LOCUS       NZ_FPAT01000001        10960 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Actinopolyspora righensis strain DSM 45501, whole genome shotgun
            sequence.
ACCESSION   NZ_FPAT01000001
VERSION     NZ_FPAT01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinopolyspora righensis
  ORGANISM  Actinopolyspora righensis
            Bacteria; Actinomycetota; Actinomycetes; Actinopolysporales;
            Actinopolysporaceae; Actinopolyspora; Actinopolyspora alba group.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900116555.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:37:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,474
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,334
            CDSs (with protein)               :: 4,334
            Genes (RNA)                       :: 67
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 27 of 73
            Pseudo Genes (incomplete)         :: 61 of 73
            Pseudo Genes (internal stop)      :: 12 of 73
            Pseudo Genes (multiple problems)  :: 20 of 73
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:10:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 174256
            Orig. end                         :: 185216
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FPAT01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10960
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="3"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10960
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10960
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5960](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5960)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            272..1132
                     /locus_tag="BM394_RS00795"
                     /old_locus_tag="SAMN04487904_101166"
     CDS             272..1132
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973321.1"
                     /locus_tag="BM394_RS00795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487904_101166"
                     /product="TatD family hydrolase"
                     /protein_id="WP_092972746.1"
                     /transl_table=11
                     /translation="MRIFDPHIHMSSRTTNDYDAMYRAGVRALVEPSFWVGQPRTSVGT
                     FVDYFDGLIGWEPFRASQYGIAHHCTIGLNPKEANDPRCRDVLEVLPRYLAKDGVVAVG
                     ELGYDAMTTEEDDAFAAQLELAAEHELPVLVHTPHRDKEAGTRRSLDVIRESGIPVERV
                     VVDHLNEVTVEAVAESGCWMGFSIYPDTKMDEDRMVRIMRGYGVERMLVNSAADWGKSD
                     PLKTSKTAHAMLAAGFEPAAVDTVFWSNPVGFYGQSGRLRTETSTLGDGDGTTFAGNSI
                     ERGKR"
     misc_feature    662..664
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1135..2298
                     /gene="eboE"
                     /locus_tag="BM394_RS00800"
                     /old_locus_tag="SAMN04487904_101167"
     CDS             1135..2298
                     /codon_start=1
                     /gene="eboE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973320.1"
                     /locus_tag="BM394_RS00800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487904_101167"
                     /product="metabolite traffic protein EboE"
                     /protein_id="WP_092973589.1"
                     /transl_table=11
                     /translation="MRFRHRDGSTVHLAYCTNVHPAEDLEGIIAQFDYYAVPIRRRLGV
                     ERLGLGLWLARDVATTLVEDDTALARLRTELLARGLEVVTLNGFPYRGFQRAVVKHAVY
                     RPDWSERARLDYTLDLAKLLSHLLPEDVTSGSVSTLPLGWGAWWSEHHHNRALAHLTEL
                     TDGLARLAAREGRSVRVGLEPEPGCVLETTDQLVSALSGTDTEHLGICLDTCHVAVAFE
                     NPHRALRRLHEAGLPVVKTQASSALRVDNPGDSTQRAELNSFVEPRFLHQTQQSGDGVR
                     RAVDDLGSALAGELSGDQPWRVHFHTPLHTDPSASLRGTHDVLGETLRSLLGDNTAHTR
