LOCUS       NZ_FPAA01000014        10384 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Marininema halotolerans strain DSM 45789, whole genome shotgun
            sequence.
ACCESSION   NZ_FPAA01000014
VERSION     NZ_FPAA01000014.1
KEYWORDS    WGS; RefSeq.
SOURCE      Marininema halotolerans
  ORGANISM  Marininema halotolerans
            Bacteria; Bacillota; Bacilli; Bacillales; Thermoactinomycetaceae;
            Marininema.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900116235.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 02:20:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,453
            CDSs (total)                      :: 3,387
            Genes (coding)                    :: 3,359
            CDSs (with protein)               :: 3,359
            Genes (RNA)                       :: 66
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:12:26
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 36808
            Orig. end                         :: 47192
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FPAA01000014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10384
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10384
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10384
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5384](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5384)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(567..1583)
                     /locus_tag="CHX35_RS15300"
                     /old_locus_tag="SAMN05444972_11441"
     CDS             complement(567..1583)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1251: luciferase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390038.1"
                     /locus_tag="CHX35_RS15300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444972_11441"
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="WP_091839056.1"
                     /transl_table=11
                     /translation="MIKLSVLDQSPIPKGKTAIEALADTSRLAQETEKMGYHRFWVSEH
                     HFSKSLAGSSPEVLISHLAAKTNHMRFGSGGVMLPHYSPYKVAENFKVLEALYPKRIDV
                     GLGRAPGGLPLATRALQEGKKVTRDPYPQQLEDLITYLYDQTDENHRFPQLLATPLVDS
                     HPEVWLLGSSGGSAHLAAQHGASYAFAQFINGEGGEEVVQFYRDHFRPSLINSHPHILL
                     AIFVICADTEEEAERQASVLDLSLLLLQKSAGNPAGIPSIEDAQSYPYTEMDRAIIRHN
                     RQRMIVGNSEQVKERILALGDSYGTEEFMIVSILHDMEAKLRSYQLVADAFRDELSS"
     gene            1768..3594
                     /locus_tag="CHX35_RS15305"
                     /old_locus_tag="SAMN05444972_11442"
     CDS             1768..3594
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /locus_tag="CHX35_RS15305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444972_11442"
                     /product="penicillin-binding protein 2"
                     /protein_id="WP_091839058.1"
                     /transl_table=11
                     /translation="MVRKHWRSKWIAVLLFFAFVSILVRLYWIQISAVHSFSREKADLV
                     ARAQEQQNRDFIVDSGRGAILDRRGRPITYEKDWRLVVFPLSERQVSTNQKQLTRVAQI
