LOCUS       NZ_FPAT01000002        10393 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Actinopolyspora righensis strain DSM 45501, whole genome shotgun
            sequence.
ACCESSION   NZ_FPAT01000002
VERSION     NZ_FPAT01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinopolyspora righensis
  ORGANISM  Actinopolyspora righensis
            Bacteria; Actinomycetota; Actinomycetes; Actinopolysporales;
            Actinopolysporaceae; Actinopolyspora; Actinopolyspora alba group.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900116555.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:37:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,474
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,334
            CDSs (with protein)               :: 4,334
            Genes (RNA)                       :: 67
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 27 of 73
            Pseudo Genes (incomplete)         :: 61 of 73
            Pseudo Genes (internal stop)      :: 12 of 73
            Pseudo Genes (multiple problems)  :: 20 of 73
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:10:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 96060
            Orig. end                         :: 106453
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FPAT01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10393
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10393
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10393
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5393](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5393
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            526..1302
                     /locus_tag="BM394_RS03950"
                     /old_locus_tag="SAMN04487904_102100"
     CDS             526..1302
                     /EC_number="2.1.1.-"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1292: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973030.1"
                     /locus_tag="BM394_RS03950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487904_102100"
                     /product="SAM-dependent methyltransferase"
                     /protein_id="WP_245779952.1"
                     /transl_table=11
                     /translation="MQRPNAARMFDYYLGGTANFAADRQAVDRLVADVPEARVYARATR
                     AFLGRLIRFLSHRGIDQFLDLGSGMPTVGNVHEIARRCRSDARVAYVDVEPEVVAHARQ
                     LLRGVDNVTVTQADIREPETVLSAPEVTELLDFTRPVAVLAVSILPYVPDADDPASLVA
                     AYRDNCVPGSYLAVSHSTPLTMTEEQVRHSESAYQGTRTPLAVREIERIRELLPGYSLV
                     EPGLVPLPQWRPEPDINAQQDATASANAVGALGYLP"
     misc_feature    542..557
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.84"
                     /tool="antismash"
     misc_feature    complement(543..556)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.65"
                     /tool="antismash"
     misc_feature    complement(550..566)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 19.23"
                     /tool="antismash"
     misc_feature    complement(1704..1717)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.76"
                     /tool="antismash"
     gene            1745..2497
                     /locus_tag="BM394_RS03955"
                     /old_locus_tag="SAMN04487904_102101"
     CDS             1745..2497
                     /EC_number="3.4.21.-"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973031.1"
                     /locus_tag="BM394_RS03955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487904_102101"
                     /product="trypsin-like serine protease"
                     /protein_id="WP_217643342.1"
                     /transl_table=11
                     /translation="MSLARIFTRTAAAALIAVGMAAPAQAAVSAPVANDDSGPETMIID
                     GGYADDAPWAARMFADGRQACSASVIGPKWVLTAQHCVQGANQVSFRVGSLDQTQGREV
                     FAVPGGVHVHPSADLALVNVGSSVDVEYAPIGAPGAAEVGETVQTYGWGATCTDRPEIE
                     CQSQWLKVADVTVTDTSCSDYRGGTAICAYRGDGIPAGGDSGGPMFATAADGDYVQVGV
                     ASTSDRSSRTAYTNVTRYSSWIGSLAGV"
     gene            2970..3488
                     /gene="ectA"
                     /locus_tag="BM394_RS03960"
                     /old_locus_tag="SAMN04487904_102102"
     CDS             2970..3488
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973032.1"
                     /locus_tag="BM394_RS03960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487904_102102"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_342752637.1"
                     /transl_table=11
                     /translation="MEIDTPAVADGPELYRITRDSGVLDVNSPYAYLLWCRDFATTSLV
                     ARSHDGVVGFVTGYVRPDAGDTLVVWQIGVDAALRGHGVASRLLERLLDTASRDGIRYL
                     ETTITADNAASINLFSALARDRGAPLEVSPLFPAELFPDSHIGEDLYRIGPFAADGATA
                     HGTLAASVS"
     gene            3633..4880
                     /gene="ectB"
                     /locus_tag="BM394_RS03965"
                     /old_locus_tag="SAMN04487904_102103"
     CDS             3633..4880
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (23-344). E-value: 2.8e-68.
