYAVNPSIVDNPKKITTLTYKEMRELSYAGFSVFHDEALIPAYREKIPVCIKNTNNPQA
                     TGTFIVSEKKPSEKCVVGIASDTGFCSIYISKYLMNREIGFGRKVLSILEEEGISFEHA
                     PSGIDDLSIIIREKHLPLEKEKQVLKRIQKVLCPDLISIHRNLAMIMIVGEGLVRTIGV
                     AQKATSAISSANVNIEMINQGSSEVSMMFGIIADDLEKAVQSLYNAFFVN"
     gene            complement(19509..19760)
                     /locus_tag="B2C77_RS10695"
     CDS             complement(19509..19760)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017435225.1"
                     /locus_tag="B2C77_RS10695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF2627 domain-containing protein"
                     /protein_id="WP_077703592.1"
                     /transl_table=11
                     /translation="MIRIIAILIMFIPGLIAAIGIKLMRDTLFATFYPIFLHSSIQFII
                     GLILFLAGLAFIGGFIIHRDRKRQKNQQQKRNCYTNGD"
ORIGIN
        1 gaatagttta gaaacattta tggatatatc gtgtcttgga aaaaacgaga aagaaatatt
       61 gcttcaattt ggacaaacac ttggaaaaca tgattttctc caacagcaaa agcatattca
      121 gctggcagcc agtcatttgg aaagggagct ggaagccgct aatgaacagt atcaaagata
      181 cgggaaaatg acaaaaagtt taggtatatt atgtggtgtg tttatcgtat tgttgctttt
      241 atagtgtgaa aggagttctt ccctatgttt acagacgcga cgattttatt tcaaattgct
      301 ggaattggca ttattgttgc tttaatccac accattttaa agcagatggg taaggaagaa
      361 atcgcccagt ttgccacatt aattggcttt attattgttc ttgtattagt cattgatgga
      421 ctatcagatc tctttcagca gattaaatct gtttttcttt atcaagggta gtgtgcaatg
      481 gatattttac aaattgtaac attgggtcta gtagctagct tgctttatat cattttaaaa
      541 aatgttaatc agacatttgc attttttgtt gtattactta caggggtagt tattttcctg
      601 tccatcatac aacaaatcaa tcatatattt cagttgattc aagcattagg tgcaaaagca
      661 caagtagatg ggatgtattt ggaaaccatt ttaaaaataa ttggcatcgc ttatattgct
      721 gaattgggag cgaatattac aaaagatgca ggactaactt ccgtagccaa taaaattgaa
      781 cttgcaggaa aattatttat tttgttattg gctataccaa taatcactgc agtaatagaa
      841 gcgattttac aatttatacc tactgtatga aaagtattgt agtacgagaa ataaacctag
      901 gaaggagttt ttatcgtgca tgaatggtaa acaaataggg atcattctta tatgtacttt
      961 tgttatttgc cttctgggtt atgaaaaagc tatagcagaa acgacatcta atgaatcaga
     1021 tactgtacaa aataacatgc tagaagaaat ttctttggag ggggtacaag catactgggg
     1081 taaattagta aatgaatatg gtggatattt accagaactc gaaaaaacaa gtgtatatga
     1141 atttattaaa aatcaagatt ctttgtcacc tcaatctatc attattggta ttttagaatt
     1201 cttatttcat gaactgattt taaatgggaa gttattaggc tctcttttaa tgttaacttt
     1261 attttcggca atgctacaga cgatgcacaa tgcgtttgag acaagtaccg taagtaaagt
     1321 cgcttacttt gttgtatata tcgtgctcat ttttttggca ttaaatagct tctattcagc
     1381 ggtgacctac accaaagatg cgattgacgc gatgagtagt tttatgattg ctctcttacc
     1441 tttagtctta gggttaatgg cttcatttgg gaatattgtg gcggtttctt tttttcatcc
     1501 gattattatc tttcttattc atgcaggtgg tctcatcgtt gctaatttta ttttgccgct
     1561 cttgttttta tcggctttgt tagtgatagt cagtagctta acagagcact ataaggtaac
     1621 gcatttagcg aatctattta aatccattgc agttggcacc ttaggtgtat tctttaccat
     1681 ttttctaagc atcatgtcag tacaaggaac agcaagtgcc attcaagatg gggtagtgat
     1741 gaaaacaaca aaatttataa ccggaaactt tataccagtt gtgggtcgta cgatgacaga
     1801 tgccgcagat acgatattaa gcgcatccct attgttaaaa aacgcaattg gaattgtagg
     1861 gttaattatt atactgttta tcgctttgtt tccggcatta aaaatctttg caattgcagt
     1921 aatttataaa gttgctgccg caatcttaca gccgattgcg gatgggccag ttattactac
