LOCUS       NZ_FQTY01000005        20715 bp    DNA     linear   CON 07-APR-2024
DEFINITION  Tissierella praeacuta DSM 18095, whole genome shotgun sequence.
ACCESSION   NZ_FQTY01000005
VERSION     NZ_FQTY01000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Tissierella praeacuta DSM 18095
  ORGANISM  Tissierella praeacuta DSM 18095
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae;
            Tissierella.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900128955.1-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 13:22:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,187
            CDSs (total)                      :: 3,132
            Genes (coding)                    :: 3,083
            CDSs (with protein)               :: 3,083
            Genes (RNA)                       :: 55
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 1, 4 (5S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 13 of 49
            Pseudo Genes (incomplete)         :: 34 of 49
            Pseudo Genes (internal stop)      :: 8 of 49
            Pseudo Genes (multiple problems)  :: 5 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:14:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 130295
            Orig. end                         :: 151010
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FQTY01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20715
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="1"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20715
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20715
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10715](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10715)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     gene            complement(223..1626)
                     /gene="gcvPA"
                     /locus_tag="BUB33_RS07680"
                     /old_locus_tag="SAMN02745784_01565"
     CDS             complement(223..1626)
                     /EC_number="1.4.4.2"
                     /GO_function="GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA]"
                     /GO_process="GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="gcvPA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586699.1"
                     /locus_tag="BUB33_RS07680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN02745784_01565"
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="WP_084725270.1"
                     /transl_table=11
                     /translation="MTIRGFKNHPYIPNSDLEVQREMLEKIGLKSLDELHAEVPEELKL
                     KRPLNLPKPFESEYELKRGIDKILRKNISSKEYLNFLGAGCWQHYVPAVCDEINSRSEF
                     LTAYAGEPYNDHGRFQTLFEYESLLAELLDMDVVNVPTFDWAQGAATSLRMSSRITERS
                     EALVVGTISTERLMIIKNYCDPGVKITLIDYDKETGKMDLEDLRKKISDNTAAVYFENP
                     SYLGFIEDQGAEISKIAHEAGAISVVGVDPSSLGVIAPPSSYGADIICGDLQPLGMHMN
                     YGGGQSGFISTRDEERFVMEYPSRLFGVAPTIKEGEYGFGDIAYERTSFGNLREKGKEY
                     VGTQTALLGITAGVYLSLMGPKGMVELGQNIIQKSLYAIEELSKVKGVKGSRFNNIGFK
                     EFVVDFNNTGKTVNEINKKLLDEGIFGGKDLSEEFPELGQSALYCVTEVHLKEDIDTLV
                     NAIRKIIEG"
     gene            complement(1648..2424)
                     /locus_tag="BUB33_RS07685"
                     /old_locus_tag="SAMN02745784_01566"
     CDS             complement(1648..2424)
                     /NRPS_PKS="Domain: PKS_KR (10-128). E-value: 1.5e-13.
                     Score: 43.0. Matches aSDomain:
                     nrpspksdomains_BUB33_RS07685_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584419.1"
                     /locus_tag="BUB33_RS07685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN02745784_01566"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_072975055.1"
                     /sec_met_domain="adh_short (E-value: 2.4e-32, bitscore:
                     106.0, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 2.5e-32, bitscore:
                     106.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNPLNLEGKVAVISGGASGIGLGTGKLLAQHGAKVVLLDVNQEK
                     GDSAAKEIINEGSFAIFKKCDVTNNEDCKNTIKSIEEEFGRIDILFNNAGVTVRKTVVD
                     LTEEEWDFVIDVGLKGTYLLSKYTIPIMAKNGGGSIINTGSGWGLKGGDKAAVYCAVKG
                     GIVNLTRAMAIDHGPQNIRVNSINPGDTDTAMLRDEGRQLGEKEIDAFLVESAKGRPLE
                     RLGTPLDIAKAVLFLCSDLSTWVTGTALVVDGGGIA"
     aSDomain        complement(2041..2394)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BUB33_RS07685_PKS_KR.1"
                     /evalue="1.50E-13"
                     /label="BUB33_RS07685_PKS_KR.1"
                     /locus_tag="BUB33_RS07685"
                     /protein_end="128"
                     /protein_start="10"
                     /score="43.0"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VAVISGGASGIGLGTGKLLAQHGAKVVLLDVNQEKGDSAAKEIIN
                     EGSFAIFKKCDVTNNEDCKNTIKSIEEEFGRIDILFNNAGVTVRKTVVDLTEEEWDFVI
                     DVGLKGTYLLSKYT"
     CDS_motif       complement(2311..2388)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BUB33_RS07685_0001"
                     /evalue="1.50E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="BUB33_RS07685"
                     /protein_end="38"
                     /protein_start="12"
                     /score="14.4"
                     /tool="antismash"
                     /translation="VISGGASGIGLGTGKLLAQHGAKVVL"
     misc_feature    complement(2479..2492)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.54"
                     /tool="antismash"
     gene            2647..4359
                     /locus_tag="BUB33_RS07690"
                     /old_locus_tag="SAMN02745784_01567"
     CDS             2647..4359
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008134 - transcription factor binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1112: sigma-54
                     dependent trancsriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /locus_tag="BUB33_RS07690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN02745784_01567"
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="WP_072975057.1"