                     Score: 222.4. Matches aSDomain:
                     nrpspksdomains_BM394_RS03965_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947388.1"
                     /locus_tag="BM394_RS03965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487904_102103"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_092974006.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.1e-69, bitscore:
                     230.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDVFATQESEVRSYSRTWPATFDRALGSWLYDESGNAYLDFFAG
                     AGALNYGHNNPLLKRKLIEYIERDGIHHGLDQATVARGEFLRTIDEKLFRPRGLNYKVQ
                     FPGPTGTNSVEAALKLARKITGRESIISFTNAFHGMTLGSLSVTGNSMKRGGAGIPLVH
                     ATPMPYDNYFDGQVDDFLYFESLLRDSGSGLNAPAAVIVETLQGEGGINDTRAEWLRGL
                     SELCGRHGILLIVDDVQMGCGRTGPFFSFEHAGIEPDIVCLSKSIGGYGSPLALTLIKP
                     EHDVWEPGEHNGTFRGNNPALVTATEALRQYWSDDELERATLAKGERVGEALLELCADH
                     PGLLSKGRGLARGLGFAEPEMAGKVSKAAFDRGMILETSGPSDEVVKVMPPLTITEDEL
                     ERGIRIIRDSVRSVLA"
     aSDomain        3702..4664
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM394_RS03965_Aminotran_3.1"
                     /evalue="2.80E-68"
                     /label="BM394_RS03965_Aminotran_3.1"
                     /locus_tag="BM394_RS03965"
                     /protein_end="344"
                     /protein_start="23"
                     /score="222.4"
                     /tool="antismash"
                     /translation="DRALGSWLYDESGNAYLDFFAGAGALNYGHNNPLLKRKLIEYIER
                     DGIHHGLDQATVARGEFLRTIDEKLFRPRGLNYKVQFPGPTGTNSVEAALKLARKITGR
                     ESIISFTNAFHGMTLGSLSVTGNSMKRGGAGIPLVHATPMPYDNYFDGQVDDFLYFESL
                     LRDSGSGLNAPAAVIVETLQGEGGINDTRAEWLRGLSELCGRHGILLIVDDVQMGCGRT
                     GPFFSFEHAGIEPDIVCLSKSIGGYGSPLALTLIKPEHDVWEPGEHNGTFRGNNPALVT
                     ATEALRQYWSDDELERATLAKGERVGEALLELCADHPGLL"
     gene            5001..5393
                     /locus_tag="BM394_RS03970"
                     /old_locus_tag="SAMN04487904_102104"
     CDS             5001..5393
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973034.1"
                     /locus_tag="BM394_RS03970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487904_102104"
                     /product="ectoine synthase"
                     /protein_id="WP_092974009.1"
                     /sec_met_domain="ectoine_synt (E-value: 6.2e-55, bitscore:
                     179.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTVRDVENTESDIRTENWRSKRIILAKEKAGFSVHETTLYAG
                     TVNEFWYANHIEAVFVFEGEGEITDHATGETHSLGPGSVYLLDDHDKHQVRPRTDMRTV