                     LHISVQSLSKQLTAIHRPEALKKDGNSDWILTQGQAEAISKLAIPGIYARRSDDRSGFS
                     LAKTVIGRLERNPFLIQERYPDEWEKGMYDSRSRIGVTGLEETFEPFLRGDGGEYLLFE
                     KDGRGRPLNGTTVIKEKGNEAFYPYRVVTTLDMDIQRLTERILQKQKVEEGAIVVQDIA
                     SGDILAMASQPDESTAKDEQNPWDNRALMETTPGSIFKTVVALAALDTGKVKMTDVFHC
                     NGSLGRYGLTDTHAHGKETFAQAYANSCNIVFAQIAEKVGAKTIELYAQRMGLGQRMIW
                     TGELFQEKNFKQLQGEEKGLIFAPGTSKEDKGAIAQTGIGQRDVRVTPLQAANMVTTLF
                     HNGSTPNPRIVKEIRRYDGSPVVTFPDHVLKTKERIGSRALAQVREMMRGVVTKGTAQS
                     MKTAILPLAGKTGTAQVGVKGDHYNKWMIGYAPYDKPRWAVSVVVRSSADPKEMRGQQI
                     FRQVMEEIYRLDQQQKKEKQEKKKPPSKSNND"
     misc_feature    3659..3673
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.85"
                     /tool="antismash"
     misc_feature    complement(3661..3672)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.34"
                     /tool="antismash"
     regulatory      3671..3808
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            3968..4540
                     /locus_tag="CHX35_RS15310"
                     /old_locus_tag="SAMN05444972_11444"
     CDS             3968..4540
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709654.1"
                     /locus_tag="CHX35_RS15310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444972_11444"
                     /product="ECF transporter S component"
                     /protein_id="WP_091839060.1"
                     /transl_table=11
                     /translation="MTKQGSTMKLTTVSILAGFAFLMQYLNFPLPMFPPFLKVDFSELP
                     ALVAGLMFGPLAGVLVEFLKNLLHYLFSGSDTGIPIGQLANFVAGSIFVVTTVLITRKL
                     QGIKGLVTGLTVATILMAVIMSIANYWVILPAYAFLIHWEVVGPAKTSLVLYGIGPFNL
                     VKGVLTALLFIPLYQRLKGRIHLPKIS"
     gene            complement(5001..5384)
                     /locus_tag="CHX35_RS15315"
                     /old_locus_tag="SAMN05444972_11445"
     CDS             complement(5001..5384)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709647.1"
                     /locus_tag="CHX35_RS15315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444972_11445"
                     /product="ectoine synthase"
                     /protein_id="WP_091839061.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.5e-53, bitscore:
                     172.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVKHLADLIGTENEVKGDTWESRRFLLKKDNVGFSMHDTIIKAG
                     TTTTMWYKNHIEAVYCVQGEGEVETLDDGKVYPIKDGTIYTLNGHERHQVRAKSDLRLV
                     CVFNPPCTGLETHDEDGAYPLLD"
     gene            complement(5412..6695)
                     /gene="ectB"
                     /locus_tag="CHX35_RS15320"
                     /old_locus_tag="SAMN05444972_11446"
     CDS             complement(5412..6695)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (33-366). E-value: 1.2e-80.
                     Score: 263.0. Matches aSDomain:
                     nrpspksdomains_CHX35_RS15320_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064921.1"