     1981 attgaataca attagtaaat atattgtttt tattcttgcg tgtttattag cagtatcatt
     2041 tatgtttttt ttggcaattg tcatcattat tgcagctagt aatgtaactt tgctcttaag
     2101 ataggggggg cgagcttgaa ttgcaaatag ttattgactg ggtcacacaa attattctat
     2161 ttatcttgtt agctacggtc attgatttag ttattccagc taattccatg aaaaaataca
     2221 tgaagctcgt tgtcggactt atccttatat taattttgtt aaagcctata ttttatattt
     2281 ttcaaatcga cgtccaacgt gcagtggaaa gctctctatc aggtgttttt gaagaaatga
     2341 atcaaaacaa ttctctagat aattccatta aaacacaaaa aaatgaaata gaagctacac
     2401 aagatgcata tattttagaa caaatggctg tccaattaaa agaaatagca gaagatccac
     2461 ttcatgaagc gtttcaaaca gaaattacag atatccaatt tcagtttgta aatgggaaag
     2521 ataagacata tgaaaatttg gacaaagtca ttgtgtatgt gaatgaatta aaaaatgggg
     2581 agggagcagt gcgaaacgtt gatgatgtag taatttattc agacagccca gatgtatcta
     2641 aagaaaataa cgaattgaag gacatagaaa ataagctatc cgatctatgg gaaatggagc
     2701 aaaaaaatct aattttacaa tgggagggag gggcaccttg atagagaaat taaaagaatt
     2761 ttttcaaaaa gacggaaaaa aaccacctaa aaaattgagc tacatgatca ttttagcctt
     2821 agtcagtgta atgttgttaa ttattggtaa tatcttttca acttcatcag aaacagatat
     2881 ggaagagccg atccaacagc caacggaaca ggcaagtgaa ccgaattcaa aagaaacagc
     2941 atctccaaag gaggcaacaa cgtcaaatat cgaagagctg gaatctgttt atacaaagga
     3001 cttacaaaca ttgataaata aaatccaagg cgttagtgaa gctgaagtca tggtcaattt
     3061 ggaatctaca agtattaaag tatatgagaa aaacttaatt aaagggcaac agactacaga
     3121 ggaaacggat acgaatgggg gaacgcgaga aattgaagat ggaacggaag aaacacaaac
     3181 agtgttagta aggcaaggtg aaaaggaagt ccctttactc attcaaacga aaaagccgga
     3241 agttagaggt gtatttgttg tagctaaggg agctgatcat gccacagtaa aaaaatggat
     3301 tgttgaagct gtttcccgtg tcttagatgt gccaacccat cgagtgtctg taatgccaaa
     3361 gaattagggg aggaatttaa atgttaaaaa aacaaacagt gtggctatta actatgttaa
     3421 gtttaatgat tttattaagc gtctattata tgacttcacc tgatagtgaa gatttagctt
     3481 atatcaatga tggacaagaa aatgcgcagg aagaaaaagc ggttcctact gattcagaag
     3541 ccaatgatga tgccgaagta gaggagattt ctaatgtagg gcaagatgaa ttgtttacga
     3601 caattcgtat ggagttacaa gataaacgta acatggaaaa agagagatta accgacatcg
     3661 ttgcttcaag ctccgctagt tctgctgaaa aagatgaagc tttacaagaa attgatgcaa
     3721 ttgaagactt ggcatcaaaa gaaaagattt tggaagaaac cattgtagct tctgccaact
     3781 acaaagatgt acttgttcga tctgaagatg atagtgtaac cgtacaagtg aaggttgaag
     3841 aaaatcttcc aaaatcagaa gctgtttcca ttattcaaat ggtcaaagat gaattaggag
     3901 ataaaaaagt aaatgtatcg tttctgcctt ctcaaggaga aacggaaaaa gatacagaat
     3961 agatggttga aatccggggg agcccggatt tttttatgct ataaaatgaa gggtcatagt
     4021 tatatgaaag ctacagtcac taaagtaccc acattttctt attactaatt gtgtcttcta
     4081 ttaataagga gcaaattcaa tataaaaacg ttctgttaag gtagttgcac tagtagtgag
     4141 tccatcctag ggagtaggaa tcgaactgac gcgcgctttt cattcgcttt ccattttcag
     4201 tttgttataa taaaaataca agctatttgt atgacatctc ttcataatat atcttgaagt
     4261 ttgtttatca tttatcgtaa aatattaaca ggagttatta gtacctaata ataagctaga
     4321 aaatgaaggg gtgccttgaa tatatgttaa atgtacaaga aatccaggaa ttaataaggt
     4381 taattgatca aacgtctatt gaagaattta agtatgaaac aagcggagct tctatttcat
     4441 taaaaaaatc ttctacaact ggacaagtac aacctaaaaa tatacctgaa aaaaatgtat
     4501 ctgaaacagt agcacagata gaatcagaaa aagcgagtgc agaaaataca caagatgcat
     4561 cttcccaaaa agaaagcaca gtagattatg atcatgaaat tgtttcacct atggtaggaa
     4621 cattttatgc agctccatcc ccagagagag aaaactttgt ttcaataggt agtaaagttg