                     CVFNPPVTGREVHDENGVYPLVTEEE"
     gene            complement(5430..6464)
                     /locus_tag="BM394_RS03975"
                     /old_locus_tag="SAMN04487904_102105"
     CDS             complement(5430..6464)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873074.1"
                     /locus_tag="BM394_RS03975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487904_102105"
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="WP_245779954.1"
                     /transl_table=11
                     /translation="MSTSSTVVDRAVGCLLGAALGDSLGVPVEFEEHGDIRARYGPDGV
                     TEPPPQALLGDAAQMLLFTGEGYLHAWATGNHGGRWRPVEATGAAYRRWLLTQQEDKPH
                     YGATGLLAEPELYVNRSPGLTSLRALQEDLLGTPEEPINSSKGCGAVDRAAGAGFAPTV
                     EMSYTLGCQFGALTHGAPGGWAAAGAMAALIHLLAVQGRRIPQAVDQAAGRVLREDGET
                     ATGLADAATLARIDVRTAYVQRLGQGWVGPEALAIGAYCALALPKREQFRDALWLAANH
                     SGHSDTTAAITGAILGARHGTAALPRDWLNRLELADVIERIGHDLGASCVGEKFNDRRY
                     LTLG"
     gene            6775..9675
                     /locus_tag="BM394_RS03980"
                     /old_locus_tag="SAMN04487904_102106"
     CDS             6775..9675
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947384.1"
                     /locus_tag="BM394_RS03980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487904_102106"
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_092974012.1"
                     /transl_table=11
                     /translation="MPPHDNLAEHPKSGYEFGLLGPLEVRHDGRLQPLGNSAMRSALAC
                     LLLEPNQVVSMDRLIDTLWGEQPPPTARTIIHGYVSRIRKLFTPEAGTADPENPALVTR
                     APGYLLQVDSDRIDAHRARALVNQAGNLEPRERSRTLARALTLWRGPVLSDISSERLHR
                     MVAPNLDELRMVALEERIEADLELGEHHGVVVELSELVEQYPLRERLTGQLMLAYYRSG
                     QRARAQSCYHTLRELLADELGIDPGPELRSLYERLLRDDETLAAPNRAVAGSITPASDV
                     TPIQPAELPPPVAGFVGRATESAALDRMLSEQDSDSASVLAAITGTAGIGKSALAVTWA
                     HRVAHRFPDGQLYASLRGFDYERSPLSPGEVLTGFLKSLGIADDAIPIDLNERVALYRS
                     QLARRRVLILLDDARDADQVRPLLPSSDGSLVLVTSRKRLDGLVVRSGARALPLESLPT
                     TAAVELLDRAGVPGRSRSEPQAARKLADLCGGLPLALRIAAARLAANPGRAVDDLVGEL
                     TDEHNRLTGLDIDDTDISVRRAFDISYRNLHPSQAWTFRLLGLVPGHTFTAHAVAALCG
                     TDSETARRRLRALTLAHLVSEPRPDRFAMHDLLRVYARELVFAATDETGEESETALRGL
                     LEHYLTAADHARRFLRPPRDELDFSATEWEVPDITGRAEALEWFDAEWPNLVTAIDTAA
                     DRRWHRMAWWLVRLQFNYLMVRCPWEEWVTIYTRGLESARHIGDDTGTVLMLAGLGVVH
                     SRSGSPEVALKYYAESQRVATKLSERGELAMTQVNMASALFRLGRYSEARQHCEDALRT
                     YRSLGDRYREAGALNNLAQVEQVIGNRAEALAYLHEAEKRYREADDLEILAMVLNNCGE