                     /locus_tag="CHX35_RS15320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444972_11446"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_091839063.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.6e-85, bitscore:
                     278.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLTSITEATTDMSTFETLESEVRSYCRSFPTIFDKARGYKLWDVE
                     GREFIDFFAGAGALNYGHNNPQIKSKLIDYLTNDHVVHSLDMATVAKEKFLQRFQQVIL
                     EPRKMNYKVMFPGPTGANSVESALKLARKVTGRQTVISFTKAFHGMTLGALSVTGNSFK
                     RHGAGVPLHHAVSMPYDNYHDDVDSLSLLERYLEDEGSGLDLPAAIILETLQGEGGINV
                     ASNTWLQRIEDICRRWDILLIVDDVQVGCGRTGTFFSFEEAGIQPDMICLSKSIGGYGL
                     PFAITLIQPKLDKWAPGEHNGTFRGHNLAFVAATEALTYWETDKLEREILRKGEILQQA
                     LNSMTAKHPDTEPEVRGRGLMQGIAFGQEGFAEKVCAAAFERGLIMETSGPDSEVAKVM
                     PPLIIDDEGLNKGLAILEESIQAAKNTR"
     aSDomain        complement(5598..6596)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CHX35_RS15320_Aminotran_3.1"
                     /evalue="1.20E-80"
                     /label="CHX35_RS15320_Aminotran_3.1"
                     /locus_tag="CHX35_RS15320"
                     /protein_end="366"
                     /protein_start="33"
                     /score="263.0"
                     /tool="antismash"
                     /translation="DKARGYKLWDVEGREFIDFFAGAGALNYGHNNPQIKSKLIDYLTN
                     DHVVHSLDMATVAKEKFLQRFQQVILEPRKMNYKVMFPGPTGANSVESALKLARKVTGR
                     QTVISFTKAFHGMTLGALSVTGNSFKRHGAGVPLHHAVSMPYDNYHDDVDSLSLLERYL
                     EDEGSGLDLPAAIILETLQGEGGINVASNTWLQRIEDICRRWDILLIVDDVQVGCGRTG
                     TFFSFEEAGIQPDMICLSKSIGGYGLPFAITLIQPKLDKWAPGEHNGTFRGHNLAFVAA
                     TEALTYWETDKLEREILRKGEILQQALNSMTAKHPDTEPEVRGRGLMQGIAF"
     gene            complement(6699..7304)
                     /gene="ectA"
                     /locus_tag="CHX35_RS15325"
                     /old_locus_tag="SAMN05444972_11447"
     CDS             complement(6699..7304)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: protein motif:HMM:TIGR02406.1"
                     /locus_tag="CHX35_RS15325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444972_11447"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_342743140.1"
                     /transl_table=11
                     /translation="MKDGDLAINDRQSPSPLKTDDSLRSKSSVKKYRHPGNQVTFRKPT
                     EEDGTAVWKLIKGTGVLDLNSSYSYLMLCKYFSETCVLAEQEGRVVGFVSAFVQPESPD
                     VVFVWQVAVDQSQRGKGLAKSLLRNLLNREACENVRFLEATVSPSNNASQSLFKGLARD
                     IQTTCHTSECFSEDLFPGDDHESEWTYRVGPFENIEEV"
     misc_feature    complement(7410..7423)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 20.35"
                     /tool="antismash"
     gene            7852..8655
                     /gene="ehuB"
                     /locus_tag="CHX35_RS15330"
                     /old_locus_tag="SAMN05444972_11448"
     CDS             7852..8655
                     /GO_component="GO:0009897 - external side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006212832.1"