                     /transl_table=11
                     /translation="MIDLKSLKSSLANIVSTMANLTNMEYAIFNTNAELVSSTQVYLQR
                     KGLKVHLTSIEEVLNQGNVIVNKPGLMKSCVGCRFVNNCPSTIEILSCIKLNGEPIGVV
                     SLTSFSQEGHNMIEENIRIYTDMLEDISNLISMFAFNENSKKDTQVLHEIISNIIEETE
                     DNYLIVNNRGLLIHWTKGIEELFSYCDLYTQTIDLMFPKDITNWIFNEEKQGKKYCVIE
                     GFKGILYSRPLKIEEDIVGYILKLEKDKNSSNSHIKEDYLGSIISNDYKIENIKSQIKK
                     ISNSSSSVLITGDTGTGKEVVAKAIHYTSKRANNPFIPINCANIPDSLFESELFGYEEG
                     AFTGAKKGGKLGLFELANGGSIFLDEIGELPIYLQAKLLRVLQENAIQRLGSITNIPVD
                     IRIIAATNQDLESMVNENKFRADLFYRLNVIPIYLPPLNQRLSDIDILSTHFIEKYNRK
                     LMKNINNISKEALELMKSYSWPGNVRELENTIEYAINMEDTEIIHIESLPHRIRKKANG
                     KNDFKELIAQKEADLIINVLNKHGWDLEGKQKASKELGISIRTLYRKLKELEI"
     misc_feature    complement(4453..4471)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 24.62"
                     /tool="antismash"
     misc_feature    complement(4456..4471)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 25.38"
                     /tool="antismash"
     gene            4508..4732
                     /locus_tag="BUB33_RS07695"
                     /old_locus_tag="SAMN02745784_01568"
     CDS             4508..4732
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588420.1"
                     /locus_tag="BUB33_RS07695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN02745784_01568"
                     /product="ferrous iron transport protein A"
                     /protein_id="WP_072975059.1"
                     /transl_table=11
                     /translation="MSTLSLVSLKPNECGRIKEISGCCKAKKRLYELGLHKNAYVKVIK
                     NDFGPLIISLSGNKLALGRGLACHIIVER"
     misc_feature    complement(4749..4764)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 23.94"
                     /tool="antismash"
     gene            4783..6777
                     /gene="feoB"
                     /locus_tag="BUB33_RS07700"
                     /old_locus_tag="SAMN02745784_01569"
     CDS             4783..6777
                     /GO_function="GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="feoB"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /locus_tag="BUB33_RS07700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN02745784_01569"
                     /product="ferrous iron transport protein B"
                     /protein_id="WP_072975062.1"
                     /transl_table=11
                     /translation="MNTIALVGNPNSGKTTLFNALTGSNQHVGNWPGVTVEKKEGKFKY
                     KGNHYNVVDLPGTYSLGAYSEDEVVARDYILKGNPDIVINVVDATNIERNLYLTTQLIE
                     MGAKVIIALNMMDEAKQKNIEIDLEKLSKELGVPIIPTVASKSKGLDMLIEKSIQYAEN
                     KENPNLKFSYGEDIDKEIEDINSMLSNSNLEYPGKWVAIKLLEGDKEVYRLIENKGTAP
                     IKERLISLEEKSTEYELQIVDKRYDFINTIVNKTVKKPSDTVETPTDKIDKIITHKYLG
                     LPIFALIMFTIFQLTFVLGQDILGGYVATIVETIGEYVVNLLVMINAPEWVQSFISEGI
                     FAGVGAVFEFVPLITVLYFFIGILEDTGYMARAAYVMDGLMRTLGLHGKTFISMIVGFG
                     CNVPGIMSARTLDNKKDKMIAILINPFMSCGARLPIYLVFIAAFFPKHGGIVLFSLYTL
                     GIIMALLVGKIFSKTLFKGESSHFIMELPPYRLPIFKYVIRDMWDKVWDFLYRAGTIIF
                     VVVSLLWVLSVLPFGVEPYSQESILGKIGTILAPIFIPAGFGTWQATVSLFAGIAAKEA
                     VVAVLGMVYAGVSEGSQLVSALQNVFTPLTAISFMVMVLLYTPCAAVIGTVKKETNSTK
                     WAVFMTVYPFIIGWISSVLVYQVGRLLGF"
     gene            6789..7049
                     /locus_tag="BUB33_RS07705"
                     /old_locus_tag="SAMN02745784_01570"
     CDS             6789..7049
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /locus_tag="BUB33_RS07705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN02745784_01570"
                     /product="HTH domain-containing protein"
                     /protein_id="WP_072975065.1"
                     /transl_table=11
                     /translation="MLRDVLKEISNAKVFSVSLIAKKLNISETLVEDAVKELSRMNYII
                     EDMGSPTCETKCSGCSMKAFCNTVPIKTISLTDKGMKLLKH"
     misc_feature    7081..7096
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.71"
                     /tool="antismash"
     gene            7475..8929
                     /locus_tag="BUB33_RS07710"
                     /old_locus_tag="SAMN02745784_01571"
     CDS             7475..8929
                     /EC_number="6.3.4.21"
                     /GO_function="GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588422.1"
                     /locus_tag="BUB33_RS07710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN02745784_01571"
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="WP_072975067.1"
                     /transl_table=11
                     /translation="MISLDWKERKNLTMLADFYEFTMANGYLENNMENQIGYFDMFFRS
                     IPDDGGFAIMAGVEQLIEYLRNLKFNEEDIAYFESKKIFGEKFLNYLRNFKFKCDVWAI
                     PEGTPIFPQEPIVIVRGPVVQAQLIETMVLLTINHQSLIATKANRIVRAANGRTVMEFG
                     SRRAQGYEGAILGARAAYIGGCIGTANTIVDRDFNIPALGTMAHSWVQMFPTELDAFRA
                     YAKIYPDNCTLLVDTYNTLKEGIPNAITVFNEEIVPLGFRPKGIRIDSGDIAYLSKEAR
                     KMLDEAGFPDCMIVASSSLDEYVIRDLLIQGAQIDSFGVGERLITAKSQPVFGGVYKLT
                     TIEKDGKLIPTIKVSENVGKITTPGFKQVYRLFDKATNKAIADVITLHDEIIDDTKPYE
                     IFHPLYTWKRKTLENFYSKKLLVKIFEDGHCIYENPTIHEIKSYCKDQLDLMWDEVKRF
                     ERPHRYFVDLSQKLWNIKDELLKKHK"
     gene            complement(8980..9555)
                     /locus_tag="BUB33_RS07715"
                     /old_locus_tag="SAMN02745784_01572"
     CDS             complement(8980..9555)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BUB33_RS07715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN02745784_01572"
                     /product="hypothetical protein"
                     /protein_id="WP_072975070.1"
                     /transl_table=11
                     /translation="MDRINENLRYIFVLFIILFIWMSIIYKVSSKYLFSTPPIKYENLA
                     LDIELKEGRPIEIKEFIYDDHDKKYLYVIYSIKNSNEIGLVIYEKVFINKYIRLAANKA
                     SPYLFNIYYITDACKDKHQLYIVYGKNNNRRISHFELDFGDIVKKEDISQKDYYLIVNR