                     HVEVETYTWDVLPDSGDHSASIGAGIAAELDWARRRLLELGLTEEGR"
     misc_feature    1140..1160
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 21.99"
                     /tool="antismash"
     gene            2295..3707
                     /locus_tag="BM394_RS00805"
                     /old_locus_tag="SAMN04487904_101168"
     CDS             2295..3707
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973319.1"
                     /locus_tag="BM394_RS00805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487904_101168"
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="WP_092972748.1"
                     /transl_table=11
                     /translation="MNHTAEARTPVLLLDVVGLTPRLLEHMPNLDRIARRGFRAELKTV
                     FPAVTCPVQSSLLTGRHPGDHGIVGNGWFFRDIGEVFFWRQYNGLVRGEKIWQAARERD
                     PGYRVSNVCWWYAMGMDVDETVTPRPVYHADGRKSPDCYTYPTGLRDELTDKFGTFPLF
                     NYWGPTASIESSRWIAEATGHLLSTRGSDLTMAYIPHLDYDLQRYGPDSPRAAAAAREV
                     DRALAPLLNRAEAQGVTVLALSEYGMTEVSRPIDINRALRRERLLHVHTQAGMEYLDPW
                     SSRAFAVADHQAAHIYVRDTDDLARVRDVVGALPGVDEVLDTSGKKRYGIDHERTGDLV
                     AVAEPDSWFTYYYWLDDARAPDFATHVEIHRKPGYDPAELFMDPRDRLVRLRAATAVAR
                     KKLGLRYRMNVVPLDPTPVHGSHGRLADSASHGPVLLCSDERFGRESYHATEVKDLLLE
                     LSGLREPRRTTR"
     misc_feature    3465..3467
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3732..4742
                     /locus_tag="BM394_RS00810"
                     /old_locus_tag="SAMN04487904_101169"
     CDS             3732..4742
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020512725.1"
                     /locus_tag="BM394_RS00810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487904_101169"
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="WP_092973591.1"
                     /transl_table=11
                     /translation="MPRPITLFTGQWADLPFEDVAKLASEWGYDGLEIACWGDHFEVDR
                     ALAEKDYVRRKREVLEKHDLNCWVLSNHLLGQATCDYPIDERHRNILPPEIWGDGDPEG
                     VRQRAAERMRDTARAAAEFGVDKVVGFTGSPIWYTVAMFPPTPDEMVQRGYQEFADRWN
                     PILDVFDECGVRFALEVHPTEIAYDYWTTVRTLEAVDHRSALGLNFDPSHYVWQGFDPV
                     TFLWDFQDRILHVDCKEARVRSNDGRSGVLGSHLPWGDPRRGWDFVSTGHGHVPWEDCF
                     RTLNTIGYDGPISIEWEDAGMDRLRGAPEALEFVRNLNFTPPAASFDAAFGSETQ"
     gene            complement(5001..5960)
                     /locus_tag="BM394_RS00815"
                     /old_locus_tag="SAMN04487904_101170"
     CDS             complement(5001..5960)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019854149.1"
                     /locus_tag="BM394_RS00815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487904_101170"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_092972750.1"
                     /sec_met_domain="AfsA (E-value: 1.8e-20, bitscore: 68.2,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSSIVADTVTEESREQPTVSFEQSLPRRWVHRAANTEVLVTSFG
                     ERGENDFVLGAQLPRAHALYGPVAGGHDPLLFAEAARQAGLVVGHVGFGVPYGHQFIMD
                     TLDYEVKPEALRLADTPAQIVLEIHCENVKWRGRTFAGANMHVHGYRDGVFVGRVTLPY
                     SCISPAAYARIRAGCDDFTDAADQAELPESVPPWRVGRDCPSDVLLAPTPHERTWLLRD
                     GRTHPVLFDHPVDHVPGMTALEAARQAARSEFCPDTVLPVGMRTRFHSYIELGRSCFVR
                     VHDEPIAGDTVRVTLEQEGRVAVDSTVLVRRLSDETRR"
     misc_feature    complement(5787..5789)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(5918..5933)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     gene            6135..7163
                     /locus_tag="BM394_RS00820"
                     /old_locus_tag="SAMN04487904_101171"
     CDS             6135..7163
                     /NRPS_PKS="Domain: PKS_ER (39-340). E-value: 1.3e-22.