                     /locus_tag="CHX35_RS15330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444972_11448"
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_245838879.1"
                     /transl_table=11
                     /translation="MQFFSSGESALQEAKDQKSVVIGFANEKPYAYQEDGKVTGEAVEI
                     AKKIFKNQGITNIKPVLTDFGSLIPGLKAGRFDVITAGMYITPERCKQVAFANPEYNIG
                     EGLAVQKGNPKDIHSYKDVANQPDTTIAVMKGAIEESYMKDSGVKENQIKFVADQPTAV
                     ALLNSGRVDGVTMTGPSLLAILQSAKAKNVSVVEDFKQPIIDGKEIKGIGAAAFRPEDK
                     DFVKIYNDGLAQMKESGELYDTIHPFGFTKKDLPGDETAEEICKQ"
     gene            8717..9346
                     /gene="ehuC"
                     /locus_tag="CHX35_RS15335"
                     /old_locus_tag="SAMN05444972_11449"
     CDS             8717..9346
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709636.1"
                     /locus_tag="CHX35_RS15335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444972_11449"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_342743139.1"
                     /transl_table=11
                     /translation="MKNLLPGAVPTIQLTILGAMLAFVLSFMGGLGRLSRFAVVRGLAG
                     FIVEFFRGTSLLVQLFWLFYVLPFMGIRLESGMVGLLALGLNYGAYGSEVVRSAVLSVP
                     KGQHEAGIALNMTAFQRMRYIIIPQAFRTMIPSFGNLLIELLKGTALVSLVTIPEMTFE
                     ALVMRTSDAAHTLEIFTWLLLIYFAIAYPLTMLMRWVERRYSLGRL"
     gene            9353..10009
                     /gene="ehuD"
                     /locus_tag="CHX35_RS15340"
                     /old_locus_tag="SAMN05444972_11450"
     CDS             9353..10009
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709635.1"
                     /locus_tag="CHX35_RS15340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444972_11450"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_091839118.1"
                     /transl_table=11
                     /translation="MWDWQYAFEILPRLLKVAHVTITATLGAFVVAMILGLVLAFARRS
                     TNRLLSGVTYFVVEFIRSTPPFVQLFFLFFVMPQFGITMGALMTGILGLGIHYSTFLSE
                     VYRAGIDSVDKGQWEAATALNFTSRQKWVHIILPQAIRPIIPVMGNYLITLFKETPMLL
                     GITVVEMLSQAQIIGSQSFRYTEPITMVGIFFLILSYGASLLVKRIELRMDINRR"
ORIGIN
        1 tgcaagctta tcgtattgct aaaccggaag cagggaattg gaaagtagaa atgacgagta
       61 agaagaatga tgcttacttt atgttagcgg cctttaatgg agcagatcaa gccttcgatg
      121 tggatgtgga taccaaaggt agccaacaag atgaaatccc tgtagatatt aatgtattgg
      181 atacggagaa atatgatccg caaaccgtca aggtagcaat gaaggttgtt gattccaatg
      241 gcattgaaca atatgtcaat caacaaagaa taaagaagct aaagatgatc aaaaagaaaa
      301 agcatcgcgg tcaatttcaa ggtgaattcc gtacacgtaa acaggatggc gtctataaca
      361 tcaccattga aattaacggt aaaatgaaga atggagaacc gtttaaacgg acgctggttc
      421 gttctgtaca tgtagggaac taatcagatg atgtaaaacc accctctctt tcaggtgaaa
      481 gagagggtgg ttttttgtcg tgtgaaaagg ataaacgagt gggtgcaagg cgttttcgcc
      541 gctcactggt tatcgaagga ttgattttat gatgagagct cgtctcgaaa agcatcggca
      601 acgagttgat aggagcgtaa cttcgcctcc atatcatgaa gaatactcac aatcataaat
