LOCUS       NZ_FTMW01000001        20747 bp    DNA     linear   CON 22-JUN-2024
DEFINITION  Bacillus subtilis strain RU36A, whole genome shotgun sequence.
ACCESSION   NZ_FTMW01000001
VERSION     NZ_FTMW01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2017) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900156365.1-RS_2024_06_22
            Annotation Date                   :: 06/22/2024 02:00:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,232
            CDSs (total)                      :: 4,119
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 7, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 1 (5S, 16S)
            partial rRNAs                     :: 6, 10 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 89 of 145
            Pseudo Genes (incomplete)         :: 67 of 145
            Pseudo Genes (internal stop)      :: 30 of 145
            Pseudo Genes (multiple problems)  :: 33 of 145
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:06:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 615769
            Orig. end                         :: 636516
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FTMW01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20747
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="CDPS"
                     /region_number="4"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..20747
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20747
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="False"
                     /core_location="[10000:10747](+)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10747
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     gene            1021..3894
                     /gene="uvrA"
                     /locus_tag="BW968_RS03250"
                     /old_locus_tag="SAMN05878487_0644"
     CDS             1021..3894
                     /EC_number="3.1.25.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="uvrA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391396.1"
                     /locus_tag="BW968_RS03250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0644"
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="WP_003228057.1"
                     /transl_table=11
                     /translation="MAMDRIEVKGARAHNLKNIDVTIPRDQLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMDKPDVDAIEGLSPAISIDQKTTSRNPRSTVGTVT
                     EIYDYLRLLYARVGKPHCPEHGIEITSQTIEQMVDRILEYPERTKLQVLAPIVSGRKGA
                     HVKVLEQIRKQGYVRVRIDGEMAELSDDIELEKNKKHSIEVVIDRIVVKEGVAARLSDS
                     LETALRLGEGRVMIDVIGEEELMFSEHHACPHCGFSIGELEPRLFSFNSPFGACPTCDG
                     LGMKLEVDADLVIPNQDLSLKENAVAPWTPISSQYYPQLLEAVCTHYGIDMDVPVKDLP
                     KHQLDKVLYGSGDDLIYFRYENDFGQIREGEIQFEGVLRNIERRYKETGSDFIREQMEQ
                     YMSQKSCPTCKGYRLKKEALAVLIDGRHIGKITELSVADALAFFKDLTLSEKDMQIANL
                     ILREIVERLSFLDKVGLDYLTLSRAAGTLSGGEAQRIRLATQIGSRLSGVLYILDEPSI
                     GLHQRDNDRLISALKNMRDLGNTLIVVEHDEDTMMAADYLIDIGPGAGIHGGQVISAGT
                     PEEVMEDPNSLTGSYLSGKKFIPLPPERRKPDGRYIEIKGASENNLKKVNAKFPLGTFT
                     AVTGVSGSGKSTLVNEILHKALAQKLHKAKAKPGSHKEIKGLDHLDKVIDIDQAPIGRT
                     PRSNPATYTGVFDDIRDVFAQTNEAKVRGYKKGRFSFNVKGGRCEACRGDGIIKIEMHF
                     LPDVYVPCEVCHGKRYNRETLEVTYKGKSISDVLDMTVEDALSFFENIPKIKRKLQTLY
                     DVGLGYITLGQPATTLSGGEAQRVKLASELHKRSTGRTLYILDEPTTGLHVDDIARLLV
                     VLQRLVDNGDTVLVIEHNLDIIKTADYIVDLGPEGGAGGGTIVASGTPEEITEVEESYT
                     GRYLKPVIERDKTRMKSLLKAKETATS"
     gene            4233..5147
                     /gene="hag"
                     /locus_tag="BW968_RS03255"
                     /old_locus_tag="SAMN05878487_0645"
     CDS             4233..5147
                     /codon_start=1
                     /gene="hag"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391416.1"
                     /locus_tag="BW968_RS03255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0645"
                     /product="flagellin Hag"
                     /protein_id="WP_031315352.1"
                     /transl_table=11
                     /translation="MRINHNIAALNTLNRLSSNNSASSKNMEKLSSGLRINRAGDDAAG
                     LAISEKMRGQIRGLEMASKNSQDGISLIQTAEGALTETHSILQRVRELVVQAGNTGTQD
                     GTDLQAIQDEISSLVEEIGGGTNANGISDRTEFNGKKLLNGAFSEDSGKSLTFQIGANG
                     SQQLSVNIESMAATAIGALDDDGNLVADQTVAKIDVTKFADNAADTATVGFDAQLKTVD
                     DAINTVSKQRSKLGAVQNRLEHTINNLSASGENLTAAESRIRDVDMAKEMSDFTKNNIL
                     SQASQAMLAQANQQPQNVLQLLR"
     gene            complement(5346..5576)
                     /locus_tag="BW968_RS03260"
                     /old_locus_tag="SAMN05878487_0646"
     CDS             complement(5346..5576)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391394.1"
                     /locus_tag="BW968_RS03260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0646"
                     /product="hypothetical protein"
                     /protein_id="WP_003228059.1"
                     /transl_table=11
                     /translation="MEKDPSDYTVTQESVLKLIQEQKRMNREMITELEQIHGPFPISHD
                     IQYIKVLLDSSNTHIVQDLMSVSKQLYKKTL"
     misc_feature    5545..5560
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.68"
                     /tool="antismash"
     gene            5794..6120
                     /gene="yvlA"
                     /locus_tag="BW968_RS03265"
                     /old_locus_tag="SAMN05878487_0647"
     CDS             5794..6120
                     /codon_start=1
                     /gene="yvlA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391393.1"
                     /locus_tag="BW968_RS03265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0647"
                     /product="protein YvlA"
                     /protein_id="WP_003244375.1"
                     /transl_table=11