                     Score: 72.2. Matches aSDomain:
                     nrpspksdomains_BM394_RS00820_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088829.1"
                     /locus_tag="BM394_RS00820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487904_101171"
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_245779889.1"
                     /sec_met_domain="ADH_zinc_N (E-value: 2.6e-18, bitscore:
                     61.7, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSGPTLVPAEPNREVHLGEATGMPLTSEHFRVITTAVPRPGTGE
                     VLVRNRLMAVTAAMCTMMVAEELPIPSYRRGSPLWGAAIGEVVAAGPASDIEPGALVKH
                     GLGWRDYALLKTTAVESIEPTDAHAPGALLSHGAPAWAALRRVARVGNGDTVFVSGAAG
                     GLGTLAGQIARHLGAKRVLGSTGSPEKADRLRRELGYDELVMRNETTVTNQLRLAAPEG
                     LDVVCDTVGGEQLEAAVENANTGARVALLGALSGQLGGDGLSAPTRLDSALLIRRRMEI
                     RGVSLRDHADAEREWTRVFVDGLTEGAFSFPHTRLYGLERAPEALRELVAGEHMGTVLV
                     EL"
     aSDomain        6252..7154
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM394_RS00820_PKS_ER.1"
                     /evalue="1.30E-22"
                     /label="BM394_RS00820_PKS_ER.1"
                     /locus_tag="BM394_RS00820"
                     /protein_end="340"
                     /protein_start="39"
                     /score="72.2"
                     /tool="antismash"
                     /translation="RPGTGEVLVRNRLMAVTAAMCTMMVAEELPIPSYRRGSPLWGAAI
                     GEVVAAGPASDIEPGALVKHGLGWRDYALLKTTAVESIEPTDAHAPGALLSHGAPAWAA
                     LRRVARVGNGDTVFVSGAAGGLGTLAGQIARHLGAKRVLGSTGSPEKADRLRRELGYDE
                     LVMRNETTVTNQLRLAAPEGLDVVCDTVGGEQLEAAVENANTGARVALLGALSGQLGGD
                     GLSAPTRLDSALLIRRRMEIRGVSLRDHADAEREWTRVFVDGLTEGAFSFPHTRLYGLE
                     RAPEALRELVAGEHMGTVLV"
     CDS_motif       6591..6653
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM394_RS00820_0001"
                     /evalue="4.60E-03"
                     /label="PKSI-ER_m5"
                     /locus_tag="BM394_RS00820"
                     /protein_end="173"
                     /protein_start="152"
                     /score="9.8"
                     /tool="antismash"
                     /translation="GDTVFVSGAAGGLGTLAGQIA"
     gene            complement(7201..7863)
                     /locus_tag="BM394_RS00825"
                     /old_locus_tag="SAMN04487904_101172"
     CDS             complement(7201..7863)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602525.1"
                     /locus_tag="BM394_RS00825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487904_101172"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_092972753.1"
                     /transl_table=11
                     /translation="MVKQERAVRTRALVLRAGAEVIATRGYEGATIAEILDHAGVTKGA
                     MYFHFPSKQALAEAVIEEQTNISTVPVTSSRLQDAVDLSQRVARALQSDPLLQAGTRIA
                     VETGFAEEPIVPYQQWTSVFRSMFSSAKESGEVLPSVDPVETAEMFVASYLGVQLFSQA
                     ATNREDVCDRVATLWRHLLPGIATPGVLARIEPDGVGDLDDFGGIPQQHEATDIASS"
     gene            complement(7948..8634)
                     /locus_tag="BM394_RS00830"
                     /old_locus_tag="SAMN04487904_101173"