     4681 ataagtcaag cgttgtttgt attgttgaag caatgaaatt atttaatgaa attgaagcag
     4741 aagttgaagg tgagattgtc gaaatattag tagaaaatgg tgagttggta gaatatggtc
     4801 agccgctatt tcgagttagt acaaagtaag gggatgtaaa gtgtgataaa aaaactgctg
     4861 atcgcaaata gaggggaaat tgctgttcga attattcgag cttgtaaaga gatgggtatt
     4921 gaaacagtag ctgtattctc ccaggctgat agtgaagctc ttcatgtgca aatggctgat
     4981 gaagcttatt gtattgggcc aactcaaagc aaggatagct acttaaattt taccaatatc
     5041 atgagtatag caacgctgac aggtgtagat gctattcatc caggctatgg ctttttagca
     5101 gagaatgccg atttcgctga aatatgccgt gcatgtaata ttacttttgt tggtcctacg
     5161 cctgaggcca ttcaaaaaat gggaataaaa gacgtagcta ggcaaacaat ggaagccgct
     5221 aatgttccta ttgttccagg ttcagatgga atcattcatg atgtagaaga agcaaaagaa
     5281 attgctaatc agatcggtta tccaatcatc attaaagcaa ctgctggcgg tggcgggaaa
     5341 ggaatacgag tagcgcgaac cgaggaagaa ttaacgaaag gggtagagat aactcaaaaa
     5401 gaagcagaga cttcttttgg taatgcgggt gtttatctcg aaaagtatat tgaggatttc
     5461 cgacatgtgg aaatccaagt tttagctgat aaacatggga atgttatcca tcttggtgaa
     5521 cgagattgtt ccattcaacg taggttgcaa aaacttgtag aagaagcgcc atctccagca
     5581 atcactccag aaatccggga aaaaatggga gaagcttccg tcaaagctgc aaaagctgta
     5641 ggctatgaag gcgctggaac tattgaatat atttttgatc gtaaaacaca tcaattttat
     5701 ttcatggaga tgaatacacg tatacaagtc gagcaccctg tgacagaatt aattactggt
     5761 attgatttaa taaaagaaca gatcaagatt gctgataacg aagtattagc atattctcag
     5821 tctgacattt cttttaacgg atgggctata gaatgtcgta ttaatgctga aaatcccttt
     5881 aagaatttta tgccttcagc tggaaaggtt gatatgtatt tgccacctgg aggtctaggg
     5941 gtccgagtag atgctgctgt ttacccagag tatcgaatac ctccttatta tgattcaatg
     6001 attgcgaaac tgataacgta cggtaaaact agagaagaag caattcatcg aatgaaaaga
     6061 gctttggatg agtttattgt tgaaggcgtg catacgacaa taccatttca ccataaaatt
     6121 atggagcatc ccgtttttat aaatggtgac tttaatacaa aatttcttga agaaaaccct
     6181 ataacgcaag acgaagatga ataaaggagt tgagtgtatg agtgaacaac cgttattaaa
     6241 cgtaagtgat gacagtttag gtaaggtcga gattgcgcca gaagtcattg aagttattac
     6301 tggtttagct gcttcagaaa tagacggact ttacgcaatg cgaggaaatt ttgcctctgg
     6361 cgtagctgag cggtttggta aaaaggcgca tagtaaaggg gtaaaagtag aattaacaga
     6421 gaacggaatt gtcattgatc tgtatgtcat tttacaattt ggtgtatcta ttccacatgt
     6481 ggcacagttg ttacaaacaa atattagaca aactttgaaa aatatgactg cgttagaaat
     6541 tgatgaaatc aatgttcacg ttgttggcat tcaaatggat tatccagaag aagcaacaga
     6601 aaaaacgaac agttaaatct tttttgtagc aataatttta ttaaaacgcc cttcatgaaa
     6661 aaatatgttc tcatgaaggg cgtttttctt ctgctatagg aaagtataaa atttaaaagg
     6721 tttatagtat aatttcgcca gaaaaacttg gcgatactcc aagatatttc taatcgtagt
     6781 gttccaatat ttggttcgta aaaaataaaa tctttaaaaa ataatatagt taagaagtga
     6841 cgtaatcctg tttttcgcaa gaagatggat taattacttt cagacaaatg tttgacaata
     6901 ttttagctct tccaatagag ttttgttgta tgattcattt tggatgtagc aattaaaggt
     6961 tgattgtagc gttgctattg gaatcgtgtt atgattttgg gagagatgaa ctaaaggagt
     7021 tagtaaggaa tgaatagaca tgaagctaga aagaaggcat tgcaaatcat attttctatt
     7081 gacattcatg aaatgcaatc cattcatgaa atggaacatt tggatgaaca agtaagaaag
     7141 gatgcctttt taaatctact tgtaaacgga gtaataaact ataaggtaga aatcgatcaa
     7201 atcattagtg agaaactaga gaattggagc ttagaaagga ttgcgtctgt tgaaaaagca
     7261 attttgcgga ttgcagtttt tgaaatacgc tacgtagaag atattcctgt aagtgtttct
     7321 attaatgaag ccattgagtt agcaaatacg tttggagatg aaaaatctgg taaatttatt