                     /translation="MNRNQAIIASLCYFSVFIAPIIVPIVAYFVVNEKETKRHAIRSLI
                     SHIVPFVGWLFLFIALLGGAVAIDGDSLLPVFVIIGGAVIYFLVVIGIIIWNVIQGIKV
                     LRAA"
     gene            6145..7242
                     /gene="yvlB"
                     /locus_tag="BW968_RS03270"
                     /old_locus_tag="SAMN05878487_0648"
     CDS             6145..7242
                     /codon_start=1
                     /gene="yvlB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391392.1"
                     /locus_tag="BW968_RS03270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0648"
                     /product="DUF4097 domain-containing protein"
                     /protein_id="WP_014478112.1"
                     /transl_table=11
                     /translation="MKQEKERILKLVEEGKLTAQEALTLIEKLDSDYKEKEEKITALSV
                     HVHDEEEPFTTAKKESGKPSLGAKLFDWIDSAVKKVKEVDLDLNFGHAYDVQHIFQFKD
                     TDFSSVELQIANGSVNIVPWEDDDIRAECQAKVYRADSQDAARHAFLQHIECEIKGNKF
                     FIRTEKKTMKTNVTLYIPQKEYDKIRVKLFNGPVRGEHLHVKEFSAKTTNGVLSFSHLT
                     AEKAIAETANGQIKLASHSCGTIEAETINGLIDLRGKSESIDVQSFNGNIAINVTESDC
                     RSIYAKTTTGSVELAIPDDLAVKAELKSNLGSLSHELMDVEMLKEKNDTIQKEMMFTSN
                     QAHDQNITVFSESLTGAIKLKYSQR"
     gene            7247..7444
                     /gene="yvlC"
                     /locus_tag="BW968_RS03275"
                     /old_locus_tag="SAMN05878487_0649"
     CDS             7247..7444
                     /codon_start=1
                     /gene="yvlC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391391.1"
                     /locus_tag="BW968_RS03275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0649"
                     /product="PspC domain-containing protein"
                     /protein_id="WP_003228065.1"
                     /transl_table=11
                     /translation="MNKLYRSEKNKKIAGVIGGLAEYFNWDASLLRVITVILAIMTSVL
                     PVLLIYIIWIFIVPSERDMK"
     gene            7446..7805
                     /gene="yvlD"
                     /locus_tag="BW968_RS03280"
                     /old_locus_tag="SAMN05878487_0650"
     CDS             7446..7805
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene="yvlD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391390.1"
                     /locus_tag="BW968_RS03280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0650"
                     /product="phage holin family protein"
                     /protein_id="WP_003228066.1"
                     /transl_table=11
                     /translation="MVKWAVSILVNALLLIVIDGYIDSIHISSIGAAIIASLILSILNV
                     LIKPLLIIFTLPVTMVTLGLFLFVINAITLMMTASIMGDSFQIDGFGTAIWASVILSVF
                     HLLIQKGILEPLRKK"
     gene            complement(7834..9045)
                     /gene="pchE"
                     /locus_tag="BW968_RS03285"
                     /old_locus_tag="SAMN05878487_0651"
     CDS             complement(7834..9045)
                     /codon_start=1
                     /gene="pchE"
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391389.1"
                     /locus_tag="BW968_RS03285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0651"
                     /product="MFS transporter"
                     /protein_id="WP_003228068.1"
                     /transl_table=11
                     /translation="MAHTKSKAVLILYTVCFSAFFASLSQNIYSPILPIIKESFHVSTA
                     MVNLSVSVFMIVTAIMQIILGAIIDFKGARIVLITGILATAAASIGCAVTTDFTLFLIF
                     RMIQAAGSAALPLIAATTIGQLFTGNERGSAMGTYQMLLSVAPAIAPVLGGFIGGAAGY
                     EGIFWILAAISIVLLVTNSITFPKDSPTESMQQAKGNVFAHYKSIFTNRTGNVILTLSF
                     VLFFIYFAVIVYLPILLTEHYHIDVGIAGLLYLPLALSTIAGTFLFKRIQAKIGLHTLF
                     IGSNVIAACSIILFAVTHSVSLVLMALTLALFGISMGVIPPLYSTMITNEFEHNRGSAI
                     GMFNFIRYTGMAAGPMVSAYLLTMMPSAMSFSLLGLGFAALSFCLLPPMFSPQKRTKQK
                     KHHM"
     gene            complement(9066..9575)
                     /gene="pchR"
                     /locus_tag="BW968_RS03290"
                     /old_locus_tag="SAMN05878487_0652"
     CDS             complement(9066..9575)
                     /codon_start=1
                     /gene="pchR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391388.1"
                     /locus_tag="BW968_RS03290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0652"
                     /product="pulcherriminic acid biosynthesis transcriptional
                     regulator PchR"
                     /protein_id="WP_029318331.1"
                     /transl_table=11
                     /translation="MSDLTKQMIYDIYVRLLHLNEQKANTSLQQFFKEAAEEDVIDIPK
                     NMTSIHVIDCIGQHEPINNAGIARKMNLSKANVTKISTKLIKEEFINSYQLTDNKKEVY
                     FKLTRKGRRIFDLHEKLHKKKELAFYQFLDSFSQEEQKAVLKFLEQLTSTLEAEQTDGT
                     PDKPVK"
     gene            10001..10747
                     /gene="pchC"
                     /locus_tag="BW968_RS03295"
                     /old_locus_tag="SAMN05878487_0653"
     CDS             10001..10747
                     /EC_number="2.3.2.22"
                     /codon_start=1
                     /gene="pchC"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391387.1"
                     /locus_tag="BW968_RS03295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0653"
                     /product="cyclo(L-leucyl-L-leucyl) synthase"
                     /protein_id="WP_003242712.1"
                     /sec_met_domain="CDPS (E-value: 3.1e-83, bitscore: 274.8,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGMVTERRSVHFIAEALTENCREIFERRRHVLVGISPFNSRFSE
                     DYIYRLIGWAKAQFKSVSVLLAGHEAANLLEALGTPRGKAERKVRKEVSRNRRFAERAL
                     VAHGGDPKAIHTFSDFIDNKAYQLLRQEVEHAFFEQPHFRHACLDMSREAIIGRARGVS