     CDS             complement(7948..8634)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="BM394_RS00830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487904_101173"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_092972755.1"
                     /transl_table=11
                     /translation="MAMRQERAEQTRCRVVHAAAEVVERDGVRDATLTRIVAVAGVSKG
                     ALYFHFDTKEQLADAVYEAGIARLGKVVAENYRWYHSAFDTLALLCGALVRTLRRDVVA
                     RAMIRLARETDVSSRLSGDPYHHWWLVVYRLLVRARNQREVPSGMDLEAGAGLLVSATV
                     GLEILARNNPAWWNEGSVDRIWHMLLHVREQAVAVTGSSEEVLSEWRLGSEAHSGSALK
                     STARLD"
     misc_feature    8680..8693
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 18.59"
                     /tool="antismash"
     gene            complement(8908..10104)
                     /locus_tag="BM394_RS00835"
                     /old_locus_tag="SAMN04487904_101174"
     CDS             complement(8908..10104)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MGT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1062: glycosyltransferase, MGT family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF044385.1"
                     /locus_tag="BM394_RS00835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487904_101174"
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="WP_175529905.1"
                     /sec_met_domain="MGT (E-value: 5.4e-90, bitscore: 297.8,
                     seeds: 193, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRILFSAIAAHGHCFPLLPLAAAAREAEHEVVVATGVELRAAVHA
                     AGAEFVEAGLPVFDALEAVVSRDDRLEADDPAILGDSAIPGGLPAQQRDRVVGQLFGSV
                     LPRRFVSDIRTLLRSRRFDLVVYETGNPGAALAAELEGVPAVCHGFGRELAGGFTATFG
                     APLLELADELGVSAPADDLGTLGNTYLDVFPPSLQDPAVLALPQRIGIRASPFAESGEL
                     PEAVTARPRDRPLVYLTLGTAFGSADVLCTTARAIAEFDVDLLVSTGPRVRTETLQGLP
                     DNVTVGSWFPQAHLIPWLDLAVHHGGAGTTLTAFAAAVPQLLLPQGADQFDNAASVVTA
                     GAGRSIMPEDNHGQTVVTAVEELLGDSATVRAARYLAAEMAAAPDSAAVVERLSEMYG"
     misc_feature    complement(9940..9942)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(10162..10176)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 18.52"
                     /tool="antismash"
ORIGIN
        1 gcagcaacga caccggtctg atcgcagcgg gaatggccga gttcggcgcc cgccatctcg
       61 acgcacccgc ctaccggcag ggcgtgctca agtgcgtgtt cctcggaatt ccactacggg
      121 acgtctccgg agtgccggaa cgtgccgacg ccgagctggc acgcatgctc accgactacg
      181 ccagggagcg cagcgcggcc ggacgggaga tccccaacga cgtgtggctg gtcgtggacc
      241 cggcacaacc ccactccgcc aaggaggcat gatgcgcatt ttcgacccgc acatccacat
      301 gagctcgcga accacgaacg actacgacgc gatgtaccgg gcaggagtga gggccctggt
      361 cgagccttcc ttctgggtgg ggcagccacg cacgtcggtc ggaaccttcg tggactactt
      421 cgacggtctg atcggatggg aaccgttccg ggcttcgcag tacggtatcg cgcatcactg
      481 caccatcggt ctcaatccga aggaggccaa cgaccccaga tgccgtgacg tactggaggt
      541 actgccccgt tacctggcca aggacggcgt ggtggcggtg ggcgagctcg gttacgacgc
      601 catgaccacc gaggaggacg atgccttcgc cgctcagctg gaactggccg ccgagcacga
      661 gttaccggtg ctcgtacaca ccccgcaccg ggacaaggag gccgggacgc ggcgcagcct
      721 cgatgtgatc cgggagtccg gcatccccgt cgaacgtgtc gtcgtcgatc acctcaacga