     7381 aatggtgtat tatcacgcat tagtgattaa tgatcaccaa gtattataaa cacccaaaat
     7441 taggtcagta tttgttatag taggaaaatg gttcagatga ttcgtaaatt tattcttgtt
     7501 tgatcagtaa taaagcggtt tttatgaaag tttaaattga agggggtact tgcattgtct
     7561 gcagaaatta ttaacgggaa aaatttgtca ttagctttaa aggaagaaat gaaaaaggaa
     7621 gtagatgcgt tgaagataga tggtattcat cctcaactca ctgttatcct tgttggagat
     7681 caccctgcat ctagatccta tgtgaatgga aaggaaaaag cttgtggtga aacgggaata
     7741 ggctcacata ttattgaatt accaaaaaca gttagcgaat cagatctttt gaagggaata
     7801 cataggctaa acgatgatgc aaatgtacat gggatacttg ttcagttacc attaccggtt
     7861 catatcaatg aacaaaaggt gattgaaact atatctcctg aaaaagatgt agatggcttt
     7921 catccaatta acattggtag aatgatgaca ggaatggata catatttgtc atgtacacca
     7981 tacggaatta taacgatgct tcgctcaaaa aatatttcta tagcaggaaa acacgcggtt
     8041 gttataggaa ggagtaatat tgttggaaag ccagtggcac aactgttgat aaacgaaaac
     8101 gccacagtaa ctacttgtca ttctaaaaca gaaaacctga agcaatttac acgaatcgct
     8161 gatatccttg ttgttgctgt aggaaaagcg aatattattg atgctagtta tgttaaagaa
     8221 ggggctaccg tgattgatgt agggatgaat cgtttagaga acggaaaatt aaccggtgat
     8281 gtagattttg actctgtaca agaaaaggca tcatttataa ctcctgttcc tcgtggagta
     8341 ggtcctatga cgatcactat gcttttaaag aacacgatta aagcagcaag aaggttggag
     8401 aaccgtgaag gataattatc taactgttac ggcattaact agatatatta aacgcaagat
     8461 ggatgtggat cctcatttac gccaagtatg gcttcgaggt gagatttcga actttaaaca
     8521 tcattctaga ggacatatgt atttaacaat taaagatgat cgatcacgaa ttcaagcagt
     8581 aatgtttgca ggcaataatc gctcgttaaa attccaacct gaaaatggaa tgaatgtttt
     8641 aattaaggga gaaattagtg tttttgaagc atatgggcaa tatcaactat atattcaaca
     8701 aatggaacca gacggcattg gcgcgctgta tgttgctttt gagcaattaa aagagaagtt
     8761 ggagaaacaa ggatactttg atcattatgt caaaaaaaat atcccgccat taccaaaaaa
     8821 tattggtatt attacgtcgc caacaggagc agctgtaagg gatattatta caaccattaa
     8881 acgacgctat cctattgttg agttaacggt cattccagca ttagttcaag gagaagcagc
     8941 tgctcgatcc attaaacagg ccattgaaat tgccaatcaa aagggagact ttgacctgtt
     9001 aattgttgga agaggcggcg gatcgattga agagctttgg agttttaatg aagaaatagt
     9061 ggcgaaagct atttttcatt ctaacattcc tatcatttct gcggttgggc acgagacgga
     9121 tacgacaata agtgattttg tagctgattt acgagcccca acccctacgg ctgctgcaga
     9181 aattgctgta ccgtcgagtc aagatcttaa agataagatt gctacactaa aacgcatggc
     9241 gacgaaagat atgcaagaaa aatggaaaca acagcaaaaa caccttttta gacttaaaca
     9301 gtcatatgct tttcgctacc cttatcagtt gctgaaacaa aaagaacaag aattagatgg
     9361 gaaaatagag caattacaaa aagcgatgtt acatacgaag gaagcaagta ataagtccta
     9421 cgagcactta tgggctcggt tcaccgttca acatcctgga aaacaagtag aaagaggaaa
     9481 acaacagctt caacaattag tgaaacaaca atatatggtg atgaaacaac tacatacaga
     9541 caaggtaaaa aaacttcata atgaaatgga taaattaagc ttgcttaatc cattagcaat
     9601 aatgaagagg ggattttcta ttccttatac aagtgcagga cagattatta agtctgtgca
     9661 tgctgttgaa cgggaggatg cgatttcttt acgtttagca gatggacttt taaagtgtaa
     9721 agttatagaa acaaaggagg agagtcaaaa tgactgagga agaattatca tttgaagaag
     9781 cgatgcaaca gttagaggaa gttgtagaaa agctggaaac aggagacgtt ccattagaaa
     9841 aggcaatcag ctactatcaa gatggcatga agctgtcgaa actttgtacg gataagttaa
     9901 accaagtaga agaaaaaatg accaaaatta tgaacgaaca gggagaatta gaaggattcc
     9961 cacttcagga ggaagaataa acgtggaacg aaaaatggag atgtatttag aggaaaaaaa
    10021 acaattcata gacagtgctt taaaaaagta tatgcaagct ttacaagttc ctcctgcatt