                     LMMEEVSEDMLNLAVEYVIAELPFFIGAPDILEVEETLLAYHRPWKLGEKISNHEFSIC
                     MRPNQGYLIVQEMAQMLSEKRITSEG"
     gene            10763..11980
                     /gene="cypX"
                     /locus_tag="BW968_RS03300"
                     /old_locus_tag="SAMN05878487_0654"
     CDS             10763..11980
                     /EC_number="1.14.15.13"
                     /codon_start=1
                     /gene="cypX"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391386.1"
                     /locus_tag="BW968_RS03300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0654"
                     /product="pulcherriminic acid synthase"
                     /protein_id="WP_017695292.1"
                     /sec_met_domain="p450 (E-value: 1.6e-36, bitscore: 122.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQSIKLFSVLSDQFQNNPYAYFSQLREEDPVHYEESIDSYFISR
                     YHDVRYILQHPDIFTTKSLVERAEPVMRGPVLAQMHGKEHSAKRRIVVRSFIGDALDHL
                     SPLIKQNAENLLAPYLERGKIDLVNDFGKTFAVCVTMDMLGLDKRDHEKISEWHSGVAD
                     FITSISQSPEARAHSLWCSEQLSQYLMPVIKERRVNPGSDLISILCTSEYEGMALSDKD
                     ILALILNVLLAATEPADKTLALMIYHLLNNPEQMNDVLADRSLVPRAIAETLRYKPPVQ
                     LIPRQLSQDTVVGGMEIKKDTIVFCMIGAANRDPEAFEQPDVFNIHREDLGIKSAFSGA
                     ARHLAFGSGIHNCVGAAFAKNEIEIVANIVLDKMRNIRLEEDFCYAESGLYTRGPVSLL
                     VAFDGA"
     gene            complement(12021..12494)
                     /gene="pchA"
                     /locus_tag="BW968_RS03305"
                     /old_locus_tag="SAMN05878487_0655"
     CDS             complement(12021..12494)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pchA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391385.1"
                     /locus_tag="BW968_RS03305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0655"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_003228083.1"
                     /transl_table=11
                     /translation="MDNSIHDELFQAIQQFALKRDKRVWQKVQIPSIGISSQHHDHLKK
                     DWTLTQLHIVSCIHTSQNVNNSFLASRLHISKAAVSKAVHALLKHNIITVTKKPGNKKE
                     IFYTLTDSGRKLAALHEQLHEKAKEQYKQLFNEFSIDDLKTVTAFFNLWIKYM"
     gene            12659..16528
                     /gene="yvnB"
                     /locus_tag="BW968_RS03310"
                     /old_locus_tag="SAMN05878487_0656"
     CDS             12659..16528
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="yvnB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391384.1"
                     /locus_tag="BW968_RS03310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0656"
                     /product="metallophosphoesterase"
                     /protein_id="WP_076457528.1"
                     /transl_table=11
                     /translation="MRKYTVIASILMSFLSVLSGGHHESKAVPVVQQELFQTPHYIPLL
                     ENPPQIDVKAEMRSNLMIEAQVRDNSYQAFSAFLFYKQSNELGYKMVPMDPTPGAVRKF
                     LAQIPKRLIWNSELEYYIVMSNGKQRVESETKKLKLEGYQADLAHIPELLITELAVDTK
                     NIGRADGYEFIEIFNTTDRTIDFKDYHIRYRYPKEGPDSDLIWRPDERILIPSGETFVV
                     WLKPAGHPELTSEDFNRYYQTQLKEGKNLAVIDETEGMANTRPRAVVISTNTGKDISVA
                     HYRKHALRRLSSVLYKYPLNGTAELLNISIGEKNPSPGTVLQAQVPEQKRKIKLDKEKP
                     VIEDLTDRKPVRPAESIELRADIRDRSLVKTAAFYYRTDENKPFKRILAEKDRNDNLFH
                     YIVYSPELIGKDQLEYYVAAGDGINEARTPVKMIDIKQTSKAHGLRMNIENRDTLSGTQ
                     FLKATTEGRADSIKLWIDGKKQVTEPAMEKEVYFAFDTRKTNLYFKNAVTMEGKVLKVF
                     DDTTNKYRTYSVPLPETLLRKGKQLQRITIRSGSKVSPFDTAENRDDFLVKNARLVLSD
                     GTVIRDQRVSPEKELFIGDNQRSNKSWQFQFNLPDGLFTSQLLEWDTSKLSEGAHHIQA
                     SDGKENVSLVVRVDNSGPHIEPNITEGQTYKGNLILQADMYDKWSRIEEAEASLDGESI
                     TLPYHTSSSDLLPGKHSLKVTATDLAGNKTVIERIFKTEREHPDQPEVIDSEADTHKAK
                     ISVRVKDPTNDAMDVGFYRGFQYTARNHVKIFKHASLTEPPKSFVPERETPFTNKELER
                     VSAADGKTVSTENKELFPYHRFEVTVDPSIDENDLAEAVWKGSSLPGRKVTMYAWNYRT
                     NEWQPVDSFVAKDDKPFTLKASVIAADFVRESKMNVIVQDEIPPAKDMYTFVWMSDTQY
                     YAESYPHIFDKQTEWIKDNQKQLNIKYVFHTGDIVDDSADIRQWKNADRSMSVLDKSGI
                     PYGVLAGNHDVGHKDGSYRAFGKYFGSDRFDKKLHYGGSYKNNRGHYDLISSNGNDYIM
                     LYMGWGITDEDIAWMNQVLKKHPDRMAILAFHEYLLVSGNRSPIGEKIFKEIVKPNPNV
                     VMVLSGHYHSAMRKTDELDDDGDGKPDRLVHQMLADYQGGPEGGQGYLRLLQFDQANDM
                     VHVSTYSPYVKDKNYYDTDTYGNKDEFSLSLDLKPRIKKVETDYFECNVYTNEELGKRE
                     QVKSGDTAEFRWDHLEPQSVYYWYIIVEDSFNGKTKSPIWKFKTKKETYRPAPDQFDFR
                     HVSNP"
     gene            complement(16548..17279)
                     /gene="nagR"
                     /gene_synonym="yvoA"
                     /locus_tag="BW968_RS03315"
                     /old_locus_tag="SAMN05878487_0657"
     CDS             complement(16548..17279)
                     /codon_start=1
                     /gene="nagR"
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /gene_synonym="yvoA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391383.1"
                     /locus_tag="BW968_RS03315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0657"
                     /product="N-acetylglucosamine utilization transcriptional
                     regulator NagR"
                     /protein_id="WP_014478108.1"
                     /transl_table=11
                     /translation="MNINKQSPIPIYYQIMEQLKTQIKSGELQPDMPLPSEREYAEQFG
                     ISRMTVRQALSNLVNEGLLYRLKGRGTFVSKPKMEQALQGLTSFTEDMKSRGMTPGSRL
                     IDYQLIDSTEELAAILGCGHPSSIHKITRVRLANDIPMAIESSHIPFELAGELNESHFQ
                     SSIYDHIERYNSIPISRAKQELEPSAATTEEANILGIQKGAPVLLIKRTTYLQNGTAFE
                     HAKSVYRGDRYTFVHYMDRLS"
     gene            complement(17297..18025)
                     /gene="nagB"
                     /locus_tag="BW968_RS03320"
                     /old_locus_tag="SAMN05878487_0658"
     CDS             complement(17297..18025)