                     VSVELDRIDSARRYYGEALEVAQRCGSTMRQAAAYLGLGDVASLREDHQVARRRWETAL
                     SIFESGGSPRAAEPRERLRLGAAATEAPEVRRCSGV"
     misc_feature    8041..8043
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 cctggcaggt ggacgagggg caccggctgg tgctggtggt cgacaccgtg gacgctcgtt
       61 accgcgacga gagctccggt ggcgaggtga cgttcggttc ctcggccgaa cagccggcca
      121 aattgcgaat tccggtgggt tgacaccgtt tccgctccca cgagcgcctg ctgttcggct
      181 ctccggtggt gcccgtgtcg gatggtgcgg gcaccactgt gaccggccga aaaccgatcc
      241 gaaaaaccga ctgaactgtt cggatccgtt cggttgtgct atcaggattc gatcgaatac
      301 gaatagtgtc caaaaagtag cgcaacgaag tttttctcgc tgttcgcggt atgttttcgc
      361 cacgggcggg aacactgctc cacaatcgcc ctcttcggct gtaccgtggc gtatatgacg
      421 gatgttcgtc caccggacgc gttacagcgc gaaggtgccg atgatgagcc accgagaaga
      481 cccttaaggc gcatatcggg agatctgcag gtcaggggcg tggacatgca gcgccccaat
      541 gccgcacgca tgttcgatta ttacctcggg ggtacagcca atttcgcggc ggacaggcag
      601 gccgtcgacc ggctggttgc cgatgtgccc gaggcccggg tttacgcacg tgcgacgcgg
      661 gcttttctcg gacgattgat ccgttttctg agccatcgcg gaatcgatca gtttctggat
      721 ctgggatcgg gaatgccgac cgtcgggaat gtgcacgaaa tagcccgccg gtgccgttcc
      781 gacgcacgag tcgcctatgt ggatgtcgag cccgaggtcg tggcgcacgc caggcaactg
      841 ctgcgcggag tcgacaatgt gaccgtcact caggccgaca tccgggagcc ggagacggtg
      901 ctctcggcac cggaggtcac cgagctgctc gacttcaccc gaccggtcgc ggtgctggcc
      961 gtgtcgatcc tgccctacgt tccggacgcc gacgaccccg cctcgctcgt ggccgcctac
     1021 cgggacaact gcgtgccggg cagctacctg gccgtctcgc acagcacgcc gctgacgatg
     1081 accgaggagc aggtgcgcca cagcgaatcg gcctaccagg gaacccgcac gccgctggcc
     1141 gtgcgtgaga tcgagcggat ccgcgagctg cttcccggct actcgctggt cgagcccgga
     1201 ctggtcccgc tgccgcagtg gcggccggag ccggacatca acgcccagca ggacgccacc
     1261 gcctccgcca acgcggtagg agcgctcggc taccttcctt gaacttgtac tcccgttgtg
     1321 tagctggtcg ggtagccgtc acgtgagtcg gcgccttgcg gcgttgggcc ggctcttgag
     1381 tagcgaccta cgcggcgagc ggcccggcct cgcgatgcat ccgactcacg caccggggac
     1441 gagcccgctg ccgcgctcat ggtcgcgtct cgacgggctg cccggcgatt tcgtgagaga
     1501 tcgacgccgc gccgagcggg cgcccgaggc acgagcgggg gacacgtgcg cggttcgtgg
     1561 gggcagcccg gcgccgggtc cggttcggaa cggtcgaggg cagcccggct tcgagtggtt
     1621 accaagccac gtaccgcgtt ggcgattagt gaccaattta ttgattttgc tactcgatcg
     1681 ggtgactttg ggatgcgtcc tgatcacgca gcgacacagc tgctgtccca aaaggagccc
     1741 cagcttgtca ctagcaagaa tcttcacccg gactgccgcc gccgcgctga tcgcggtcgg
     1801 catggccgct ccggcccagg cagccgtctc cgctccggtc gccaacgacg actccggccc
     1861 ggagacgatg atcatcgacg gtggttacgc cgacgacgcc ccgtgggcgg cgcggatgtt