    10081 aaagcagtca atgacttatt cagtagaagc tggcggtaaa cgattaagac caatattatt
    10141 acttgcaagc tatgaagcgt ttcaaaagga tataactgta gctttaagtt ccgctgcagc
    10201 actggaaatg attcatactt attcattaat tcatgacgat ttacccgcaa tggatgatga
    10261 tgattatcgt agaggtaaac taaccaatca taaagctttt agtgaagcta cagctgtatt
    10321 agctggagat gctttgttga cttacagctt tgaaataata agtaaagatg acaaacttac
    10381 agctgcccaa aaagcagaat taatagcgct attagctgaa gcaagtggcc caaaagggat
    10441 ggtcgctgga cagattttag atatggaagc agaagatcga gcgattactt tacaagagct
    10501 tgaacgtgta catactttaa aaacaggtga gttacttcgc tacgctgtcc atgcaggagc
    10561 atacctagga ggggctacag agaagcaatt gcacgaatta caactttttg cttcttattt
    10621 aggacttatt tttcaagttc aagatgatat attggatgtt actggtgatg aagtaaagct
    10681 aggtaaaccg gtaggtagtg atgcgcttaa tttaaagagt acgtatccaa aattgcttgg
    10741 tataaatggt gctattgaga aaaaagaaca atatcttctt aaagcaaaag aatctctgca
    10801 agctgcaggc gtagaaaatt ctaatctgat ggatttagcc atttattttg gccaaagaga
    10861 ttattaaccc atagtttttt tctaaaatat atgatataat ttatgtagaa taaggaatgg
    10921 tgcagtattc tagtcagttc tccgcacctg aaggcgagcc taaaaattcg ccaaagggca
    10981 catcgatgaa gttccttgtg caggcttgag gcgcccagct ttgggtctgt gctgggagtt
    11041 aaggttatcg ggcgatccac aaaggcatgt gggcgttgac ccgatttccg cggaggccca
    11101 tctctgtagt aacagctaag cctagttgtc attatggaat ggggtatgaa cctgcagtag
    11161 acggtaaggg acacctgata tgcagcgtag cctgccttga gtggtgactg aggggattag
    11221 ggcaataaaa catgaaatta ggtggccact aattttatgt tgtaatctct atgaaatcag
    11281 cactgcaaaa gaggctaggg gacggttaag aactgctgag gaaatctcct agactgttca
    11341 ttcgacattt aaaagggatt atagtgtgga ctaagtggta atccagtcta gctcttggtg
    11401 acaaagctaa gatggtttta aaggggaacc gcctttgtgg caacacgggg taaccatttg
    11461 ggaaaaccta ctggacctaa gccgcagttt ttacctttta aatgaccatt cctgttatac
    11521 atagtataaa ataatgtaca agtattaaaa taatgaggta tgaaatgaaa tcacatgtaa
    11581 aacgatcgat taattttagt ttaactattg ccgttatcac ttttgtgtta gcggctattt
    11641 tttcagttat atcttcttca atattaagtg gtgttgcctg gtttatcgga ctttttattg
    11701 tatttgttat cgttttcgtt ggtgttgttt ttgatatgtt aggtatagca tcgacagcag
    11761 ctaatgaggc tccattccat gcaatggctt cagaaaaagt aactggggca aaagaagcag
    11821 tgattattat tagaaatgcc gatcgattcg ccagtttttg taatgatgtt attggtgata
    11881 tttctggaat tgtaagtgga actgcttcag caattgtagt actgcaaatg gctaatcttt
    11941 taggtcatgg cgagggttct tccgttcaaa ttatcatttc tgtagtgcta acaagtattg
    12001 tcgcagccct tacagttggt ggaaaagcaa tgggcaaatt ttttgccatt aatgcttcaa
    12061 caaaaatcat tttttttgca ggaaaagtca tttccattat ggaaaataaa cttaaaataa
    12121 gtgtactacc taaaaattaa taaaagggaa tttgcctgta tgagtaaaaa aagacttgat
    12181 gtgttattag tagaaaaatc acttgccgct acaagggaaa aagcaaagcg tttaattatg
    12241 gctggtctcg tgtttgctga tcaacaaaga ttagacaagc caggaatgca agtagatgaa
    12301 tcaataaatt tgacggtaaa aggaaaatta ttaccttatg ttggacgtgg aggtttaaag
    12361 ctcgaaaaag cgctaaagac tttttcactt acagtaacgg ataaaattgt aatggacata
    12421 ggttcatcta ctggtggctt tacagattgt gcattgcaga atggtgcatc cttatgttat
    12481 gccattgatg ttggttataa tcagcttgat tggaaactga gaaatgatga ccgagttatt
    12541 gtaatggaac gaacgaattt tcgttatgtt acggtgggca tgctggaaaa cggtcaacca
    12601 aattttgctt ctattgatgt ttcgtttatt tcactacgct tacttttgcc agtcttaaag
    12661 catttattgg aaaataacag tgatgttgta gcactagtta aaccacaatt tgaagcagga
    12721 aaagaacaag taggcaaaaa aggagttgtt cgagacagcc gtgtgcacca agctgttttg