                     /EC_number="3.5.99.6"
                     /GO_function="GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA]"
                     /GO_process="GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nagB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391382.1"
                     /locus_tag="BW968_RS03320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0658"
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="WP_029318335.1"
                     /transl_table=11
                     /translation="MKVMECQTYEELSQKAARITADTIKEKPDAVLGLATGGTPEGTYR
                     ELIRLHQTENLSFQNVTTVNLDEYARLSSNDPNSYHFYMNDRLFQHIDSKPSRHFIPNG
                     NADDLEDECRRYEQLVESLGGTDIQLLGIGRNGHIGFNEPGTSFKSRTHVVTLNEQTRQ
                     ANARYFPSIDSVPKKALTMGIQTILSSKRILLLISGKSKAEAVRKLLEGNISEDFPASA
                     LHLHSDVTVLIDREAASLRP"
     gene            complement(18022..19212)
                     /gene="nagA"
                     /locus_tag="BW968_RS03325"
                     /old_locus_tag="SAMN05878487_0659"
     CDS             complement(18022..19212)
                     /EC_number="3.5.1.25"
                     /GO_function="GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA]"
                     /GO_process="GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nagA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391381.1"
                     /locus_tag="BW968_RS03325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0659"
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="WP_076457529.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.5e-42, bitscore:
                     142.1, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAESLLIKDIAIVTENEVIKNGYVGINDGKISTVSTERPKESYSK
                     EIQAPANSVLLPGMIDIHIHGGYGADTMDASFSTLDIMSSRLPEEGTTSFLATTITQEH
                     GNISQALVNAREWKAAEESSLLGAELLGIHLEGPFVSPKRAGAQPKEWIRPSDVALFKK
                     WQQEAGGLIKIVTLAPEEDQHFKLIRHLKDESIIASMGHTDADSALLSDAAKAGASHMT
                     HLYNAMSPFHHREPGVIGTALAHDGFVTELIADGIHSHPLAAKLAFLAKGSSKLILITD
                     SMRAKGLKDGVYEFGGQSVTVRGRTALLSDGTLAGSILKMNEGARHMREFTNCSWTDIA
                     NITSANAAKQLGIFDRKGSVTVGKDADLVIVSSDCEVILTICRGNIAFISKEADQI"
     misc_feature    complement(19267..19282)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
     gene            19394..20326
                     /gene="hprK"
                     /locus_tag="BW968_RS03330"
                     /old_locus_tag="SAMN05878487_0660"
     CDS             19394..20326
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hprK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391380.1"
                     /locus_tag="BW968_RS03330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05878487_0660"
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="WP_003228097.1"
                     /transl_table=11
                     /translation="MAKVRTKDVMEQFNLELISGEEGINRPITMSDLSRPGIEIAGYFT
                     YYPRERVQLLGKTELSFFEQLPEEEKKQRMDSLCTDVTPAIILSRDMPIPQELIDASEK
                     NGVPVLRSPLKTTRLSSRLTNFLESRLAPTTAIHGVLVDIYGVGVLITGKSGVGKSETA
                     LELVKRGHRLVADDCVEIRQEDQDTLVGNAPELIEHLLEIRGLGIINVMTLFGAGAVRS
                     NKRITIVMNLELWEQGKQYDRLGLEEETMKIIDTEITKLTIPVRPGRNLAVIIEVAAMN
                     FRLKRMGLNAAEQFTNKLADVIEDGEQEE"
ORIGIN
        1 ttcttgatta tttcccagac gattttatga tcgtggtgga tgagtcgcat gtgacaattc
       61 cccaggtgcg cggcatgttt aacggagacc aggcgcggaa acaggtgctc gtggatcacg
      121 gcttccggct tccatcagcg cttgataacc gtccgcttcg ttttgaggaa tttgaaaagc
      181 atatgcacaa tatcgtgtat gtatccgcaa caccagggcc gtacgagatt gaacatacag
      241 atgaaatggt cgaacagatc attcgtccga cggggcttct tgacccgctt attgacgtcc
      301 gtccaatcga aggtcagatt gacgacttga tcggcgaaat tcaagcaaga atcgagcgaa
      361 atgaacgggt gcttgtgaca actttgacga agaagatgtc agaggatctg accgattatt
      421 taaaggaaat cggcattaaa gtaaactatc tgcattctga aatcaagacg cttgaacgga
      481 ttgaaattat ccgcgacctg cgccttggca agtacgatgt gcttgtcggc atcaacctgc
      541 tgagggaagg tttggacatt cccgaagtat cccttgttgc gattttggat gcggataagg
      601 aaggtttcct tcgttcggag cggtcgctga tccagaccat cggacgggca gcgagaaatg
      661 cggaaggccg cgtcattatg tacgccgata aaataacgaa gtctatggaa attgcgatta
      721 atgaaacaaa acgccgacgc gagcagcagg agcggtttaa tgaagagcac ggcattacgc
      781 cgaaaacgat aaataaagaa attcgcgatg tcattcgtgc cacagtagca gctgaggata
      841 aagcggaata caaaacaaaa gccgcaccga agctgtcgaa aatgacgaag aaagaacgcc
      901 agaaagtcgt tgaacagatg gagcatgaaa tgaaggaagc cgccaaggcg cttgactttg
      961 agagagccgc ggagcttcgc gatttacttt tagagctaaa agcggaagga tgatgaacaa
     1021 atggctatgg atcggataga ggtgaaggga gccagggcgc ataacctgaa aaatatagat
     1081 gtaacgattc cgagagatca gcttgtcgtt gtcacgggtt tgtccggatc aggtaaatcc
     1141 tcccttgcct ttgacacgat atatgctgaa ggacagagac ggtatgtcga gtcgctgtct
     1201 gcttatgccc gccagttttt agggcaaatg gataagcctg atgtggatgc aattgagggg
     1261 ctctctcccg ccatcagcat tgatcagaaa acaacgagcc gcaatccgag gtctactgtc
     1321 ggtacggtaa ctgagattta tgattatctg cgtcttttat atgcgagagt agggaagcct
     1381 cattgtccgg agcacggaat tgagattaca tcccagacca tcgagcaaat ggtggacaga
     1441 attctggaat acccggaacg gacgaagctt caggtgctgg cgccgattgt ctcgggccga
     1501 aaaggcgctc atgtcaaagt gcttgaacag attagaaaac aaggctatgt cagagtcaga
     1561 attgacggcg agatggctga gctttccgac gatatcgaat tagaaaagaa caagaagcat
     1621 tccattgagg tagtcattga ccggattgtc gtgaaagaag gcgtggcagc ccggctgtca
     1681 gattcattgg aaacggcgct ccgtttaggt gaaggacggg ttatgatcga tgtcatcggt
     1741 gaggaagagc tgatgttcag cgagcatcat gcctgtccgc actgcggatt ttcaattggt
     1801 gagcttgagc cgcgtctgtt ttcgtttaac agtccgttcg gggcgtgtcc gacgtgtgac
     1861 ggtctcggaa tgaagcttga agtggatgcc gatcttgtca tccccaatca agatttgtca
     1921 ttgaaggaga atgcggtcgc cccttggaca ccgatcagct cacaatatta tcctcagctg
     1981 cttgaggcag tctgcaccca ctatgggatt gatatggatg tgccggtcaa agatttgccg