     1921 cgccgacggc aggcaggcgt gcagcgcctc ggtgatcggt ccgaagtggg tactcaccgc
     1981 acagcactgt gtccagggcg ccaatcaggt cagcttccga gtgggcagcc tcgaccagac
     2041 ccagggccgt gaggtgttcg cggtgccggg cggggtgcac gtccatccca gcgcggacct
     2101 ggcactggtc aatgtgggca gttcggtcga cgtcgagtac gccccgatcg gcgccccggg
     2161 tgccgccgag gtcggcgaga ccgtgcagac ctacggctgg ggtgccacct gcaccgaccg
     2221 tcccgagatc gaatgccagt cccagtggtt gaaggtcgcc gacgtgaccg tgaccgacac
     2281 ctcctgctcc gactaccgcg gcggtaccgc gatctgcgcc taccgcggtg acggcatccc
     2341 ggcgggcggt gactccggcg gcccgatgtt cgccaccgcc gccgacggtg actacgtgca
     2401 ggtgggagtg gcttccacca gcgaccgctc ctcgcgtacc gcctacacca acgtgactcg
     2461 ctacagcagc tggatcggtt ccctggcggg cgtgtgacgc ggcttcctcc cgtcgtggct
     2521 acacccggtt tccccgtcgc cgttcacggc ggcggggaaa tgtgtctgga agcacacgtg
     2581 gggcgacttc tttccgtgtc gcttcgggtt cgggcattga ccgctgccga agtctcggtg
     2641 catcgtgacc gacgaaatcg atcacagggt gagatcgtga tcgtattcgg cgcgaaaaat
     2701 agatcgtttc gttgttttcg cagttcatgg cgctgatatt ggcctcttca gaggtggagt
     2761 gaatcgctgc tagcgattct ttctcggcgt cgcaagaaat ttgttgtgct tgtcacgggg
     2821 tattttgctt cgcgcctggt ggaggaaatg tcgatctttt ctcgcctggc gatttcaccg
     2881 gtgcgtggtt agtttcgtga gacatgaccg gcgaaaaacc tggccatcgt ggcgcacggt
     2941 ccgatggcgt gagtgggtca gccgggcggg tggagatcga cactccggcc gtcgcggacg
     3001 gcccggagct gtaccggatc acgcgtgatt cgggggtgct ggacgtcaac tccccctacg
     3061 cctacctgct gtggtgtcgc gatttcgcga ccacctcgtt ggtggcgcgt tcgcacgacg
     3121 gagtggtggg cttcgtgacc ggatacgtcc ggcccgacgc cggggacacc ctcgtggtgt
     3181 ggcagatcgg cgtcgacgcc gcgctgcgcg gccacggcgt ggcgagtcgc ctgctggaac
     3241 ggctgctcga cacggcctcg cgggacggta tccgctacct ggaaaccacc atcaccgccg
     3301 acaacgcggc ctcgatcaac ctcttctccg cgctggcgcg cgaccgcggc gctccgctcg
     3361 aggtgagccc gctgttcccg gccgagctgt tcccggactc acacatcggc gaggacctct
     3421 accgcatcgg accgttcgcc gctgacggtg ccacggcaca cggcactctc gcggcgtccg
     3481 tctcgtgagg ctccacgccc cacaaacccg acccggttcg cggtgaaacc cgagccgcga
     3541 accatccagc cgtgtcaccg cacggcgcga cacgtaaccg gcccggcggg cgaggcgtca
     3601 cgagcgcttc gaaccggacc gaacggagga tcgtgaaaga cgtcttcgca acccaggaat
     3661 cagaagtccg cagctacagc cgaacctggc ccgcgacctt cgaccgcgcg ctgggcagct
     3721 ggctctacga cgagagcggg aacgcctacc tcgacttctt cgccggggcg ggcgcgctca
     3781 actacgggca caacaacccg ctgctcaagc gcaaactgat cgagtacatc gagcgcgacg
     3841 ggatacacca cggcctcgac caggccacgg tcgccagggg cgagttcctg cgcaccatcg
     3901 acgagaagct gttccgaccg cgtgggctca actacaaggt gcagttcccc ggccctactg
     3961 gcaccaactc ggtcgaggcc gcgctgaagc tggcacgcaa gatcaccgga cgcgagtcga
     4021 tcatcagctt caccaacgcc ttccacggca tgacgctggg ctcgctctcg gtcaccggca