      661 tcctcagtgc cataggagtc acctaaagcg agaatgcgtt ctttaacctg ctcgctattt
      721 ccaacgatca ttcgctggcg gttgtgacga atgatggctc gatccatttc ggtataagga
      781 taactctggg catcttcgat gctaggaata cccgctgggt tgccggcgct tttttgcaga
      841 agaaggaggg ataaatccaa gaccgatgct tgtcgttctg cttcttcctc agtatctgcg
      901 caaataacga agatggcaag gaggatatgg gggtgtgaat tgattaatga agggcggaaa
      961 tgatcgcgat agaactgtac gacttcctct cctccctctc cattaataaa ttgagcaaag
     1021 gcgtagctcg ccccgtgttg tgccgctaaa tgtgcactgc ctccgctaga accaagcaac
     1081 catacttcag ggtgactatc aacaagggga gtggcaagaa gttgaggaaa tcgatggttt
     1141 tcatctgttt gatcatataa ataggtaatc aaatcctcta attgttgtgg atagggatcc
     1201 cttgttacct tcttcccttc ttgaagggcg cgtgtagcga ggggaagtcc tccaggagct
     1261 cttcctaaac cgacatcaat cctttttgga tagagcgctt caagtacttt gaaattctcc
     1321 gctactttat atggactata gtgggggagc attacgccac ccgaaccaaa gcgcatatgg
     1381 ttggtttttg cggcaaggtg actaatgagt acctcggggc tagaccctgc tagacttttt
     1441 gagaaatgat gttcggatac ccagaagcga tggtatccca ttttttctgt ttcttgtgct
     1501 aatcgactgg tatctgctaa ggcttcaata gccgtttttc ccttgggaat tggcgattga
     1561 tccagtacgc tcaatttaat cataaactca cctcaagctg cttggtttga tcatgttgta
     1621 tatcagagga tcgacactaa tattcccatc cctaggcata gcgaaacatg acacacatca
     1681 agggggattt taacttttaa aatagaggat gtgtttaggc tcctcatctc ctgacgagac
     1741 tgctaaggaa gaggagtgac gaagggtatg gtgagaaaac actggcgcag taagtggata
     1801 gcggtattgt tgttttttgc ttttgtcagc atcctggttc gattatattg gattcaaatc
     1861 tccgcagtgc actcgttttc tcgggagaag gctgatttgg ttgctcgtgc gcaagaacaa
     1921 caaaatcgag actttattgt ggatagtgga aggggagcca tccttgatcg acgcggtcga
     1981 cccattacct atgaaaagga ttggcgctta gttgtctttc cactctcgga acgtcaggtg
     2041 tctaccaatc aaaaacaatt aacgcgtgtt gcacagattt tacatatatc tgtccagtca
     2101 ttaagtaagc aattgacggc aattcatcgt ccagaagcct taaaaaaaga tgggaacagt
     2161 gattggattt taacgcaagg ccaagccgag gcgattagta aacttgcgat tcctgggatc
     2221 tatgcacgtc gttcggatga tcgcagtggg ttctccttag caaaaacagt gattggaaga
     2281 ttggagagaa atcctttttt gattcaagag cggtatcctg atgaatggga aaaggggatg
     2341 tatgattcac ggtcgcggat tggcgttact ggattagagg agacctttga acccttcctt
     2401 cgtggagacg gaggagaata tctactcttt gagaaggatg gacgggggcg gcctctaaat
     2461 ggcaccacag tgatcaagga gaagggtaat gaagcgtttt atccttatcg tgtagtgaca
     2521 acgttggata tggatattca gcgcctaacg gagaggattt tacaaaagca gaaagtagaa
     2581 gaaggggcca ttgtggtaca agatatagcc agtggagata tcctcgcaat ggcgagtcaa
     2641 cccgatgaaa gtacagcaaa agatgagcag aatccatggg ataaccgtgc actgatggaa
     2701 acaacccctg gctccatttt taagacggtt gttgcacttg ccgcactcga tacaggaaaa
     2761 gtaaaaatga cggatgtttt tcactgcaat ggaagtttgg gtcgttacgg attgacagat
     2821 acccatgctc atggaaagga aacatttgcg caggcatacg cgaattcctg taatattgtt
     2881 tttgcccaga ttgcagaaaa agtaggagcc aaaaccattg aattgtatgc ccaacgcatg
     2941 gggttaggtc aacgaatgat atggacaggg gagctatttc aagaaaaaaa ctttaagcaa
     3001 cttcagggag aggagaaggg gttgattttt gcacctggga catcgaaaga ggacaaggga
     3061 gcgattgcgc agacgggaat tgggcaacgt gatgtaagag tgacgccttt acaagcagcc