    12781 agacagatta ttgactttgc agcgcaagaa aaatttcaga tattagattt aactttttct
    12841 cctataactg gaggagatgg aaatattgag tttttgttgc atttaggctg ggaaaaacat
    12901 aaaccagcga ctaaagattt ttctgatcac gaaattgaaa gcttagtctc tgaagcacat
    12961 gaagcattag ctcgaaaaaa acaaaacaat aagtaatctt aaaaatttta gcacagtgta
    13021 agagcatgtt tgctataatg cttaagattg catactttct tatcgtgtaa ttttaagaaa
    13081 tggaggcacc tgatgagcaa aatacaacga catataaaga taagggaatt aattacggat
    13141 aatgaaattg aaacgcaaga tgaacttgta gatcgattaa aaaacttagg ctataatgta
    13201 acacaagcaa cggtttccag agatattaaa gaattacact tggtaaaagt agcaacagca
    13261 aatggaacat ataaatacag tttgcctgca gaccaacgct ttaatccatt agataaatta
    13321 aagcgtttag tcatggacgc atttgttaaa attgattatg caagccattt tatcgtactc
    13381 aaaacactcc cgggaaatgc tcacgctgtt ggtgctttaa ttgaccagct ggattgggaa
    13441 gaaataatgg ggactatttg tggtgatgat acttgtttaa ttatatgcag aacagaggca
    13501 gacgctttaa cgataaaaga acgttttatc gagatgttgt aaaacgaaag tggggaatta
    13561 tgtcgtgctt acggaattgt ccattcagga ttttgcgata atcaacaagc tttccgttac
    13621 ttttaatgaa ggattaaccg ttttaacagg tgaaacggga gctggtaaat caattattat
    13681 tgatgcggtt caattgctcg ctggaggtag aggttctgtt gaatttgtcc gtcatggtgc
    13741 taaaaaggca gatattgaag gtttatttat tattgatcat gattcacatc ctgtgtatac
    13801 tatttgcgag cagtatggca ttgaaagtga agaccagcaa cttgtactta gacgtaccat
    13861 aacagcaaat gggaaaagta tttgtcgtgt taatagcaaa ttagtaactt tagctgtcct
    13921 acgcgaattt ggaaggacat taattgatat tcatagtcag cacgaaacac agtcccttat
    13981 gaatcctgag catcatttag atttgttgga tttataccag cctgaattaa ttaataaggc
    14041 gaaaaaagaa tacttgaaat tatatgacca attatctaaa ttaaaaaatc gttatcgtaa
    14101 attgagtgaa aatgaacaag aaacagctca acgattagat ttgttgcaat tccagttgcg
    14161 ggaattagaa gaagcccaat tacaacctaa tgaagacaag caattggaag aagaaaggaa
    14221 tactttagct aattttgagc gtatttattc atccttacaa cttgcttaca attctcttta
    14281 tggcgaacag aaagggcttg attgggtcaa tcaggcgcaa atagcgttgc aagataatag
    14341 taactatgac gcttccctag cacaaaaagc agaagatatt gcaaattatt actatgggtt
    14401 agaagaattg acctatgact taggaaatca aattgaaacg ttgcaatttg acccagaacg
    14461 cctaaatgaa attgaagctc gtttaaacga aattaatcgg ttaaaaagaa aatatggtcc
    14521 tactgtttcg gatatgttaa cttatatggc aaagatagaa gaagaagtag aagaaataag
    14581 caataaagat tctcatatat ccaaactcga acaacaaata aaagaaaaag ccaaggatgc
    14641 ttttttggaa gctcaagaat tgcgtgatat ccgaataaaa gctgcagagg ctttgacaaa
    14701 ggaaattcat gaagaattaa aagaccttta cttaggaaag acggaattta aggtcgattt
    14761 tgctaagaat atcgaaccac aaaaagaact agataagcat ttgcataaaa atggaatgga
    14821 tgatgtgaca tttttgatct ccacaaatcc aggtgaacct cctaaaccct taaataaagt
    14881 agcttctgga ggagagctat ctaggattat gttaagtttg aagaaaatat ttgcaagaca
    14941 tcaaggtgtt acaagtgtca tttttgatga agtagatact ggagtaagtg gtagagtggc
    15001 gcaagcgatt gctgaaaaga tatatcacat ctcacagaag tcacaagttt tatgcattac
    15061 acatcttccg caagtggcgg ctatggctga tacacataaa ttaattgaaa aagaagtaat
    15121 gaatcaacgg acatctacaa ctgtccatga attaagccaa aaattgcaaa tcaatgaact
    15181 tgctcgaatg attacaggaa caaagcttac agatacagcg gtggatcatg ctagagaaat
    15241 gttagaaatg gcgatccaat ttaaaggaaa agagcataat ttatagtaaa aagccagaaa
    15301 ataaactatc tggctttttc ttttgtcaaa aatgactaag aataaattcg ccaaattagg
    15361 ttgtgtcgta gttgagaagt taatatcaat gtgaaaaaga tgaaaaatta gttttgtttt
    15421 ctcaaaaaca atatacataa caaataaaaa agctgtaggt aatgttaggc aagcttgctt