     4081 actcgatgaa acgaggcggg gcgggcatcc cgctggtgca cgccacgccg atgccctacg
     4141 acaactactt cgacggccag gtcgacgact tcctctactt cgagtcgctg ttgcgggaca
     4201 gcggcagtgg cctgaacgct cccgccgcgg tgatcgtgga aaccctgcag ggcgagggcg
     4261 gtatcaacga cacccgcgcc gagtggctgc gcggactgtc cgagctgtgc gggcggcacg
     4321 gcatcctgct catcgtcgac gacgtgcaga tgggctgtgg caggaccggc ccgttcttca
     4381 gcttcgagca cgcgggcatc gagccggaca tcgtctgcct gtccaagtcc atcggtggtt
     4441 acgggagtcc gctggcgctg accctgatca agccggagca cgacgtctgg gaacccggcg
     4501 agcacaacgg caccttccgt ggcaacaacc ccgccctggt cacggcgacc gaggcgctgc
     4561 ggcagtactg gagcgacgac gaactggaac gcgccaccct cgccaagggc gagcgcgtcg
     4621 gcgaggcgct gctggagctc tgcgccgacc accccgggct gctgtccaag ggacgtggcc
     4681 tggcacgggg cctcggtttc gccgagcccg agatggcggg caaggtttcc aaggccgcct
     4741 tcgatcgggg gatgatcctg gagacctccg gccccagcga cgaggtggtc aaggtgatgc
     4801 ccccgctgac gatcaccgag gacgagctgg agcgagggat ccggatcatt cgggactccg
     4861 tgcgctcggt actggcctga agcactcaca ggcgccgaag cgcccgtcgc gagccggggc
     4921 gtgcgcgaac acgctcccgc gcacggcgcc cgccgcgtcc ggcccctgaa acgtccaccc
     4981 cgaaacaccg aggagaagac ttgatcgttc gtaccgtccg ggacgtcgag aacaccgaat
     5041 ccgacatcag gaccgagaac tggcgcagca aacggatcat cctcgccaag gagaaggccg
     5101 ggttctcggt gcacgagacg acgctgtacg cgggcacggt caatgagttc tggtacgcca
     5161 accacatcga ggccgtgttc gtgttcgagg gcgagggcga gatcaccgat cacgccaccg
     5221 gtgagacgca ttccctcggg ccaggctcgg tgtacctgct cgacgaccac gacaagcacc
     5281 aggtgcgccc caggaccgac atgcgcacgg tctgcgtgtt caacccgccg gtgaccggca
     5341 gggaggtgca cgacgagaac ggggtctacc ccctggtgac cgaggaggag tgaaccgcga
     5401 gggcacctga cgaccttccc gggctgacct caacccaggg tcaggtatcg ccggtcgttg
     5461 aacttctcgc ccacgcagga cgcgccgagg tcgtgtccga tgcgctctat cacgtcggcc
     5521 agctccagcc ggttgagcca gtctcgcggc agtgcggcgg tgccgtgcct ggcgcccagg
     5581 atagccccgg tgatggccgc cgtggtgtcg ctgtgtcccg agtggttggc ggccagccac
     5641 agcgcgtcgc ggaactgttc gcgcttgggc agggccagcg cgcagtaggc cccgatggcc
     5701 aacgcctccg ggccgaccca accctgtccc agccgctgga cgtacgccgt gcgcacgtcg
     5761 atgcgggcga gagtcgccgc gtcggccagt cccgtggccg tttcgccgtc ctcccgcagc
     5821 actctgcccg ccgcctggtc caccgcctgc ggaattcgcc tgccctgcac cgcgagcagg
     5881 tggatcagcg cggccatggc tcccgccgcc gcccaccctc cgggggcgcc gtgggtcagc
     5941 gcgccgaact ggcagcccag tgtgtaggac atctcgacgg tgggagcgaa gccggctccg
     6001 gccgcgcggt ccaccgcgcc acaccccttg gaactgttga tcggctcctc gggagttccc
     6061 agcaggtcct cctgcagcgc gcgcaggctg gtcagcccgg gggagcggtt cacgtagagc
     6121 tccggctcgg ccaacaggcc cgtcgccccg tagtgcggtt tgtcctcctg ctgcgtcagc