     3121 aacatggtga ctacgttatt tcacaatggc agtaccccta atccacgcat cgtaaaggag
     3181 attcggcggt atgatggttc gcctgtcgtt acttttccgg atcatgtgtt aaaaacgaag
     3241 gagagaatcg gctcgcgtgc acttgcacag gtgcgggaga tgatgagggg agtcgtgacg
     3301 aagggcactg cacagtcgat gaaaacagcg attcttccct tggcagggaa gacaggaacg
     3361 gctcaggttg gtgtgaaggg agaccactat aataagtgga tgatcggtta tgctccctat
     3421 gataagccgc gatgggcggt atccgttgtc gttcgctcat cggcggaccc caaagagatg
     3481 cgagggcaac agatttttcg ccaagtaatg gaagaaatct accggctaga ccagcaacaa
     3541 aagaaggaga agcaggagaa aaagaaacca ccaagcaaat cgaataatga ttgaatgctc
     3601 attcataatc gtgtcccccc ttgccaaatc gatcgttgcc tcgtaaaatg gaggaaagtg
     3661 aataacgttt tcaatccttc ggggcagggt gaaattcccg accggcggtg atggtaccta
     3721 tgagtacctc agtccgtgac ccgctttatg cggtggacct ggtgaaaatc cggggccgac
     3781 agtatagtct ggatgggaga aggatgacac gtacgcgtgt ggatgcgtag gtctatgaaa
     3841 attcgagacc tttatttgag agtgtccaat agaaattaaa cccgaaggaa ggctaatgca
     3901 tggcctttct tcgggttttt cgctgtacgg cgtcatcaga acacataaga aaagggagtg
     3961 ttctctgatg acgaaacagg gttcaacaat gaaattgaca accgtttcga ttttagctgg
     4021 atttgcattt ttaatgcagt atttaaattt tccactgccg atgtttccgc catttttaaa
     4081 ggtggacttt agtgaattac ctgctttggt agctgggttg atgtttggac ccttggcagg
     4141 tgtacttgtg gaatttctta aaaatcttct tcactattta tttagtggga gtgatacagg
     4201 gatcccgatt ggtcaattgg ctaattttgt tgcaggtagt atctttgtgg tgacgacggt
     4261 tcttatcaca cggaagctac aagggattaa agggctggtc acgggtttga cagtggctac
     4321 gatactcatg gctgttatca tgagtatcgc gaattactgg gtgatcttac ctgcttacgc
     4381 tttcctcatt cattgggaag tggtcggacc agcaaagacg tcgttagttc tttatggaat
     4441 cggtcccttt aacttagtaa agggagtatt gactgccctc ctctttatcc ctttgtatca
     4501 acgactcaaa ggaagaatac atcttccaaa gatatcttaa caatctacag ggatattaat
     4561 gaatgattct gaaaacgtta ttcatgaccg gccgatcgac ggtcggtttt ttatttgggt
     4621 ggttaacgaa aggtaacaat gattccagcg atcaacatga tataaccaat tacaggaaga
     4681 accaggggta tgcggtgcac cggatatcct cctttcttaa gtaatcgccc tttgaaggcg
     4741 ggatgtggtt gagggttcat tatgaatggg agttcccgtt atggcaagat ttaaacgtcc
     4801 tctcattata atgacgaacg aggacggata aaagttgcaa agaccgcacc tttttttaag
     4861 gtgcggtctt tgcaacttag cctgcaattg tcggtgtcat cctacttcga ttggcaataa
     4921 aacccggtcg tggtggttga ccgaatggtc tacatgtcgt tgaatgtgaa gtggaagaaa
     4981 gacgatagaa ttcctattcc ttaatcgagt aaggggtaag caccgtcttc atcatgggtt
     5041 tcaagtcctg tgcaaggagg gttgaagacg caaacgagac gtaaatcgga ttttgcccgc
     5101 acttggtgac gttcatgtcc atttaacgta taaattgttc cgtctttgat cggataaact
     5161 tttccatcgt caagggtttc tacttctcct tctccctgca cacaataaac ggcttcaata
     5221 tggtttttat accacatggt ggtggttgtt ccagctttaa taatcgtatc gtgcatggag
     5281 aagcccacat tatctttttt caagagaaaa cgtctacttt cccaagtgtc tcccttgact
     5341 tcgttttcgg taccaatgag atctgcgaga tgtttgacga tcacgaggtg ttcatccttt
     5401 cactataggg tttagcgtgt gttttttgct gcttggatcg attcctccaa gattgcgagg
     5461 cctttattta atccttcatc gtcaatgata agtggaggca tcaccttggc aacttcacta