    15481 atctttcacc agtttaaatc atcgacaaaa agcttaaatt aaaggtacag ataaaaggtg
    15541 gtttgggtat gaaaagagga gagtgaatga atttgcgaac aaagcacaac aaaatattac
    15601 gtttcggtat atgctttatg acaatgcttg ccttacttgc gcttcctgtt attacaagcc
    15661 tatttaaagc ggaaagtcga attacacaag cagctactac agcaactagc atgagtccag
    15721 aagatacttc tacacttcat caatttactg gattacctat tcaacaaaca aaagcacaat
    15781 caaaggaaga aataaaaatt atacccggtg gacaatccat tggggttcag ttacaaacca
    15841 agggtgtact cgttgttggt catcatttag taaagggaaa aacgggcaga gtttcacctg
    15901 gggaaaaagc agatattcaa gtcggcgatg ttattttaga aattgatgga aaagaagtta
    15961 aaaagatgaa agatgtaaaa ccgtttgtaa aacaagccgg acaatcaaaa aatgtgttgg
    16021 atataaaatt aaagcgagga aagaaaacaa tttctaccga attagagcca gctttggatg
    16081 aaaaagaaaa taattatcaa atcggattat atataagaga ctctgctgca ggaataggca
    16141 cgatgacctt ttatgatcca aattccaaga aatatggagc tttaggtcac gtcatttccg
    16201 acatggatac ccaaaagcct atagaaatta ataatggctc tatcgtacga tcttctgtga
    16261 cctccattga aaaaggaaat aatggtatac caggagaaaa acaagcaact ttttcagcag
    16321 ctgaagataa aattggatct attacaaaaa ataccgcatt tggtattttt ggaaagttaa
    16381 atcaaaattt agaggaacta aaaaaaccat tacctgtagc tctacctaac gaggtgaaag
    16441 aaggacctgc aaaaattttg acagtacttg ccggtgagga gataaaagaa tacgatgtcg
    16501 aagttgttag cagtgtacct cagaaaaatg cagcaacaaa aggaatggtt attcaaataa
    16561 aagataagga attgctcaat aaaacaggag gaattgtaca agggatgagt ggaagtccta
    16621 ttatacaaaa tggaaaaatt attggggcgg tcactcacgt atttgtaaat gatcctacat
    16681 cagggtatgg cgtccacatt aagtttatgc tagaggaagc tggtattaat acagctactt
    16741 catcaccaaa aaaagtaagt taaatgaaag gcttggttgg attttttcca attcaagtct
    16801 tttttacgat gtgagaaaag ttaaattgga caaagctaag aatttgaagt aattgtggta
    16861 tacttattta aagtaaaaaa agttttctct gagaggcata tgctgaaaag cgattgacga
    16921 tttcatgtac tgtttctata gcgcttttaa ccttatttct gtaaaatcta caaatattat
    16981 tcgacaaata tctatcttta tttatcgaat gtagtcgttt attttagaaa tattgagtaa
    17041 aataaagcaa atctcaaaaa aaccaatgtt ttttaaataa aaaaaggata atttacattt
    17101 atgtagaata tgtagaataa agagaagtaa tgtactacat aaaaggagga gcttttgtgg
    17161 aaaagattga aatttgttta gtagatgata atcgggaatt gattaagctt atggaagatt
    17221 attttgaaga acaatcggat attgaagtta ttggtacggc gtttaatgga agagattgct
    17281 tgaccatgct ggatgattta gaaccagatg tcttaattct agatatcatt atgccacatg
    17341 tggatgggct tgctgttcta aatacactac gttcttcgaa agagaatcct ccacacgtca
    17401 ttatgcttac agcttttggg caagaagaag tgatgaaaaa agcagtagat ctaggtgccg
    17461 cttattttat tttgaagcct tttgatttag ataacttagc tgagcaaata aggcaagtac
    17521 agggcagttc ctcttactct gttggtgttt caaaagttca gcgaaaagaa aggaaaaaac
    17581 aagatttaga agcaagtatc accaacatta ttcatgaaat aggagttcct gcgcatatta
    17641 aaggttatat gtatttacga gaagccatta cgatggtatt taatgatatt gagctgcttg
    17701 gttctatcac aaaggtttta tatccagata tagctaagaa atacaatact actgcttcac
    17761 gtgttgaacg tgccattcgt catgctattg aagtagcgtg gagtagaggg aatattgatt
    17821 ctatttctgc tttatttgga tatacaatca gcatttcaaa agcaaaacca acaaattcag
    17881 aattcattgc gatggtggct gaccatttac ggttagagca taaagcgagt tgaaaagttt
    17941 tcaaataaaa aacatatccc tcaaaaatat aagggatatg ttttttattt gaatgaaaaa
    18001 gattttaatt gacaaaaaaa gcattgtata atgattgaac agctttttct agatcgtcag
    18061 ctataatgcc aaacatcata gaaacttctg atgacccctg attaatcatt tcaatattta
    18121 cattcgcact agaaatggcg gacgttgctt tttgagcgac gccaattgta cgcacaagtc