                     FKDKNMEFPKTTFFDSKNIDITRYILEK"
     misc_feature    9844..9853
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     gene            complement(10001..10132)
                     /locus_tag="BUB33_RS07720"
                     /old_locus_tag="SAMN02745784_01573"
     CDS             complement(10001..10132)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: TIGR04223"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR04223.1"
                     /locus_tag="BUB33_RS07720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN02745784_01573"
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="WP_159429176.1"
                     /sec_met_domain="TIGR04223 (E-value: 1.3e-10, bitscore:
                     34.7, seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKVLSKGMEKISKVAKKVAEESPNTISAFLYYEPKAPKALKK"
     gene            complement(10113..10715)
                     /locus_tag="BUB33_RS07725"
                     /old_locus_tag="SAMN02745784_01574"
     CDS             complement(10113..10715)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /locus_tag="BUB33_RS07725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN02745784_01574"
                     /product="accessory gene regulator B family protein"
                     /protein_id="WP_115322109.1"
                     /sec_met_domain="AgrB (E-value: 9.1e-30, bitscore: 96.9,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINQFIRYCFSQSTMDEDEAEIIFYGMKIIGTSILTSLIVILIGI
                     IMGQAVSAVIYLGTLILLRRNVGGYHSKTYLGCLSVTSINFIIIVFLEKWLNQNLKEII
                     GIIFIIYATIKIYLSKPTVHKNRIVNQEIINKSNTKKDKWLSVILALATICHILTVLGL
                     VNEINYFFAISSSLMIVALSINNKTKGEEDGYEESFK"
     misc_feature    complement(10738..10751)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 25.44"
                     /tool="antismash"
     gene            complement(11087..11473)
                     /locus_tag="BUB33_RS07730"
                     /old_locus_tag="SAMN02745784_01575"
     CDS             complement(11087..11473)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BUB33_RS07730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN02745784_01575"
                     /product="hypothetical protein"
                     /protein_id="WP_072975076.1"
                     /transl_table=11
                     /translation="MKRKLGTCLSMMILLSVLFSYNTIFAENNFNRQSEGQYTQDSIKH
                     SPNLSQEEYEEYIKANPEPLFGGKFISIQKRYSSKNNIPAAVEYSEYTDGYWWSGVLAR
                     VGSITIDPITGYYLATFEGKIYKQ"
     misc_feature    complement(11831..11844)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 25.44"
                     /tool="antismash"
     gene            complement(12034..12198)
                     /locus_tag="BUB33_RS16035"
                     /old_locus_tag="SAMN02745784_01576"
     CDS             complement(12034..12198)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BUB33_RS16035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN02745784_01576"
                     /product="hypothetical protein"
                     /protein_id="WP_159429177.1"
                     /transl_table=11
                     /translation="MEILRDERSLRDLIVAGCGAGCVQGCSCGRGCSSGSGCGCGCGCN
                     CGGVPCLVE"
     misc_feature    complement(12233..12248)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 20.67"
                     /tool="antismash"
     gene            complement(12335..14158)
                     /locus_tag="BUB33_RS07735"
                     /old_locus_tag="SAMN02745784_01577"
     CDS             complement(12335..14158)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="BUB33_RS07735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN02745784_01577"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_072975078.1"
                     /transl_table=11
                     /translation="MDSKKNYKNNIKNNFYFFKYVFEFSKSYVISQGIISIIEGLIPLI
                     YIIIPKLIIDGLINRKNFSEIVNYILIYILIQLTASLITAILTEKYVNLNGHLYAMHFL
                     LIINKKVVSLDMAQLDLPETHQKLALAQDIIYKGIGINLINSLFHSLSSIVLIISTTII
                     IISADINLIFVIILFSFFSVYLNLKSENWKISQRDENIYLTRILNYYIRIMGESSSAKE
                     MKMFGFVDWIMNKYLTTLSFLRIRLKKLYNTSMNIKIVSIIGENLKSNGIYLYLAWKTF
                     IGNITVGQFSQYFTATSQLSSAILNFVGFITQLNINGKYIESFREFMELESDIKRNNDI
                     LLKNGEIKTIESVLKGNDLTIRLENVYFAYPGTDKYVLENINYVFEYGKVYVIVGENGA
                     GKSTLINLISGLYEPTSGTIYLNNIPINQIDYMDYRNIFSIVFQDFKYFSFTIAENVAL
                     NKSNDSNEFKISSYLQEVGLGGKIKTLPKGIHTNLDKIFYEDGVILSGGENQKLALARA
                     LFRETPVIMLDEPSSALDPISEDDLLSKFKNLAADKLVIYISHRMTCASTADQIIFIKN
                     KTIFEQGSHRELIDKNGDYAKYYQTQAKYYV"
     gene            complement(14247..14474)
                     /locus_tag="BUB33_RS15820"
                     /old_locus_tag="SAMN02745784_01578"
     CDS             complement(14247..14474)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BUB33_RS15820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN02745784_01578"
                     /product="hypothetical protein"
                     /protein_id="WP_115322108.1"
                     /transl_table=11
                     /translation="MEIVRNSCFNGNDNPGMGCGCGCQNGYGCGCTGFPPGNGGDYPGY
                     GCGCNCSGEGGGNTCPPNGMGCGCGCGTAN"
     gene            complement(14844..16181)
                     /locus_tag="BUB33_RS07745"
                     /old_locus_tag="SAMN02745784_01579"
     CDS             complement(14844..16181)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04085"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /locus_tag="BUB33_RS07745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN02745784_01579"
                     /product="radical SAM protein"
                     /protein_id="WP_072975084.1"
                     /sec_met_domain="PTHR43273 (E-value: 6.3e-44, bitscore:
                     143.9, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 4.5e-10, bitscore: 33.5,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.4e-15, bitscore: 51.6,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 3.9e-12, bitscore:
                     40.0, seeds: 281, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKLMIKPNVLKKSYYNFFIPKEDGTYLVYNSLSGAIIYIYSEIN
                     VRKLREIMEKDTIIYDNSDEMILTFYEKGILVPKDRREYEFARYCYERDVVRDTTLVLT
                     LIVTRQCNLRCIYCYEEHENKAMTEDLYSSILKYIDNSLRNKLYTGVAISLFGGEPFVE
                     YDKVVAFLGKVKSLCEEYGIPYSASATTNGALIYPERFEVLSSLNCRYYQITVDGFKET
                     HDKYRASVDGRGSWDKIMENLKYMASTDYNFKVTIRTNFNDEVFLRAEEFYKYIKENFD
                     NRFSIYYEGIKRLGGCNDDKLDILDKLDVAKSSVNIAKVIKQLNINNDVVDVMTRPYSR
                     VCYASKHNNLIIDYDGSILKCTLSLDDDLNKIGYISEDGSMNLAEDKHSRWISNKVDLQ
                     DECKECRVLPICFGGRCVNGRVHGEKYFCDPKIEESELENLISTYR"
     gene            16517..17242
                     /locus_tag="BUB33_RS07750"
                     /old_locus_tag="SAMN02745784_01580"
     CDS             16517..17242
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /locus_tag="BUB33_RS07750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN02745784_01580"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_072975086.1"
                     /transl_table=11
                     /translation="MFKIGICEDNKIFSLELKSIIERKFTQYNLESKINCFYSGEDTLE
                     GILNAKERYDIIFFDIELPKLNGIEVARKIRELYDDTIFIFITYLDEKVYEALDLTIFH
                     FIRKSHFQKEINLILDSLIKKLDYLTEKYAFPIGEDNVYFRLYDILYLEILNRQLIIHT
                     KENVYTSTYRSLKDISFDLTKKHFYEVYRGIMVNLNHVKDFIDNKIILSNGDIVYIARR
                     RLNGFKEEFYKYISSKREG"
     gene            17245..18573
                     /locus_tag="BUB33_RS07755"
                     /old_locus_tag="SAMN02745784_01581"
     CDS             17245..18573
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /locus_tag="BUB33_RS07755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN02745784_01581"
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_072975088.1"
                     /transl_table=11
                     /translation="MSRLNIILNFLFCIIDAMIFINLLYIFKDNKSEYKFTDNISIIAG
                     ISIAIFFFTQLGIIPYTKVILISIVLFFITFLYELRLYQQLLIVILYYFILIISELLIT
                     LLASNILSVGVDSIGKHNYSFFFLGTISKFLSILILSLVKRRFANRKIILPIILNYIII
                     LILLISTISMVLLFYSNLNSHSKSIQFILFLVCLFTLFTSIGILIIYFYANDFYIDLQK
                     ETTKKIYDKSYEKFIANAELRNDSLAKIWHDIGNHIKVLEKMNKIENNTDIEYINSIKN
                     RLKSIPNTINTGNKLIDIILNDKYSEGIMKGIDFDIKAIAPPKIDIDDMDLSSILFNTI
                     DNAIEACLNYSGKNKYIYLELYPDGNFLCYKIKNTYTPIKNTSVKNFYFNKKQYISSGY
                     GLSIIKDIVNKYDGYMDINKDDKEYSVTIVLHLNNQSVQEITT"
     misc_feature    complement(18605..18618)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     strong, score: 28.14"
                     /tool="antismash"
     misc_feature    18606..18619
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 25.44"
                     /tool="antismash"
     gene            18648..18833
                     /locus_tag="BUB33_RS07760"
                     /old_locus_tag="SAMN02745784_01582"
     CDS             18648..18833
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BUB33_RS07760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN02745784_01582"
                     /product="hypothetical protein"
                     /protein_id="WP_072975090.1"
                     /transl_table=11
                     /translation="MKDFVKDFFTKRLELNFINILMIALLVYVYNLSGNKYFLVYLFFF
                     RLIALCIDYLTDYLKN"
     gene            complement(18991..19182)
                     /locus_tag="BUB33_RS07765"
     CDS             complement(18991..19182)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BUB33_RS07765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_072975093.1"
                     /transl_table=11
                     /translation="MIGIARKLYDTNLKRFYYNGGPYRNYEGDRGRESIWAYEDRDYKY
                     YIDNMGDEFDFYWDDYDL"
     gene            19356..19535
                     /locus_tag="BUB33_RS07770"
                     /old_locus_tag="SAMN02745784_01583"
     CDS             19356..19535
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BUB33_RS07770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN02745784_01583"
                     /product="hypothetical protein"
                     /protein_id="WP_072975096.1"
                     /transl_table=11
                     /translation="MGYLIILNNKYSERTMKNIDFDIKTIILLEIDIDNMDLSSILFNT
                     IDNAIEAYLNYSGK"
     misc_feature    20066..20077
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
ORIGIN
        1 tggtaaatta ggtgctccat ttcttctcat agataaagga agtttagaca atccatctcc
       61 cacttgatta attatagctt cttctgcctt gggaactaac actccccttt cgcccttgcg
      121 acttaattca aatataattg gctcattcca tcttgcttga tgaaagtctc taaccttaga
      181 gcttctcttt attctcttca tttaatcaat cctttctata gattatcctt cgatgatttt
      241 tctaatggca tttactaaag tatcaatatc ttcctttaaa tgaacttcag taacacagta
      301 caaagccgat tgacctaatt cagggaactc ctcagataaa tctttgccac caaatatacc
      361 ttcatctagt aatttcttat ttatctcatt tacggttttt ccagtattat tgaaatcaac
      421 tacaaattct ttaaatccta tattattaaa ccttgagcct ttaacgcctt ttactttaga
      481 tagttcttct atagcgtata aggatttctg tataatattt tgccctaatt caaccattcc
      541 cttaggtccc attaaagaca aataaactcc tgctgtaata ccaagcagag cagtttgtgt
      601 acctacgtat tccttacctt tctctctcaa gtttccaaag gaagttcttt cataggctat
      661 atctccaaat ccatattctc cttcttttat agtgggagct acaccgaata atctagatgg
      721 atactccatt acaaatcttt cttcatctct agtagaaata aaacctgatt gtcctccacc
      781 ataattcata tgcatcccca aaggttgaag atctccacat ataatatctg ctccatagga
      841 tgatggaggt gctataactc ccaagctact tggatcaaca cctactactg atattgcacc
      901 agcttcatga gctatcttag aaatctcagc cccttggtct tcaataaatc ctaaatatga
      961 aggattctca aaatatactg ctgcagtatt atcagaaatc tttttcctca aatcttctaa
     1021 atccatcttt ccagtttctt tatcataatc aattaaagta attttaaccc ctggatcaca
     1081 ataatttttt attatcatca acctttcagt acttatagtc ccaacaacta aagcttcaga
     1141 cctttcagta attctagatg acatcctgag ggatgtagca gctccttgag cccaatcaaa
     1201 ggtaggaaca tttacaacat ccatatcaag taattccgct aataaggact catattcaaa
     1261 caaagtctga aatctaccat ggtcattata tggctctcca gcataagcag tcaaaaattc