     2041 aagcatcaac tggataaagt gctgtacggc agcggagatg acctgattta tttccgatat
     2101 gaaaatgatt ttggacaaat ccgtgaaggt gaaattcaat ttgaaggcgt attgcgcaac
     2161 attgaaagac gctataagga gacaggctct gatttcatcc gtgagcaaat ggagcagtat
     2221 atgtctcaga agtcttgtcc gacgtgcaaa ggctatcggt taaagaaaga ggcgcttgcc
     2281 gtactgattg acggccgcca cattggaaaa attaccgagc tgtctgtcgc cgacgcactt
     2341 gccttcttta aagaccttac cctttctgag aaggatatgc agatcgccaa tttgattttg
     2401 cgcgaaattg tggagcgctt aagctttctg gacaaagtcg gcctcgatta cctgacattg
     2461 agcagggcag cgggtacatt gtccggagga gaggcgcagc gcatcaggct ggcgactcaa
     2521 attggctcgc gcttatccgg tgtgctttat attttagatg agccgtctat cggtctgcat
     2581 cagcgtgata acgatcgctt gatcagcgct ctgaaaaata tgagagacct cgggaacacg
     2641 ctgattgttg tcgaacatga tgaggacacg atgatggcag cagattattt aatagacatt
     2701 ggaccgggag ccggcattca cggcggacag gtgatatctg cgggtacgcc ggaagaagtg
     2761 atggaagatc caaactcatt aacgggcagc tatttatcag ggaaaaagtt tatcccattg
     2821 cctcctgaaa gaagaaagcc ggacggacgt tacattgaaa ttaaaggtgc ttcagaaaac
     2881 aacctgaaaa aagtgaatgc caagttcccg cttgggacgt ttacagcagt tacaggtgtt
     2941 tccggttcag gaaagagtac actcgttaat gaaattttgc ataaggcgct ggcgcaaaag
     3001 cttcataaag cgaaagcgaa gcccggcagc cataaagaga ttaaaggttt ggatcattta
     3061 gacaaagtca ttgacattga ccaggcgcca atcggaagaa cgccgagatc caaccctgcg
     3121 acatacaccg gtgtatttga tgacattcgt gatgtattcg cgcagacaaa tgaagcgaag
     3181 gtccgcggct ataaaaaagg ccgtttcagc ttcaacgtga agggcggacg atgtgaagcc
     3241 tgccgcggag acgggattat taaaattgaa atgcacttcc ttcctgacgt atacgttcca
     3301 tgcgaggtgt gtcacggcaa acgctataac cgtgaaacgc ttgaagtgac gtacaaagga
     3361 aaaagcatct ctgatgtgct tgatatgacg gttgaagatg ctctttcttt ctttgaaaat
     3421 atcccgaaaa tcaaacgcaa gctccaaacc ctttatgatg ttggtttagg ttatattacg
     3481 ctcggccagc cggcgacgac cttgtcaggc ggagaagcgc agcgcgtgaa gctcgcgtca
     3541 gagctgcaca aacgctcaac cggacgcacg ctctacattt tagatgagcc gacgacaggt
     3601 ttgcatgtcg acgatatcgc caggcttctt gtcgtgctgc aacggttggt agacaacgga
     3661 gacactgtac tggttattga gcacaacctt gatatcatta agacagccga ttacattgtg
     3721 gatctgggcc cggaaggcgg agccgggggc ggaaccattg tagcgtcagg aacgcctgag
     3781 gaaatcactg aagtggaaga atcgtataca ggccgttatt tgaagcctgt tatcgaacgt
     3841 gacaaaacac gcatgaaatc gctcttaaaa gcgaaagaaa cagctacatc ttaaaaccct
     3901 ctgttaagag gggacagctt gtcagcaagt ccatccttgg gcttagcagg caagcttttt
     3961 ctttacggca taataggttt agaatcgtct ttgggtaaaa tttctttggg aaaacgccta
     4021 aacatttaca aattgctgtc gatatatgaa ttgaaaggcg gatgatgatt tccaaattaa
     4081 gcagaaaaac cctattaagg ggcttaaagg catccttaat caataactga atttacatag
     4141 gttagaagct ttattggaat ctgatacgcc agaaatcaaa gaaaaggaag tctttgattt
     4201 caaaaacata tacacatgga ggtatttttc acatgagaat caaccacaat attgcagcgc
     4261 ttaacacact gaaccgtttg tcttcaaaca acagtgcgag ctcaaaaaac atggagaaac
     4321 tttcttctgg tcttcgcatc aaccgtgcgg gagatgacgc agcgggtctt gcgatttctg
     4381 aaaaaatgag aggacaaatc agaggtcttg aaatggcttc taaaaactct caagacggaa
     4441 tttctcttat ccaaacagct gagggtgcat tgactgaaac tcactcaatt cttcaacgtg
     4501 tccgtgagct agttgttcaa gcaggaaaca caggtactca agatggtact gaccttcaag
     4561 ccatccaaga cgagatatct tcacttgttg aagaaatcgg gggtggcaca aatgctaacg
     4621 gtatttctga ccgtactgaa ttcaacggta agaaactcct taacggtgct ttctcagaag
     4681 atagtggtaa atcactaaca ttccaaattg gtgccaacgg tagccaacag ctttctgtca
     4741 acattgaaag tatggccgca acagcaatcg gtgctctaga tgatgatggt aatcttgttg
     4801 ctgatcaaac agtagctaaa atcgatgtaa ccaaatttgc tgataatgca gctgacactg
     4861 caactgtagg attcgatgct caacttaaaa cagttgatga cgctatcaac actgtttcta
     4921 aacaacgttc aaaattaggt gcggtacaaa accgtctaga gcacacaatc aacaacttaa
     4981 gcgcttctgg tgaaaacctg acagctgctg agtctcgcat ccgtgacgtt gacatggcga
     5041 aagaaatgag cgatttcaca aagaacaaca ttctttctca agcttctcaa gcgatgcttg
     5101 ctcaagcaaa ccaacagccg caaaacgtgc ttcaattgtt acgttaattt tgaacggcta
     5161 tctctttctg atagttacag cttgccgaca gagtccatta actggatgtg ccggcaggct
     5221 ttttttgtta caatcatttt gtgacagcca gggaatcact tgttttttca aaacaaaaaa
     5281 ggcaagatat tgtcatctaa cctttagctt tgtttcagtt ataatgaatg atatgttttc
     5341 cgcaattaca aagtcttctt atacagctgc ttacttacac tcattaaatc ttgaacaata
     5401 tgtgtgttgc tgctgtcgag cagcactttt atatattgga tatcgtgaga gatcgggaag
     5461 ggtccgtgaa tctgctcaag ctcggtgatc atttcacgat tcattctttt ttgttcctga
     5521 atcagcttta acactgattc ttgtgttact gtatagtcac tgggatcttt ctccattgct
     5581 tgtccagcct ccttggttgc cgtaagcttt ttacatattg taatcggtat agccggaaga
     5641 ttctacttct aaaacttttt aaaatccaat ttccgtaaat cggagcatgt ctaaagaatg
     5701 aatttgaaac ctgaagagat tttaaacgta taaataagta aaacgtccct gatcttttgt
     5761 catggacaat tcacaatagg agtgtggaat gaattgaacc gtaatcaagc tattattgca
     5821 tcactttgtt atttcagtgt gttcattgcg ccaatcattg tgccgattgt cgcatatttt
     5881 gttgtaaatg agaaagaaac aaaacgccat gcgatacgct ctcttatttc tcacattgtt
     5941 ccgtttgtcg gctggctgtt cctttttatt gctttacttg gcggagccgt tgcgattgat
     6001 ggagacagct tgcttcctgt atttgtaatc atcgggggag cggttattta tttcttagtt
     6061 gtcattggca ttatcatttg gaatgtgatt cagggcatta aggtcctgcg tgcagcataa
     6121 gtagacaatg gggaggagat cgtaatgaag caagaaaagg aacgaatcct taagcttgtt
     6181 gaggaaggaa agcttacagc acaagaagct ttgacactga ttgaaaagct tgacagcgac
     6241 tacaaggaaa aagaagagaa aatcacagcg ctctcagttc acgttcatga cgaggaagag
     6301 ccatttacaa ctgcaaaaaa agaaagcggc aagccgtctt taggagccaa gctgtttgac
     6361 tggattgatt ctgctgtgaa aaaggtcaag gaagttgacc ttgatttaaa ctttggccat
     6421 gcgtatgatg ttcagcacat ttttcaattt aaagatactg acttttccag tgttgagctg
     6481 caaattgcca atggcagcgt gaatatcgtc ccttgggaag atgacgatat cagagcggaa
     6541 tgccaggcga aggtatatcg cgctgacagc caggatgcgg cgagacacgc atttcttcag
     6601 catattgaat gtgaaatcaa aggaaataaa ttttttatcc gtactgagaa aaagacgatg
     6661 aaaacgaatg tcactttgta tattcctcag aaggagtatg ataagattcg agtgaagctg