      781 ggtgactgtc gaagccgtgg cggaatcggg ctgctggatg gggttttcga tctatccgga
      841 caccaagatg gacgaggacc ggatggttcg gataatgcgc gggtacggcg tggaacggat
      901 gctggtcaat tcggcggccg actggggcaa gagcgatccg ctgaaaacga gcaaaaccgc
      961 gcacgcgatg ctggccgccg gtttcgaacc ggccgcggtt gacaccgttt tctggtcgaa
     1021 cccggtcgga ttctacgggc agagtggtcg actgcgcacg gagacatcca cgctcgggga
     1081 cggtgacggc accactttcg cgggaaactc catcgaacgc gggaaacggt gatcatgcga
     1141 tttcgtcatc gtgacggatc aaccgtacac ctggcctact gcaccaacgt gcatccagcg
     1201 gaagacctcg aaggcatcat cgctcagttc gactactacg ccgtaccgat acgccggcgg
     1261 ctgggcgtcg aacggctcgg gctcggactg tggttggcac gggacgtggc caccacgttg
     1321 gtcgaggacg acaccgcgct ggcccgactg cgcaccgagc tgctggcacg gggcctggaa
     1381 gtggtgacgc tgaacggttt cccctatcgg ggcttccaac gagcggtggt caaacacgcg
     1441 gtgtaccggc ccgactggtc cgagcgcgcg agactggact acacactcga cttggcgaaa
     1501 ctactgtccc atctgctacc cgaagacgtc accagcggca gcgtgtccac actaccgctg
     1561 ggatggggcg cgtggtggtc ggaacaccac cacaaccgag ccctggcaca cctgaccgaa
     1621 ctgaccgacg ggctcgctcg actagccgcg cgggagggac gttcggtgcg agtcgggttg
     1681 gagcccgagc cgggatgcgt gttggagacg accgatcagc tcgtttccgc actgtccgga
     1741 acggacaccg agcacctcgg gatctgtctg gacacctgcc acgtggccgt ggcgttcgaa
     1801 aacccgcacc gagcgctgcg acggctgcac gaagccggcc tgccagtggt caaaacgcag
     1861 gcgtcctccg cgctgcgggt ggacaatccc ggcgactcga cgcaacgtgc ggaactgaac
     1921 tccttcgtgg aacccaggtt tctgcatcag acccagcaga gcggcgacgg ggtccggcgt
     1981 gccgtcgatg atctgggttc ggcgctggca ggcgaactct cgggggatca accgtggcgg
     2041 gtgcacttcc acactccgct gcacactgac ccgagtgcct cgctgcgtgg gacgcatgac
     2101 gtgctcggcg agaccctccg ctcgttgctc ggcgacaaca cggcacacac ccgacacgtg
     2161 gaggtggaga cctacacctg ggatgtactc ccggactcgg gagatcactc cgcgagcatc
     2221 ggtgccggga tcgcggccga actcgactgg gcccggcgcc gactgctcga actcggactc
     2281 acggaggaag gtcgatgaat cacacagccg aggcacgtac tccggtactg ctgctggacg
     2341 tggtgggttt gaccccccga cttctcgaac acatgcccaa tctggaccga atagcgcgcc
     2401 gtggttttcg agccgagctg aaaaccgtgt tccccgccgt cacctgtccg gtgcagtcca
     2461 gtctgctcac cggacgacat cccggtgacc acggcatagt cggtaacggc tggttcttcc
     2521 gtgacatcgg tgaggtcttc ttctggcgcc agtacaacgg gctggtgcgc ggcgaaaaga
     2581 tttggcaagc ggcacgcgag cgggatcccg gctaccgggt cagcaacgtc tgctggtggt
     2641 acgcgatggg gatggacgtc gacgagaccg tgacccctcg tcccgtttac cacgcggacg
     2701 gacgcaagtc gccggactgc tacacctacc caacggggct gcgtgacgag ctcaccgaca
     2761 agttcgggac ctttccgctg ttcaactact ggggaccgac cgcttcgatc gagtccagcc
     2821 gttggatcgc cgaggccacc ggccacttgc tgtccactcg cggttcggac ctgacgatgg
     2881 cctacatccc gcacctcgac tacgacctgc aacgctacgg cccggacagt ccccgagccg
     2941 cggcggcggc acgggaagtg gacagggcgt tggctccgtt gctgaaccgc gcggaagcgc
     3001 aaggagtcac ggtactggcc ctgtcggagt acggcatgac agaggtgagc cggccgatcg
     3061 acatcaatcg cgcactgcgg cgagaacgac tgctgcacgt acacacccag gcgggcatgg
     3121 agtacctcga tccgtggagt tcccgtgcct tcgccgtcgc ggatcatcag gcggcgcaca
     3181 tatacgtccg ggacacagat gatctcgcca gagtacgtga cgtcgtcggt gcgctgcccg
     3241 gtgtcgacga agttctcgac acatcgggca agaaacggta cggcatcgat cacgaacgga