     5521 tcaggccctg aagtttccat gattaaacca cgctcaaagg cggcagcaca tactttttct
     5581 gcaaatcctt cttggccaaa agcgattcct tgcatcagtc cgcgaccgcg tacctcgggt
     5641 tccgtgtcag ggtgtttcgc tgtcatggaa ttcaaagcct gctgaaggat ttcgcctttg
     5701 cgaaggattt cccgttccag tttatccgtt tcccaatagg ttaacgcttc tgtagcggca
     5761 acaaaggcca agttatgacc gcggaatgtg ccattatgct cacctggcgc ccatttatcc
     5821 agcttaggtt gaattaacgt gatggcaaat gggagaccat atcccccgat ggatttggat
     5881 aggcagatca tatcaggttg aatacctgct tcttcaaaac taaagaaggt gcctgtacgc
     5941 ccacaaccaa cctgtacatc atcaacaatg agcaggatgt cccaacggcg gcaaatatct
     6001 tcgatccttt gtagccaagt gttgctggca acgttgattc cgccttcgcc ttgtagcgtt
     6061 tctaggatga tggcagcggg taaatccagg ccacttcctt catcttctag atagcgttca
     6121 agtaggctca gggaatctac gtcatcatga taattgtcgt agggcattga aacagcatgg
     6181 tggagaggaa cacccgcacc atgtcgttta aaggaattac ctgtgaccga gagcgcacca
     6241 agcgtcatcc catggaaggc tttcgtaaag ctaatgacgg tttgtcgtcc agttactttt
     6301 cgtgcgagct ttaaagcact ttcaacggaa tttgcaccgg tcggtcctgg aaacatcact
     6361 ttgtaattca tcttccgcgg ttcgagaatc acttgttgaa aacgttgtaa aaacttttcc
     6421 ttagcaacgg ttgccatatc tagactgtgc acaacatgat cgttggtaag gtagtcaatc
     6481 agtttgcttt ttatttgagg gttgttgtga ccataattta atgctcctgc accagcgaag
     6541 aaatcaataa attcacggcc ttcaacatcc cagagcttat acccacgtgc cttatcaaaa
     6601 atggtaggga agctacggca gtagctacgt acctcggatt ccaaggtttc aaatgtgctc
     6661 atatctgtag ttgcttcggt tattgaagtc aaaatgaatt aaacctcctc aatgttttca
     6721 aacggcccta ctcggtatgt ccactcagac tcatggtcat ctccaggaaa taaatcttcg
     6781 gaaaaacatt cagaggtatg gcaggtcgtt tggatatctc ttgccaagcc tttaaatagg
     6841 gactgagaag cgttgtttga aggtgagacg gtagcctcga ggaagcgtac attttcacat
     6901 gcttcccggt tgaggaggtt ccttagcaag gatttggcca acccttttcc acgctgactt
     6961 tgatcaacgg cgacttgcca tacaaagacc acatcagggg attcaggttg cacaaaggca
     7021 gaaacgaaac caacgacacg cccttcttgt tccgccaata cacatgtttc agagaaatat
     7081 ttacacagca tgagatagct gtaacttgag tttaaatcta aaacacccgt acctttaatc
     7141 agtttccata cagcagtacc gtcctcttca gtaggtttac gaaacgttac ttgattacct
     7201 ggatgacggt acttttttac ggaacttttt gatctcagcg aatcatctgt cttcagtgga
     7261 gacggactct gtcgatcatt gatagctaga tcaccatcct ttattgatta atatcatcag
     7321 taaaactacc cgtcttttat cgtaacgtgt acggaaccat ccgtcgaaag tactgtaccc
     7381 gcttagatta cggttaaaca tggcaggact tgatgagggt caatgagggc tgagattcta
     7441 acatatcctg tggaatgata cgaatcagac tccatgttat ctaaaaaggg atatacagaa
     7501 gattgtctag tataatcact gatttttctt catattaatt gatccatagg ggattagcag
     7561 tttttaattt cattcgatgg acatgttatc caatttgcta atgattattt ttggtgaaaa
     7621 aataggtttc atccggtatg ttttgtgata tgtaaagaaa ggataggcat tttttaatga
     7681 ttttaaacgg attctccaat tatgcggcaa tagggaggtt gtaaggaggg atttgcatat
     7741 gaaatccgct ataattggac attttcaaaa ggagggattg gcattttaaa gatcaggaaa
     7801 gaagtgttca tattggttct tctcatcttc ctggttacgg gatgtgggcg attgcagttt
     7861 ttttcttcgg gtgaatcagc gcttcaagaa gcaaaggacc aaaaaagtgt cgtcatcgga
     7921 tttgcaaatg aaaaacccta tgcatatcag gaagatggca aggtgacagg cgaagcggtg