     6721 tttaacggtc cggtcagagg ggaacattta catgtaaaag agttctcagc gaaaacgaca
     6781 aacggcgtgc tgtcgttctc gcacttaaca gccgaaaaag ccatagctga aacggccaac
     6841 gggcaaatta agctcgccag ccacagctgc ggaacgatag aagcggaaac cattaacgga
     6901 ttgatcgatc tccgagggaa aagcgaatcc attgacgttc aaagctttaa cggaaatatt
     6961 gcgattaacg taacagaatc agactgccgc tccatttatg caaaaacaac gactggaagt
     7021 gtggagcttg cgattccgga cgatctcgca gtgaaggcag agctgaaaag caatcttggt
     7081 tcgctgtccc atgaattaat ggatgttgag atgctgaagg aaaaaaatga taccattcaa
     7141 aaagaaatga tgttcacttc aaatcaggca catgatcaaa acatcaccgt cttctcagaa
     7201 tcactaacag gcgccatcaa actaaagtac tcacagaggt gatcgaatga ataagcttta
     7261 tcgctcagaa aaaaataaga aaatcgccgg tgttatcgga ggtctcgcgg aatatttcaa
     7321 ttgggacgca tcactcctca gagtcataac tgtcatctta gccattatga caagtgtctt
     7381 gccggttttg ctcatttata tcatttggat ttttatcgtc ccgtcagaaa gggatatgaa
     7441 atgacatggt aaaatgggca gtcagcattt tagtcaatgc attgttatta atcgtcattg
     7501 atggatatat tgactccatc catatcagca gcattggggc agcgattatc gccagcctca
     7561 ttttatcgat cttaaacgtg ctgattaaac cgcttttaat tatctttaca ctgccggtca
     7621 ccatggtgac gcttgggctc ttcttgtttg tgattaacgc catcacgctt atgatgaccg
     7681 cgtccattat gggtgacagc tttcaaatcg acggcttcgg cacagcgata tgggcatccg
     7741 ttatcctgtc ggtcttccat ttgctgattc aaaaaggcat tttagagccg cttagaaaaa
     7801 aataaaaaaa agctgcccgc aaaggcagct tttttacatg tggtgctttt tttgtttcgt
     7861 gcgcttctgc ggcgaaaaca ttggcggaag aaggcaaaag ctcaatgcgg caaatccaag
     7921 gcctaggagg ctaaaggaca tggcagacgg catcattgtg agcaagtagg cagataccat
     7981 cggacctgct gccatgcctg tatatcggat aaagttaaac attccgattg cactccctct
     8041 gttgtgctca aattcattag taatcattgt agagtacaag ggaggaataa cccccatcga
     8101 gatgccaaac agtgccagcg tcagagccat gagaacgaga gaaacggaat gtgtaacagc
     8161 aaataaaatg atgctgcagg cggcaatcac attgcttccg ataaacaagg tgtgcagccc
     8221 gatttttgcc tgtattcttt taaacagaaa cgtacctgca atcgtgctca gcgccagcgg
     8281 caaatataac agtcctgcta tacccacatc tatatggtaa tgctctgtca gcaatattgg
     8341 gaggtagaca attactgcaa aataaatgaa aaagagaaca aaacttaaag tcaaaatgac
     8401 gttccctgtt cgatttgtaa atattgattt ataatgagcg aacacattgc ctttggcttg
     8461 ctgcatagat tcagttggag aatctttagg aaaggtgatg ctgtttgtca ccagcaaaac
     8521 gatagagatg gccgcaagta tccaaaaaat cccttcgtat ccggctgctc cgcctatgaa
     8581 tcctcctaga actggggcaa tagccggtgc gacagacagg agcatttgat acgttcccat
     8641 tgcactcccg cgttcattgc ctgtaaacag ctgtccgatc gttgtggcag caataagagg
     8701 cagtgctgcg gaaccggctg cctgtatcat tctgaatatc agaaacaaag taaagtcagt
     8761 agtcaccgca cagccgatgc tggctgctgc cgttgccaga ataccggtaa tcaagacgat
     8821 ccgagcgcct ttaaaatcaa tgatcgctcc taatataatt tgcattattg ctgtcacaat
     8881 cataaaaact gagactgaca ggttcaccat agctgtggaa acatggaatg attctttaat
     8941 gatcggaaga ataggtgaat aaatgttctg gcttaaagat gcaaaaaatg cactgaagca
     9001 aacagtgtat aagatcaata ctgcctttga ttttgtatga gccaaagtgt acaccgcctt
     9061 tttgtttact ttacaggttt gtccggagtc ccatcggttt gttctgcttc aagtgttgac
     9121 gtcaactgct ctagaaactt caatacagcc ttttgttctt cttgtgaaaa tgaatcgagg
     9181 aattggtaaa aagccagctc ctttttttta tgcagtttct catgtaagtc gaaaatccgt
     9241 ctgcctttac gggttaattt aaaataaact tcttttttgt tatctgtcag ctgatagctg
     9301 ttaatgaatt cttccttgat cagttttgtg ctgattttcg ttacattcgc tttcgataag
     9361 ttcatttttc tggcaattcc ggcattatta atgggttcat gttggccgat acagtcaatg
     9421 acatgtatgc ttgtcatgtt tttagggata tcaatcacat cctcttctgc ggcttcctta
     9481 aaaaattgct gaagtgaagt attcgctttt tgttcattaa ggtgcagcag tctcacgtat
     9541 atgtcgtata tcatctgttt tgtcaaatca gacataggct accttctttc ttagtaataa
     9601 tagtttacga tgaaacaata aaacaaatcg tttactggtg aacaatttca ttttatcttt
     9661 tcttgttttc gttgtcaatt gttggtgttt agactatttg ttgatctgtc atgctgaata
     9721 tgcgtgtaaa aagtgtgtgg aaaatgtgat gtttacattt attttcaaat atttgggcac
     9781 agatcatatt gacgtttgga aggaaaaggg ataaaatgac taatgtttac tagtaaacat
     9841 ttatcatgat atgtgaaatg gaaatagtta ttaaaatttt aaaaaacatt tggaaacttt
     9901 aagtgctgcc tgccattcgt cttgttcagt tgttctagct attccatctt gtatttgagg
     9961 gtgatgactt aaagcgggat aagttttagg ggtgaatgag atgaccggaa tggtaacgga
    10021 aagaaggtct gtgcatttta ttgctgaggc attaacagaa aactgcagag aaatatttga
    10081 acggcgcagg catgttttgg tggggatcag cccatttaac agcaggtttt cagaggatta
    10141 tatttacaga ttaattggat gggcgaaagc tcaatttaaa agcgtttcag ttttacttgc
    10201 agggcatgag gcggctaatc ttctagaagc gcttggaact ccgagaggaa aggctgaacg
    10261 aaaagtaagg aaagaggtat cacgaaacag gagatttgca gaaagagccc ttgtggctca
    10321 tggcggggat ccgaaggcga ttcatacatt ttctgatttt atagataaca aagcctacca
    10381 gctgttgaga caagaagttg aacatgcatt ttttgagcag cctcattttc gacatgcttg
    10441 tttggacatg tctcgtgaag cgataatcgg gcgtgcgcgg ggcgtcagtt tgatgatgga
    10501 agaagtcagt gaggatatgc tgaatttggc tgtggaatat gtcatagctg agctgccgtt
    10561 ttttatcgga gctccggata ttttagaggt ggaagagaca ctccttgctt atcatcgtcc
    10621 gtggaagctg ggtgagaaga tcagtaacca tgaattttct atttgtatgc ggccgaatca
    10681 agggtatctc attgtacagg aaatggcgca gatgctttct gagaaacgga tcacatctga
    10741 aggatgatag ggggagtaaa acatgagcca atcgattaaa ttgtttagtg tgctttctga
    10801 tcaatttcaa aacaatccat atgcttattt ttcacaactg cgggaggaag atccggttca
    10861 ttatgaagag tcgatagaca gttattttat cagccgctat catgatgtcc gctatatcct
    10921 tcagcatccg gatatcttca cgacgaaatc acttgttgag cgtgccgaac cagtcatgcg
    10981 aggccctgtg ctggcccaaa tgcatggaaa agaacactct gccaaaagaa gaattgtagt
    11041 gagaagcttt atcggtgacg cactggatca tctgtctcca ttgattaaac aaaatgcaga
    11101 aaacttgtta gcgccttatc ttgaaagagg gaaaattgat ctcgtcaatg attttggaaa
    11161 gacgtttgcg gtgtgcgtca cgatggacat gctcgggctg gataaaagag accatgaaaa
    11221 aatctctgag tggcacagcg gagttgccga ttttatcacg agtatctctc aatctcctga
    11281 agcgcgggca cattcgttat ggtgcagcga acagctttcc caatacttga tgccggtcat
    11341 taaagaacgt cgcgtcaatc cgggatcaga tttaatttcg atcctatgta cttctgaata