     3301 ccggtgacct ggtggcggta gccgaacccg actcgtggtt cacctactac tactggttgg
     3361 acgacgcacg ggcccccgat ttcgccacgc acgtggagat ccaccgcaag cccggctacg
     3421 acccggccga actgttcatg gatccccgcg accggctggt caggttacgc gcggcgacag
     3481 cggtggcccg caagaaactg ggactgcggt accggatgaa cgtggtaccg ctcgatccga
     3541 ccccggttca cggcagtcac ggcaggctcg cggacagcgc ctcccacgga ccggtgctgt
     3601 tgtgctccga tgaacgcttc ggccgggaga gttaccacgc cacggaagtc aaggacctgt
     3661 tgctcgagct ctccggcctt cgcgaaccgc gacggacaac gcgttgaaca ccaccacggg
     3721 aaaggatgat catgccccga ccgatcacac tgttcaccgg ccagtgggcc gacctgccgt
     3781 tcgaggacgt ggccaaactc gcctcggaat ggggctacga cggcctggag atcgcctgct
     3841 ggggtgatca cttcgaggtg gacagggcgc tcgcggaaaa ggactacgta cggcggaaac
     3901 gagaggtact ggaaaagcac gatctgaact gctgggtgct gtcgaaccac ctgctcggcc
     3961 aggccacctg cgattatccg atcgacgaac gacaccggaa catccttccc ccggagatct
     4021 ggggggacgg tgatcccgaa ggtgttcgac aacgtgccgc ggaacggatg cgggacacgg
     4081 cgagagccgc ggccgagttc ggtgtggaca aagtcgtcgg tttcaccggc tctccgatct
     4141 ggtacacggt ggcgatgttc ccgccgactc ccgacgaaat ggtgcagcgt ggttaccagg
     4201 agttcgccga ccgctggaac cccattctcg acgtgttcga cgagtgtggt gtccggttcg
     4261 cgttggaggt ccatcccacc gagatcgcct acgactactg gaccacggtt cgtactctcg
     4321 aagcggtgga ccaccgatcc gcactcggac tgaacttcga tccctcgcat tacgtgtggc
     4381 agggcttcga tccggtgact tttctgtggg actttcagga ccgcatcctg cacgtcgact
     4441 gcaaggaggc gcgtgtcagg agcaatgacg gccgcagtgg agtgctcggc tcacacctcc
     4501 cctggggaga ccccaggcgt ggctgggatt tcgtgtccac cgggcacggc cacgtcccct
     4561 gggaggactg cttccgcacg ctcaacacga tcggctacga cggtccgatt tccatcgaat
     4621 gggaggacgc gggcatggat cggctgcggg gagctcccga agcgctggag ttcgtgcgga
     4681 acctgaactt cactccgcct gcggcgtctt tcgatgccgc tttcggttcc gagacacagt
     4741 agggcctccc gatctcacgt ggcgaaaact gtcagtcgcc acttccggtg acaccaactc
     4801 caaaaccgac ttggcaaacc gactgcgcgg tttgccaagt ctttccccga caactccacc
     4861 acacggccgc tactgagcac gtccgtgagt tcttcccgcc gcgtcgaagg gcactcccgt
     4921 gtcctcgacg cgcgaggcag gaccgcgaca gggtggacgg cgcacccgac cgaactctgc
     4981 cgcggagcct tgccgaacga tcatcgccgc gtttcgtcgg acaatcgccg gaccagtacg
     5041 gtcgagtcga ccgcgacccg cccttcctgt tccagagtca cccgcacggt gtcccccgcg
     5101 atcggttcgt cgtggacccg cacgaaacac gaccgtccca actcgatgta actgtggaag
     5161 cgggttcgca tacccaccgg cagcaccgtg tccggacaga attcggagcg tgcggcttga
     5221 cgcgccgctt ccagagcggt cattcccgga acatgatcga ccgggtgatc gaacagcacc
     5281 ggatgcgtac gtccgtcccg cagcaaccag gtacgttcgt gaggcgtcgg cgccagcagc
     5341 acatcggacg gacaatcacg accgacccgc cacggcggaa cggactccgg aagttccgcc
     5401 tgatcagccg catcggtgaa gtcgtcacag cctgcccgaa ttcgcgcgta agccgcgggg
     5461 gatatgcaac tgtacggcag cgtgaccctc ccgacgaaga ccccgtcacg atatccgtgc
     5521 acgtgcatat tcgccccggc gaatgtccta ccccgccatt tgacgttttc gcagtgaatt
     5581 tccagtacga tttgggcggg ggtatcggcg agacgaagcg cttccggctt cacctcgtaa
     5641 tccaatgtgt ccattatgaa ctggtgcccg tagggaacgc cgaagccgac gtgccccacg
     5701 accaaccctg cctgccgcgc cgcctccgcg aacaggagag gatcgtgacc acccgcgacc
     5761 ggcccgtaaa gggcgtgcgc acgaggtaat tgtgccccca gcacgaaatc gttctcgccg
     5821 cgttcaccga aactggtaac cagaacctcg gtattcgcgg cacgatggac ccagcgacgt