     6181 agccaccgcc gataggcggc gcccgtggct tccaccgggc gccaccggcc gccgtggttg
     6241 ccggtcgccc aggcgtgcag gtatccctcg ccggtgaaca gcagcatctg cgcggcgtcg
     6301 ccgagtagcg cctgcggtgg tggttcggtt actccgtcgg ggccgtagcg cgcacggatg
     6361 tcaccgtgct cctcgaactc gaccggaact ccgagggagt cgcccagcgc cgcaccgagc
     6421 aagcacccca cggcacgatc caccaccgtg gacgacgtcg acacccggga tacctccaca
     6481 gtggtcttgc gcgcgttctg atcctcaccc gactctactg gcccgccaca gccacttggc
     6541 agcgatctgt gcaattgcac ggcggagttt gctcacagat cgtgacattc gtcgaataat
     6601 aaaagttgat cttagtctgt tggggtgaag ccaggtgaat ccgaacacgt ttgtcggttt
     6661 tagtggttaa cgtggatcgg tttcgggacg tttgtgcgta gggcggcacc ctgcggccgc
     6721 tgtcacggaa ccgggaaaga tgtacgcgaa cgtgggatcg agcttcgggg ggatatgcca
     6781 ccgcacgaca acctggccga gcacccgaag agcggctacg agttcggact actcgggccg
     6841 ctcgaagtgc gtcacgacgg gcgactgcag ccgttgggca actcggcgat gcgcagtgcg
     6901 ttggcctgct tgctgctcga accgaaccag gtcgtgtcga tggacagatt gatcgacact
     6961 ctttgggggg agcaaccacc gcccacggcg cggaccatca tccacggcta cgtgtcgcgt
     7021 atccgcaagc tgttcacccc cgaagcgggg acagcggatc cggagaatcc cgcactggtc
     7081 acccgtgccc ccggctacct gctccaggtg gacagcgacc ggatcgacgc gcaccgggcc
     7141 cgagccctgg tcaaccaagc cggaaacctc gaaccccggg aacgttcccg gacgttggcc
     7201 cgcgcgttga cgctgtggcg tggtccggtg ctttccgaca tctcctccga gcgactgcac
     7261 cgcatggtcg cccccaacct ggacgagctg cgcatggtgg cgctggagga gcggatcgag
     7321 gccgacctcg aactgggcga acaccacggt gtcgtggtcg agctgtccga actggtcgag
     7381 cagtacccgc tccgcgagcg gctcaccggc cagttgatgc tcgcctacta ccgatccgga
     7441 caacgggcca gggcccagtc ctgctaccac acgttgcggg aactgctggc cgacgaactc
     7501 ggcatcgatc cggggccgga actgcgctcg ctgtacgaac gactgctgcg cgacgacgag
     7561 acactggccg ccccgaaccg tgccgtggca ggctccatca cccccgccag tgacgtgacc
     7621 ccgatacaac cggcggaact acctccgccc gtggccggtt tcgtcggaag ggccaccgaa
     7681 tcggcggcgc tggaccggat gctgtccgaa caggatagcg actccgccag cgtgctcgcc
     7741 gccatcaccg gaaccgccgg aatcggcaag agcgcgttgg cggtgacctg ggcgcaccgc
     7801 gtcgcgcatc gattcccgga cggccagctc tacgcctcgc tgcgtgggtt cgactacgag
     7861 cgttcgccac tctcaccggg cgaagtgctc accggtttcc tcaagagcct ggggatcgcc
     7921 gacgacgcca taccgatcga cctgaacgaa cgggtggccc tgtaccgctc gcagctggcg
     7981 cggcgccgcg tgctcatcct gctcgacgac gccagggacg ccgaccaggt gcgtccgctg
     8041 ttacccagta gcgacggttc gctcgtgctg gtcaccagca ggaaacggct cgacggtctg
     8101 gtggtgcgca gcggggccag ggcgttgccg ctggagagcc tgcccaccac cgctgccgtg
     8161 gagctgctgg atagggccgg tgtgccgggc agatcccgct ccgagccgca ggccgcgcgg
     8221 aaactcgccg acctgtgcgg cggcctgcca ctggcgttgc gcatcgccgc cgcgcgactg