    11401 tgaaggcatg gcgctgtcgg acaaggatat actcgcactg attcttaatg tgctgttagc
    11461 cgcaacggaa ccggctgata agacgctggc actgatgatc taccatttgc tcaacaatcc
    11521 tgagcagatg aatgatgttt tggctgaccg ttcgttagtt ccgagagcca ttgcggagac
    11581 attgcgttat aaaccgccgg ttcagctgat tccgcggcag ctgtcccaag atacagtggt
    11641 cggcggtatg gaaatcaaaa aagatacgat tgttttttgt atgatcggtg cggctaaccg
    11701 ggaccctgaa gcatttgaac agcctgacgt gtttaatatt catcgggaag atcttggtat
    11761 caagagcgct tttagcggcg ccgcccggca tctcgctttc ggatccggca ttcataactg
    11821 tgtaggagca gcttttgcca aaaacgaaat cgaaattgta gctaatattg tgctggataa
    11881 gatgcggaat atcagattag aggaagattt ttgttatgct gagtccggtc tgtatacacg
    11941 cggacctgtt tcacttctcg ttgcgtttga cggggcataa tagaattcca aaggtctctc
    12001 ccatgcgggt gagactcttt ttacatatat ttgatccaca agttgaaaaa cgcagtcact
    12061 gtttttaaat catcaattga aaattcattg aaaagctgtt tatattgctc ctttgctttc
    12121 tcatgaagct gttcatgtaa tgccgccaat tttctgccgc tatcagttag cgtataaaaa
    12181 atttcttttt tgttgccagg cttttttgtc actgtaatga tattgtgttt caacaaagca
    12241 tgaaccgctt ttgaaacagc ggcttttgag atgtgtaaac gggatgctaa gaagctgttg
    12301 ttcacatttt ggctggtatg tatgcaggaa acaatgtgaa gctgggtcaa agtccagtct
    12361 tttttgaggt gatcgtgatg ttgtgaggat atgccaatgg atggtatctg caccttttgc
    12421 cagacgcgtt tatccctttt taatgcgaat tgttgtatag cttgaaataa ttcatcatga
    12481 atgctgttgt ccatcaattt gcctctctga tttaattgtt aactggttaa ctatccttag
    12541 tttactgggt aatagtggaa ttttcaagag acagacgttt ttttgttctt tatgcacaaa
    12601 aatgaactca taagcttccc tgttcagacg caaaatacat gaaaagggag gtgctttgat
    12661 gaggaaatac acggttattg cttctatatt aatgtctttt ctttccgttt tgtctggcgg
    12721 ccatcatgaa tcgaaagctg ttcctgttgt acagcaggaa ctatttcaaa cgcctcatta
    12781 catacccttg cttgaaaatc ctcctcaaat tgatgtgaaa gctgaaatga gaagcaatct
    12841 aatgattgag gctcaagttc gcgataattc gtatcaagca ttttccgcct ttctttttta
    12901 taaacaaagt aatgagctgg gctacaaaat ggtgccgatg gacccgaccc ccggagcggt
    12961 ccgcaagttt ttggcacaaa ttccgaaacg tttgatttgg aacagcgagc ttgagtatta
    13021 cattgtgatg tcgaatggga agcagcgggt ggagtcggag acgaagaaac tgaagcttga
    13081 aggataccag gctgaccttg cccatattcc ggagcttctg atcacagagc tggcggttga
    13141 tacgaagaat ataggacgtg cagacggtta tgaatttatt gagattttta atacgactga
    13201 cagaacgatt gattttaaag actaccatat ccgttaccgc tacccaaaag aagggcctga
    13261 ctccgatctg atttggagac ctgatgaaag gatcttgata ccatccggcg aaacgtttgt
    13321 cgtctggctg aagcctgccg ggcatccaga attgacgtca gaggatttca atcgttatta
    13381 tcagacacaa ctaaaagaag gaaaaaatct cgcggtgata gacgagacag agggtatggc
    13441 caatacgagg ccgcgggccg ttgtgatatc aacgaataca ggaaaggaca tttcggttgc
    13501 gcattatcgg aaacatgcgc tgcgccggct gtcatcagtg ctttataagt atccgttgaa
    13561 cggaaccgca gaacttctga acatttcaat cggggaaaag aatccgtctc ccggaaccgt
    13621 attacaggca caggtacccg agcagaaacg gaaaatcaag ctcgataaag aaaagccggt
    13681 cattgaggat ttgactgacc gcaagcctgt ccgccctgct gaaagcattg agctgcgggc
    13741 tgacatacgg gaccgaagcc tagtgaaaac agcggccttt tactaccgga cggatgagaa
    13801 caaaccgttt aagcgaattc ttgctgaaaa agaccgcaac gataatctct tccattatat
    13861 tgtgtattcg cctgaattga tcgggaagga tcagctagag tattatgtgg cggccgggga
    13921 cgggatcaat gaagcgagaa cgccggtcaa aatgattgat attaaacaaa caagcaaggc
    13981 gcacggtctg cggatgaata ttgaaaacag agacacgctg tcgggaactc aatttctcaa
    14041 agccacgaca gaagggcgag ctgacagcat taagctgtgg attgacggga aaaaacaagt
    14101 gacagagccc gcgatggaaa aagaagtata ttttgcattc gatacgagga aaacgaacct
    14161 ttattttaaa aacgcggtaa cgatggaagg caaagtgctg aaggtgttcg atgatacaac
    14221 aaataaatac cgcacctatt ctgttccatt gccagagaca ctgcttcgga aagggaagca
    14281 gctgcagcgt attacgatac gttcaggttc gaaggtgtct ccgtttgata cggctgaaaa
    14341 ccgtgatgac ttcttagtca aaaacgcccg attggtgtta tcggatggaa cggtgatcag
    14401 agatcagagg gtgtctcctg agaaggaact ctttattggc gataatcagc gctcaaataa
    14461 aagctggcaa tttcagttca atctgcctga cggactattt acatcacagc tgctggagtg
    14521 ggatacatca aagctttctg aaggcgctca ccatattcaa gcaagcgacg ggaaggaaaa
    14581 tgtcagcctg gttgtacggg tagataattc aggacctcac attgagccga atatcaccga
    14641 ggggcaaacg tataaaggaa accttatcct gcaggcggat atgtatgata aatggagcag
    14701 aattgaggaa gcggaggcgt cgttagatgg agaaagcatt acactcccat atcatacgtc
    14761 atcttctgac cttttgccgg gaaaacattc tctaaaagtg acagcaacgg atctcgcggg
    14821 caataaaaca gtgattgaga ggatatttaa aaccgaacga gagcatccgg accagccgga
    14881 ggttatagac agcgaagccg acacgcataa ggccaagatt tctgtacggg tgaaagatcc
    14941 gacgaatgat gccatggatg ttggttttta taggggattt caatatacag cgcgcaatca
    15001 tgtgaaaata tttaagcacg cgtcactgac tgaaccgccg aaaagctttg tgccggaaag
    15061 ggagacccca tttacgaata aagaattgga gcgggtatca gctgctgatg gaaaaacagt
    15121 atcgacagaa aacaaagagc ttttccctta tcatcgcttt gaagtgacgg ttgatccatc
    15181 aattgatgag aatgatctgg cagaagcagt ctggaagggc agttctttgc cggggagaaa
    15241 ggtgacgatg tatgcttgga attatcggac aaacgaatgg cagcctgttg acagcttcgt
    15301 cgcgaaggat gacaaaccgt ttacgctgaa agcgtctgtg attgccgccg attttgtacg
    15361 ggagtcgaaa atgaatgtga ttgtacagga tgaaattcct cctgcgaagg atatgtacac
    15421 atttgtctgg atgtctgata cacagtatta cgccgaaagc tatccgcata tttttgataa
    15481 acagaccgaa tggataaaag ataatcaaaa gcagctcaat atcaaatatg tgttccacac
    15541 gggtgatatt gtagatgatt ccgccgatat cagacagtgg aaaaacgctg atcggtcaat
    15601 gtccgttctg gataagagcg gaattcctta cggcgtgctg gccggcaatc acgatgtcgg
    15661 acataaggac ggctcctaca gggcgtttgg caaatatttt ggcagcgatc gttttgataa
    15721 aaaattgcat tatggcggtt cctataaaaa taaccgcgga cattatgacc tgatttctag
    15781 caacggcaat gattatatca tgctgtacat gggatggggc attacggatg aagacatcgc
    15841 gtggatgaat caggtgctga agaaacaccc tgaccggatg gccattttag cttttcatga
    15901 gtatttgctg gtcagcggaa atagaagccc gatcggagag aaaatattca aagaaatcgt
    15961 aaagccgaac ccgaatgtcg tgatggtatt gagcgggcat tatcatagtg cgatgagaaa
    16021 aacagatgag ctggatgatg atggtgatgg gaagccggat cgtcttgtgc atcaaatgct