     5881 ggcagactct gctcgaacga gacggttggt tgctcacggg actcttcggt gacggtgtcg
     5941 gcgacgatac tgctggtcat aaggtcctcc acagcattaa agatacctac cgtgcggttt
     6001 gaaatccccc tccatgagga tagagaccgg ttagtcggtt tttcaagagt gtagtgagta
     6061 gtcttgaagg gaaacttcgg tcagccggct cgccgttcgg gctcgccggt tcgacgatga
     6121 ttggtggtgt tgcggtgact tccggaccaa cgctcgtgcc cgcggaaccg aaccgggaag
     6181 tacatctcgg cgaagcgacg ggaatgccac tcacctcgga acacttccgc gtgatcacca
     6241 cggcggtgcc tcgccccggc accggcgaag tcctggtgcg caacaggctc atggcggtga
     6301 cggcggcgat gtgcaccatg atggtcgccg aggaactccc gataccttcc tatcggcggg
     6361 ggagtccact gtggggtgcc gccatcggcg aggtcgtggc cgcggggccg gcgagcgata
     6421 tcgaacccgg tgccctggta aagcacggac tgggttggcg cgactacgca ctgctgaaaa
     6481 cgacggcggt cgagtcgata gagcccaccg acgcacacgc ccctggcgca ctgctctcgc
     6541 acggtgctcc cgcctgggcc gcgttgcgca gggtcgcgcg ggtcggaaac ggggacacgg
     6601 tcttcgtgag cggggcggca ggtggcctcg gcaccctggc cggacagatc gcgcgacacc
     6661 tcggcgcgaa acgagtgctg ggaagcaccg gctcaccaga gaaggccgac cggctgcgtc
     6721 gagaactcgg ttacgacgaa ctcgtgatgc ggaacgagac cactgtcacg aatcaactgc
     6781 gactggccgc accggagggt ctggacgtcg tctgcgacac cgtgggcggc gaacagctcg
     6841 aagccgcggt ggaaaacgcc aacaccgggg cacgtgtggc gctgctgggg gcgctgtcgg
     6901 gccaactcgg tggggacgga ctcagcgcgc cgacccgctt ggactccgcg ctgctgatca
     6961 ggcggaggat ggaaatccgg ggtgtctcgc tacgggatca cgcggacgcg gagcgggagt
     7021 ggacccgcgt gttcgtggac ggtctcaccg aaggggcttt cagctttcca cacaccaggc
     7081 tgtacggact cgaacgagcc cccgaagcac ttcgtgagct cgtagccggc gaacacatgg
     7141 ggaccgtact ggtggagctc tgagaactcc gccgcgggag ggcgttcagg cacagcggga
     7201 tcacgaggac gcgatgtcgg tggcctcgtg ctgctgggga ataccaccga agtcgtcgag
     7261 atcaccgaca ccgtccggtt cgattcgtgc cagcaccccc ggagtcgcga ttccgggcag
     7321 cagatgacgc cacaacgtgg caacccggtc acatacgtcc tcgcggttgg tagcggcctg
     7381 cgagaacagc tgaacgccca agtaggaggc gacgaacatc tccgcggtct ccaccgggtc
     7441 gacggagggc aacacttccc cgctctcctt ggcggaggag aacatcgaac ggaacacact
     7501 ggtccactgc tggtacggaa cgatcggttc ctcggcgaac cccgtctcca cggcgattcg
     7561 cgtacccgcc tgcaacaacg gatcggactg caacgcccgg gccacgcgct gcgagagatc
     7621 gaccgcgtcc tgcaaccgcg agctcgtcac cggcacggtc gatatgttgg tctgctcctc
     7681 gatcacggcc tccgccaacg cctgcttgga cgggaagtgg aaatacatcg cccccttggt
     7741 caccccggcg tgatcgagga tctccgcgat cgtcgcccct tcgtaaccac gtgtggcgat
     7801 cacctcggcc ccggcccgca gcaccagggc cctcgtccgc accgcgcgtt cctgcttaac
     7861 caagctctcc tccagtacgt tgccggacca ccgatatccc tacgtcaacg accgggacac
     7921 gtcggattca ctgtttcggt caacatatca atccaaccgg gcggtacttt tcaatgctga
     7981 accactgtgc gcctcggaac cgagccgcca ctcggacagc acctcctccg aggagcccgt
     8041 aaccgcgacc gcttgctcgc gcacatgcaa cagcatgtgc cagatgcggt ccacactccc
     8101 ctcgttccac catgcgggat tgtttcgcgc caggatctcc aggcccaccg tggccgagac
     8161 caacagaccg gcccccgcct cgaggtccat accgctgggc acctcgcgct ggttacgagc
     8221 tcgcaccaac aagcgataca cgaccagcca ccagtggtgg tacggatcac ccgacaaccg
     8281 gctgctgaca tccgtctccc gagccagtct gatcatcgca cgggcgacca cgtcacggcg
     8341 gagagtacgc acgagggccc cgcacaacag agccagcgtg tcgaacgccg agtggtacca