    18181 cttctcccac aatcataatc atagcgagat tacgatgaat agagattaaa tcagggcata
    18241 acaccttttg aatacgtttt aaaacttgct tttctttttc taggggtaaa tgtttttccc
    18301 gaataataat tgataaatca tcaatacctg aaggagcatg ttcaaaggaa ataccttcct
    18361 cttctaaaat agagagcact ttacgtccaa aaccaatttc tctgttcatc aagtatttac
    18421 taatatagat actacaaaaa ccagtgtcac tggcaatgcc aacaacacat ttttcgcttg
    18481 gttttttctc agaaacaatg aatgtgccag ttgcttgcgg attatttgta tttttaatgc
    18541 atactggtat tttttcacga tatgcaggaa ttaatgcttc atcatgaaac acggaaaatc
    18601 ctgcataaga caattcacgc atctctttat acgtaagtgt tgttattttt ttagggttgt
    18661 ctacgatgga aggattaact gcgtaaacag agtcaacgtc tgtataattt tcatatagat
    18721 ccgctttaac tccagcggct ataatggatc cggtaatatc agaaccacca cgtgaaaatg
    18781 taattaattt tccagcattt gtatatccaa aaaaaccagg aataactgaa ataccttttg
    18841 tagagcgtaa attatatatc atctcaaaac tttctggcaa tatttttcca ttacctggtt
    18901 catcactgac cattatacca gcttcgcgag ggtttacgta acgtgcttct aaaccaattg
    18961 aagtaagata agcgctaaga attttagctg aactatcctc accaattgct tttaaagcat
    19021 ctaatttgct aatgggtgct tcctccatat gtaatagctt attaatggac tctttaattt
    19081 cccccacaat aatgaaagac aagcctaact gttctacaat ttcagtaaat cttttaatga
    19141 ttggatcaat atatggctct aaagaagttt cctgttggct tgctttccat aattgaatga
    19201 acatgtccgt cattttttgg tctgatttat gttgtttacc tggagcagaa acgacaataa
    19261 attttttttg gaggtccttc tttataatgt tggcaacttt ctttatttgt tcggcactag
    19321 ctacagagct ccctccaaat tttgcaactt tcacgtgtga attcctcccg ctgatttttt
    19381 atttttcatc atatcataaa ttctgtttat tttcacatta taaagaataa aaatgaatgt
    19441 acttacttta agtgaaggga agcatgtaga aggtaatgtt gaatgtctac aattaaaaaa
    19501 atggagactt agtccccatt tgtataacaa ttcctttttt gttgctgatt tttttgtcgt
    19561 tttcgatcac ggtgtataat gaaaccacct atgaaagcaa gtccagccaa gaatagtata
    19621 agcccaataa tgaactgaat actactatgt aaaaatattg gatagaaagt agcaaataat
    19681 gtgtctctca ttaacttaat gccaatagca gctattaaac ccgggataaa cataataagt
    19741 atggctatga ttcggatcat tgaaaattct ccttcattat tgtcaagtat agtatagcaa
    19801 aaaaggatag aaaaaggaat aaaggaaaac accacttcaa gattgactat agaaataaat
    19861 gaaatacaca tattgcatta ctttgcagac taacgtaaga taaaagtatg caaaaaaatg
    19921 caaggtggga tggaatgaaa cgcgttctta ttgttggtgc tggtaaaggt gggagtgcaa
    19981 tattaaaaat tctccatgct actcaaacta tgaaagtcgt agctatcgtg gacacgaata
    20041 cgtctgctcc cggtctagta gcggcagaaa aatgggacat tcctataggg cgttcgtgga
    20101 aaaaatggat tgatgaaaat attgatatta tcattgaagc aactggtaat gaatttgtat
    20161 tagaagcact attacagcga cgtaatcgaa ggacagtaat tattccaggg acagttgctt
    20221 atatcatttc tgagctattt gatgaaaaac aagagctgtt agaagaaata gaactacaaa
    20281 tgctaaatca agagttgata ttaaacaata tacgtgatgg catgattgtc gtcgatacta
    20341 atgggtttat cacttttgtt aataaaagtg cagaaaagat tattaatacc tcaaagcaca
    20401 gactacttga aggagaacat atagaaaaag tcatttatcg tacacgtctt actgaagtgt
    20461 taaaaaaccg tagaaaggaa attaatcaaa aattaacttt agaaaacggg aaacagatta
    20521 ttgcaacaag gattccaatt atacggtcag acaatagtct actaggtgcg tttgctgttt
    20581 ttaaagatat tacggaagtc gtacatttag cagaagaaaa cacagactta aaagagatta
    20641 aaacgatgct agaagcaatt attcattctt ctgatgaagc aatcagtgtt gtagacgaac
    20701 aaggtcttgg gttaatgata aatcctgcct atactaaaat cactggatta gctgcctctg
    20761 atgtaattgg caaaccagca actacggata tttctgaggg agagagtgta catatgaggg
    20821 tcttacaaac gagaagaccc ataagaggag tattaatgaa agttggc
//