     1321 agatctgcta tttatttcat cacaaactgc tggtacataa tgctgccagc atcctgctcc
     1381 tagaaaattc aaatattcct tagatgatat attctttctc aaaattttat ctatgcctct
     1441 ttttaattca tactctgact caaaaggctt tggcaaattc aacggtcttt ttaattttaa
     1501 ttcttctggt acttctgcat gtaattcatc taaagatttt aatcctattt tttctaacat
     1561 ttccctttga acttctaaat ctgaatttgg aatataggga tgatttttaa atccacgtat
     1621 tgtcatactt ctacctccta aattttatta tgcaattcct ccaccatcta caactaaagc
     1681 agttccagta acccaagttg ataaatcact acataaaaac agtactgcct ttgcaatatc
     1741 caatggtgtt cccagtcttt ctaggggtct gcccttagct gattccacaa ggaaagcatc
     1801 tatctccttt tctccaagct gtcttccttc atctcttaac attgctgtgt cagtatcccc
     1861 aggatttata gagttaactc taatattttg tggtccatga tcaattgcca tggctcgagt
     1921 taaatttaca attccaccct ttacagcaca gtaaacagcg gccttatctc caccttttag
     1981 tccccagcct gaaccagtat ttattatgct tcctcctcca tttttagcca taattggtat
     2041 tgtatattta gataataaat atgttccttt aagtcctaca tcaattacaa aatcccattc
     2101 ttcttctgtt aaatctacta ctgtctttct aacagtaacc cccgcattat taaataatat
     2161 atcaattctg ccaaattctt cttctattga tttaatagta tttttacaat cttcattatt
     2221 agtcacatcg catttcttga aaatagcaaa actaccctca ttaatgattt ctttagctgc
     2281 cgagtctcct ttttcttgat taacatcaag tagcacaacc tttgcaccat gttgagctaa
     2341 taattttccc gttcccagtc caataccaga agctccacct gaaataaccg ctacttttcc
     2401 ctctaaattt aatggattat tcataattct ccccctcaca aaatagtttt cgccaaatgc
     2461 tattggtatt actatcttgc ataaatcatg ccaaaaaatt agataaaaaa tatcttttag
     2521 caatctacct attttttcag acttttagca attttataat tgttttattg agttaatgtg
     2581 gtataattaa attgtcttta ttgacaaata tagcaacttt tttgctagat atggcaggtg
     2641 cctgatatga ttgatttaaa aagcttaaaa agtagtttag caaatatagt atcaacaatg
     2701 gctaacttaa ccaatatgga atatgcaata tttaacacca atgctgaatt agtaagtagt
     2761 acacaagttt atttgcaaag gaaaggtcta aaagtacatt taacctctat agaagaagtc
     2821 ttaaatcaag gaaatgtaat tgtgaataaa cctggactta tgaaatcatg tgttggatgc
     2881 agatttgtta ataactgtcc ttctactata gaaattctat cctgtataaa actaaatgga
     2941 gaacctatag gtgtagtgag tctcacatca ttttctcaag aaggacataa tatgattgaa
     3001 gaaaacattc gaatatatac agatatgttg gaggatattt caaacttaat atctatgttt
     3061 gcatttaatg aaaactcaaa aaaagatact caagtcttac atgaaataat tagtaacatt
     3121 attgaagaaa cagaagataa ctatcttata gtaaataata ggggtctttt aattcattgg
     3181 actaaaggta ttgaagaact attttcttat tgcgatttat atacacaaac tattgattta
     3241 atgtttccta aagacattac taattggata tttaatgaag aaaaacaggg gaaaaaatat
     3301 tgtgttatag aaggatttaa aggaatttta tactccagac ctttaaaaat agaagaagat
     3361 atagtgggtt acattctaaa attagaaaaa gataaaaata gttcaaattc acatattaaa
     3421 gaagattatt taggctccat tatctccaat gattacaaaa tagaaaatat aaaatcacaa
     3481 ataaaaaaaa tatccaattc atcttcttca gttttaataa ctggtgatac aggcacagga
     3541 aaggaagtag tggcaaaagc aattcactat acaagtaaaa gagctaataa tcctttcatt
     3601 ccaattaact gtgctaatat accagactct ttatttgaat ctgaactctt tggatatgag
     3661 gaaggtgctt ttactggtgc aaaaaaaggt ggtaaattag gtttgtttga attggctaat
     3721 ggtggttcaa tctttttaga tgaaattggt gaattgccaa tttacttaca agccaaatta
     3781 cttagagttc ttcaagaaaa tgctatacaa agattaggta gtatcacaaa tatcccagtg
     3841 gatattagaa taatagcagc tactaatcaa gatttagagt caatggtaaa tgaaaataaa
     3901 tttagagctg atttgttcta tagattaaat gttataccta tttatttacc tccattaaat
     3961 caaagattat cggatataga catactctca acccatttta tagaaaaata taatagaaaa
     4021 ctaatgaaaa acattaataa tatttccaaa gaagccttgg aacttatgaa atcatattca
     4081 tggccaggaa atgtaagaga acttgaaaac actatagaat atgcaattaa tatggaagat
     4141 acagaaatta ttcatattga aagccttccc catagaataa gaaaaaaagc taatggaaaa
     4201 aacgatttta aagagctaat tgcacaaaaa gaagcagatt taattataaa tgtcctcaat
     4261 aaacacggtt gggatttaga aggaaaacaa aaagcatcaa aagaattagg aattagtatt
     4321 agaacattat atagaaaact caaagaactg gaaatatagg ttcctgaaat aatagacaag
     4381 tagcttatat gtaagttgcc tgtctataat aaatttattg catttatctg agaaatatat
     4441 tatacttata acgttgataa tcgttatcat tatggcttat tttgtataaa atggaaggat
     4501 gatatttatg agcactttat ctttagtttc tttaaagcct aatgaatgtg gaagaattaa
     4561 ggaaatctca ggatgctgta aggctaaaaa aaggttgtac gagcttggtc tccataaaaa
     4621 tgcctatgtt aaagttataa aaaatgattt tggtcctcta atcattagtt tatccggtaa
     4681 taaattggct ctaggtcgcg ggttagcctg tcatattata gttgagcgtt aattttttgt
     4741 tattagttga taatggctat catttataaa gggggaattt ttttgaatac cattgctctt
     4801 gtaggtaatc ctaatagtgg aaaaactaca ttgtttaatg ctctaactgg ttccaatcaa
     4861 catgttggca attggcccgg agttactgtt gaaaaaaaag aaggaaaatt taaatataaa
     4921 ggaaatcact ataatgtcgt cgacttgccg ggaacttata gccttggagc ttattcagaa
     4981 gatgaagtag ttgctcgtga ctatatactt aaaggaaatc cagatattgt aattaatgta
     5041 gttgatgcta ctaatataga aagaaattta tatttaacaa cacaattaat cgaaatgggt
     5101 gcaaaggtta ttatagcact taatatgatg gatgaagcca agcaaaaaaa tattgaaatt
     5161 gatttagaaa aattatctaa agagctagga gttccaataa tccctactgt agcttccaaa
     5221 tctaaggggt tagatatgct aatagaaaaa tctattcaat atgcggaaaa taaagaaaat
     5281 cctaatttga aatttagtta tggagaggac attgataaag aaattgaaga tataaactca
     5341 atgcttagta attcaaactt agaataccct ggcaagtggg tggcaataaa actcttagag
     5401 ggtgataagg aagtctatag acttatagag aataaaggta ctgcacctat taaagaaaga
     5461 ttaatatcat tagaggaaaa gtccacagaa tatgaattac aaattgtaga taaaagatat
     5521 gattttataa acactattgt aaataagact gtgaaaaaac catctgatac agtagaaact
     5581 ccaactgata aaatagataa gattattaca cataaatatt taggacttcc tatatttgct
     5641 ttaattatgt ttaccatatt tcaacttaca tttgttcttg gtcaagatat acttggtgga
     5701 tatgtagcaa ctattgtgga aaccattgga gaatatgtag ttaatctatt agttatgata
     5761 aatgctcctg aatgggtgca atcttttatt tctgaaggta tatttgctgg ggttggtgct
     5821 gtatttgaat ttgttcctct cattacagta ttatattttt ttataggtat attagaggat
     5881 acaggttata tggctagagc tgcttatgta atggatggat tgatgagaac ccttggtctt
     5941 catggtaaaa cttttatttc catgatagta ggctttggat gtaatgtccc tggaataatg
     6001 tctgctagaa ccttagataa taaaaaggat aaaatgattg ctattcttat taatccattt
     6061 atgtcttgtg gagcaagatt acctatttat ttagtgttta ttgctgcgtt tttccctaaa
     6121 catgggggaa tagtattgtt ttctctttat actctcggta ttataatggc tttattagtt