     8281 gccgccaacc cgggccgcgc ggtggacgac ctcgtcggcg agctcaccga cgagcacaac
     8341 cggctcaccg gactcgacat cgacgacacc gacatcagcg tccgtcgtgc cttcgacatc
     8401 tcctaccgca acctgcaccc cagtcaggcg tggaccttcc ggctgttggg gttggttccc
     8461 ggccacacct tcaccgcgca cgcggtggcc gcactgtgcg gcaccgactc cgagacggct
     8521 cgtcgcagac tgcgagcgct gacactggcc cacctggtca gcgagcccag accggaccgg
     8581 ttcgcgatgc acgacctgtt gcgggtctac gcgcgcgaac tcgtgttcgc cgccaccgac
     8641 gaaaccgggg aggagtcgga gaccgcgctg cgcggactgc tggagcacta cctcaccgcc
     8701 gccgaccacg ccaggaggtt cctgcgccca ccgcgtgacg agttggactt ctcggcgacc
     8761 gagtgggagg ttccggacat caccggcagg gccgaggcac tggagtggtt cgacgccgaa
     8821 tggcccaacc tggtgaccgc catcgacacg gccgccgacc ggcgatggca ccggatggcc
     8881 tggtggttgg tgcgcctgca gttcaactac ctcatggttc ggtgtccctg ggaggaatgg
     8941 gtcaccatct acaccagagg actcgagtcg gcgcgtcaca tcggcgacga caccggcacg
     9001 gtactgatgc tcgccgggct cggagtggtg cactcccgtt cgggcagccc cgaggtggcc
     9061 ctgaagtact acgcggagtc gcagcgggtc gccaccaagc tgtccgagcg cggcgagctg
     9121 gccatgacgc aggtcaacat ggccagcgcg ctgttccggt tgggacgcta ctcggaagcg
     9181 cgacagcact gtgaggacgc gctgcggacc taccggtcgt tgggggaccg gtaccgggaa
     9241 gcgggagcgc tgaacaatct ggcccaggtg gagcaggtga tcggcaaccg tgcggaggcg
     9301 ttggcatacc tgcacgaggc cgaaaagcgc taccgggaag ccgacgatct cgaaattctg
     9361 gccatggtgc tcaacaactg tggtgaagtg agtgtcgaac tggacaggat cgacagcgcg
     9421 cggcgctact acggggaggc gctcgaagtg gcccagcgct gtggctcgac catgcgccag
     9481 gccgcggcgt atctggggtt gggtgatgtc gcctcgctgc gcgaggatca ccaggtggct
     9541 cggcggcggt gggagacggc actgtccatt ttcgagtccg gtggctcacc gcgtgccgcg
     9601 gagccgcgtg aacggttgcg cctgggtgcc gccgccaccg aggcacccga ggtgcggcgc
     9661 tgttccggcg tttagtcagc gtttagcgtg ctcttagtgg tgcctgccaa cgtgtgaatg
     9721 tgcgttccgg ccggccgttg cggtgccggt cacgatcgca ccgcggcgac tctcgggggg
     9781 tcgtgagtgg ccgagaaggg gcggtgcgga gagctcgacc cgtcgagttg tccaccgccc
     9841 gcaacaaagg ggtcaggtta ttcccggcgt cttcggcacc gggaaggaca tgggggtggg
     9901 tgcggactcc gtgttctggg ggattccaca acgacacggg gtccgcgctc gtgttcccgc
     9961 ccggcccgtc ccgagggccg ggcggttcga cccaccgccg ctgcgccccg gcggcgatca
    10021 gtagtgcttt cccggatccg ggggcgtccg ggtcagccgt gctccgaagc gccctggagc
    10081 gggcgcggtg agggccgttt gggttctctg ccgtcccccg aggacgtgcc gcgcagccgc
    10141 cgaccgaccc acgggcccag gtgttcaccg atccagcgca ggtccgcacc gcgccgttcc
    10201 gaccaggagc gatcggcccg agcaggaaac ggctgccgcc agtcctcgtc cacgcgcagc
    10261 cccaggatct ccgcggcgtg cagcgccacg cggtgatgtc cctcctggga caggtgcagt
    10321 ctgtcctcgc tccaggcacg ctcatgctgt aacacccgca tcgaccagag gtccacgaca
    10381 tagcagccgt gtc
//