    16081 ggccgattat cagggcgggc cagaaggcgg tcaagggtat ttgcgtttgc ttcaatttga
    16141 ccaggccaat gacatggtgc acgtatcgac gtattctccg tatgtaaagg ataagaatta
    16201 ctatgatacg gatacctatg gcaacaagga tgaattttcc ctttcgcttg atttgaagcc
    16261 aagaatcaaa aaggtggaaa ccgattattt tgaatgtaat gtatatacca atgaagagct
    16321 cggaaaacgg gagcaagtga agagcgggga tacagctgaa ttccgctggg atcaccttga
    16381 accgcagtct gtttattatt ggtacattat agtggaagac agttttaacg gcaaaacgaa
    16441 atcaccgatc tggaagttca agacgaaaaa agaaacatat cgtccggcac cagaccagtt
    16501 tgattttcgc catgtctcca atccttaaaa agggttggag gcttttttta tgaaagacga
    16561 tccatatagt ggacaaatgt ataacggtcg cctctgtata cggattttgc atgctcaaaa
    16621 gcagttccgt tctgcagata tgttgttcgt ttaattaata ggactggcgc tcccttttga
    16681 ataccaagaa tgttcgcttc ttccgtggtg gcagcgcttg gctcaagctc ctgttttgca
    16741 cgggaaatcg gtatgctgtt gtacctttca atatgatcat agatcgacga ctgaaaatgt
    16801 gattcgttca attcacccgc aagctcaaac ggaatatgtg aggactcaat cgccatcgga
    16861 atatcatttg ccagccgcac ccgagtgatt ttatggatag aggaggggtg cccgcagcct
    16921 aatatagccg cgagttcctc agtcgaatca ataagctgat aatcaatgag cctgctgccg
    16981 ggtgtcatcc cgcggctttt catatcctcg gtaaagcttg tcagcccttg aagtgcttgt
    17041 tccatttttg gcttgctgac aaaggtgccc cgccccttca ggcgatagag caagccttca
    17101 ttaactaaat tagaaagcgc ctggcgaact gtcatccggc tgatcccgaa ttgttcggca
    17161 tattcgcgct cagaaggaag aggcatatcc ggctgcagtt ctccgctctt aatttgggtt
    17221 tttaattgct ccataatctg ataataaatc ggaataggcg attgtttatt gatattcata
    17281 agtcagcatg ttcctttcaa ggtcttaatg acgcggcttc acgatcaatc aaaaccgtga
    17341 catcggaatg caggtgcaaa gcagatgcgg gaaaatcttc gctgatgttt ccttctaaca
    17401 gcttacgaac agcttccgct ttgctttttc cagaaataag cagcagaatg cgcttgcttg
    17461 agagtatcgt ttgaattccc attgtcaatg ctttttttgg cacgctgtct atggagggaa
    17521 aatatctggc atttgcctgg cgagtttgtt catttaaagt cacaacatga gttcgcgact
    17581 tgaaagacgt tcccggttcg ttaaagccga tgtgtccgtt tcggccgatg ccgagaagct
    17641 gaatgtcagt gccgccgagg gactcgacta gctgttcata ccgtctgcat tcgtcctcca
    17701 aatcgtctgc atttccattc ggaataaaat gccggctagg cttgctgtcg atatgctgaa
    17761 ataaacggtc attcatatag aagtgatagc tgttcgggtc attgcttgaa agtctggcgt
    17821 actcatccaa attaactgta gtgacgtttt gaaatgagag attctcagtt tggtgcaggc
    17881 ggatcagctc gcgatacgtg ccttccggtg tgccgcccgt cgctaacccg agaacggcat
    17941 caggtttttc tttgattgta tctgccgtta ttctggctgc tttttggctt agctcttcat
    18001 acgtttgaca ttccattact ttcatatctg gtcagcctcc ttggatataa atgcaatgtt
    18061 tccgcggcaa atggtgagaa tcacctcgca atcactgctg acaatcacca aatctgcatc
    18121 ttttcccaca gtcacactgc cttttcggtc aaagatgccc agctgtttgg ccgcgtttgc
    18181 agaagttata ttagctatat ccgtccaaga gcaatttgta aactcacgca tatgccgtgc
    18241 gccttcgttc attttgagga tcgaaccggc aagggttccg tctgaaagaa gagctgttcg
    18301 tcctcttacc gtcacacttt ggccgccgaa ctcatatacg ccgtctttca gtccttttgc
    18361 cctcatggaa tcggtaatta aaatgagctt gctgctgcct ttcgccaaaa aagcaagctt
    18421 tgcagctaaa ggatgggagt gaatgccatc ggcgataagc tctgtcacaa acccatcatg
    18481 ggccagcgcc gttccgatca ctccgggctc tcggtgatga aacgggctca tcgcgttgta
    18541 cagatgagtc atatggctcg cccctgcttt tgcggcatcc gacagcaatg cggaatcggc
    18601 atccgtgtgc cccatggatg cgataatcga ttcgtctttc aaatggcgga tcagtttaaa
    18661 atgctgatcc tcctccggcg caagtgtaac aattttgatc agtccgcccg cctcctgctg
    18721 ccactttttg aaaagcgcaa catccgaagg cctaatccac tcctttggct gcgctccggc
    18781 tcttttaggc gaaacaaagg ggccctctaa atgaatgccg agcagttctg cacctaataa
    18841 agaggactct tcagccgctt tccactctct tgcgttcacc aaagcttgag aaatgttccc
    18901 atgctcttgg gtaatcgttg tggctaaaaa gctcgtcgtg ccttcttcgg gcagtcttga
    18961 cgacatgatg tcgagcgtgg aaaagctagc atccattgta tctgcgccat agccgccgtg
    19021 gatatgaatg tcaatcatgc cgggaagcaa aacggaattc gcaggtgctt gaatttcttt
    19081 tgaatatgac tccttgggcc gttctgttga gactgtgctg atttttccgt cattgattcc
    19141 gacatagccg tttttgatta cttcattttc tgtcacgatc gcaatgtctt tgataagaag
    19201 actctctgcc atagatgatc cgcctttctg gaatttgaat acctttattt tactcttttt
    19261 cagactagtg atctagacca gctgaggtgt tttcccgcct tttataaaat ctgttttagg
    19321 ttcaagcctg aaagtgataa aatgaaagta ttgtgagcaa gctcggggca gcccggaaca
    19381 ggaggaacat atcgtggcaa aggttcgcac aaaagacgta atggaacagt tcaatttgga
    19441 attaatcagc ggagaagaag gaattaaccg cccgattaca atgagtgacc tatcaagacc
    19501 aggcattgaa attgccggat attttacata ttacccgaga gagcgcgtgc agcttctcgg
    19561 aaaaacagag ctttctttct ttgaacagct tcctgaagag gagaaaaaac agcgaatgga
    19621 ttctctgtgc actgatgtaa caccagctat tattctttct agagatatgc ccatcccgca
    19681 ggagctgatt gacgcctccg agaaaaatgg ggtgcctgtc ctcagatcgc cgctgaaaac
    19741 gacaagactg tcaagccgtt taaccaactt ccttgagagc aggctggcgc ctaccacagc
    19801 aattcatggc gttcttgtcg atatatatgg tgtcggcgtg ctgatcacag gaaagagcgg
    19861 cgtcggaaaa agcgaaacag cgctggagct tgtgaaaaga gggcatcgcc ttgtcgccga
    19921 tgattgcgtt gaaattcggc aggaggatca ggatactctt gtcggaaacg ctccggaatt
    19981 aattgagcac cttcttgaga tcaggggcct tggcattatc aatgtgatga cgctatttgg
    20041 cgctggcgcg gtcagaagca ataaacgaat cacgattgtc atgaatcttg aactctggga
    20101 gcagggcaag caatacgacc ggctcggact tgaggaagag accatgaaaa tcattgatac
    20161 agaaattaca aaactgacga ttcccgtccg cccaggcaga aatcttgcag tcattatcga
    20221 agtggcagca atgaacttca gattgaagcg gatggggctg aatgccgctg aacaatttac
    20281 caacaagctg gcggacgtca ttgaagacgg tgaacaagaa gaataggagt tggcgttaaa
    20341 tgaatgaagc gatagaacca ctcaatccga tagcatttca gctggggccg ttagctgtcc
    20401 attggtacgg aatcattatc ggtctcggtg ctttgctcgg gctgtggatt gcgatgaggg
    20461 aaagtgaaaa acgggggctg cagaaagata cgtttattga tcttgtcctg tttgccattc
    20521 caattgcgat tatttgcgca cgaatttatt acgttgcctt tgaatgggat tattatgcgg
    20581 cacatccggg agaaatcatt aagatttgga agggcggcat tgccattcat ggcggattaa
    20641 tcggcgccat tttaacggga tatgtgtttt cgagagtgaa aaacctttcg ttctggaagc
    20701 ttgcagatat tgccgcgccc agcattttgc tcggccaggc gatcggc
//