     6181 ggtaaaatat tttcaaaaac actatttaaa ggtgaatctt cacattttat tatggaacta
     6241 cctccttata gactacctat atttaagtat gttataaggg atatgtggga taaggtatgg
     6301 gatttcttat atagagctgg aactattata tttgttgtag tttctctact ttgggtttta
     6361 tctgtattgc catttggagt agaaccctat agtcaagaaa gtattttagg taaaataggt
     6421 actattttag ctcctatatt tatacctgct ggatttggta cttggcaagc tacagttagt
     6481 ttgtttgcag gtatagcagc taaggaagct gtagtagctg ttctaggtat ggtttatgct
     6541 ggagtatctg aaggttctca gctagttagt gctttacaaa atgtatttac tcctcttact
     6601 gctatatctt ttatggtaat ggtactttta tacacaccct gtgccgcagt aataggtact
     6661 gtaaaaaagg aaactaattc tactaaatgg gcagtattta tgacagttta tccttttata
     6721 ataggttgga tatcctctgt acttgtttac caagttggta gattattagg tttttaggag
     6781 ggaatttaat gcttagggac gtcttaaaag aaatatctaa tgctaaagta ttttctgttt
     6841 ctttgatagc aaaaaagtta aatatatctg aaactttagt tgaagatgca gtaaaagaat
     6901 tatctagaat gaattacatt atagaagata tgggttcccc tacttgtgaa acaaaatgca
     6961 gcggatgctc aatgaaagct ttttgcaata cagtcccaat aaaaaccatt agtttaacag
     7021 ataagggaat gaagttgtta aaacattaat ataacttttt gtaatttttc aatattttac
     7081 ttgactaact tgttaaagcg atgtataatt aatttaaata aaactttaac ttttccaccc
     7141 taaagattta aaaaatttga tagtttattt tattagtaag gattggaaaa acaatagtta
     7201 gtttgtagta taataaaaaa gtattctgtg tagtaaaatg tagaaacctt ttaaaccctc
     7261 attaaatcaa cgtaaggagt gtatcagaag attgaattta gtagttgaat taaatcaatc
     7321 aggttggaaa ggttgtaaaa agttttattt taaataatag gtagtttcta gagatattag
     7381 agactaccta tttatttttt gttatttcct ttgttgaact ttctttgcta taggtctata
     7441 attagagtaa caatatataa aaaggagact accgatgata agcttagatt ggaaagagag
     7501 aaaaaatctt actatgttag cagatttcta tgaattcact atggccaacg gatatctaga
     7561 aaataacatg gaaaatcaaa ttggatattt tgacatgttt tttaggagta taccagatga
     7621 tggtggattt gctattatgg ctggtgttga gcaattaata gaatatctaa gaaatcttaa
     7681 gttcaatgag gaggacattg cctattttga atccaagaaa atttttggag aaaagttttt
     7741 aaactattta agaaatttta agtttaaatg cgatgtatgg gcaattccag aaggtactcc
     7801 catatttcct caagaaccta tagtaattgt aagaggacct gtagttcaag ctcaacttat
     7861 agaaaccatg gttcttctaa ctataaacca tcaaagttta attgctacta aagctaatag
     7921 aatagttagg gctgcaaatg gaagaactgt aatggaattt ggttctagac gtgctcaggg
     7981 ttatgaaggt gctatccttg gtgctagagc tgcttatatt ggcggatgta tcggaaccgc
     8041 taataccata gtggataggg attttaatat tccagcattg ggaactatgg ctcatagctg
     8101 ggtacaaatg tttcctactg aattagatgc ttttagggca tacgctaaga tttatccaga
     8161 taactgtact cttctagtcg atacttacaa taccttaaaa gaaggcattc caaatgcaat
     8221 aacagtattt aatgaggaaa ttgtaccatt aggatttaga ccaaagggaa ttagaataga
     8281 tagcggagat attgcttatc tatctaagga agcccgcaaa atgcttgatg aagctggatt
     8341 ccctgattgc atgattgtag catcatccag tttagatgaa tatgttataa gagatttatt
     8401 aatccaaggt gcacaaattg attcttttgg cgttggtgaa agacttataa ctgcaaaatc
     8461 tcaacctgtt tttggcgggg tctataaatt aactacaata gaaaaggacg gaaaacttat
     8521 accaacaatt aaagttagtg aaaatgtagg taaaataact acaccaggat ttaaacaagt
     8581 ttatagatta ttcgacaaag caacaaataa agctattgct gatgtcatta ctttacatga
     8641 tgaaatcatt gatgacacta agccctatga aatcttccac cctttatata catggaagag
     8701 aaagacctta gaaaattttt attctaagaa actattagta aaaatctttg aagatggaca
     8761 ttgtatttat gaaaacccta ctattcatga aataaaatct tactgcaaag accaattaga
     8821 tttaatgtgg gatgaagtca agagatttga aagaccccat agatatttcg tagacttatc
     8881 tcagaaacta tggaatatta aggatgaatt attaaagaaa cataaataag tgtaaataat
     8941 aagtgctatt tttgatatca aatacaaaaa tagcacttat tatttttcta aaatatatct
     9001 ggttatatca atatttttac tatcaaaaaa agtagttttg gggaattcca tattcttatc
     9061 tttaaaccta tttacaatca aataataatc tttttgggat atatcttctt ttttaacaat
     9121 atctccaaaa tctaattcaa aatgtgaaat tctcctatta ttatttttcc cataaacaat
     9181 atataattga tgcttatcct tacaagcatc agtgatataa taaatattaa ataaatatgg
     9241 agaagcttta tttgctgcaa gtctgatata cttatttata aatacctttt catatataac
     9301 taacccaatt tcatttgaat tctttataga ataaattaca tataaatatt ttttatcatg
     9361 atcatcataa ataaattcct ttatttctat aggtctcccc tcttttaatt caatatccaa
     9421 agctaagttt tcatatttta ttggaggagt tgaaaataaa tatttactac taactttata
     9481 tataatagac atccatatga ataaaataat aaatagcaca aaaatatatc tcaaattttc
     9541 attgatacga tccaaaacat acacctcttt ttttgctaaa tcaatagaat aggaagaaac
     9601 aatattttat ctataagttc attctacatt attagaagtt atttaatatg agttcaaaag
     9661 atattaattt tagatccgtt tttatattaa ttaaatatag tgctttaaaa cctcaatgct
     9721 attaataaaa aatatatata atataggaca tataacttta aaaaatcacc actttaacaa
     9781 ttgcatctta ataataaaat gaaaattaaa aacactggtt ttcagattag ttctgaaaac
     9841 cagtgttttt tgatttaatg gatttgaaca gattaaataa tctggctact aggttttcat
     9901 tgaaaaagtc aaattattaa cctttattat attgttaatg caaatctgta gaaattaatt
     9961 atgtttgata ttaaaaagta atctttccaa aactcttgtc ttatttcttt agagccttag
    10021 gtgccttagg ttcataatat aaaaatgcag aaattgtgtt tggagattcc tctgcaactt
    10081 tttttgctac cttgcttatt ttctccatcc ctttacttaa aactttcttc atatccatct
    10141 tcctcccctt tagttttatt attaatactt aatgccacta tcattaaaga actactaatg
    10201 gcaaagaagt aattaatttc atttactaag cctagtactg ttaatatatg gcatatggtt
    10261 gctaaagcca agataacact taaccattta tcctttttcg tattgctttt atttataatc
    10321 tcttgattta caattctatt tttatgtact gtaggtttag agagataaat ttttatagta
    10381 gcataaataa taaatattat tcctataatt tcttttaaat tctgattcag ccatttttct
    10441 aaaaacacta taataataaa atttatactt gtaacagata aacatcctaa atatgtctta
    10501 ctatgatagc ctccaacatt tctcctaagt aaaatcagtg tacctaggta aatcacagca
    10561 gatactgctt gtcccataat aattccaatc aatataacaa ttagagatgt taagatacta
    10621 gttcctatta ttttcattcc ataaaatatt atttcagctt catcttcatc cattgtggat
    10681 tgtgaaaaac aatatctaat aaactgattt atcactttca atcctaaccc ctttctctta
    10741 caataattat aacaagtaaa tttttaaata gttaaaaatt ggagtaagtg gtcttttttt
    10801 ggattgaacg gttatttaag agtgaatacc ataaataaca caataaaatc ttatgtgaat
    10861 tgcagtggaa agaaaagata tatttattta aaattacatc ctcataaaaa ttccctacgt
    10921 aataaaacaa aaacatatat atgcctgtta ataataattt ccttaataaa atcaaaagca
    10981 atgtatttcc tctagacatt gcttcagtat aatagaatat attataaata aatatattgg