     8401 gcgatagttc tcggccacca cttttccgag tctcgcgatc ccggcctcgt aaacggcgtc
     8461 cgcgagctgc tccttggtat cgaaatggaa gtacagtgcc cccttcgaga cgccggcaac
     8521 cgccacgatg cgagtcagtg tcgcgtcccg cacaccgtcg cgttcgacca cctcggcagc
     8581 ggcgtgcacc acccgacagc gggtctgctc ggcccgctcc tgcctcatcg ccatcacgac
     8641 atgctccctg tctcgtctct aataaaacca actaagcggt ttaaatattt tcactgtact
     8701 gccgacggac aggaagaacc aacggccgaa caggcacagg taaggtgaac actctccgtg
     8761 tcctcgcgtg atgcggggca gaacgcgccc acgaggtggg cgacgaggcc ggccgacaac
     8821 gaacgagaac gtgcgatgaa tttcatcaca cgggttcgtc ccgccttcgg gtccgtactg
     8881 aaccgaaccg cgaggtcccc gctcgcctca gccgtacatc tcggaaagtc gttccaccac
     8941 agcggcggaa tccggcgccg cggccatttc ggccgccagg taacgcgccg cccgcaccgt
     9001 ggccgaatcg ccgagcaact cctccaccgc cgtgacaacc gtctgcccat ggttgtcctc
     9061 gggcattatg ctgcggcccg ccccagcggt tactacgctc gcggcgttgt cgaactgatc
     9121 agcaccctgc ggcagcagca actgcggaac cgcggcggcg aacgcggtca gcgtggtgcc
     9181 cgcgccaccg tgatgcaccg ccagatccag ccaaggaatc agatgggcct gcgggaacca
     9241 ggacccgacc gtgacgttgt ccggcagacc ttggagcgtc tcggtacgca ccctgggacc
     9301 tgtcgagacg agcaggtcca cgtcgaactc ggcgatcgct cgcgcggtcg tacacaacac
     9361 gtcggcgctg ccgaaagcgg ttcccagtgt cagatagacg agcggacgat cacgcggtcg
     9421 tgcggttacc gcttcgggta gttcaccgga ttcggcgaag gggctcgctc gtataccgat
     9481 ccgctgggga agcgcgagca ccgccggatc ctgcagggac ggtgggaaga cgtccagata
     9541 agtgttcccg agggtcccga ggtcgtcggc gggagcggaa acccccagtt catcggccag
     9601 ctccagcaac ggagccccga acgtggccgt gaagcccccc gcgagttccc taccgaatcc
     9661 gtggcaaaca gccggtaccc cctcgagctc cgcggccagc gcggcccccg ggttaccggt
     9721 ttcgtaaacg acgaggtcga atcgacggga gcgcagcaga gtacggatgt ccgagacgaa
     9781 acgacgcggc agcacactgc cgaacagctg ccccacgacc cggtcacgtt gctgtgccgg
     9841 gagaccaccg gggatggctg agtcgccgag gatggctggg tcatcggcct cgagacggtc
     9901 gtcacggctc acgaccgcct cgagagcgtc gaagacgggt aaaccggcct cgacgaactc
     9961 ggcacccgcc gcgtgaacgg ctgctcgtag ctcgacgccg gtggccacga ccacttcgtg
    10021 ttctgcctcg cgtgccgccg cggcgagagg caacagcgga aaacaatgcc catgcgccgc
    10081 tatggcggaa aacaggatgc gcacgctgcc aaaactagta gccgaagaac gatgttgcca
    10141 tcttttcaca cgagaatgga cttttcgagt ggagaaccaa cactcgaatg cgggcccgcg
    10201 aggcatggca gaaccaccag aaacgtgttg ccccgcggat tccgcgtcag cacgaccgtg
    10261 cggcccaccc gagtcgatcg aagcgccgga tcgggttgtc cgcacggcgg gcaacgacgt
    10321 agaatctcgc cggttctgct gccgtcgcgg ggagggaaag tgcgccgctg aggtctcgag
    10381 acatcgcgtg cccctctggt acgcagtgac gtgacctcgg atcgtgtgcc atcccaagcc
    10441 cagttgcggt cttctttccg gccgtcccgt tgtgtggtgg cgcggtgtct ccaaacgccg
    10501 gctgatcgag tcgaaaactc tctggaggca gaaccatgtc cacacgcgac cggactctcg
    10561 tcgaatgcgc cgccgtttcc ttcgagtggc cggacggcac agtagtactg cacgatctcg
    10621 actggtccgt ggcagcggga cgaaccggag tcgtcggttc gaacggaacc ggcaagtcca
    10681 ccctgctgga actgatcacc ggcgggttgt cccctcgatc tgggaccgta cgaacgtccg
    10741 gtgagctcgg ctatctgccg caacggctca ctttcgacga aacggcgcgt gtagcggacg
    10801 ctctcggcgt caccgcccgc ctggcggcac tgcgagccat cgaggccggg gaagcccggg
    10861 aggagcattt caccgcgctc ggcgaggagt gggacatcga ggaacgctgt ctggggatgc
    10921 tggacggatt cggtctcggg catctcctac tcgacagcgt
//