     7981 gaaattgcaa agaaaatttt taaaaaccag ggaattacca atatcaaacc ggtgctaacg
     8041 gattttggtt ctttgatccc tggattaaaa gccggtcgct tcgatgtaat taccgcaggc
     8101 atgtatatta ctcctgagcg ttgtaagcag gtagcctttg ctaatccaga gtacaacatt
     8161 ggtgaggggt tagctgtgca gaagggaaat cccaaagata ttcacagcta taaggatgtg
     8221 gcaaatcaac ccgatacgac gatcgctgtg atgaaaggag caatcgaaga gtcctatatg
     8281 aaagattcgg gagtaaaaga gaatcagatt aagttcgttg cagatcaacc gactgcggtt
     8341 gcgcttctta attctggccg tgtggatggt gtgacgatga caggcccttc cttgcttgcc
     8401 attttacaat ctgcgaaagc aaagaatgtg tccgttgtag aagatttcaa gcaaccgatt
     8461 attgatggga aggagattaa ggggattggt gcggcggcct ttcggccaga ggataaggac
     8521 tttgtgaaga tatacaacga cggacttgct cagatgaagg aatcagggga actgtacgat
     8581 acgattcacc cctttggttt tacaaagaaa gatttacccg gcgatgagac cgcagaagaa
     8641 atctgtaagc agtaatcatg tagggtttta attctatgga aaggaagtga tcctgatcga
     8701 agtccatctt gagatattga agaatctgct cccgggtgca gttccaacga ttcagttgac
     8761 gattttggga gcgatgttag cctttgtact ctcatttatg ggaggacttg gtcgattatc
     8821 tcgttttgca gtcgtgcggg ggctggcagg gtttatcgtt gaatttttcc gtgggacatc
     8881 cttgttggtt caattatttt ggctatttta cgtactccct tttatgggaa ttcgcttaga
     8941 gtcaggaatg gtcggcctct tggctttggg tcttaattac ggagcatacg gatctgaagt
     9001 cgttcgtagt gcggtgttat ccgtaccaaa ggggcaacat gaagcaggga tcgcattaaa
     9061 tatgaccgcc ttccaacgaa tgcgctatat cattattcca caagcttttc gtaccatgat
     9121 tccttccttt ggaaatctgt taattgaatt gttaaagggg acagcactcg tctcattagt
     9181 caccattccg gagatgacct tcgaagcgtt ggtcatgcgg accagtgatg ctgcccatac
     9241 gcttgaaatc tttacttggc tacttctcat ttattttgcc atagcttatc cacttacgat
     9301 gctgatgcgt tgggtcgagc gtcgatattc tctagggagg ttataaccag tgatttggga
     9361 ttggcagtat gcctttgaaa ttttgccccg actccttaaa gtcgctcatg tcacgattac
     9421 ggcaacccta ggggcctttg ttgtcgccat gatattgggg ttggttttgg catttgcacg
     9481 tcgttcaaca aaccggttgc tttctggagt aacctatttt gtcgtcgaat ttattcgctc
     9541 tacgccccct tttgtacagt tgttctttct ctttttcgta atgccgcaat ttgggatcac
     9601 catgggcgca ttgatgacag ggattttggg tcttggtatc cattacagta ccttcttatc
     9661 cgaggtatat cgtgcgggaa ttgattccgt agacaaaggg caatgggaag cggccaccgc
     9721 ccttaatttt acctctcgac aaaaatgggt ccacattata ttgccacagg caattcgtcc
     9781 aatcatcccg gttatgggaa attacttgat caccttattt aaagagaccc ctatgttact
     9841 aggaatcaca gtggtagaga tgttgagtca agcacaaatt attggctccc agagcttccg
     9901 ttatacggaa ccaatcacga tggtggggat tttcttccta attctgagtt atggggcatc
     9961 cctacttgtc aaacggatcg aactgcggat ggatattaat cgtcgctagt acgtcaaaag
    10021 gaggaattta cgtgagcaat atacaatcaa cggaacgacc ccatacggag gcgcccatcg
    10081 aagggaaaga tccgattgtg cgttttaaaa acattagtaa atcctttggg gatttgcagg
    10141 tgttaaatgg agttgatttt gatttatacc caggagagcg agtcgcgatc atcggcccca
    10201 gtggttcagg aaaaacgacc atggcacgac tcctaatgac attggaggag ccgacggatg
    10261 ggtttattga agtcgatggc cgacaattgt ggcataaaga agtgaatggc aactttaaaa
    10321 aggccgatga aaaacacttg catgaggttc ggggagatat tgggatggtt tttcaacact
    10381 ttca
//