    11041 atttatgaat aactcgattg ttattgttct ttttaactcc tcctatttat tgtttataaa
    11101 tttttccttc aaatgttgct aaatagtacc ccgttatggg gtctatagtt atgcttccta
    11161 ctcgtgcaag tactcctgac caccagtacc catctgtgta ttcactgtat tctacagctg
    11221 caggaatatt attctttgaa gaatatcttt tttgtatact tataaatttt cctccaaaaa
    11281 gcggttcagg attagctttt atatattcct catactcctc ctgtgaaaga ttaggtgaat
    11341 gttttattga atcctgtgta tattgtccct cactttgtct attaaagttg ttttcagcaa
    11401 atattgtatt gtaagaaaaa agtacagaca aaagaatcat cattgataga caagtaccta
    11461 attttctttt cataccattt acctccttct atttttgttg ttctttttat accatatatg
    11521 tctgcttttt attactgatt tttcattctt taccaatgat ttatggtggt atttacaagg
    11581 aaaaatgtaa attctatatt tgttgaccct tctgtattct tctttacttc attacttatt
    11641 ttctccatcc ctcccactac tgttgataca tgacatattg ttgctaaagc taggataata
    11701 cttagccata tatccttttt tgtattactt ttatttataa tttcttttaa attctgattc
    11761 agccattgtg gattgtgaaa aacaatatct aataaactga tttatcactt tcaatcctaa
    11821 cccctttctc ttacaataat tataacaagt agatttttaa atagttaaaa attggagtaa
    11881 gtggtctttt tttggattga agggttattt aagagtgata ttctcaatat agttatgaat
    11941 aaaataaggt acatacataa ctccatatga gtactcatat atacatctgc ttatattaga
    12001 aaaaaagagg gctgtttaac cctcgataat cagttattca actaaacatg gtactccacc
    12061 acaattacag ccacaaccac atccacagcc actcccagaa ctacaaccac gtccgcagga
    12121 acatccttgt acacagcctg caccacatcc tgcaactatc aaatctctta aacttctctc
    12181 atctcttaaa atttccataa tcttattcct cccctcatat aatatatatc actattccca
    12241 tggggtaata atacaggtag atgttgtgat ttcctttttc caccaaacca accaagtaat
    12301 tactcatatc ataggaatta gagatgtctt cattttaaac atagtatttc gcctgggttt
    12361 gataatattt agcataatct ccatttttat ctataagttc tctatgagag ccctgttcaa
    12421 atatagtctt attctttata aaaataattt gatcagcagt tgatgcacaa gtcattctat
    12481 gagaaatata aataacaagt ttatctgcag ctaaattctt gaatttgctt aataaatcat
    12541 cttcagatat tgggtctaat gcagatgacg gctcgtccag cattattact ggtgtttctc
    12601 taaacaaagc tctagccaat gcaagctttt gattttctcc tcctgaaaga attactccgt
    12661 cttcataaaa aatcttatca aggtttgtat gtatcccttt aggtaaagtt ttaatcttac
    12721 cacctaatcc aacttcttga agatatgaag aaatcttaaa ttcattacta tcattggatt
    12781 tattcaaagc tacattttca gcaattgtaa aagaaaagta tttaaaatct tgaaaaacta
    12841 tgctaaatat atttctataa tccatataat ctatttgatt tattggaata ttatttaagt
    12901 atatggttcc tgaagttggt tcatataatc cgcttattaa attaattagg gtactctttc
    12961 ctgctccatt ttctccaaca ataacgtaga ctttaccata ttcaaaaaca taatttatat
    13021 tttccagaac atatttatct gttcctggat aagcaaaata tacattctct aaacgtattg
    13081 ttaaatcatt accttttaat acactctcta ttgtttttat ttctccattt tttaataaga
    13141 tatcattatt tctcttaata tcactttcca gctccataaa ttctctaaaa gattctatat
    13201 actttccatt tatatttaat tgagttataa atcctacaaa gtttaatata gcacttgaaa
    13261 gctgacttgt agcagtaaaa tattgagaaa attgtcccac agttatatta ccaataaaag
    13321 ttttccatgc taaatataaa taaatgccgt tactttttaa attttctcct attatactta
    13381 ctattttaat attcatactc gtattgtata attttttcaa tctaattctt aaaaagctta
    13441 gagtagtcaa atatttgttc ataatccagt caacaaatcc aaacatcttc atttcttttg
    13501 cagaactact ctcccccata atccttatat aatagttaag aatacgtgtt aaatatatat
    13561 tttcatctct ttgtgagatt ttccaattct ccgattttaa atttagataa acagaaaaaa
    13621 atgaaaaaag tattataacg aaaattaaat taatatctgc agagattatt ataatggtag
    13681 tagatataat taatactata cttgataaag aatgaaataa actatttatt aaattgatac
    13741 ctattccttt atagattata tcttgggcta aagctaattt ttgatgagtt tccggcaagt
    13801 ctaattgtgc catatctaat gatacgacct ttttattaat aattaataaa aaatgcattg
    13861 cataaagatg cccattaaga ttcacatatt tttcagtcaa aatcgcagta attagtgatg
    13921 ctgtcaattg aattaaaata taaattaaaa tatagtttac tatttcacta aaattcttac
    13981 ggttaattaa tccatcaatt attagtttcg gtattattat atatattaat ggaattaaac
    14041 cttctataat tgaaattatt ccttgggata tgacatagga tttactaaac tcaaaaacat
    14101 atttgaaaaa ataaaaatta ttctttatat tgtttttata atttttttta gagtccatat
    14161 aagctccttt tttcttgata atataaacat caaggggctg gtatttaaat aaataacaat
    14221 ttaagtacca gccaactgat atttaactaa tttgcagtcc cgcagccgca gccacagccc
    14281 attccatttg gagggcaggt attaccgcca ccttcaccag aacaattaca accacaccca
    14341 tagccaggat aatccccgcc attccctggc ggaaagcctg tacagccaca gccataacca
    14401 ttttgacaac cacagccaca acccatacca ggattatcat taccattaaa gcatgaattt
    14461 ctaactatct ccatggaata tcacctcttt tctaaaatat attattaata tattttaagc
    14521 ccatgaatta ataaaatatt gtctgaaata aactagatta ctatattcat actcatttat
    14581 atctttaaaa tctcaatatt ttaatatacg attttcatat taatacctac taatcaagtc
    14641 cttaaaaatt acatactttg aattatgctt tagtcccaca accacaacca catccacatc
    14701 cgcagccaca tcctgcccca ctagccataa tcgatatacc attatttttt tcatctctaa
    14761 ttatatccac attatcacct ccacattaat atattattta ttattctata ttcatgtgct
    14821 taaaaccaaa gaatttaagc atattaccta taagtactta ttaaattttc taattcgctt
    14881 tcttcaattt tgggatcaca aaaatatttt tccccatgaa cacgtccatt tacacatcta
    14941 ccgccaaaac aaattggtaa taccctacac tccttgcact catcttgcaa gtcaacctta
    15001 ttactaatcc acctagaatg tttatcttca gctaaattca ttgaaccatc ttctgatatg
    15061 tatccaattt tattaagatc atcatctaat gataaggtac attttaaaat cgaaccatca
    15121 taatcaatta ttaaattatt gtgtttagaa gcataacata ccctactata aggtcgtgtc
    15181 ataacatcca ccacatcatt gtttatgttt agctgtttta ttactttagc aatatttaca
    15241 gaggatttag caacatctaa tttatctagt atatctaatt tatcatcatt acagccacca
    15301 agtcttttta taccttcata atatatacta aacctattat caaaattttc ttttatatat
    15361 ttataaaatt cttctgcccg taaaaatact tcatcattaa aattagtgcg tattgttact
    15421 ttaaaattat aatccgtgct agccatatat ttcaaatttt ccattatctt gtcccaacta
    15481 cctctaccat caacactagc cctatactta tcatgagttt ctttaaatcc atctactgtt
    15541 atctgataat atctacagtt taaagaagaa agaacttcaa acctttctgg atatatcaac
    15601 gcaccatttg ttgttgcgct tgcactataa ggaattccgt actcttcaca taatgatttt
    15661 acttttccta aaaaagctac aactttatca tattctacaa atggttctcc gccaaaaagt
    15721 gatatagcaa cacctgtata taatttattt cttagagaat tatctatata cttcaatata
    15781 ctgctataaa ggtcttctgt catagcttta ttctcatgtt cttcatagca gtaaatacat
    15841 ctgagattac attgccttgt tacaattaaa gtcagtacta aggtagtatc tcttacaaca
    15901 tccctttcat aacaatacct agcaaattca tattctcttc tatctttagg aactagaatc
    15961 cctttttcat aaaaagtcaa tatcatctca tcagagttat cataaataat tgtatctttt
    16021 tccattatct ctctcaattt tctaacattt atttctgaat aaatgtaaat tattgcacca
    16081 gaaagagaat tataaaccaa atatgtaccg tcctctttag gtataaaaaa attataatag
    16141 ctttttttca atacatttgg ttttatcata agcttttcca ttaaatcatc tcctcatata
    16201 atatgatcat ccccaactta taagccacct aagtatattt aattctttac atagctatat
    16261 ttcccctatc ctcccctcta gttttaatat cattaaaaaa ctgctaatgg aaaagaagta
    16321 attaaatttc atccattaat cacattactg tcaaatttat tattgttaga attaagataa
    16381 taagccatat atcctttttg cattgcttat atcatactct ttcccttata ataataatag
    16441 caggtacgat tttaaatagt taaaacttgg agtaagtggt ctttttttgg gctgaacggt
    16501 ttttaggagg atagttatgt tcaaaattgg gatttgtgag gataataaaa ttttttcatt
    16561 agaattgaag tcaattattg aaagaaaatt tactcagtac aatttagaat ctaaaataaa
    16621 ttgcttttat tcaggagaag acactcttga aggtattcta aatgcaaagg aaaggtatga
    16681 tattatattt tttgatatag agctaccaaa attaaatgga atagaagtag cacgaaaaat
    16741 tagggaatta tatgatgata ccatatttat atttatcaca tatctagatg aaaaggtata
    16801 tgaagcttta gatttaacaa tttttcattt tattagaaaa agtcattttc aaaaggaaat
    16861 taatttaatt cttgattcat taataaaaaa acttgactat ttaactgaaa aatatgcctt
    16921 tcccataggg gaagataatg tttattttag actttatgat attttatatc ttgaaatatt
    16981 aaatagacaa ttaattatac atactaaaga aaatgtatat acttcaacct atcgttcctt
    17041 aaaagatatt tcatttgatt taacaaaaaa acatttttat gaagtatatc gagggattat
    17101 ggttaatctc aatcatgtaa aggattttat agataataaa attatccttt ctaatggaga
    17161 tatagtttat atagcaagaa gaagactcaa tggatttaaa gaagaattct ataaatatat
    17221 ttcatccaag agagagggat aataatgtca cgtcttaata ttatattaaa ctttttattc
    17281 tgtataatag atgctatgat ctttataaat ctgttatata tttttaaaga taataaaagc
    17341 gaatacaaat tcactgataa tatctctata atagcaggaa tatccattgc aatattcttc
    17401 tttactcagt tgggaattat tccttacact aaggtaatcc ttatttcaat agttctgttt
    17461 tttataacat ttttatatga acttagatta tatcaacaac ttttaattgt tatattatat
    17521 tattttatat taattatatc agaattatta attacattat tagcttcaaa tattttaagt
    17581 gtaggtgtag atagtattgg aaaacacaac tattctttct tttttttagg aacaattagt
    17641 aagttcttat ctatattaat tctttcgtta gttaaaagaa gatttgccaa taggaaaatc
    17701 atacttccta taattctaaa ttatattatt atattgatat tactaatatc tactatttca
    17761 atggttttat tgttttattc taatttaaat tcgcattcta aaagtattca atttatttta
    17821 tttcttgttt gtttatttac cttatttact agcataggca tcttgataat ttatttttat
    17881 gcaaacgatt tttatattga tttacaaaaa gaaactacta aaaaaattta tgataagtca
    17941 tacgaaaaat ttatagctaa tgctgaattg agaaatgact ctttagctaa aatatggcat
    18001 gatataggaa accatattaa agtcttagaa aaaatgaaca aaatagaaaa taatactgat
    18061 attgaatata ttaattcaat aaaaaataga cttaaatcaa ttcccaatac cataaatact
    18121 ggaaataaat taatagatat aatattgaat gataagtatt cagaaggtat tatgaaaggt
    18181 atagactttg atataaaagc aatagctcct cctaaaatag acattgatga tatggactta
    18241 agttctatat tgtttaatac tatagataat gcaatagaag cttgtctaaa ttatagtggg
    18301 aagaataaat atatttattt agagttatat ccagatggaa actttttatg ttataaaatt
    18361 aaaaacacat atactcctat taaaaatacc tctgttaaaa atttttactt taacaaaaaa
    18421 caatatattt cttctggata tgggcttagt ataataaaag atattgtaaa taagtatgat
    18481 ggctacatgg atataaataa agatgataaa gagtattcag ttactattgt tcttcattta
    18541 aataaccaat cagtccaaga aataaccact tagtccaaat ttaaactgtc tttagttttg
    18601 tttgttataa taattataaa atttggtaca aaataaagga ggtatttatg aaggattttg
    18661 taaaagattt ttttacaaaa agattagaat taaattttat taatatttta atgatagctt
    18721 tacttgttta tgtctataat ctttctggaa acaaatattt tttagtctac ctttttttct
    18781 ttagacttat tgcattatgt atagattatc tgacagatta tttaaaaaat taaatacatc
    18841 ctagcctaaa attttcttaa tcaaaatttt tcttgaaaaa tcattcacct tatctttaga
    18901 tttttatata ttttaatttt aaagataaat aaacctttta taataaacag acagtggctt
    18961 tatgtaaagc cactgtctgt ttattataaa ttacaaatca taatcgtccc aataaaaatc
    19021 aaattcatct cccatattat ctatataata tttataatca cgatcttcat atgcccatat
    19081 agattctcta cccctatctc cttcataatt tctatatgga ccaccattat aataaaacct
    19141 cttaagatta gtatcatata atttgcgtgc tatcccaatc actatacata gtagcagtac
    19201 ccttagcgta aatatagcaa ttaatatagt agcaatatta gaaacagtag catttttttc
    19261 aattccatta tcatcaaata gctactatca atgattaatt tacaataagt ataacagata
    19321 taattgattt attgctaaag cttggattaa attgaatggg atacttaata atattaaaca
    19381 ataaatattc agaaagaact atgaaaaata tagactttga tataaaaaca ataattcttc
    19441 ttgaaataga cattgataat atggacttaa gctctatatt gtttaatact atagataatg
    19501 caatagaagc ttatctaaat tatagtggga aataaatata tttatttaga gttatatcca
    19561 gatggaaact tcttatgtta taagattaaa aatagttatg ctcctattaa gaatactcct
    19621 gttaaaaatt tttattttaa caaaaacatt atatttcttc tggatatggt cttagtataa
    19681 taaaaggtat tataaataag tatggtgcta catggattat aaccttgcta tatttctttt
    19741 gaattaaagt tcttaatttc tttcaacaat cctagtttag gtttttatat cttatttatt
    19801 aaatattaaa tagtttcaaa atgaattcta tttttttatt atccatcaac agttaacaaa
    19861 agtaaaatga tacaattcac agcaaatatc agcaataata aggactcaat tttgttcatt
    19921 tgtgaatatg ttacatatat tttccctatt attattgata taagaaaaat aattaaagaa
    19981 aaacctgttt ttttattttt ttcattaata tttcacccct ctcatactaa tatataagtt
    20041 tcattgcttg tctactccta ataaaggaaa tgaaccctta aatcctttgc tacttcatcc
    20101 aataatcata gttcctttaa tagtttcaca attgttatta agtttattgc taatagtaat
    20161 ttatttagtg tatacaaacc cataattcca atgacttact tgtttgtgga ataatattat
    20221 atatcttctt tatttaagat ttcttacatt ttatagggac acatttcttt ttctatgttt
    20281 aagacctatt tgaatattta aatttaatac tattgtgatt tatattttca tatcatgtaa
    20341 ttcataaatc ccattctcta ttatttatat ccacaactct atttccatct tatatattaa
    20401 attcttgaaa tcataatatc cttatatttg taaaaattat ttttatatta taattatata
    20461 acagaggcaa aaaaagaact ccaatgagtt cttagtatta tctagctgct tttaactttg
    20521 ctatgtcgtt ccaattcttt gatgtgacta tttccatcgt ataaaattcc tctgctaatt
    20581 tatctatttt atcattaatt tcatatctaa attcagttag ttcagctgtt tgctcaatta
    20641 tagcttttat atctctcttg atttcttctt ggtctcttct aatttgttct tgatctcttc
    20701 taatttcctc ttgac
//
